AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90833


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90833.t1 MTR_1g115545 98.347 121 2 0 1 121 1 121 8.33e-81 233
MS.gene90833.t1 MTR_1g067490 92.500 120 9 0 2 121 3 122 1.59e-74 217
MS.gene90833.t1 MTR_4g062410 91.667 120 10 0 2 121 4 123 2.08e-72 211
MS.gene90833.t1 MTR_7g080090 90.000 120 12 0 2 121 4 123 4.69e-71 208
MS.gene90833.t1 MTR_4g072410 85.124 121 18 0 1 121 1 121 2.70e-67 199
MS.gene90833.t1 MTR_1g105880 72.222 90 14 1 9 98 2 80 1.31e-35 117
MS.gene90833.t1 MTR_5g045540 88.571 35 4 0 65 99 2 36 5.06e-14 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90833.t1 AT2G39390 88.235 119 14 0 2 120 4 122 4.36e-70 206
MS.gene90833.t1 AT3G09500 87.395 119 15 0 2 120 4 122 1.19e-68 202
MS.gene90833.t1 AT5G02610 87.395 119 15 0 2 120 4 122 1.82e-68 202
MS.gene90833.t1 AT3G55170 85.714 119 17 0 2 120 4 122 4.19e-67 198
MS.gene90833.t1 AT3G55170 85.714 119 17 0 2 120 4 122 4.19e-67 198
MS.gene90833.t1 AT3G55170 85.714 119 17 0 2 120 4 122 4.19e-67 198
MS.gene90833.t1 AT5G02610 73.239 142 15 1 2 120 4 145 9.02e-63 188
MS.gene90833.t1 AT3G55170 86.458 96 13 0 2 97 4 99 4.75e-52 159
MS.gene90833.t1 AT3G55170 86.458 96 13 0 2 97 4 99 4.75e-52 159

Find 32 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAATGGCCCTGGTCTTCTT+TGG 0.171363 1.4:-87759782 None:intergenic
TCAAAGCAGCCTTCTGCTTC+TGG 0.210134 1.4:-87759715 None:intergenic
TTGATAGCATATTTCCTCAT+TGG 0.349490 1.4:-87760652 None:intergenic
GAATGGCCCTGGTCTTCTTT+GGG 0.358511 1.4:-87759781 None:intergenic
GACCGAGAGGGAGAAGAAAA+AGG 0.368054 1.4:+87760618 MS.gene90833:CDS
TGATAGCATATTTCCTCATT+GGG 0.379576 1.4:-87760651 None:intergenic
GAAGCAGAAGGCTGCTTTGA+GGG 0.418273 1.4:+87759718 MS.gene90833:CDS
CAAAGCAGCCTTCTGCTTCT+GGG 0.419055 1.4:-87759714 None:intergenic
GAGCCTTCTGCGAATGGCCC+TGG 0.419374 1.4:-87759792 None:intergenic
CTCCGTGTTGCTAAGGTCAC+TGG 0.447113 1.4:+87759340 MS.gene90833:CDS
GTAAAGCAAGTTCAGCTTTG+AGG 0.452854 1.4:-87759318 None:intergenic
CTTGGTGAGCCTTCTGCGAA+TGG 0.472202 1.4:-87759798 None:intergenic
AATGAGGAAATATGCTATCA+AGG 0.472756 1.4:+87760654 MS.gene90833:CDS
TGCTTTACTCCGTGTTGCTA+AGG 0.473685 1.4:+87759333 MS.gene90833:CDS
GAAATATGCTATCAAGGCAT+AGG 0.504384 1.4:+87760660 MS.gene90833:CDS
