AlfalfaGEDB | MS.gene074269

M. sativa cultivar XinJiangDaYe / MS.gene074269

KEGG: K02918
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00831.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074269.t1	GAU12141.1	92.7	123	9	0	1	123	1	123	8.10E-49	203

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074269.t1	Q9M5L0	89.3	122	13	0	1	122	1	122	4.5e-48	191.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074269.t1	A0A1S2Y0G0	91.1	123	11	0	1	123	1	123	2.9e-48	200.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene074269	MS.gene066455	PPI
MS.gene074269	MS.gene036455	PPI
MS.gene066455	MS.gene074269	PPI
MS.gene029109	MS.gene074269	PPI
MS.gene67378	MS.gene074269	PPI
MS.gene021047	MS.gene074269	PPI
MS.gene75171	MS.gene074269	PPI
MS.gene90833	MS.gene074269	PPI
MS.gene036455	MS.gene074269	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074269.t1	MTR_1g115545	91.667	120	10	0	4	123	2	121	1.94e-71	209
MS.gene074269.t1	MTR_7g080090	88.618	123	14	0	1	123	1	123	3.83e-70	206
MS.gene074269.t1	MTR_1g067490	88.333	120	14	0	4	123	3	122	4.68e-68	201
MS.gene074269.t1	MTR_4g062410	86.179	123	17	0	1	123	1	123	2.68e-67	199
MS.gene074269.t1	MTR_4g072410	81.513	119	22	0	5	123	3	121	1.61e-60	182
MS.gene074269.t1	MTR_1g105880	68.889	90	17	1	11	100	2	80	2.97e-32	109
MS.gene074269.t1	MTR_5g045540	88.571	35	4	0	67	101	2	36	5.04e-14	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074269.t1	AT2G39390	84.426	122	19	0	1	122	1	122	1.73e-66	197
MS.gene074269.t1	AT3G09500	83.607	122	20	0	1	122	1	122	3.91e-65	193
MS.gene074269.t1	AT5G02610	83.607	122	20	0	1	122	1	122	5.86e-65	193
MS.gene074269.t1	AT3G55170	81.967	122	22	0	1	122	1	122	7.22e-64	190
MS.gene074269.t1	AT3G55170	81.967	122	22	0	1	122	1	122	7.22e-64	190
MS.gene074269.t1	AT3G55170	81.967	122	22	0	1	122	1	122	7.22e-64	190
MS.gene074269.t1	AT5G02610	70.345	145	20	1	1	122	1	145	2.82e-59	179
MS.gene074269.t1	AT3G55170	82.828	99	17	0	1	99	1	99	6.18e-50	154
MS.gene074269.t1	AT3G55170	82.828	99	17	0	1	99	1	99	6.18e-50	154

