Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83444.t1 | XP_003612524.2 | 93.5 | 448 | 14 | 2 | 1 | 433 | 1 | 448 | 1.90E-233 | 818.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83444.t1 | Q5E9N0 | 45.8 | 406 | 196 | 4 | 37 | 433 | 45 | 435 | 4.0e-91 | 336.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83444.t1 | G7K5D2 | 93.5 | 448 | 14 | 2 | 1 | 433 | 1 | 448 | 1.3e-233 | 818.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83444.t1 | MTR_5g025980 | 93.527 | 448 | 14 | 2 | 1 | 433 | 1 | 448 | 0.0 | 855 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83444.t1 | AT1G31660 | 57.623 | 446 | 174 | 5 | 1 | 433 | 1 | 444 | 2.77e-168 | 480 |
Find 92 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACCTGCTGCTTTCTTTA+AGG | 0.071782 | 5.4:-17059872 | MS.gene83444:CDS |
| CAATCAACTCGCCTTCTTTC+TGG | 0.148077 | 5.4:+17062377 | None:intergenic |
| ACATCCCCTCAATGCAAAAT+TGG | 0.202176 | 5.4:-17060098 | MS.gene83444:CDS |
| TCTAGCTCTCTACTTATTTC+AGG | 0.208501 | 5.4:+17055430 | None:intergenic |
| TTTATACCAAACACTGAAAA+AGG | 0.221473 | 5.4:-17059902 | MS.gene83444:CDS |
| CCTTCTTTCTGGTGGCGTTT+TGG | 0.227594 | 5.4:+17062388 | None:intergenic |
| TGGAGCTTTGGAGGATTTCT+TGG | 0.233138 | 5.4:+17062408 | None:intergenic |
| TATTGGCATTATTCAATTCT+AGG | 0.241418 | 5.4:-17060510 | MS.gene83444:CDS |
| GGAAAATTTAAAGCTTCTTC+TGG | 0.244153 | 5.4:-17055558 | MS.gene83444:CDS |
| GGAAAAGCAAAATCATTTCT+TGG | 0.279075 | 5.4:-17055537 | MS.gene83444:intron |
| TTTGGAGGATTTCTTGGATT+TGG | 0.308529 | 5.4:+17062414 | None:intergenic |
| AAACCTGCTGCTTTCTTTAA+GGG | 0.310046 | 5.4:-17059871 | MS.gene83444:CDS |
| TCCAATTCCATTGGTACATC+TGG | 0.314698 | 5.4:+17054589 | None:intergenic |
| CTAGGACAATGCCTGTTATA+TGG | 0.320912 | 5.4:-17056597 | MS.gene83444:CDS |
| GAATCCACCAGTGCCTAAAA+TGG | 0.324933 | 5.4:-17060349 | MS.gene83444:CDS |
| TCCAGATGTACCAATGGAAT+TGG | 0.374340 | 5.4:-17054590 | MS.gene83444:CDS |
| CCGTCTGGAATGAAGGGTTC+AGG | 0.376634 | 5.4:+17062448 | None:intergenic |
| GTGGCTTGAAACAAAGCATT+TGG | 0.377398 | 5.4:+17060036 | None:intergenic |
| AGTGATTGGTGCCATATAAC+AGG | 0.387926 | 5.4:+17056586 | None:intergenic |
| GGGATGTGTTTAAATGCCTT+GGG | 0.397959 | 5.4:+17060114 | None:intergenic |
| CTCAGAGAAGCAGTCATTGT+TGG | 0.399980 | 5.4:-17058755 | MS.gene83444:CDS |
| GTTGCACTATTGAAGCTTGC+TGG | 0.400448 | 5.4:-17058073 | MS.gene83444:CDS |
| CACACCGCGAAACGAACTTC+TGG | 0.402311 | 5.4:+17062270 | None:intergenic |
| TCAAGTTAACATACCTTGAA+TGG | 0.408307 | 5.4:+17058699 | None:intergenic |
| GGGGATGTGTTTAAATGCCT+TGG | 0.424695 | 5.4:+17060113 | None:intergenic |
| CTATTCCTCCACTCCATTCA+AGG | 0.426326 | 5.4:-17058712 | MS.gene83444:intron |
| TTTATATCATCTCTCACTCT+TGG | 0.427328 | 5.4:+17059949 | None:intergenic |
| TTCAGTGCCGTCTGGAATGA+AGG | 0.443676 | 5.4:+17062441 | None:intergenic |
| TCCGGAGGATGAGAGAATAT+TGG | 0.445643 | 5.4:-17060527 | MS.gene83444:CDS |
| GCTCCGAATGTTCCCATTGT+TGG | 0.446685 | 5.4:-17062241 | MS.gene83444:CDS |
| TTGGCGTATTCAGTGCCGTC+TGG | 0.449606 | 5.4:+17062433 | None:intergenic |
| CTTCTTAGCAGATATACAAT+AGG | 0.450738 | 5.4:-17060144 | MS.gene83444:CDS |
| TGGTGTCTGTAAGTGTTATC+TGG | 0.451266 | 5.4:+17060476 | None:intergenic |
| CATCCCCTCAATGCAAAATT+GGG | 0.453102 | 5.4:-17060097 | MS.gene83444:CDS |
