AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066455


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066455.t1 MTR_1g115545 98.347 121 2 0 1 121 1 121 8.33e-81 233
MS.gene066455.t1 MTR_1g067490 92.500 120 9 0 2 121 3 122 1.59e-74 217
MS.gene066455.t1 MTR_4g062410 91.667 120 10 0 2 121 4 123 2.08e-72 211
MS.gene066455.t1 MTR_7g080090 90.000 120 12 0 2 121 4 123 4.69e-71 208
MS.gene066455.t1 MTR_4g072410 85.124 121 18 0 1 121 1 121 2.70e-67 199
MS.gene066455.t1 MTR_1g105880 72.222 90 14 1 9 98 2 80 1.31e-35 117
MS.gene066455.t1 MTR_5g045540 88.571 35 4 0 65 99 2 36 5.06e-14 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066455.t1 AT2G39390 88.235 119 14 0 2 120 4 122 4.36e-70 206
MS.gene066455.t1 AT3G09500 87.395 119 15 0 2 120 4 122 1.19e-68 202
MS.gene066455.t1 AT5G02610 87.395 119 15 0 2 120 4 122 1.82e-68 202
MS.gene066455.t1 AT3G55170 85.714 119 17 0 2 120 4 122 4.19e-67 198
MS.gene066455.t1 AT3G55170 85.714 119 17 0 2 120 4 122 4.19e-67 198
MS.gene066455.t1 AT3G55170 85.714 119 17 0 2 120 4 122 4.19e-67 198
MS.gene066455.t1 AT5G02610 73.239 142 15 1 2 120 4 145 9.02e-63 188
MS.gene066455.t1 AT3G55170 86.458 96 13 0 2 97 4 99 4.75e-52 159
MS.gene066455.t1 AT3G55170 86.458 96 13 0 2 97 4 99 4.75e-52 159

Find 32 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAATGGCCCTGGTCTTCTT+TGG 0.171363 1.2:+85560281 None:intergenic
TCAAAGCAGCCTTCTGCTTC+TGG 0.210134 1.2:+85560348 None:intergenic
TTGATAGCATATTTCCTCAT+TGG 0.349490 1.2:+85559408 None:intergenic
GAATGGCCCTGGTCTTCTTT+GGG 0.358511 1.2:+85560282 None:intergenic
GACCGAGAGGGAGAAGAAAA+AGG 0.368054 1.2:-85559442 MS.gene066455:CDS
TGATAGCATATTTCCTCATT+GGG 0.379576 1.2:+85559409 None:intergenic
GAAGCAGAAGGCTGCTTTGA+GGG 0.418273 1.2:-85560345 MS.gene066455:CDS
CAAAGCAGCCTTCTGCTTCT+GGG 0.419055 1.2:+85560349 None:intergenic
GAGCCTTCTGCGAATGGCCC+TGG 0.419374 1.2:+85560271 None:intergenic
CTCCGTGTTGCTAAGGTCAC+TGG 0.447113 1.2:-85560746 MS.gene066455:CDS
GTAAAGCAAGTTCAGCTTTG+AGG 0.452854 1.2:+85560768 None:intergenic
CTTGGTGAGCCTTCTGCGAA+TGG 0.472202 1.2:+85560265 None:intergenic
AATGAGGAAATATGCTATCA+AGG 0.472756 1.2:-85559406 MS.gene066455:CDS
TGCTTTACTCCGTGTTGCTA+AGG 0.473685 1.2:-85560753 MS.gene066455:CDS
GAAATATGCTATCAAGGCAT+AGG 0.504384 1.2:-85559400 None:intergenic
