AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07231

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07231.t1 MTR_1g088260 96.721 122 4 0 1 122 7 128 4.68e-83 239
MS.gene07231.t1 MTR_7g108775 61.157 121 46 1 1 121 42 161 2.82e-48 153
MS.gene07231.t1 MTR_7g108775 60.656 122 45 1 1 122 42 160 1.00e-47 150
MS.gene07231.t1 MTR_1g088250 50.000 118 54 2 2 114 36 153 7.29e-40 130
MS.gene07231.t1 MTR_6g086680 30.769 104 69 2 1 101 46 149 4.66e-16 70.1
MS.gene07231.t1 MTR_8g085580 33.010 103 64 3 2 101 42 142 4.10e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07231.t1 AT4G02360 63.636 110 40 0 2 111 41 150 4.87e-47 149
MS.gene07231.t1 AT1G02816 58.621 116 47 1 1 115 43 158 1.47e-46 148
MS.gene07231.t1 AT4G02370 60.000 110 43 1 1 109 43 152 2.75e-45 145
MS.gene07231.t1 AT1G02813 51.887 106 51 0 2 107 37 142 8.80e-37 122
MS.gene07231.t1 AT3G07460 35.246 122 64 6 1 118 41 151 6.14e-13 62.4
MS.gene07231.t1 AT3G07460 35.246 122 64 6 1 118 41 151 1.92e-12 62.4

