Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073641.t1 | XP_003590250.1 | 91.7 | 569 | 25 | 2 | 1 | 547 | 27 | 595 | 1.30E-271 | 945.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073641.t1 | F4I9E1 | 22.9 | 532 | 378 | 12 | 1 | 515 | 46 | 562 | 6.5e-22 | 107.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073641.t1 | G7I7D1 | 91.7 | 569 | 25 | 2 | 1 | 547 | 27 | 595 | 9.2e-272 | 945.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene073641 | 0.804404 | 2.20E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene073641 | 0.823254 | 1.53E-53 | -1.69E-46 |
| MS.gene052011 | MS.gene073641 | -0.802698 | 4.96E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene073641 | 0.808487 | 3.02E-50 | -1.69E-46 |
| MS.gene053758 | MS.gene073641 | 0.808298 | 3.32E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene073641 | 0.826089 | 3.28E-54 | -1.69E-46 |
| MS.gene055369 | MS.gene073641 | 0.81167 | 6.23E-51 | -1.69E-46 |
| MS.gene055909 | MS.gene073641 | 0.808474 | 3.04E-50 | -1.69E-46 |
| MS.gene055911 | MS.gene073641 | 0.811931 | 5.47E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene073641 | 0.80916 | 2.17E-50 | -1.69E-46 |
| MS.gene056659 | MS.gene073641 | 0.832922 | 7.17E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene073641 | 0.805961 | 1.04E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene073641 | 0.819156 | 1.35E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene073641 | 0.804447 | 2.15E-49 | -1.69E-46 |
| MS.gene058948 | MS.gene073641 | 0.811026 | 8.60E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene073641 | 0.826757 | 2.28E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073641.t1 | MTR_1g050550 | 93.497 | 569 | 15 | 3 | 1 | 547 | 27 | 595 | 0.0 | 1030 |
| MS.gene073641.t1 | MTR_1g059080 | 87.389 | 563 | 54 | 3 | 1 | 548 | 27 | 587 | 0.0 | 962 |
| MS.gene073641.t1 | MTR_1g111510 | 59.051 | 569 | 209 | 4 | 1 | 546 | 29 | 596 | 0.0 | 692 |
| MS.gene073641.t1 | MTR_1g111540 | 59.537 | 561 | 206 | 5 | 1 | 547 | 29 | 582 | 0.0 | 686 |
| MS.gene073641.t1 | MTR_1g111530 | 60.183 | 545 | 202 | 3 | 1 | 531 | 25 | 568 | 0.0 | 678 |
| MS.gene073641.t1 | MTR_1g111550 | 58.803 | 568 | 209 | 4 | 1 | 548 | 28 | 590 | 0.0 | 671 |
| MS.gene073641.t1 | MTR_7g102880 | 55.536 | 569 | 231 | 5 | 1 | 547 | 32 | 600 | 0.0 | 624 |
| MS.gene073641.t1 | MTR_2g100400 | 34.601 | 552 | 315 | 9 | 2 | 528 | 11 | 541 | 7.90e-106 | 328 |
| MS.gene073641.t1 | MTR_5g090570 | 35.327 | 535 | 315 | 6 | 4 | 525 | 17 | 533 | 5.11e-103 | 320 |
| MS.gene073641.t1 | MTR_7g029015 | 35.563 | 568 | 303 | 14 | 8 | 522 | 25 | 582 | 1.31e-96 | 305 |
| MS.gene073641.t1 | MTR_3g099150 | 34.270 | 534 | 320 | 8 | 9 | 516 | 26 | 554 | 1.21e-91 | 292 |
| MS.gene073641.t1 | MTR_4g073690 | 35.038 | 528 | 314 | 7 | 4 | 516 | 21 | 534 | 1.32e-86 | 278 |
| MS.gene073641.t1 | MTR_5g022380 | 32.169 | 544 | 330 | 9 | 4 | 535 | 19 | 535 | 3.01e-86 | 277 |
| MS.gene073641.t1 | MTR_2g450710 | 32.276 | 536 | 324 | 9 | 12 | 516 | 26 | 553 | 3.61e-80 | 262 |
| MS.gene073641.t1 | MTR_4g116210 | 30.769 | 546 | 324 | 10 | 13 | 516 | 26 | 559 | 2.50e-78 | 257 |
| MS.gene073641.t1 | MTR_5g022380 | 31.416 | 452 | 271 | 9 | 96 | 535 | 1 | 425 | 1.57e-64 | 217 |
| MS.gene073641.t1 | MTR_4g073690 | 33.862 | 378 | 230 | 6 | 4 | 366 | 21 | 393 | 1.58e-52 | 184 |
| MS.gene073641.t1 | MTR_3g466040 | 27.273 | 528 | 332 | 12 | 8 | 515 | 17 | 512 | 1.80e-36 | 142 |
| MS.gene073641.t1 | MTR_3g466110 | 25.673 | 483 | 325 | 8 | 8 | 473 | 17 | 482 | 1.67e-34 | 136 |
| MS.gene073641.t1 | MTR_7g083660 | 25.352 | 568 | 370 | 14 | 1 | 530 | 10 | 561 | 1.94e-30 | 125 |
