Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072907.t1 | XP_003603418.1 | 91.6 | 190 | 14 | 1 | 1 | 188 | 1 | 190 | 3.20E-71 | 278.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072907.t1 | Q9SUV1 | 54.6 | 194 | 70 | 4 | 1 | 188 | 1 | 182 | 7.6e-37 | 155.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072907.t1 | G7JB41 | 91.6 | 190 | 14 | 1 | 1 | 188 | 1 | 190 | 2.3e-71 | 278.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049703 | MS.gene072907 | -0.804376 | 2.23E-49 | -1.69E-46 |
| MS.gene04984 | MS.gene072907 | 0.804712 | 1.89E-49 | -1.69E-46 |
| MS.gene050629 | MS.gene072907 | -0.83304 | 6.70E-56 | -1.69E-46 |
| MS.gene052302 | MS.gene072907 | 0.805039 | 1.62E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene072907 | 0.805945 | 1.04E-49 | -1.69E-46 |
| MS.gene052856 | MS.gene072907 | 0.832872 | 7.38E-56 | -1.69E-46 |
| MS.gene052858 | MS.gene072907 | 0.808477 | 3.04E-50 | -1.69E-46 |
| MS.gene052860 | MS.gene072907 | 0.810677 | 1.02E-50 | -1.69E-46 |
| MS.gene053437 | MS.gene072907 | 0.805015 | 1.64E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene072907 | 0.804774 | 1.84E-49 | -1.69E-46 |
| MS.gene055174 | MS.gene072907 | 0.807647 | 4.56E-50 | -1.69E-46 |
| MS.gene058182 | MS.gene072907 | 0.802608 | 5.18E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene072907 | 0.808456 | 3.07E-50 | -1.69E-46 |
| MS.gene060869 | MS.gene072907 | 0.815342 | 9.71E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072907.t1 | MTR_3g107510 | 94.211 | 190 | 9 | 1 | 1 | 188 | 1 | 190 | 4.14e-127 | 364 |
| MS.gene072907.t1 | MTR_1g012030 | 59.113 | 203 | 67 | 4 | 2 | 188 | 5 | 207 | 9.64e-62 | 197 |
| MS.gene072907.t1 | MTR_4g114320 | 53.107 | 177 | 65 | 6 | 25 | 188 | 21 | 192 | 2.86e-46 | 157 |
| MS.gene072907.t1 | MTR_4g005330 | 45.070 | 71 | 39 | 0 | 117 | 187 | 63 | 133 | 6.15e-17 | 78.2 |
| MS.gene072907.t1 | MTR_4g005330 | 46.970 | 66 | 35 | 0 | 122 | 187 | 2 | 67 | 3.00e-16 | 75.5 |
| MS.gene072907.t1 | MTR_3g074490 | 38.356 | 73 | 45 | 0 | 114 | 186 | 110 | 182 | 1.50e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072907.t1 | AT4G32400 | 51.546 | 194 | 76 | 4 | 1 | 188 | 1 | 182 | 7.34e-52 | 171 |
| MS.gene072907.t1 | AT3G20240 | 45.070 | 71 | 39 | 0 | 118 | 188 | 50 | 120 | 4.96e-16 | 75.5 |
Find 57 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCACTAAAAGGAAATTT+AGG | 0.125546 | 3.4:-93373809 | None:intergenic |
| GATTCTAATTCCCCTGAAAA+TGG | 0.247936 | 3.4:+93373779 | MS.gene072907:CDS |
| TTAACGTATAATTCACTAAA+AGG | 0.290618 | 3.4:-93373818 | None:intergenic |
| TGGATGGAAGGGTTTGTTTA+GGG | 0.298083 | 3.4:+93374111 | MS.gene072907:CDS |
| ATTCACTAAAAGGAAATTTA+GGG | 0.305139 | 3.4:-93373808 | None:intergenic |
