AlfalfaGEDB | MS.gene054138

M. sativa cultivar XinJiangDaYe / MS.gene054138

KEGG: -
GO: -
PFAM: PF06376.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054138.t1	XP_013466382.1	85.5	62	9	0	1	62	1	62	1.20E-08	68.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054138.t1	A0A072VG86	85.5	62	9	0	1	62	1	62	8.6e-09	68.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene054138	0.807492	4.92E-50	-1.69E-46
MS.gene052223	MS.gene054138	0.804649	1.95E-49	-1.69E-46
MS.gene054138	MS.gene054147	0.801883	7.30E-49	-1.69E-46
MS.gene054138	MS.gene054742	0.832151	1.11E-55	-1.69E-46
MS.gene054138	MS.gene056330	0.838997	2.06E-57	-1.69E-46
MS.gene054138	MS.gene056920	0.808846	2.53E-50	-1.69E-46
MS.gene054138	MS.gene056922	0.825743	3.97E-54	-1.69E-46
MS.gene054138	MS.gene056923	0.812999	3.20E-51	-1.69E-46
MS.gene054138	MS.gene056924	0.816967	4.21E-52	-1.69E-46
MS.gene054138	MS.gene057261	0.800517	1.39E-48	-1.69E-46
MS.gene054138	MS.gene059039	0.811392	7.16E-51	-1.69E-46
MS.gene054138	MS.gene060087	0.805734	1.16E-49	-1.69E-46
MS.gene054138	MS.gene063988	0.812525	4.06E-51	-1.69E-46
MS.gene054138	MS.gene064961	0.801306	9.58E-49	-1.69E-46
MS.gene054138	MS.gene065724	0.81998	8.73E-53	-1.69E-46
MS.gene054138	MS.gene065729	0.809066	2.27E-50	-1.69E-46
MS.gene054138	MS.gene06593	0.813008	3.18E-51	-1.69E-46
MS.gene054138	MS.gene066417	0.801036	1.09E-48	-1.69E-46
MS.gene054138	MS.gene066937	0.817193	3.74E-52	-1.69E-46
MS.gene054138	MS.gene06756	0.827937	1.19E-54	-1.69E-46
MS.gene054138	MS.gene06805	0.812676	3.76E-51	-1.69E-46
MS.gene054138	MS.gene071840	0.831386	1.72E-55	-1.69E-46
MS.gene054138	MS.gene074299	0.832612	8.56E-56	-1.69E-46
MS.gene054138	MS.gene07905	0.807321	5.35E-50	-1.69E-46
MS.gene054138	MS.gene23026	0.802263	6.10E-49	-1.69E-46
MS.gene054138	MS.gene23507	0.800227	1.59E-48	-1.69E-46
MS.gene054138	MS.gene25138	0.800467	1.42E-48	-1.69E-46
MS.gene054138	MS.gene25652	0.814312	1.64E-51	-1.69E-46
MS.gene054138	MS.gene26289	0.823298	1.49E-53	-1.69E-46
MS.gene054138	MS.gene28548	0.806472	8.09E-50	-1.69E-46
MS.gene054138	MS.gene30382	0.828953	6.75E-55	-1.69E-46
MS.gene054138	MS.gene32342	0.96247	6.47E-121	-1.69E-46
MS.gene054138	MS.gene35433	0.814565	1.44E-51	-1.69E-46
MS.gene054138	MS.gene35690	0.842164	3.06E-58	-1.69E-46
MS.gene054138	MS.gene40846	0.802873	4.56E-49	-1.69E-46
MS.gene054138	MS.gene42005	0.802721	4.91E-49	-1.69E-46
MS.gene054138	MS.gene42096	0.813667	2.28E-51	-1.69E-46
MS.gene054138	MS.gene43162	0.82018	7.86E-53	-1.69E-46
MS.gene054138	MS.gene43874	0.80871	2.71E-50	-1.69E-46
MS.gene054138	MS.gene44712	0.815679	8.17E-52	-1.69E-46
MS.gene054138	MS.gene44958	0.810255	1.26E-50	-1.69E-46
MS.gene054138	MS.gene48169	0.825098	5.64E-54	-1.69E-46
MS.gene054138	MS.gene51331	0.800113	1.68E-48	-1.69E-46
MS.gene054138	MS.gene54146	0.802791	4.74E-49	-1.69E-46
MS.gene054138	MS.gene54611	0.828556	8.42E-55	-1.69E-46
MS.gene054138	MS.gene60581	0.805744	1.15E-49	-1.69E-46
MS.gene054138	MS.gene65144	0.818331	2.07E-52	-1.69E-46
MS.gene054138	MS.gene65823	0.820339	7.23E-53	-1.69E-46
MS.gene054138	MS.gene67371	0.863212	2.93E-64	-1.69E-46
MS.gene054138	MS.gene67377	0.852443	4.60E-61	-1.69E-46
MS.gene054138	MS.gene71342	0.835734	1.41E-56	-1.69E-46
MS.gene054138	MS.gene71346	0.830975	2.17E-55	-1.69E-46
MS.gene054138	MS.gene73995	0.812257	4.64E-51	-1.69E-46
MS.gene054138	MS.gene75460	0.800691	1.28E-48	-1.69E-46
MS.gene054138	MS.gene79232	0.802699	4.96E-49	-1.69E-46
MS.gene054138	MS.gene80293	0.812924	3.32E-51	-1.69E-46
MS.gene054138	MS.gene80744	0.821372	4.18E-53	-1.69E-46
MS.gene054138	MS.gene81770	0.860951	1.44E-63	-1.69E-46
MS.gene054138	MS.gene81771	0.819497	1.13E-52	-1.69E-46
MS.gene054138	MS.gene81774	0.819609	1.06E-52	-1.69E-46
MS.gene054138	MS.gene84143	0.824526	7.69E-54	-1.69E-46
MS.gene054138	MS.gene85750	0.8354	1.72E-56	-1.69E-46
MS.gene054138	MS.gene87043	0.813126	3.00E-51	-1.69E-46
MS.gene054138	MS.gene88971	0.80442	2.18E-49	-1.69E-46
MS.gene054138	MS.gene91221	0.82913	6.11E-55	-1.69E-46
MS.gene054138	MS.gene91222	0.832058	1.17E-55	-1.69E-46
MS.gene054138	MS.gene91226	0.803995	2.67E-49	-1.69E-46
MS.gene054138	MS.gene94593	0.816997	4.14E-52	-1.69E-46
MS.gene054138	MS.gene97024	0.808122	3.62E-50	-1.69E-46
MS.gene054138	MS.gene97182	0.812447	4.22E-51	-1.69E-46
MS.gene054138	MS.gene97335	0.807831	4.17E-50	-1.69E-46
MS.gene054138	MS.gene98466	0.809861	1.53E-50	-1.69E-46
MS.gene054138	MS.gene98470	0.820353	7.18E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene55776	MS.gene054138	PPI
MS.gene048906	MS.gene054138	PPI
MS.gene06050	MS.gene054138	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054138.t1	MTR_1g028610	95.161	62	3	0	1	62	1	62	6.98e-29	97.4
MS.gene054138.t1	MTR_3g090830	77.966	59	11	1	4	62	7	63	9.14e-25	87.0
MS.gene054138.t1	MTR_6g086390	41.667	60	35	0	3	62	5	64	2.74e-11	53.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene054138.t1	AT2G46330	66.000	50	15	1	13	62	15	62	6.39e-16	65.1
MS.gene054138.t1	AT3G61640	57.143	56	22	1	7	62	9	62	1.68e-15	63.9
MS.gene054138.t1	AT5G53250	76.923	39	9	0	24	62	25	63	9.63e-15	61.6