AGGGTTTAATACCTGGTGCT+TGG 0.515451 1.4:-87759816 None:intergenic
CAGTGAAGGTTCACGAGTTG+AGG 0.518240 1.4:+87759257 MS.gene90833:intron
ATCTCCGCCCAAAGAAGACC+AGG 0.531518 1.4:+87759774 MS.gene90833:CDS
CAGAAGGCTCACCAAGCACC+AGG 0.535404 1.4:+87759805 MS.gene90833:CDS
TTCTTTGGGCGGAGATCAAG+TGG 0.553377 1.4:-87759767 None:intergenic
GGTGAGGTTGTCGATTGCTC+AGG 0.570678 1.4:+87759676 MS.gene90833:CDS
TGGCCCTGGTCTTCTTTGGG+CGG 0.571866 1.4:-87759778 None:intergenic
AAATATGCTATCAAGGCATA+GGG 0.572355 1.4:+87760661 MS.gene90833:CDS
AGAAGCAGAAGGCTGCTTTG+AGG 0.576045 1.4:+87759717 MS.gene90833:CDS
AGACCAGGGCCATTCGCAGA+AGG 0.591558 1.4:+87759789 MS.gene90833:CDS
AGGAGACATATTTCCCAATG+AGG 0.613250 1.4:+87760638 MS.gene90833:CDS
AGTGATTTCCCAGAAGCAGA+AGG 0.617415 1.4:+87759706 MS.gene90833:CDS
CACCAGTGACCTTAGCAACA+CGG 0.624056 1.4:-87759342 None:intergenic
GTCTTCGCTGAAGACCGAGA+GGG 0.684722 1.4:+87760606 MS.gene90833:intron
CGTGTTGCTAAGGTCACTGG+TGG 0.692805 1.4:+87759343 MS.gene90833:CDS
TCTCCGCCCAAAGAAGACCA+GGG 0.718890 1.4:+87759775 MS.gene90833:CDS
AGTCTTCGCTGAAGACCGAG+AGG 0.727322 1.4:+87760605 MS.gene90833:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTGATTATTTTATTT+GGG + chr1.4:87759639-87759658 MS.gene90833:intron 10.0%
!!! TTGAATTTTTATTTACATTT+TGG - chr1.4:87759386-87759405 None:intergenic 10.0%
!!! TTGTTTTTGATTATTTTATT+TGG + chr1.4:87759638-87759657 MS.gene90833:intron 10.0%
!! AAATTTCAGTTTGTATATAT+AGG - chr1.4:87760508-87760527 None:intergenic 15.0%
!! AACATGAATTATACATTAAA+AGG - chr1.4:87759839-87759858 None:intergenic 15.0%
!! ACATGAATTATACATTAAAA+GGG - chr1.4:87759838-87759857 None:intergenic 15.0%
!! GAATGAAATCTTAAATAATG+AGG + chr1.4:87760080-87760099 MS.gene90833:intron 20.0%
!! TAGTGAATTTCATGTAAAAA+TGG - chr1.4:87760295-87760314 None:intergenic 20.0%
!! TTAGAAGATAATAACTAGAT+TGG + chr1.4:87760348-87760367 MS.gene90833:intron 20.0%
!!! ACATTCAATTTTATGTGTTA+AGG - chr1.4:87760266-87760285 None:intergenic 20.0%
!!! CTTTATTCTTTTATGTCAAT+TGG + chr1.4:87759407-87759426 MS.gene90833:intron 20.0%
!!! TTGTTTGATTTCAGAAATTA+GGG - chr1.4:87759182-87759201 None:intergenic 20.0%
!!! TTTGTTTGATTTCAGAAATT+AGG - chr1.4:87759183-87759202 None:intergenic 20.0%
! AACAATTTATCCTTCAGAAT+TGG - chr1.4:87759520-87759539 None:intergenic 25.0%