Find 40 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACCTTACCACTTTAATTT+TGG	0.109948	6.2:-14170629	None:intergenic
TCAATGCAGCTTTCTGCTTC+TGG	0.127063	6.2:-14170681	None:intergenic
CGAATGGCCCTGGTCTTCTT+TGG	0.190566	6.2:-14170748	None:intergenic
AGGTTCAAGGTTCACGAGTT+GGG	0.321134	6.2:+14170502	MS.gene074269:CDS
TTGATAGCATATTTCATCAT+TGG	0.326699	6.2:-14170838	None:intergenic
GACCGAGAGGGAAAAGAAAA+AGG	0.348279	6.2:+14170804	MS.gene074269:CDS
GAATGGCCCTGGTCTTCTTT+GGG	0.365204	6.2:-14170747	None:intergenic
CAATGCAGCTTTCTGCTTCT+GGG	0.368452	6.2:-14170680	None:intergenic
TGATAGCATATTTCATCATT+GGG	0.381217	6.2:-14170837	None:intergenic
TTGAGCCAACAGATTAGCTT+TGG	0.411866	6.2:-14170533	None:intergenic
TTCAACAATGGCCAGGTTCA+AGG	0.450610	6.2:+14170489	None:intergenic
CAGGTTCAAGGTTCACGAGT+TGG	0.454399	6.2:+14170501	MS.gene074269:CDS
GAAGCAGAAAGCTGCATTGA+GGG	0.465646	6.2:+14170684	MS.gene074269:CDS
AATGATGAAATATGCTATCA+AGG	0.466666	6.2:+14170840	MS.gene074269:CDS
GGTAAGGTTGTCGATTGCTC+AGG	0.467815	6.2:+14170642	MS.gene074269:CDS
GCAAAGCTAGTTCAGCTTTG+AGG	0.474694	6.2:-14170564	None:intergenic
TCCGTGTTGCTAAGCTCACA+AGG	0.500647	6.2:+14170587	MS.gene074269:CDS
TCTCCAAAATTAAAGTGGTA+AGG	0.519630	6.2:+14170626	MS.gene074269:CDS
CAACTCGTGAACCTTGAACC+TGG	0.523479	6.2:-14170500	None:intergenic
CAAGCTCTCCAAAATTAAAG+TGG	0.528338	6.2:+14170621	MS.gene074269:CDS
ATCTCCGCCCAAAGAAGACC+AGG	0.531518	6.2:+14170740	MS.gene074269:CDS
GGTTCAAGGTTCACGAGTTG+GGG	0.543424	6.2:+14170503	MS.gene074269:CDS
GAGCCTCCTACGAATGGCCC+TGG	0.544213	6.2:-14170758	None:intergenic
CTTCAACGAGGACTGATGCT+TGG	0.552440	6.2:-14170782	None:intergenic
GAAATATGCTATCAAGGCAT+AGG	0.552672	6.2:+14170846	MS.gene074269:CDS
TCGTGAATTCAACAATGGCC+AGG	0.553206	6.2:+14170482	None:intergenic
TTCTTTGGGCGGAGATCAAG+TGG	0.553377	6.2:-14170733	None:intergenic
TGGCCCTGGTCTTCTTTGGG+CGG	0.571866	6.2:-14170744	None:intergenic
CTTGGTGAGCCTCCTACGAA+TGG	0.577511	6.2:-14170764	None:intergenic
CCCTTGTGAGCTTAGCAACA+CGG	0.592776	6.2:-14170588	None:intergenic
GAAGACCAAAGCTAATCTGT+TGG	0.595836	6.2:+14170528	MS.gene074269:CDS
AGACCAGGGCCATTCGTAGG+AGG	0.605809	6.2:+14170755	MS.gene074269:CDS
TTCCCTCTCGGTCTTCAACG+AGG	0.624458	6.2:-14170794	None:intergenic
AGAAGACCAGGGCCATTCGT+AGG	0.625783	6.2:+14170752	MS.gene074269:CDS
AGAAGCAGAAAGCTGCATTG+AGG	0.626163	6.2:+14170683	MS.gene074269:CDS
CCGTGTTGCTAAGCTCACAA+GGG	0.628976	6.2:+14170588	MS.gene074269:CDS
CGTGTTGCTAAGCTCACAAG+GGG	0.656634	6.2:+14170589	MS.gene074269:CDS
GTCCTCGTTGAAGACCGAGA+GGG	0.699048	6.2:+14170792	MS.gene074269:CDS
TCTCCGCCCAAAGAAGACCA+GGG	0.718890	6.2:+14170741	MS.gene074269:CDS
AGTCCTCGTTGAAGACCGAG+AGG	0.723068	6.2:+14170791	MS.gene074269:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGATGAAATATGCTATCA+AGG	+	chr6.2:14170840-14170859	MS.gene074269:CDS	25.0%
!	TGATAGCATATTTCATCATT+GGG	-	chr6.2:14170840-14170859	None:intergenic	25.0%
!!	TTGATAGCATATTTCATCAT+TGG	-	chr6.2:14170841-14170860	None:intergenic	25.0%
	TCTCCAAAATTAAAGTGGTA+AGG	+	chr6.2:14170626-14170645	MS.gene074269:CDS	30.0%
!!	CAACCTTACCACTTTAATTT+TGG	-	chr6.2:14170632-14170651	None:intergenic	30.0%
!!	TTAATTTTGGAGAGCTTGTT+GGG	-	chr6.2:14170619-14170638	None:intergenic	30.0%
!!	TTTAATTTTGGAGAGCTTGT+TGG	-	chr6.2:14170620-14170639	None:intergenic	30.0%
	CAAGCTCTCCAAAATTAAAG+TGG	+	chr6.2:14170621-14170640	MS.gene074269:CDS	35.0%
