AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07830.t1 MTR_8g018430 94.541 861 47 0 1 861 1 861 0.0 1700
MS.gene07830.t1 MTR_8g018520 82.907 860 143 1 6 861 9 868 0.0 1487
MS.gene07830.t1 MTR_8g018510 81.640 866 152 3 2 861 5 869 0.0 1484
MS.gene07830.t1 MTR_8g018570 79.814 862 168 3 6 861 9 870 0.0 1456
MS.gene07830.t1 MTR_8g018550 80.581 860 163 1 6 861 9 868 0.0 1446
MS.gene07830.t1 MTR_8g020990 77.791 860 187 1 6 861 9 868 0.0 1423
MS.gene07830.t1 MTR_8g018690 77.146 862 186 2 6 861 9 865 0.0 1402
MS.gene07830.t1 MTR_8g018690 74.942 862 185 3 6 861 9 845 0.0 1352
MS.gene07830.t1 MTR_8g018450 76.525 869 155 8 1 861 1 828 0.0 1345
MS.gene07830.t1 MTR_2g099570 68.881 858 260 4 6 861 9 861 0.0 1249
MS.gene07830.t1 MTR_8g018420 67.330 854 274 2 9 861 7 856 0.0 1224
MS.gene07830.t1 MTR_2g099560 64.203 866 302 4 1 861 1 863 0.0 1176
MS.gene07830.t1 MTR_5g024020 64.311 863 293 7 9 861 10 867 0.0 1168
MS.gene07830.t1 MTR_8g018730 61.877 863 319 6 6 861 5 864 0.0 1142
MS.gene07830.t1 MTR_8g018735 61.910 869 319 6 1 861 1 865 0.0 1139
MS.gene07830.t1 MTR_3g081130 61.189 858 327 5 6 861 5 858 0.0 1120
MS.gene07830.t1 MTR_8g018730 60.441 862 303 6 6 861 5 834 0.0 1106
MS.gene07830.t1 MTR_8g018620 63.700 854 252 10 11 861 9 807 0.0 1105
MS.gene07830.t1 MTR_5g024020 61.761 863 279 8 9 861 10 831 0.0 1103
MS.gene07830.t1 MTR_8g018650 62.837 853 261 8 11 861 9 807 0.0 1099
MS.gene07830.t1 MTR_3g081130 62.611 789 289 5 75 861 2 786 0.0 1055
MS.gene07830.t1 MTR_8g018590 62.409 822 264 10 55 861 32 823 0.0 1042
MS.gene07830.t1 MTR_1g083020 57.816 870 337 8 2 861 9 858 0.0 1008
MS.gene07830.t1 MTR_7g113410 56.431 863 344 9 12 861 24 867 0.0 989
MS.gene07830.t1 MTR_1g104650 55.133 867 353 9 12 861 9 856 0.0 983
MS.gene07830.t1 MTR_8g018730 63.993 586 208 3 277 861 1 584 0.0 812
MS.gene07830.t1 MTR_8g018735 60.356 618 234 5 1 610 1 615 0.0 783
MS.gene07830.t1 MTR_8g020970 44.945 821 435 11 50 860 113 926 0.0 713
MS.gene07830.t1 MTR_8g021020 80.446 404 75 1 462 861 28 431 0.0 694
MS.gene07830.t1 MTR_4g066170 41.183 879 473 21 6 861 49 906 0.0 643
MS.gene07830.t1 MTR_3g479460 42.754 828 432 15 54 861 98 903 0.0 640
MS.gene07830.t1 MTR_8g080230 42.112 824 442 15 57 861 109 916 0.0 635
MS.gene07830.t1 MTR_3g079450 43.686 776 416 13 98 861 136 902 0.0 630
MS.gene07830.t1 MTR_4g088810 42.417 811 427 13 60 860 114 894 0.0 628
MS.gene07830.t1 MTR_8g011340 40.949 801 428 14 49 822 46 828 0.0 606
MS.gene07830.t1 MTR_3g079420 41.667 864 455 18 21 861 28 865 0.0 596
MS.gene07830.t1 MTR_8g020970 43.608 571 305 11 50 610 113 676 4.02e-148 452
MS.gene07830.t1 MTR_6g452500 39.739 614 331 11 250 844 12 605 1.75e-145 443
MS.gene07830.t1 MTR_3g079450 44.359 523 273 11 98 610 136 650 1.82e-134 416
MS.gene07830.t1 MTR_7g012030 62.393 117 43 1 515 630 87 203 4.23e-42 153
MS.gene07830.t1 MTR_6g046330 56.250 128 44 2 515 630 77 204 5.59e-37 142
MS.gene07830.t1 MTR_8g463810 30.370 270 114 6 378 646 161 357 2.56e-30 125
MS.gene07830.t1 MTR_4g088805 39.560 91 55 0 770 860 1 91 2.42e-16 75.5
MS.gene07830.t1 MTR_2g005920 51.786 56 27 0 734 789 58 113 4.41e-13 67.0
MS.gene07830.t1 MTR_7g113408 44.444 63 34 1 753 815 8 69 2.17e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07830.t1 AT1G55020 54.104 865 362 9 9 861 18 859 0.0 976
MS.gene07830.t1 AT3G22400 54.348 874 359 12 9 861 32 886 0.0 967
MS.gene07830.t1 AT1G72520 43.605 821 446 10 50 861 114 926 0.0 706
MS.gene07830.t1 AT1G17420 43.447 824 442 11 50 861 108 919 0.0 698
MS.gene07830.t1 AT3G45140 41.893 845 446 19 35 861 79 896 0.0 621
MS.gene07830.t1 AT3G45140 43.620 768 399 17 111 861 4 754 0.0 609
MS.gene07830.t1 AT1G67560 41.233 827 458 15 50 861 104 917 0.0 608

Find 184 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATGCAGCTGTTAATTT+TGG 0.155837 8.3:+72156518 MS.gene07830:CDS
ATTCTGAACTCCAAGCATTT+TGG 0.213611 8.3:+72156378 MS.gene07830:CDS
CTATAATGGTCAAGTCAATT+AGG 0.235454 8.3:-72156685 None:intergenic
GTTACCTCCGCACCGATTCT+AGG 0.266335 8.3:+72153650 MS.gene07830:CDS
TCACTGTCCCCTTCACCTTT+TGG 0.268716 8.3:-72152789 None:intergenic
TGCCTATCTAGCTTGCTCTT+TGG 0.284977 8.3:-72155119 None:intergenic
TTAGAGGCTTTCAAGAGATT+TGG 0.301087 8.3:+72156791 MS.gene07830:CDS
AGCTTAGCTCGTCCCGTTAT+TGG 0.301482 8.3:+72154134 MS.gene07830:CDS
CTGCAGATGGATTAGAGATA+TGG 0.306273 8.3:+72156285 MS.gene07830:CDS
CCATATCTGAGGAAAATAAA+TGG 0.313419 8.3:+72155384 MS.gene07830:CDS
AGAGAATTGAAGGTGATCTT+TGG 0.324769 8.3:+72156750 MS.gene07830:CDS
TTCTGATTCACAACCATTAG+AGG 0.338910 8.3:+72156775 MS.gene07830:CDS
ATCTAGAGGTGTGGCTCTTA+AGG 0.350357 8.3:+72156211 MS.gene07830:intron
CTGTTCTTTGGTTATTGTGC+TGG 0.354337 8.3:-72155159 None:intergenic
ACAGGTGGATTCAAGGTTCT+TGG 0.357260 8.3:+72152868 MS.gene07830:CDS
ATGGCGTTGAAGCTTCAATT+TGG 0.357268 8.3:+72155553 MS.gene07830:CDS
TCACATTATCAAAAGAATTC+AGG 0.358460 8.3:+72154964 MS.gene07830:CDS
TGCTGTATTATATAAGGATT+GGG 0.361705 8.3:+72155921 MS.gene07830:CDS
ATTGTCTGCTGTATTATATA+AGG 0.364894 8.3:+72155915 MS.gene07830:CDS
TGACACCTACGGTTCAATCT+TGG 0.367124 8.3:+72152912 MS.gene07830:CDS
AAAGTATACGGCATCTTTAC+CGG 0.367235 8.3:-72156887 None:intergenic
CTTCCAAGAGATGAATCATT+TGG 0.368825 8.3:+72154526 MS.gene07830:CDS
AGTTCCTTGAACAATGGTAT+AGG 0.379627 8.3:-72154733 None:intergenic
TGAGTTGCAAACAAAGTTGA+TGG 0.379958 8.3:-72153813 None:intergenic
CATTCATCATAGCAACAAAT+AGG 0.380660 8.3:+72155746 MS.gene07830:CDS
ACACTTGCACCACCCTCATA+TGG 0.380883 8.3:+72156483 MS.gene07830:CDS