| MS.gene073641.t1 | MTR_2g104250 | 26.848 | 257 | 140 | 6 | 260 | 516 | 81 | 289 | 9.46e-26 | 108 |
| MS.gene073641.t1 | MTR_5g026350 | 25.214 | 583 | 358 | 21 | 1 | 528 | 10 | 569 | 1.78e-24 | 107 |
| MS.gene073641.t1 | MTR_5g095240 | 60.563 | 71 | 28 | 0 | 452 | 522 | 2 | 72 | 2.21e-23 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073641.t1 | AT2G39210 | 60.589 | 543 | 197 | 2 | 1 | 526 | 24 | 566 | 0.0 | 687 |
| MS.gene073641.t1 | AT2G28120 | 58.152 | 552 | 218 | 3 | 1 | 540 | 20 | 570 | 0.0 | 655 |
| MS.gene073641.t1 | AT1G74780 | 37.106 | 539 | 305 | 5 | 4 | 526 | 13 | 533 | 2.39e-114 | 350 |
| MS.gene073641.t1 | AT5G50520 | 35.385 | 520 | 324 | 6 | 1 | 515 | 18 | 530 | 1.41e-103 | 322 |
| MS.gene073641.t1 | AT5G50630 | 35.385 | 520 | 324 | 6 | 1 | 515 | 18 | 530 | 1.41e-103 | 322 |
| MS.gene073641.t1 | AT1G18940 | 36.023 | 533 | 301 | 6 | 4 | 522 | 15 | 521 | 5.71e-103 | 320 |
| MS.gene073641.t1 | AT2G34350 | 34.652 | 531 | 314 | 6 | 4 | 522 | 13 | 522 | 1.24e-101 | 317 |
| MS.gene073641.t1 | AT2G34350 | 34.652 | 531 | 314 | 6 | 4 | 522 | 13 | 522 | 1.24e-101 | 317 |
| MS.gene073641.t1 | AT2G34355 | 34.522 | 533 | 310 | 7 | 4 | 522 | 13 | 520 | 6.91e-99 | 309 |
| MS.gene073641.t1 | AT5G14120 | 34.151 | 571 | 319 | 12 | 1 | 524 | 18 | 578 | 2.15e-98 | 310 |
| MS.gene073641.t1 | AT3G01930 | 32.404 | 574 | 330 | 9 | 1 | 524 | 18 | 583 | 1.26e-93 | 298 |
| MS.gene073641.t1 | AT2G16660 | 35.182 | 523 | 309 | 6 | 8 | 516 | 24 | 530 | 1.02e-92 | 294 |
| MS.gene073641.t1 | AT4G34950 | 34.342 | 562 | 307 | 12 | 8 | 529 | 26 | 565 | 4.94e-91 | 290 |
| MS.gene073641.t1 | AT1G80530 | 30.168 | 537 | 326 | 10 | 12 | 517 | 27 | 545 | 8.95e-74 | 245 |
| MS.gene073641.t1 | AT3G01930 | 30.544 | 478 | 275 | 8 | 96 | 524 | 1 | 470 | 2.54e-66 | 223 |
| MS.gene073641.t1 | AT2G34355 | 32.741 | 394 | 234 | 6 | 135 | 522 | 2 | 370 | 2.38e-64 | 214 |
| MS.gene073641.t1 | AT3G01930 | 31.442 | 423 | 235 | 7 | 1 | 373 | 18 | 435 | 1.71e-57 | 198 |
| MS.gene073641.t1 | AT2G16660 | 33.780 | 373 | 226 | 5 | 8 | 366 | 24 | 389 | 7.06e-54 | 187 |
| MS.gene073641.t1 | AT3G01630 | 25.234 | 535 | 359 | 16 | 4 | 516 | 47 | 562 | 1.32e-30 | 126 |
| MS.gene073641.t1 | AT3G01630 | 25.234 | 535 | 359 | 16 | 4 | 516 | 29 | 544 | 1.85e-30 | 125 |
| MS.gene073641.t1 | AT4G19450 | 26.587 | 583 | 353 | 18 | 1 | 532 | 10 | 568 | 5.15e-30 | 124 |
| MS.gene073641.t1 | AT2G30300 | 25.143 | 525 | 331 | 13 | 8 | 515 | 17 | 496 | 4.63e-28 | 118 |
| MS.gene073641.t1 | AT1G31470 | 24.812 | 532 | 364 | 14 | 4 | 516 | 49 | 563 | 5.40e-26 | 112 |
| MS.gene073641.t1 | AT4G19450 | 25.369 | 406 | 242 | 12 | 1 | 356 | 10 | 404 | 1.57e-19 | 91.7 |
| MS.gene073641.t1 | AT5G45275 | 31.053 | 190 | 129 | 2 | 1 | 189 | 10 | 198 | 1.69e-16 | 83.2 |
Find 124 sgRNAs with CRISPR-Local
Find 136 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCTTGTTGTTGTTATTA+TGG | 0.134860 | 1.1:-34999999 | MS.gene073641:CDS |
| ATCACTGCTGTTAGCCTCTT+TGG | 0.157307 | 1.1:-34999233 | MS.gene073641:CDS |
| TGTCTTTAACTTTGGTTATA+AGG | 0.204092 | 1.1:-34999202 | MS.gene073641:CDS |
| CTAATGCTGCCATTTGCTTT+AGG | 0.212154 | 1.1:+34999327 | None:intergenic |
| AAGAGAAATCAAATCAGTTT+TGG | 0.212226 | 1.1:-35000644 | MS.gene073641:CDS |
| ATATTCCATATTGGAGGTTT+AGG | 0.223435 | 1.1:+35000445 | None:intergenic |
| TAATGCTGCCATTTGCTTTA+GGG | 0.225798 | 1.1:+34999328 | None:intergenic |
| TACATGTCTTTAATCCTTGC+AGG | 0.243168 | 1.1:-35000118 | MS.gene073641:CDS |
| GTAAATAACTTGAGTCAAAT+TGG | 0.246330 | 1.1:-34999743 | MS.gene073641:CDS |
