Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72768.t1 | RHN54593.1 | 95.1 | 452 | 20 | 1 | 1 | 450 | 1 | 452 | 4.50E-241 | 843.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72768.t1 | Q13895 | 46.4 | 386 | 192 | 4 | 33 | 404 | 37 | 421 | 1.4e-89 | 331.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72768.t1 | A0A396HQ49 | 95.1 | 452 | 20 | 1 | 1 | 450 | 1 | 452 | 3.2e-241 | 843.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72768.t1 | MTR_5g025980 | 89.831 | 472 | 22 | 2 | 1 | 470 | 1 | 448 | 0.0 | 806 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72768.t1 | AT1G31660 | 55.158 | 475 | 177 | 6 | 1 | 470 | 1 | 444 | 1.51e-168 | 482 |
Find 101 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACCTGCTGCTTTCTTTA+AGG | 0.071782 | 5.1:-16536776 | MS.gene72768:CDS |
| CAATCAACTCGCCTTCTTTC+TGG | 0.148077 | 5.1:+16539235 | None:intergenic |
| TCTAGCTCTCTACTTATTTC+AGG | 0.208501 | 5.1:+16531818 | None:intergenic |
| ACATCCCCTCGATGCAAAAT+TGG | 0.210596 | 5.1:-16537002 | MS.gene72768:CDS |
| TTTATACCAAACACTGAAAA+AGG | 0.221473 | 5.1:-16536806 | MS.gene72768:CDS |
| TTCTCTACTTCGCTTTAATA+AGG | 0.224124 | 5.1:-16531717 | MS.gene72768:CDS |
| CCTTCTTTCTGGTGGCGTTT+TGG | 0.227594 | 5.1:+16539246 | None:intergenic |
| TGGAGCTTTGGAGGATTTCT+TGG | 0.233138 | 5.1:+16539266 | None:intergenic |
| GGAAAATTTAAAGCTTCTTC+TGG | 0.244153 | 5.1:-16531946 | MS.gene72768:CDS |
| GGAAAAGCAAAATCATTTCT+TGG | 0.279075 | 5.1:-16531925 | MS.gene72768:intron |
| CCGTCTGGACTGAAGGGTTC+GGG | 0.296397 | 5.1:+16539306 | None:intergenic |
| TTTGGAGGATTTCTTGGATT+TGG | 0.308529 | 5.1:+16539272 | None:intergenic |
| AAACCTGCTGCTTTCTTTAA+GGG | 0.310046 | 5.1:-16536775 | MS.gene72768:CDS |
| TCCAATTCCATTGGTACATC+TGG | 0.314698 | 5.1:+16530978 | None:intergenic |
| CTAGGACAATGCCTGTTATA+TGG | 0.320912 | 5.1:-16532973 | MS.gene72768:CDS |
| GAATCCACCAGTGCCTAAAA+TGG | 0.324933 | 5.1:-16537253 | MS.gene72768:CDS |
| TCCAGATGTACCAATGGAAT+TGG | 0.374340 | 5.1:-16530979 | MS.gene72768:CDS |
| GCCGTCTGGACTGAAGGGTT+CGG | 0.375811 | 5.1:+16539305 | None:intergenic |
| GTGGCTTGAAACAAAGCATT+TGG | 0.377398 | 5.1:+16536940 | None:intergenic |
| TCATCAACAATGGGAGCATT+AGG | 0.386734 | 5.1:+16539096 | None:intergenic |
| AGTGATTGGTGCCATATAAC+AGG | 0.387926 | 5.1:+16532962 | None:intergenic |
| CTCAGAGAAGCAGTCATTGT+TGG | 0.399980 | 5.1:-16535660 | MS.gene72768:CDS |
| GTTGCACTATTGAAGCTTGC+TGG | 0.400448 | 5.1:-16534978 | MS.gene72768:CDS |
| GGGATGTGTTTAAATGCCTT+GGG | 0.400495 | 5.1:+16537018 | None:intergenic |
| CACACCGCGAAACGAACTTC+TGG | 0.402311 | 5.1:+16539128 | None:intergenic |