AGGGTTTAATACCTGGTGCT+TGG 0.515451 1.2:+85560247 None:intergenic
CAGTGAAGGTTCACGAGTTG+AGG 0.518240 1.2:-85560829 MS.gene066455:CDS
ATCTCCGCCCAAAGAAGACC+AGG 0.531518 1.2:-85560289 MS.gene066455:CDS
CAGAAGGCTCACCAAGCACC+AGG 0.535404 1.2:-85560258 MS.gene066455:intron
TTCTTTGGGCGGAGATCAAG+TGG 0.553377 1.2:+85560296 None:intergenic
GGTGAGGTTGTCGATTGCTC+AGG 0.570678 1.2:-85560387 MS.gene066455:CDS
TGGCCCTGGTCTTCTTTGGG+CGG 0.571866 1.2:+85560285 None:intergenic
AAATATGCTATCAAGGCATA+GGG 0.572355 1.2:-85559399 None:intergenic
AGAAGCAGAAGGCTGCTTTG+AGG 0.576045 1.2:-85560346 MS.gene066455:CDS
AGACCAGGGCCATTCGCAGA+AGG 0.591558 1.2:-85560274 MS.gene066455:CDS
AGGAGACATATTTCCCAATG+AGG 0.613250 1.2:-85559422 MS.gene066455:CDS
AGTGATTTCCCAGAAGCAGA+AGG 0.617415 1.2:-85560357 MS.gene066455:CDS
CACCAGTGACCTTAGCAACA+CGG 0.624056 1.2:+85560744 None:intergenic
GTCTTCGCTGAAGACCGAGA+GGG 0.684722 1.2:-85559454 MS.gene066455:CDS
CGTGTTGCTAAGGTCACTGG+TGG 0.692805 1.2:-85560743 MS.gene066455:CDS
TCTCCGCCCAAAGAAGACCA+GGG 0.718890 1.2:-85560288 MS.gene066455:CDS
AGTCTTCGCTGAAGACCGAG+AGG 0.727322 1.2:-85559455 MS.gene066455:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTGATTATTTTATTT+GGG - chr1.2:85559906-85559925 MS.gene066455:intron 10.0%
!!! TTGAATTTTTATTTACATTT+TGG + chr1.2:85559630-85559649 None:intergenic 10.0%
!!! TTGTTTTTGATTATTTTATT+TGG - chr1.2:85559905-85559924 MS.gene066455:intron 10.0%
!! AAATTTCAGTTTGTATATAT+AGG + chr1.2:85560778-85560797 None:intergenic 15.0%
!! AACATGAATTATACATTAAA+AGG + chr1.2:85560106-85560125 None:intergenic 15.0%
!! ACATGAATTATACATTAAAA+GGG + chr1.2:85560105-85560124 None:intergenic 15.0%
!!! AGTTTTTTTTCTAAAATCAA+AGG - chr1.2:85560449-85560468 MS.gene066455:intron 15.0%
!!! TTGTTTGATTTCATAAATTA+GGG + chr1.2:85559426-85559445 None:intergenic 15.0%
!!! TTTGTTTGATTTCATAAATT+AGG + chr1.2:85559427-85559446 None:intergenic 15.0%
!! GAATGAAATCTTAAATAATG+AGG - chr1.2:85560350-85560369 MS.gene066455:CDS 20.0%
!! TAGTGAATTTCATGTAAAAA+TGG + chr1.2:85560565-85560584 None:intergenic 20.0%
!! TTTGTTTAGAAGATAATAAC+TGG - chr1.2:85560613-85560632 MS.gene066455:intron 20.0%
!!! ACATTCAATTTTATGCTTTA+AGG + chr1.2:85560536-85560555 None:intergenic 20.0%
!!! CTTTATTCTTTTATGTCAAT+TGG - chr1.2:85559651-85559670 MS.gene066455:intron 20.0%
! AACAATTTATCCTTCAGAAT+TGG + chr1.2:85559787-85559806 None:intergenic 25.0%
