AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09356

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09356.t1 MTR_5g067350 98.104 211 4 0 1 211 137 347 1.85e-153 429
MS.gene09356.t1 MTR_8g067530 74.860 179 41 2 36 211 179 356 4.33e-96 284
MS.gene09356.t1 MTR_5g030710 72.487 189 47 3 27 211 148 335 2.77e-94 278
MS.gene09356.t1 MTR_5g067350 96.324 136 5 0 1 136 137 272 5.05e-90 265
MS.gene09356.t1 MTR_2g101500 70.588 170 45 3 46 211 158 326 1.52e-82 248
MS.gene09356.t1 MTR_6g488150 69.753 162 45 2 53 211 94 254 5.13e-78 234
MS.gene09356.t1 MTR_8g103030 67.073 164 46 3 53 211 133 293 1.67e-76 232
MS.gene09356.t1 MTR_1g080860 65.854 164 51 3 52 210 89 252 1.24e-71 218
MS.gene09356.t1 MTR_7g096090 65.823 158 52 2 55 211 94 250 1.90e-70 215
MS.gene09356.t1 MTR_1g070520 59.887 177 66 4 40 211 69 245 4.31e-69 211
MS.gene09356.t1 MTR_1g093240 54.688 192 84 3 22 211 46 236 2.36e-67 207
MS.gene09356.t1 MTR_1g080860 64.286 154 50 3 52 200 89 242 8.71e-65 200
MS.gene09356.t1 MTR_1g093240 53.889 180 80 3 22 199 46 224 1.18e-60 190
MS.gene09356.t1 MTR_1g070830 56.790 162 45 5 42 200 63 202 1.11e-51 166
MS.gene09356.t1 MTR_1g040675 51.852 162 54 4 39 200 46 183 4.52e-49 158
MS.gene09356.t1 MTR_1g093240 60.630 127 48 2 55 179 78 204 5.19e-49 159
MS.gene09356.t1 MTR_1g076270 52.229 157 48 5 44 199 48 178 3.25e-48 156
MS.gene09356.t1 MTR_1g093350 52.229 157 48 5 44 199 48 178 3.25e-48 156
MS.gene09356.t1 MTR_1g040675 51.064 141 45 4 39 179 46 162 5.48e-39 132
MS.gene09356.t1 MTR_1g069495 42.935 184 70 4 59 207 81 264 1.21e-37 132
MS.gene09356.t1 MTR_4g011880 49.324 148 46 4 58 203 52 172 7.34e-37 127
MS.gene09356.t1 MTR_2g100780 40.217 184 78 6 46 202 85 263 6.49e-33 119
MS.gene09356.t1 MTR_2g102490 54.902 102 37 3 96 194 88 183 9.00e-33 117
MS.gene09356.t1 MTR_2g100780 40.571 175 74 5 46 195 85 254 1.00e-32 119
MS.gene09356.t1 MTR_8g014520 41.143 175 85 4 39 195 86 260 1.43e-32 119
MS.gene09356.t1 MTR_8g014520 41.143 175 85 4 39 195 86 260 1.70e-32 118
MS.gene09356.t1 MTR_6g488150 66.667 81 26 1 56 136 97 176 4.42e-31 112
MS.gene09356.t1 MTR_4g124300 33.987 153 71 6 50 196 56 184 3.20e-20 84.3
MS.gene09356.t1 MTR_1g085750 40.741 108 48 3 94 199 83 176 4.35e-18 78.6
MS.gene09356.t1 MTR_3g106850 31.469 143 72 5 60 196 88 210 5.83e-17 76.3
MS.gene09356.t1 MTR_5g043705 70.000 50 15 0 18 67 17 66 1.42e-16 72.0
MS.gene09356.t1 MTR_7g110790 38.384 99 50 2 94 192 68 155 2.23e-16 73.6
MS.gene09356.t1 MTR_1g109510 31.690 142 80 3 51 192 44 168 2.76e-16 73.9
MS.gene09356.t1 MTR_4g115075 31.343 134 71 4 61 193 93 206 2.69e-15 71.6
MS.gene09356.t1 MTR_5g057080 76.923 39 9 0 17 55 38 76 1.19e-13 64.7
MS.gene09356.t1 MTR_1g085750 40.909 88 37 2 94 179 83 157 2.31e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09356.t1 AT2G22670 70.238 168 45 3 47 210 152 318 9.32e-83 249
MS.gene09356.t1 AT2G22670 70.238 168 45 3 47 210 152 318 9.32e-83 249
MS.gene09356.t1 AT2G22670 70.238 168 45 3 47 210 169 335 1.26e-82 249
