Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028153.t1 | XP_003612524.2 | 91.3 | 448 | 24 | 2 | 1 | 433 | 1 | 448 | 7.60E-227 | 796.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028153.t1 | Q13895 | 44.2 | 414 | 205 | 3 | 33 | 433 | 37 | 437 | 5.7e-90 | 332.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028153.t1 | G7K5D2 | 91.3 | 448 | 24 | 2 | 1 | 433 | 1 | 448 | 5.5e-227 | 796.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028153.t1 | MTR_5g025980 | 93.304 | 448 | 15 | 2 | 1 | 433 | 1 | 448 | 0.0 | 855 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028153.t1 | AT1G31660 | 57.623 | 446 | 174 | 5 | 1 | 433 | 1 | 444 | 2.60e-168 | 480 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACCTGCTGCTTTCTTTA+AGG | 0.071782 | 5.3:-15798274 | MS.gene028153:CDS |
| CAATCAACTCGCCTTCTTTC+TGG | 0.148077 | 5.3:+15800787 | None:intergenic |
| ACATCCCCTCAATGCAAAAT+TGG | 0.202176 | 5.3:-15798500 | MS.gene028153:CDS |
| TGGAGCTTTGGAGGATTTCT+TGG | 0.207213 | 5.3:+15800818 | None:intergenic |
| TCTAGCTCTCTACTTATTTC+AGG | 0.208501 | 5.3:+15794124 | None:intergenic |
| TTTATACCAAACACTGAAAA+AGG | 0.221473 | 5.3:-15798304 | MS.gene028153:CDS |
| TATTGGCATTATTCAATTCT+AGG | 0.241418 | 5.3:-15798912 | MS.gene028153:CDS |
| GGAAAATTTAAAGCTTCTTC+TGG | 0.244153 | 5.3:-15794252 | MS.gene028153:CDS |
| GGAAAAGCAAAATCATTTCT+TGG | 0.279075 | 5.3:-15794231 | MS.gene028153:intron |
| CTGGTGGCGCTTTGGAGCTT+TGG | 0.284383 | 5.3:+15800806 | None:intergenic |
| TTTGGAGGATTTCTTGGATT+TGG | 0.308529 | 5.3:+15800824 | None:intergenic |
| AAACCTGCTGCTTTCTTTAA+GGG | 0.310046 | 5.3:-15798273 | MS.gene028153:CDS |
| TCCAATTCCATTGGTACATC+TGG | 0.314698 | 5.3:+15793283 | None:intergenic |
| CCTTCTTTCTGGTGGCGCTT+TGG | 0.315263 | 5.3:+15800798 | None:intergenic |
| CTAGGACAATGCCTGTTATA+TGG | 0.320912 | 5.3:-15795277 | MS.gene028153:CDS |
| GAATCCACCAGTGCCTAAAA+TGG | 0.324933 | 5.3:-15798751 | MS.gene028153:CDS |
| CCGTCTGGAATGAAGGGTTC+GGG | 0.368334 | 5.3:+15800858 | None:intergenic |
| TCCAGATGTACCAATGGAAT+TGG | 0.374340 | 5.3:-15793284 | MS.gene028153:CDS |
| GTGGCTTGAAACAAAGCATT+TGG | 0.377398 | 5.3:+15798438 | None:intergenic |
| AGTGATTGGTGCCATATAAC+AGG | 0.387926 | 5.3:+15795266 | None:intergenic |
| GGGATGTGTTTAAATGCCTT+GGG | 0.397959 | 5.3:+15798516 | None:intergenic |
| GCCGTCTGGAATGAAGGGTT+CGG | 0.399114 | 5.3:+15800857 | None:intergenic |
| CTCAGAGAAGCAGTCATTGT+TGG | 0.399980 | 5.3:-15797152 | MS.gene028153:CDS |
| GTTGCACTATTGAAGCTTGC+TGG | 0.400448 | 5.3:-15796487 | MS.gene028153:CDS |
| CACACCGCGAAACGAACTTC+TGG | 0.402311 | 5.3:+15800680 | None:intergenic |
| TCAAGTTAACATACCTTGAA+TGG | 0.408307 | 5.3:+15797096 | None:intergenic |
| GGGGATGTGTTTAAATGCCT+TGG | 0.424695 | 5.3:+15798515 | None:intergenic |
| CTATTCCTCCACTCCATTCA+AGG | 0.426326 | 5.3:-15797109 | MS.gene028153:intron |
| TTTATATCATCTCTCACTCT+TGG | 0.427328 | 5.3:+15798351 | None:intergenic |
| GCTCCCAATGTTCCCATTGT+TGG | 0.441269 | 5.3:-15800651 | MS.gene028153:CDS |
| GTGGCGCTTTGGAGCTTTGG+AGG | 0.442685 | 5.3:+15800809 | None:intergenic |
| TTCAGTGCCGTCTGGAATGA+AGG | 0.443676 | 5.3:+15800851 | None:intergenic |
| TCCGGAGGATGAGAGAATAT+TGG | 0.445643 | 5.3:-15798929 | MS.gene028153:CDS |
| TTGGCGTATTCAGTGCCGTC+TGG | 0.449606 | 5.3:+15800843 | None:intergenic |
| CTTCTTAGCAGATATACAAT+AGG | 0.450738 | 5.3:-15798546 | MS.gene028153:CDS |
| TGGTGTCTGTAAGTGTTATC+TGG | 0.451266 | 5.3:+15798878 | None:intergenic |
