Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67378.t1 | XP_003606603.1 | 95.8 | 120 | 5 | 0 | 3 | 122 | 4 | 123 | 3.80E-51 | 210.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67378.t1 | Q9M5L0 | 91.6 | 119 | 10 | 0 | 3 | 121 | 4 | 122 | 1.6e-50 | 199.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67378.t1 | G7JTN1 | 95.8 | 120 | 5 | 0 | 3 | 122 | 4 | 123 | 2.8e-51 | 210.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene67378 | MS.gene066455 | PPI |
| MS.gene67378 | MS.gene036455 | PPI |
| MS.gene67378 | MS.gene074269 | PPI |
| MS.gene029109 | MS.gene67378 | PPI |
| MS.gene021047 | MS.gene67378 | PPI |
| MS.gene75171 | MS.gene67378 | PPI |
| MS.gene90833 | MS.gene67378 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67378.t1 | MTR_4g062410 | 95.041 | 121 | 6 | 0 | 2 | 122 | 3 | 123 | 1.55e-76 | 222 |
| MS.gene67378.t1 | MTR_1g067490 | 92.623 | 122 | 9 | 0 | 1 | 122 | 1 | 122 | 2.11e-75 | 219 |
| MS.gene67378.t1 | MTR_4g072410 | 91.597 | 119 | 10 | 0 | 4 | 122 | 3 | 121 | 1.18e-71 | 209 |
| MS.gene67378.t1 | MTR_1g115545 | 90.000 | 120 | 12 | 0 | 3 | 122 | 2 | 121 | 1.95e-71 | 209 |
| MS.gene67378.t1 | MTR_7g080090 | 85.000 | 120 | 18 | 0 | 3 | 122 | 4 | 123 | 2.31e-65 | 194 |
| MS.gene67378.t1 | MTR_1g105880 | 70.787 | 89 | 15 | 1 | 10 | 98 | 2 | 79 | 1.24e-33 | 112 |
| MS.gene67378.t1 | MTR_5g045540 | 85.294 | 34 | 5 | 0 | 67 | 100 | 3 | 36 | 3.05e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67378.t1 | AT3G09500 | 86.555 | 119 | 16 | 0 | 3 | 121 | 4 | 122 | 4.61e-68 | 201 |
| MS.gene67378.t1 | AT5G02610 | 87.395 | 119 | 15 | 0 | 3 | 121 | 4 | 122 | 4.81e-68 | 201 |
| MS.gene67378.t1 | AT2G39390 | 86.555 | 119 | 16 | 0 | 3 | 121 | 4 | 122 | 5.86e-68 | 201 |
| MS.gene67378.t1 | AT3G55170 | 84.034 | 119 | 19 | 0 | 3 | 121 | 4 | 122 | 2.17e-65 | 194 |
| MS.gene67378.t1 | AT3G55170 | 84.034 | 119 | 19 | 0 | 3 | 121 | 4 | 122 | 2.17e-65 | 194 |
| MS.gene67378.t1 | AT3G55170 | 84.034 | 119 | 19 | 0 | 3 | 121 | 4 | 122 | 2.17e-65 | 194 |
| MS.gene67378.t1 | AT5G02610 | 73.239 | 142 | 15 | 1 | 3 | 121 | 4 | 145 | 2.63e-62 | 187 |
| MS.gene67378.t1 | AT3G55170 | 83.505 | 97 | 16 | 0 | 3 | 99 | 4 | 100 | 1.80e-50 | 155 |
| MS.gene67378.t1 | AT3G55170 | 83.505 | 97 | 16 | 0 | 3 | 99 | 4 | 100 | 1.80e-50 | 155 |
Find 27 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAATAGCTCTCGTCTTCT+TGG | 0.242711 | 4.1:-37522711 | None:intergenic |
| AGCATTGAGGGAAGTTTATA+AGG | 0.261646 | 4.1:+37522658 | MS.gene67378:CDS |
| GGTCCAACATTACGCCTTAA+TGG | 0.287059 | 4.1:-37523578 | None:intergenic |
| TACATCTTGGAAAGTTTGTT+AGG | 0.319850 | 4.1:-37522383 | None:intergenic |
| CTAATAGCTCTCGTCTTCTT+GGG | 0.390815 | 4.1:-37522710 | None:intergenic |
| AGTGAGAAAGTATGCCATTA+AGG | 0.406198 | 4.1:+37523564 | MS.gene67378:CDS |
| GCATTGAGGGAAGTTTATAA+GGG | 0.408192 | 4.1:+37522659 | MS.gene67378:CDS |
