Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08201.t1 | XP_003624630.2 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 6.00E-18 | 99.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08201.t1 | Q9LVC0 | 54.1 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 7.0e-07 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08201.t1 | G7KZH1 | 98.3 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 4.4e-18 | 99.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049983 | MS.gene08201 | 0.815181 | 1.05E-51 | -1.69E-46 |
| MS.gene050654 | MS.gene08201 | 0.81162 | 6.39E-51 | -1.69E-46 |
| MS.gene050759 | MS.gene08201 | 0.804262 | 2.35E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene08201 | 0.802627 | 5.13E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene08201 | 0.816512 | 5.32E-52 | -1.69E-46 |
| MS.gene052342 | MS.gene08201 | 0.803332 | 3.67E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene08201 | 0.817369 | 3.42E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene08201 | 0.83192 | 1.27E-55 | -1.69E-46 |
| MS.gene056480 | MS.gene08201 | 0.808584 | 2.88E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene08201 | 0.849476 | 3.16E-60 | -1.69E-46 |
| MS.gene060932 | MS.gene08201 | 0.829393 | 5.28E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08201.t1 | MTR_7g085780 | 98.305 | 59 | 1 | 0 | 1 | 59 | 1 | 59 | 2.48e-33 | 108 |
| MS.gene08201.t1 | MTR_6g029260 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 8.81e-15 | 61.6 |
| MS.gene08201.t1 | MTR_6g029330 | 67.797 | 59 | 19 | 0 | 1 | 59 | 1 | 59 | 1.94e-13 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08201.t1 | AT5G56540 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 2.43e-14 | 60.5 |
| MS.gene08201.t1 | AT3G13520 | 54.098 | 61 | 25 | 2 | 1 | 59 | 1 | 60 | 4.02e-11 | 52.4 |
Find 8 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCATAGCACTTGCATT+TGG | 0.360344 | 7.3:-22918625 | MS.gene08201:CDS |
| GGCAGTTATGGTTGCTATGT+TGG | 0.451707 | 7.3:-22918740 | MS.gene08201:CDS |
| GTAGCATCAGAAGCTGGACT+TGG | 0.472931 | 7.3:+22918676 | None:intergenic |
| TCAGAAGCTGGACTTGGAGC+TGG | 0.503706 | 7.3:+22918682 | None:intergenic |
| AGTGTTGTAGCATCAGAAGC+TGG | 0.542379 | 7.3:+22918670 | None:intergenic |
| ATGAGAGAAGCAAAAGCGGT+TGG | 0.552907 | 7.3:+22918640 | None:intergenic |
| TGCTATGAGAGAAGCAAAAG+CGG | 0.592330 | 7.3:+22918636 | None:intergenic |
| ATGTTGGTGATAGCAACAAG+TGG | 0.753532 | 7.3:-22918724 | MS.gene08201:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAGATGAACATTTTTG+TGG | - | chr7.3:22918620-22918639 | MS.gene08201:CDS | 25.0% |
| ! | GAACATTTTTGTGGCAGTTA+TGG | - | chr7.3:22918629-22918648 | MS.gene08201:CDS | 35.0% |
| TCTCTCATAGCACTTGCATT+TGG | - | chr7.3:22918756-22918775 | MS.gene08201:CDS | 40.0% | |
| TGCTATGAGAGAAGCAAAAG+CGG | + | chr7.3:22918748-22918767 | None:intergenic | 40.0% | |
| !! | ATGTTGGTGATAGCAACAAG+TGG | - | chr7.3:22918657-22918676 | MS.gene08201:CDS | 40.0% |
| AGTGTTGTAGCATCAGAAGC+TGG | + | chr7.3:22918714-22918733 | None:intergenic | 45.0% | |
| ATGAGAGAAGCAAAAGCGGT+TGG | + | chr7.3:22918744-22918763 | None:intergenic | 45.0% | |
| GGCAGTTATGGTTGCTATGT+TGG | - | chr7.3:22918641-22918660 | MS.gene08201:CDS | 45.0% | |
| GTAGCATCAGAAGCTGGACT+TGG | + | chr7.3:22918708-22918727 | None:intergenic | 50.0% | |
| TCAGAAGCTGGACTTGGAGC+TGG | + | chr7.3:22918702-22918721 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 22918612 | 22918791 | 22918612 | ID=MS.gene08201 |
| chr7.3 | mRNA | 22918612 | 22918791 | 22918612 | ID=MS.gene08201.t1;Parent=MS.gene08201 |
| chr7.3 | exon | 22918612 | 22918791 | 22918612 | ID=MS.gene08201.t1.exon1;Parent=MS.gene08201.t1 |
| chr7.3 | CDS | 22918612 | 22918791 | 22918612 | ID=cds.MS.gene08201.t1;Parent=MS.gene08201.t1 |
| Gene Sequence |
| Protein sequence |