| CATCCCCTCAATGCAAAATT+GGG | 0.453102 | 5.3:-15798499 | MS.gene028153:CDS |
| TTCGTTTCGCGGTGTGGAAG+AGG | 0.454075 | 5.3:-15800673 | MS.gene028153:CDS |
| CTTTCGTATTGCAGGGTTGG+AGG | 0.455905 | 5.3:-15798570 | MS.gene028153:intron |
| ATATTATTACCTGTAGCAAC+AGG | 0.456264 | 5.3:+15798835 | None:intergenic |
| ATTATTCAATTCTAGGGACT+CGG | 0.463324 | 5.3:-15798905 | MS.gene028153:CDS |
| ACCATTGTTAAAAGATTAGC+AGG | 0.469557 | 5.3:-15798859 | MS.gene028153:CDS |
| ATTGGCATTATTCAATTCTA+GGG | 0.470535 | 5.3:-15798911 | MS.gene028153:CDS |
| GCCAATATTCTCTCATCCTC+CGG | 0.471037 | 5.3:+15798928 | None:intergenic |
| TCACCAACAATGGGAACATT+GGG | 0.474481 | 5.3:+15800648 | None:intergenic |
| CCAAAGCGCCACCAGAAAGA+AGG | 0.478486 | 5.3:-15800798 | MS.gene028153:CDS |
| CACTTCTTGCATTTGTGCAG+AGG | 0.485206 | 5.3:-15795247 | MS.gene028153:intron |
| CTCACCAACAATGGGAACAT+TGG | 0.486985 | 5.3:+15800647 | None:intergenic |
| GTTGGTGAGGATGATGTTGA+TGG | 0.489163 | 5.3:-15800633 | MS.gene028153:CDS |
| ATAAATTAGTCCAATTCCAT+TGG | 0.495060 | 5.3:+15793274 | None:intergenic |
| GAAAATCAAAGCCATGTTGA+TGG | 0.495372 | 5.3:-15800582 | MS.gene028153:CDS |
| ATTCCCTTAAAGAAAGCAGC+AGG | 0.502170 | 5.3:+15798270 | None:intergenic |
| CATCAGTTCAAAGATTATCA+AGG | 0.509270 | 5.3:-15800757 | MS.gene028153:CDS |
| ACATCATCCTCACCAACAAT+GGG | 0.511719 | 5.3:+15800639 | None:intergenic |
| CAGAAGTTCGTTTCGCGGTG+TGG | 0.512343 | 5.3:-15800679 | MS.gene028153:CDS |
| AACATCATCCTCACCAACAA+TGG | 0.514016 | 5.3:+15800638 | None:intergenic |
| TTGCACTATTGAAGCTTGCT+GGG | 0.515142 | 5.3:-15796486 | MS.gene028153:CDS |
| TTAGCAGGACCTGTTGCTAC+AGG | 0.519390 | 5.3:-15798844 | MS.gene028153:intron |
| TGAGATTTGAAAATGATACT+AGG | 0.522794 | 5.3:-15795295 | MS.gene028153:CDS |
| ACTATTGAAGCTTGCTGGGA+TGG | 0.524744 | 5.3:-15796482 | MS.gene028153:CDS |
| TGAACTGCAGAAGGAAGATA+AGG | 0.526034 | 5.3:-15794273 | MS.gene028153:CDS |
| ACTGCATCAACCACACGATA+CGG | 0.534526 | 5.3:+15795332 | None:intergenic |
| GCACAAATGCAAGAAGTGAT+TGG | 0.534650 | 5.3:+15795252 | None:intergenic |
| GTTACCAGAAGTTCGTTTCG+CGG | 0.535066 | 5.3:-15800684 | MS.gene028153:CDS |
| ATCAATGGCGAAGAAGAGAA+AGG | 0.553195 | 5.3:-15800895 | None:intergenic |
| ATCTTACTGAACCTGAAAAG+TGG | 0.562405 | 5.3:-15798464 | MS.gene028153:CDS |
| TTATTCAATTCTAGGGACTC+GGG | 0.566076 | 5.3:-15798904 | MS.gene028153:CDS |
| TGATTATGTACCTCACATAG+TGG | 0.567184 | 5.3:+15798240 | None:intergenic |
| TCAACTCGCCTTCTTTCTGG+TGG | 0.567972 | 5.3:+15800790 | None:intergenic |
| CAATGTTCCCATTGTTGGTG+AGG | 0.571746 | 5.3:-15800646 | MS.gene028153:CDS |
| CTCAATGCAAAATTGGGAGG+AGG | 0.572422 | 5.3:-15798493 | MS.gene028153:CDS |
| TTTCACCTCGGTTACGACTG+TGG | 0.577449 | 5.3:+15794101 | None:intergenic |
| ATGCTTTGTTTCAAGCCACT+AGG | 0.580166 | 5.3:-15798434 | MS.gene028153:CDS |
| TGAAATTCAGGATATTACTC+CGG | 0.594239 | 5.3:-15798947 | MS.gene028153:intron |
| TCAACTCAGAGAGGTTGAAG+AGG | 0.595200 | 5.3:-15800724 | MS.gene028153:CDS |
| TGGATTATTGTGGCACAACA+AGG | 0.596993 | 5.3:-15796462 | MS.gene028153:intron |
| CCCGAACCCTTCATTCCAGA+CGG | 0.597498 | 5.3:-15800858 | MS.gene028153:CDS |
| TCAGTGCCGTCTGGAATGAA+GGG | 0.614244 | 5.3:+15800852 | None:intergenic |
| AATATTGTTTCCACTATGTG+AGG | 0.615813 | 5.3:-15798250 | MS.gene028153:intron |
| AAAATACTTGACATATACAA+GGG | 0.619470 | 5.3:-15798723 | MS.gene028153:CDS |
