Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09451.t1 | KEH32008.1 | 71.8 | 255 | 61 | 3 | 7 | 259 | 134 | 379 | 8.40E-88 | 333.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09451.t1 | P42730 | 70.5 | 254 | 64 | 4 | 8 | 259 | 135 | 379 | 6.5e-89 | 328.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09451.t1 | A0A072USG1 | 71.8 | 255 | 61 | 3 | 7 | 259 | 134 | 379 | 6.0e-88 | 333.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049701 | MS.gene09451 | 0.806252 | 9.00E-50 | -1.69E-46 |
| MS.gene051710 | MS.gene09451 | 0.841814 | 3.78E-58 | -1.69E-46 |
| MS.gene052456 | MS.gene09451 | 0.825828 | 3.79E-54 | -1.69E-46 |
| MS.gene054801 | MS.gene09451 | 0.841285 | 5.21E-58 | -1.69E-46 |
| MS.gene055058 | MS.gene09451 | 0.817731 | 2.83E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene09451 | 0.882165 | 1.31E-70 | -1.69E-46 |
| MS.gene059276 | MS.gene09451 | 0.822327 | 2.51E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09451.t1 | MTR_4g109450 | 72.157 | 255 | 60 | 4 | 7 | 259 | 134 | 379 | 1.98e-112 | 342 |
| MS.gene09451.t1 | MTR_4g109450 | 72.157 | 255 | 60 | 4 | 7 | 259 | 134 | 379 | 4.74e-112 | 343 |
| MS.gene09451.t1 | MTR_7g028970 | 66.535 | 254 | 74 | 4 | 8 | 259 | 135 | 379 | 5.52e-104 | 322 |
| MS.gene09451.t1 | MTR_6g013660 | 57.642 | 229 | 84 | 5 | 34 | 258 | 236 | 455 | 4.59e-75 | 246 |
| MS.gene09451.t1 | MTR_7g012820 | 57.205 | 229 | 85 | 5 | 34 | 258 | 169 | 388 | 5.67e-75 | 244 |
| MS.gene09451.t1 | MTR_7g012820 | 57.205 | 229 | 85 | 5 | 34 | 258 | 240 | 459 | 1.14e-74 | 244 |
| MS.gene09451.t1 | MTR_3g106160 | 56.769 | 229 | 86 | 5 | 34 | 258 | 248 | 467 | 1.88e-74 | 244 |
| MS.gene09451.t1 | MTR_8g100040 | 48.548 | 241 | 110 | 6 | 22 | 258 | 244 | 474 | 1.50e-63 | 214 |
| MS.gene09451.t1 | MTR_3g102830 | 48.133 | 241 | 111 | 6 | 22 | 258 | 245 | 475 | 1.61e-62 | 211 |
| MS.gene09451.t1 | MTR_3g498725 | 48.319 | 238 | 109 | 6 | 25 | 258 | 243 | 470 | 8.30e-62 | 209 |
| MS.gene09451.t1 | MTR_3g098310 | 40.870 | 230 | 121 | 6 | 37 | 259 | 298 | 519 | 5.17e-43 | 155 |
| MS.gene09451.t1 | MTR_3g098310 | 40.870 | 230 | 121 | 6 | 37 | 259 | 298 | 519 | 7.44e-43 | 155 |
| MS.gene09451.t1 | MTR_3g461000 | 39.823 | 226 | 121 | 6 | 37 | 258 | 172 | 386 | 7.95e-41 | 149 |
| MS.gene09451.t1 | MTR_6g048860 | 40.260 | 154 | 79 | 5 | 22 | 171 | 121 | 265 | 6.21e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09451.t1 | AT1G74310 | 70.472 | 254 | 64 | 4 | 8 | 259 | 135 | 379 | 4.19e-110 | 336 |
| MS.gene09451.t1 | AT1G74310 | 70.472 | 254 | 64 | 4 | 8 | 259 | 135 | 379 | 1.96e-109 | 336 |
