AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene074191


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074191.t1 MTR_6g021670 100.000 191 0 0 1 191 1 191 4.74e-139 385
MS.gene074191.t1 MTR_6g085000 99.476 191 1 0 1 191 1 191 7.51e-139 385
MS.gene074191.t1 MTR_8g087480 68.182 44 6 1 145 188 51 86 1.03e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene074191.t1 AT1G48830 81.152 191 36 0 1 191 1 191 3.40e-116 328
MS.gene074191.t1 AT1G48830 81.152 191 36 0 1 191 1 191 3.40e-116 328
MS.gene074191.t1 AT3G02560 80.628 191 37 0 1 191 1 191 1.10e-113 322
MS.gene074191.t1 AT3G02560 80.628 191 37 0 1 191 1 191 1.10e-113 322
MS.gene074191.t1 AT3G02560 80.628 191 37 0 1 191 1 191 1.10e-113 322
MS.gene074191.t1 AT5G16130 81.383 188 35 0 1 188 1 188 2.94e-113 320

Find 58 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGTATTGTTTCTCTCCTT+TGG 0.234939 6.1:+5138642 None:intergenic
TACAGCCAAGTCGATGTTTC+TGG 0.252974 6.1:-5139647 MS.gene074191:intron
TGAGCAGCGGAACCTTTCTT+TGG 0.264696 6.1:+5138915 None:intergenic
CAGTATTGTTTCTCTCCTTT+GGG 0.277926 6.1:+5138643 None:intergenic
AATCCCTTCCTCAATCTATA+TGG 0.283281 6.1:+5139596 None:intergenic
AAATCCATGTCAGGCTTGTT+AGG 0.290791 6.1:-5139568 MS.gene074191:CDS
AGACATTCTCCGCAGTTTAC+AGG 0.296895 6.1:-5138608 MS.gene074191:CDS
CGCACTAGGTACCGCGTTGA+TGG 0.384231 6.1:-5138810 MS.gene074191:CDS
AAGAAAATCAGCAAGGATAA+AGG 0.404032 6.1:-5139974 MS.gene074191:CDS
CAATCCTCCTGGTGGCAATC+AGG 0.404585 6.1:+5138946 None:intergenic
GTATCCCGTTACCGAGGCTT+AGG 0.429310 6.1:-5138556 None:intergenic
GCAGTTTACAGGAAACTATC+AGG 0.445310 6.1:-5138597 MS.gene074191:CDS
TGAACCTAAGCCTCGGTAAC+GGG 0.449926 6.1:+5138552 None:intergenic
TGCTGAGCCAACTGAATTTG+AGG 0.451294 6.1:-5139951 MS.gene074191:CDS
GCAGCGGAACCTTTCTTTGG+TGG 0.457036 6.1:+5138918 None:intergenic
ATCCCTTCCTCAATCTATAT+GGG 0.481881 6.1:+5139597 None:intergenic
GTGTTACCTGCCGAGATTGT+TGG 0.493817 6.1:-5138837 MS.gene074191:CDS
AGCAGTAAGTGTACGGCTAC+GGG 0.503167 6.1:+5138887 None:intergenic
ATTGTTCGTCCACCAAAGAA+AGG 0.505744 6.1:-5138927 MS.gene074191:CDS
TTCTGGCAACCGCAAGGCTG+TGG 0.507229 6.1:-5139630 MS.gene074191:CDS
AGATTGTTGGCAAGCGCACT+AGG 0.510306 6.1:-5138824 MS.gene074191:CDS
AATCCATGTCAGGCTTGTTA+GGG 0.511974 6.1:-5139567 MS.gene074191:CDS
GATTCCGCAAAATCCATGTC+AGG 0.518236 6.1:-5139577 MS.gene074191:CDS
ACTGAATTTGAGGAATCTGT+TGG 0.520304 6.1:-5139941 MS.gene074191:CDS