| ATAAAGCAAAATGCAGCCGT+TGG | 0.620040 | 5.3:+15798322 | None:intergenic |
| CAGGAACTTCACCATCAACA+TGG | 0.623265 | 5.3:+15800571 | None:intergenic |
| TTAACATACCTTGAATGGAG+TGG | 0.627262 | 5.3:+15797101 | None:intergenic |
| CGACATTCCAGATGTACCAA+TGG | 0.627346 | 5.3:-15793290 | MS.gene028153:CDS |
| CGTCTGGAATGAAGGGTTCG+GGG | 0.631149 | 5.3:+15800859 | None:intergenic |
| TACAATAGGCAAAATCCCCA+AGG | 0.632126 | 5.3:-15798532 | MS.gene028153:CDS |
| CCCCTCAATGCAAAATTGGG+AGG | 0.632144 | 5.3:-15798496 | MS.gene028153:CDS |
| GGATGTGTTTAAATGCCTTG+GGG | 0.640141 | 5.3:+15798517 | None:intergenic |
| ACATACCTTGAATGGAGTGG+AGG | 0.640405 | 5.3:+15797104 | None:intergenic |
| AATTCAGGATATTACTCCGG+AGG | 0.641238 | 5.3:-15798944 | MS.gene028153:intron |
| CTTGCTGGGATGGATTATTG+TGG | 0.644659 | 5.3:-15796472 | MS.gene028153:CDS |
| TTCAATTCTAGGGACTCGGG+TGG | 0.664674 | 5.3:-15798901 | MS.gene028153:CDS |
| AAATACTTGACATATACAAG+GGG | 0.670626 | 5.3:-15798722 | MS.gene028153:intron |
| AGCTAAGCTTCAACTCAGAG+AGG | 0.674875 | 5.3:-15800733 | MS.gene028153:CDS |
| ATATGCTTTGCCGTATCGTG+TGG | 0.675148 | 5.3:-15795342 | MS.gene028153:CDS |
| CTAGACCACAGTCGTAACCG+AGG | 0.711855 | 5.3:-15794106 | MS.gene028153:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 15793279 | 15800913 | 15793279 | ID=MS.gene028153 |
| chr5.3 | mRNA | 15793279 | 15800913 | 15793279 | ID=MS.gene028153.t1;Parent=MS.gene028153 |
| chr5.3 | exon | 15800572 | 15800913 | 15800572 | ID=MS.gene028153.t1.exon1;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15800572 | 15800913 | 15800572 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15798845 | 15798959 | 15798845 | ID=MS.gene028153.t1.exon2;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15798845 | 15798959 | 15798845 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15798723 | 15798774 | 15798723 | ID=MS.gene028153.t1.exon3;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15798723 | 15798774 | 15798723 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15798251 | 15798578 | 15798251 | ID=MS.gene028153.t1.exon4;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15798251 | 15798578 | 15798251 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15797110 | 15797189 | 15797110 | ID=MS.gene028153.t1.exon5;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15797110 | 15797189 | 15797110 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15796463 | 15796510 | 15796463 | ID=MS.gene028153.t1.exon6;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15796463 | 15796510 | 15796463 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15795248 | 15795394 | 15795248 | ID=MS.gene028153.t1.exon7;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15795248 | 15795394 | 15795248 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15794232 | 15794304 | 15794232 | ID=MS.gene028153.t1.exon8;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15794232 | 15794304 | 15794232 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15794107 | 15794152 | 15794107 | ID=MS.gene028153.t1.exon9;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15794107 | 15794152 | 15794107 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| chr5.3 | exon | 15793279 | 15793349 | 15793279 | ID=MS.gene028153.t1.exon10;Parent=MS.gene028153.t1 |
| chr5.3 | CDS | 15793279 | 15793349 | 15793279 | ID=cds.MS.gene028153.t1;Parent=MS.gene028153.t1 |
| Gene Sequence |
| Protein sequence |