| GGGGAAGAAGTACTTGCCTT+TGG | 0.408254 | 4.1:+37522679 | MS.gene67378:CDS |
| TTTGAACGCAATAGCTATCA+AGG | 0.421455 | 4.1:+37522256 | MS.gene67378:intron |
| TTGGATGCAGCAAGGTTGTT+CGG | 0.445039 | 4.1:+37522588 | MS.gene67378:intron |
| ACATCTTGGAAAGTTTGTTA+GGG | 0.473499 | 4.1:-37522382 | None:intergenic |
| GAAACAAAAGACAGCATTGA+GGG | 0.506620 | 4.1:+37522646 | MS.gene67378:CDS |
| CTTCGCGTCGCGAAAGTCAC+CGG | 0.510669 | 4.1:+37522353 | MS.gene67378:CDS |
| AAGACGCCTCACTAAGCATC+AGG | 0.512300 | 4.1:+37522733 | MS.gene67378:CDS |
| TTAATGGCATACTTTCTCAC+TGG | 0.517469 | 4.1:-37523562 | None:intergenic |
| AAGTTTGTTAGGGGCACCGC+CGG | 0.537053 | 4.1:-37522372 | None:intergenic |
| TTCTTGGGACGGAGATCCAA+AGG | 0.574336 | 4.1:-37522695 | None:intergenic |
| TAATGGCATACTTTCTCACT+GGG | 0.582481 | 4.1:-37523561 | None:intergenic |
| GACAGAGCGTGAGAAGAAGA+AGG | 0.584517 | 4.1:+37523528 | MS.gene67378:CDS |
| CGGTGACTTTCGCGACGCGA+AGG | 0.585891 | 4.1:-37522352 | None:intergenic |
| TAGCTCTCGTCTTCTTGGGA+CGG | 0.610807 | 4.1:-37522706 | None:intergenic |
| CTATCAAGGTTTATGAGCTG+AGG | 0.615557 | 4.1:+37522270 | MS.gene67378:CDS |
| CATCTTGGAAAGTTTGTTAG+GGG | 0.617458 | 4.1:-37522381 | None:intergenic |
| CATTGAGGGAAGTTTATAAG+GGG | 0.623355 | 4.1:+37522660 | MS.gene67378:CDS |
| TATGTACCTGATGCTTAGTG+AGG | 0.656359 | 4.1:-37522739 | None:intergenic |
| CGCGTCGCGAAAGTCACCGG+CGG | 0.657741 | 4.1:+37522356 | MS.gene67378:CDS |
| AGAAACAAAAGACAGCATTG+AGG | 0.664403 | 4.1:+37522645 | MS.gene67378:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTAGAGAATAGAATAA+AGG | - | chr4.1:37522180-37522199 | None:intergenic | 15.0% |
| !! | ATTATGAAAAAATGAGTAAA+TGG | - | chr4.1:37523038-37523057 | None:intergenic | 15.0% |
| !! | TTTGTTACAATTATAATTGT+GGG | + | chr4.1:37523099-37523118 | MS.gene67378:intron | 15.0% |
| !!! | TACATATTTTAACAATATTG+TGG | + | chr4.1:37522756-37522775 | MS.gene67378:intron | 15.0% |
| !!! | TTTTTTCATAATTGTTTATG+TGG | + | chr4.1:37523046-37523065 | MS.gene67378:intron | 15.0% |
| !! | CTTTGTTACAATTATAATTG+TGG | + | chr4.1:37523098-37523117 | MS.gene67378:intron | 20.0% |
| !! | TATAAGCTTTCTTCATAAAA+TGG | + | chr4.1:37523464-37523483 | MS.gene67378:intron | 20.0% |
| !! | TTGTTACAATTATAATTGTG+GGG | + | chr4.1:37523100-37523119 | MS.gene67378:intron | 20.0% |
| !!! | AATACTTCTTATGATGTTTT+CGG | + | chr4.1:37523391-37523410 | MS.gene67378:intron | 20.0% |
| !!! | TCATCTATAGTAATTTTGAT+TGG | - | chr4.1:37522516-37522535 | None:intergenic | 20.0% |
| !!! | TGATTGTTTTTGTTATTGTT+TGG | + | chr4.1:37522569-37522588 | MS.gene67378:intron | 20.0% |
| ! | AATTAAGTGCAATAGAATAG+CGG | - | chr4.1:37522417-37522436 | None:intergenic | 25.0% |
| ! | ATCAGTACAAAAAATGATGT+TGG | - | chr4.1:37522207-37522226 | None:intergenic | 25.0% |
| ! | TAGAGAATAGAATAAAGGAT+TGG | - | chr4.1:37522175-37522194 | None:intergenic | 25.0% |