| TCAAGTTAACATACCTTGAA+TGG | 0.408307 | 5.1:+16535604 | None:intergenic |
| TTCGTTTCGCGGTGTGGAAG+AGG | 0.410858 | 5.1:-16539121 | MS.gene72768:CDS |
| GGGGATGTGTTTAAATGCCT+TGG | 0.422494 | 5.1:+16537017 | None:intergenic |
| CTATTCCTCCACTCCATTCA+AGG | 0.426326 | 5.1:-16535617 | MS.gene72768:intron |
| TTGGCGTATTCAGTGCCGTC+TGG | 0.426672 | 5.1:+16539291 | None:intergenic |
| TTTATATCATCTCTCACTCT+TGG | 0.427328 | 5.1:+16536853 | None:intergenic |
| TCCGGAGGATGAGAGAATAT+TGG | 0.445643 | 5.1:-16537431 | MS.gene72768:CDS |
| CTTCTTAGCAGATATACAAT+AGG | 0.450738 | 5.1:-16537048 | MS.gene72768:CDS |
| CATCCCCTCGATGCAAAATT+GGG | 0.451715 | 5.1:-16537001 | MS.gene72768:CDS |
| CTTTCGTATTGCAGGGTTGG+AGG | 0.455905 | 5.1:-16537072 | MS.gene72768:intron |
| ATATTATTACCTGTAGCAAC+AGG | 0.456264 | 5.1:+16537337 | None:intergenic |
| TTCAGTGCCGTCTGGACTGA+AGG | 0.457235 | 5.1:+16539299 | None:intergenic |
| TATTGGCATTATTCAATTCA+AGG | 0.459331 | 5.1:-16537414 | MS.gene72768:CDS |
| CAGTCGTAACCGAGGTGAAA+AGG | 0.463805 | 5.1:-16531792 | MS.gene72768:CDS |
| ACCATTGTTAAAAGATTAGC+AGG | 0.469557 | 5.1:-16537361 | MS.gene72768:CDS |
| ATTATTCAATTCAAGGGACT+CGG | 0.470624 | 5.1:-16537407 | MS.gene72768:CDS |
| GCCAATATTCTCTCATCCTC+CGG | 0.471037 | 5.1:+16537430 | None:intergenic |
| AAGGTTAGATATGTACTGTA+TGG | 0.480798 | 5.1:-16531698 | MS.gene72768:CDS |
| TATTAAAGCGAAGTAGAGAA+AGG | 0.482519 | 5.1:+16531720 | None:intergenic |
| CACTTCTTGCATTTGTGCAG+AGG | 0.485206 | 5.1:-16532943 | MS.gene72768:intron |
| GTTGATGAGGATGATGTTGA+TGG | 0.485474 | 5.1:-16539081 | MS.gene72768:CDS |
| ATAAATTAGTCCAATTCCAT+TGG | 0.495060 | 5.1:+16530969 | None:intergenic |
| GAAAATCAAAGCCATGTTGA+TGG | 0.495372 | 5.1:-16539030 | MS.gene72768:CDS |
| TGGTGTCTGTAAGTGTTATC+CGG | 0.501779 | 5.1:+16537380 | None:intergenic |
| ATTCCCTTAAAGAAAGCAGC+AGG | 0.502170 | 5.1:+16536772 | None:intergenic |
| CCAAAACGCCACCAGAAAGA+AGG | 0.502367 | 5.1:-16539246 | MS.gene72768:CDS |
| ATTCAAGGGACTCGGGTGGC+CGG | 0.504695 | 5.1:-16537399 | MS.gene72768:CDS |
| CAGAAGTTCGTTTCGCGGTG+TGG | 0.512343 | 5.1:-16539127 | MS.gene72768:CDS |
| TTGCACTATTGAAGCTTGCT+GGG | 0.515142 | 5.1:-16534977 | MS.gene72768:CDS |
| ATTAAAGCGAAGTAGAGAAA+GGG | 0.518203 | 5.1:+16531721 | None:intergenic |
| TTAGCAGGACCTGTTGCTAC+AGG | 0.519390 | 5.1:-16537346 | MS.gene72768:intron |
| TATCAGTTCAAAGATTATCA+AGG | 0.521679 | 5.1:-16539205 | MS.gene72768:CDS |
| TGAGATTTGAAAATGATACT+AGG | 0.522794 | 5.1:-16532991 | MS.gene72768:CDS |
| ACTATTGAAGCTTGCTGGGA+TGG | 0.524744 | 5.1:-16534973 | MS.gene72768:CDS |
| TGAACTGCAGAAGGAAGATA+AGG | 0.526034 | 5.1:-16531967 | MS.gene72768:CDS |