| TGGCACCTTTGGAGACTATT+AGG | 0.317047 | 3.4:+93374020 | MS.gene072907:CDS |
| ATGGATGGAAGGGTTTGTTT+AGG | 0.338034 | 3.4:+93374110 | MS.gene072907:CDS |
| TAAGGAATCCTTCTATTAGA+AGG | 0.342750 | 3.4:+93373954 | MS.gene072907:CDS |
| TAGAACCACTGTGGCACCTT+TGG | 0.351090 | 3.4:+93374009 | MS.gene072907:CDS |
| GAAATCTTGTTAATGTCATT+CGG | 0.353717 | 3.4:+93374134 | MS.gene072907:CDS |
| TTTGAGCCTAATGGTGTTAC+GGG | 0.368589 | 3.4:+93373863 | MS.gene072907:CDS |
| AGTGTTGGTCAAGTGGGAAT+AGG | 0.372936 | 3.4:+93373728 | MS.gene072907:CDS |
| AAATCTTGTTAATGTCATTC+GGG | 0.378614 | 3.4:+93374135 | MS.gene072907:CDS |
| CTCTGGAAATTTGAGCCTAA+TGG | 0.383482 | 3.4:+93373854 | MS.gene072907:CDS |
| GTGTTGGTCAAGTGGGAATA+GGG | 0.395032 | 3.4:+93373729 | MS.gene072907:CDS |
| GGTCAAGTGGGAATAGGGTT+TGG | 0.403207 | 3.4:+93373734 | MS.gene072907:CDS |
| GACTATTAGGACTCATTTGA+TGG | 0.419164 | 3.4:+93374033 | MS.gene072907:CDS |
| CTACACAACCTTCTAATAGA+AGG | 0.424911 | 3.4:-93373962 | None:intergenic |
| GAATCAGAAGGGTTTGGTTG+TGG | 0.427235 | 3.4:-93373761 | None:intergenic |
| GAATTAGAATCAGAAGGGTT+TGG | 0.431685 | 3.4:-93373767 | None:intergenic |
| ATTTGAGCCTAATGGTGTTA+CGG | 0.440900 | 3.4:+93373862 | MS.gene072907:CDS |
| CCTAATGGTGTTACGGGCAA+GGG | 0.453681 | 3.4:+93373869 | MS.gene072907:CDS |
| GGTGGTTTGTTTGCTAGTGT+TGG | 0.463456 | 3.4:+93373713 | MS.gene072907:CDS |
| CTCTTTGATGACAAAAGAGA+TGG | 0.481584 | 3.4:+93373641 | MS.gene072907:CDS |
| TTGTGTAGTGGAGCAATTGC+TGG | 0.481763 | 3.4:+93373977 | MS.gene072907:CDS |
| CAGGATATTATGAAAACTGA+TGG | 0.484050 | 3.4:+93374091 | MS.gene072907:CDS |
| GCCTAATGGTGTTACGGGCA+AGG | 0.486297 | 3.4:+93373868 | MS.gene072907:CDS |
| CATGAGGATTACTATTATCC+TGG | 0.492331 | 3.4:+93373692 | MS.gene072907:CDS |
| GGAAGGTGAACAAGTTGTTA+AGG | 0.501044 | 3.4:+93373892 | MS.gene072907:CDS |
| CCCTTGCCCGTAACACCATT+AGG | 0.507798 | 3.4:-93373869 | None:intergenic |
| GGATGGAAGGGTTTGTTTAG+GGG | 0.528599 | 3.4:+93374112 | MS.gene072907:CDS |
| ATGAAAACTGATGGATGGAA+GGG | 0.528727 | 3.4:+93374100 | MS.gene072907:CDS |
| TTAAGCTCAAGATTAAGATA+AGG | 0.531801 | 3.4:+93373936 | MS.gene072907:CDS |
| TATGAAAACTGATGGATGGA+AGG | 0.533911 | 3.4:+93374099 | MS.gene072907:CDS |
| TCAGGGGAATTAGAATCAGA+AGG | 0.536789 | 3.4:-93373773 | None:intergenic |
| TGCGCCTAGCAAGGCTATTG+AGG | 0.550247 | 3.4:+93374159 | MS.gene072907:CDS |
| GTTTGCTAGTGTTGGTCAAG+TGG | 0.552428 | 3.4:+93373721 | MS.gene072907:CDS |
| TTTGCTAGTGTTGGTCAAGT+GGG | 0.555292 | 3.4:+93373722 | MS.gene072907:CDS |
| CATTCGGGTTGCGCCTAGCA+AGG | 0.557121 | 3.4:+93374150 | MS.gene072907:CDS |
| TTAAGTATATTCAATCACTC+TGG | 0.561876 | 3.4:+93373837 | MS.gene072907:CDS |