| CTTGAGCTTCTTCAAGGACT+TGG | 0.257995 | 1.1:-35000596 | MS.gene073641:CDS |
| AGTCAAATTGGATTATCTTT+AGG | 0.269385 | 1.1:-34999731 | MS.gene073641:CDS |
| TTGTGTCCCTAATGGCAATT+TGG | 0.273307 | 1.1:-34999682 | MS.gene073641:CDS |
| CAACTATACTATGCCTTCTA+TGG | 0.279543 | 1.1:-35000274 | MS.gene073641:CDS |
| ATCCTTATATTGTCTTGTTT+TGG | 0.280340 | 1.1:-34999572 | MS.gene073641:CDS |
| TCACTGCTGTTAGCCTCTTT+GGG | 0.282953 | 1.1:-34999232 | MS.gene073641:CDS |
| TATTCCATATTGGAGGTTTA+GGG | 0.301426 | 1.1:+35000446 | None:intergenic |
| TTGGTTGTTCAGCTCTCTTA+TGG | 0.310124 | 1.1:+35000170 | None:intergenic |
| GACCAAAACAAGACAATATA+AGG | 0.312581 | 1.1:+34999570 | None:intergenic |
| CACTCTCTTGAGCTTCTTCA+AGG | 0.315653 | 1.1:-35000602 | MS.gene073641:CDS |
| TCAATCATATCTGAACTCTT+TGG | 0.320938 | 1.1:-34999455 | MS.gene073641:CDS |
| TTTGGAGTCATTTCCATAGA+AGG | 0.324553 | 1.1:+35000261 | None:intergenic |
| GTTTCTGCTTTGGTGCAAAT+TGG | 0.326521 | 1.1:-34999490 | MS.gene073641:CDS |
| CATAAGCAAAATTAAAGATT+TGG | 0.327529 | 1.1:+35000243 | None:intergenic |
| ATTATTGTTGGTTTCTGCTT+TGG | 0.335466 | 1.1:-34999500 | MS.gene073641:CDS |
| CTAAAGCAAATGGCAGCATT+AGG | 0.335582 | 1.1:-34999326 | MS.gene073641:CDS |
| CATCATGTCAAGGCTGAAAA+TGG | 0.337307 | 1.1:+34999814 | None:intergenic |
| CCTTGGGTTGTCTTAACAAT+TGG | 0.343987 | 1.1:-35000526 | MS.gene073641:CDS |
| TTTATGATCATGTCAGTATC+TGG | 0.347170 | 1.1:-35000694 | MS.gene073641:CDS |
| TTGTTAAGTGTGAGGGTTGC+TGG | 0.349828 | 1.1:-34999371 | MS.gene073641:CDS |
| GATAGATCCAAATTGCCATT+AGG | 0.350724 | 1.1:+34999675 | None:intergenic |
| CTCTTATGGTGCTTAATAAC+TGG | 0.351299 | 1.1:+35000184 | None:intergenic |
| TTATGGAAGAACAAAGGATT+TGG | 0.359352 | 1.1:-34999982 | MS.gene073641:CDS |
| GGCACTTGTGTCTTTAACTT+TGG | 0.360219 | 1.1:-34999210 | MS.gene073641:CDS |
| GAACATCAAATGCAATTAGA+AGG | 0.360477 | 1.1:+34999546 | None:intergenic |
| TATGTAGCATCAATTATTGT+TGG | 0.362356 | 1.1:-34999512 | MS.gene073641:CDS |
| AAATCCCTAAACCTCCAATA+TGG | 0.365760 | 1.1:-35000450 | MS.gene073641:CDS |
| TGGTGAGTCTGTGGCTTAGA+AGG | 0.373922 | 1.1:+34999923 | None:intergenic |
| TAGAAATAATGAAAATGAAT+TGG | 0.376045 | 1.1:-34999129 | MS.gene073641:CDS |
| GGGTCAATAGCAAGTCCAAT+AGG | 0.385410 | 1.1:-34999401 | MS.gene073641:CDS |
| TGGAAGTCAGTGCTACAAAT+TGG | 0.387074 | 1.1:-34999267 | MS.gene073641:CDS |
| GAGCAAGAGAAGCATCAAAA+TGG | 0.387481 | 1.1:+35000027 | None:intergenic |
| ATAGATCCAAATTGCCATTA+GGG | 0.390342 | 1.1:+34999676 | None:intergenic |
| GTGGGCACAATTTGTGGACT+TGG | 0.397228 | 1.1:-34999782 | MS.gene073641:CDS |
| TGGGTTGTCTTAACAATTGG+TGG | 0.401882 | 1.1:-35000523 | MS.gene073641:CDS |
| GATGCTACATAGAGACCATT+TGG | 0.414023 | 1.1:+34999524 | None:intergenic |
| CTTAACAAGTATGACCCTAT+TGG | 0.415970 | 1.1:+34999386 | None:intergenic |
| TATAAGCACATATTCCATAT+TGG | 0.421719 | 1.1:+35000436 | None:intergenic |
| GTATGATCTTCACCTCTACT+TGG | 0.422261 | 1.1:+34999848 | None:intergenic |
| TACTCAACATTGTATAATGT+TGG | 0.424470 | 1.1:-34999422 | MS.gene073641:CDS |
| CAATGAAATCACACCTCCTT+GGG | 0.429265 | 1.1:-35000542 | MS.gene073641:CDS |
| ATGGCAATTTGGATCTATCT+AGG | 0.429608 | 1.1:-34999671 | MS.gene073641:CDS |
| ATGTGCTTATACATATTCAT+TGG | 0.430843 | 1.1:-35000424 | MS.gene073641:CDS |
| GAGTAGTCTCTCCTCTAGAT+TGG | 0.434002 | 1.1:+34999903 | None:intergenic |
| TTGAGCTTCTTCAAGGACTT+GGG | 0.443522 | 1.1:-35000595 | MS.gene073641:CDS |
| TCTGGAGCAAGCTACATGTT+TGG | 0.448168 | 1.1:-35000676 | MS.gene073641:CDS |
| ACTCAACATTGTATAATGTT+GGG | 0.455480 | 1.1:-34999421 | MS.gene073641:CDS |
