Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074499.t1 | XP_003618123.2 | 93.9 | 277 | 17 | 0 | 10 | 286 | 37 | 313 | 1.70E-150 | 542 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074499.t1 | B5X582 | 54.9 | 266 | 109 | 3 | 31 | 286 | 125 | 389 | 5.1e-79 | 295.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074499.t1 | G7KFG5 | 93.9 | 277 | 17 | 0 | 10 | 286 | 32 | 308 | 1.2e-150 | 542.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene074499 | 0.814985 | 1.17E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene074499 | 0.831806 | 1.35E-55 | -1.69E-46 |
| MS.gene050762 | MS.gene074499 | 0.830211 | 3.34E-55 | -1.69E-46 |
| MS.gene050763 | MS.gene074499 | 0.831578 | 1.54E-55 | -1.69E-46 |
| MS.gene052176 | MS.gene074499 | 0.816628 | 5.01E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene074499 | 0.806543 | 7.82E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene074499 | 0.801417 | 9.09E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene074499 | 0.813182 | 2.91E-51 | -1.69E-46 |
| MS.gene054379 | MS.gene074499 | 0.832015 | 1.20E-55 | -1.69E-46 |
| MS.gene054930 | MS.gene074499 | 0.837204 | 5.97E-57 | -1.69E-46 |
| MS.gene060084 | MS.gene074499 | 0.807664 | 4.53E-50 | -1.69E-46 |
| MS.gene060940 | MS.gene074499 | 0.804817 | 1.80E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074499.t1 | MTR_5g099220 | 93.863 | 277 | 17 | 0 | 10 | 286 | 32 | 308 | 0.0 | 533 |
| MS.gene074499.t1 | MTR_5g099220 | 93.841 | 276 | 17 | 0 | 10 | 285 | 37 | 312 | 0.0 | 531 |
| MS.gene074499.t1 | MTR_5g099220 | 93.359 | 256 | 17 | 0 | 10 | 265 | 37 | 292 | 1.98e-176 | 490 |
| MS.gene074499.t1 | MTR_2g031740 | 90.310 | 258 | 25 | 0 | 29 | 286 | 59 | 316 | 3.03e-176 | 489 |
| MS.gene074499.t1 | MTR_5g099220 | 98.729 | 236 | 3 | 0 | 51 | 286 | 1 | 236 | 2.38e-173 | 479 |
| MS.gene074499.t1 | MTR_5g099220 | 98.723 | 235 | 3 | 0 | 51 | 285 | 1 | 235 | 2.62e-172 | 477 |
| MS.gene074499.t1 | MTR_2g031620 | 56.296 | 270 | 103 | 3 | 32 | 286 | 109 | 378 | 1.36e-99 | 306 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074499.t1 | AT1G30680 | 50.847 | 295 | 128 | 4 | 8 | 286 | 96 | 389 | 2.14e-92 | 288 |
| MS.gene074499.t1 | AT1G30660 | 50.498 | 301 | 129 | 5 | 5 | 286 | 8 | 307 | 6.40e-92 | 275 |
| MS.gene074499.t1 | AT1G30660 | 51.568 | 287 | 123 | 4 | 15 | 286 | 19 | 304 | 7.59e-91 | 273 |
| MS.gene074499.t1 | AT1G30660 | 51.568 | 287 | 123 | 4 | 15 | 286 | 29 | 314 | 9.23e-91 | 273 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGCTTGTTGCTTACTTTA+AGG | 0.093113 | 5.4:+78457515 | MS.gene074499:intron |
| GTTATAGACAAGGTCCTTTA+TGG | 0.130566 | 5.4:+78463746 | MS.gene074499:CDS |
| CGCCCCAAACCCAAAGGTTT+TGG | 0.223708 | 5.4:+78455832 | MS.gene074499:CDS |
| GAACAATGGAGAAAAGATTT+TGG | 0.263962 | 5.4:+78463229 | MS.gene074499:CDS |
| GCACCAAAACCTTTGGGTTT+GGG | 0.271135 | 5.4:-78455835 | None:intergenic |
| AAGCTGAGTTCATCTTTCTT+TGG | 0.292938 | 5.4:-78465207 | None:intergenic |
| GGCACCAAAACCTTTGGGTT+TGG | 0.313375 | 5.4:-78455836 | None:intergenic |
| GTGTAAAGGTGGACAGTTAA+TGG | 0.331736 | 5.4:+78456223 | MS.gene074499:intron |
| AAGAGCTAGCACGGCGCCTT+GGG | 0.357506 | 5.4:+78464614 | MS.gene074499:CDS |
| TTGGGGCGAGAAAAGTGATT+TGG | 0.360080 | 5.4:-78455817 | None:intergenic |
| ATGAATGGCACCAAAACCTT+TGG | 0.365791 | 5.4:-78455842 | None:intergenic |
| GAAGAGCTAGCACGGCGCCT+TGG | 0.374758 | 5.4:+78464613 | MS.gene074499:CDS |
| CCTTACAGAATTTCTGCTTA+AGG | 0.380916 | 5.4:-78457559 | None:intergenic |
| AACCCTAAGCTCTCCTCATC+AGG | 0.396006 | 5.4:-78456830 | None:intergenic |