ATTTGAGGAATCTGTTGGAC+AGG 0.525688 6.1:-5139936 MS.gene074191:intron
AAACAATACTGAATACAAGT+TGG 0.531067 6.1:-5138631 MS.gene074191:CDS
CAGCAGTAAGTGTACGGCTA+CGG 0.532367 6.1:+5138886 None:intergenic
CGCTTGCCAACAATCTCGGC+AGG 0.533088 6.1:+5138831 None:intergenic
GTTTGAAGATGTTCACATCA+AGG 0.540861 6.1:-5139997 None:intergenic
TCCATGTCCCATATAGATTG+AGG 0.544635 6.1:-5139604 MS.gene074191:CDS
GTGAACCTAAGCCTCGGTAA+CGG 0.554094 6.1:+5138551 None:intergenic
TGGTGGACGAACAATCCTCC+TGG 0.561389 6.1:+5138935 None:intergenic
GCTCCCTAACAAGCCTGACA+TGG 0.561527 6.1:+5139564 None:intergenic
TCCTCAATCTATATGGGACA+TGG 0.562889 6.1:+5139603 None:intergenic
ACAGATTCCTCAAATTCAGT+TGG 0.566353 6.1:+5139944 None:intergenic
ACCTAAGTGAACCTAAGCCT+CGG 0.587539 6.1:+5138545 None:intergenic
ATGTTATCCTGATTGCCACC+AGG 0.588268 6.1:-5138953 MS.gene074191:CDS
AGTGCGCTTGCCAACAATCT+CGG 0.589160 6.1:+5138827 None:intergenic
CGTGGACAGCAGTAAGTGTA+CGG 0.599592 6.1:+5138880 None:intergenic
CGAGAAGAAATTTAGCGGCA+AGG 0.600668 6.1:-5139540 MS.gene074191:intron
TGTCCCATATAGATTGAGGA+AGG 0.607696 6.1:-5139600 MS.gene074191:CDS
CGATGTTTCTGGCAACCGCA+AGG 0.612148 6.1:-5139636 MS.gene074191:CDS
GAGCTCGAGAAGAAATTTAG+CGG 0.626349 6.1:-5139545 MS.gene074191:CDS
GGTTGCCAGAAACATCGACT+TGG 0.639023 6.1:+5139642 None:intergenic
TGATGGATCAAAGATCATGA+AGG 0.639271 6.1:-5138793 MS.gene074191:intron
TGTCCACGAGGCAATGCTCG+AGG 0.642990 6.1:-5138865 MS.gene074191:CDS
GTCCCATATAGATTGAGGAA+GGG 0.647191 6.1:-5139599 MS.gene074191:CDS
ATCAAGGAAGAAAATCAGCA+AGG 0.649740 6.1:-5139981 MS.gene074191:CDS
CAGTAAGTGTACGGCTACGG+GGG 0.652122 6.1:+5138889 None:intergenic
CATCCTCGAGCATTGCCTCG+TGG 0.673479 6.1:+5138862 None:intergenic
TTATCCTGATTGCCACCAGG+AGG 0.673801 6.1:-5138950 MS.gene074191:CDS
TACACTTACTGCTGTCCACG+AGG 0.675978 6.1:-5138877 MS.gene074191:CDS
GTTTGAGTATCCCGTTACCG+AGG 0.676627 6.1:-5138562 MS.gene074191:CDS
GCAGTAAGTGTACGGCTACG+GGG 0.682631 6.1:+5138888 None:intergenic
CATGGATAACCACAGCCTTG+CGG 0.691240 6.1:+5139621 None:intergenic
TGATAGTTTCCTGTAAACTG+CGG 0.711983 6.1:+5138599 None:intergenic
TGGACGAACAATCCTCCTGG+TGG 0.731132 6.1:+5138938 None:intergenic
TGATCTTTGATCCATCAACG+CGG 0.833754 6.1:+5138799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTGAAATCAAAACATAT+TGG + chr6.1:5139406-5139425 None:intergenic 20.0%
!!! TGATTTTAATTGAATTCTTG+TGG - chr6.1:5139854-5139873 MS.gene074191:intron 20.0%
