AlfalfaGEDB | MS.gene073225

M. sativa cultivar XinJiangDaYe / MS.gene073225

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073225.t1	XP_003605725.1	83.3	138	15	1	1	130	1	138	7.20E-48	199.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073225.t1	G7JSI0	83.3	138	15	1	1	130	1	138	5.2e-48	199.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene073225	0.847941	8.41E-60	-1.69E-46
MS.gene050584	MS.gene073225	0.882209	1.26E-70	-1.69E-46
MS.gene050585	MS.gene073225	0.862816	3.88E-64	-1.69E-46
MS.gene050586	MS.gene073225	0.817261	3.61E-52	-1.69E-46
MS.gene050587	MS.gene073225	0.855879	4.69E-62	-1.69E-46
MS.gene050807	MS.gene073225	0.801563	8.49E-49	-1.69E-46
MS.gene051789	MS.gene073225	0.854696	1.04E-61	-1.69E-46
MS.gene052142	MS.gene073225	0.822731	2.02E-53	-1.69E-46
MS.gene053074	MS.gene073225	0.855862	4.75E-62	-1.69E-46
MS.gene053383	MS.gene073225	0.817946	2.53E-52	-1.69E-46
MS.gene05456	MS.gene073225	0.862388	5.25E-64	-1.69E-46
MS.gene055359	MS.gene073225	0.822039	2.93E-53	-1.69E-46
MS.gene059006	MS.gene073225	0.854408	1.26E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073225.t1	MTR_4g037560	81.884	138	17	1	1	130	1	138	1.07e-63	191
MS.gene073225.t1	MTR_4g037480	80.916	131	20	2	1	130	1	127	1.64e-59	179
MS.gene073225.t1	MTR_2g090185	54.472	123	47	4	1	120	1	117	3.85e-28	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 43 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATCCAGACATTGGACATTT+AGG	0.139849	4.3:-54818111	MS.gene073225:CDS
CTCTTTCTTGTCGTCGTTGT+TGG	0.251145	4.3:+54818173	None:intergenic
ATCCAGACATTGGACATTTA+GGG	0.287181	4.3:-54818110	MS.gene073225:CDS
CCACCAATGAATGGGGTATT+AGG	0.288576	4.3:+54818063	None:intergenic
GGCTTTGAGTTTCCGAATCC+TGG	0.342783	4.3:-54817952	MS.gene073225:CDS
CCACCGACACCACCACTTCC+AGG	0.370147	4.3:+54817934	None:intergenic
GGAAACTCAAAGCCAGGGTT+TGG	0.384213	4.3:+54817961	None:intergenic
TCAGGATCGGGCTCAGGATC+AGG	0.405669	4.3:-54818030	MS.gene073225:CDS
ACCCCATTCATTGGTGGCAC+TGG	0.413289	4.3:-54818057	MS.gene073225:CDS
TCGGGTCACAGTTATGTTCC+TGG	0.415011	4.3:-54818003	MS.gene073225:CDS
ATTGGTGGCACTGGAGGATC+AGG	0.420721	4.3:-54818048	MS.gene073225:CDS
CTCCAGTGCCACCAATGAAT+GGG	0.420754	4.3:+54818055	None:intergenic
AACCCTAAATGTCCAATGTC+TGG	0.424701	4.3:+54818108	None:intergenic
CCTCCAGTGCCACCAATGAA+TGG	0.446637	4.3:+54818054	None:intergenic
GGCACTGGAGGATCAGGATC+GGG	0.449576	4.3:-54818042	MS.gene073225:CDS
ACTCCTAATACCCCATTCAT+TGG	0.459225	4.3:-54818066	MS.gene073225:CDS
GGCTCAGGATCAGGATCATC+GGG	0.463153	4.3:-54818021	MS.gene073225:CDS
ACACCACCACTTCCAGGATT+CGG	0.485620	4.3:+54817940	None:intergenic
TGGCACTGGAGGATCAGGAT+CGG	0.491247	4.3:-54818043	MS.gene073225:CDS
GGAGGATCAGGATCGGGCTC+AGG	0.506667	4.3:-54818036	MS.gene073225:CDS
TTTCTGTTCAAGCACGAACA+TGG	0.515179	4.3:-54818144	MS.gene073225:CDS
TTAGGGTTCCCACCACACTT+TGG	0.520108	4.3:-54818093	MS.gene073225:CDS