MS.gene09356.t1 AT5G65670 72.152 158 40 2 56 210 179 335 2.57e-82 248
MS.gene09356.t1 AT5G65670 71.250 160 40 3 56 210 179 337 1.78e-80 244
MS.gene09356.t1 AT2G22670 61.165 206 60 6 21 210 115 316 1.95e-79 240
MS.gene09356.t1 AT2G22670 61.165 206 60 6 21 210 115 316 1.95e-79 240
MS.gene09356.t1 AT4G29080 62.500 184 58 3 39 211 122 305 4.02e-77 234
MS.gene09356.t1 AT4G14550 59.877 162 64 1 51 211 67 228 2.75e-67 206
MS.gene09356.t1 AT1G04250 57.647 170 58 3 46 210 68 228 2.79e-65 201
MS.gene09356.t1 AT3G04730 58.721 172 56 3 55 211 65 236 8.02e-65 200
MS.gene09356.t1 AT3G23050 58.788 165 59 3 51 206 73 237 1.70e-61 192
MS.gene09356.t1 AT4G14550 58.940 151 61 1 51 200 67 217 8.12e-61 190
MS.gene09356.t1 AT1G04240 53.548 155 54 4 46 200 50 186 5.22e-51 164
MS.gene09356.t1 AT1G04240 53.896 154 53 4 46 199 150 285 5.42e-50 164
MS.gene09356.t1 AT4G14550 58.140 129 53 1 51 178 67 195 1.89e-48 157
MS.gene09356.t1 AT5G43700 51.299 154 55 3 47 200 49 182 1.14e-47 155
MS.gene09356.t1 AT3G23050 56.522 138 51 3 51 179 73 210 2.90e-44 147
MS.gene09356.t1 AT3G23030 48.980 147 46 2 53 199 53 170 9.30e-43 142
MS.gene09356.t1 AT3G15540 49.359 156 54 5 54 206 64 197 5.86e-42 140
MS.gene09356.t1 AT3G23030 48.980 147 46 2 53 199 140 257 6.98e-42 142
MS.gene09356.t1 AT2G33310 44.624 186 67 6 46 205 62 237 4.16e-40 137
MS.gene09356.t1 AT1G52830 45.638 149 56 4 51 199 61 184 6.76e-40 135
MS.gene09356.t1 AT2G33310 44.624 186 66 7 46 205 62 236 1.65e-38 133
MS.gene09356.t1 AT2G33310 44.624 186 66 7 46 205 62 236 1.65e-38 133
MS.gene09356.t1 AT4G28640 46.914 162 66 4 56 200 84 242 3.66e-38 132
MS.gene09356.t1 AT4G28640 46.875 160 65 4 56 198 84 240 6.64e-38 132
MS.gene09356.t1 AT1G04550 42.564 195 82 5 39 211 50 236 8.86e-38 131
MS.gene09356.t1 AT1G15580 41.111 180 68 5 22 194 11 159 3.01e-37 127
MS.gene09356.t1 AT4G28640 46.875 160 65 4 56 198 84 240 6.58e-37 130
MS.gene09356.t1 AT4G14560 49.655 145 46 3 52 196 47 164 4.31e-36 125
MS.gene09356.t1 AT1G04100 41.071 168 66 5 62 199 95 259 4.02e-32 117
MS.gene09356.t1 AT3G16500 39.205 176 77 5 49 195 78 252 5.63e-32 117
MS.gene09356.t1 AT3G16500 39.205 176 77 5 49 195 78 252 5.86e-32 117
MS.gene09356.t1 AT1G51950 37.647 170 85 4 46 195 82 250 7.74e-31 114
MS.gene09356.t1 AT5G25890 36.686 169 87 3 34 202 22 170 1.01e-28 106
MS.gene09356.t1 AT4G14550 45.238 126 64 2 14 135 27 151 6.29e-27 102
MS.gene09356.t1 AT3G17600 39.375 160 68 8 39 192 18 154 1.94e-25 97.4
MS.gene09356.t1 AT3G23050 56.180 89 31 2 55 135 77 165 8.42e-23 90.9
MS.gene09356.t1 AT2G46990 40.385 104 50 1 91 194 81 172 1.44e-20 85.1
MS.gene09356.t1 AT3G62100 39.604 101 49 1 93 193 81 169 2.61e-20 84.3
MS.gene09356.t1 AT3G62100 39.080 87 41 1 93 179 81 155 8.60e-15 69.3
MS.gene09356.t1 AT2G46990 39.080 87 41 1 93 179 83 157 1.52e-14 68.6
MS.gene09356.t1 AT1G15050 37.255 102 51 1 96 197 62 150 1.76e-14 68.6
MS.gene09356.t1 AT1G15050 37.255 102 51 1 96 197 94 182 4.76e-14 68.2