| MS.gene09451.t1 | AT4G14670 | 57.937 | 252 | 84 | 4 | 9 | 258 | 112 | 343 | 6.35e-85 | 265 |
| MS.gene09451.t1 | AT2G25140 | 55.603 | 232 | 90 | 5 | 31 | 258 | 236 | 458 | 3.34e-75 | 246 |
| MS.gene09451.t1 | AT5G15450 | 56.332 | 229 | 87 | 5 | 34 | 258 | 234 | 453 | 6.93e-74 | 243 |
| MS.gene09451.t1 | AT3G48870 | 50.216 | 231 | 99 | 6 | 33 | 258 | 243 | 462 | 3.84e-62 | 210 |
| MS.gene09451.t1 | AT3G48870 | 50.216 | 231 | 99 | 6 | 33 | 258 | 274 | 493 | 5.14e-62 | 210 |
| MS.gene09451.t1 | AT3G48870 | 50.216 | 231 | 99 | 6 | 33 | 258 | 274 | 493 | 5.14e-62 | 210 |
| MS.gene09451.t1 | AT3G48870 | 50.216 | 231 | 99 | 6 | 33 | 258 | 274 | 493 | 5.14e-62 | 210 |
| MS.gene09451.t1 | AT5G50920 | 49.782 | 229 | 101 | 6 | 34 | 258 | 254 | 472 | 1.10e-61 | 209 |
| MS.gene09451.t1 | AT5G51070 | 43.723 | 231 | 115 | 6 | 36 | 259 | 270 | 492 | 4.94e-46 | 164 |
| MS.gene09451.t1 | AT3G45450 | 38.298 | 188 | 85 | 5 | 47 | 230 | 149 | 309 | 1.84e-28 | 111 |
Find 48 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGAAATCCATCTTGTTCT+TGG | 0.215023 | 6.4:-23617980 | MS.gene09451:CDS |
| ATCCATCTTGTTCTTGGTTC+TGG | 0.248495 | 6.4:-23617974 | MS.gene09451:CDS |
| TGAAGAATACTAATGGTATT+TGG | 0.251089 | 6.4:+23617779 | None:intergenic |
| GGGAAGCTTGACCCTGTTAT+TGG | 0.256411 | 6.4:-23618307 | MS.gene09451:CDS |
| TGTCGTTGTTTCTACTTTCT+AGG | 0.256682 | 6.4:-23618447 | MS.gene09451:CDS |
| AAGAAAGTTGAAAGTGCTTT+TGG | 0.322493 | 6.4:-23618385 | MS.gene09451:CDS |
| TCATGATCACGTCCAATAAC+AGG | 0.327578 | 6.4:+23618295 | None:intergenic |
| AATACCATTAGTATTCTTCA+TGG | 0.331483 | 6.4:-23617776 | MS.gene09451:CDS |
| AGGATATTTGCTTTAGATAT+CGG | 0.333330 | 6.4:-23618106 | MS.gene09451:CDS |
| CTTGGTTCTGGTAGAATTGA+AGG | 0.374889 | 6.4:-23617962 | MS.gene09451:CDS |
| TATTCCATGAAGAATACTAA+TGG | 0.387671 | 6.4:+23617772 | None:intergenic |
| GAAAATGGTAAAACAGATCT+TGG | 0.397235 | 6.4:-23618145 | MS.gene09451:CDS |
| CAACAACAATTGAAGATAAC+AGG | 0.399167 | 6.4:-23617871 | MS.gene09451:CDS |
| AGAAATTTGGTTGAACGAGC+AGG | 0.401042 | 6.4:-23618328 | MS.gene09451:CDS |
| GAAAAGCTTTATCGTAAAGA+AGG | 0.435175 | 6.4:-23618409 | MS.gene09451:CDS |
| GTCGTTGTTTCTACTTTCTA+GGG | 0.438958 | 6.4:-23618446 | MS.gene09451:CDS |
| CTTATTCTCGTAGGAGAGCC+AGG | 0.459956 | 6.4:-23618226 | MS.gene09451:CDS |
| AAGATGAAGCATTTGAGAGG+AGG | 0.491991 | 6.4:-23617835 | MS.gene09451:CDS |
| ATTTGAGAGGAGGTTTGTAC+AGG | 0.503602 | 6.4:-23617825 | MS.gene09451:CDS |
| GAGATATACGAGGATCACTA+TGG | 0.507055 | 6.4:-23617746 | MS.gene09451:CDS |
| CTCGTAGGAGAGCCAGGTGT+TGG | 0.518280 | 6.4:-23618220 | MS.gene09451:CDS |
