AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07905


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07905.t1 MTR_8g020610 94.686 207 11 0 1 207 1 207 1.24e-142 396
MS.gene07905.t1 MTR_8g020590 58.373 209 84 2 1 207 1 208 1.11e-80 239
MS.gene07905.t1 MTR_8g020630 56.459 209 87 3 1 207 3 209 1.47e-76 229
MS.gene07905.t1 MTR_8g020670 56.796 206 85 3 4 207 6 209 4.45e-76 228
MS.gene07905.t1 MTR_2g041570 51.196 209 98 2 1 207 4 210 4.72e-73 220
MS.gene07905.t1 MTR_8g020650 58.333 192 76 3 18 207 20 209 9.10e-73 219
MS.gene07905.t1 MTR_2g041580 51.196 209 98 2 1 207 4 210 2.09e-72 218
MS.gene07905.t1 MTR_2g044040 54.067 209 92 3 1 207 3 209 3.13e-72 218
MS.gene07905.t1 MTR_2g041550 51.196 209 98 2 1 207 4 210 9.73e-72 217
MS.gene07905.t1 MTR_2g045100 53.883 206 91 3 4 207 6 209 2.14e-70 213
MS.gene07905.t1 MTR_3g019710 54.545 209 91 3 1 207 3 209 5.18e-70 212
MS.gene07905.t1 MTR_2g036120 51.923 208 96 3 1 206 3 208 6.10e-68 207
MS.gene07905.t1 MTR_8g020770 50.725 207 98 3 4 207 7 212 1.91e-66 203
MS.gene07905.t1 MTR_0284s0010 52.632 209 95 3 1 207 3 209 6.02e-66 202
MS.gene07905.t1 MTR_3g019650 52.427 206 93 4 4 207 6 208 2.81e-65 200
MS.gene07905.t1 MTR_4g010340 52.913 206 90 5 4 207 5 205 3.95e-65 200
MS.gene07905.t1 MTR_5g031460 50.239 209 100 3 1 207 3 209 2.23e-64 198
MS.gene07905.t1 MTR_3g019680 51.942 206 94 4 4 207 6 208 4.06e-64 197
MS.gene07905.t1 MTR_7g011340 53.093 194 87 3 16 207 18 209 3.38e-61 190
MS.gene07905.t1 MTR_3g073170 51.337 187 87 4 22 207 27 210 1.84e-59 186
MS.gene07905.t1 MTR_2g019780 49.048 210 100 5 2 207 6 212 1.91e-59 186
MS.gene07905.t1 MTR_8g020760 49.519 208 101 3 2 207 6 211 4.97e-58 182
MS.gene07905.t1 MTR_8g020750 45.894 207 108 3 4 207 7 212 1.06e-57 181
MS.gene07905.t1 MTR_3g019810 45.813 203 100 4 1 201 109 303 1.04e-51 169
MS.gene07905.t1 MTR_8g020763 46.995 183 94 2 27 207 9 190 1.61e-51 164
MS.gene07905.t1 MTR_1g060120 37.500 192 106 5 20 200 30 218 1.30e-36 127
MS.gene07905.t1 MTR_1g085140 37.838 185 109 4 22 201 30 213 2.27e-34 122
MS.gene07905.t1 MTR_4g027415 49.606 127 60 3 16 140 18 142 6.17e-33 115
MS.gene07905.t1 MTR_2g072560 51.351 111 51 2 4 112 6 115 7.80e-32 113
MS.gene07905.t1 MTR_1g112200 34.737 190 115 4 20 206 27 210 8.90e-32 115
MS.gene07905.t1 MTR_7g111240 37.975 158 96 1 22 177 26 183 1.21e-30 112
MS.gene07905.t1 MTR_6g005360 39.474 190 105 6 22 203 28 215 2.71e-30 111
MS.gene07905.t1 MTR_6g005330 39.474 190 105 6 22 203 28 215 3.98e-30 110
MS.gene07905.t1 MTR_6g005310 39.474 190 105 6 22 203 28 215 5.09e-30 110
MS.gene07905.t1 MTR_7g111240 37.975 158 93 2 22 177 26 180 1.38e-29 109
MS.gene07905.t1 MTR_5g036570 33.333 189 121 3 22 205 33 221 1.86e-29 109
MS.gene07905.t1 MTR_1g079490 37.838 185 104 5 3 177 6 189 2.26e-29 108