! CTTTCAAGGATAACATTTAA+TGG + chr1.2:85560493-85560512 None:intergenic 25.0%
! GATTGTGTGATGATTATAAT+GGG - chr1.2:85559827-85559846 MS.gene066455:intron 25.0%
! TGATTATTACTGTTCTTTCA+AGG + chr1.2:85560507-85560526 None:intergenic 25.0%
! TGATTGTGTGATGATTATAA+TGG - chr1.2:85559826-85559845 MS.gene066455:intron 25.0%
! TTAGAAGATAATAACTGGAT+TGG - chr1.2:85560618-85560637 MS.gene066455:intron 25.0%
!! TTTGATTTCATAAATTAGGG+TGG + chr1.2:85559423-85559442 None:intergenic 25.0%
AAAACAACCAATGGTGTAAA+AGG + chr1.2:85560667-85560686 None:intergenic 30.0%
AATGAGGAAATATGCTATCA+AGG - chr1.2:85560924-85560943 MS.gene066455:intron 30.0%
ACATACAAGCAAAGAGAAAA+AGG + chr1.2:85560746-85560765 None:intergenic 30.0%
AGGATAACATTTAATGGTGT+AGG + chr1.2:85560487-85560506 None:intergenic 30.0%
CATTAAAAGGGTTTAATACC+TGG + chr1.2:85560093-85560112 None:intergenic 30.0%
GAAGGATAAATTGTTCCTTT+CGG - chr1.2:85559792-85559811 MS.gene066455:intron 30.0%
TATCCTTCAGAATTGGTAAA+TGG + chr1.2:85559780-85559799 None:intergenic 30.0%
TCATAAATTAGGGTGGTTTA+GGG + chr1.2:85559416-85559435 None:intergenic 30.0%
TGATAGCATATTTCCTCATT+GGG + chr1.2:85560924-85560943 None:intergenic 30.0%
TTCATAAATTAGGGTGGTTT+AGG + chr1.2:85559417-85559436 None:intergenic 30.0%
TTTCGTCTCAAGTACTATTT+AGG - chr1.2:85560318-85560337 MS.gene066455:CDS 30.0%
! ATTATAGCCTTTTACACCAT+TGG - chr1.2:85560657-85560676 MS.gene066455:intron 30.0%
! GATCTGTATCATTTGGAATA+AGG + chr1.2:85560387-85560406 None:intergenic 30.0%
! TTGATAGCATATTTCCTCAT+TGG + chr1.2:85560925-85560944 None:intergenic 30.0%
!! GCACGATTGTTCTAAAAATA+AGG + chr1.2:85559707-85559726 None:intergenic 30.0%
!! TAACTGGATTGGTTTATTGT+TGG - chr1.2:85560629-85560648 MS.gene066455:intron 30.0%
!! TTTATTTGGGTTTTGCAGTA+AGG - chr1.2:85559919-85559938 MS.gene066455:intron 30.0%
!! TTTTCAACCTGATTTGTCTT+GGG - chr1.2:85560683-85560702 MS.gene066455:intron 30.0%
!!! GTTTTTGTTTTGTGACTGTA+AGG - chr1.2:85559852-85559871 MS.gene066455:intron 30.0%
AGCAAAAACCACAGATACAT+TGG + chr1.2:85560159-85560178 None:intergenic 35.0%
ATACACCAATCACCAATTGA+TGG + chr1.2:85560251-85560270 None:intergenic 35.0%
GAAAAACAAAGCTGATCTGT+TGG - chr1.2:85559526-85559545 MS.gene066455:intron 35.0%
GCAAAAACCACAGATACATT+GGG + chr1.2:85560158-85560177 None:intergenic 35.0%