| TATTTGCTAGTGCTAAGAGC+GGG | 0.518643 | 6.4:-23618078 | MS.gene09451:CDS |
| CTAATGGTATTTGGAACACT+AGG | 0.519006 | 6.4:+23617788 | None:intergenic |
| GGTGGATATCTTAGATGCAT+TGG | 0.521176 | 6.4:-23617896 | MS.gene09451:CDS |
| GTTGTTGAGGCTCAAAGGAT+AGG | 0.524091 | 6.4:-23618187 | MS.gene09451:CDS |
| CTGTTGTTGTTGAGGCTCAA+AGG | 0.524599 | 6.4:-23618192 | MS.gene09451:CDS |
| GACGTGATCATGAGATAGAA+AGG | 0.534616 | 6.4:-23618285 | MS.gene09451:CDS |
| AAAAGCTTTATCGTAAAGAA+GGG | 0.549228 | 6.4:-23618408 | MS.gene09451:CDS |
| AAGTGGAAGAAGCTCAAGGA+AGG | 0.566087 | 6.4:-23618018 | MS.gene09451:CDS |
| GAAATTTGGTTGAACGAGCA+GGG | 0.567062 | 6.4:-23618327 | MS.gene09451:CDS |
| AAGAACAATCTTATTCTCGT+AGG | 0.582720 | 6.4:-23618235 | MS.gene09451:CDS |
| TTGGAAATATTGCTAATGCA+AGG | 0.586556 | 6.4:-23618126 | MS.gene09451:CDS |
| CTCAAACCGATGCTTGATCG+TGG | 0.593441 | 6.4:-23617917 | MS.gene09451:CDS |
| TACCAGAACCAAGAACAAGA+TGG | 0.602841 | 6.4:+23617972 | None:intergenic |
| AAAGAAGTGGAAGAAGCTCA+AGG | 0.604695 | 6.4:-23618022 | MS.gene09451:CDS |
| GAGGCTCAAAGGATAGGTAG+AGG | 0.609520 | 6.4:-23618181 | MS.gene09451:CDS |
| TGGTAGAATTGAAGGATCAA+CGG | 0.613812 | 6.4:-23617954 | MS.gene09451:CDS |
| ATTTGCTAGTGCTAAGAGCG+GGG | 0.616633 | 6.4:-23618077 | MS.gene09451:CDS |
| TTGCTAGTGCTAAGAGCGGG+GGG | 0.617655 | 6.4:-23618075 | MS.gene09451:CDS |
| TTTGCTAGTGCTAAGAGCGG+GGG | 0.619297 | 6.4:-23618076 | MS.gene09451:CDS |
| GAGGTTTGTACAGGTTGATG+TGG | 0.620416 | 6.4:-23617816 | MS.gene09451:CDS |
| CATGATCACGTCCAATAACA+GGG | 0.621278 | 6.4:+23618296 | None:intergenic |
| AAACCGATGCTTGATCGTGG+TGG | 0.626613 | 6.4:-23617914 | MS.gene09451:CDS |
| TATCCACCACGATCAAGCAT+CGG | 0.653402 | 6.4:+23617911 | None:intergenic |
| AGTGGAAGAAGCTCAAGGAA+GGG | 0.658878 | 6.4:-23618017 | MS.gene09451:CDS |
| TGCTAGTGCTAAGAGCGGGG+GGG | 0.660044 | 6.4:-23618074 | MS.gene09451:CDS |
| TTATTTGCTAGTGCTAAGAG+CGG | 0.675331 | 6.4:-23618079 | MS.gene09451:CDS |
| TGGAATAAAAGAGATATACG+AGG | 0.757207 | 6.4:-23617756 | MS.gene09451:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTCAAGTTTTGAAAAAATA+TGG | - | chr6.4:23617793-23617812 | MS.gene09451:CDS | 15.0% |
| !! | GAAAAAATATGGTAGAAATT+TGG | - | chr6.4:23617804-23617823 | MS.gene09451:CDS | 20.0% |
| ! | AATACCATTAGTATTCTTCA+TGG | - | chr6.4:23618369-23618388 | MS.gene09451:CDS | 25.0% |
| ! | GGTAAAAAAGATCTTGAAAA+TGG | - | chr6.4:23617985-23618004 | MS.gene09451:CDS | 25.0% |
| ! | TATTCCATGAAGAATACTAA+TGG | + | chr6.4:23618376-23618395 | None:intergenic | 25.0% |