MS.gene09356.t1 AT2G01200 34.906 106 56 1 92 197 96 188 2.76e-13 66.2
MS.gene09356.t1 AT2G01200 34.906 106 56 1 92 197 96 188 2.76e-13 66.2
MS.gene09356.t1 AT4G32280 32.653 98 49 2 95 192 118 198 1.03e-12 64.7
MS.gene09356.t1 AT4G32280 24.848 165 76 4 59 192 93 240 1.78e-12 64.7

Find 48 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAGGTTGATGGAAAAT+TGG 0.178401 5.3:+53687227 MS.gene09356:CDS
GGCAACATTCTCAAGCATTT+TGG 0.180467 5.3:-53686618 None:intergenic
GAAAGCAAACCAAGTCTTAA+TGG 0.318890 5.3:+53686710 MS.gene09356:CDS
TGCTTGTGGGTGATGTTCCC+TGG 0.320651 5.3:+53687601 MS.gene09356:CDS
AGCTCTGATGCCATTGGTTT+AGG 0.336962 5.3:+53688697 MS.gene09356:CDS
CTTAATGGTTCTGCAACTAA+TGG 0.371208 5.3:+53686725 MS.gene09356:CDS
GAAGAGGTTGATGGAAAATT+GGG 0.409009 5.3:+53687228 MS.gene09356:CDS
TGTGGCAGGGCACAGGTTGT+TGG 0.411315 5.3:+53687147 MS.gene09356:intron
AGAATATGTTATTACTTATG+AGG 0.411394 5.3:+53687560 MS.gene09356:CDS
TTCATTGTTCTTTGAAGCAG+TGG 0.422775 5.3:-53687208 None:intergenic
AAAATGCTTGAGAATGTTGC+CGG 0.428584 5.3:+53686620 MS.gene09356:CDS
AAGCTGAGGGATCTGCTTCA+TGG 0.432732 5.3:+53687534 MS.gene09356:CDS
GGCCTCCAATAAGATCGTTT+AGG 0.457342 5.3:+53687172 MS.gene09356:CDS
AAGAACAATGAAGAGGTTGA+TGG 0.459868 5.3:+53687219 MS.gene09356:CDS
ATTGGTCTTGAGAGGCCTAA+TGG 0.460399 5.3:+53686680 MS.gene09356:CDS
CTTAACTGTTGCTATTTCAT+CGG 0.466939 5.3:-53686657 None:intergenic
GCAGGGCACAGGTTGTTGGT+TGG 0.475629 5.3:+53687151 MS.gene09356:intron
GAAATAGCAACAGTTAAGAT+TGG 0.476375 5.3:+53686662 MS.gene09356:CDS
TTGCTTTCACCGACACCATT+AGG 0.485059 5.3:-53686695 None:intergenic
TTCCTAAACGATCTTATTGG+AGG 0.485580 5.3:-53687174 None:intergenic
GTTGATGGAAAATTGGGATC+AGG 0.490761 5.3:+53687234 MS.gene09356:CDS
ATTACTTATGAGGATAAAGA+TGG 0.497598 5.3:+53687570 MS.gene09356:CDS
GCTTGTGGGTGATGTTCCCT+GGG 0.507108 5.3:+53687602 MS.gene09356:CDS
ATGAGGATAAAGATGGTGAT+TGG 0.518002 5.3:+53687577 MS.gene09356:CDS
ATGAAGAGCTCTGATGCCAT+TGG 0.522246 5.3:+53688691 MS.gene09356:CDS
ATGTTCAGCTGTTTCACCAT+AGG 0.526626 5.3:+53687363 MS.gene09356:CDS
AATCTTGTGTGGCAGGGCAC+AGG 0.532182 5.3:+53687140 MS.gene09356:intron
CACCCTACTTACTTGCTAGC+AGG 0.532961 5.3:-53686761 None:intergenic
GATGGTGATTGGATGCTTGT+GGG 0.535428 5.3:+53687588 MS.gene09356:CDS
TTTGTGAAGGTCAGCATGGA+TGG 0.538161 5.3:+53687267 MS.gene09356:CDS
ATCGGCTTTCACTTTCTGCC+CGG 0.540054 5.3:-53686639 None:intergenic
GGATGTTTATTGATACATGC+AGG 0.544403 5.3:+53688656 MS.gene09356:intron
TCAGGTCAATGTGAATCTCA+TGG 0.548741 5.3:+53687489 MS.gene09356:intron
CAGTTAAGATTGGTCTTGAG+AGG 0.564900 5.3:+53686672 MS.gene09356:CDS
GTTGCAGAACCATTAAGACT+TGG 0.566322 5.3:-53686719 None:intergenic
ATGAAGCAGATCCCTCAGCT+TGG 0.567500 5.3:-53687532 None:intergenic
AGATGGTGATTGGATGCTTG+TGG 0.576179 5.3:+53687587 MS.gene09356:CDS
AAATGCTTGAGAATGTTGCC+GGG 0.605417 5.3:+53686621 MS.gene09356:CDS
GGAATACTAACCTAAACCAA+TGG 0.612277 5.3:-53688707 None:intergenic
TGCTTCAAAGAACAATGAAG+AGG 0.621204 5.3:+53687212 MS.gene09356:CDS