MS.gene07905.t1 MTR_6g005340 38.947 190 106 6 22 203 28 215 3.29e-29 108
MS.gene07905.t1 MTR_2g031270 35.638 188 112 4 22 203 29 213 1.35e-28 107
MS.gene07905.t1 MTR_2g030855 34.555 191 116 4 19 203 25 212 4.08e-28 105
MS.gene07905.t1 MTR_1g023770 35.468 203 118 5 8 203 13 209 6.01e-28 105
MS.gene07905.t1 MTR_1g085500 32.240 183 120 2 22 200 29 211 6.36e-28 105
MS.gene07905.t1 MTR_3g019610 50.000 102 48 2 4 103 6 106 3.48e-27 100
MS.gene07905.t1 MTR_2g019250 34.286 210 125 7 1 200 7 213 5.96e-27 102
MS.gene07905.t1 MTR_4g017040 35.829 187 109 6 1 179 7 190 7.60e-27 102
MS.gene07905.t1 MTR_4g017050 33.810 210 126 7 1 200 7 213 1.08e-26 102
MS.gene07905.t1 MTR_5g046410 33.810 210 126 7 1 200 7 213 1.29e-26 102
MS.gene07905.t1 MTR_5g046430 33.810 210 126 7 1 200 7 213 1.29e-26 102
MS.gene07905.t1 MTR_4g017030 33.810 210 126 7 1 200 7 213 1.29e-26 102
MS.gene07905.t1 MTR_2g030895 35.465 172 104 4 39 203 7 178 3.50e-25 97.1
MS.gene07905.t1 MTR_8g007300 32.308 195 126 4 2 191 11 204 5.06e-25 97.8
MS.gene07905.t1 MTR_6g005380 36.571 175 101 6 1 167 6 178 2.56e-24 95.5
MS.gene07905.t1 MTR_2g030865 34.884 172 105 4 39 203 7 178 1.15e-23 93.2
MS.gene07905.t1 MTR_3g096330 37.255 153 77 3 29 167 584 731 1.98e-22 94.7
MS.gene07905.t1 MTR_6g005350 33.333 189 117 6 22 203 28 214 2.06e-21 88.2
MS.gene07905.t1 MTR_2g086620 32.353 170 109 3 38 203 37 204 3.45e-20 84.7
MS.gene07905.t1 MTR_2g086630 31.765 170 110 3 38 203 37 204 1.20e-19 83.2
MS.gene07905.t1 MTR_4g052780 33.136 169 110 2 38 203 37 205 3.31e-19 82.0
MS.gene07905.t1 MTR_4g052770 33.136 169 110 2 38 203 37 205 4.35e-19 81.6
MS.gene07905.t1 MTR_2g086640 34.074 135 88 1 70 203 69 203 5.55e-19 81.6
MS.gene07905.t1 MTR_2g031300 32.500 160 82 4 39 192 7 146 1.38e-16 73.9
MS.gene07905.t1 MTR_2g096110 30.469 128 73 2 41 167 7 119 1.39e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07905.t1 AT1G72610 53.368 193 88 1 17 207 16 208 5.13e-72 218
MS.gene07905.t1 AT5G20630 50.000 208 101 2 1 206 4 210 2.64e-68 208
MS.gene07905.t1 AT1G18980 37.073 205 125 2 3 203 14 218 1.10e-42 143
MS.gene07905.t1 AT1G18970 35.122 205 129 2 1 203 16 218 2.52e-39 134
MS.gene07905.t1 AT3G04200 40.838 191 105 4 22 204 33 223 8.38e-39 133
MS.gene07905.t1 AT5G26700 37.438 203 117 5 4 200 8 206 7.58e-35 123
MS.gene07905.t1 AT3G05930 37.363 182 110 3 2 179 8 189 8.05e-35 123
MS.gene07905.t1 AT1G09560 36.022 186 115 2 22 203 30 215 2.87e-33 119
MS.gene07905.t1 AT5G38960 37.306 193 110 7 22 204 32 223 6.10e-32 115
MS.gene07905.t1 AT3G10080 34.715 193 114 5 20 203 30 219 1.47e-31 115
MS.gene07905.t1 AT1G02335 35.443 158 100 1 22 177 30 187 1.76e-31 114
MS.gene07905.t1 AT3G04150 34.112 214 133 4 2 207 12 225 2.13e-31 114