GACACCTACGGTTCAATCTT+GGG 0.384321 8.3:+72152913 MS.gene07830:CDS
TGACCAAATGATTCATCTCT+TGG 0.388343 8.3:-72154529 None:intergenic
GGAACAATGATGAGACTCTA+AGG 0.391890 8.3:+72156852 MS.gene07830:CDS
CAAGAGATTTGGAACAAAGT+TGG 0.394650 8.3:+72156802 MS.gene07830:CDS
GCTTCAACGCCATCACTTGC+AGG 0.395161 8.3:-72155543 None:intergenic
CTGCTGTATTATATAAGGAT+TGG 0.398026 8.3:+72155920 MS.gene07830:CDS
ATCAAGTATGTACAATTTGT+TGG 0.404374 8.3:-72155343 None:intergenic
TTTAGGTGGTGGAAACTTAA+GGG 0.405519 8.3:-72154779 None:intergenic
CAAGTTCTTTCCAAAATGCT+TGG 0.406902 8.3:-72156388 None:intergenic
TCTAATCCATCTGCAGCATA+AGG 0.407144 8.3:-72156278 None:intergenic
AAGCTATCTGCCTGTGGATG+TGG 0.409669 8.3:-72155495 None:intergenic
TATAATGGTCAAGTCAATTA+GGG 0.409856 8.3:-72156684 None:intergenic
GGCAAGTAAATATCACTAAC+AGG 0.412329 8.3:-72155522 None:intergenic
GAAGGATAAAGCAAAGTATA+CGG 0.412453 8.3:-72156899 None:intergenic
GAGACTCTAAGGAATCGATA+TGG 0.416853 8.3:+72156863 MS.gene07830:CDS
GTCAATCCTTCCTCACTTGA+AGG 0.416919 8.3:-72156917 None:intergenic
AATCTCTTGAAAGCCTCTAA+TGG 0.418384 8.3:-72156788 None:intergenic
CTATTTGTTGCTATGATGAA+TGG 0.420998 8.3:-72155745 None:intergenic
CTATCTAGCTTGCTCTTTGG+TGG 0.425209 8.3:-72155116 None:intergenic
CCACCACCTAAAATCATTCA+AGG 0.428002 8.3:+72154790 MS.gene07830:CDS
GATAGTGACATGGGAATTCC+AGG 0.428040 8.3:+72153710 MS.gene07830:CDS
CACCAAAGAGCAAGCTAGAT+AGG 0.433954 8.3:+72155117 MS.gene07830:CDS
TCAACTTTGTTTGCAACTCA+TGG 0.435514 8.3:+72153816 MS.gene07830:CDS
AAGTTGATGGTTCCATGGTT+AGG 0.438897 8.3:-72153800 None:intergenic
AGAAAGTGGAAAAGGTAAAG+TGG 0.439449 8.3:+72153601 MS.gene07830:intron
TAGCCCTATACCATTGTTCA+AGG 0.440814 8.3:+72154729 MS.gene07830:CDS
AGTTTATAACCTTTCTTAGC+TGG 0.442387 8.3:-72154206 None:intergenic
GTTCCTTGAACAATGGTATA+GGG 0.442713 8.3:-72154732 None:intergenic
TCTTGACAAGACCTCTATAA+TGG 0.447317 8.3:-72156699 None:intergenic
TGGGCCGTTCAGTGGCTCTC+AGG 0.448781 8.3:+72152932 MS.gene07830:CDS
TATTGAGAAGACGTTCTTGA+TGG 0.451460 8.3:+72155876 MS.gene07830:CDS
TTCTCAATATAAAATGCTCC+TGG 0.455625 8.3:-72153728 None:intergenic
TTTACAAGAGAGATGATTGC+TGG 0.455862 8.3:+72154932 MS.gene07830:CDS
AAATATCACAGGTGGATTCA+AGG 0.457407 8.3:+72152861 MS.gene07830:CDS
TTCAATTTGGCTCCTTGCCA+AGG 0.457965 8.3:+72155566 MS.gene07830:CDS
CTTAGAAAGCTCATCATACT+CGG 0.462104 8.3:-72156615 None:intergenic
CGGCCTTCGCCTTCTGATAG+AGG 0.462912 8.3:+72156250 MS.gene07830:CDS
GGAAGTAAAAGCTTATGAAT+AGG 0.477117 8.3:-72155787 None:intergenic
GGACAGGTAAACGTGAAGAA+TGG 0.481824 8.3:+72154054 MS.gene07830:CDS
CTGAAGACTTTACGAGGAGA+TGG 0.485246 8.3:+72154032 MS.gene07830:CDS
TGGAAAGAACTTGTAGAGGT+TGG 0.486789 8.3:+72156398 MS.gene07830:CDS
CATCCACAGGCAGATAGCTT+TGG 0.493258 8.3:+72155498 MS.gene07830:CDS
CACTGTCCAGCTCCTAGAAT+CGG 0.493765 8.3:-72153662 None:intergenic
AATAGAGCTGGTGTTTCACT+TGG 0.494388 8.3:-72153987 None:intergenic
GTGTTGGATCCTCCAATAAC+GGG 0.494877 8.3:-72154146 None:intergenic
CGAAGGCCGTGTGGGGAAGT+AGG 0.495559 8.3:-72156236 None:intergenic
TTAGCTCGTCCCGTTATTGG+AGG 0.499661 8.3:+72154137 MS.gene07830:CDS
TTCAATCTTGGGCCGTTCAG+TGG 0.504725 8.3:+72152924 MS.gene07830:CDS
CTTTATCCTTCAAGTGAGGA+AGG 0.508215 8.3:+72156911 MS.gene07830:CDS
TGCCTAATGATCTACTCAAA+AGG 0.508255 8.3:+72155962 MS.gene07830:CDS
GACAGGTAAACGTGAAGAAT+GGG 0.514304 8.3:+72154055 MS.gene07830:CDS
GATATCAACGCCCTCACCGC+TGG 0.515402 8.3:+72152835 MS.gene07830:CDS
CTAATCCATCTGCAGCATAA+GGG 0.518394 8.3:-72156277 None:intergenic
TGATGGAACTTTGAAGCCGT+TGG 0.518852 8.3:+72155455 MS.gene07830:CDS
TACCTCGGACAGAGAATTGA+AGG 0.519761 8.3:+72156740 MS.gene07830:CDS
TCCATTTCACAGAAGCCAAA+AGG 0.520125 8.3:+72152774 MS.gene07830:CDS
GGTGTTGGATCCTCCAATAA+CGG 0.521313 8.3:-72154147 None:intergenic
AAACAAAGTTGATGGTTCCA+TGG 0.521970 8.3:-72153805 None:intergenic
AATTGACTTGACCATTATAG+AGG 0.522463 8.3:+72156688 MS.gene07830:CDS
TATGGGCTGTTATTAAGTCA+TGG 0.522967 8.3:+72156303 MS.gene07830:CDS
AGAAGATTAATGCCTAAGAA+AGG 0.525519 8.3:+72156587 MS.gene07830:CDS
GATATTTAAACCAGCGGTGA+GGG 0.525980 8.3:-72152845 None:intergenic
TATATTGAGAATTTCATGCT+AGG 0.525987 8.3:+72153740 MS.gene07830:CDS
ATATTTGTAGAAAGTGGAAA+AGG 0.528541 8.3:+72153593 MS.gene07830:intron
GTGCTGAAGCAATCCATATG+AGG 0.530017 8.3:-72156496 None:intergenic
TCCGCACCGATTCTAGGAGC+TGG 0.530615 8.3:+72153656 MS.gene07830:CDS
AGCTATCTGCCTGTGGATGT+GGG 0.530871 8.3:-72155494 None:intergenic
TGCACTTGCAAGGAGTGTCT+TGG 0.531139 8.3:+72155837 MS.gene07830:CDS
ATGGGCTGTTATTAAGTCAT+GGG 0.531811 8.3:+72156304 MS.gene07830:CDS
CTGAGGAGTTCCTTGAACAA+TGG 0.531953 8.3:-72154739 None:intergenic
GGAAAGAGATCGTTTCTTGA+AGG 0.532072 8.3:+72153623 MS.gene07830:CDS
AATAAATGGAGCTGATACAA+AGG 0.534187 8.3:+72155398 MS.gene07830:CDS
TGCAGATGGATTAGAGATAT+GGG 0.538537 8.3:+72156286 MS.gene07830:CDS
TCACAGAAGCCAAAAGGTGA+AGG 0.539609 8.3:+72152780 MS.gene07830:CDS
TTTCTTAGCTGGTTTCCTGC+CGG 0.541247 8.3:-72154195 None:intergenic
GGACAGTGATATTGATGCAT+AGG 0.541977 8.3:+72152803 MS.gene07830:CDS
GAAAGTGGAAAAGGTAAAGT+GGG 0.542323 8.3:+72153602 MS.gene07830:intron
GTAACTGCAGATGTTAATGT+AGG 0.545905 8.3:-72154610 None:intergenic
TCATCAAAGGTGTTAAACTC+TGG 0.547395 8.3:-72154649 None:intergenic
TTAGAAAGCTCATCATACTC+GGG 0.548951 8.3:-72156614 None:intergenic
GCTTTCTAAGAACTATCAAA+AGG 0.551115 8.3:+72156628 MS.gene07830:CDS
GACTACCCTTATGCTGCAGA+TGG 0.551288 8.3:+72156272 MS.gene07830:CDS
AGCTCTATATTTGACACCTA+CGG 0.553269 8.3:+72152901 MS.gene07830:CDS