! CTTTCAAGGATAACATTTAA+TGG - chr1.4:87760223-87760242 None:intergenic 25.0%
! GATTGTGTGATGATTATAAT+GGG + chr1.4:87759560-87759579 MS.gene90833:intron 25.0%
! TGATTATTACTGTTCTTTCA+AGG - chr1.4:87760237-87760256 None:intergenic 25.0%
! TGATTGTGTGATGATTATAA+TGG + chr1.4:87759559-87759578 MS.gene90833:intron 25.0%
!! TTTTGTCTCAAGTACTATTT+AGG + chr1.4:87760048-87760067 MS.gene90833:intron 25.0%
!!! AGTTTTCTTTCTAAAATCGA+AGG + chr1.4:87760179-87760198 MS.gene90833:intron 25.0%
!!! TAACTAGATTGGTTTATTGT+TGG + chr1.4:87760359-87760378 MS.gene90833:intron 25.0%
!!! TTTATTTGGGTTTTTCAGTA+AGG + chr1.4:87759652-87759671 MS.gene90833:intron 25.0%
AAAACAACCAATGGTGTAAA+AGG - chr1.4:87760397-87760416 None:intergenic 30.0%
AATGAGGAAATATGCTATCA+AGG + chr1.4:87760654-87760673 MS.gene90833:CDS 30.0%
ACATACAAGCAAAGAGAAAA+AGG - chr1.4:87760476-87760495 None:intergenic 30.0%
AGGATAACATTTAATGGTGT+AGG - chr1.4:87760217-87760236 None:intergenic 30.0%
CATTAAAAGGGTTTAATACC+TGG - chr1.4:87759826-87759845 None:intergenic 30.0%
GAAGGATAAATTGTTCCTTT+CGG + chr1.4:87759525-87759544 MS.gene90833:intron 30.0%
TATCCTTCAGAATTGGTAAA+TGG - chr1.4:87759513-87759532 None:intergenic 30.0%
TGATAGCATATTTCCTCATT+GGG - chr1.4:87760654-87760673 None:intergenic 30.0%
! ATTATAGCCTTTTACACCAT+TGG + chr1.4:87760387-87760406 MS.gene90833:intron 30.0%
! GATCTGTATCATTTGGAATA+AGG - chr1.4:87760117-87760136 None:intergenic 30.0%
! TTGATAGCATATTTCCTCAT+TGG - chr1.4:87760655-87760674 None:intergenic 30.0%
! TTTGATTTCAGAAATTAGGG+TGG - chr1.4:87759179-87759198 None:intergenic 30.0%
!! TTTTCAACCTGATTTGTCTT+GGG + chr1.4:87760413-87760432 MS.gene90833:intron 30.0%
!!! GTTTTTGTTTTGTGTCTGTA+AGG + chr1.4:87759585-87759604 MS.gene90833:intron 30.0%
AGCAAAAACCACAGATACAT+TGG - chr1.4:87759892-87759911 None:intergenic 35.0%
ATACACCAATCACCAATTGA+TGG - chr1.4:87759981-87760000 None:intergenic 35.0%
GAAAAACAAAGCTGATCTGT+TGG + chr1.4:87759282-87759301 MS.gene90833:CDS 35.0%
GCAAAAACCACAGATACATT+GGG - chr1.4:87759891-87759910 None:intergenic 35.0%
GCAAAGAGAAAAAGGTTACA+AGG - chr1.4:87760468-87760487 None:intergenic 35.0%
TATTGTGACCATCCATCAAT+TGG + chr1.4:87759966-87759985 MS.gene90833:intron 35.0%
TCAGAAATTAGGGTGGTTTA+GGG - chr1.4:87759172-87759191 None:intergenic 35.0%
TGAATATCCCAAGACAAATC+AGG - chr1.4:87760423-87760442 None:intergenic 35.0%