! AAACAATACTGAATACAAGT+TGG - chr6.1:5139915-5139934 MS.gene074191:intron 25.0%
! AATTAAGGATCAAAAGACAA+TGG + chr6.1:5139123-5139142 None:intergenic 25.0%
! ATGAAACATCATGCATATAA+TGG + chr6.1:5139539-5139558 None:intergenic 25.0%
! ATTGGAATTTCATTATGAAC+TGG - chr6.1:5139225-5139244 MS.gene074191:intron 25.0%
! GTTAAACAAGGTTTCAATTA+AGG + chr6.1:5139138-5139157 None:intergenic 25.0%
!!! AACTGGAATTTTGTTATGAT+TGG - chr6.1:5139207-5139226 MS.gene074191:intron 25.0%
!!! CAGTTTTAAATCAAACTGTA+GGG - chr6.1:5139492-5139511 MS.gene074191:intron 25.0%
AAGAAAATCAGCAAGGATAA+AGG - chr6.1:5138572-5138591 MS.gene074191:CDS 30.0%
AATGACTCTATACAGAACAA+AGG + chr6.1:5139050-5139069 None:intergenic 30.0%
AATGTGATTGAGGGAAATTA+CGG + chr6.1:5139172-5139191 None:intergenic 30.0%
ACATCATGCATATAATGGTA+TGG + chr6.1:5139534-5139553 None:intergenic 30.0%
GTGCTATAGTAAGTTAAACA+AGG + chr6.1:5139150-5139169 None:intergenic 30.0%
TTGTTCTGTATAGAGTCATT+AGG - chr6.1:5139050-5139069 MS.gene074191:intron 30.0%
TTTGTTTGATTGAACACAAC+AGG + chr6.1:5139813-5139832 None:intergenic 30.0%
! TTGATTGATACAGGTGTTTT+TGG - chr6.1:5139879-5139898 MS.gene074191:intron 30.0%
!!! ATTTTTGTTGTTGTTGTTGC+AGG - chr6.1:5138716-5138735 MS.gene074191:intron 30.0%
!!! GCAGTTTTAAATCAAACTGT+AGG - chr6.1:5139491-5139510 MS.gene074191:intron 30.0%
AATCCCTTCCTCAATCTATA+TGG + chr6.1:5138953-5138972 None:intergenic 35.0%
ACAGATTCCTCAAATTCAGT+TGG + chr6.1:5138605-5138624 None:intergenic 35.0%
ACTGAATTTGAGGAATCTGT+TGG - chr6.1:5138605-5138624 MS.gene074191:CDS 35.0%
ATCAAGGAAGAAAATCAGCA+AGG - chr6.1:5138565-5138584 MS.gene074191:CDS 35.0%
ATCCCTTCCTCAATCTATAT+GGG + chr6.1:5138952-5138971 None:intergenic 35.0%
ATTCCAGTTACAGTTACCAT+CGG + chr6.1:5139196-5139215 None:intergenic 35.0%
GATAACATCCTGCAAGTTAA+AGG + chr6.1:5139581-5139600 None:intergenic 35.0%
TCAGCGAGAATTACTATCAA+TGG + chr6.1:5139433-5139452 None:intergenic 35.0%
TGATAGTTTCCTGTAAACTG+CGG + chr6.1:5139950-5139969 None:intergenic 35.0%
! AACACAACAGGAGTAACTTT+TGG + chr6.1:5139801-5139820 None:intergenic 35.0%
! CAGTATTGTTTCTCTCCTTT+GGG + chr6.1:5139906-5139925 None:intergenic 35.0%
! TCAGTATTGTTTCTCTCCTT+TGG + chr6.1:5139907-5139926 None:intergenic 35.0%
! TGATGGATCAAAGATCATGA+AGG - chr6.1:5139753-5139772 MS.gene074191:intron 35.0%
! TTGATTTTCTCCTCAGTATG+AGG - chr6.1:5139459-5139478 MS.gene074191:intron 35.0%
! TTTTGTCACACTACAGTTTC+TGG - chr6.1:5139074-5139093 MS.gene074191:intron 35.0%