GGCAGGAAACCAGCTAAGAA+AGG 0.554760 8.3:+72154197 MS.gene07830:CDS
CACAGAAGCCAAAAGGTGAA+GGG 0.557115 8.3:+72152781 MS.gene07830:CDS
AGCTTCAACAAAACATGGGT+GGG 0.558582 8.3:+72155180 MS.gene07830:CDS
TTTCTTGTTATCTAGAGGTG+TGG 0.558961 8.3:+72156202 MS.gene07830:intron
GCTAAGTAGCCCACATCCAC+AGG 0.561034 8.3:+72155485 MS.gene07830:CDS
TATACCAATGGCTGATGAGT+TGG 0.561542 8.3:-72155620 None:intergenic
AAGGAACTCCTCAGAACTGA+TGG 0.562150 8.3:+72154748 MS.gene07830:CDS
GTTTACAACTACATAAGCCT+TGG 0.564708 8.3:-72155583 None:intergenic
AGAACAGCTTCAACAAAACA+TGG 0.564781 8.3:+72155175 MS.gene07830:CDS
ACCGCTGGTTTAAATATCAC+AGG 0.565086 8.3:+72152850 MS.gene07830:CDS
CACCTTGCCTTACCCTCGAA+GGG 0.565890 8.3:+72154166 MS.gene07830:CDS
TCAGAAGGCGAAGGCCGTGT+GGG 0.568063 8.3:-72156244 None:intergenic
ATTGAGAAGACGTTCTTGAT+GGG 0.569095 8.3:+72155877 MS.gene07830:CDS
ATCACCAACTCATCAGCCAT+TGG 0.576161 8.3:+72155616 MS.gene07830:CDS
TGAAGACTTTACGAGGAGAT+GGG 0.579763 8.3:+72154033 MS.gene07830:CDS
AAATGCACGCACATCATCAA+AGG 0.579942 8.3:-72154662 None:intergenic
ATGTGGGCTACTTAGCTCAA+TGG 0.583626 8.3:-72155478 None:intergenic
CTATAGTAGACTAATAGAGC+TGG 0.586525 8.3:-72153999 None:intergenic
GAAAAGTGAATCAATCTGGA+TGG 0.587432 8.3:+72154894 MS.gene07830:intron
ATTGATGCATAGGAATGTGT+TGG 0.588062 8.3:+72152813 MS.gene07830:CDS
ACACCTTGCCTTACCCTCGA+AGG 0.589170 8.3:+72154165 MS.gene07830:CDS
AAGCTAGATAGGCAACTCTA+CGG 0.589256 8.3:+72155128 MS.gene07830:CDS
ATTTACTTGCCTGCAAGTGA+TGG 0.592859 8.3:+72155534 MS.gene07830:CDS
TCTAAGGAATCGATATGGAC+CGG 0.593095 8.3:+72156868 MS.gene07830:CDS
TCGAGGGTAAGGCAAGGTGT+TGG 0.593510 8.3:-72154162 None:intergenic
TTAGGTGGTGGAAACTTAAG+GGG 0.594767 8.3:-72154778 None:intergenic
CTTGAAGATATTCCTAACCA+TGG 0.597036 8.3:+72153788 MS.gene07830:CDS
ATCAGAAGGCGAAGGCCGTG+TGG 0.599096 8.3:-72156245 None:intergenic
GAGGAAGGATTGACTTGCAG+AGG 0.599561 8.3:+72156926 MS.gene07830:CDS
TAGAAAGCTCATCATACTCG+GGG 0.602660 8.3:-72156613 None:intergenic
CACCTTCAATTCTCTGTCCG+AGG 0.602919 8.3:-72156742 None:intergenic
CAACTACATAAGCCTTGGCA+AGG 0.604594 8.3:-72155578 None:intergenic
AGCTCCTAGAATCGGTGCGG+AGG 0.607434 8.3:-72153654 None:intergenic
GATATGGATATATTGAGTCA+TGG 0.608683 8.3:-72155368 None:intergenic
TTTCAATGGGATAGTGACAT+GGG 0.609030 8.3:+72153701 MS.gene07830:CDS
ACTTTACGAGGAGATGGGAC+AGG 0.611272 8.3:+72154038 MS.gene07830:CDS
AACAAATAGGCATCTTAGTG+TGG 0.616014 8.3:+72155759 MS.gene07830:CDS
TTCATTGTGTCACGATAGTG+CGG 0.616157 8.3:-72155808 None:intergenic
ATTAGTGCTACTAGTGCTGA+TGG 0.617007 8.3:+72152958 MS.gene07830:CDS
AAGGGTAGTCCTCTATCAGA+AGG 0.619758 8.3:-72156259 None:intergenic
ACAGAAGCCAAAAGGTGAAG+GGG 0.621182 8.3:+72152782 MS.gene07830:CDS
CAGCTTCAACAAAACATGGG+TGG 0.621253 8.3:+72155179 MS.gene07830:CDS
TGCTGAAGCAATCCATATGA+GGG 0.621259 8.3:-72156495 None:intergenic
ACTCAATATATCCATATCTG+AGG 0.622340 8.3:+72155373 MS.gene07830:CDS
ACCTGTGATATTTAAACCAG+CGG 0.625148 8.3:-72152851 None:intergenic
CAGAAGGCGAAGGCCGTGTG+GGG 0.628083 8.3:-72156243 None:intergenic
GAGTGTCTTGGTAAATGCAG+AGG 0.628961 8.3:+72155849 MS.gene07830:CDS
GTGCTTCACCATCAGTTCTG+AGG 0.630572 8.3:-72154756 None:intergenic
ACGGCCCAAGATTGAACCGT+AGG 0.632707 8.3:-72152917 None:intergenic
TGATATTTAAACCAGCGGTG+AGG 0.636637 8.3:-72152846 None:intergenic
TGAACATTAATGCACTTGCA+AGG 0.638089 8.3:+72155827 MS.gene07830:CDS
CAGATCCTAAAAGTGAGAGA+CGG 0.638615 8.3:+72154488 MS.gene07830:intron
AGTGTCTTGGTAAATGCAGA+GGG 0.642136 8.3:+72155850 MS.gene07830:CDS
GTAAACGTGAAGAATGGGAA+AGG 0.646192 8.3:+72154060 MS.gene07830:CDS
TTTGCTTTATCCTTCAAGTG+AGG 0.646310 8.3:+72156907 MS.gene07830:CDS
CTATGATGTCTACAATGATG+TGG 0.649661 8.3:+72154091 MS.gene07830:CDS
GCTGGTTTAAATATCACAGG+TGG 0.653722 8.3:+72152853 MS.gene07830:CDS
TTAAGAATGTAACCTCCGTA+CGG 0.656295 8.3:-72156548 None:intergenic
CTACTTAGCTCAATGGCCAA+CGG 0.658352 8.3:-72155471 None:intergenic
ACCTTGCCTTACCCTCGAAG+GGG 0.659200 8.3:+72154167 MS.gene07830:CDS
GCTTCTGATGAACAATACCT+CGG 0.660873 8.3:+72156725 MS.gene07830:CDS
AATGGCTGATGAGTTGGTGA+TGG 0.664841 8.3:-72155614 None:intergenic
TCAACCTGAGAGCCACTGAA+CGG 0.665209 8.3:-72152936 None:intergenic
TTTGGACAATATCCGTACGG+AGG 0.665441 8.3:+72156536 MS.gene07830:CDS
GAACTTGTAGAGGTTGGTCA+CGG 0.669529 8.3:+72156404 MS.gene07830:CDS
GGACCAAAGCTATCTGCCTG+TGG 0.675031 8.3:-72155501 None:intergenic
AGTCCTCTATCAGAAGGCGA+AGG 0.676314 8.3:-72156253 None:intergenic
TATGATGTCTACAATGATGT+GGG 0.681464 8.3:+72154092 MS.gene07830:CDS
CCTTGCCTTACCCTCGAAGG+GGG 0.681885 8.3:+72154168 MS.gene07830:CDS
TCCAGCTCCTAGAATCGGTG+CGG 0.683704 8.3:-72153657 None:intergenic
GAACAGCTTCAACAAAACAT+GGG 0.695205 8.3:+72155176 MS.gene07830:CDS
AAGGATCCTACTTCCCCACA+CGG 0.713360 8.3:+72156230 MS.gene07830:CDS
GAAGAACTGAAGACTTTACG+AGG 0.717242 8.3:+72154026 MS.gene07830:CDS
AGAAAGCTCATCATACTCGG+GGG 0.733263 8.3:-72156612 None:intergenic
TGAAGCAATCCATATGAGGG+TGG 0.741108 8.3:-72156492 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTATATATTTTTTAAT+AGG + chr8.3:72155311-72155330 MS.gene07830:intron 0.0%
!! TTTGATATCAATATTATAAA+CGG - chr8.3:72156151-72156170 None:intergenic 10.0%
!! AAATATATCAAGCAATAATA+AGG - chr8.3:72155083-72155102 None:intergenic 15.0%
!!! CAATATTCTTATTTTAGTTT+TGG - chr8.3:72155026-72155045 None:intergenic 15.0%
!!! TAATGGAATATTTCTATTTT+AGG - chr8.3:72153113-72153132 None:intergenic 15.0%
!! AGGTTAACAATTAAAAAACT+AGG - chr8.3:72155063-72155082 None:intergenic 20.0%