AGTATATAAACTTACTCCCA+GGG 0.621275 5.3:-53687618 None:intergenic
TGCTGAATGAAACCAAGCTG+AGG 0.622855 5.3:+53687520 MS.gene09356:CDS
GCTGAATGAAACCAAGCTGA+GGG 0.626284 5.3:+53687521 MS.gene09356:CDS
GATGGAAAATTGGGATCAGG+AGG 0.633939 5.3:+53687237 MS.gene09356:CDS
CTTGAGAGGCCTAATGGTGT+CGG 0.641657 5.3:+53686686 MS.gene09356:CDS
AGGAGGTGCTGTGTTTGTGA+AGG 0.651647 5.3:+53687254 MS.gene09356:CDS
TGTGTTTGTGAAGGTCAGCA+TGG 0.667221 5.3:+53687263 MS.gene09356:CDS
TGTTTATTGATACATGCAGG+AGG 0.676885 5.3:+53688659 MS.gene09356:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATCAAAAATAGAAAT+TGG - chr5.3:53688858-53688877 None:intergenic 10.0%
!!! CATATTTTATTATCTTATCA+CGG + chr5.3:53687868-53687887 MS.gene09356:intron 15.0%
!! AAATCACAAAAATTGAAATG+CGG - chr5.3:53686954-53686973 None:intergenic 20.0%
!! AATAAAAGCTTGAATGATAA+TGG + chr5.3:53688136-53688155 MS.gene09356:intron 20.0%
!! AATGTGACTTCAAATTAAAA+TGG + chr5.3:53688217-53688236 MS.gene09356:intron 20.0%
!! ACTAAATGAATATGTTACAT+GGG + chr5.3:53688400-53688419 MS.gene09356:intron 20.0%
!! AGAATATGTTATTACTTATG+AGG + chr5.3:53687560-53687579 MS.gene09356:CDS 20.0%
!! ATGTAAGGTATATTAATAGA+AGG + chr5.3:53688069-53688088 MS.gene09356:intron 20.0%
!! TTCTATTAATATACCTTACA+TGG - chr5.3:53688070-53688089 None:intergenic 20.0%
!!! AAAGTAAATTAAGTTGAAGT+AGG - chr5.3:53688834-53688853 None:intergenic 20.0%
!!! TTGATACGATTTTATTGAAA+GGG + chr5.3:53688504-53688523 MS.gene09356:intron 20.0%
!!! TTGTGATTTTAGTTTGAAAT+AGG + chr5.3:53688040-53688059 MS.gene09356:intron 20.0%
!!! TTTGATACGATTTTATTGAA+AGG + chr5.3:53688503-53688522 MS.gene09356:intron 20.0%
! AAATCAAAGTGAAGTAAACA+AGG - chr5.3:53688581-53688600 None:intergenic 25.0%
! ATTACTTATGAGGATAAAGA+TGG + chr5.3:53687570-53687589 MS.gene09356:CDS 25.0%
! CACTAAATGAATATGTTACA+TGG + chr5.3:53688399-53688418 MS.gene09356:intron 25.0%
! CTAAATGAATATGTTACATG+GGG + chr5.3:53688401-53688420 MS.gene09356:intron 25.0%
! CTTAACAATGAAAATGAACT+CGG + chr5.3:53687103-53687122 MS.gene09356:intron 25.0%
! GATTGATTGTGTTAAATGAA+TGG - chr5.3:53688731-53688750 None:intergenic 25.0%
!! CACATAATTTGTACTTGAAT+TGG - chr5.3:53688913-53688932 None:intergenic 25.0%
!! CTTTTATTAAAAGCTTGGTT+TGG + chr5.3:53687080-53687099 MS.gene09356:intron 25.0%
!! TTTGAATTGGCTTAAATTGT+TGG - chr5.3:53688244-53688263 None:intergenic 25.0%
!! TTTTTCCTAAACGATCTTAT+TGG - chr5.3:53687180-53687199 None:intergenic 25.0%
!!! AAGTCTACTTTTCTCAAATA+CGG - chr5.3:53687297-53687316 None:intergenic 25.0%
!!! TGATTTTAGTTTGAAATAGG+TGG + chr5.3:53688043-53688062 MS.gene09356:intron 25.0%
AAAAGTAAAAGCAGGAACTA+AGG + chr5.3:53688968-53688987 MS.gene09356:CDS 30.0%
AAAGTAAAAGCAGGAACTAA+GGG + chr5.3:53688969-53688988 MS.gene09356:CDS 30.0%
AAATCGTATCAAATTCGCTA+TGG - chr5.3:53688496-53688515 None:intergenic 30.0%
AAGTATATAAACTTACTCCC+AGG - chr5.3:53687622-53687641 None:intergenic 30.0%
AATAGTCTTTCAACGATTTG+AGG + chr5.3:53687421-53687440 MS.gene09356:intron 30.0%