| TTCGTTTCGCGGTGTGGAAG+AGG | 0.454075 | 5.4:-17062263 | MS.gene83444:CDS |
| CTTTCGTATTGCAGGGTTGG+AGG | 0.455905 | 5.4:-17060168 | MS.gene83444:intron |
| ATATTATTACCTGTAGCAAC+AGG | 0.456264 | 5.4:+17060433 | None:intergenic |
| ATTATTCAATTCTAGGGACT+CGG | 0.463324 | 5.4:-17060503 | MS.gene83444:CDS |
| ACCATTGTTAAAAGATTAGC+AGG | 0.469557 | 5.4:-17060457 | MS.gene83444:CDS |
| ATTGGCATTATTCAATTCTA+GGG | 0.470535 | 5.4:-17060509 | MS.gene83444:CDS |
| GCCAATATTCTCTCATCCTC+CGG | 0.471037 | 5.4:+17060526 | None:intergenic |
| CACTTCTTGCATTTGTGCAG+AGG | 0.485206 | 5.4:-17056567 | MS.gene83444:intron |
| GTTGGTGAGGATGATGTTGA+TGG | 0.489163 | 5.4:-17062223 | MS.gene83444:CDS |
| TCACCAACAATGGGAACATT+CGG | 0.492591 | 5.4:+17062238 | None:intergenic |
| ATAAATTAGTCCAATTCCAT+TGG | 0.495060 | 5.4:+17054580 | None:intergenic |
| GAAAATCAAAGCCATGTTGA+TGG | 0.495372 | 5.4:-17062172 | MS.gene83444:CDS |
| ATTCCCTTAAAGAAAGCAGC+AGG | 0.502170 | 5.4:+17059868 | None:intergenic |
| CCAAAACGCCACCAGAAAGA+AGG | 0.502367 | 5.4:-17062388 | MS.gene83444:CDS |
| CGTCTGGAATGAAGGGTTCA+GGG | 0.506596 | 5.4:+17062449 | None:intergenic |
| ACATCATCCTCACCAACAAT+GGG | 0.511719 | 5.4:+17062229 | None:intergenic |
| CAGAAGTTCGTTTCGCGGTG+TGG | 0.512343 | 5.4:-17062269 | MS.gene83444:CDS |
| AACATCATCCTCACCAACAA+TGG | 0.514016 | 5.4:+17062228 | None:intergenic |
| TTGCACTATTGAAGCTTGCT+GGG | 0.515142 | 5.4:-17058072 | MS.gene83444:CDS |
| TTAGCAGGACCTGTTGCTAC+AGG | 0.519390 | 5.4:-17060442 | MS.gene83444:intron |
| TATCAGTTCAAAGATTATCA+AGG | 0.521679 | 5.4:-17062347 | MS.gene83444:CDS |
| TGAGATTTGAAAATGATACT+AGG | 0.522794 | 5.4:-17056615 | MS.gene83444:CDS |
| ACTATTGAAGCTTGCTGGGA+TGG | 0.524744 | 5.4:-17058068 | MS.gene83444:CDS |
| TGAACTGCAGAAGGAAGATA+AGG | 0.526034 | 5.4:-17055579 | MS.gene83444:CDS |
| ACTGCATCAACCACACGATA+CGG | 0.534526 | 5.4:+17056652 | None:intergenic |
| GCACAAATGCAAGAAGTGAT+TGG | 0.534650 | 5.4:+17056572 | None:intergenic |
| GTTACCAGAAGTTCGTTTCG+CGG | 0.535066 | 5.4:-17062274 | MS.gene83444:CDS |
| ATCAATGGCGAAGAAGAGAA+AGG | 0.561958 | 5.4:-17062485 | None:intergenic |
| ATCTTACTGAACCTGAAAAG+TGG | 0.562405 | 5.4:-17060062 | MS.gene83444:CDS |
| TTATTCAATTCTAGGGACTC+GGG | 0.566076 | 5.4:-17060502 | MS.gene83444:CDS |
| TGATTATGTACCTCACATAG+TGG | 0.567184 | 5.4:+17059838 | None:intergenic |
| TCAACTCGCCTTCTTTCTGG+TGG | 0.567972 | 5.4:+17062380 | None:intergenic |
| CTCAATGCAAAATTGGGAGG+AGG | 0.572422 | 5.4:-17060091 | MS.gene83444:CDS |
| TTTCACCTCGGTTACGACTG+TGG | 0.577449 | 5.4:+17055407 | None:intergenic |
| ATGCTTTGTTTCAAGCCACT+AGG | 0.580166 | 5.4:-17060032 | MS.gene83444:CDS |
| TGAAATTCAGGATATTACTC+CGG | 0.594239 | 5.4:-17060545 | MS.gene83444:intron |
| TCAACTCAGAGAGGTTGAAG+AGG | 0.595200 | 5.4:-17062314 | MS.gene83444:CDS |
| TGGATTATTGTGGCACAACA+AGG | 0.596993 | 5.4:-17058048 | MS.gene83444:intron |
| TCAGTGCCGTCTGGAATGAA+GGG | 0.614244 | 5.4:+17062442 | None:intergenic |
| AATATTGTTTCCACTATGTG+AGG | 0.615813 | 5.4:-17059848 | MS.gene83444:intron |
| AAAATACTTGACATATACAA+GGG | 0.619470 | 5.4:-17060321 | MS.gene83444:CDS |
| ATAAAGCAAAATGCAGCCGT+TGG | 0.620040 | 5.4:+17059920 | None:intergenic |
| CAGGAACTTCACCATCAACA+TGG | 0.623265 | 5.4:+17062161 | None:intergenic |
| TTAACATACCTTGAATGGAG+TGG | 0.627262 | 5.4:+17058704 | None:intergenic |