GGATTCGGAAACTCAAAGCC+AGG	0.521240	4.3:+54817955	None:intergenic
GAGTTTCCGAATCCTGGAAG+TGG	0.521602	4.3:-54817946	MS.gene073225:CDS
ACAACACAAGAAGCAATTGC+TGG	0.522717	4.3:-54818216	MS.gene073225:CDS
CCATTCATTGGTGGCACTGG+AGG	0.535115	4.3:-54818054	MS.gene073225:CDS
GGGCTCAGGATCAGGATCAT+CGG	0.548582	4.3:-54818022	MS.gene073225:CDS
AAACTACATTATCCAGACAT+TGG	0.559408	4.3:-54818120	MS.gene073225:CDS
TTCTGTTCAAGCACGAACAT+GGG	0.565643	4.3:-54818143	MS.gene073225:CDS
TTGCTTCTTGTGTTGTGAGA+AGG	0.565897	4.3:+54818223	None:intergenic
GACACATTTGTTCCAAACCC+TGG	0.566936	4.3:-54817973	MS.gene073225:CDS
CCTAATACCCCATTCATTGG+TGG	0.569848	4.3:-54818063	MS.gene073225:CDS
ACAAATGTGTCATCGCCACC+AGG	0.593265	4.3:+54817985	None:intergenic
GAGTAAGTCCAAAGTGTGGT+GGG	0.596087	4.3:+54818085	None:intergenic
GGAGTAAGTCCAAAGTGTGG+TGG	0.604268	4.3:+54818084	None:intergenic
CCTGGAAGTGGTGGTGTCGG+TGG	0.607001	4.3:-54817934	MS.gene073225:CDS
AATCCTGGAAGTGGTGGTGT+CGG	0.618339	4.3:-54817937	MS.gene073225:CDS
GATTCGGAAACTCAAAGCCA+GGG	0.619017	4.3:+54817956	None:intergenic
TCCAGTGCCACCAATGAATG+GGG	0.638319	4.3:+54818056	None:intergenic
TTTCCGAATCCTGGAAGTGG+TGG	0.658317	4.3:-54817943	MS.gene073225:CDS
TTAGGAGTAAGTCCAAAGTG+TGG	0.682645	4.3:+54818081	None:intergenic
TCGTGCTTGAACAGAAACTG+AGG	0.705299	4.3:+54818150	None:intergenic
GGTCACAGTTATGTTCCTGG+TGG	0.707662	4.3:-54818000	MS.gene073225:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACTACATTATCCAGACAT+TGG	-	chr4.3:54818062-54818081	MS.gene073225:CDS	30.0%
	ATCCAGACATTGGACATTTA+GGG	-	chr4.3:54818072-54818091	MS.gene073225:CDS	35.0%
	TATCCAGACATTGGACATTT+AGG	-	chr4.3:54818071-54818090	MS.gene073225:CDS	35.0%
	AACCCTAAATGTCCAATGTC+TGG	+	chr4.3:54818077-54818096	None:intergenic	40.0%
	ACAACACAAGAAGCAATTGC+TGG	-	chr4.3:54817966-54817985	MS.gene073225:CDS	40.0%
	ACTCCTAATACCCCATTCAT+TGG	-	chr4.3:54818116-54818135	MS.gene073225:CDS	40.0%
	TTAGGAGTAAGTCCAAAGTG+TGG	+	chr4.3:54818104-54818123	None:intergenic	40.0%
	TTCTGTTCAAGCACGAACAT+GGG	-	chr4.3:54818039-54818058	MS.gene073225:CDS	40.0%
	TTGCTTCTTGTGTTGTGAGA+AGG	+	chr4.3:54817962-54817981	None:intergenic	40.0%
	TTTCTGTTCAAGCACGAACA+TGG	-	chr4.3:54818038-54818057	MS.gene073225:CDS	40.0%
	CCTAATACCCCATTCATTGG+TGG	-	chr4.3:54818119-54818138	MS.gene073225:CDS	45.0%
	CTCTTTCTTGTCGTCGTTGT+TGG	+	chr4.3:54818012-54818031	None:intergenic	45.0%
	GACACATTTGTTCCAAACCC+TGG	-	chr4.3:54818209-54818228	MS.gene073225:CDS	45.0%
	GAGTAAGTCCAAAGTGTGGT+GGG	+	chr4.3:54818100-54818119	None:intergenic	45.0%
	GATTCGGAAACTCAAAGCCA+GGG	+	chr4.3:54818229-54818248	None:intergenic	45.0%
	TCGTGCTTGAACAGAAACTG+AGG	+	chr4.3:54818035-54818054	None:intergenic	45.0%
!	CCACCAATGAATGGGGTATT+AGG	+	chr4.3:54818122-54818141	None:intergenic	45.0%
	ACAAATGTGTCATCGCCACC+AGG	+	chr4.3:54818200-54818219	None:intergenic	50.0%