MS.gene07905.t1 AT3G10080 34.715 193 114 5 20 203 101 290 8.28e-31 114
MS.gene07905.t1 AT3G04150 32.159 227 133 4 2 207 12 238 2.34e-30 112
MS.gene07905.t1 AT3G62020 30.918 207 138 3 3 204 10 216 2.55e-30 111
MS.gene07905.t1 AT3G05950 37.895 190 110 4 22 203 32 221 3.10e-30 111
MS.gene07905.t1 AT5G61750 34.091 176 100 3 2 167 5 174 3.23e-30 110
MS.gene07905.t1 AT5G39110 34.579 214 127 5 3 203 7 220 1.54e-29 109
MS.gene07905.t1 AT1G74820 32.558 172 110 1 1 166 15 186 1.77e-29 109
MS.gene07905.t1 AT5G38910 33.971 209 128 5 4 203 12 219 1.70e-28 107
MS.gene07905.t1 AT3G04190 36.649 191 111 6 22 203 32 221 2.57e-28 106
MS.gene07905.t1 AT5G38940 34.737 190 116 4 22 203 32 221 3.62e-28 105
MS.gene07905.t1 AT5G39160 34.896 192 111 5 22 204 30 216 4.34e-28 105
MS.gene07905.t1 AT5G38940 34.737 190 116 4 22 203 56 245 6.28e-28 105
MS.gene07905.t1 AT5G39160 34.896 192 115 5 22 204 30 220 9.52e-28 105
MS.gene07905.t1 AT5G39190 34.896 192 115 5 22 204 30 220 1.15e-27 104
MS.gene07905.t1 AT5G38930 34.737 190 116 5 22 203 32 221 1.79e-27 104
MS.gene07905.t1 AT5G39120 32.243 214 133 6 1 203 7 219 2.75e-27 103
MS.gene07905.t1 AT5G39130 33.488 215 129 6 3 204 7 220 1.61e-26 101
MS.gene07905.t1 AT5G39150 30.986 213 136 5 1 203 8 219 2.67e-26 101
MS.gene07905.t1 AT3G62020 31.609 174 114 3 35 203 13 186 3.02e-26 100
MS.gene07905.t1 AT5G39180 30.986 213 136 5 1 203 8 219 3.92e-26 100
MS.gene07905.t1 AT3G04180 35.204 196 107 7 22 203 32 221 1.01e-25 99.8
MS.gene07905.t1 AT4G14630 34.762 210 125 7 5 203 14 222 1.26e-25 99.4
MS.gene07905.t1 AT3G04170 36.082 194 105 8 22 203 32 218 1.57e-24 96.7
MS.gene07905.t1 AT1G10460 34.568 162 99 4 22 176 25 186 6.16e-24 94.7
MS.gene07905.t1 AT5G39160 35.065 154 94 3 56 204 46 198 2.13e-23 93.2
MS.gene07905.t1 AT5G39190 35.065 154 94 3 56 204 46 198 2.20e-23 93.2

Find 28 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAATTTCCTGCTGTTAA+TGG 0.167755 8.3:-70537608 MS.gene07905:CDS
GTTTACTCCAACTTCAATTC+AGG 0.252771 8.3:-70537680 MS.gene07905:CDS
ATTCTTGATCTTGCTTTGTT+TGG 0.254800 8.3:-70537317 MS.gene07905:CDS
ACTTTCTAAAGGTCAAATTT+TGG 0.284643 8.3:-70537435 MS.gene07905:CDS
TTTCCTGCTGTTAATGGTCT+TGG 0.310444 8.3:-70537602 MS.gene07905:CDS
TCTTCTCCACAAACCCCTTC+AGG 0.334680 8.3:-70537740 MS.gene07905:CDS
TGGTTTATCAGCAGCAAGAT+TGG 0.394978 8.3:-70537582 MS.gene07905:CDS
TAATACAGAAGCATTGGTTA+TGG 0.446790 8.3:+70537784 None:intergenic
GGGTAGCCTGAAGGGGTTTG+TGG 0.451614 8.3:+70537734 None:intergenic
GCATTCACATCGCGGCGCTT+CGG 0.455830 8.3:-70537531 MS.gene07905:CDS
GGAAATTGATTAACAGATGC+AGG 0.477175 8.3:+70537620 None:intergenic
TTTATCAAGACACTTTCTAA+AGG 0.499284 8.3:-70537446 MS.gene07905:CDS
GCAAGATTGGACTTCGAGGT+TGG 0.499476 8.3:-70537569 MS.gene07905:CDS