| CATTTGATGGTGGGAAGTAG+TGG | 0.562649 | 3.4:+93374046 | MS.gene072907:CDS |
| ATTAGGACTCATTTGATGGT+GGG | 0.563542 | 3.4:+93374037 | MS.gene072907:CDS |
| CAGGGGAATTAGAATCAGAA+GGG | 0.563815 | 3.4:-93373772 | None:intergenic |
| ATTACCTCAATAGCCTTGCT+AGG | 0.568427 | 3.4:-93374163 | None:intergenic |
| TATTAGGACTCATTTGATGG+TGG | 0.584361 | 3.4:+93374036 | MS.gene072907:CDS |
| TCTATTAGAAGGTTGTGTAG+TGG | 0.586868 | 3.4:+93373965 | MS.gene072907:CDS |
| TCTTTGATGACAAAAGAGAT+GGG | 0.588905 | 3.4:+93373642 | MS.gene072907:CDS |
| CTAATGGTGTTACGGGCAAG+GGG | 0.590038 | 3.4:+93373870 | MS.gene072907:CDS |
| TTCAAATTTAGGGTCTCATG+AGG | 0.600906 | 3.4:+93373676 | MS.gene072907:CDS |
| ATATTATGAAAACTGATGGA+TGG | 0.609112 | 3.4:+93374095 | MS.gene072907:CDS |
| GAGGATTACTATTATCCTGG+TGG | 0.610245 | 3.4:+93373695 | MS.gene072907:CDS |
| TGAGTCCTAATAGTCTCCAA+AGG | 0.641578 | 3.4:-93374025 | None:intergenic |
| ACACTAGCAAACAAACCACC+AGG | 0.654663 | 3.4:-93373710 | None:intergenic |
| TAATGGTGTTACGGGCAAGG+GGG | 0.685871 | 3.4:+93373871 | MS.gene072907:CDS |
| AGTCTCCAAAGGTGCCACAG+TGG | 0.705525 | 3.4:-93374014 | None:intergenic |
| AATATCCTGGAAAACATCAG+CGG | 0.708839 | 3.4:-93374077 | None:intergenic |
| TGCAATTTCTAGAACCACTG+TGG | 0.742671 | 3.4:+93374000 | MS.gene072907:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCACTAAAAGGAAATTT+AGG | - | chr3.4:93373812-93373831 | None:intergenic | 20.0% |
| !! | ATTCACTAAAAGGAAATTTA+GGG | - | chr3.4:93373811-93373830 | None:intergenic | 20.0% |
| !! | TTAACGTATAATTCACTAAA+AGG | - | chr3.4:93373821-93373840 | None:intergenic | 20.0% |
| !!! | TTTTCTCTGTTTCAAATTTA+GGG | + | chr3.4:93373666-93373685 | MS.gene072907:CDS | 20.0% |
| !!! | TTTTTCTCTGTTTCAAATTT+AGG | + | chr3.4:93373665-93373684 | MS.gene072907:CDS | 20.0% |
| ! | AAATCTTGTTAATGTCATTC+GGG | + | chr3.4:93374135-93374154 | MS.gene072907:CDS | 25.0% |
| ! | ATATTATGAAAACTGATGGA+TGG | + | chr3.4:93374095-93374114 | MS.gene072907:CDS | 25.0% |
| ! | GAAATCTTGTTAATGTCATT+CGG | + | chr3.4:93374134-93374153 | MS.gene072907:CDS | 25.0% |
| ! | GTTAAGGTGAAGAATAAAAA+TGG | + | chr3.4:93373908-93373927 | MS.gene072907:CDS | 25.0% |
| ! | TTAAGCTCAAGATTAAGATA+AGG | + | chr3.4:93373936-93373955 | MS.gene072907:CDS | 25.0% |
| ! | TTAAGTATATTCAATCACTC+TGG | + | chr3.4:93373837-93373856 | MS.gene072907:CDS | 25.0% |
| AAGGTGAAGAATAAAAATGG+TGG | + | chr3.4:93373911-93373930 | MS.gene072907:CDS | 30.0% | |
| AGGTGAAGAATAAAAATGGT+GGG | + | chr3.4:93373912-93373931 | MS.gene072907:CDS | 30.0% | |
| CAGGATATTATGAAAACTGA+TGG | + | chr3.4:93374091-93374110 | MS.gene072907:CDS | 30.0% | |
| TAAGGAATCCTTCTATTAGA+AGG | + | chr3.4:93373954-93373973 | MS.gene072907:CDS | 30.0% | |