| TTGCAATTCAACTCCTCCCC+TGG | 0.457387 | 1.1:+34999290 | None:intergenic |
| GGTAGTAGAGGTGTTGTCAT+AGG | 0.459692 | 1.1:-35000340 | MS.gene073641:CDS |
| ATTGCATTTGATGTTCCAAA+TGG | 0.463625 | 1.1:-34999539 | MS.gene073641:CDS |
| GATGTGAGCATGAGAGGCCT+TGG | 0.466925 | 1.1:+34999596 | None:intergenic |
| TTAGGGTTAAAGAGGAAGCC+AGG | 0.467861 | 1.1:-34999308 | MS.gene073641:CDS |
| TAAAGCAAATGGCAGCATTA+GGG | 0.468002 | 1.1:-34999325 | MS.gene073641:CDS |
| GTTGGTTGAACAATGAGAAT+TGG | 0.470515 | 1.1:+35000399 | None:intergenic |
| TCAATGAAATCACACCTCCT+TGG | 0.473514 | 1.1:-35000543 | MS.gene073641:CDS |
| TATGATCTTCACCTCTACTT+GGG | 0.477700 | 1.1:+34999849 | None:intergenic |
| AACCACATTTGTGTCCCTAA+TGG | 0.479854 | 1.1:-34999690 | MS.gene073641:CDS |
| TTCACCTCTACTTGGGGAAG+AGG | 0.481674 | 1.1:+34999856 | None:intergenic |
| TAAAGAAGCCCTAAAGCAAA+TGG | 0.492245 | 1.1:-34999336 | MS.gene073641:CDS |
| ATCTGTCTATGCATCATTTG+AGG | 0.496384 | 1.1:+34999079 | None:intergenic |
| ACATGTCTTTAATCCTTGCA+GGG | 0.496948 | 1.1:-35000117 | MS.gene073641:CDS |
| CACTGCTGTTAGCCTCTTTG+GGG | 0.499507 | 1.1:-34999231 | MS.gene073641:CDS |
| GTCATACTTGTTAAGTGTGA+GGG | 0.503976 | 1.1:-34999378 | MS.gene073641:CDS |
| TGGCAGCATTAGGGTTAAAG+AGG | 0.518733 | 1.1:-34999316 | MS.gene073641:CDS |
| AAGCACATATTCCATATTGG+AGG | 0.518894 | 1.1:+35000439 | None:intergenic |
| GTCTATGCATCATTTGAGGC+AGG | 0.522205 | 1.1:+34999083 | None:intergenic |
| AGTGGTTATCTTGGTTTGAG+TGG | 0.525713 | 1.1:-35000310 | MS.gene073641:CDS |
| AAAACAAAAGTTACAGCAGT+TGG | 0.525937 | 1.1:+35000211 | None:intergenic |
| GATATGATTGAAAATAGTAC+TGG | 0.527060 | 1.1:+34999467 | None:intergenic |
| AACCAAGATAACCACTCAAA+AGG | 0.532585 | 1.1:+35000317 | None:intergenic |
| TAGGGTTAAAGAGGAAGCCA+GGG | 0.536413 | 1.1:-34999307 | MS.gene073641:CDS |
| GGCACAATTTGTGGACTTGG+TGG | 0.540472 | 1.1:-34999779 | MS.gene073641:CDS |
| AGGATTGATGTGAGCATGAG+AGG | 0.546350 | 1.1:+34999590 | None:intergenic |
| CCTCTAGATTGGTGAGTCTG+TGG | 0.548972 | 1.1:+34999914 | None:intergenic |
| TCCATAATAACAACAACAAG+AGG | 0.549935 | 1.1:+34999998 | None:intergenic |
| GGTCAATAGCAAGTCCAATA+GGG | 0.554065 | 1.1:-34999400 | MS.gene073641:CDS |
| GTTGTGACTCAGAACAAGGT+TGG | 0.560023 | 1.1:-34999107 | MS.gene073641:CDS |
| AAGAATTTCCCTGGTAGTAG+AGG | 0.560197 | 1.1:-35000352 | MS.gene073641:CDS |
| TCTAACATAGGCATTCTCTC+AGG | 0.560326 | 1.1:-35000571 | MS.gene073641:CDS |
| GAAGCTCAAGAGAGTGAGAG+TGG | 0.562224 | 1.1:+35000609 | None:intergenic |
| GGGGAGGAGTTGAATTGCAA+TGG | 0.565523 | 1.1:-34999287 | MS.gene073641:CDS |
| ATGACAACACCTCTACTACC+AGG | 0.567626 | 1.1:+35000343 | None:intergenic |
| ACCAGTGTAAAGAATTTCCC+TGG | 0.568154 | 1.1:-35000361 | MS.gene073641:CDS |
| TCAAGAGAGTGAGAGTGGAT+TGG | 0.568198 | 1.1:+35000614 | None:intergenic |
| TCTCATTGTTCAACCAACAC+TGG | 0.569882 | 1.1:-35000394 | MS.gene073641:CDS |
| AATGTGGTTATACTATGTGC+AGG | 0.572229 | 1.1:+34999704 | None:intergenic |
| AAGGACTTGGGATCTAACAT+AGG | 0.575857 | 1.1:-35000583 | MS.gene073641:CDS |
| TGCCATTAGGGACACAAATG+TGG | 0.577289 | 1.1:+34999688 | None:intergenic |
| ATGTGAGCATGAGAGGCCTT+GGG | 0.579736 | 1.1:+34999597 | None:intergenic |
| ACCAGGGAAATTCTTTACAC+TGG | 0.584117 | 1.1:+35000360 | None:intergenic |
| TTGTTATTATGGAAGAACAA+AGG | 0.585080 | 1.1:-34999988 | MS.gene073641:CDS |
| GGTCATACTTGTTAAGTGTG+AGG | 0.586817 | 1.1:-34999379 | MS.gene073641:CDS |
| GCACTATGATCATTATCATG+AGG | 0.591440 | 1.1:+35000092 | None:intergenic |
| GTTTCCTCTTCCCCAAGTAG+AGG | 0.594548 | 1.1:-34999860 | MS.gene073641:CDS |
| ATGATCTTCACCTCTACTTG+GGG | 0.609933 | 1.1:+34999850 | None:intergenic |