CGCTTCGGAGTTAATGATTG+TGG 0.511937 8.3:-70537516 MS.gene07905:CDS
GAAGTCTAATACAGAAGCAT+TGG 0.518908 8.3:+70537778 None:intergenic
AGTGTTTCCTGAATTGAAGT+TGG 0.520987 8.3:+70537673 None:intergenic
GTACAAGGTCGTATAACTGC+TGG 0.537303 8.3:-70537494 MS.gene07905:CDS
AGATCAAGAATTTGTGAACC+AGG 0.540464 8.3:+70537329 None:intergenic
TTTGCATGGGTAGCCTGAAG+GGG 0.553451 8.3:+70537727 None:intergenic
AGATTGGACTTCGAGGTTGG+AGG 0.573052 8.3:-70537566 MS.gene07905:CDS
GCGCCGCGATGTGAATGCAT+TGG 0.582043 8.3:+70537536 None:intergenic
AGCAGCAAGATTGGACTTCG+AGG 0.583902 8.3:-70537573 MS.gene07905:CDS
AAACCAAGACCATTAACAGC+AGG 0.584052 8.3:+70537599 None:intergenic
GAGTTAATGATTGTGGTACA+AGG 0.597384 8.3:-70537509 MS.gene07905:CDS
GATCAAGAATTTGTGAACCA+GGG 0.620980 8.3:+70537330 None:intergenic
AATGTGAGAGAAGAGTTAAG+AGG 0.621704 8.3:+70537807 None:intergenic
TGAAAATGCAACAAACCTTG+TGG 0.665243 8.3:+70537407 None:intergenic
ATACCAATGCATTCACATCG+CGG 0.682883 8.3:-70537539 MS.gene07905:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTATCAAGACACTTTCTAA+AGG - chr8.3:70537601-70537620 MS.gene07905:CDS 25.0%
!! ATTTTCAATATAATGCTGGA+AGG - chr8.3:70537656-70537675 MS.gene07905:CDS 25.0%
!! TTGCATTTTCAATATAATGC+TGG - chr8.3:70537652-70537671 MS.gene07905:CDS 25.0%
!!! AAAAAGCTTAAGGCTATTTT+TGG - chr8.3:70537811-70537830 MS.gene07905:CDS 25.0%
!!! ACTTTCTAAAGGTCAAATTT+TGG - chr8.3:70537612-70537631 MS.gene07905:CDS 25.0%
!!! ATTGTTACTGATTTTTTGCA+TGG + chr8.3:70537337-70537356 None:intergenic 25.0%
!!! TTGTTACTGATTTTTTGCAT+GGG + chr8.3:70537336-70537355 None:intergenic 25.0%
AATCAATTTCCTGCTGTTAA+TGG - chr8.3:70537439-70537458 MS.gene07905:CDS 30.0%
TGCTCAAGTTAAAAAGCTTA+AGG - chr8.3:70537801-70537820 MS.gene07905:CDS 30.0%
! ATTCTTGATCTTGCTTTGTT+TGG - chr8.3:70537730-70537749 MS.gene07905:CDS 30.0%
!! TAATACAGAAGCATTGGTTA+TGG + chr8.3:70537266-70537285 None:intergenic 30.0%
!!! TTAAGCTTTTTAACTTGAGC+AGG + chr8.3:70537802-70537821 None:intergenic 30.0%
AATGTGAGAGAAGAGTTAAG+AGG + chr8.3:70537243-70537262 None:intergenic 35.0%
AGATCAAGAATTTGTGAACC+AGG + chr8.3:70537721-70537740 None:intergenic 35.0%
AGTGTTTCCTGAATTGAAGT+TGG + chr8.3:70537377-70537396 None:intergenic 35.0%
GAGTTAATGATTGTGGTACA+AGG - chr8.3:70537538-70537557 MS.gene07905:CDS 35.0%
GATCAAGAATTTGTGAACCA+GGG + chr8.3:70537720-70537739 None:intergenic 35.0%
GGAAATTGATTAACAGATGC+AGG + chr8.3:70537430-70537449 None:intergenic 35.0%
GTTTACTCCAACTTCAATTC+AGG - chr8.3:70537367-70537386 MS.gene07905:CDS 35.0%
TGAAAATGCAACAAACCTTG+TGG + chr8.3:70537643-70537662 None:intergenic 35.0%
! GAAGTCTAATACAGAAGCAT+TGG + chr8.3:70537272-70537291 None:intergenic 35.0%
!!! AAGCTTAAGGCTATTTTTGG+TGG - chr8.3:70537814-70537833 MS.gene07905:CDS 35.0%