GCAAAGAGAAAAAGGTTACA+AGG + chr1.2:85560738-85560757 None:intergenic 35.0%
TATTGTGACCATCCATCAAT+TGG - chr1.2:85560236-85560255 MS.gene066455:intron 35.0%
TGAATATCCCAAGACAAATC+AGG + chr1.2:85560693-85560712 None:intergenic 35.0%
TTCAAGCAGAACAGTATGTT+TGG - chr1.2:85560818-85560837 MS.gene066455:CDS 35.0%
! AATTGTTCCTTTCGGTGTAA+TGG - chr1.2:85559800-85559819 MS.gene066455:intron 35.0%
!! AATTGGTGCTATGATCGTAA+AGG - chr1.2:85559668-85559687 MS.gene066455:intron 35.0%
!! ATCAGGTTGAAAACAACCAA+TGG + chr1.2:85560676-85560695 None:intergenic 35.0%
!! GTTTTCAACCTGATTTGTCT+TGG - chr1.2:85560682-85560701 MS.gene066455:intron 35.0%
!! TACATTTTGGAGAGCTTGTT+GGG + chr1.2:85559617-85559636 None:intergenic 35.0%
!! TTACATTTTGGAGAGCTTGT+TGG + chr1.2:85559618-85559637 None:intergenic 35.0%
!!! TGTGTTTTGATTTGCAGTGA+AGG - chr1.2:85559487-85559506 MS.gene066455:intron 35.0%
!!! TTTGTTTTGTGACTGTAAGG+TGG - chr1.2:85559855-85559874 MS.gene066455:intron 35.0%
AAAACCACAGATACATTGGG+AGG + chr1.2:85560155-85560174 None:intergenic 40.0%
ACCAATCACCAATTGATGGA+TGG + chr1.2:85560247-85560266 None:intergenic 40.0%
AGGAGACATATTTCCCAATG+AGG - chr1.2:85560908-85560927 MS.gene066455:intron 40.0%
ATCAAAGCCATTACACCGAA+AGG + chr1.2:85559810-85559829 None:intergenic 40.0%
ATGAAATTCACTACACGACC+AGG - chr1.2:85560572-85560591 MS.gene066455:intron 40.0%
CAGAAAATTGTACAGCAACC+TGG + chr1.2:85560593-85560612 None:intergenic 40.0%
CGTCCATTTACCAATTCTGA+AGG - chr1.2:85559774-85559793 MS.gene066455:intron 40.0%
GTAAAGCAAGTTCAGCTTTG+AGG + chr1.2:85559565-85559584 None:intergenic 40.0%
! ACCATCCATCAATTGGTGAT+TGG - chr1.2:85560243-85560262 MS.gene066455:intron 40.0%
!! ATTTGGGTTTTGCAGTAAGG+TGG - chr1.2:85559922-85559941 MS.gene066455:intron 40.0%
AGGGTTTAATACCTGGTGCT+TGG + chr1.2:85560086-85560105 None:intergenic 45.0%
AGTGATTTCCCAGAAGCAGA+AGG - chr1.2:85559973-85559992 MS.gene066455:intron 45.0%
ATACAGATCCTGCAGGTGTA+GGG - chr1.2:85560398-85560417 MS.gene066455:intron 45.0%
CCAAATGATACAGATCCTGC+AGG - chr1.2:85560391-85560410 MS.gene066455:CDS 45.0%
CCTGCAGGATCTGTATCATT+TGG + chr1.2:85560394-85560413 None:intergenic 45.0%
TACACCTCCCAATGTATCTG+TGG - chr1.2:85560148-85560167 MS.gene066455:intron 45.0%
! CTCCTTTTTCTTCTCCCTCT+CGG + chr1.2:85560893-85560912 None:intergenic 45.0%
! TGCTTTACTCCGTGTTGCTA+AGG - chr1.2:85559577-85559596 MS.gene066455:intron 45.0%