!! ATTTGTATCAAAAAAGAAAG+AGG - chr8.3:72153282-72153301 None:intergenic 20.0%
!! TAAAAAACTAGGATGAAAAA+TGG - chr8.3:72155052-72155071 None:intergenic 20.0%
!! TATATTCAGTAGTTAAAAAG+AGG + chr8.3:72156014-72156033 MS.gene07830:intron 20.0%
!! TTGCTTGATATATTTAATGT+AGG + chr8.3:72155088-72155107 MS.gene07830:intron 20.0%
!!! AGTTAGTATAGTATGTTTTA+AGG + chr8.3:72154252-72154271 MS.gene07830:intron 20.0%
!!! ATACAAAGTTCATTTTCAAT+GGG + chr8.3:72153688-72153707 MS.gene07830:CDS 20.0%
!!! ATTTTTAGTCATGCAAAATT+TGG + chr8.3:72154829-72154848 MS.gene07830:intron 20.0%
!!! ATTTTTCATAATTACCTTGT+TGG - chr8.3:72153885-72153904 None:intergenic 20.0%
!!! TACTTAATAGTGTCAAATTT+TGG - chr8.3:72153945-72153964 None:intergenic 20.0%
!!! TCATATTTTAAACTCACAAT+TGG + chr8.3:72153343-72153362 MS.gene07830:intron 20.0%
!!! TTATGATGGCATTATTTTTT+TGG + chr8.3:72154367-72154386 MS.gene07830:intron 20.0%
!!! TTTTAATGTCTAAACATCAA+TGG + chr8.3:72156083-72156102 MS.gene07830:intron 20.0%
! ATATTTGTAGAAAGTGGAAA+AGG + chr8.3:72153593-72153612 MS.gene07830:intron 25.0%
! ATCAAGTATGTACAATTTGT+TGG - chr8.3:72155346-72155365 None:intergenic 25.0%
! ATTGTCTGCTGTATTATATA+AGG + chr8.3:72155915-72155934 MS.gene07830:CDS 25.0%
! GTCAATTGAATTTATACCAA+TGG - chr8.3:72155635-72155654 None:intergenic 25.0%
! GTCACACATATAGATATTAA+CGG - chr8.3:72155698-72155717 None:intergenic 25.0%
! GTTGAATGCACAAATATTAA+TGG - chr8.3:72153130-72153149 None:intergenic 25.0%
! TAACTACTGAATATATGTCT+AGG - chr8.3:72156009-72156028 None:intergenic 25.0%
! TATAATGGTCAAGTCAATTA+GGG - chr8.3:72156687-72156706 None:intergenic 25.0%
! TATATTGAGAATTTCATGCT+AGG + chr8.3:72153740-72153759 MS.gene07830:CDS 25.0%
! TCACATTATCAAAAGAATTC+AGG + chr8.3:72154964-72154983 MS.gene07830:CDS 25.0%
! TGCTGTATTATATAAGGATT+GGG + chr8.3:72155921-72155940 MS.gene07830:CDS 25.0%
! TTAATATCTATATGTGTGAC+AGG + chr8.3:72155698-72155717 MS.gene07830:intron 25.0%
!! AAACGGAAAGTTTAAAATTG+CGG - chr8.3:72156134-72156153 None:intergenic 25.0%
!! ATCATTTTGTAAGAAAAGGA+AGG - chr8.3:72155439-72155458 None:intergenic 25.0%
!! CATACAAAGTTCATTTTCAA+TGG + chr8.3:72153687-72153706 MS.gene07830:CDS 25.0%
!! GACTAAAAATGTGTTTCTAA+TGG - chr8.3:72154819-72154838 None:intergenic 25.0%
!! TTCCATCATTTTGTAAGAAA+AGG - chr8.3:72155443-72155462 None:intergenic 25.0%
!! TTCCTTTTCTTACAAAATGA+TGG + chr8.3:72155438-72155457 MS.gene07830:CDS 25.0%
!!! ATTAATCTTCTGCTTTTAGT+TGG - chr8.3:72156578-72156597 None:intergenic 25.0%
!!! TGAATTCTTTTGATAATGTG+AGG - chr8.3:72154965-72154984 None:intergenic 25.0%
!!! TTTTGAAAAGTGAATCAATC+TGG + chr8.3:72154890-72154909 MS.gene07830:intron 25.0%
AATAAATGGAGCTGATACAA+AGG + chr8.3:72155398-72155417 MS.gene07830:CDS 30.0%
ACTCAATATATCCATATCTG+AGG + chr8.3:72155373-72155392 MS.gene07830:CDS 30.0%
AGAAGATTAATGCCTAAGAA+AGG + chr8.3:72156587-72156606 MS.gene07830:CDS 30.0%
AGTTTATAACCTTTCTTAGC+TGG - chr8.3:72154209-72154228 None:intergenic 30.0%
ATCATAACATACCCTATACT+AGG - chr8.3:72154354-72154373 None:intergenic 30.0%
CATTCATCATAGCAACAAAT+AGG + chr8.3:72155746-72155765 MS.gene07830:CDS 30.0%
CCATATCTGAGGAAAATAAA+TGG + chr8.3:72155384-72155403 MS.gene07830:CDS 30.0%
CTATAATGGTCAAGTCAATT+AGG - chr8.3:72156688-72156707 None:intergenic 30.0%
CTGCTGTATTATATAAGGAT+TGG + chr8.3:72155920-72155939 MS.gene07830:CDS 30.0%
GAACGTCAAATATAAACATG+TGG + chr8.3:72153380-72153399 MS.gene07830:intron 30.0%
GAAGGATAAAGCAAAGTATA+CGG - chr8.3:72156902-72156921 None:intergenic 30.0%
GATATGGATATATTGAGTCA+TGG - chr8.3:72155371-72155390 None:intergenic 30.0%
GGAAGTAAAAGCTTATGAAT+AGG - chr8.3:72155790-72155809 None:intergenic 30.0%
TAGTATAGGGTATGTTATGA+TGG + chr8.3:72154353-72154372 MS.gene07830:intron 30.0%
TATGATGTCTACAATGATGT+GGG + chr8.3:72154092-72154111 MS.gene07830:CDS 30.0%
TCATAACATACCCTATACTA+GGG - chr8.3:72154353-72154372 None:intergenic 30.0%
TGTGACATATTTGTAGAAAG+TGG + chr8.3:72153587-72153606 MS.gene07830:intron 30.0%
TTCTCAATATAAAATGCTCC+TGG - chr8.3:72153731-72153750 None:intergenic 30.0%
TTGAACAAAAACTCACTCAA+AGG + chr8.3:72156831-72156850 MS.gene07830:CDS 30.0%
! AATGTTTTCATCCCTAGTAT+AGG + chr8.3:72154339-72154358 MS.gene07830:intron 30.0%
! AATTTTGGACAATATCCGTA+CGG + chr8.3:72156533-72156552 MS.gene07830:CDS 30.0%
! ATACCTTGAATGATTTTAGG+TGG - chr8.3:72154796-72154815 None:intergenic 30.0%
! ATGTTTTCATCCCTAGTATA+GGG + chr8.3:72154340-72154359 MS.gene07830:intron 30.0%
! CTAGGATCTAATGTTTTGTA+AGG - chr8.3:72155991-72156010 None:intergenic 30.0%
! CTATTTGTTGCTATGATGAA+TGG - chr8.3:72155748-72155767 None:intergenic 30.0%
! GCTTTCTAAGAACTATCAAA+AGG + chr8.3:72156628-72156647 MS.gene07830:CDS 30.0%
! TGGATACCTTGAATGATTTT+AGG - chr8.3:72154799-72154818 None:intergenic 30.0%
! TTTAAGGTATTTCTGCACAA+AGG + chr8.3:72154268-72154287 MS.gene07830:intron 30.0%
!! AATTGACTTGACCATTATAG+AGG + chr8.3:72156688-72156707 MS.gene07830:CDS 30.0%
!! ATTTTGGAAAGAACTTGTAG+AGG + chr8.3:72156394-72156413 MS.gene07830:CDS 30.0%
!! CCATTTATTTTCCTCAGATA+TGG - chr8.3:72155387-72155406 None:intergenic 30.0%
!! TACCTTTTGAGTAGATCATT+AGG - chr8.3:72155967-72155986 None:intergenic 30.0%
!! TATTGTTAACTTGTGCTCTA+AGG + chr8.3:72153073-72153092 MS.gene07830:intron 30.0%
!!! TCAATTAGGGTGTCATTTTT+TGG - chr8.3:72156674-72156693 None:intergenic 30.0%
AAAGTATACGGCATCTTTAC+CGG - chr8.3:72156890-72156909 None:intergenic 35.0%
AACAAATAGGCATCTTAGTG+TGG + chr8.3:72155759-72155778 MS.gene07830:CDS 35.0%
AACAATGAGTTACGAGTATC+CGG - chr8.3:72153253-72153272 None:intergenic 35.0%