AATATATCTCAACTCTTTGC+AGG + chr5.3:53688635-53688654 MS.gene09356:intron 30.0%
AGTATATAAACTTACTCCCA+GGG - chr5.3:53687621-53687640 None:intergenic 30.0%
AGTTGAGATATATTAGGTGT+TGG - chr5.3:53688629-53688648 None:intergenic 30.0%
ATACATACACATTGTTGTGT+CGG + chr5.3:53687761-53687780 MS.gene09356:intron 30.0%
ATGAGGAAAATTCTTAGAGA+GGG + chr5.3:53688527-53688546 MS.gene09356:intron 30.0%
ATGTTTGGAAATTGGAATCA+TGG - chr5.3:53688370-53688389 None:intergenic 30.0%
CAAGTACAAATTATGTGTTG+TGG + chr5.3:53688916-53688935 MS.gene09356:intron 30.0%
CAGTTGAAAAAAGTAAAAGC+AGG + chr5.3:53688960-53688979 MS.gene09356:CDS 30.0%
GAAATAGCAACAGTTAAGAT+TGG + chr5.3:53686662-53686681 MS.gene09356:CDS 30.0%
GCAAAGAGTTGAGATATATT+AGG - chr5.3:53688635-53688654 None:intergenic 30.0%
GTCTTCAATAATGCCAAAAA+TGG - chr5.3:53688105-53688124 None:intergenic 30.0%
TGAAACGCAAAATTGAAAAC+AGG - chr5.3:53688444-53688463 None:intergenic 30.0%
TGGGAGTAAGTTTATATACT+TGG + chr5.3:53687621-53687640 MS.gene09356:intron 30.0%
! GCGAACTTTTATTAAAAGCT+TGG + chr5.3:53687075-53687094 MS.gene09356:intron 30.0%
! GTCCTAAATTTAAAACCTTG+CGG - chr5.3:53686810-53686829 None:intergenic 30.0%
!! CTTAACTGTTGCTATTTCAT+CGG - chr5.3:53686660-53686679 None:intergenic 30.0%
!!! TACCGCAAGGTTTTAAATTT+AGG + chr5.3:53686805-53686824 MS.gene09356:intron 30.0%
!!! TTACTTTTTTCAACTGCTCT+GGG - chr5.3:53688956-53688975 None:intergenic 30.0%
!!! TTTACTTTTTTCAACTGCTC+TGG - chr5.3:53688957-53688976 None:intergenic 30.0%
AAAATGCTTGAGAATGTTGC+CGG + chr5.3:53686620-53686639 MS.gene09356:CDS 35.0%
AAGAACAATGAAGAGGTTGA+TGG + chr5.3:53687219-53687238 MS.gene09356:CDS 35.0%
ATCGCAGTTACTTACAAATG+CGG + chr5.3:53686879-53686898 MS.gene09356:intron 35.0%
ATGACTGCATGTTTGGAAAT+TGG - chr5.3:53688378-53688397 None:intergenic 35.0%
ATGAGGATAAAGATGGTGAT+TGG + chr5.3:53687577-53687596 MS.gene09356:CDS 35.0%
CACCATTATTTCGTCTAACT+CGG - chr5.3:53688289-53688308 None:intergenic 35.0%
CTTGAATCACGATTGTATCA+CGG + chr5.3:53687006-53687025 MS.gene09356:intron 35.0%
GAAAGCAAACCAAGTCTTAA+TGG + chr5.3:53686710-53686729 MS.gene09356:CDS 35.0%
GATGAGGAAAATTCTTAGAG+AGG + chr5.3:53688526-53688545 MS.gene09356:intron 35.0%
GATGATTGATGAATCTTGTG+TGG + chr5.3:53687129-53687148 MS.gene09356:intron 35.0%
GCAGTACAAATAGTACAGTA+TGG - chr5.3:53688169-53688188 None:intergenic 35.0%
GCTTATGTTGATGTGATTTC+AGG + chr5.3:53687471-53687490 MS.gene09356:intron 35.0%
GGAATACTAACCTAAACCAA+TGG - chr5.3:53688710-53688729 None:intergenic 35.0%
GGATGTTTATTGATACATGC+AGG + chr5.3:53688656-53688675 MS.gene09356:intron 35.0%
TGCTTCAAAGAACAATGAAG+AGG + chr5.3:53687212-53687231 MS.gene09356:CDS 35.0%
TGTTTATTGATACATGCAGG+AGG + chr5.3:53688659-53688678 MS.gene09356:CDS 35.0%
TTAGTGTATGACTGCATGTT+TGG - chr5.3:53688385-53688404 None:intergenic 35.0%
TTCCTAAACGATCTTATTGG+AGG - chr5.3:53687177-53687196 None:intergenic 35.0%
TTTGTAAGTAACTGCGATTC+AGG - chr5.3:53686877-53686896 None:intergenic 35.0%
! CGATTTTATTGAAAGGGATG+AGG + chr5.3:53688510-53688529 MS.gene09356:intron 35.0%