CAAAGCAGCCTTCTGCTTCT+GGG + chr1.2:85559984-85560003 None:intergenic 50.0%
CACCAGTGACCTTAGCAACA+CGG + chr1.2:85559589-85559608 None:intergenic 50.0%
CAGTGAAGGTTCACGAGTTG+AGG - chr1.2:85559501-85559520 MS.gene066455:intron 50.0%
GACCGAGAGGGAGAAGAAAA+AGG - chr1.2:85560888-85560907 MS.gene066455:intron 50.0%
GATACAGATCCTGCAGGTGT+AGG - chr1.2:85560397-85560416 MS.gene066455:intron 50.0%
TCAAAGCAGCCTTCTGCTTC+TGG + chr1.2:85559985-85560004 None:intergenic 50.0%
TTCTTTGGGCGGAGATCAAG+TGG + chr1.2:85560037-85560056 None:intergenic 50.0%
! AGAAGCAGAAGGCTGCTTTG+AGG - chr1.2:85559984-85560003 MS.gene066455:intron 50.0%
! GAAGCAGAAGGCTGCTTTGA+GGG - chr1.2:85559985-85560004 MS.gene066455:intron 50.0%
! GAATGGCCCTGGTCTTCTTT+GGG + chr1.2:85560051-85560070 None:intergenic 50.0%
! GGTTTTGCAGTAAGGTGGTG+AGG - chr1.2:85559927-85559946 MS.gene066455:intron 50.0%
AGTCTTCGCTGAAGACCGAG+AGG - chr1.2:85560875-85560894 MS.gene066455:intron 55.0%
ATCCTGCAGGTGTAGGGAGA+AGG - chr1.2:85560404-85560423 MS.gene066455:intron 55.0%
ATCCTTCTCCCTACACCTGC+AGG + chr1.2:85560409-85560428 None:intergenic 55.0%
ATCTCCGCCCAAAGAAGACC+AGG - chr1.2:85560041-85560060 MS.gene066455:intron 55.0%
CTTGGTGAGCCTTCTGCGAA+TGG + chr1.2:85560068-85560087 None:intergenic 55.0%
GTCTTCGCTGAAGACCGAGA+GGG - chr1.2:85560876-85560895 MS.gene066455:intron 55.0%
TCTCCGCCCAAAGAAGACCA+GGG - chr1.2:85560042-85560061 MS.gene066455:intron 55.0%
! CGAATGGCCCTGGTCTTCTT+TGG + chr1.2:85560052-85560071 None:intergenic 55.0%
! CGTGTTGCTAAGGTCACTGG+TGG - chr1.2:85559587-85559606 MS.gene066455:intron 55.0%
! CTCCGTGTTGCTAAGGTCAC+TGG - chr1.2:85559584-85559603 MS.gene066455:intron 55.0%
! GGTGAGGTTGTCGATTGCTC+AGG - chr1.2:85559943-85559962 MS.gene066455:intron 55.0%
AGACCAGGGCCATTCGCAGA+AGG - chr1.2:85560056-85560075 MS.gene066455:intron 60.0%
CAGAAGGCTCACCAAGCACC+AGG - chr1.2:85560072-85560091 MS.gene066455:intron 60.0%
! TGGCCCTGGTCTTCTTTGGG+CGG + chr1.2:85560048-85560067 None:intergenic 60.0%
GAGCCTTCTGCGAATGGCCC+TGG + chr1.2:85560062-85560081 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 85559401 85560951 85559401 ID=MS.gene066455
chr1.2 mRNA 85559401 85560951 85559401 ID=MS.gene066455.t1;Parent=MS.gene066455
chr1.2 exon 85560948 85560951 85560948 ID=MS.gene066455.t1.exon1;Parent=MS.gene066455.t1