AATCTCTTGAAAGCCTCTAA+TGG - chr8.3:72156791-72156810 None:intergenic 35.0%
ACACCTCTAGATAACAAGAA+AGG - chr8.3:72156203-72156222 None:intergenic 35.0%
ACCTGTGATATTTAAACCAG+CGG - chr8.3:72152854-72152873 None:intergenic 35.0%
ACTCATTAATCTGTGACTTG+AGG + chr8.3:72153211-72153230 MS.gene07830:intron 35.0%
ACTTGAAAAATGCAACATGG+TGG + chr8.3:72156429-72156448 MS.gene07830:CDS 35.0%
AGAAAGTGGAAAAGGTAAAG+TGG + chr8.3:72153601-72153620 MS.gene07830:intron 35.0%
AGAACAGCTTCAACAAAACA+TGG + chr8.3:72155175-72155194 MS.gene07830:CDS 35.0%
AGCTCTATATTTGACACCTA+CGG + chr8.3:72152901-72152920 MS.gene07830:CDS 35.0%
AGTTCCTTGAACAATGGTAT+AGG - chr8.3:72154736-72154755 None:intergenic 35.0%
ATTGAGAAGACGTTCTTGAT+GGG + chr8.3:72155877-72155896 MS.gene07830:CDS 35.0%
ATTGATGCATAGGAATGTGT+TGG + chr8.3:72152813-72152832 MS.gene07830:CDS 35.0%
CAAACATAATGTTCACCACA+TGG + chr8.3:72153034-72153053 MS.gene07830:intron 35.0%
CAAGTTCTTTCCAAAATGCT+TGG - chr8.3:72156391-72156410 None:intergenic 35.0%
CACATGGATGTATACTTAAC+GGG + chr8.3:72153050-72153069 MS.gene07830:intron 35.0%
CCACATGGATGTATACTTAA+CGG + chr8.3:72153049-72153068 MS.gene07830:intron 35.0%
CTATAGTAGACTAATAGAGC+TGG - chr8.3:72154002-72154021 None:intergenic 35.0%
CTATGATGTCTACAATGATG+TGG + chr8.3:72154091-72154110 MS.gene07830:CDS 35.0%
CTCATTAATCTGTGACTTGA+GGG + chr8.3:72153212-72153231 MS.gene07830:intron 35.0%
CTTAGAAAGCTCATCATACT+CGG - chr8.3:72156618-72156637 None:intergenic 35.0%
CTTCCAAGAGATGAATCATT+TGG + chr8.3:72154526-72154545 MS.gene07830:CDS 35.0%
CTTGAAAAATGCAACATGGT+GGG + chr8.3:72156430-72156449 MS.gene07830:CDS 35.0%
CTTGAAGATATTCCTAACCA+TGG + chr8.3:72153788-72153807 MS.gene07830:CDS 35.0%
GAAAAGTGAATCAATCTGGA+TGG + chr8.3:72154894-72154913 MS.gene07830:intron 35.0%
GAAAGTGGAAAAGGTAAAGT+GGG + chr8.3:72153602-72153621 MS.gene07830:intron 35.0%
GAACAGCTTCAACAAAACAT+GGG + chr8.3:72155176-72155195 MS.gene07830:CDS 35.0%
GAATATCCCATCTTAGATTG+AGG - chr8.3:72156059-72156078 None:intergenic 35.0%
GGCAAGTAAATATCACTAAC+AGG - chr8.3:72155525-72155544 None:intergenic 35.0%
GTAACTGCAGATGTTAATGT+AGG - chr8.3:72154613-72154632 None:intergenic 35.0%
GTGACTTGAAAAATGCAACA+TGG + chr8.3:72156426-72156445 MS.gene07830:CDS 35.0%
GTTCCTTGAACAATGGTATA+GGG - chr8.3:72154735-72154754 None:intergenic 35.0%
GTTTACAACTACATAAGCCT+TGG - chr8.3:72155586-72155605 None:intergenic 35.0%
TATTGAGAAGACGTTCTTGA+TGG + chr8.3:72155876-72155895 MS.gene07830:CDS 35.0%
TCTTGACAAGACCTCTATAA+TGG - chr8.3:72156702-72156721 None:intergenic 35.0%
TGAACATTAATGCACTTGCA+AGG + chr8.3:72155827-72155846 MS.gene07830:CDS 35.0%
TGACCAAATGATTCATCTCT+TGG - chr8.3:72154532-72154551 None:intergenic 35.0%
TGAGTTGCAAACAAAGTTGA+TGG - chr8.3:72153816-72153835 None:intergenic 35.0%
TGCCTAATGATCTACTCAAA+AGG + chr8.3:72155962-72155981 MS.gene07830:CDS 35.0%
TGTCTAAACATCAATGGTTG+TGG + chr8.3:72156089-72156108 MS.gene07830:intron 35.0%
TTAAGAATGTAACCTCCGTA+CGG - chr8.3:72156551-72156570 None:intergenic 35.0%
TTAGAAAGCTCATCATACTC+GGG - chr8.3:72156617-72156636 None:intergenic 35.0%
TTAGAGGCTTTCAAGAGATT+TGG + chr8.3:72156791-72156810 MS.gene07830:CDS 35.0%
TTCTGATTCACAACCATTAG+AGG + chr8.3:72156775-72156794 MS.gene07830:CDS 35.0%
TTTACAAGAGAGATGATTGC+TGG + chr8.3:72154932-72154951 MS.gene07830:CDS 35.0%
TTTCAATGGGATAGTGACAT+GGG + chr8.3:72153701-72153720 MS.gene07830:CDS 35.0%
TTTGCTTTATCCTTCAAGTG+AGG + chr8.3:72156907-72156926 MS.gene07830:CDS 35.0%
! AAATATCACAGGTGGATTCA+AGG + chr8.3:72152861-72152880 MS.gene07830:CDS 35.0%
! ATGGGCTGTTATTAAGTCAT+GGG + chr8.3:72156304-72156323 MS.gene07830:CDS 35.0%
! ATTCTGAACTCCAAGCATTT+TGG + chr8.3:72156378-72156397 MS.gene07830:CDS 35.0%
! CAAGAGATTTGGAACAAAGT+TGG + chr8.3:72156802-72156821 MS.gene07830:CDS 35.0%
! CAATCTAAGATGGGATATTC+AGG + chr8.3:72156059-72156078 MS.gene07830:intron 35.0%
! CTTCATGCAGCTGTTAATTT+TGG + chr8.3:72156518-72156537 MS.gene07830:CDS 35.0%
! CTTCCTTTCTTGTTATCTAG+AGG + chr8.3:72156197-72156216 MS.gene07830:intron 35.0%
! TATGGGCTGTTATTAAGTCA+TGG + chr8.3:72156303-72156322 MS.gene07830:CDS 35.0%
! TCAACTTTGTTTGCAACTCA+TGG + chr8.3:72153816-72153835 MS.gene07830:CDS 35.0%
! TTTCTTGTTATCTAGAGGTG+TGG + chr8.3:72156202-72156221 MS.gene07830:intron 35.0%
! TTTTCAATGGGATAGTGACA+TGG + chr8.3:72153700-72153719 MS.gene07830:CDS 35.0%
!! AAACAAAGTTGATGGTTCCA+TGG - chr8.3:72153808-72153827 None:intergenic 35.0%
!! ATCTTCTGCTTTTAGTTGGT+CGG - chr8.3:72156574-72156593 None:intergenic 35.0%
!! GTTTTGTTGAAGCTGTTCTT+TGG - chr8.3:72155174-72155193 None:intergenic 35.0%
!! TCATCAAAGGTGTTAAACTC+TGG - chr8.3:72154652-72154671 None:intergenic 35.0%
!! TGCAGATGGATTAGAGATAT+GGG + chr8.3:72156286-72156305 MS.gene07830:CDS 35.0%
!! TTTAGGTGGTGGAAACTTAA+GGG - chr8.3:72154782-72154801 None:intergenic 35.0%
!!! TTTTAGGTGGTGGAAACTTA+AGG - chr8.3:72154783-72154802 None:intergenic 35.0%
AAATGCACGCACATCATCAA+AGG - chr8.3:72154665-72154684 None:intergenic 40.0%
AAGCTAGATAGGCAACTCTA+CGG + chr8.3:72155128-72155147 MS.gene07830:CDS 40.0%
AATAGAGCTGGTGTTTCACT+TGG - chr8.3:72153990-72154009 None:intergenic 40.0%
AGCTTCAACAAAACATGGGT+GGG + chr8.3:72155180-72155199 MS.gene07830:CDS 40.0%
AGGGCACAAGTTAACATTTC+CGG + chr8.3:72153231-72153250 MS.gene07830:intron 40.0%
AGTGTCTTGGTAAATGCAGA+GGG + chr8.3:72155850-72155869 MS.gene07830:CDS 40.0%
ATTTACTTGCCTGCAAGTGA+TGG + chr8.3:72155534-72155553 MS.gene07830:CDS 40.0%
CAGATCCTAAAAGTGAGAGA+CGG + chr8.3:72154488-72154507 MS.gene07830:intron 40.0%