| ! | TGAAAAAATGAGTAAATGGT+TGG | - | chr4.1:37523034-37523053 | None:intergenic | 25.0% |
| !! | ATTTTATCAATGTGCCTTAT+TGG | + | chr4.1:37523169-37523188 | MS.gene67378:intron | 25.0% |
| AAAACATGTATGTATGTGCT+CGG | - | chr4.1:37523316-37523335 | None:intergenic | 30.0% | |
| AATGGCTGAATGAATTTACT+TGG | + | chr4.1:37523482-37523501 | MS.gene67378:intron | 30.0% | |
| ATTGTGGAATATAAGAACAG+TGG | + | chr4.1:37522772-37522791 | MS.gene67378:intron | 30.0% | |
| GTGATATATTTGCCTCTTAT+CGG | + | chr4.1:37523199-37523218 | MS.gene67378:intron | 30.0% | |
| GTTTATGTGGACAATACTTA+TGG | + | chr4.1:37523059-37523078 | MS.gene67378:intron | 30.0% | |
| TGATATATTTGCCTCTTATC+GGG | + | chr4.1:37523200-37523219 | MS.gene67378:intron | 30.0% | |
| TTGCGTTCAAAAAAGAAGAA+AGG | - | chr4.1:37522247-37522266 | None:intergenic | 30.0% | |
| ! | TACATCTTGGAAAGTTTGTT+AGG | - | chr4.1:37522386-37522405 | None:intergenic | 30.0% |
| !! | ACATCTTGGAAAGTTTGTTA+GGG | - | chr4.1:37522385-37522404 | None:intergenic | 30.0% |
| !!! | ACATACATGTTTTGACTTCT+AGG | + | chr4.1:37523323-37523342 | MS.gene67378:intron | 30.0% |
| !!! | CATACATGTTTTGACTTCTA+GGG | + | chr4.1:37523324-37523343 | MS.gene67378:intron | 30.0% |
| AAACATGTATGTATGTGCTC+GGG | - | chr4.1:37523315-37523334 | None:intergenic | 35.0% | |
| AAGTTAAAACTCCCGATAAG+AGG | - | chr4.1:37523214-37523233 | None:intergenic | 35.0% | |
| AATTATAATTGTGGGGACTG+GGG | + | chr4.1:37523107-37523126 | MS.gene67378:intron | 35.0% | |
| ACAACTTCACCAACAAAGAT+TGG | - | chr4.1:37523350-37523369 | None:intergenic | 35.0% | |
| ACAATTATAATTGTGGGGAC+TGG | + | chr4.1:37523105-37523124 | MS.gene67378:intron | 35.0% | |
| ACATGTGGTACCATTAAATG+AGG | + | chr4.1:37523007-37523026 | MS.gene67378:intron | 35.0% | |
| AGAAACAAAAGACAGCATTG+AGG | + | chr4.1:37522645-37522664 | MS.gene67378:CDS | 35.0% | |
| AGATTAAGCTTGAACTTGCA+TGG | + | chr4.1:37522973-37522992 | MS.gene67378:intron | 35.0% | |
| ATATCACCTATGCTCCAATA+AGG | - | chr4.1:37523186-37523205 | None:intergenic | 35.0% | |
| CAACCATTCATGAAAAGTCA+AGG | - | chr4.1:37523432-37523451 | None:intergenic | 35.0% | |
| CAATTATAATTGTGGGGACT+GGG | + | chr4.1:37523106-37523125 | MS.gene67378:intron | 35.0% | |
| CATTGAGGGAAGTTTATAAG+GGG | + | chr4.1:37522660-37522679 | MS.gene67378:CDS | 35.0% | |
| GCATTGAGGGAAGTTTATAA+GGG | + | chr4.1:37522659-37522678 | MS.gene67378:CDS | 35.0% | |
| TAATGGCATACTTTCTCACT+GGG | - | chr4.1:37523564-37523583 | None:intergenic | 35.0% | |
| TTAATGGCATACTTTCTCAC+TGG | - | chr4.1:37523565-37523584 | None:intergenic | 35.0% | |
| TTTAATGGTACCACATGTTG+TGG | - | chr4.1:37523005-37523024 | None:intergenic | 35.0% | |
| TTTGAACGCAATAGCTATCA+AGG | + | chr4.1:37522256-37522275 | MS.gene67378:intron | 35.0% | |
| ! | AATGGTTGGTCCTCATTTAA+TGG | - | chr4.1:37523020-37523039 | None:intergenic | 35.0% |
