AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060940.t1 MTR_4g121940 96.296 216 8 0 1 216 16 231 1.42e-158 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060940.t1 AT3G16990 61.395 215 83 0 1 215 6 220 1.17e-99 288

Find 0 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTATATTAATAGAGATG+TGG - 32442:15140-15159 MS.gene060940:intron 20.0%
!! TCAAATAAGTCAATTTAAAC+AGG + 32442:15012-15031 None:intergenic 20.0%
!!! AGTTGTTTTTCCATATAAAA+GGG - 32442:15077-15096 MS.gene060940:intron 20.0%
!!! TCAATTTATTTCAATTTTGC+AGG - 32442:14650-14669 MS.gene060940:CDS 20.0%
!!! TTCATAATGAGTTCTAATAA+CGG + 32442:14930-14949 None:intergenic 20.0%
! ACTCATTATGAAGGTTTATT+TGG - 32442:14938-14957 MS.gene060940:intron 25.0%
! ATACATGCTCATAATTATCT+AGG + 32442:15506-15525 None:intergenic 25.0%
! CTCATTATGAAGGTTTATTT+GGG - 32442:14939-14958 MS.gene060940:intron 25.0%
! GTTATTAGAACTCATTATGA+AGG - 32442:14929-14948 MS.gene060940:intron 25.0%
! TACATGCTCATAATTATCTA+GGG + 32442:15505-15524 None:intergenic 25.0%
! TCATTATGAAGGTTTATTTG+GGG - 32442:14940-14959 MS.gene060940:intron 25.0%
!! TAGTTTTTGTTAGGTTTTTG+AGG + 32442:14864-14883 None:intergenic 25.0%
!!! CAGTTGTTTTTCCATATAAA+AGG - 32442:15076-15095 MS.gene060940:intron 25.0%
AACCTAACAAAAACTATTGC+AGG - 32442:14868-14887 MS.gene060940:intron 30.0%
AATCTCATTCCTTCAAAACA+TGG - 32442:14572-14591 MS.gene060940:CDS 30.0%
ACATGCTCATAATTATCTAG+GGG + 32442:15504-15523 None:intergenic 30.0%
ACTAACAAAGTCTACATTCA+GGG - 32442:15402-15421 MS.gene060940:intron 30.0%
CTCTCAAGGATGAAATATTA+TGG - 32442:14790-14809 MS.gene060940:intron 30.0%
GATGATGTGGAAGTTATATT+GGG - 32442:14756-14775 MS.gene060940:CDS 30.0%
TTGAACATGAAGTTCATTTC+TGG - 32442:15806-15825 MS.gene060940:CDS 30.0%
TTGCAATTTATCTGCATTCA+GGG - 32442:15431-15450 MS.gene060940:intron 30.0%
TTGCAATTTCTCTTCATTCA+GGG + 32442:15281-15300 None:intergenic 30.0%
TTTGAAGGAATGAGATTGAA+CGG + 32442:14569-14588 None:intergenic 30.0%
TTTGACCTTATCTACTGTAT+AGG - 32442:15027-15046 MS.gene060940:intron 30.0%
TTTGCAATTTATCTGCATTC+AGG - 32442:15430-15449 MS.gene060940:intron 30.0%
TTTGCAATTTCTCTTCATTC+AGG + 32442:15282-15301 None:intergenic 30.0%
! TACCTGCAATAGTTTTTGTT+AGG + 32442:14873-14892 None:intergenic 30.0%
! TGATGATGTGGAAGTTATAT+TGG - 32442:14755-14774 MS.gene060940:CDS 30.0%