CCACCACCTAAAATCATTCA+AGG + chr8.3:72154790-72154809 MS.gene07830:CDS 40.0%
CCGTTAAGTATACATCCATG+TGG - chr8.3:72153052-72153071 None:intergenic 40.0%
CTAATCCATCTGCAGCATAA+GGG - chr8.3:72156280-72156299 None:intergenic 40.0%
CTGCACAAAGGAAAGTGATA+TGG + chr8.3:72154280-72154299 MS.gene07830:intron 40.0%
CTGTTCTTTGGTTATTGTGC+TGG - chr8.3:72155162-72155181 None:intergenic 40.0%
CTTTATCCTTCAAGTGAGGA+AGG + chr8.3:72156911-72156930 MS.gene07830:CDS 40.0%
GAAGAACTGAAGACTTTACG+AGG + chr8.3:72154026-72154045 MS.gene07830:CDS 40.0%
GACAGGTAAACGTGAAGAAT+GGG + chr8.3:72154055-72154074 MS.gene07830:CDS 40.0%
GATATTTAAACCAGCGGTGA+GGG - chr8.3:72152848-72152867 None:intergenic 40.0%
GCTTCTGATGAACAATACCT+CGG + chr8.3:72156725-72156744 MS.gene07830:CDS 40.0%
GGAAAGAGATCGTTTCTTGA+AGG + chr8.3:72153623-72153642 MS.gene07830:CDS 40.0%
GGAACAATGATGAGACTCTA+AGG + chr8.3:72156852-72156871 MS.gene07830:CDS 40.0%
GGACAGTGATATTGATGCAT+AGG + chr8.3:72152803-72152822 MS.gene07830:CDS 40.0%
GTAAACGTGAAGAATGGGAA+AGG + chr8.3:72154060-72154079 MS.gene07830:CDS 40.0%
TAGAAAGCTCATCATACTCG+GGG - chr8.3:72156616-72156635 None:intergenic 40.0%
TAGCCCTATACCATTGTTCA+AGG + chr8.3:72154729-72154748 MS.gene07830:CDS 40.0%
TATACCAATGGCTGATGAGT+TGG - chr8.3:72155623-72155642 None:intergenic 40.0%
TCCATTTCACAGAAGCCAAA+AGG + chr8.3:72152774-72152793 MS.gene07830:CDS 40.0%
TCTAAGGAATCGATATGGAC+CGG + chr8.3:72156868-72156887 MS.gene07830:CDS 40.0%
TCTAATCCATCTGCAGCATA+AGG - chr8.3:72156281-72156300 None:intergenic 40.0%
TGAAGACTTTACGAGGAGAT+GGG + chr8.3:72154033-72154052 MS.gene07830:CDS 40.0%
TGATATTTAAACCAGCGGTG+AGG - chr8.3:72152849-72152868 None:intergenic 40.0%
TGCTGAAGCAATCCATATGA+GGG - chr8.3:72156498-72156517 None:intergenic 40.0%
TGGAAAGAACTTGTAGAGGT+TGG + chr8.3:72156398-72156417 MS.gene07830:CDS 40.0%
TTCATTGTGTCACGATAGTG+CGG - chr8.3:72155811-72155830 None:intergenic 40.0%
TTCGCAACCTCAATCTAAGA+TGG + chr8.3:72156049-72156068 MS.gene07830:intron 40.0%
! ACGCATTTTCTTTGCCAACA+AGG + chr8.3:72153868-72153887 MS.gene07830:CDS 40.0%
! ATGGCGTTGAAGCTTCAATT+TGG + chr8.3:72155553-72155572 MS.gene07830:CDS 40.0%
! ATTAGTGCTACTAGTGCTGA+TGG + chr8.3:72152958-72152977 MS.gene07830:CDS 40.0%
! GAGACTCTAAGGAATCGATA+TGG + chr8.3:72156863-72156882 MS.gene07830:CDS 40.0%
! GCTGGTTTAAATATCACAGG+TGG + chr8.3:72152853-72152872 MS.gene07830:CDS 40.0%
! TCGCAACCTCAATCTAAGAT+GGG + chr8.3:72156050-72156069 MS.gene07830:intron 40.0%
!! AAGTTGATGGTTCCATGGTT+AGG - chr8.3:72153803-72153822 None:intergenic 40.0%
!! ACCGCTGGTTTAAATATCAC+AGG + chr8.3:72152850-72152869 MS.gene07830:CDS 40.0%
!! CCTTGAATGATTTTAGGTGG+TGG - chr8.3:72154793-72154812 None:intergenic 40.0%
!! CTGCAGATGGATTAGAGATA+TGG + chr8.3:72156285-72156304 MS.gene07830:CDS 40.0%
!! TTAGGTGGTGGAAACTTAAG+GGG - chr8.3:72154781-72154800 None:intergenic 40.0%
!!! ACCTTTTGGCTTCTGTGAAA+TGG - chr8.3:72152778-72152797 None:intergenic 40.0%
!!! GCTAAGCTAGCTTTTCTATC+AGG - chr8.3:72154122-72154141 None:intergenic 40.0%
AAGGAACTCCTCAGAACTGA+TGG + chr8.3:72154748-72154767 MS.gene07830:CDS 45.0%
AAGGGTAGTCCTCTATCAGA+AGG - chr8.3:72156262-72156281 None:intergenic 45.0%
ACAGAAGCCAAAAGGTGAAG+GGG + chr8.3:72152782-72152801 MS.gene07830:CDS 45.0%
AGAAAGCTCATCATACTCGG+GGG - chr8.3:72156615-72156634 None:intergenic 45.0%
ATCACCAACTCATCAGCCAT+TGG + chr8.3:72155616-72155635 MS.gene07830:CDS 45.0%
CAACTACATAAGCCTTGGCA+AGG - chr8.3:72155581-72155600 None:intergenic 45.0%
CACAGAAGCCAAAAGGTGAA+GGG + chr8.3:72152781-72152800 MS.gene07830:CDS 45.0%
CACCAAAGAGCAAGCTAGAT+AGG + chr8.3:72155117-72155136 MS.gene07830:CDS 45.0%
CAGCTTCAACAAAACATGGG+TGG + chr8.3:72155179-72155198 MS.gene07830:CDS 45.0%
CTACTTAGCTCAATGGCCAA+CGG - chr8.3:72155474-72155493 None:intergenic 45.0%
CTGAAGACTTTACGAGGAGA+TGG + chr8.3:72154032-72154051 MS.gene07830:CDS 45.0%
CTGAGGAGTTCCTTGAACAA+TGG - chr8.3:72154742-72154761 None:intergenic 45.0%
GACACCTACGGTTCAATCTT+GGG + chr8.3:72152913-72152932 MS.gene07830:CDS 45.0%
GAGTGTCTTGGTAAATGCAG+AGG + chr8.3:72155849-72155868 MS.gene07830:CDS 45.0%
GATAGTGACATGGGAATTCC+AGG + chr8.3:72153710-72153729 MS.gene07830:CDS 45.0%
GGACAGGTAAACGTGAAGAA+TGG + chr8.3:72154054-72154073 MS.gene07830:CDS 45.0%
GTCAATCCTTCCTCACTTGA+AGG - chr8.3:72156920-72156939 None:intergenic 45.0%
GTGCTGAAGCAATCCATATG+AGG - chr8.3:72156499-72156518 None:intergenic 45.0%
GTGTTGGATCCTCCAATAAC+GGG - chr8.3:72154149-72154168 None:intergenic 45.0%
TCACAGAAGCCAAAAGGTGA+AGG + chr8.3:72152780-72152799 MS.gene07830:CDS 45.0%
TGAAGCAATCCATATGAGGG+TGG - chr8.3:72156495-72156514 None:intergenic 45.0%
TGACACCTACGGTTCAATCT+TGG + chr8.3:72152912-72152931 MS.gene07830:CDS 45.0%
TTTGGACAATATCCGTACGG+AGG + chr8.3:72156536-72156555 MS.gene07830:CDS 45.0%
! ACAGGTGGATTCAAGGTTCT+TGG + chr8.3:72152868-72152887 MS.gene07830:CDS 45.0%
! ATCTAGAGGTGTGGCTCTTA+AGG + chr8.3:72156211-72156230 MS.gene07830:intron 45.0%
! ATGTGGGCTACTTAGCTCAA+TGG - chr8.3:72155481-72155500 None:intergenic 45.0%
! GAACTTGTAGAGGTTGGTCA+CGG + chr8.3:72156404-72156423 MS.gene07830:CDS 45.0%
! GATGTGCGTGCATTTTACGA+GGG + chr8.3:72154670-72154689 MS.gene07830:CDS 45.0%
! GATTGGGTTTTCACTGAGCA+AGG + chr8.3:72155937-72155956 MS.gene07830:CDS 45.0%
! GGTGTTGGATCCTCCAATAA+CGG - chr8.3:72154150-72154169 None:intergenic 45.0%
! TGATGTGCGTGCATTTTACG+AGG + chr8.3:72154669-72154688 MS.gene07830:CDS 45.0%
! TTCAATTTGGCTCCTTGCCA+AGG + chr8.3:72155566-72155585 MS.gene07830:CDS 45.0%