| TAACAAAGTTAAAACTCCCA+AGG | 0.611560 | 1.1:-34999613 | MS.gene073641:CDS |
| TTATCATGAGGAACCCTGCA+AGG | 0.614007 | 1.1:+35000104 | None:intergenic |
| AAGACACAAGTGCCCCAAAG+AGG | 0.618650 | 1.1:+34999219 | None:intergenic |
| CCAATTGTTAAGACAACCCA+AGG | 0.620677 | 1.1:+35000526 | None:intergenic |
| GTTAAAGAGGAAGCCAGGGG+AGG | 0.622388 | 1.1:-34999303 | MS.gene073641:CDS |
| GAGGTGTGATTTCATTGAGT+AGG | 0.632454 | 1.1:+35000548 | None:intergenic |
| TGACAACACCTCTACTACCA+GGG | 0.633964 | 1.1:+35000344 | None:intergenic |
| ATCTAGGTAAAGTTACACAA+GGG | 0.638121 | 1.1:-34999655 | MS.gene073641:CDS |
| TATCTAGGTAAAGTTACACA+AGG | 0.663423 | 1.1:-34999656 | MS.gene073641:CDS |
| CCATAATAACAACAACAAGA+GGG | 0.664165 | 1.1:+34999999 | None:intergenic |
| AGGGTTAAAGAGGAAGCCAG+GGG | 0.664886 | 1.1:-34999306 | MS.gene073641:CDS |
| ACTTGGTGGAACTCTTACTG+TGG | 0.667152 | 1.1:-34999765 | MS.gene073641:CDS |
| TGAGTCTGTGGCTTAGAAGG+AGG | 0.667868 | 1.1:+34999926 | None:intergenic |
| GGTGACTAAAGCTCCAGTGT+TGG | 0.668900 | 1.1:+35000381 | None:intergenic |
| ATTGGTTGTGACTCAGAACA+AGG | 0.669577 | 1.1:-34999111 | MS.gene073641:CDS |
| TCTAGGTAAAGTTACACAAG+GGG | 0.677668 | 1.1:-34999654 | MS.gene073641:CDS |
| CCACAGACTCACCAATCTAG+AGG | 0.680979 | 1.1:-34999914 | MS.gene073641:CDS |
| TAATAACAACAACAAGAGGG+AGG | 0.686207 | 1.1:+35000002 | None:intergenic |
| CATATACAAGAAGTTCAGAG+AGG | 0.708025 | 1.1:-34999156 | MS.gene073641:CDS |
| ATTGTTAAGACAACCCAAGG+AGG | 0.744267 | 1.1:+35000529 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAATTATAAAAAGCTT+TGG | + | chr1.1:34999656-34999675 | None:intergenic | 15.0% |
| !! | TAGAAATAATGAAAATGAAT+TGG | - | chr1.1:35000665-35000684 | MS.gene073641:CDS | 15.0% |
| !! | CATAAGCAAAATTAAAGATT+TGG | + | chr1.1:34999554-34999573 | None:intergenic | 20.0% |
| !!! | CAAATCTTTAATTTTGCTTA+TGG | - | chr1.1:34999552-34999571 | MS.gene073641:CDS | 20.0% |
| ! | ACTCAACATTGTATAATGTT+GGG | - | chr1.1:35000373-35000392 | MS.gene073641:CDS | 25.0% |
| ! | AGTCAAATTGGATTATCTTT+AGG | - | chr1.1:35000063-35000082 | MS.gene073641:CDS | 25.0% |
| ! | ATGTGCTTATACATATTCAT+TGG | - | chr1.1:34999370-34999389 | MS.gene073641:CDS | 25.0% |
| ! | GATATGATTGAAAATAGTAC+TGG | + | chr1.1:35000330-35000349 | None:intergenic | 25.0% |
| ! | GTAAATAACTTGAGTCAAAT+TGG | - | chr1.1:35000051-35000070 | MS.gene073641:CDS | 25.0% |
| ! | TACTCAACATTGTATAATGT+TGG | - | chr1.1:35000372-35000391 | MS.gene073641:CDS | 25.0% |
| ! | TATAAGCACATATTCCATAT+TGG | + | chr1.1:34999361-34999380 | None:intergenic | 25.0% |
| ! | TATGTAGCATCAATTATTGT+TGG | - | chr1.1:35000282-35000301 | MS.gene073641:CDS | 25.0% |
| !! | AAGAGAAATCAAATCAGTTT+TGG | - | chr1.1:34999150-34999169 | MS.gene073641:CDS | 25.0% |
| !! | AGAGAAATCAAATCAGTTTT+GGG | - | chr1.1:34999151-34999170 | MS.gene073641:CDS | 25.0% |
| !! | TGTCTTTAACTTTGGTTATA+AGG | - | chr1.1:35000592-35000611 | MS.gene073641:CDS | 25.0% |
| !! | TTGTTATTATGGAAGAACAA+AGG | - | chr1.1:34999806-34999825 | MS.gene073641:CDS | 25.0% |
| !!! | ATCCTTATATTGTCTTGTTT+TGG | - | chr1.1:35000222-35000241 | MS.gene073641:CDS | 25.0% |
| !!! | TAAAAAGCTTTGGAATCATT+TGG | + | chr1.1:34999646-34999665 | None:intergenic | 25.0% |
| !!! | TTTTTGGCTATTTCATGATT+TGG | - | chr1.1:34999305-34999324 | MS.gene073641:CDS | 25.0% |
| AAAACAAAAGTTACAGCAGT+TGG | + | chr1.1:34999586-34999605 | None:intergenic | 30.0% | |
| ATAGATCCAAATTGCCATTA+GGG | + | chr1.1:35000121-35000140 | None:intergenic | 30.0% | |
| ATATTCCATATTGGAGGTTT+AGG | + | chr1.1:34999352-34999371 | None:intergenic | 30.0% | |