Find 11 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGCAAGGCCACCAGCATT+AGG	0.444107	1.3:+14866867	None:intergenic
TCACTTGTGGGAGCAGGAGC+AGG	0.473725	1.3:+14867726	None:intergenic
TTCTTCACAGTCTGTTGCTC+CGG	0.477404	1.3:-14867751	MS.gene054138:CDS
GTGGGAGCAGGAGCAGGAGC+CGG	0.500195	1.3:+14867732	None:intergenic
TTAGGACATATGCTATTGCT+TGG	0.507960	1.3:+14866885	None:intergenic
TTACCATCACTTGTGGGAGC+AGG	0.532408	1.3:+14867720	None:intergenic
GCTCCTGCTCCCACAAGTGA+TGG	0.550760	1.3:-14867723	MS.gene054138:intron
AATAGCATATGTCCTAATGC+TGG	0.576700	1.3:-14866879	MS.gene054138:CDS
AGCATATGTCCTAATGCTGG+TGG	0.610729	1.3:-14866876	MS.gene054138:CDS
AAGATGCGTAAATAAGACCA+AGG	0.685667	1.3:+14867787	None:intergenic
AATGATGTAAGTGAGAAGCA+AGG	0.690125	1.3:+14866853	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATAAATCTAGACTATATT+GGG	-	chr1.3:14867416-14867435	MS.gene054138:intron	15.0%
!!	TCAATCAAAAACTTTAAATA+AGG	-	chr1.3:14867217-14867236	MS.gene054138:intron	15.0%
!!	TGTTATTATTAACTGATAAT+AGG	+	chr1.3:14867570-14867589	None:intergenic	15.0%
!!!	TAAGATTATGATTTTAACAT+AGG	-	chr1.3:14866974-14866993	MS.gene054138:intron	15.0%
!!!	TTTTGGTTTTTTTTTTTCAA+CGG	-	chr1.3:14867543-14867562	MS.gene054138:intron	15.0%
!!	ACCGAAAAATAATTAAATTG+TGG	+	chr1.3:14867518-14867537	None:intergenic	20.0%
!!	CAATATAGTCTAGATTTATT+AGG	+	chr1.3:14867417-14867436	None:intergenic	20.0%
!!	CATTTGATACAATATAACTA+TGG	+	chr1.3:14867686-14867705	None:intergenic	20.0%
!!	CTAATAAATCTAGACTATAT+TGG	-	chr1.3:14867415-14867434	MS.gene054138:intron	20.0%
!!	TCAAGAAAAATAACACAATT+AGG	+	chr1.3:14867071-14867090	None:intergenic	20.0%
!!!	AATTGTTCTTAAAATTTTGC+AGG	-	chr1.3:14867743-14867762	MS.gene054138:CDS	20.0%
!!!	AATTTTAATTATTAGCCATG+CGG	+	chr1.3:14867727-14867746	None:intergenic	20.0%
!!!	GCCACAATTTAATTATTTTT+CGG	-	chr1.3:14867514-14867533	MS.gene054138:intron	20.0%
!	ATACAATATAACTATGGAGT+GGG	+	chr1.3:14867680-14867699	None:intergenic	25.0%
!	TGATATAGTTCGATTCAAAA+AGG	-	chr1.3:14867593-14867612	MS.gene054138:intron	25.0%
!	TGTTTGTGAATTGTTAATCA+TGG	+	chr1.3:14867466-14867485	None:intergenic	25.0%
!	TTGTATCAAATGTTGTTGTA+TGG	-	chr1.3:14867694-14867713	MS.gene054138:intron	25.0%
	AAAAACTTACCATCACTTGT+GGG	+	chr1.3:14866952-14866971	None:intergenic	30.0%
	AAAAAGAGTTTCTCAACATG+AGG	+	chr1.3:14867180-14867199	None:intergenic	30.0%
	AATCCATGAACCATATAAGA+TGG	+	chr1.3:14867043-14867062	None:intergenic	30.0%
	GATACAATATAACTATGGAG+TGG	+	chr1.3:14867681-14867700	None:intergenic	30.0%
	TAAAAACTTACCATCACTTG+TGG	+	chr1.3:14866953-14866972	None:intergenic	30.0%
	TACAGTTCAACTTTCAGATA+TGG	-	chr1.3:14867616-14867635	MS.gene054138:intron	30.0%
!!	TTATTTTTCGGTGTGACTTT+TGG	-	chr1.3:14867526-14867545	MS.gene054138:intron	30.0%