!!! AGCTTAAGGCTATTTTTGGT+GGG - chr8.3:70537815-70537834 MS.gene07905:CDS 35.0%
AAACCAAGACCATTAACAGC+AGG + chr8.3:70537451-70537470 None:intergenic 40.0%
ATACCAATGCATTCACATCG+CGG - chr8.3:70537508-70537527 MS.gene07905:CDS 40.0%
TGGTTTATCAGCAGCAAGAT+TGG - chr8.3:70537465-70537484 MS.gene07905:CDS 40.0%
TTTCCTGCTGTTAATGGTCT+TGG - chr8.3:70537445-70537464 MS.gene07905:CDS 40.0%
! ACTTTTAGTAGTGAGAACCC+TGG - chr8.3:70537700-70537719 MS.gene07905:CDS 40.0%
!!! CAAATTTTGGCTCTACCACA+AGG - chr8.3:70537625-70537644 MS.gene07905:CDS 40.0%
CGCTTCGGAGTTAATGATTG+TGG - chr8.3:70537531-70537550 MS.gene07905:CDS 45.0%
GTACAAGGTCGTATAACTGC+TGG - chr8.3:70537553-70537572 MS.gene07905:CDS 45.0%
! TTTTGCATGGGTAGCCTGAA+GGG + chr8.3:70537324-70537343 None:intergenic 45.0%
! TTTTTGCATGGGTAGCCTGA+AGG + chr8.3:70537325-70537344 None:intergenic 45.0%
!!! AAGGCTATTTTTGGTGGGAG+TGG - chr8.3:70537820-70537839 MS.gene07905:CDS 45.0%
AGATTGGACTTCGAGGTTGG+AGG - chr8.3:70537481-70537500 MS.gene07905:CDS 50.0%
AGCAGCAAGATTGGACTTCG+AGG - chr8.3:70537474-70537493 MS.gene07905:CDS 50.0%
GCAAGATTGGACTTCGAGGT+TGG - chr8.3:70537478-70537497 MS.gene07905:CDS 50.0%
TCTTCTCCACAAACCCCTTC+AGG - chr8.3:70537307-70537326 MS.gene07905:CDS 50.0%
TTTGCATGGGTAGCCTGAAG+GGG + chr8.3:70537323-70537342 None:intergenic 50.0%
GCATTCACATCGCGGCGCTT+CGG - chr8.3:70537516-70537535 MS.gene07905:CDS 60.0%
GCGCCGCGATGTGAATGCAT+TGG + chr8.3:70537514-70537533 None:intergenic 60.0%
GGGTAGCCTGAAGGGGTTTG+TGG + chr8.3:70537316-70537335 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 70537223 70537846 70537223 ID=MS.gene07905
chr8.3 mRNA 70537223 70537846 70537223 ID=MS.gene07905.t1;Parent=MS.gene07905
chr8.3 exon 70537223 70537846 70537223 ID=MS.gene07905.t1.exon1;Parent=MS.gene07905.t1
chr8.3 CDS 70537223 70537846 70537223 ID=cds.MS.gene07905.t1;Parent=MS.gene07905.t1
Gene Sequence

>MS.gene07905

ATGATTTTCATTCTTTTCCTCTTAACTCTTCTCTCACATTCCATAACCAATGCTTCTGTATTAGACTTCTGTGTAGCAGATTTATCTTCTCCACAAACCCCTTCAGGCTACCCATGCAAAAAATCAGTAACAATCAATGACTTTGTTTACTCCAACTTCAATTCAGGAAACACTTCAGCAAATTTTTTCAAATTTTCAATAACTCCTGCATCTGTTAATCAATTTCCTGCTGTTAATGGTCTTGGTTTATCAGCAGCAAGATTGGACTTCGAGGTTGGAGGTATAATACCAATGCATTCACATCGCGGCGCTTCGGAGTTAATGATTGTGGTACAAGGTCGTATAACTGCTGGTTTTATTTCTTCTGATAATACTGTTTTTATCAAGACACTTTCTAAAGGTCAAATTTTGGCTCTACCACAAGGTTTGTTGCATTTTCAATATAATGCTGGAAGGAATAAAGCTTCTGCTTATCTTACTTTTAGTAGTGAGAACCCTGGTTCACAAATTCTTGATCTTGCTTTGTTTGGAAATAATTTGAGTTCTGCTAATGTGATAAAGAGTACTTTGCTTGATCCTGCTCAAGTTAAAAAGCTTAAGGCTATTTTTGGTGGGAGTGGCTAG

Protein sequence

>MS.gene07905.t1

MIFILFLLTLLSHSITNASVLDFCVADLSSPQTPSGYPCKKSVTINDFVYSNFNSGNTSANFFKFSITPASVNQFPAVNGLGLSAARLDFEVGGIIPMHSHRGASELMIVVQGRITAGFISSDNTVFIKTLSKGQILALPQGLLHFQYNAGRNKASAYLTFSSENPGSQILDLALFGNNLSSANVIKSTLLDPAQVKKLKAIFGGSG