| ATCTAGGTAAAGTTACACAA+GGG | - | chr1.1:35000139-35000158 | MS.gene073641:CDS | 30.0% | |
| ATTGCATTTGATGTTCCAAA+TGG | - | chr1.1:35000255-35000274 | MS.gene073641:CDS | 30.0% | |
| CCATAATAACAACAACAAGA+GGG | + | chr1.1:34999798-34999817 | None:intergenic | 30.0% | |
| GAACATCAAATGCAATTAGA+AGG | + | chr1.1:35000251-35000270 | None:intergenic | 30.0% | |
| GACCAAAACAAGACAATATA+AGG | + | chr1.1:35000227-35000246 | None:intergenic | 30.0% | |
| TAACAAAGTTAAAACTCCCA+AGG | - | chr1.1:35000181-35000200 | MS.gene073641:CDS | 30.0% | |
| TATCTAGGTAAAGTTACACA+AGG | - | chr1.1:35000138-35000157 | MS.gene073641:CDS | 30.0% | |
| TATTCCATATTGGAGGTTTA+GGG | + | chr1.1:34999351-34999370 | None:intergenic | 30.0% | |
| TCAATCATATCTGAACTCTT+TGG | - | chr1.1:35000339-35000358 | MS.gene073641:CDS | 30.0% | |
| TCCATAATAACAACAACAAG+AGG | + | chr1.1:34999799-34999818 | None:intergenic | 30.0% | |
| TTATGGAAGAACAAAGGATT+TGG | - | chr1.1:34999812-34999831 | MS.gene073641:CDS | 30.0% | |
| TTTATGATCATGTCAGTATC+TGG | - | chr1.1:34999100-34999119 | MS.gene073641:CDS | 30.0% | |
| !! | AGGACTAGAGAGTTTTATAA+AGG | - | chr1.1:35000612-35000631 | MS.gene073641:CDS | 30.0% |
| !! | ATTATTGTTGGTTTCTGCTT+TGG | - | chr1.1:35000294-35000313 | MS.gene073641:CDS | 30.0% |
| !! | CTTTAATTTTGCTTATGGCT+TGG | - | chr1.1:34999557-34999576 | MS.gene073641:CDS | 30.0% |
| AAATCCCTAAACCTCCAATA+TGG | - | chr1.1:34999344-34999363 | MS.gene073641:CDS | 35.0% | |
| AACCAAGATAACCACTCAAA+AGG | + | chr1.1:34999480-34999499 | None:intergenic | 35.0% | |
| AAGCACATATTCCATATTGG+AGG | + | chr1.1:34999358-34999377 | None:intergenic | 35.0% | |
| AATGTGGTTATACTATGTGC+AGG | + | chr1.1:35000093-35000112 | None:intergenic | 35.0% | |
| ACATGTCTTTAATCCTTGCA+GGG | - | chr1.1:34999677-34999696 | MS.gene073641:CDS | 35.0% | |
| ATGGCAATTTGGATCTATCT+AGG | - | chr1.1:35000123-35000142 | MS.gene073641:CDS | 35.0% | |
| CAAAAAGAGTCATCATGTCA+AGG | + | chr1.1:34999993-35000012 | None:intergenic | 35.0% | |
| CAACTATACTATGCCTTCTA+TGG | - | chr1.1:34999520-34999539 | MS.gene073641:CDS | 35.0% | |
| CATATACAAGAAGTTCAGAG+AGG | - | chr1.1:35000638-35000657 | MS.gene073641:CDS | 35.0% | |
| CTCTTATGGTGCTTAATAAC+TGG | + | chr1.1:34999613-34999632 | None:intergenic | 35.0% | |
| CTTAACAAGTATGACCCTAT+TGG | + | chr1.1:35000411-35000430 | None:intergenic | 35.0% | |
| GATAGATCCAAATTGCCATT+AGG | + | chr1.1:35000122-35000141 | None:intergenic | 35.0% | |
| GCACTATGATCATTATCATG+AGG | + | chr1.1:34999705-34999724 | None:intergenic | 35.0% | |
| GTTGGTTGAACAATGAGAAT+TGG | + | chr1.1:34999398-34999417 | None:intergenic | 35.0% | |
| TAAAGAAGCCCTAAAGCAAA+TGG | - | chr1.1:35000458-35000477 | MS.gene073641:CDS | 35.0% | |
| TAAAGCAAATGGCAGCATTA+GGG | - | chr1.1:35000469-35000488 | MS.gene073641:CDS | 35.0% | |
| TAATAACAACAACAAGAGGG+AGG | + | chr1.1:34999795-34999814 | None:intergenic | 35.0% | |
| TACATGTCTTTAATCCTTGC+AGG | - | chr1.1:34999676-34999695 | MS.gene073641:CDS | 35.0% | |
| TATGATCTTCACCTCTACTT+GGG | + | chr1.1:34999948-34999967 | None:intergenic | 35.0% | |
| TCTAGGTAAAGTTACACAAG+GGG | - | chr1.1:35000140-35000159 | MS.gene073641:CDS | 35.0% | |
| ! | GACATGATGACTCTTTTTGT+GGG | - | chr1.1:34999994-35000013 | MS.gene073641:CDS | 35.0% |
| ! | GTCATACTTGTTAAGTGTGA+GGG | - | chr1.1:35000416-35000435 | MS.gene073641:CDS | 35.0% |
| ! | TAATGCTGCCATTTGCTTTA+GGG | + | chr1.1:35000469-35000488 | None:intergenic | 35.0% |
| ! | TGACATGATGACTCTTTTTG+TGG | - | chr1.1:34999993-35000012 | MS.gene073641:CDS | 35.0% |
| ! | TTTGGAGTCATTTCCATAGA+AGG | + | chr1.1:34999536-34999555 | None:intergenic | 35.0% |