	AAGATGCGTAAATAAGACCA+AGG	+	chr1.3:14866879-14866898	None:intergenic	35.0%
	AATAGCATATGTCCTAATGC+TGG	-	chr1.3:14867784-14867803	MS.gene054138:CDS	35.0%
	AATCATGGTTCCATTCTCAT+CGG	+	chr1.3:14867451-14867470	None:intergenic	35.0%
	AATGATGTAAGTGAGAAGCA+AGG	+	chr1.3:14867813-14867832	None:intergenic	35.0%
	GAATCACAGCTATACATGAT+TGG	+	chr1.3:14867321-14867340	None:intergenic	35.0%
	GCAATTTAGACTACAACATC+TGG	-	chr1.3:14867249-14867268	MS.gene054138:intron	35.0%
	GTTGATCTCACCATCTTATA+TGG	-	chr1.3:14867030-14867049	MS.gene054138:intron	35.0%
	TCACCATCTTATATGGTTCA+TGG	-	chr1.3:14867037-14867056	MS.gene054138:intron	35.0%
	TTAGGACATATGCTATTGCT+TGG	+	chr1.3:14867781-14867800	None:intergenic	35.0%
	CTTCAACCAGAATCACATTG+TGG	+	chr1.3:14867293-14867312	None:intergenic	40.0%
	TTGTATGGTTTCAAACCGCA+TGG	-	chr1.3:14867709-14867728	MS.gene054138:intron	40.0%
!	ATATGGATGCCTTTTGTAGG+AGG	-	chr1.3:14867633-14867652	MS.gene054138:intron	40.0%
!	CAGATATGGATGCCTTTTGT+AGG	-	chr1.3:14867630-14867649	MS.gene054138:intron	40.0%
!	TATGGATGCCTTTTGTAGGA+GGG	-	chr1.3:14867634-14867653	MS.gene054138:intron	40.0%
!	TTACGCATCTTTTGCGTCAA+TGG	-	chr1.3:14866888-14866907	MS.gene054138:CDS	40.0%
	AGCATATGTCCTAATGCTGG+TGG	-	chr1.3:14867787-14867806	MS.gene054138:CDS	45.0%
	CAGTGACCACAATGTGATTC+TGG	-	chr1.3:14867284-14867303	MS.gene054138:intron	45.0%
	GATGGTGAGATCAACATCGA+AGG	+	chr1.3:14867025-14867044	None:intergenic	45.0%
	TTCTTCACAGTCTGTTGCTC+CGG	-	chr1.3:14866912-14866931	MS.gene054138:intron	45.0%
!	ATGGATGCCTTTTGTAGGAG+GGG	-	chr1.3:14867635-14867654	MS.gene054138:intron	45.0%
!	GTTTCATGCACCGATGAGAA+TGG	-	chr1.3:14867438-14867457	MS.gene054138:intron	45.0%
	TTACCATCACTTGTGGGAGC+AGG	+	chr1.3:14866946-14866965	None:intergenic	50.0%
!!	TTTTGGGTGGTCGTGTTCCT+TGG	-	chr1.3:14866859-14866878	MS.gene054138:CDS	50.0%
	GAAGCAAGGCCACCAGCATT+AGG	+	chr1.3:14867799-14867818	None:intergenic	55.0%
	GTGAGCTCCCCTCCTACAAA+AGG	+	chr1.3:14867645-14867664	None:intergenic	55.0%
	GCTCCTGCTCCCACAAGTGA+TGG	-	chr1.3:14866940-14866959	MS.gene054138:intron	60.0%
	TCACTTGTGGGAGCAGGAGC+AGG	+	chr1.3:14866940-14866959	None:intergenic	60.0%
	GTGGGAGCAGGAGCAGGAGC+CGG	+	chr1.3:14866934-14866953	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	14866847	14867838	14866847	ID=MS.gene054138
chr1.3	mRNA	14866847	14867838	14866847	ID=MS.gene054138.t1;Parent=MS.gene054138
chr1.3	exon	14867724	14867838	14867724	ID=MS.gene054138.t1.exon1;Parent=MS.gene054138.t1
chr1.3	CDS	14867724	14867838	14867724	ID=cds.MS.gene054138.t1;Parent=MS.gene054138.t1
chr1.3	exon	14866847	14866920	14866847	ID=MS.gene054138.t1.exon2;Parent=MS.gene054138.t1
chr1.3	CDS	14866847	14866920	14866847	ID=cds.MS.gene054138.t1;Parent=MS.gene054138.t1