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTACTTCTGCTGGTTT+TGG 0.248892 1.4:+70128428 MS.gene07231:CDS
TGTTGAAGTTTCTTCAATTT+AGG 0.268351 1.4:+70128526 MS.gene07231:CDS
ATTGACCTGTTTCTCTGTTA+AGG 0.276277 1.4:-70128214 None:intergenic
TTGACCTGTTTCTCTGTTAA+GGG 0.318122 1.4:-70128213 None:intergenic
GAATACCCAATTGCTCCTTT+TGG 0.335755 1.4:-70128191 None:intergenic
CTTCTTCCAAAAGGAGCAAT+TGG 0.352750 1.4:+70128185 MS.gene07231:CDS
TCTGTTGGGATTACTTCTGC+TGG 0.400761 1.4:+70128422 MS.gene07231:CDS
GAATGCCCTCAGTGTGGTTG+TGG 0.403933 1.4:+70128467 MS.gene07231:CDS
TATTCCCTTAACAGAGAAAC+AGG 0.430305 1.4:+70128209 MS.gene07231:CDS
GTGGTGTGATTTCGCAGAAT+AGG 0.437695 1.4:+70128309 MS.gene07231:CDS
AACCACACTGAGGGCATTCA+AGG 0.446234 1.4:-70128463 None:intergenic
CCAATGGGTCTTCTTCCAAA+AGG 0.464533 1.4:+70128176 None:intergenic
GATAATCATCTGAGGTTAAA+CGG 0.466866 1.4:+70128500 MS.gene07231:CDS
AATGCCCTCAGTGTGGTTGT+GGG 0.473689 1.4:+70128468 MS.gene07231:CDS
TATTGTTGAGGTTTCTCGTA+AGG 0.475808 1.4:+70128382 MS.gene07231:CDS
TTCTTCCAAAAGGAGCAATT+GGG 0.488774 1.4:+70128186 MS.gene07231:CDS
CCACAAAAGCAAAATCTTGA+CGG 0.495996 1.4:-70128354 None:intergenic
ATTGTTGAGGTTTCTCGTAA+GGG 0.528099 1.4:+70128383 MS.gene07231:CDS
GAATAGGCTTTATAAACTCA+AGG 0.537506 1.4:+70128325 MS.gene07231:CDS
TTTGTGGTTGAATATTGTTG+AGG 0.605032 1.4:+70128370 MS.gene07231:CDS
AATAGGCTTTATAAACTCAA+GGG 0.613735 1.4:+70128326 MS.gene07231:CDS
AGCTACAAATCTACTATCAG+TGG 0.618877 1.4:+70128290 MS.gene07231:CDS
TTGATTGCGATAATCATCTG+AGG 0.632187 1.4:+70128492 MS.gene07231:CDS
CAAACCCACAACCACACTGA+GGG 0.636398 1.4:-70128472 None:intergenic
TTCCTTGAATGCCCTCAGTG+TGG 0.651724 1.4:+70128461 MS.gene07231:CDS
TCAAACCCACAACCACACTG+AGG 0.728360 1.4:-70128473 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTGATTTTTCTGT+TGG + chr1.4:70128407-70128426 MS.gene07231:CDS 20.0%
!!! ATGATATTGATTTTTCTGTT+GGG + chr1.4:70128408-70128427 MS.gene07231:CDS 20.0%
!! AATAGGCTTTATAAACTCAA+GGG + chr1.4:70128326-70128345 MS.gene07231:CDS 25.0%
TTTGTGGTTGAATATTGTTG+AGG + chr1.4:70128370-70128389 MS.gene07231:CDS 30.0%
! GAATAGGCTTTATAAACTCA+AGG + chr1.4:70128325-70128344 MS.gene07231:CDS 30.0%
! GATAATCATCTGAGGTTAAA+CGG + chr1.4:70128500-70128519 MS.gene07231:CDS 30.0%
AGCTACAAATCTACTATCAG+TGG + chr1.4:70128290-70128309 MS.gene07231:CDS 35.0%
ATTGTTGAGGTTTCTCGTAA+GGG + chr1.4:70128383-70128402 MS.gene07231:CDS 35.0%
CCACAAAAGCAAAATCTTGA+CGG - chr1.4:70128357-70128376 None:intergenic 35.0%
TATTCCCTTAACAGAGAAAC+AGG + chr1.4:70128209-70128228 MS.gene07231:CDS 35.0%
TATTGTTGAGGTTTCTCGTA+AGG + chr1.4:70128382-70128401 MS.gene07231:CDS 35.0%
TTCTTCCAAAAGGAGCAATT+GGG + chr1.4:70128186-70128205 MS.gene07231:CDS 35.0%
TTGATTGCGATAATCATCTG+AGG + chr1.4:70128492-70128511 MS.gene07231:CDS 35.0%
! ATTGACCTGTTTCTCTGTTA+AGG - chr1.4:70128217-70128236 None:intergenic 35.0%
! TTGACCTGTTTCTCTGTTAA+GGG - chr1.4:70128216-70128235 None:intergenic 35.0%
CTTCTTCCAAAAGGAGCAAT+TGG + chr1.4:70128185-70128204 MS.gene07231:CDS 40.0%
! GAATACCCAATTGCTCCTTT+TGG - chr1.4:70128194-70128213 None:intergenic 40.0%
!! CCGTCAAGATTTTGCTTTTG+TGG + chr1.4:70128354-70128373 MS.gene07231:CDS 40.0%
GTGGTGTGATTTCGCAGAAT+AGG + chr1.4:70128309-70128328 MS.gene07231:CDS 45.0%
TCTGTTGGGATTACTTCTGC+TGG + chr1.4:70128422-70128441 MS.gene07231:CDS 45.0%
!!! GGGATTACTTCTGCTGGTTT+TGG + chr1.4:70128428-70128447 MS.gene07231:CDS 45.0%
AATGCCCTCAGTGTGGTTGT+GGG + chr1.4:70128468-70128487 MS.gene07231:CDS 50.0%
CAAACCCACAACCACACTGA+GGG - chr1.4:70128475-70128494 None:intergenic 50.0%
TCAAACCCACAACCACACTG+AGG - chr1.4:70128476-70128495 None:intergenic 50.0%
TTCCTTGAATGCCCTCAGTG+TGG + chr1.4:70128461-70128480 MS.gene07231:CDS 50.0%
!! AACCACACTGAGGGCATTCA+AGG - chr1.4:70128466-70128485 None:intergenic 50.0%
GAATGCCCTCAGTGTGGTTG+TGG + chr1.4:70128467-70128486 MS.gene07231:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 70128179 70128547 70128179 ID=MS.gene07231
chr1.4 mRNA 70128179 70128547 70128179 ID=MS.gene07231.t1;Parent=MS.gene07231
chr1.4 exon 70128179 70128547 70128179 ID=MS.gene07231.t1.exon1;Parent=MS.gene07231.t1
chr1.4 CDS 70128179 70128547 70128179 ID=cds.MS.gene07231.t1;Parent=MS.gene07231.t1
Gene Sequence

>MS.gene07231

ATGGGTCTTCTTCCAAAAGGAGCAATTGGGTATTCCCTTAACAGAGAAACAGGTCAATTTTCTGTGTATTTCGAGAAAACTTGTAGCTTTGTTATTGAAAGCTATACACTTAGCTACAAATCTACTATCAGTGGTGTGATTTCGCAGAATAGGCTTTATAAACTCAAGGGTGTTTCCGTCAAGATTTTGCTTTTGTGGTTGAATATTGTTGAGGTTTCTCGTAAGGGTAATGATATTGATTTTTCTGTTGGGATTACTTCTGCTGGTTTTGGAGTTGAGAATTTCCTTGAATGCCCTCAGTGTGGTTGTGGGTTTGATTGCGATAATCATCTGAGGTTAAACGGTGATGTTGAAGTTTCTTCAATTTAG
Protein sequence

>MS.gene07231.t1

MGLLPKGAIGYSLNRETGQFSVYFEKTCSFVIESYTLSYKSTISGVISQNRLYKLKGVSVKILLLWLNIVEVSRKGNDIDFSVGITSAGFGVENFLECPQCGCGFDCDNHLRLNGDVEVSSI