!!! TCTAGTTTTTGCTAGTATAC+CGG - 32442:14982-15001 MS.gene060940:intron 30.0%
AAAAACACCACCAGAATTGA+AGG - 32442:15651-15670 MS.gene060940:intron 35.0%
AAGTTTGATGAGTCAAGATG+TGG - 32442:15546-15565 MS.gene060940:CDS 35.0%
ACTAACGAAGTTTGCATTCA+GGG - 32442:15338-15357 MS.gene060940:intron 35.0%
GACTAACAAAGTCTACATTC+AGG - 32442:15401-15420 MS.gene060940:intron 35.0%
GTTCTCCTATACAGTAGATA+AGG + 32442:15035-15054 None:intergenic 35.0%
TAAGAGAGAAGCCAACAAAT+GGG - 32442:14815-14834 MS.gene060940:intron 35.0%
TTAAGAGAGAAGCCAACAAA+TGG - 32442:14814-14833 MS.gene060940:intron 35.0%
TTTGCACACTGTATTGAAGA+AGG - 32442:15625-15644 MS.gene060940:CDS 35.0%
! AATGAGTTCTAATAACGGCA+TGG + 32442:14925-14944 None:intergenic 35.0%
! GTTACACACACCCTTTTATA+TGG + 32442:15090-15109 None:intergenic 35.0%
! TATTTCAATTTTGCAGGCTC+AGG - 32442:14656-14675 MS.gene060940:CDS 35.0%
! TGACTATCTTTTGCAGAGAT+TGG + 32442:15720-15739 None:intergenic 35.0%
! TTCTGATGATGAGCTAAAGA+AGG - 32442:15759-15778 MS.gene060940:CDS 35.0%
!! AGTCATTTTAGTCCCTGAAT+GGG - 32442:15235-15254 MS.gene060940:intron 35.0%
!! TAGTCATTTTAGTCCCTGAA+TGG - 32442:15234-15253 MS.gene060940:intron 35.0%
AAGAGAGAAGCCAACAAATG+GGG - 32442:14816-14835 MS.gene060940:intron 40.0%
AAGGAGACTTGTGAAAGATG+GGG - 32442:15670-15689 MS.gene060940:intron 40.0%
ATCAGCACACTAGCAACAAA+TGG + 32442:14708-14727 None:intergenic 40.0%
CACGGATGGAGAGAATAAAA+GGG + 32442:14540-14559 None:intergenic 40.0%
GAAGGAGACTTGTGAAAGAT+GGG - 32442:15669-15688 MS.gene060940:intron 40.0%
GACTAACGAAGTTTGCATTC+AGG - 32442:15337-15356 MS.gene060940:intron 40.0%
GAGTGAAATACCCCATTTGT+TGG + 32442:14829-14848 None:intergenic 40.0%
GATGTGGAAGTTATATTGGG+AGG - 32442:14759-14778 MS.gene060940:CDS 40.0%
GGAAGTTATATTGGGAGGTA+TGG - 32442:14764-14783 MS.gene060940:CDS 40.0%
GTCAATTTAAACAGGCCTAC+CGG + 32442:15004-15023 None:intergenic 40.0%
TCACAAGTCTCCTTCAATTC+TGG + 32442:15664-15683 None:intergenic 40.0%
TCACGGATGGAGAGAATAAA+AGG + 32442:14541-14560 None:intergenic 40.0%
TGAAGGAGACTTGTGAAAGA+TGG - 32442:15668-15687 MS.gene060940:intron 40.0%
TGAGCATGTATTGTTGTGAC+AGG - 32442:15515-15534 MS.gene060940:CDS 40.0%
TTTATTCTCTCCATCCGTGA+TGG - 32442:14541-14560 MS.gene060940:CDS 40.0%
! AGTCACTTTAGTCCCTGAAT+CGG + 32442:15477-15496 None:intergenic 40.0%