!! CTAATGAACCTTTAACTTGC+AGG - chr6.1:5139570-5139589 MS.gene074191:CDS 35.0%
!!! CTTGTGGTTTTGATTGATAC+AGG - chr6.1:5139870-5139889 MS.gene074191:intron 35.0%
ATTGTTCGTCCACCAAAGAA+AGG - chr6.1:5139619-5139638 MS.gene074191:CDS 40.0%
ATTTGAGGAATCTGTTGGAC+AGG - chr6.1:5138610-5138629 MS.gene074191:CDS 40.0%
CTTCGAATAACCTCATACTG+AGG + chr6.1:5139472-5139491 None:intergenic 40.0%
GAGCTCGAGAAGAAATTTAG+CGG - chr6.1:5139001-5139020 MS.gene074191:intron 40.0%
GCAGTTTACAGGAAACTATC+AGG - chr6.1:5139949-5139968 MS.gene074191:CDS 40.0%
GTCCCATATAGATTGAGGAA+GGG - chr6.1:5138947-5138966 MS.gene074191:CDS 40.0%
TCCATGTCCCATATAGATTG+AGG - chr6.1:5138942-5138961 MS.gene074191:CDS 40.0%
TCCTCAATCTATATGGGACA+TGG + chr6.1:5138946-5138965 None:intergenic 40.0%
TGATCTTTGATCCATCAACG+CGG + chr6.1:5139750-5139769 None:intergenic 40.0%
TGTCCAACACAGTGCTTAAA+CGG - chr6.1:5139345-5139364 MS.gene074191:intron 40.0%
TGTCCCATATAGATTGAGGA+AGG - chr6.1:5138946-5138965 MS.gene074191:CDS 40.0%
TTCCAGTTACAGTTACCATC+GGG + chr6.1:5139195-5139214 None:intergenic 40.0%
! AAATCCATGTCAGGCTTGTT+AGG - chr6.1:5138978-5138997 MS.gene074191:intron 40.0%
! ACAGGAGTAACTTTTGGATG+TGG + chr6.1:5139795-5139814 None:intergenic 40.0%
! TAACCGTTTAAGCACTGTGT+TGG + chr6.1:5139351-5139370 None:intergenic 40.0%
!! AATCCATGTCAGGCTTGTTA+GGG - chr6.1:5138979-5138998 MS.gene074191:intron 40.0%
!! ATCGACTTGGCTGTAGATTA+TGG + chr6.1:5138894-5138913 None:intergenic 40.0%
ACCATCGGGAATGTGATTGA+GGG + chr6.1:5139181-5139200 None:intergenic 45.0%
AGACATTCTCCGCAGTTTAC+AGG - chr6.1:5139938-5139957 MS.gene074191:CDS 45.0%
ATGTTATCCTGATTGCCACC+AGG - chr6.1:5139593-5139612 MS.gene074191:CDS 45.0%
CGAGAAGAAATTTAGCGGCA+AGG - chr6.1:5139006-5139025 MS.gene074191:intron 45.0%
GATTCCGCAAAATCCATGTC+AGG - chr6.1:5138969-5138988 MS.gene074191:intron 45.0%
TACAGCCAAGTCGATGTTTC+TGG - chr6.1:5138899-5138918 MS.gene074191:CDS 45.0%
TACCATCGGGAATGTGATTG+AGG + chr6.1:5139182-5139201 None:intergenic 45.0%
TGCTGAGCCAACTGAATTTG+AGG - chr6.1:5138595-5138614 MS.gene074191:CDS 45.0%
TTCCCGATGGTAACTGTAAC+TGG - chr6.1:5139190-5139209 MS.gene074191:intron 45.0%
! CAAGCCTGACATGGATTTTG+CGG + chr6.1:5138976-5138995 None:intergenic 45.0%
!! TGAGCTCACTTTTCAGCTCA+TGG + chr6.1:5138767-5138786 None:intergenic 45.0%
!!! ACAGGTGTTTTTGGACCCAA+AGG - chr6.1:5139888-5139907 MS.gene074191:intron 45.0%