| TACATGTGGTTGGGCTGGTC+AGG | 0.399429 | 5.4:+78456683 | MS.gene074499:CDS |
| GTGGAACAGCAAAGCTTACT+TGG | 0.403923 | 5.4:+78464431 | MS.gene074499:CDS |
| TGAATGGCACCAAAACCTTT+GGG | 0.435245 | 5.4:-78455841 | None:intergenic |
| AAACCTGATGAGGAGAGCTT+AGG | 0.440096 | 5.4:+78456827 | MS.gene074499:CDS |
| ACCTTTCCTGGTGCACCATC+AGG | 0.440161 | 5.4:-78463984 | None:intergenic |
| CACCAAAACCTTTGGGTTTG+GGG | 0.445536 | 5.4:-78455834 | None:intergenic |
| TTAGACAACTCTTGTTTGCC+TGG | 0.447652 | 5.4:+78456081 | MS.gene074499:CDS |
| TTAGGTGGTTCGCATTATCC+TGG | 0.449392 | 5.4:+78464555 | MS.gene074499:intron |
| TTGACCTGGTGGATCATTAT+CGG | 0.454060 | 5.4:-78464581 | None:intergenic |
| AAACAAAATGGGTTGATTGT+TGG | 0.461337 | 5.4:+78463195 | MS.gene074499:CDS |
| GTTGTAGTGAATTTGCAATC+TGG | 0.465654 | 5.4:+78456646 | MS.gene074499:intron |
| AACCTGATGAGGAGAGCTTA+GGG | 0.465798 | 5.4:+78456828 | MS.gene074499:CDS |
| TAATGTCGTCAAGTCCATAA+AGG | 0.465841 | 5.4:-78463760 | None:intergenic |
| TTCTGCAGATGACAAGGAAC+TGG | 0.466614 | 5.4:+78456799 | MS.gene074499:CDS |
| AAGCTTTCACTTGAAGAGGC+TGG | 0.467773 | 5.4:+78463939 | MS.gene074499:CDS |
| TGATAAGCTTTCACTTGAAG+AGG | 0.469616 | 5.4:+78463935 | MS.gene074499:CDS |
| CACGGCGCCTTGGGCGAGAA+AGG | 0.471934 | 5.4:+78464623 | MS.gene074499:CDS |
| GATAATGATAATAGGTTGAG+GGG | 0.483543 | 5.4:+78463907 | MS.gene074499:intron |
| AGACCTACATGTGGTTGGGC+TGG | 0.487821 | 5.4:+78456678 | MS.gene074499:CDS |
| CAGCAAAGCTTACTTGGATA+AGG | 0.491598 | 5.4:+78464437 | MS.gene074499:CDS |
| GTTGGAACCACTTGGTCCTA+AGG | 0.491634 | 5.4:+78456850 | MS.gene074499:CDS |
| AGTAAGCTTTGCTGTTCCAC+AGG | 0.494786 | 5.4:-78464428 | None:intergenic |
| TGGATACCTTTGGACAAAAG+AGG | 0.502264 | 5.4:-78456116 | None:intergenic |
| AGATCTGCCACCAATAGAAA+AGG | 0.504280 | 5.4:+78464016 | MS.gene074499:CDS |
| CCTTTGGACAAAAGAGGTTG+TGG | 0.510616 | 5.4:-78456110 | None:intergenic |
| TGATAATGATAATAGGTTGA+GGG | 0.514143 | 5.4:+78463906 | MS.gene074499:intron |
| AGCTTAGGGTTGGAACCACT+TGG | 0.515652 | 5.4:+78456842 | MS.gene074499:CDS |
| ACGTACGTACGTACCTTTGG+TGG | 0.517683 | 5.4:-78455874 | None:intergenic |
| AAGGCAACTCTCGGACGAAA+AGG | 0.520966 | 5.4:+78457578 | MS.gene074499:CDS |
| GTGGATCATTATCGGTAGCC+AGG | 0.524131 | 5.4:-78464573 | None:intergenic |
| AGTGTTCCTGATGGTGCACC+AGG | 0.529108 | 5.4:+78463978 | MS.gene074499:CDS |
| CCTTAAGCAGAAATTCTGTA+AGG | 0.535293 | 5.4:+78457559 | MS.gene074499:CDS |
| TCCTGATGGTGCACCAGGAA+AGG | 0.537409 | 5.4:+78463983 | MS.gene074499:CDS |
| CTTCTTCAAAGATGCAAATG+AGG | 0.541918 | 5.4:+78465227 | MS.gene074499:CDS |
| TACGTACCTTTGGTGGAAAG+TGG | 0.559186 | 5.4:-78455867 | None:intergenic |
| TTGATATGCCAGTGTAAAGG+TGG | 0.562981 | 5.4:+78456212 | MS.gene074499:intron |
| CTTTAGCTGAAGAGCTAGCA+CGG | 0.564457 | 5.4:+78464605 | MS.gene074499:CDS |
| TAGACAACTCTTGTTTGCCT+GGG | 0.566831 | 5.4:+78456082 | MS.gene074499:CDS |
| TCATTACCACTTTCCACCAA+AGG | 0.570913 | 5.4:+78455861 | MS.gene074499:CDS |
| AAGAGGTTGTGGTAGTTCCC+AGG | 0.571323 | 5.4:-78456099 | None:intergenic |
| TGATGAGGAGAGCTTAGGGT+TGG | 0.580112 | 5.4:+78456832 | MS.gene074499:CDS |
| TGAATGACCTTTCTCGCCCA+AGG | 0.584678 | 5.4:-78464630 | None:intergenic |
| TCAGGTGTTGGCAAGTACGC+TGG | 0.585250 | 5.4:+78465184 | MS.gene074499:intron |
| TCAGCTAAAGCTTGACCTGG+TGG | 0.586602 | 5.4:-78464592 | None:intergenic |
| GAGAAAAGTGATTTGGTGAG+AGG | 0.600510 | 5.4:-78455810 | None:intergenic |
| ATAGGTTTCTGCAGATGACA+AGG | 0.602576 | 5.4:+78456793 | MS.gene074499:intron |
| TCTTCAGCTAAAGCTTGACC+TGG | 0.612094 | 5.4:-78464595 | None:intergenic |