| ! | AGCATTGAGGGAAGTTTATA+AGG | + | chr4.1:37522658-37522677 | MS.gene67378:CDS | 35.0% |
| ! | AGTGAGAAAGTATGCCATTA+AGG | + | chr4.1:37523564-37523583 | MS.gene67378:CDS | 35.0% |
| ! | GAAACAAAAGACAGCATTGA+GGG | + | chr4.1:37522646-37522665 | MS.gene67378:CDS | 35.0% |
| !! | CATCTTGGAAAGTTTGTTAG+GGG | - | chr4.1:37522384-37522403 | None:intergenic | 35.0% |
| !! | CTTAATCTTTGACTTGCTGA+AGG | - | chr4.1:37522961-37522980 | None:intergenic | 35.0% |
| AGCGGAAAATGCATACATCT+TGG | - | chr4.1:37522399-37522418 | None:intergenic | 40.0% | |
| AGCTTGAACTTGCATGGTTT+AGG | + | chr4.1:37522979-37522998 | MS.gene67378:intron | 40.0% | |
| ATTATAATTGTGGGGACTGG+GGG | + | chr4.1:37523108-37523127 | MS.gene67378:intron | 40.0% | |
| CTAATAGCTCTCGTCTTCTT+GGG | - | chr4.1:37522713-37522732 | None:intergenic | 40.0% | |
| CTATCAAGGTTTATGAGCTG+AGG | + | chr4.1:37522270-37522289 | MS.gene67378:CDS | 40.0% | |
| GTGGAAGTAATAGAGGACTT+CGG | - | chr4.1:37522841-37522860 | None:intergenic | 40.0% | |
| GTTATTGTTTGGATGCAGCA+AGG | + | chr4.1:37522580-37522599 | MS.gene67378:intron | 40.0% | |
| TATGTACCTGATGCTTAGTG+AGG | - | chr4.1:37522742-37522761 | None:intergenic | 40.0% | |
| TCTAATAGCTCTCGTCTTCT+TGG | - | chr4.1:37522714-37522733 | None:intergenic | 40.0% | |
| TGGAAGTAATAGAGGACTTC+GGG | - | chr4.1:37522840-37522859 | None:intergenic | 40.0% | |
| ! | AATGTGCCTTATTGGAGCAT+AGG | + | chr4.1:37523177-37523196 | MS.gene67378:intron | 40.0% |
| ! | GTGCCTTGACTTTTCATGAA+TGG | + | chr4.1:37523426-37523445 | MS.gene67378:intron | 40.0% |
| ! | GTTGTAGAGTTTTGCTGCAA+TGG | + | chr4.1:37523142-37523161 | MS.gene67378:intron | 40.0% |
| !! | TTAGGGAAGTGTTTGTCTCA+AGG | - | chr4.1:37522896-37522915 | None:intergenic | 40.0% |
| !! | TTTGACTTGCTGAAGGAATG+TGG | - | chr4.1:37522954-37522973 | None:intergenic | 40.0% |
| AAGTAATAGAGGACTTCGGG+AGG | - | chr4.1:37522837-37522856 | None:intergenic | 45.0% | |
| ATGGTTTAGGCCACAACATG+TGG | + | chr4.1:37522992-37523011 | MS.gene67378:intron | 45.0% | |
| GACAAACACTTCCCTAAGCT+TGG | + | chr4.1:37522899-37522918 | MS.gene67378:intron | 45.0% | |
| GGAGCAAGTGGAAGTAATAG+AGG | - | chr4.1:37522848-37522867 | None:intergenic | 45.0% | |
| TTGGATGCAGCAAGGTTGTT+CGG | + | chr4.1:37522588-37522607 | MS.gene67378:intron | 45.0% | |
| ! | GAAGTGTTTGTCTCAAGGAG+GGG | - | chr4.1:37522891-37522910 | None:intergenic | 45.0% |
| !! | CTTCTAGGGCCAATCTTTGT+TGG | + | chr4.1:37523338-37523357 | MS.gene67378:intron | 45.0% |
| !! | GGAAGTGTTTGTCTCAAGGA+GGG | - | chr4.1:37522892-37522911 | None:intergenic | 45.0% |
| AAGACGCCTCACTAAGCATC+AGG | + | chr4.1:37522733-37522752 | MS.gene67378:CDS | 50.0% | |
| ACTTCCCTAAGCTTGGAACG+TGG | + | chr4.1:37522906-37522925 | MS.gene67378:intron | 50.0% | |
| ATAGAGGACTTCGGGAGGAA+AGG | - | chr4.1:37522832-37522851 | None:intergenic | 50.0% | |