TTCAAGCAGAACAGTATGTT+TGG + chr1.4:87760548-87760567 MS.gene90833:intron 35.0%
TTCAGAAATTAGGGTGGTTT+AGG - chr1.4:87759173-87759192 None:intergenic 35.0%
! AATTGTTCCTTTCGGTGTAA+TGG + chr1.4:87759533-87759552 MS.gene90833:intron 35.0%
! ACTGCATTGATGCATTTCAT+TGG + chr1.4:87759916-87759935 MS.gene90833:intron 35.0%
!! AATTGGTGCTATGATCGTAA+AGG + chr1.4:87759424-87759443 MS.gene90833:intron 35.0%
!! ATCAGGTTGAAAACAACCAA+TGG - chr1.4:87760406-87760425 None:intergenic 35.0%
!! ATTTGGGTTTTTCAGTAAGG+TGG + chr1.4:87759655-87759674 MS.gene90833:intron 35.0%
!! GTTTTCAACCTGATTTGTCT+TGG + chr1.4:87760412-87760431 MS.gene90833:intron 35.0%
!! TACATTTTGGAGAGCTTGTT+GGG - chr1.4:87759373-87759392 None:intergenic 35.0%
!! TTACATTTTGGAGAGCTTGT+TGG - chr1.4:87759374-87759393 None:intergenic 35.0%
!!! TGTGTTTTGATTTGCAGTGA+AGG + chr1.4:87759243-87759262 MS.gene90833:intron 35.0%
!!! TTTGTTTTGTGTCTGTAAGG+TGG + chr1.4:87759588-87759607 MS.gene90833:intron 35.0%
AAAACCACAGATACATTGGG+AGG - chr1.4:87759888-87759907 None:intergenic 40.0%
ACCAATCACCAATTGATGGA+TGG - chr1.4:87759977-87759996 None:intergenic 40.0%
AGGAGACATATTTCCCAATG+AGG + chr1.4:87760638-87760657 MS.gene90833:CDS 40.0%
ATCAAAGCCATTACACCGAA+AGG - chr1.4:87759543-87759562 None:intergenic 40.0%
ATGAAATTCACTACACGACC+AGG + chr1.4:87760302-87760321 MS.gene90833:intron 40.0%
CAGAAAATTGTACAGCAACC+TGG - chr1.4:87760323-87760342 None:intergenic 40.0%
CGTCCATTTACCAATTCTGA+AGG + chr1.4:87759507-87759526 MS.gene90833:intron 40.0%
GTAAAGCAAGTTCAGCTTTG+AGG - chr1.4:87759321-87759340 None:intergenic 40.0%
! ACCATCCATCAATTGGTGAT+TGG + chr1.4:87759973-87759992 MS.gene90833:intron 40.0%
AGGGTTTAATACCTGGTGCT+TGG - chr1.4:87759819-87759838 None:intergenic 45.0%
AGTGATTTCCCAGAAGCAGA+AGG + chr1.4:87759706-87759725 MS.gene90833:CDS 45.0%
ATACAGATCCTGCAGGTGTA+GGG + chr1.4:87760128-87760147 MS.gene90833:intron 45.0%
CCAAATGATACAGATCCTGC+AGG + chr1.4:87760121-87760140 MS.gene90833:intron 45.0%
CCTGCAGGATCTGTATCATT+TGG - chr1.4:87760124-87760143 None:intergenic 45.0%
TACACCTCCCAATGTATCTG+TGG + chr1.4:87759881-87759900 MS.gene90833:intron 45.0%
! CTCCTTTTTCTTCTCCCTCT+CGG - chr1.4:87760623-87760642 None:intergenic 45.0%
! TGCTTTACTCCGTGTTGCTA+AGG + chr1.4:87759333-87759352 MS.gene90833:CDS 45.0%