AGATTGTTGGCAAGCGCACT+AGG - chr6.1:5139722-5139741 MS.gene074191:intron 50.0%
AGCAGTAAGTGTACGGCTAC+GGG + chr6.1:5139662-5139681 None:intergenic 50.0%
AGTGCGCTTGCCAACAATCT+CGG + chr6.1:5139722-5139741 None:intergenic 50.0%
CAGCAGTAAGTGTACGGCTA+CGG + chr6.1:5139663-5139682 None:intergenic 50.0%
CATGGATAACCACAGCCTTG+CGG + chr6.1:5138928-5138947 None:intergenic 50.0%
CGTGGACAGCAGTAAGTGTA+CGG + chr6.1:5139669-5139688 None:intergenic 50.0%
GCACTGTGTTGGACATCAGA+AGG + chr6.1:5139340-5139359 None:intergenic 50.0%
GTGTTACCTGCCGAGATTGT+TGG - chr6.1:5139709-5139728 MS.gene074191:intron 50.0%
GTTTGAGTATCCCGTTACCG+AGG - chr6.1:5139984-5140003 MS.gene074191:CDS 50.0%
TACACTTACTGCTGTCCACG+AGG - chr6.1:5139669-5139688 MS.gene074191:intron 50.0%
TCCCTCAATCACATTCCCGA+TGG - chr6.1:5139177-5139196 MS.gene074191:intron 50.0%
TGAGCAGCGGAACCTTTCTT+TGG + chr6.1:5139634-5139653 None:intergenic 50.0%
TTATCCTGATTGCCACCAGG+AGG - chr6.1:5139596-5139615 MS.gene074191:CDS 50.0%
! GGTTGCCAGAAACATCGACT+TGG + chr6.1:5138907-5138926 None:intergenic 50.0%
CAATCCTCCTGGTGGCAATC+AGG + chr6.1:5139603-5139622 None:intergenic 55.0%
CAGTAAGTGTACGGCTACGG+GGG + chr6.1:5139660-5139679 None:intergenic 55.0%
GCAGCGGAACCTTTCTTTGG+TGG + chr6.1:5139631-5139650 None:intergenic 55.0%
GCAGTAAGTGTACGGCTACG+GGG + chr6.1:5139661-5139680 None:intergenic 55.0%
GCTCCCTAACAAGCCTGACA+TGG + chr6.1:5138985-5139004 None:intergenic 55.0%
TGGACGAACAATCCTCCTGG+TGG + chr6.1:5139611-5139630 None:intergenic 55.0%
! TGGTGGACGAACAATCCTCC+TGG + chr6.1:5139614-5139633 None:intergenic 55.0%
!! CGATGTTTCTGGCAACCGCA+AGG - chr6.1:5138910-5138929 MS.gene074191:CDS 55.0%
CGCTTGCCAACAATCTCGGC+AGG + chr6.1:5139718-5139737 None:intergenic 60.0%
TGTCCACGAGGCAATGCTCG+AGG - chr6.1:5139681-5139700 MS.gene074191:intron 60.0%
TTCTGGCAACCGCAAGGCTG+TGG - chr6.1:5138916-5138935 MS.gene074191:CDS 60.0%
! CATCCTCGAGCATTGCCTCG+TGG + chr6.1:5139687-5139706 None:intergenic 60.0%
! CGCACTAGGTACCGCGTTGA+TGG - chr6.1:5139736-5139755 MS.gene074191:intron 60.0%
!! GCTACGGGGGCGCTGAGCAG+CGG + chr6.1:5139647-5139666 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 5138557 5140011 5138557 ID=MS.gene074191
chr6.1 mRNA 5138557 5140011 5138557 ID=MS.gene074191.t1;Parent=MS.gene074191
chr6.1 exon 5139937 5140011 5139937 ID=MS.gene074191.t1.exon1;Parent=MS.gene074191.t1
chr6.1 CDS 5139937 5140011 5139937 ID=cds.MS.gene074191.t1;Parent=MS.gene074191.t1