! ATCAGATGACAGTGATGATG+TGG - 32442:14743-14762 MS.gene060940:CDS 40.0%
! GTTTTTGCTAGTATACCGGT+AGG - 32442:14986-15005 MS.gene060940:intron 40.0%
! TCAAGATGTGGATTATACCG+TGG - 32442:15558-15577 MS.gene060940:CDS 40.0%
! TGCATTTTGGGCAATTGAAG+TGG - 32442:15588-15607 MS.gene060940:CDS 40.0%
!! ACTTACGAGCCATGTTTTGA+AGG + 32442:14584-14603 None:intergenic 40.0%
!! TGTGCTGATTAAAGCTTGCA+AGG - 32442:14719-14738 MS.gene060940:CDS 40.0%
ACTTTAGTCCCTGAATCGGA+AGG + 32442:15473-15492 None:intergenic 45.0%
AGTCAATCAACGCTTGCAGA+AGG - 32442:15735-15754 MS.gene060940:intron 45.0%
ATTGCAAAACAGTCCCTGCA+TGG - 32442:15294-15313 MS.gene060940:intron 45.0%
CAAGTCTCCTTCAATTCTGG+TGG + 32442:15661-15680 None:intergenic 45.0%
CCAAAATGCAGTAAGAGCCA+CGG + 32442:15578-15597 None:intergenic 45.0%
CTTTAGTCCCTGAATCGGAA+GGG + 32442:15472-15491 None:intergenic 45.0%
GACGAATCATCTCCCATTCA+GGG + 32442:15250-15269 None:intergenic 45.0%
GTATTGTTGTGACAGGTTGC+TGG - 32442:15522-15541 MS.gene060940:CDS 45.0%
GTGAAAGATGGGGCAATGAA+GGG - 32442:15680-15699 MS.gene060940:intron 45.0%
TCCCTGAAATTGACCATGCA+GGG + 32442:15310-15329 None:intergenic 45.0%
TCCCTGCATGGTCAATTTCA+GGG - 32442:15306-15325 MS.gene060940:intron 45.0%
TGACGAATCATCTCCCATTC+AGG + 32442:15251-15270 None:intergenic 45.0%
TGTGAAAGATGGGGCAATGA+AGG - 32442:15679-15698 MS.gene060940:intron 45.0%
! CGTGGCTCTTACTGCATTTT+GGG - 32442:15576-15595 MS.gene060940:CDS 45.0%
!! CATTTCTGGAATATGAGCCG+TGG - 32442:15820-15839 MS.gene060940:CDS 45.0%
!! GGAGAGAATAAAAGGGTGTC+TGG + 32442:14533-14552 None:intergenic 45.0%
AGATGGGGCAATGAAGGGTT+TGG - 32442:15685-15704 MS.gene060940:intron 50.0%
GACGAATCCCTTCCGATTCA+GGG - 32442:15462-15481 MS.gene060940:CDS 50.0%
GTCCCTGAAATTGACCATGC+AGG + 32442:15311-15330 None:intergenic 50.0%
GTCCCTGCATGGTCAATTTC+AGG - 32442:15305-15324 MS.gene060940:intron 50.0%
TGACGAATCCCTTCCGATTC+AGG - 32442:15461-15480 MS.gene060940:CDS 50.0%
TTGCTGCGTCGTAGATAGGA+CGG + 32442:14510-14529 None:intergenic 50.0%
! CCGTGGCTCTTACTGCATTT+TGG - 32442:15575-15594 MS.gene060940:CDS 50.0%
!! GAGATTGAACGGTGCCATCA+CGG + 32442:14558-14577 None:intergenic 50.0%
CTGGTTGCTGCGTCGTAGAT+AGG + 32442:14514-14533 None:intergenic 55.0%
! GGGAGGTATGGCTTCTCTCA+AGG - 32442:14776-14795 MS.gene060940:CDS 55.0%
!! TTGAACGGTGCCATCACGGA+TGG + 32442:14554-14573 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