! CTTAATGGTTCTGCAACTAA+TGG + chr5.3:53686725-53686744 MS.gene09356:CDS 35.0%
!! GAAGAGGTTGATGGAAAATT+GGG + chr5.3:53687228-53687247 MS.gene09356:CDS 35.0%
!! TGAAGAGGTTGATGGAAAAT+TGG + chr5.3:53687227-53687246 MS.gene09356:CDS 35.0%
!! TTCATTGTTCTTTGAAGCAG+TGG - chr5.3:53687211-53687230 None:intergenic 35.0%
!! TTTGAGGCAATTTTTGTGTC+TGG + chr5.3:53687437-53687456 MS.gene09356:intron 35.0%
!!! ACAAGCGCTAAGTTTTGAAT+TGG - chr5.3:53688257-53688276 None:intergenic 35.0%
!!! AGGTAAGAAACTGCCATTTT+TGG + chr5.3:53688089-53688108 MS.gene09356:intron 35.0%
AAATGCTTGAGAATGTTGCC+GGG + chr5.3:53686621-53686640 MS.gene09356:CDS 40.0%
AACCTATTCCTACCTACCTA+TGG - chr5.3:53687382-53687401 None:intergenic 40.0%
AGCCGAGTTAGACGAAATAA+TGG + chr5.3:53688284-53688303 MS.gene09356:intron 40.0%
ATTGATGAATCTTGTGTGGC+AGG + chr5.3:53687133-53687152 MS.gene09356:intron 40.0%
CGATTAATGAAGCACAGACA+CGG + chr5.3:53687656-53687675 MS.gene09356:intron 40.0%
CTGTACTATTTGTACTGCGT+TGG + chr5.3:53688171-53688190 MS.gene09356:intron 40.0%
GAATATGTTACATGGGGCTT+AGG + chr5.3:53688407-53688426 MS.gene09356:intron 40.0%
GTTGCAGAACCATTAAGACT+TGG - chr5.3:53686722-53686741 None:intergenic 40.0%
TCAGGTCAATGTGAATCTCA+TGG + chr5.3:53687489-53687508 MS.gene09356:intron 40.0%
TGAAATAGGTGGTCCATGTA+AGG + chr5.3:53688054-53688073 MS.gene09356:intron 40.0%
TTGATGAATCTTGTGTGGCA+GGG + chr5.3:53687134-53687153 MS.gene09356:intron 40.0%
! ATGTTCAGCTGTTTCACCAT+AGG + chr5.3:53687363-53687382 MS.gene09356:CDS 40.0%
! GACATCGTGGTTTTTGATGT+TGG - chr5.3:53687037-53687056 None:intergenic 40.0%
! GGCAACATTCTCAAGCATTT+TGG - chr5.3:53686621-53686640 None:intergenic 40.0%
! GTTGATGGAAAATTGGGATC+AGG + chr5.3:53687234-53687253 MS.gene09356:CDS 40.0%
!! AGGTGTTGGAATTAGTTTGC+AGG - chr5.3:53688615-53688634 None:intergenic 40.0%
!! CAGTTAAGATTGGTCTTGAG+AGG + chr5.3:53686672-53686691 MS.gene09356:CDS 40.0%
!! GGTGTTGGAATTAGTTTGCA+GGG - chr5.3:53688614-53688633 None:intergenic 40.0%
!!! TTCTCAAGCATTTTGGAGCT+AGG - chr5.3:53686614-53686633 None:intergenic 40.0%
AAAAGTTCGCAACCGAAGTC+TGG - chr5.3:53687065-53687084 None:intergenic 45.0%
AAAGTTCGCAACCGAAGTCT+GGG - chr5.3:53687064-53687083 None:intergenic 45.0%
AGATGGTGATTGGATGCTTG+TGG + chr5.3:53687587-53687606 MS.gene09356:CDS 45.0%
CACCATAGGTAGGTAGGAAT+AGG + chr5.3:53687377-53687396 MS.gene09356:intron 45.0%
CATCAAAAACCACGATGTCG+CGG + chr5.3:53687038-53687057 MS.gene09356:intron 45.0%
CTCAGATTGCGGTCAAATAG+TGG + chr5.3:53686928-53686947 MS.gene09356:intron 45.0%
CTCCTGCTAGCAAGTAAGTA+GGG + chr5.3:53686759-53686778 MS.gene09356:intron 45.0%
CTGTTTCACCATAGGTAGGT+AGG + chr5.3:53687371-53687390 MS.gene09356:intron 45.0%
GATGGAAAATTGGGATCAGG+AGG + chr5.3:53687237-53687256 MS.gene09356:CDS 45.0%
GATGGTGATTGGATGCTTGT+GGG + chr5.3:53687588-53687607 MS.gene09356:CDS 45.0%
GCTGAATGAAACCAAGCTGA+GGG + chr5.3:53687521-53687540 MS.gene09356:CDS 45.0%
GGCCTCCAATAAGATCGTTT+AGG + chr5.3:53687172-53687191 MS.gene09356:CDS 45.0%