!! GGTTTTTCAGTAAGGTGGTG+AGG + chr1.4:87759660-87759679 MS.gene90833:intron 45.0%
CAAAGCAGCCTTCTGCTTCT+GGG - chr1.4:87759717-87759736 None:intergenic 50.0%
CACCAGTGACCTTAGCAACA+CGG - chr1.4:87759345-87759364 None:intergenic 50.0%
CAGTGAAGGTTCACGAGTTG+AGG + chr1.4:87759257-87759276 MS.gene90833:intron 50.0%
GACCGAGAGGGAGAAGAAAA+AGG + chr1.4:87760618-87760637 MS.gene90833:CDS 50.0%
GATACAGATCCTGCAGGTGT+AGG + chr1.4:87760127-87760146 MS.gene90833:intron 50.0%
TCAAAGCAGCCTTCTGCTTC+TGG - chr1.4:87759718-87759737 None:intergenic 50.0%
TTCTTTGGGCGGAGATCAAG+TGG - chr1.4:87759770-87759789 None:intergenic 50.0%
! AGAAGCAGAAGGCTGCTTTG+AGG + chr1.4:87759717-87759736 MS.gene90833:CDS 50.0%
! GAAGCAGAAGGCTGCTTTGA+GGG + chr1.4:87759718-87759737 MS.gene90833:CDS 50.0%
! GAATGGCCCTGGTCTTCTTT+GGG - chr1.4:87759784-87759803 None:intergenic 50.0%
AGTCTTCGCTGAAGACCGAG+AGG + chr1.4:87760605-87760624 MS.gene90833:intron 55.0%
ATCCTGCAGGTGTAGGGAGA+AGG + chr1.4:87760134-87760153 MS.gene90833:intron 55.0%
ATCCTTCTCCCTACACCTGC+AGG - chr1.4:87760139-87760158 None:intergenic 55.0%
ATCTCCGCCCAAAGAAGACC+AGG + chr1.4:87759774-87759793 MS.gene90833:CDS 55.0%
CTTGGTGAGCCTTCTGCGAA+TGG - chr1.4:87759801-87759820 None:intergenic 55.0%
GTCTTCGCTGAAGACCGAGA+GGG + chr1.4:87760606-87760625 MS.gene90833:intron 55.0%
TCTCCGCCCAAAGAAGACCA+GGG + chr1.4:87759775-87759794 MS.gene90833:CDS 55.0%
! CGAATGGCCCTGGTCTTCTT+TGG - chr1.4:87759785-87759804 None:intergenic 55.0%
! CGTGTTGCTAAGGTCACTGG+TGG + chr1.4:87759343-87759362 MS.gene90833:CDS 55.0%
! CTCCGTGTTGCTAAGGTCAC+TGG + chr1.4:87759340-87759359 MS.gene90833:CDS 55.0%
! GGTGAGGTTGTCGATTGCTC+AGG + chr1.4:87759676-87759695 MS.gene90833:CDS 55.0%
AGACCAGGGCCATTCGCAGA+AGG + chr1.4:87759789-87759808 MS.gene90833:CDS 60.0%
CAGAAGGCTCACCAAGCACC+AGG + chr1.4:87759805-87759824 MS.gene90833:CDS 60.0%
! TGGCCCTGGTCTTCTTTGGG+CGG - chr1.4:87759781-87759800 None:intergenic 60.0%
GAGCCTTCTGCGAATGGCCC+TGG - chr1.4:87759795-87759814 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 87759157 87760681 87759157 ID=MS.gene90833
chr1.4 mRNA 87759157 87760681 87759157 ID=MS.gene90833.t1;Parent=MS.gene90833
chr1.4 exon 87759157 87759160 87759157 ID=MS.gene90833.t1.exon1;Parent=MS.gene90833.t1