! TTTCTTAGCTGGTTTCCTGC+CGG - chr8.3:72154198-72154217 None:intergenic 45.0%
!! AATGGCTGATGAGTTGGTGA+TGG - chr8.3:72155617-72155636 None:intergenic 45.0%
!! CTATCTAGCTTGCTCTTTGG+TGG - chr8.3:72155119-72155138 None:intergenic 45.0%
!! TGATGGAACTTTGAAGCCGT+TGG + chr8.3:72155455-72155474 MS.gene07830:CDS 45.0%
!! TGCCTATCTAGCTTGCTCTT+TGG - chr8.3:72155122-72155141 None:intergenic 45.0%
AAGCTATCTGCCTGTGGATG+TGG - chr8.3:72155498-72155517 None:intergenic 50.0%
AAGGATCCTACTTCCCCACA+CGG + chr8.3:72156230-72156249 MS.gene07830:CDS 50.0%
ACACTTGCACCACCCTCATA+TGG + chr8.3:72156483-72156502 MS.gene07830:CDS 50.0%
ACTTTACGAGGAGATGGGAC+AGG + chr8.3:72154038-72154057 MS.gene07830:CDS 50.0%
AGCTATCTGCCTGTGGATGT+GGG - chr8.3:72155497-72155516 None:intergenic 50.0%
AGCTTAGCTCGTCCCGTTAT+TGG + chr8.3:72154134-72154153 MS.gene07830:CDS 50.0%
AGTCCTCTATCAGAAGGCGA+AGG - chr8.3:72156256-72156275 None:intergenic 50.0%
CACTGTCCAGCTCCTAGAAT+CGG - chr8.3:72153665-72153684 None:intergenic 50.0%
CATCCACAGGCAGATAGCTT+TGG + chr8.3:72155498-72155517 MS.gene07830:CDS 50.0%
GACTACCCTTATGCTGCAGA+TGG + chr8.3:72156272-72156291 MS.gene07830:CDS 50.0%
GGCAGGAAACCAGCTAAGAA+AGG + chr8.3:72154197-72154216 MS.gene07830:CDS 50.0%
GTGCTTCACCATCAGTTCTG+AGG - chr8.3:72154759-72154778 None:intergenic 50.0%
TACTCGGGGGATCCTTTCTT+AGG - chr8.3:72156602-72156621 None:intergenic 50.0%
TCAACCTGAGAGCCACTGAA+CGG - chr8.3:72152939-72152958 None:intergenic 50.0%
TTAGCTCGTCCCGTTATTGG+AGG + chr8.3:72154137-72154156 MS.gene07830:CDS 50.0%
TTCAATCTTGGGCCGTTCAG+TGG + chr8.3:72152924-72152943 MS.gene07830:CDS 50.0%
! TCACTGTCCCCTTCACCTTT+TGG - chr8.3:72152792-72152811 None:intergenic 50.0%
!! GAGGAAGGATTGACTTGCAG+AGG + chr8.3:72156926-72156945 MS.gene07830:CDS 50.0%
!! TCGCTCCGTCTCTCACTTTT+AGG - chr8.3:72154496-72154515 None:intergenic 50.0%
!! TGCACTTGCAAGGAGTGTCT+TGG + chr8.3:72155837-72155856 MS.gene07830:CDS 50.0%
ACACCTTGCCTTACCCTCGA+AGG + chr8.3:72154165-72154184 MS.gene07830:CDS 55.0%
ACCTTGCCTTACCCTCGAAG+GGG + chr8.3:72154167-72154186 MS.gene07830:CDS 55.0%
ACGGCCCAAGATTGAACCGT+AGG - chr8.3:72152920-72152939 None:intergenic 55.0%
CACCTTGCCTTACCCTCGAA+GGG + chr8.3:72154166-72154185 MS.gene07830:CDS 55.0%
GCTAAGTAGCCCACATCCAC+AGG + chr8.3:72155485-72155504 MS.gene07830:CDS 55.0%
GCTTCAACGCCATCACTTGC+AGG - chr8.3:72155546-72155565 None:intergenic 55.0%
GGACCAAAGCTATCTGCCTG+TGG - chr8.3:72155504-72155523 None:intergenic 55.0%
TCCAGCTCCTAGAATCGGTG+CGG - chr8.3:72153660-72153679 None:intergenic 55.0%
TCGAGGGTAAGGCAAGGTGT+TGG - chr8.3:72154165-72154184 None:intergenic 55.0%
! GTGCGTGCATTTTACGAGGG+TGG + chr8.3:72154673-72154692 MS.gene07830:CDS 55.0%
!! GTTACCTCCGCACCGATTCT+AGG + chr8.3:72153650-72153669 MS.gene07830:CDS 55.0%
AGCTCCTAGAATCGGTGCGG+AGG - chr8.3:72153657-72153676 None:intergenic 60.0%
ATCAGAAGGCGAAGGCCGTG+TGG - chr8.3:72156248-72156267 None:intergenic 60.0%
CCTTGCCTTACCCTCGAAGG+GGG + chr8.3:72154168-72154187 MS.gene07830:CDS 60.0%
CGGCCTTCGCCTTCTGATAG+AGG + chr8.3:72156250-72156269 MS.gene07830:CDS 60.0%
GATATCAACGCCCTCACCGC+TGG + chr8.3:72152835-72152854 MS.gene07830:CDS 60.0%
GTTCTCCCCCTTCGAGGGTA+AGG - chr8.3:72154176-72154195 None:intergenic 60.0%
TACCCTCGAAGGGGGAGAAC+CGG + chr8.3:72154176-72154195 MS.gene07830:CDS 60.0%
TCAGAAGGCGAAGGCCGTGT+GGG - chr8.3:72156247-72156266 None:intergenic 60.0%
!! TCCGCACCGATTCTAGGAGC+TGG + chr8.3:72153656-72153675 MS.gene07830:CDS 60.0%
CAGAAGGCGAAGGCCGTGTG+GGG - chr8.3:72156246-72156265 None:intergenic 65.0%
CCCCCTTCGAGGGTAAGGCA+AGG - chr8.3:72154171-72154190 None:intergenic 65.0%
CGAAGGCCGTGTGGGGAAGT+AGG - chr8.3:72156239-72156258 None:intergenic 65.0%
TGGGCCGTTCAGTGGCTCTC+AGG + chr8.3:72152932-72152951 MS.gene07830:CDS 65.0%
!! TGCCGGTTCTCCCCCTTCGA+GGG - chr8.3:72154181-72154200 None:intergenic 65.0%
CTCGAAGGGGGAGAACCGGC+AGG + chr8.3:72154180-72154199 MS.gene07830:CDS 70.0%
!! CTGCCGGTTCTCCCCCTTCG+AGG - chr8.3:72154182-72154201 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 72152763 72156973 72152763 ID=MS.gene07830
chr8.3 mRNA 72152763 72156973 72152763 ID=MS.gene07830.t1;Parent=MS.gene07830
chr8.3 exon 72152763 72152979 72152763 ID=MS.gene07830.t1.exon1;Parent=MS.gene07830.t1
chr8.3 CDS 72152763 72152979 72152763 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72153603 72153889 72153603 ID=MS.gene07830.t1.exon2;Parent=MS.gene07830.t1
chr8.3 CDS 72153603 72153889 72153603 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72153978 72154218 72153978 ID=MS.gene07830.t1.exon3;Parent=MS.gene07830.t1
chr8.3 CDS 72153978 72154218 72153978 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72154491 72154811 72154491 ID=MS.gene07830.t1.exon4;Parent=MS.gene07830.t1
chr8.3 CDS 72154491 72154811 72154491 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72154900 72154985 72154900 ID=MS.gene07830.t1.exon5;Parent=MS.gene07830.t1
chr8.3 CDS 72154900 72154985 72154900 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72155110 72155217 72155110 ID=MS.gene07830.t1.exon6;Parent=MS.gene07830.t1
chr8.3 CDS 72155110 72155217 72155110 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72155333 72155637 72155333 ID=MS.gene07830.t1.exon7;Parent=MS.gene07830.t1
chr8.3 CDS 72155333 72155637 72155333 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72155720 72155983 72155720 ID=MS.gene07830.t1.exon8;Parent=MS.gene07830.t1