GTAAGTAGGGTGGTGTGAAT+TGG + chr5.3:53686772-53686791 MS.gene09356:intron 45.0%
TGCTGAATGAAACCAAGCTG+AGG + chr5.3:53687520-53687539 MS.gene09356:CDS 45.0%
TGGCATTAGAGTTTACCGCA+AGG + chr5.3:53686792-53686811 MS.gene09356:intron 45.0%
TGTGTTTGTGAAGGTCAGCA+TGG + chr5.3:53687263-53687282 MS.gene09356:CDS 45.0%
TTGCTTTCACCGACACCATT+AGG - chr5.3:53686698-53686717 None:intergenic 45.0%
TTTGTGAAGGTCAGCATGGA+TGG + chr5.3:53687267-53687286 MS.gene09356:CDS 45.0%
! ATGAAGAGCTCTGATGCCAT+TGG + chr5.3:53688691-53688710 MS.gene09356:CDS 45.0%
! TCAGCTGTTTCACCATAGGT+AGG + chr5.3:53687367-53687386 MS.gene09356:intron 45.0%
!! AGCTCTGATGCCATTGGTTT+AGG + chr5.3:53688697-53688716 MS.gene09356:CDS 45.0%
!! ATTGGTCTTGAGAGGCCTAA+TGG + chr5.3:53686680-53686699 MS.gene09356:CDS 45.0%
AAGCTGAGGGATCTGCTTCA+TGG + chr5.3:53687534-53687553 MS.gene09356:CDS 50.0%
ATGAAGCAGATCCCTCAGCT+TGG - chr5.3:53687535-53687554 None:intergenic 50.0%
CAATCTGAGCTGCGACATCA+TGG - chr5.3:53686917-53686936 None:intergenic 50.0%
CTGCTAGCAAGTAAGTAGGG+TGG + chr5.3:53686762-53686781 MS.gene09356:intron 50.0%
CTTGAGAGGCCTAATGGTGT+CGG + chr5.3:53686686-53686705 MS.gene09356:CDS 50.0%
GCTCCTGCTAGCAAGTAAGT+AGG + chr5.3:53686758-53686777 MS.gene09356:intron 50.0%
TGATGTCGCAGCTCAGATTG+CGG + chr5.3:53686917-53686936 MS.gene09356:intron 50.0%
! ATCGGCTTTCACTTTCTGCC+CGG - chr5.3:53686642-53686661 None:intergenic 50.0%
! CACCCTACTTACTTGCTAGC+AGG - chr5.3:53686764-53686783 None:intergenic 50.0%
!! AGGAGGTGCTGTGTTTGTGA+AGG + chr5.3:53687254-53687273 MS.gene09356:CDS 50.0%
AATCTTGTGTGGCAGGGCAC+AGG + chr5.3:53687140-53687159 MS.gene09356:intron 55.0%
GCTTGTGGGTGATGTTCCCT+GGG + chr5.3:53687602-53687621 MS.gene09356:CDS 55.0%
TGCTTGTGGGTGATGTTCCC+TGG + chr5.3:53687601-53687620 MS.gene09356:CDS 55.0%
!! CTGCTCTGGGAGCTGCAAAA+TGG - chr5.3:53688943-53688962 None:intergenic 55.0%
GCAGGGCACAGGTTGTTGGT+TGG + chr5.3:53687151-53687170 MS.gene09356:intron 60.0%
TGTGGCAGGGCACAGGTTGT+TGG + chr5.3:53687147-53687166 MS.gene09356:intron 60.0%
AAGTCTGGGCCGCGACATCG+TGG - chr5.3:53687050-53687069 None:intergenic 65.0%
CGATGTCGCGGCCCAGACTT+CGG + chr5.3:53687050-53687069 MS.gene09356:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 53686599 53688988 53686599 ID=MS.gene09356
chr5.3 mRNA 53686599 53688988 53686599 ID=MS.gene09356.t1;Parent=MS.gene09356
chr5.3 exon 53686599 53686771 53686599 ID=MS.gene09356.t1.exon1;Parent=MS.gene09356.t1
chr5.3 CDS 53686599 53686771 53686599 ID=cds.MS.gene09356.t1;Parent=MS.gene09356.t1
chr5.3 exon 53687155 53687384 53687155 ID=MS.gene09356.t1.exon2;Parent=MS.gene09356.t1
chr5.3 CDS 53687155 53687384 53687155 ID=cds.MS.gene09356.t1;Parent=MS.gene09356.t1
chr5.3 exon 53687493 53687625 53687493 ID=MS.gene09356.t1.exon3;Parent=MS.gene09356.t1
chr5.3 CDS 53687493 53687625 53687493 ID=cds.MS.gene09356.t1;Parent=MS.gene09356.t1
chr5.3 exon 53688657 53688718 53688657 ID=MS.gene09356.t1.exon4;Parent=MS.gene09356.t1