chr6.1 exon 5139757 5139830 5139757 ID=MS.gene074191.t1.exon2;Parent=MS.gene074191.t1
chr6.1 CDS 5139757 5139830 5139757 ID=cds.MS.gene074191.t1;Parent=MS.gene074191.t1
chr6.1 exon 5139541 5139664 5139541 ID=MS.gene074191.t1.exon3;Parent=MS.gene074191.t1
chr6.1 CDS 5139541 5139664 5139541 ID=cds.MS.gene074191.t1;Parent=MS.gene074191.t1
chr6.1 exon 5138794 5138976 5138794 ID=MS.gene074191.t1.exon4;Parent=MS.gene074191.t1
chr6.1 CDS 5138794 5138976 5138794 ID=cds.MS.gene074191.t1;Parent=MS.gene074191.t1
chr6.1 exon 5138557 5138676 5138557 ID=MS.gene074191.t1.exon5;Parent=MS.gene074191.t1
chr6.1 CDS 5138557 5138676 5138557 ID=cds.MS.gene074191.t1;Parent=MS.gene074191.t1
Gene Sequence

>MS.gene074191

ATGTTCACATCAAGGAAGAAAATCAGCAAGGATAAAGGTGCTGAGCCAACTGAATTTGAGGAATCTGTTGGACAGGTTGTTATACATTGATTACTATTATAAACTATTGTTTTTTCTAGTTAGTTTATTTACGAATTATGATAAAATAATAACTGTTTAATTTTTGTTGTTGTTGTTGCAGGCTTTGTTTGATCTCGAAAACACTAACCATGAGCTGAAAAGTGAGCTCAAAGATTTATACATTAATTCAGCAGTGTAAGCATGCTTGCTTTTCTTGTTGTTTGTATGAAGAAGATACATGTGTTTTTTCTTTGTCTCATGTATCTACTATTTTCCATAATCTACAGCCAAGTCGATGTTTCTGGCAACCGCAAGGCTGTGGTTATCCATGTCCCATATAGATTGAGGAAGGGATTCCGCAAAATCCATGTCAGGCTTGTTAGGGAGCTCGAGAAGAAATTTAGCGGCAAGGTATACAAATTCTTATTTACCTTTGTTCTGTATAGAGTCATTAGGTTTTTGTCACACTACAGTTTCTGGTGCTCTTTTATTTGTCAACTACTCCATTGTCTTTTGATCCTTAATTGAAACCTTGTTTAACTTACTATAGCACCGTAATTTCCCTCAATCACATTCCCGATGGTAACTGTAACTGGAATTTTGTTATGATTGGAATTTCATTATGAACTGGCTGTTTTTGATTTCTGTAATTTGTTTATGCTCTTGTTAGTTAGTTTCTGAGATTGATCGAGTCTTTCATGCTGTCATGTTGTATATAAACCTTCTGATGTCCAACACAGTGCTTAAACGGTTATTATTAAGTTAAAAACTTAAACTCTGTTGTTACCAATATGTTTTGATTTCAAATCTCAACCATTGATAGTAATTCTCGCTGAGTAGATTTGATTTTCTCCTCAGTATGAGGTTATTCGAAGCAGTTTTAAATCAAACTGTAGGGTAGTTAGTTTATGATACCATACCATTATATGCATGATGTTTCATTTTGTTGTATACTAATGAACCTTTAACTTGCAGGATGTTATCCTGATTGCCACCAGGAGGATTGTTCGTCCACCAAAGAAAGGTTCCGCTGCTCAGCGCCCCCGTAGCCGTACACTTACTGCTGTCCACGAGGCAATGCTCGAGGATGTTGTGTTACCTGCCGAGATTGTTGGCAAGCGCACTAGGTACCGCGTTGATGGATCAAAGATCATGAAGGTAAACTATCGTAACAGCCACATCCAAAAGTTACTCCTGTTGTGTTCAATCAAACAAAACTTAAATAATGAATAAGAAATGATTTTAATTGAATTCTTGTGGTTTTGATTGATACAGGTGTTTTTGGACCCAAAGGAGAGAAACAATACTGAATACAAGTTGGAGACATTCTCCGCAGTTTACAGGAAACTATCAGGCAAAGATGTTGTGTTTGAGTATCCCGTTACCGAGGCTTAG

Protein sequence

>MS.gene074191.t1

MFTSRKKISKDKGAEPTEFEESVGQALFDLENTNHELKSELKDLYINSAVQVDVSGNRKAVVIHVPYRLRKGFRKIHVRLVRELEKKFSGKDVILIATRRIVRPPKKGSAAQRPRSRTLTAVHEAMLEDVVLPAEIVGKRTRYRVDGSKIMKVFLDPKERNNTEYKLETFSAVYRKLSGKDVVFEYPVTEA