| !! | CCCTCTTGTTGTTGTTATTA+TGG | - | chr1.1:34999795-34999814 | MS.gene073641:CDS | 35.0% |
| AACCACATTTGTGTCCCTAA+TGG | - | chr1.1:35000104-35000123 | MS.gene073641:CDS | 40.0% | |
| AAGAATTTCCCTGGTAGTAG+AGG | - | chr1.1:34999442-34999461 | MS.gene073641:CDS | 40.0% | |
| AAGGACTTGGGATCTAACAT+AGG | - | chr1.1:34999211-34999230 | MS.gene073641:CDS | 40.0% | |
| ACCAGGGAAATTCTTTACAC+TGG | + | chr1.1:34999437-34999456 | None:intergenic | 40.0% | |
| ACCAGTGTAAAGAATTTCCC+TGG | - | chr1.1:34999433-34999452 | MS.gene073641:CDS | 40.0% | |
| ATGATCTTCACCTCTACTTG+GGG | + | chr1.1:34999947-34999966 | None:intergenic | 40.0% | |
| ATTGGTTGTGACTCAGAACA+AGG | - | chr1.1:35000683-35000702 | MS.gene073641:CDS | 40.0% | |
| ATTGTTAAGACAACCCAAGG+AGG | + | chr1.1:34999268-34999287 | None:intergenic | 40.0% | |
| CAATGAAATCACACCTCCTT+GGG | - | chr1.1:34999252-34999271 | MS.gene073641:CDS | 40.0% | |
| CATCATGTCAAGGCTGAAAA+TGG | + | chr1.1:34999983-35000002 | None:intergenic | 40.0% | |
| CCAATTGTTAAGACAACCCA+AGG | + | chr1.1:34999271-34999290 | None:intergenic | 40.0% | |
| CTAAAGCAAATGGCAGCATT+AGG | - | chr1.1:35000468-35000487 | MS.gene073641:CDS | 40.0% | |
| GAGGTGTGATTTCATTGAGT+AGG | + | chr1.1:34999249-34999268 | None:intergenic | 40.0% | |
| GATGCTACATAGAGACCATT+TGG | + | chr1.1:35000273-35000292 | None:intergenic | 40.0% | |
| GGTCAATAGCAAGTCCAATA+GGG | - | chr1.1:35000394-35000413 | MS.gene073641:CDS | 40.0% | |
| GTATGATCTTCACCTCTACT+TGG | + | chr1.1:34999949-34999968 | None:intergenic | 40.0% | |
| TCAATGAAATCACACCTCCT+TGG | - | chr1.1:34999251-34999270 | MS.gene073641:CDS | 40.0% | |
| TCTAACATAGGCATTCTCTC+AGG | - | chr1.1:34999223-34999242 | MS.gene073641:CDS | 40.0% | |
| TCTCATTGTTCAACCAACAC+TGG | - | chr1.1:34999400-34999419 | MS.gene073641:CDS | 40.0% | |
| TGGAAGTCAGTGCTACAAAT+TGG | - | chr1.1:35000527-35000546 | MS.gene073641:CDS | 40.0% | |
| TGGGTTGTCTTAACAATTGG+TGG | - | chr1.1:34999271-34999290 | MS.gene073641:CDS | 40.0% | |
| TTGAGCTTCTTCAAGGACTT+GGG | - | chr1.1:34999199-34999218 | MS.gene073641:CDS | 40.0% | |
| TTGGTTGTTCAGCTCTCTTA+TGG | + | chr1.1:34999627-34999646 | None:intergenic | 40.0% | |
| TTGTGTCCCTAATGGCAATT+TGG | - | chr1.1:35000112-35000131 | MS.gene073641:CDS | 40.0% | |
| ! | AGTGGTTATCTTGGTTTGAG+TGG | - | chr1.1:34999484-34999503 | MS.gene073641:CDS | 40.0% |
| ! | CCTTGGGTTGTCTTAACAAT+TGG | - | chr1.1:34999268-34999287 | MS.gene073641:CDS | 40.0% |
| ! | CTAATGCTGCCATTTGCTTT+AGG | + | chr1.1:35000470-35000489 | None:intergenic | 40.0% |
| ! | CTTTTTGTGGGCACAATTTG+TGG | - | chr1.1:35000006-35000025 | MS.gene073641:CDS | 40.0% |
| ! | GGCACTTGTGTCTTTAACTT+TGG | - | chr1.1:35000584-35000603 | MS.gene073641:CDS | 40.0% |
| ! | GGTCATACTTGTTAAGTGTG+AGG | - | chr1.1:35000415-35000434 | MS.gene073641:CDS | 40.0% |
| ! | GTTTCTGCTTTGGTGCAAAT+TGG | - | chr1.1:35000304-35000323 | MS.gene073641:CDS | 40.0% |
| !! | GAGCAAGAGAAGCATCAAAA+TGG | + | chr1.1:34999770-34999789 | None:intergenic | 40.0% |
| !!! | GGTGGTCTTCTCAACTTTTT+TGG | - | chr1.1:34999289-34999308 | MS.gene073641:CDS | 40.0% |
| AGGATTGATGTGAGCATGAG+AGG | + | chr1.1:35000207-35000226 | None:intergenic | 45.0% | |
| ATGACAACACCTCTACTACC+AGG | + | chr1.1:34999454-34999473 | None:intergenic | 45.0% | |
| CACTCTCTTGAGCTTCTTCA+AGG | - | chr1.1:34999192-34999211 | MS.gene073641:CDS | 45.0% | |
| CTTGAGCTTCTTCAAGGACT+TGG | - | chr1.1:34999198-34999217 | MS.gene073641:CDS | 45.0% | |
| GAGTAGTCTCTCCTCTAGAT+TGG | + | chr1.1:34999894-34999913 | None:intergenic | 45.0% | |