| ! | TGAAGAATACTAATGGTATT+TGG | + | chr6.4:23618369-23618388 | None:intergenic | 25.0% |
| !! | AAAAGCTTTATCGTAAAGAA+GGG | - | chr6.4:23617737-23617756 | MS.gene09451:CDS | 25.0% |
| !! | AGGATATTTGCTTTAGATAT+CGG | - | chr6.4:23618039-23618058 | MS.gene09451:CDS | 25.0% |
| !! | TTGTCGTGATTTTATCAAAA+AGG | - | chr6.4:23617884-23617903 | MS.gene09451:CDS | 25.0% |
| AAGAACAATCTTATTCTCGT+AGG | - | chr6.4:23617910-23617929 | MS.gene09451:CDS | 30.0% | |
| AATGAAATCCATCTTGTTCT+TGG | - | chr6.4:23618165-23618184 | MS.gene09451:CDS | 30.0% | |
| CAACAACAATTGAAGATAAC+AGG | - | chr6.4:23618274-23618293 | MS.gene09451:CDS | 30.0% | |
| GAAAATGGTAAAACAGATCT+TGG | - | chr6.4:23618000-23618019 | MS.gene09451:CDS | 30.0% | |
| TGGAATAAAAGAGATATACG+AGG | - | chr6.4:23618389-23618408 | MS.gene09451:CDS | 30.0% | |
| TTGGAAATATTGCTAATGCA+AGG | - | chr6.4:23618019-23618038 | MS.gene09451:CDS | 30.0% | |
| ! | AAAAAGATGAAGCATTTGAG+AGG | - | chr6.4:23618307-23618326 | MS.gene09451:CDS | 30.0% |
| ! | GAAAAGCTTTATCGTAAAGA+AGG | - | chr6.4:23617736-23617755 | MS.gene09451:CDS | 30.0% |
| !!! | AAGAAAGTTGAAAGTGCTTT+TGG | - | chr6.4:23617760-23617779 | MS.gene09451:CDS | 30.0% |
| TGGTAGAATTGAAGGATCAA+CGG | - | chr6.4:23618191-23618210 | MS.gene09451:CDS | 35.0% | |
| ! | CTAATGGTATTTGGAACACT+AGG | + | chr6.4:23618360-23618379 | None:intergenic | 35.0% |
| ! | TGGAAAAACTGTTGTTGTTG+AGG | - | chr6.4:23617945-23617964 | MS.gene09451:CDS | 35.0% |
| ! | TTATTTGCTAGTGCTAAGAG+CGG | - | chr6.4:23618066-23618085 | MS.gene09451:CDS | 35.0% |
| !! | GTCGTTGTTTCTACTTTCTA+GGG | - | chr6.4:23617699-23617718 | MS.gene09451:CDS | 35.0% |
| !! | TGTCGTTGTTTCTACTTTCT+AGG | - | chr6.4:23617698-23617717 | MS.gene09451:CDS | 35.0% |
| !!! | GAAGGAAGTTTTGAAAGAAG+TGG | - | chr6.4:23618110-23618129 | MS.gene09451:CDS | 35.0% |
| AAAGAAGTGGAAGAAGCTCA+AGG | - | chr6.4:23618123-23618142 | MS.gene09451:CDS | 40.0% | |
| AGAAATTTGGTTGAACGAGC+AGG | - | chr6.4:23617817-23617836 | MS.gene09451:CDS | 40.0% | |
| ATCCATCTTGTTCTTGGTTC+TGG | - | chr6.4:23618171-23618190 | MS.gene09451:CDS | 40.0% | |
| ATTTGAGAGGAGGTTTGTAC+AGG | - | chr6.4:23618320-23618339 | MS.gene09451:CDS | 40.0% | |
| CATGATCACGTCCAATAACA+GGG | + | chr6.4:23617852-23617871 | None:intergenic | 40.0% | |
| GAAATTTGGTTGAACGAGCA+GGG | - | chr6.4:23617818-23617837 | MS.gene09451:CDS | 40.0% | |
| GACGTGATCATGAGATAGAA+AGG | - | chr6.4:23617860-23617879 | MS.gene09451:CDS | 40.0% | |
| GAGATATACGAGGATCACTA+TGG | - | chr6.4:23618399-23618418 | MS.gene09451:CDS | 40.0% | |
| GGTGGATATCTTAGATGCAT+TGG | - | chr6.4:23618249-23618268 | MS.gene09451:CDS | 40.0% | |