Gene Sequence

>MS.gene054138

ATGGCAGTTTCATTTTGGGTGGTCGTGTTCCTTGGTCTTATTTACGCATCTTTTGCGTCAATGGCTTCTTCACAGTCTGTTGCTCCGGCTCCTGCTCCTGCTCCCACAAGTGATGGTAAGTTTTTATTAAGATTATGATTTTAACATAGGTTTTTCAAGTATTCATAAACTTCATCCTTCGATGTTGATCTCACCATCTTATATGGTTCATGGATTATAATCCTAATTGTGTTATTTTTCTTGAGAATCAAACTTGTGAAAGTTGAATGATTATGAAATTATAATATAAGTCATAAATTCTTGATTTTGATTATGAAAGATAAGATTTTACCTCATGTTGAGAAACTCTTTTTGTCTTGATAATAATATCTCAATCAAAAACTTTAAATAAGGTTTACAGTTGCAATTTAGACTACAACATCTGGTATTTGATGTCTCAGTGACCACAATGTGATTCTGGTTGAAGTCGCACCAATCATGTATAGCTGTGATTCTCTAAAATAACAAAATTTGTGATGCGTCGTCGCGATTTGAAATATTTCTCTCATTTTCTTTTGAGTTTCTTTGCCTAATAAATCTAGACTATATTGGGTTTCATGCACCGATGAGAATGGAACCATGATTAACAATTCACAAACAGTTTTGTTCAATTCAGTTTGTGAACTGTGCCACAATTTAATTATTTTTCGGTGTGACTTTTGGTTTTTTTTTTTCAACGGTCCTATTATCAGTTAATAATAACAATTTGATATAGTTCGATTCAAAAAGGTACAGTTCAACTTTCAGATATGGATGCCTTTTGTAGGAGGGGAGCTCACATTTAAATGAAACCCACTCCATAGTTATATTGTATCAAATGTTGTTGTATGGTTTCAAACCGCATGGCTAATAATTAAAATTGTTCTTAAAATTTTGCAGGAACATCAATTGACCAAGCAATAGCATATGTCCTAATGCTGGTGGCCTTGCTTCTCACTTACATCATTCATTAA

Protein sequence

>MS.gene054138.t1

MAVSFWVVVFLGLIYASFASMASSQSVAPAPAPAPTSDGTSIDQAIAYVLMLVALLLTYIIH