| ATCTGCCACGTTCCAAGCTT+AGG | - | chr4.1:37522914-37522933 | None:intergenic | 50.0% | |
| GACAGAGCGTGAGAAGAAGA+AGG | + | chr4.1:37523528-37523547 | MS.gene67378:CDS | 50.0% | |
| TAGAGGACTTCGGGAGGAAA+GGG | - | chr4.1:37522831-37522850 | None:intergenic | 50.0% | |
| TAGCTCTCGTCTTCTTGGGA+CGG | - | chr4.1:37522709-37522728 | None:intergenic | 50.0% | |
| TCTGCCACGTTCCAAGCTTA+GGG | - | chr4.1:37522913-37522932 | None:intergenic | 50.0% | |
| TGTTTGTCTCAAGGAGGGGA+GGG | - | chr4.1:37522887-37522906 | None:intergenic | 50.0% | |
| TTCTTGGGACGGAGATCCAA+AGG | - | chr4.1:37522698-37522717 | None:intergenic | 50.0% | |
| !! | GGGAAGTGTTTGTCTCAAGG+AGG | - | chr4.1:37522893-37522912 | None:intergenic | 50.0% |
| !! | GGGGAAGAAGTACTTGCCTT+TGG | + | chr4.1:37522679-37522698 | MS.gene67378:CDS | 50.0% |
| GGAAAGGAGGAAGGAGCAAG+TGG | - | chr4.1:37522860-37522879 | None:intergenic | 55.0% | |
| GTGTTTGTCTCAAGGAGGGG+AGG | - | chr4.1:37522888-37522907 | None:intergenic | 55.0% | |
| TTCGGGAGGAAAGGGCTTTG+CGG | - | chr4.1:37522823-37522842 | None:intergenic | 55.0% | |
| TTGTGGGGACTGGGGGAAAT+TGG | + | chr4.1:37523115-37523134 | MS.gene67378:intron | 55.0% | |
| !! | AAGTTTGTTAGGGGCACCGC+CGG | - | chr4.1:37522375-37522394 | None:intergenic | 55.0% |
| CTTCGCGTCGCGAAAGTCAC+CGG | + | chr4.1:37522353-37522372 | MS.gene67378:CDS | 60.0% | |
| GAGGGCTGTGGAAAGGAGGA+AGG | - | chr4.1:37522869-37522888 | None:intergenic | 60.0% | |
| AGGAGGGGAGGGCTGTGGAA+AGG | - | chr4.1:37522876-37522895 | None:intergenic | 65.0% | |
| AGGGGAGGGCTGTGGAAAGG+AGG | - | chr4.1:37522873-37522892 | None:intergenic | 65.0% | |
| CGGTGACTTTCGCGACGCGA+AGG | - | chr4.1:37522355-37522374 | None:intergenic | 65.0% | |
| TCTCAAGGAGGGGAGGGCTG+TGG | - | chr4.1:37522881-37522900 | None:intergenic | 65.0% | |
| CGCGTCGCGAAAGTCACCGG+CGG | + | chr4.1:37522356-37522375 | MS.gene67378:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 37522156 | 37523591 | 37522156 | ID=MS.gene67378 |
| chr4.1 | mRNA | 37522156 | 37523591 | 37522156 | ID=MS.gene67378.t1;Parent=MS.gene67378 |
| chr4.1 | exon | 37522156 | 37522159 | 37522156 | ID=MS.gene67378.t1.exon1;Parent=MS.gene67378.t1 |
| chr4.1 | CDS | 37522156 | 37522159 | 37522156 | ID=cds.MS.gene67378.t1;Parent=MS.gene67378.t1 |
| chr4.1 | exon | 37522270 | 37522402 | 37522270 | ID=MS.gene67378.t1.exon2;Parent=MS.gene67378.t1 |
| chr4.1 | CDS | 37522270 | 37522402 | 37522270 | ID=cds.MS.gene67378.t1;Parent=MS.gene67378.t1 |
| chr4.1 | exon | 37522598 | 37522754 | 37522598 | ID=MS.gene67378.t1.exon3;Parent=MS.gene67378.t1 |
| chr4.1 | CDS | 37522598 | 37522754 | 37522598 | ID=cds.MS.gene67378.t1;Parent=MS.gene67378.t1 |
| chr4.1 | exon | 37523517 | 37523591 | 37523517 | ID=MS.gene67378.t1.exon4;Parent=MS.gene67378.t1 |
| chr4.1 | CDS | 37523517 | 37523591 | 37523517 | ID=cds.MS.gene67378.t1;Parent=MS.gene67378.t1 |
| Gene Sequence |
| Protein sequence |