32442 gene 14481 15852 14481 ID=MS.gene060940
32442 mRNA 14481 15852 14481 ID=MS.gene060940.t1;Parent=MS.gene060940
32442 exon 15736 15852 15736 ID=MS.gene060940.t1.exon1;Parent=MS.gene060940.t1
32442 CDS 15736 15852 15736 ID=cds.MS.gene060940.t1;Parent=MS.gene060940.t1
32442 exon 15444 15661 15444 ID=MS.gene060940.t1.exon2;Parent=MS.gene060940.t1
32442 CDS 15444 15661 15444 ID=cds.MS.gene060940.t1;Parent=MS.gene060940.t1
32442 exon 14481 14796 14481 ID=MS.gene060940.t1.exon3;Parent=MS.gene060940.t1
32442 CDS 14481 14796 14481 ID=cds.MS.gene060940.t1;Parent=MS.gene060940.t1
Gene Sequence

>MS.gene060940

ATGACAGAAACATGGTTGAGGAAACACCGTCCTATCTACGACGCAGCAACCAGACACCCTTTTATTCTCTCCATCCGTGATGGCACCGTTCAATCTCATTCCTTCAAAACATGGCTCGTAAGTAATTTTCAATACATCTCTCTCTTCTCTTTTTATTAGTATCATTTTTTCAATTTATTTCAATTTTGCAGGCTCAGGATTACTTGTTTGTTAGAGAGTTTGTTCCATTTGTTGCTAGTGTGCTGATTAAAGCTTGCAAGGAATCAGATGACAGTGATGATGTGGAAGTTATATTGGGAGGTATGGCTTCTCTCAAGGATGAAATATTATGGTTTAAGAGAGAAGCCAACAAATGGGGTATTTCACTCTCAGACGTTGTTCCTCAAAAACCTAACAAAAACTATTGCAGGTATGTTTATTAGTCATCGATTTTTGTTCTAACCATGCCGTTATTAGAACTCATTATGAAGGTTTATTTGGGGTTTTAAATCATCTGTTGTTTCTAGTTTTTGCTAGTATACCGGTAGGCCTGTTTAAATTGACTTATTTGACCTTATCTACTGTATAGGAGAACTTATGAAGACATGTTCAATTTCAGTTGTTTTTCCATATAAAAGGGTGTGTGTAACATTTCTTATAACTATAATATAATTTCGATACAATTATATTAATAGAGATGTGGTAGTAATTTTTTTACATTGACTGCAAACAAGAAGTCTTAATTTTATTTTAAGTGTTTAAAGTAGATAGAATTAGTCATTTTAGTCCCTGAATGGGAGATGATTCGTCACTTTAGTCCCTGAATGAAGAGAAATTGCAAAACAGTCCCTGCATGGTCAATTTCAGGGACTAAATTGACTAACGAAGTTTGCATTCAGGGACTAAATTGACTAACGAAGTTTGCATTCAGGGACTAAATTGACTAACAAAGTCTACATTCAGGGACTATTTTGCAATTTATCTGCATTCAGGGACTAAAGTGACGAATCCCTTCCGATTCAGGGACTAAAGTGACTAATTCCCCTAGATAATTATGAGCATGTATTGTTGTGACAGGTTGCTGGAAAGTTTGATGAGTCAAGATGTGGATTATACCGTGGCTCTTACTGCATTTTGGGCAATTGAAGTGGTGTATCAAGAGAGTTTTGCACACTGTATTGAAGAAGGCTCAAAAACACCACCAGAATTGAAGGAGACTTGTGAAAGATGGGGCAATGAAGGGTTTGGTCAGTACTGCCAATCTCTGCAAAAGATAGTCAATCAACGCTTGCAGAAGGCTTCTGATGATGAGCTAAAGAAGGCTGAAGTCATGTTGCTGAGTATTATTGAACATGAAGTTCATTTCTGGAATATGAGCCGTGGAAATGTTTGA

Protein sequence

>MS.gene060940.t1

MTETWLRKHRPIYDAATRHPFILSIRDGTVQSHSFKTWLAQDYLFVREFVPFVASVLIKACKESDDSDDVEVILGGMASLKDEILWFKREANKWGISLSDVVPQKPNKNYCRLLESLMSQDVDYTVALTAFWAIEVVYQESFAHCIEEGSKTPPELKETCERWGNEGFGQYCQSLQKIVNQRLQKASDDELKKAEVMLLSIIEHEVHFWNMSRGNV