chr1.2 CDS 85560948 85560951 85560948 ID=cds.MS.gene066455.t1;Parent=MS.gene066455.t1
chr1.2 exon 85560719 85560848 85560719 ID=MS.gene066455.t1.exon2;Parent=MS.gene066455.t1
chr1.2 CDS 85560719 85560848 85560719 ID=cds.MS.gene066455.t1;Parent=MS.gene066455.t1
chr1.2 exon 85560259 85560415 85560259 ID=MS.gene066455.t1.exon3;Parent=MS.gene066455.t1
chr1.2 CDS 85560259 85560415 85560259 ID=cds.MS.gene066455.t1;Parent=MS.gene066455.t1
chr1.2 exon 85559401 85559475 85559401 ID=MS.gene066455.t1.exon4;Parent=MS.gene066455.t1
chr1.2 CDS 85559401 85559475 85559401 ID=cds.MS.gene066455.t1;Parent=MS.gene066455.t1
Gene Sequence

>MS.gene066455

ATGGGTAAATTTCCCTAAACCACCCTAATTTATGAAATCAAACAAACAATTTCTCATAATCAATCAATCAATTTCACTAATTAGCATGTGTTTTGATTTGCAGTGAAGGTTCACGAGTTGAGGCAGAAAAACAAAGCTGATCTGTTGGCTCAGCTCAAAGACCTCAAAGCTGAACTTGCTTTACTCCGTGTTGCTAAGGTCACTGGTGGTGCACCCAACAAGCTCTCCAAAATGTAAATAAAAATTCAATCTTTATTCTTTTATGTCAATTGGTGCTATGATCGTAAAGGTGTTTTGACGTGACCTTATTTTTAGAACAATCGTGCAGTTTTGCTCAACAAAGATCACTTGTTGTTGTGTATGTAATGTATTGCGTCCATTTACCAATTCTGAAGGATAAATTGTTCCTTTCGGTGTAATGGCTTTGATTGTGTGATGATTATAATGGGTTGTTTTTGTTTTGTGACTGTAAGGTGGTGTTTGTGTTAATGATTGATTGATGTGTTGTTTTTGATTATTTTATTTGGGTTTTGCAGTAAGGTGGTGAGGTTGTCGATTGCTCAGGTGTTGACAGTGATTTCCCAGAAGCAGAAGGCTGCTTTGAGGGAAGTTTACAAGAACAAGAAGTATTTGCCACTTGATCTCCGCCCAAAGAAGACCAGGGCCATTCGCAGAAGGCTCACCAAGCACCAGGTATTAAACCCTTTTAATGTATAATTCATGTTAAAGTTCATTGTTATAAACATATACACCTCCCAATGTATCTGTGGTTTTTGCTTTTGACTGCATTGATGCATTTCATTGTTTTTTTTTTTTTTTTTTTCAATTCATATTTTATTGTGACCATCCATCAATTGGTGATTGGTGTATATTAGCTTTAATGAGATCTCTAATACGATGCTTTAGAGTAATCTGATTTTCGTCTCAAGTACTATTTAGGAGTATGAATGAATGAAATCTTAAATAATGAGGTGCTTCTTGTTCCTTATTCCAAATGATACAGATCCTGCAGGTGTAGGGAGAAGGATTATTGAATTTGACAGTGCTTAGTTTTTTTTCTAAAATCAAAGGATTTCAAGTTGACCTACACCATTAAATGTTATCCTTGAAAGAACAGTAATAATCAGTAGTACCTTAAAGCATAAAATTGAATGTCAAAATCCATTTTTACATGAAATTCACTACACGACCAGGTTGCTGTACAATTTTCTGTTTGTTTAGAAGATAATAACTGGATTGGTTTATTGTTGGATGCTATTATAGCCTTTTACACCATTGGTTGTTTTCAACCTGATTTGTCTTGGGATATTCAATCTTACATGAGTTTAGTCTTTCCTTGTAACCTTTTTCTCTTTGCTTGTATGTCAATCTCTTCCTATATATACAAACTGAAATTTTCTTGTGTAATGATTACATATTCAAGCAGAACAGTATGTTTGGATTGTTACTTATGATATGTTTTACTTGTTCATGCAGTCTTCGCTGAAGACCGAGAGGGAGAAGAAAAAGGAGACATATTTCCCAATGAGGAAATATGCTATCAAGGCATAG

Protein sequence

>MS.gene066455.t1

MVKVHELRQKNKADLLAQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQSSLKTEREKKKETYFPMRKYAIKA