| AAATTCTGTAAGGCAACTCT+CGG | 0.613403 | 5.4:+78457569 | MS.gene074499:CDS |
| AAGGTGGACAGTTAATGGAG+AGG | 0.614763 | 5.4:+78456228 | MS.gene074499:CDS |
| TGGTGGAAAGTGGTAATGAA+TGG | 0.627860 | 5.4:-78455857 | None:intergenic |
| TTGAATACCTTAGGACCAAG+TGG | 0.632809 | 5.4:-78456857 | None:intergenic |
| TGACCAGCCCAACCACATGT+AGG | 0.639589 | 5.4:-78456681 | None:intergenic |
| TGGTTGCAAGTACAGAACAA+TGG | 0.639955 | 5.4:+78463215 | MS.gene074499:CDS |
| ATAATGATAATAGGTTGAGG+GGG | 0.644644 | 5.4:+78463908 | MS.gene074499:intron |
| AATTGTGTTAGTGTTCCTGA+TGG | 0.656404 | 5.4:+78463969 | MS.gene074499:CDS |
| ACTGGACAATAAACCTGATG+AGG | 0.661447 | 5.4:+78456817 | MS.gene074499:CDS |
| ATACTGCATATCAATACCTG+TGG | 0.667697 | 5.4:+78464412 | MS.gene074499:CDS |
| GCTACCGATAATGATCCACC+AGG | 0.671318 | 5.4:+78464577 | MS.gene074499:CDS |
| GTAGTGAATTTGCAATCTGG+AGG | 0.685376 | 5.4:+78456649 | MS.gene074499:intron |
| ACAGGGAAAAGTTATAGACA+AGG | 0.702128 | 5.4:+78463736 | MS.gene074499:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 78455803 | 78465254 | 78455803 | ID=MS.gene074499 |
| chr5.4 | mRNA | 78455803 | 78465254 | 78455803 | ID=MS.gene074499.t1;Parent=MS.gene074499 |
| chr5.4 | exon | 78455803 | 78455882 | 78455803 | ID=MS.gene074499.t1.exon1;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78455803 | 78455882 | 78455803 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78456080 | 78456131 | 78456080 | ID=MS.gene074499.t1.exon2;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78456080 | 78456131 | 78456080 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78456224 | 78456279 | 78456224 | ID=MS.gene074499.t1.exon3;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78456224 | 78456279 | 78456224 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78456653 | 78456704 | 78456653 | ID=MS.gene074499.t1.exon4;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78456653 | 78456704 | 78456653 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78456797 | 78456871 | 78456797 | ID=MS.gene074499.t1.exon5;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78456797 | 78456871 | 78456797 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78457519 | 78457599 | 78457519 | ID=MS.gene074499.t1.exon6;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78457519 | 78457599 | 78457519 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78463174 | 78463254 | 78463174 | ID=MS.gene074499.t1.exon7;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78463174 | 78463254 | 78463174 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78463740 | 78463805 | 78463740 | ID=MS.gene074499.t1.exon8;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78463740 | 78463805 | 78463740 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78463921 | 78464037 | 78463921 | ID=MS.gene074499.t1.exon9;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78463921 | 78464037 | 78463921 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78464411 | 78464458 | 78464411 | ID=MS.gene074499.t1.exon10;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78464411 | 78464458 | 78464411 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78464559 | 78464644 | 78464559 | ID=MS.gene074499.t1.exon11;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78464559 | 78464644 | 78464559 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| chr5.4 | exon | 78465188 | 78465254 | 78465188 | ID=MS.gene074499.t1.exon12;Parent=MS.gene074499.t1 |
| chr5.4 | CDS | 78465188 | 78465254 | 78465188 | ID=cds.MS.gene074499.t1;Parent=MS.gene074499.t1 |
| Gene Sequence |
| Protein sequence |