chr8.3 CDS 72155720 72155983 72155720 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
chr8.3 exon 72156217 72156973 72156217 ID=MS.gene07830.t1.exon9;Parent=MS.gene07830.t1
chr8.3 CDS 72156217 72156973 72156217 ID=cds.MS.gene07830.t1;Parent=MS.gene07830.t1
Gene Sequence

>MS.gene07830

ATGTTTTCAAATCCATTTCACAGAAGCCAAAAGGTGAAGGGGACAGTGATATTGATGCATAGGAATGTGTTGGATATCAACGCCCTCACCGCTGGTTTAAATATCACAGGTGGATTCAAGGTTCTTGGTAATCTCACTAGCTCTATATTTGACACCTACGGTTCAATCTTGGGCCGTTCAGTGGCTCTCAGGTTGATTAGTGCTACTAGTGCTGATGGTAATTACATTTTTTTTTTACTTATAATTACTTAATCTCTCACTCTACTGTCTACAAACATAATGTTCACCACATGGATGTATACTTAACGGGTATTGTTAACTTGTGCTCTAAGGACATGTTAAACAATCCTAAAATAGAAATATTCCATTAATATTTGTGCATTCAACTAATTAAAAGTTAACAAATTAAATGCAATACACGAATTCTAACAAAAAGTTTTTACATTTGACTCATTAATCTGTGACTTGAGGGCACAAGTTAACATTTCCGGATACTCGTAACTCATTGTTATAAAACCTCTTTCTTTTTTGATACAAATATACTGTTACACTTCAATTTGCGAATATACAGTAAAAGTTCTCATATTTTAAACTCACAATTGGTTTCTATGTTTAGAGAACGTCAAATATAAACATGTGGTTCAAAGATAGACTTTACTATTTTTTTACTAAACTTTAATAAAATATTACACATGTTCTTCATTTTTTACAATTACTATAATTTCTTTAAAAAATTGTTTTAAGAGTATTTGTTAATTTTTCATTTGAGAATTATCATAATTTTTTTTTTTTTATAAATCTTTACTGAAATTGAAAAACAAACTTGTGACATATTTGTAGAAAGTGGAAAAGGTAAAGTGGGAAAGAGATCGTTTCTTGAAGGTTTTGTTACCTCCGCACCGATTCTAGGAGCTGGACAGTGTGCATACAAAGTTCATTTTCAATGGGATAGTGACATGGGAATTCCAGGAGCATTTTATATTGAGAATTTCATGCTAGGAGAATTTTTTCTTGTAAGTTTAACACTTGAAGATATTCCTAACCATGGAACCATCAACTTTGTTTGCAACTCATGGATTTACAACTGTCGAAAGTACAAAACTGAACGCATTTTCTTTGCCAACAAGGTAATTATGAAAAATATCATATTTATCTTACATCTTAAATATCTTACATTTTCCAAAATTTGACACTATTAAGTAAATATAAACATAGACATATCTTCCAAGTGAAACACCAGCTCTATTAGTCTACTATAGACAAGAAGAACTGAAGACTTTACGAGGAGATGGGACAGGTAAACGTGAAGAATGGGAAAGGATCTATGACTATGATGTCTACAATGATGTGGGTGATCCTGATAGAAAAGCTAGCTTAGCTCGTCCCGTTATTGGAGGATCCAACACCTTGCCTTACCCTCGAAGGGGGAGAACCGGCAGGAAACCAGCTAAGAAAGGTTATAAACTTTTATATTCTATTTAAACGATCAAGTTAGTATAGTATGTTTTAAGGTATTTCTGCACAAAGGAAAGTGATATGGTACAACTATATGAAATATTAATGTAACTATATAGTTAATGTTTTCATCCCTAGTATAGGGTATGTTATGATGGCATTATTTTTTTGGTTGAAAATGTGTCTTTATGTTGATCAAAATGTTTCATTTGTTTATGATTCTAAAGTCATTCTTCTTGCTAATGCATATTTTGCTATTTTGTATCTGAGCAGATCCTAAAAGTGAGAGACGGAGCGAGTATATTTATCTTCCAAGAGATGAATCATTTGGTCACTTGAAGTCTTCAGACTTTCTTGTTTACATACTTAAATCAGTATCTCAGAATGTCATACCTACATTAACATCTGCAGTTACATTACAATTCAACAAGCCAGAGTTTAACACCTTTGATGATGTGCGTGCATTTTACGAGGGTGGAATTAAATTGCATACTAATGAACTTAGCAAACTTAGCCCTATACCATTGTTCAAGGAACTCCTCAGAACTGATGGTGAAGCACCCCTTAAGTTTCCACCACCTAAAATCATTCAAGGTATCCATTAGAAACACATTTTTAGTCATGCAAAATTTGGAAAGAAATTAATTTCATAGATCTTTTATATTTCTCTTATTTTGAAAAGTGAATCAATCTGGATGGATGACTGATGAAGAATTTACAAGAGAGATGATTGCTGGTGTGAATCCTCACATTATCAAAAGAATTCAGGTAAACTTCATAGTTTAATATAATTGTTATGCAAACACCAAAACTAAAATAAGAATATTGTATCCATTTTTCATCCTAGTTTTTTAATTGTTAACCTTATTATTGCTTGATATATTTAATGTAGGAATTTCCACCAAAGAGCAAGCTAGATAGGCAACTCTACGGTGATAATACCAGCACAATAACCAAAGAACAGCTTCAACAAAACATGGGTGGGATCACAGTTGAACAAGTAATTACAAAATTTAACATCATTACTCAAAATAAATAATTTTGTTATTGTATTGCAAAATTGTTTCATTTTCATTCAATTTATTGCAATGACTAAATTATATATTTTTTAATAGGCTATCGAAACCAACAAATTGTACATACTTGATCACCATGACTCAATATATCCATATCTGAGGAAAATAAATGGAGCTGATACAAAGGCATATGCTACGAGAACCTTCCTTTTCTTACAAAATGATGGAACTTTGAAGCCGTTGGCCATTGAGCTAAGTAGCCCACATCCACAGGCAGATAGCTTTGGTCCTGTTAGTGATATTTACTTGCCTGCAAGTGATGGCGTTGAAGCTTCAATTTGGCTCCTTGCCAAGGCTTATGTAGTTGTAAACGACTCTTGCCATCACCAACTCATCAGCCATTGGTATAAATTCAATTGACAAGATTATAGAGAGTAAAACATACTTTATATATGAGTTATCCGTTAATATCTATATGTGTGACAGGTTGAACACTCATGCTGTTGTTGAGCCATTCATCATAGCAACAAATAGGCATCTTAGTGTGGTTCATCCTATTCATAAGCTTTTACTTCCGCACTATCGTGACACAATGAACATTAATGCACTTGCAAGGAGTGTCTTGGTAAATGCAGAGGGTGTTATTGAGAAGACGTTCTTGATGGGAAGTTATTCTCTAGAATTGTCTGCTGTATTATATAAGGATTGGGTTTTCACTGAGCAAGGTTTGCCTAATGATCTACTCAAAAGGTAACCTTACAAAACATTAGATCCTAGACATATATTCAGTAGTTAAAAAGAGGATTGAAAATTTGTTCGCAACCTCAATCTAAGATGGGATATTCAGGTTTTTAATGTCTAAACATCAATGGTTGTGGTCGCATCAACTGTATTTTGCCGCAATTTTAAACTTTCCGTTTATAATATTGATATCAAAAGTTTTTATTTTGATATTAATGATATCTTCCTTTCTTGTTATCTAGAGGTGTGGCTCTTAAGGATCCTACTTCCCCACACGGCCTTCGCCTTCTGATAGAGGACTACCCTTATGCTGCAGATGGATTAGAGATATGGGCTGTTATTAAGTCATGGGTTGAAGAATATGTGAATTTCTACTACAAGTCAGATGTAAATATTGCACAAGATTCTGAACTCCAAGCATTTTGGAAAGAACTTGTAGAGGTTGGTCACGGTGACTTGAAAAATGCAACATGGTGGGTTAAGATGCAAACTCGTACAGAGTTGATTGACACTTGCACCACCCTCATATGGATTGCTTCAGCACTTCATGCAGCTGTTAATTTTGGACAATATCCGTACGGAGGTTACATTCTTAACCGACCAACTAAAAGCAGAAGATTAATGCCTAAGAAAGGATCCCCCGAGTATGATGAGCTTTCTAAGAACTATCAAAAGGCGTTTTTGAGAACAATCACACCAAAAAATGACACCCTAATTGACTTGACCATTATAGAGGTCTTGTCAAGACATGCTTCTGATGAACAATACCTCGGACAGAGAATTGAAGGTGATCTTTGGACTTCTGATTCACAACCATTAGAGGCTTTCAAGAGATTTGGAACAAAGTTGGCTGAAATTGAACAAAAACTCACTCAAAGGAACAATGATGAGACTCTAAGGAATCGATATGGACCGGTAAAGATGCCGTATACTTTGCTTTATCCTTCAAGTGAGGAAGGATTGACTTGCAGAGGCATTCCAAATAGTATCTCCATCTAA
Protein sequence

>MS.gene07830.t1

MFSNPFHRSQKVKGTVILMHRNVLDINALTAGLNITGGFKVLGNLTSSIFDTYGSILGRSVALRLISATSADESGKGKVGKRSFLEGFVTSAPILGAGQCAYKVHFQWDSDMGIPGAFYIENFMLGEFFLVSLTLEDIPNHGTINFVCNSWIYNCRKYKTERIFFANKTYLPSETPALLVYYRQEELKTLRGDGTGKREEWERIYDYDVYNDVGDPDRKASLARPVIGGSNTLPYPRRGRTGRKPAKKDPKSERRSEYIYLPRDESFGHLKSSDFLVYILKSVSQNVIPTLTSAVTLQFNKPEFNTFDDVRAFYEGGIKLHTNELSKLSPIPLFKELLRTDGEAPLKFPPPKIIQVNQSGWMTDEEFTREMIAGVNPHIIKRIQEFPPKSKLDRQLYGDNTSTITKEQLQQNMGGITVEQAIETNKLYILDHHDSIYPYLRKINGADTKAYATRTFLFLQNDGTLKPLAIELSSPHPQADSFGPVSDIYLPASDGVEASIWLLAKAYVVVNDSCHHQLISHWLNTHAVVEPFIIATNRHLSVVHPIHKLLLPHYRDTMNINALARSVLVNAEGVIEKTFLMGSYSLELSAVLYKDWVFTEQGLPNDLLKRGVALKDPTSPHGLRLLIEDYPYAADGLEIWAVIKSWVEEYVNFYYKSDVNIAQDSELQAFWKELVEVGHGDLKNATWWVKMQTRTELIDTCTTLIWIASALHAAVNFGQYPYGGYILNRPTKSRRLMPKKGSPEYDELSKNYQKAFLRTITPKNDTLIDLTIIEVLSRHASDEQYLGQRIEGDLWTSDSQPLEAFKRFGTKLAEIEQKLTQRNNDETLRNRYGPVKMPYTLLYPSSEEGLTCRGIPNSISI