| ! | CATCAGTTTTCATAATATCC+TGG | - | chr3.4:93374093-93374112 | None:intergenic | 30.0% |
| ! | TCTTTGATGACAAAAGAGAT+GGG | + | chr3.4:93373642-93373661 | MS.gene072907:CDS | 30.0% |
| AATATCCTGGAAAACATCAG+CGG | - | chr3.4:93374080-93374099 | None:intergenic | 35.0% | |
| CATGAGGATTACTATTATCC+TGG | + | chr3.4:93373692-93373711 | MS.gene072907:CDS | 35.0% | |
| CTACACAACCTTCTAATAGA+AGG | - | chr3.4:93373965-93373984 | None:intergenic | 35.0% | |
| GAATTAGAATCAGAAGGGTT+TGG | - | chr3.4:93373770-93373789 | None:intergenic | 35.0% | |
| GACTATTAGGACTCATTTGA+TGG | + | chr3.4:93374033-93374052 | MS.gene072907:CDS | 35.0% | |
| GATTCTAATTCCCCTGAAAA+TGG | + | chr3.4:93373779-93373798 | MS.gene072907:CDS | 35.0% | |
| TATTAGGACTCATTTGATGG+TGG | + | chr3.4:93374036-93374055 | MS.gene072907:CDS | 35.0% | |
| TCTATTAGAAGGTTGTGTAG+TGG | + | chr3.4:93373965-93373984 | MS.gene072907:CDS | 35.0% | |
| ! | ATGAAAACTGATGGATGGAA+GGG | + | chr3.4:93374100-93374119 | MS.gene072907:CDS | 35.0% |
| ! | ATTAGGACTCATTTGATGGT+GGG | + | chr3.4:93374037-93374056 | MS.gene072907:CDS | 35.0% |
| ! | ATTTGAGCCTAATGGTGTTA+CGG | + | chr3.4:93373862-93373881 | MS.gene072907:CDS | 35.0% |
| ! | CTCTTTGATGACAAAAGAGA+TGG | + | chr3.4:93373641-93373660 | MS.gene072907:CDS | 35.0% |
| ! | CTCTTTTGTCATCAAAGAGT+TGG | - | chr3.4:93373640-93373659 | None:intergenic | 35.0% |
| ! | TATGAAAACTGATGGATGGA+AGG | + | chr3.4:93374099-93374118 | MS.gene072907:CDS | 35.0% |
| ! | TTCAAATTTAGGGTCTCATG+AGG | + | chr3.4:93373676-93373695 | MS.gene072907:CDS | 35.0% |
| CAGGGGAATTAGAATCAGAA+GGG | - | chr3.4:93373775-93373794 | None:intergenic | 40.0% | |
| GAGGATTACTATTATCCTGG+TGG | + | chr3.4:93373695-93373714 | MS.gene072907:CDS | 40.0% | |
| GGAAGGTGAACAAGTTGTTA+AGG | + | chr3.4:93373892-93373911 | MS.gene072907:CDS | 40.0% | |
| TCAGGGGAATTAGAATCAGA+AGG | - | chr3.4:93373776-93373795 | None:intergenic | 40.0% | |
| TGAGTCCTAATAGTCTCCAA+AGG | - | chr3.4:93374028-93374047 | None:intergenic | 40.0% | |
| ! | ATGGATGGAAGGGTTTGTTT+AGG | + | chr3.4:93374110-93374129 | MS.gene072907:CDS | 40.0% |
| ! | ATTACCTCAATAGCCTTGCT+AGG | - | chr3.4:93374166-93374185 | None:intergenic | 40.0% |
| ! | CTCTGGAAATTTGAGCCTAA+TGG | + | chr3.4:93373854-93373873 | MS.gene072907:CDS | 40.0% |
| ! | TAGGGTCATCACCATTTTCA+GGG | - | chr3.4:93373793-93373812 | None:intergenic | 40.0% |
| ! | TGGATGGAAGGGTTTGTTTA+GGG | + | chr3.4:93374111-93374130 | MS.gene072907:CDS | 40.0% |
| ! | TTAGGGTCATCACCATTTTC+AGG | - | chr3.4:93373794-93373813 | None:intergenic | 40.0% |
| !! | TGCAATTTCTAGAACCACTG+TGG | + | chr3.4:93374000-93374019 | MS.gene072907:CDS | 40.0% |