	GAAGACCAAAGCTAATCTGT+TGG	+	chr6.2:14170528-14170547	MS.gene074269:CDS	40.0%
!	CTCCTTTTTCTTTTCCCTCT+CGG	-	chr6.2:14170809-14170828	None:intergenic	40.0%
!!	TTGAGCCAACAGATTAGCTT+TGG	-	chr6.2:14170536-14170555	None:intergenic	40.0%
	AGAAGCAGAAAGCTGCATTG+AGG	+	chr6.2:14170683-14170702	MS.gene074269:CDS	45.0%
	AGGTTCAAGGTTCACGAGTT+GGG	+	chr6.2:14170502-14170521	MS.gene074269:CDS	45.0%
	CAATGCAGCTTTCTGCTTCT+GGG	-	chr6.2:14170683-14170702	None:intergenic	45.0%
	GAAGCAGAAAGCTGCATTGA+GGG	+	chr6.2:14170684-14170703	MS.gene074269:CDS	45.0%
	GACCGAGAGGGAAAAGAAAA+AGG	+	chr6.2:14170804-14170823	MS.gene074269:CDS	45.0%
	GCAAAGCTAGTTCAGCTTTG+AGG	-	chr6.2:14170567-14170586	None:intergenic	45.0%
	TCAATGCAGCTTTCTGCTTC+TGG	-	chr6.2:14170684-14170703	None:intergenic	45.0%
	CAACTCGTGAACCTTGAACC+TGG	-	chr6.2:14170503-14170522	None:intergenic	50.0%
	CAGGTTCAAGGTTCACGAGT+TGG	+	chr6.2:14170501-14170520	MS.gene074269:CDS	50.0%
	CCCTTGTGAGCTTAGCAACA+CGG	-	chr6.2:14170591-14170610	None:intergenic	50.0%
	CTTCAACGAGGACTGATGCT+TGG	-	chr6.2:14170785-14170804	None:intergenic	50.0%
	GGTTCAAGGTTCACGAGTTG+GGG	+	chr6.2:14170503-14170522	MS.gene074269:CDS	50.0%
	TTCTTTGGGCGGAGATCAAG+TGG	-	chr6.2:14170736-14170755	None:intergenic	50.0%
!	CCGTGTTGCTAAGCTCACAA+GGG	+	chr6.2:14170588-14170607	MS.gene074269:CDS	50.0%
!	CGTGTTGCTAAGCTCACAAG+GGG	+	chr6.2:14170589-14170608	MS.gene074269:CDS	50.0%
!	GAATGGCCCTGGTCTTCTTT+GGG	-	chr6.2:14170750-14170769	None:intergenic	50.0%
!	GGTAAGGTTGTCGATTGCTC+AGG	+	chr6.2:14170642-14170661	MS.gene074269:CDS	50.0%
!	TCCGTGTTGCTAAGCTCACA+AGG	+	chr6.2:14170587-14170606	MS.gene074269:CDS	50.0%
	AGAAGACCAGGGCCATTCGT+AGG	+	chr6.2:14170752-14170771	MS.gene074269:CDS	55.0%
	AGTCCTCGTTGAAGACCGAG+AGG	+	chr6.2:14170791-14170810	MS.gene074269:CDS	55.0%
	ATCTCCGCCCAAAGAAGACC+AGG	+	chr6.2:14170740-14170759	MS.gene074269:CDS	55.0%
	CTTGGTGAGCCTCCTACGAA+TGG	-	chr6.2:14170767-14170786	None:intergenic	55.0%
	GTCCTCGTTGAAGACCGAGA+GGG	+	chr6.2:14170792-14170811	MS.gene074269:CDS	55.0%
	TCTCCGCCCAAAGAAGACCA+GGG	+	chr6.2:14170741-14170760	MS.gene074269:CDS	55.0%
	TTCCCTCTCGGTCTTCAACG+AGG	-	chr6.2:14170797-14170816	None:intergenic	55.0%
!	CGAATGGCCCTGGTCTTCTT+TGG	-	chr6.2:14170751-14170770	None:intergenic	55.0%
	AGACCAGGGCCATTCGTAGG+AGG	+	chr6.2:14170755-14170774	MS.gene074269:CDS	60.0%
!	TGGCCCTGGTCTTCTTTGGG+CGG	-	chr6.2:14170747-14170766	None:intergenic	60.0%
	GAGCCTCCTACGAATGGCCC+TGG	-	chr6.2:14170761-14170780	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	14170496	14170867	14170496	ID=MS.gene074269
chr6.2	mRNA	14170496	14170867	14170496	ID=MS.gene074269.t1;Parent=MS.gene074269
chr6.2	exon	14170496	14170867	14170496	ID=MS.gene074269.t1.exon1;Parent=MS.gene074269.t1
chr6.2	CDS	14170496	14170867	14170496	ID=cds.MS.gene074269.t1;Parent=MS.gene074269.t1

Gene Sequence	>MS.gene074269 ATGGCCAGGTTCAAGGTTCACGAGTTGGGGCAGAAGACCAAAGCTAATCTGTTGGCTCAACTCAAATACCTCAAAGCTGAACTAGCTTTGCTCCGTGTTGCTAAGCTCACAAGGGGTGCACCCAACAAGCTCTCCAAAATTAAAGTGGTAAGGTTGTCGATTGCTCAGGTTTTGACAGTGATTTCCCAGAAGCAGAAAGCTGCATTGAGGGAAGTTTACAAGAACAAGAAGTATTTGCCACTTGATCTCCGCCCAAAGAAGACCAGGGCCATTCGTAGGAGGCTCACCAAGCATCAGTCCTCGTTGAAGACCGAGAGGGAAAAGAAAAAGGAGACATATTTCCCAATGATGAAATATGCTATCAAGGCATAG
Protein sequence	>MS.gene074269.t1 MARFKVHELGQKTKANLLAQLKYLKAELALLRVAKLTRGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQSSLKTEREKKKETYFPMMKYAIKA