chr5.3 CDS 53688657 53688718 53688657 ID=cds.MS.gene09356.t1;Parent=MS.gene09356.t1
chr5.3 exon 53688951 53688988 53688951 ID=MS.gene09356.t1.exon5;Parent=MS.gene09356.t1
chr5.3 CDS 53688951 53688988 53688951 ID=cds.MS.gene09356.t1;Parent=MS.gene09356.t1
Gene Sequence

>MS.gene09356

GGGAAATTGAAGCCTAGCTCCAAAATGCTTGAGAATGTTGCCGGGCAGAAAGTGAAAGCCGATGAAATAGCAACAGTTAAGATTGGTCTTGAGAGGCCTAATGGTGTCGGTGAAAGCAAACCAAGTCTTAATGGTTCTGCAACTAATGGAAATAGCACTGCTCCTGCTAGCAAGTAAGTAGGGTGGTGTGAATTGGCATTAGAGTTTACCGCAAGGTTTTAAATTTAGGACATGTTACGTTTTTTGATACTGCGAATCAAATGCTGTTGATGCAGCCTGAATCGCAGTTACTTACAAATGCGGCGACATCAAAAGCCATGATGTCGCAGCTCAGATTGCGGTCAAATAGTGGCCGCATTTCAATTTTTGTGATTTTTGAGAGTCATAGTCAAATGCAGCTGATGCAGCTTGAATCACGATTGTATCACGGTCATGCCAACATCAAAAACCACGATGTCGCGGCCCAGACTTCGGTTGCGAACTTTTATTAAAAGCTTGGTTTGGCTTAACAATGAAAATGAACTCGGAATGATGATTGATGAATCTTGTGTGGCAGGGCACAGGTTGTTGGTTGGCCTCCAATAAGATCGTTTAGGAAAAATTCATTGACCACTGCTTCAAAGAACAATGAAGAGGTTGATGGAAAATTGGGATCAGGAGGTGCTGTGTTTGTGAAGGTCAGCATGGATGGTGCTCCGTATTTGAGAAAAGTAGACTTGAAGAATTACACTGCATATTCACAACTATCTTCTTCTCTTGAGAAGATGTTCAGCTGTTTCACCATAGGTAGGTAGGAATAGGTTCATTTTTGAATAAAAGAAAAATAGTCTTTCAACGATTTGAGGCAATTTTTGTGTCTGGATCAAGTTACTGCTTATGTTGATGTGATTTCAGGTCAATGTGAATCTCATGGAAATCAGATGCTGAATGAAACCAAGCTGAGGGATCTGCTTCATGGTTCAGAATATGTTATTACTTATGAGGATAAAGATGGTGATTGGATGCTTGTGGGTGATGTTCCCTGGGAGTAAGTTTATATACTTGGTTTTCTTTCACGCGATTAATGAAGCACAGACACGGAAATGACATTGACATCGTCGATATTGTAGTATTTTTAGAAAATAGAATACATACACATCATCAACATTCTACGCAGATAAGTATACATACACATTGTTGTGTCGGACACTAAACACACATTCAATCTACATAAGTATACATACACATCATCAACATTCTACGCAGATAAGTTTTATACAAACGTTTAATCATATTTTATTATCTTATCACGGTGTAAACACATATTTAAACATAATATTGTGATATTAGAAGATAATGAAATAGTTAAACTTATGTGTAAAATTTATTTATACAGTCTGATTATGCTGATAAAAATAATGAGTTCTTATAAAATAATTAAAGAAATACAAATTTTGCAGCTTTGTGATTTTAGTTTGAAATAGGTGGTCCATGTAAGGTATATTAATAGAAGGTAAGAAACTGCCATTTTTGGCATTATTGAAGACTGTACAAAAGCAATAAAAGCTTGAATGATAATGGATATAAACCATACTGTACTATTTGTACTGCGTTGGTGACATGTGAGTATAATCAGAACAATGTGACTTCAAATTAAAATGGACCAACAATTTAAGCCAATTCAAAACTTAGCGCTTGTTTAGATTAGCCGAGTTAGACGAAATAATGGTGTAGCACAATAATTTTGACATAAGTTACACTTTAGAGCTTAAACAAAATAGCAGTGCTACCATGATTCCAATTTCCAAACATGCAGTCATACACTAAATGAATATGTTACATGGGGCTTAGGATTTTGATTATCCTGTTTTCAATTTTGCGTTTCATAGTATTTATTGCTTATTTCGTAAATAATCCATAGCGAATTTGATACGATTTTATTGAAAGGGATGAGGAAAATTCTTAGAGAGGGTCTATGTTTATAAACAAAGTTTTACTAACCTTGTTTACTTCACTTTGATTTTATATCTGCACCCTGCAAACTAATTCCAACACCTAATATATCTCAACTCTTTGCAGGATGTTTATTGATACATGCAGGAGGCTAAGAATCATGAAGAGCTCTGATGCCATTGGTTTAGGTTAGTATTCCATTCATTTAACACAATCAATCTTAAAAATTGTATTGCATATATATACTAAATGATCTTAAAAAAAAGAGATATTTGTTCACTTATCACAAAATAACAGAATCCTACTTCAACTTAATTTACTTTGCCAATTTCTATTTTTGATAAAAATTTTATATATATTATACTATATGTTAGCATTTCCAATTCAAGTACAAATTATGTGTTGTGGTCCATTTTGCAGCTCCCAGAGCAGTTGAAAAAAGTAAAAGCAGGAACTAA
Protein sequence

>MS.gene09356.t1

GKLKPSSKMLENVAGQKVKADEIATVKIGLERPNGVGESKPSLNGSATNGNSTAPASKAQVVGWPPIRSFRKNSLTTASKNNEEVDGKLGSGGAVFVKVSMDGAPYLRKVDLKNYTAYSQLSSSLEKMFSCFTIGQCESHGNQMLNETKLRDLLHGSEYVITYEDKDGDWMLVGDVPWEMFIDTCRRLRIMKSSDAIGLAPRAVEKSKSRN