| GGGTCAATAGCAAGTCCAAT+AGG | - | chr1.1:35000393-35000412 | MS.gene073641:CDS | 45.0% | |
| GTTGTGACTCAGAACAAGGT+TGG | - | chr1.1:35000687-35000706 | MS.gene073641:CDS | 45.0% | |
| TAGGGTTAAAGAGGAAGCCA+GGG | - | chr1.1:35000487-35000506 | MS.gene073641:CDS | 45.0% | |
| TCAAGAGAGTGAGAGTGGAT+TGG | + | chr1.1:34999183-34999202 | None:intergenic | 45.0% | |
| TGACAACACCTCTACTACCA+GGG | + | chr1.1:34999453-34999472 | None:intergenic | 45.0% | |
| TGCCATTAGGGACACAAATG+TGG | + | chr1.1:35000109-35000128 | None:intergenic | 45.0% | |
| TGGCAGCATTAGGGTTAAAG+AGG | - | chr1.1:35000478-35000497 | MS.gene073641:CDS | 45.0% | |
| TTAGGGTTAAAGAGGAAGCC+AGG | - | chr1.1:35000486-35000505 | MS.gene073641:CDS | 45.0% | |
| TTATCATGAGGAACCCTGCA+AGG | + | chr1.1:34999693-34999712 | None:intergenic | 45.0% | |
| TTGTTAAGTGTGAGGGTTGC+TGG | - | chr1.1:35000423-35000442 | MS.gene073641:CDS | 45.0% | |
| ! | ACTTGGTGGAACTCTTACTG+TGG | - | chr1.1:35000029-35000048 | MS.gene073641:CDS | 45.0% |
| ! | ATCACTGCTGTTAGCCTCTT+TGG | - | chr1.1:35000561-35000580 | MS.gene073641:CDS | 45.0% |
| ! | GTTGTCATAGGCCTTTTGAG+TGG | - | chr1.1:34999466-34999485 | MS.gene073641:CDS | 45.0% |
| ! | TCACTGCTGTTAGCCTCTTT+GGG | - | chr1.1:35000562-35000581 | MS.gene073641:CDS | 45.0% |
| ! | TCTGGAGCAAGCTACATGTT+TGG | - | chr1.1:34999118-34999137 | MS.gene073641:CDS | 45.0% |
| !! | GGCCTTTTGAGTGGTTATCT+TGG | - | chr1.1:34999475-34999494 | MS.gene073641:CDS | 45.0% |
| !! | GGTAGTAGAGGTGTTGTCAT+AGG | - | chr1.1:34999454-34999473 | MS.gene073641:CDS | 45.0% |
| AAGACACAAGTGCCCCAAAG+AGG | + | chr1.1:35000578-35000597 | None:intergenic | 50.0% | |
| AGGGTTAAAGAGGAAGCCAG+GGG | - | chr1.1:35000488-35000507 | MS.gene073641:CDS | 50.0% | |
| ATGTGAGCATGAGAGGCCTT+GGG | + | chr1.1:35000200-35000219 | None:intergenic | 50.0% | |
| CCACAGACTCACCAATCTAG+AGG | - | chr1.1:34999880-34999899 | MS.gene073641:CDS | 50.0% | |
| CCTCTAGATTGGTGAGTCTG+TGG | + | chr1.1:34999883-34999902 | None:intergenic | 50.0% | |
| GAAGCTCAAGAGAGTGAGAG+TGG | + | chr1.1:34999188-34999207 | None:intergenic | 50.0% | |
| GGCACAATTTGTGGACTTGG+TGG | - | chr1.1:35000015-35000034 | MS.gene073641:CDS | 50.0% | |
| GGGGAGGAGTTGAATTGCAA+TGG | - | chr1.1:35000507-35000526 | MS.gene073641:CDS | 50.0% | |
| GGTGACTAAAGCTCCAGTGT+TGG | + | chr1.1:34999416-34999435 | None:intergenic | 50.0% | |
| GTGGGCACAATTTGTGGACT+TGG | - | chr1.1:35000012-35000031 | MS.gene073641:CDS | 50.0% | |
| GTTTCCTCTTCCCCAAGTAG+AGG | - | chr1.1:34999934-34999953 | MS.gene073641:CDS | 50.0% | |
| TGAGTCTGTGGCTTAGAAGG+AGG | + | chr1.1:34999871-34999890 | None:intergenic | 50.0% | |
| TGGTGAGTCTGTGGCTTAGA+AGG | + | chr1.1:34999874-34999893 | None:intergenic | 50.0% | |
| TTCACCTCTACTTGGGGAAG+AGG | + | chr1.1:34999941-34999960 | None:intergenic | 50.0% | |
| TTGCAATTCAACTCCTCCCC+TGG | + | chr1.1:35000507-35000526 | None:intergenic | 50.0% | |
| ! | CACTGCTGTTAGCCTCTTTG+GGG | - | chr1.1:35000563-35000582 | MS.gene073641:CDS | 50.0% |
| GATGTGAGCATGAGAGGCCT+TGG | + | chr1.1:35000201-35000220 | None:intergenic | 55.0% | |
| GTTAAAGAGGAAGCCAGGGG+AGG | - | chr1.1:35000491-35000510 | MS.gene073641:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 34999085 | 35000731 | 34999085 | ID=MS.gene073641 |
| chr1.1 | mRNA | 34999085 | 35000731 | 34999085 | ID=MS.gene073641.t1;Parent=MS.gene073641 |
| chr1.1 | exon | 34999085 | 35000731 | 34999085 | ID=MS.gene073641.t1.exon1;Parent=MS.gene073641.t1 |
| chr1.1 | CDS | 34999085 | 35000731 | 34999085 | ID=cds.MS.gene073641.t1;Parent=MS.gene073641.t1 |
| Gene Sequence |
| Protein sequence |