| !! | TTTGAGCCTAATGGTGTTAC+GGG | + | chr3.4:93373863-93373882 | MS.gene072907:CDS | 40.0% |
| !! | TTTGCTAGTGTTGGTCAAGT+GGG | + | chr3.4:93373722-93373741 | MS.gene072907:CDS | 40.0% |
| ACACTAGCAAACAAACCACC+AGG | - | chr3.4:93373713-93373732 | None:intergenic | 45.0% | |
| ! | AGGGTCATCACCATTTTCAG+GGG | - | chr3.4:93373792-93373811 | None:intergenic | 45.0% |
| ! | GAATCAGAAGGGTTTGGTTG+TGG | - | chr3.4:93373764-93373783 | None:intergenic | 45.0% |
| ! | GGATGGAAGGGTTTGTTTAG+GGG | + | chr3.4:93374112-93374131 | MS.gene072907:CDS | 45.0% |
| ! | GTGTTGGTCAAGTGGGAATA+GGG | + | chr3.4:93373729-93373748 | MS.gene072907:CDS | 45.0% |
| ! | TGGCACCTTTGGAGACTATT+AGG | + | chr3.4:93374020-93374039 | MS.gene072907:CDS | 45.0% |
| ! | TTCTACCGCTGATGTTTTCC+AGG | + | chr3.4:93374072-93374091 | MS.gene072907:CDS | 45.0% |
| ! | TTGTGTAGTGGAGCAATTGC+TGG | + | chr3.4:93373977-93373996 | MS.gene072907:CDS | 45.0% |
| !! | AGTGTTGGTCAAGTGGGAAT+AGG | + | chr3.4:93373728-93373747 | MS.gene072907:CDS | 45.0% |
| !! | CATTTGATGGTGGGAAGTAG+TGG | + | chr3.4:93374046-93374065 | MS.gene072907:CDS | 45.0% |
| !! | GGTGGTTTGTTTGCTAGTGT+TGG | + | chr3.4:93373713-93373732 | MS.gene072907:CDS | 45.0% |
| !! | GTTTGCTAGTGTTGGTCAAG+TGG | + | chr3.4:93373721-93373740 | MS.gene072907:CDS | 45.0% |
| GGTCAAGTGGGAATAGGGTT+TGG | + | chr3.4:93373734-93373753 | MS.gene072907:CDS | 50.0% | |
| TAGAACCACTGTGGCACCTT+TGG | + | chr3.4:93374009-93374028 | MS.gene072907:CDS | 50.0% | |
| !! | CCTAATGGTGTTACGGGCAA+GGG | + | chr3.4:93373869-93373888 | MS.gene072907:CDS | 50.0% |
| !! | CTAATGGTGTTACGGGCAAG+GGG | + | chr3.4:93373870-93373889 | MS.gene072907:CDS | 50.0% |
| !! | TAATGGTGTTACGGGCAAGG+GGG | + | chr3.4:93373871-93373890 | MS.gene072907:CDS | 50.0% |
| CCCTTGCCCGTAACACCATT+AGG | - | chr3.4:93373872-93373891 | None:intergenic | 55.0% | |
| TGCGCCTAGCAAGGCTATTG+AGG | + | chr3.4:93374159-93374178 | MS.gene072907:CDS | 55.0% | |
| !! | AGTCTCCAAAGGTGCCACAG+TGG | - | chr3.4:93374017-93374036 | None:intergenic | 55.0% |
| !! | GCCTAATGGTGTTACGGGCA+AGG | + | chr3.4:93373868-93373887 | MS.gene072907:CDS | 55.0% |
| !! | CATTCGGGTTGCGCCTAGCA+AGG | + | chr3.4:93374150-93374169 | MS.gene072907:CDS | 60.0% |
| ! | GGTGTTACGGGCAAGGGGGA+AGG | + | chr3.4:93373875-93373894 | MS.gene072907:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 93373620 | 93374225 | 93373620 | ID=MS.gene072907 |
| chr3.4 | mRNA | 93373620 | 93374225 | 93373620 | ID=MS.gene072907.t1;Parent=MS.gene072907 |
| chr3.4 | exon | 93373620 | 93374225 | 93373620 | ID=MS.gene072907.t1.exon1;Parent=MS.gene072907.t1 |
| chr3.4 | CDS | 93373620 | 93374225 | 93373620 | ID=cds.MS.gene072907.t1;Parent=MS.gene072907.t1 |
| Gene Sequence |
| Protein sequence |