| ATTGGCATTATTCAATTCAA+GGG | 0.526325 | 5.1:-16537413 | MS.gene72768:CDS |
| ACATCATCCTCATCAACAAT+GGG | 0.529652 | 5.1:+16539087 | None:intergenic |
| ACTGCATCAACCACACGATA+CGG | 0.534526 | 5.1:+16533028 | None:intergenic |
| GCACAAATGCAAGAAGTGAT+TGG | 0.534650 | 5.1:+16532948 | None:intergenic |
| GTTACCAGAAGTTCGTTTCG+CGG | 0.535066 | 5.1:-16539132 | MS.gene72768:CDS |
| AACATCATCCTCATCAACAA+TGG | 0.548065 | 5.1:+16539086 | None:intergenic |
| ATCAATGGCGAAGAAGAGAA+AGG | 0.553195 | 5.1:-16539343 | None:intergenic |
| ATCTTACTGAACCTGAAAAG+TGG | 0.562405 | 5.1:-16536966 | MS.gene72768:CDS |
| TGATTATGTACCTCACATAG+TGG | 0.567184 | 5.1:+16536742 | None:intergenic |
| TCAACTCGCCTTCTTTCTGG+TGG | 0.567972 | 5.1:+16539238 | None:intergenic |
| CTCGATGCAAAATTGGGAGG+AGG | 0.574718 | 5.1:-16536995 | MS.gene72768:CDS |
| TTTCACCTCGGTTACGACTG+TGG | 0.577449 | 5.1:+16531795 | None:intergenic |
| ATGCTTTGTTTCAAGCCACT+AGG | 0.580166 | 5.1:-16536936 | MS.gene72768:CDS |
| TAATGCTCCCATTGTTGATG+AGG | 0.589522 | 5.1:-16539094 | MS.gene72768:CDS |
| CCCGAACCCTTCAGTCCAGA+CGG | 0.589645 | 5.1:-16539306 | MS.gene72768:CDS |
| TGAAATTCAGGATATTACTC+CGG | 0.594239 | 5.1:-16537449 | MS.gene72768:intron |
| TCAGTGCCGTCTGGACTGAA+GGG | 0.594882 | 5.1:+16539300 | None:intergenic |
| TCAACTCAGAGAGGTTGAAG+AGG | 0.595200 | 5.1:-16539172 | MS.gene72768:CDS |
| TTATTCAATTCAAGGGACTC+GGG | 0.595757 | 5.1:-16537406 | MS.gene72768:CDS |
| TGGATTATTGTGGCACAACA+AGG | 0.596993 | 5.1:-16534953 | MS.gene72768:intron |
| CGTCTGGACTGAAGGGTTCG+GGG | 0.599382 | 5.1:+16539307 | None:intergenic |
| AAAGCGAAGTAGAGAAAGGG+AGG | 0.615095 | 5.1:+16531724 | None:intergenic |
| AATATTGTTTCCACTATGTG+AGG | 0.615813 | 5.1:-16536752 | MS.gene72768:intron |
| AAAATACTTGACATATACAA+GGG | 0.619470 | 5.1:-16537225 | MS.gene72768:CDS |
| ATAAAGCAAAATGCAGCCGT+TGG | 0.620040 | 5.1:+16536824 | None:intergenic |
| CAGGAACTTCACCATCAACA+TGG | 0.623265 | 5.1:+16539019 | None:intergenic |
| TTAACATACCTTGAATGGAG+TGG | 0.627262 | 5.1:+16535609 | None:intergenic |
| CGACATTCCAGATGTACCAA+TGG | 0.627346 | 5.1:-16530985 | MS.gene72768:CDS |
| TACAATAGGCAAAATCCCCA+AGG | 0.632126 | 5.1:-16537034 | MS.gene72768:CDS |
| GGATGTGTTTAAATGCCTTG+GGG | 0.640141 | 5.1:+16537019 | None:intergenic |
| ACATACCTTGAATGGAGTGG+AGG | 0.640405 | 5.1:+16535612 | None:intergenic |
| AATTCAGGATATTACTCCGG+AGG | 0.641238 | 5.1:-16537446 | MS.gene72768:intron |
| CTTGCTGGGATGGATTATTG+TGG | 0.644659 | 5.1:-16534963 | MS.gene72768:CDS |
| CCCCTCGATGCAAAATTGGG+AGG | 0.647839 | 5.1:-16536998 | MS.gene72768:CDS |
| TTCAATTCAAGGGACTCGGG+TGG | 0.666090 | 5.1:-16537403 | MS.gene72768:CDS |