| CGACATTCCAGATGTACCAA+TGG | 0.627346 | 5.4:-17054596 | MS.gene83444:CDS |
| TACAATAGGCAAAATCCCCA+AGG | 0.632126 | 5.4:-17060130 | MS.gene83444:CDS |
| CCCCTCAATGCAAAATTGGG+AGG | 0.632144 | 5.4:-17060094 | MS.gene83444:CDS |
| GAATGTTCCCATTGTTGGTG+AGG | 0.634440 | 5.4:-17062236 | MS.gene83444:CDS |
| GGATGTGTTTAAATGCCTTG+GGG | 0.640141 | 5.4:+17060115 | None:intergenic |
| ACATACCTTGAATGGAGTGG+AGG | 0.640405 | 5.4:+17058707 | None:intergenic |
| AATTCAGGATATTACTCCGG+AGG | 0.641238 | 5.4:-17060542 | MS.gene83444:intron |
| CTTGCTGGGATGGATTATTG+TGG | 0.644659 | 5.4:-17058058 | MS.gene83444:CDS |
| CCTGAACCCTTCATTCCAGA+CGG | 0.647833 | 5.4:-17062448 | MS.gene83444:CDS |
| TTCAATTCTAGGGACTCGGG+TGG | 0.664674 | 5.4:-17060499 | MS.gene83444:CDS |
| AAATACTTGACATATACAAG+GGG | 0.670626 | 5.4:-17060320 | MS.gene83444:intron |
| AGCTAAGCTTCAACTCAGAG+AGG | 0.674875 | 5.4:-17062323 | MS.gene83444:CDS |
| ATATGCTTTGCCGTATCGTG+TGG | 0.675148 | 5.4:-17056662 | MS.gene83444:CDS |
| CTAGACCACAGTCGTAACCG+AGG | 0.711855 | 5.4:-17055412 | MS.gene83444:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 17054585 | 17062503 | 17054585 | ID=MS.gene83444 |
| chr5.4 | mRNA | 17054585 | 17062503 | 17054585 | ID=MS.gene83444.t1;Parent=MS.gene83444 |
| chr5.4 | exon | 17062162 | 17062503 | 17062162 | ID=MS.gene83444.t1.exon1;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17062162 | 17062503 | 17062162 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17060443 | 17060557 | 17060443 | ID=MS.gene83444.t1.exon2;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17060443 | 17060557 | 17060443 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17060321 | 17060372 | 17060321 | ID=MS.gene83444.t1.exon3;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17060321 | 17060372 | 17060321 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17059849 | 17060176 | 17059849 | ID=MS.gene83444.t1.exon4;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17059849 | 17060176 | 17059849 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17058713 | 17058792 | 17058713 | ID=MS.gene83444.t1.exon5;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17058713 | 17058792 | 17058713 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17058049 | 17058096 | 17058049 | ID=MS.gene83444.t1.exon6;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17058049 | 17058096 | 17058049 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17056568 | 17056714 | 17056568 | ID=MS.gene83444.t1.exon7;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17056568 | 17056714 | 17056568 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17055538 | 17055610 | 17055538 | ID=MS.gene83444.t1.exon8;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17055538 | 17055610 | 17055538 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17055413 | 17055458 | 17055413 | ID=MS.gene83444.t1.exon9;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17055413 | 17055458 | 17055413 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| chr5.4 | exon | 17054585 | 17054655 | 17054585 | ID=MS.gene83444.t1.exon10;Parent=MS.gene83444.t1 |
| chr5.4 | CDS | 17054585 | 17054655 | 17054585 | ID=cds.MS.gene83444.t1;Parent=MS.gene83444.t1 |
| Gene Sequence |
| Protein sequence |