	ACACCACCACTTCCAGGATT+CGG	+	chr4.3:54818245-54818264	None:intergenic	50.0%
	CTCCAGTGCCACCAATGAAT+GGG	+	chr4.3:54818130-54818149	None:intergenic	50.0%
	GAGTTTCCGAATCCTGGAAG+TGG	-	chr4.3:54818236-54818255	MS.gene073225:CDS	50.0%
	GGAAACTCAAAGCCAGGGTT+TGG	+	chr4.3:54818224-54818243	None:intergenic	50.0%
	GGAGTAAGTCCAAAGTGTGG+TGG	+	chr4.3:54818101-54818120	None:intergenic	50.0%
	GGATTCGGAAACTCAAAGCC+AGG	+	chr4.3:54818230-54818249	None:intergenic	50.0%
	GGTCACAGTTATGTTCCTGG+TGG	-	chr4.3:54818182-54818201	MS.gene073225:CDS	50.0%
	TCCAGTGCCACCAATGAATG+GGG	+	chr4.3:54818129-54818148	None:intergenic	50.0%
	TCGGGTCACAGTTATGTTCC+TGG	-	chr4.3:54818179-54818198	MS.gene073225:CDS	50.0%
	TTAGGGTTCCCACCACACTT+TGG	-	chr4.3:54818089-54818108	MS.gene073225:CDS	50.0%
	TTTCCGAATCCTGGAAGTGG+TGG	-	chr4.3:54818239-54818258	MS.gene073225:CDS	50.0%
!	AATCCTGGAAGTGGTGGTGT+CGG	-	chr4.3:54818245-54818264	MS.gene073225:CDS	50.0%
!!	GGCTTTGAGTTTCCGAATCC+TGG	-	chr4.3:54818230-54818249	MS.gene073225:CDS	50.0%
	CCTCCAGTGCCACCAATGAA+TGG	+	chr4.3:54818131-54818150	None:intergenic	55.0%
	GGCTCAGGATCAGGATCATC+GGG	-	chr4.3:54818161-54818180	MS.gene073225:CDS	55.0%
	GGGCTCAGGATCAGGATCAT+CGG	-	chr4.3:54818160-54818179	MS.gene073225:CDS	55.0%
!	ACCCCATTCATTGGTGGCAC+TGG	-	chr4.3:54818125-54818144	MS.gene073225:CDS	55.0%
!	ATTGGTGGCACTGGAGGATC+AGG	-	chr4.3:54818134-54818153	MS.gene073225:CDS	55.0%
!	CCATTCATTGGTGGCACTGG+AGG	-	chr4.3:54818128-54818147	MS.gene073225:CDS	55.0%
!	TGGCACTGGAGGATCAGGAT+CGG	-	chr4.3:54818139-54818158	MS.gene073225:CDS	55.0%
	TCAGGATCGGGCTCAGGATC+AGG	-	chr4.3:54818152-54818171	MS.gene073225:CDS	60.0%
!	GGCACTGGAGGATCAGGATC+GGG	-	chr4.3:54818140-54818159	MS.gene073225:CDS	60.0%
	CCACCGACACCACCACTTCC+AGG	+	chr4.3:54818251-54818270	None:intergenic	65.0%
	GGAGGATCAGGATCGGGCTC+AGG	-	chr4.3:54818146-54818165	MS.gene073225:CDS	65.0%
!	CCTGGAAGTGGTGGTGTCGG+TGG	-	chr4.3:54818248-54818267	MS.gene073225:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	54817906	54818298	54817906	ID=MS.gene073225
chr4.3	mRNA	54817906	54818298	54817906	ID=MS.gene073225.t1;Parent=MS.gene073225
chr4.3	exon	54817906	54818298	54817906	ID=MS.gene073225.t1.exon1;Parent=MS.gene073225.t1
chr4.3	CDS	54817906	54818298	54817906	ID=cds.MS.gene073225.t1;Parent=MS.gene073225.t1

Gene Sequence

>MS.gene073225

ATGGTTCAAAGAGCTTACTCTTTCTTTGCACTTTTTCTCATTTTCAATATTCTCCTTCTCACAACACAAGAAGCAATTGCTGGTCGCAGCATTGCTAAGAACTCCAACAACGACGACAAGAAAGAGCCTCAGTTTCTGTTCAAGCACGAACATGGGAAACTACATTATCCAGACATTGGACATTTAGGGTTCCCACCACACTTTGGACTTACTCCTAATACCCCATTCATTGGTGGCACTGGAGGATCAGGATCGGGCTCAGGATCAGGATCATCGGGTCACAGTTATGTTCCTGGTGGCGATGACACATTTGTTCCAAACCCTGGCTTTGAGTTTCCGAATCCTGGAAGTGGTGGTGTCGGTGGTAGAGTTGCACAAACAGTTAATCCATGA

Protein sequence

>MS.gene073225.t1

MVQRAYSFFALFLIFNILLLTTQEAIAGRSIAKNSNNDDKKEPQFLFKHEHGKLHYPDIGHLGFPPHFGLTPNTPFIGGTGGSGSGSGSGSSGHSYVPGGDDTFVPNPGFEFPNPGSGGVGGRVAQTVNP