chr1.4 CDS 87759157 87759160 87759157 ID=cds.MS.gene90833.t1;Parent=MS.gene90833.t1
chr1.4 exon 87759260 87759389 87759260 ID=MS.gene90833.t1.exon2;Parent=MS.gene90833.t1
chr1.4 CDS 87759260 87759389 87759260 ID=cds.MS.gene90833.t1;Parent=MS.gene90833.t1
chr1.4 exon 87759670 87759826 87759670 ID=MS.gene90833.t1.exon3;Parent=MS.gene90833.t1
chr1.4 CDS 87759670 87759826 87759670 ID=cds.MS.gene90833.t1;Parent=MS.gene90833.t1
chr1.4 exon 87760607 87760681 87760607 ID=MS.gene90833.t1.exon4;Parent=MS.gene90833.t1
chr1.4 CDS 87760607 87760681 87760607 ID=cds.MS.gene90833.t1;Parent=MS.gene90833.t1
Gene Sequence

>MS.gene90833

ATGGGTAAATTTCCCTAAACCACCCTAATTTCTGAAATCAAACAAACAATTTCTCATAATCAATCAATCAATTTCACTAATTAGCATGTGTTTTGATTTGCAGTGAAGGTTCACGAGTTGAGGCAGAAAAACAAAGCTGATCTGTTGGCTCAGCTCAAAGACCTCAAAGCTGAACTTGCTTTACTCCGTGTTGCTAAGGTCACTGGTGGTGCACCCAACAAGCTCTCCAAAATGTAAATAAAAATTCAATCTTTATTCTTTTATGTCAATTGGTGCTATGATCGTAAAGGTGTTTTGACGTGCAGTTTTGCTCAACAAAGATCACTTGTTGTTGTGTATGTAATGTATTGCGTCCATTTACCAATTCTGAAGGATAAATTGTTCCTTTCGGTGTAATGGCTTTGATTGTGTGATGATTATAATGGGTTGTTTTTGTTTTGTGTCTGTAAGGTGGTGTTTGTGTTAATGATTGATTGATGTGTTGTTTTTGATTATTTTATTTGGGTTTTTCAGTAAGGTGGTGAGGTTGTCGATTGCTCAGGTGTTGACAGTGATTTCCCAGAAGCAGAAGGCTGCTTTGAGGGAAGTTTACAAGAACAAGAAGTATTTGCCACTTGATCTCCGCCCAAAGAAGACCAGGGCCATTCGCAGAAGGCTCACCAAGCACCAGGTATTAAACCCTTTTAATGTATAATTCATGTTAAAGTTCATTGTTATAAACATATACACCTCCCAATGTATCTGTGGTTTTTGCTTTTGACTGCATTGATGCATTTCATTGGTTTTTTTTTTTTTTTCAATTCATATTTTATTGTGACCATCCATCAATTGGTGATTGGTGTATATTAGCTTTAATGAGATCTCTAATACGATGCTTTAGAGTAATCTGATTTTTGTCTCAAGTACTATTTAGGAGTATGAATGAATGAAATCTTAAATAATGAGGTGCTTCTTGTTCCTTATTCCAAATGATACAGATCCTGCAGGTGTAGGGAGAAGGATTATTGAATTTGACAGTGCTTAGTTTTCTTTCTAAAATCGAAGGATTTCAAGTCGACCTACACCATTAAATGTTATCCTTGAAAGAACAGTAATAATCAGTAGTACCTTAACACATAAAATTGAATGTCAAAATCCATTTTTACATGAAATTCACTACACGACCAGGTTGCTGTACAATTTTCTGTTTGTTTAGAAGATAATAACTAGATTGGTTTATTGTTGGATGCTATTATAGCCTTTTACACCATTGGTTGTTTTCAACCTGATTTGTCTTGGGATATTCAATCTTACATGAGTTTAGTCTTTCCTTGTAACCTTTTTCTCTTTGCTTGTATGTCAATCTCTTCCTATATATACAAACTGAAATTTTCTTGTGTAATGATTACATATTCAAGCAGAACAGTATGTTTGGATTGTTACTTATGATATGTTTTACTTGTTCATGCAGTCTTCGCTGAAGACCGAGAGGGAGAAGAAAAAGGAGACATATTTCCCAATGAGGAAATATGCTATCAAGGCATAG

Protein sequence

>MS.gene90833.t1

MVKVHELRQKNKADLLAQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQSSLKTEREKKKETYFPMRKYAIKA