| AAATACTTGACATATACAAG+GGG | 0.670626 | 5.1:-16537224 | MS.gene72768:intron |
| AGCTAAGCTTCAACTCAGAG+AGG | 0.674875 | 5.1:-16539181 | MS.gene72768:CDS |
| ATATGCTTTGCCGTATCGTG+TGG | 0.675148 | 5.1:-16533038 | MS.gene72768:CDS |
| AAGCGAAGTAGAGAAAGGGA+GGG | 0.698742 | 5.1:+16531725 | None:intergenic |
| TCGTAACCGAGGTGAAAAGG+AGG | 0.711585 | 5.1:-16531789 | MS.gene72768:CDS |
| CTAGACCACAGTCGTAACCG+AGG | 0.711855 | 5.1:-16531800 | MS.gene72768:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 16530974 | 16539361 | 16530974 | ID=MS.gene72768 |
| chr5.1 | mRNA | 16530974 | 16539361 | 16530974 | ID=MS.gene72768.t1;Parent=MS.gene72768 |
| chr5.1 | exon | 16539020 | 16539361 | 16539020 | ID=MS.gene72768.t1.exon1;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16539020 | 16539361 | 16539020 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16537347 | 16537461 | 16537347 | ID=MS.gene72768.t1.exon2;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16537347 | 16537461 | 16537347 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16537225 | 16537276 | 16537225 | ID=MS.gene72768.t1.exon3;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16537225 | 16537276 | 16537225 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16536753 | 16537080 | 16536753 | ID=MS.gene72768.t1.exon4;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16536753 | 16537080 | 16536753 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16535618 | 16535697 | 16535618 | ID=MS.gene72768.t1.exon5;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16535618 | 16535697 | 16535618 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16534954 | 16535001 | 16534954 | ID=MS.gene72768.t1.exon6;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16534954 | 16535001 | 16534954 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16532944 | 16533090 | 16532944 | ID=MS.gene72768.t1.exon7;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16532944 | 16533090 | 16532944 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16531926 | 16531998 | 16531926 | ID=MS.gene72768.t1.exon8;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16531926 | 16531998 | 16531926 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16531690 | 16531846 | 16531690 | ID=MS.gene72768.t1.exon9;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16531690 | 16531846 | 16531690 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| chr5.1 | exon | 16530974 | 16531044 | 16530974 | ID=MS.gene72768.t1.exon10;Parent=MS.gene72768.t1 |
| chr5.1 | CDS | 16530974 | 16531044 | 16530974 | ID=cds.MS.gene72768.t1;Parent=MS.gene72768.t1 |
| Gene Sequence |
| Protein sequence |