| TACCAGAACCAAGAACAAGA+TGG | + | chr6.4:23618176-23618195 | None:intergenic | 40.0% | |
| TCATGATCACGTCCAATAAC+AGG | + | chr6.4:23617853-23617872 | None:intergenic | 40.0% | |
| ! | AAGATGAAGCATTTGAGAGG+AGG | - | chr6.4:23618310-23618329 | MS.gene09451:CDS | 40.0% |
| ! | ACAACAGTTTTTCCAACACC+TGG | + | chr6.4:23617940-23617959 | None:intergenic | 40.0% |
| ! | TATTTGCTAGTGCTAAGAGC+GGG | - | chr6.4:23618067-23618086 | MS.gene09451:CDS | 40.0% |
| !! | CTTGGTTCTGGTAGAATTGA+AGG | - | chr6.4:23618183-23618202 | MS.gene09451:CDS | 40.0% |
| !! | GGATTTTGAAGAGAGGTTGA+AGG | - | chr6.4:23618092-23618111 | MS.gene09451:CDS | 40.0% |
| AAGTGGAAGAAGCTCAAGGA+AGG | - | chr6.4:23618127-23618146 | MS.gene09451:CDS | 45.0% | |
| AGTGGAAGAAGCTCAAGGAA+GGG | - | chr6.4:23618128-23618147 | MS.gene09451:CDS | 45.0% | |
| GTTGTTGAGGCTCAAAGGAT+AGG | - | chr6.4:23617958-23617977 | MS.gene09451:CDS | 45.0% | |
| TATCCACCACGATCAAGCAT+CGG | + | chr6.4:23618237-23618256 | None:intergenic | 45.0% | |
| ! | ATTTGCTAGTGCTAAGAGCG+GGG | - | chr6.4:23618068-23618087 | MS.gene09451:CDS | 45.0% |
| ! | CTGTTGTTGTTGAGGCTCAA+AGG | - | chr6.4:23617953-23617972 | MS.gene09451:CDS | 45.0% |
| !! | GAGGTTTGTACAGGTTGATG+TGG | - | chr6.4:23618329-23618348 | MS.gene09451:CDS | 45.0% |
| AAACCGATGCTTGATCGTGG+TGG | - | chr6.4:23618231-23618250 | MS.gene09451:CDS | 50.0% | |
| CTCAAACCGATGCTTGATCG+TGG | - | chr6.4:23618228-23618247 | MS.gene09451:CDS | 50.0% | |
| CTTATTCTCGTAGGAGAGCC+AGG | - | chr6.4:23617919-23617938 | MS.gene09451:CDS | 50.0% | |
| GAGGCTCAAAGGATAGGTAG+AGG | - | chr6.4:23617964-23617983 | MS.gene09451:CDS | 50.0% | |
| GGGAAGCTTGACCCTGTTAT+TGG | - | chr6.4:23617838-23617857 | MS.gene09451:CDS | 50.0% | |
| TTTGCTAGTGCTAAGAGCGG+GGG | - | chr6.4:23618069-23618088 | MS.gene09451:CDS | 50.0% | |
| TTGCTAGTGCTAAGAGCGGG+GGG | - | chr6.4:23618070-23618089 | MS.gene09451:CDS | 55.0% | |
| CTCGTAGGAGAGCCAGGTGT+TGG | - | chr6.4:23617925-23617944 | MS.gene09451:CDS | 60.0% | |
| TGCTAGTGCTAAGAGCGGGG+GGG | - | chr6.4:23618071-23618090 | MS.gene09451:CDS | 60.0% | |
| !! | GCGGGGGGGATTTTGAAGAG+AGG | - | chr6.4:23618085-23618104 | MS.gene09451:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 23617694 | 23618473 | 23617694 | ID=MS.gene09451 |
| chr6.4 | mRNA | 23617694 | 23618473 | 23617694 | ID=MS.gene09451.t1;Parent=MS.gene09451 |
| chr6.4 | exon | 23617694 | 23618473 | 23617694 | ID=MS.gene09451.t1.exon1;Parent=MS.gene09451.t1 |
| chr6.4 | CDS | 23617694 | 23618473 | 23617694 | ID=cds.MS.gene09451.t1;Parent=MS.gene09451.t1 |
| Gene Sequence |
| Protein sequence |
