AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20921

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20921.t1 MTR_3g008640 96.525 518 18 0 1 518 10 527 0.0 1061
MS.gene20921.t1 MTR_3g008720 96.139 518 20 0 1 518 10 527 0.0 1056
MS.gene20921.t1 MTR_3g008700 96.139 518 20 0 1 518 10 527 0.0 1033
MS.gene20921.t1 MTR_7g053360 95.946 518 21 0 1 518 10 527 0.0 1033
MS.gene20921.t1 MTR_3g033690 94.208 518 29 1 1 518 10 526 0.0 1033
MS.gene20921.t1 MTR_4g035980 66.008 506 160 5 23 518 36 539 0.0 701
MS.gene20921.t1 MTR_4g025690 52.816 515 223 11 9 518 14 513 4.97e-179 513
MS.gene20921.t1 MTR_4g025730 49.312 509 229 13 17 518 23 509 7.53e-166 479
MS.gene20921.t1 MTR_8g104655 45.720 514 249 8 27 518 36 541 1.26e-155 454
MS.gene20921.t1 MTR_7g108905 48.636 440 205 7 93 514 116 552 3.27e-143 423
MS.gene20921.t1 MTR_8g104645 56.932 339 141 2 184 518 35 372 4.30e-139 406
MS.gene20921.t1 MTR_7g050980 47.379 477 226 11 58 518 88 555 1.03e-138 412
MS.gene20921.t1 MTR_7g050870 48.238 454 212 11 79 518 110 554 5.42e-136 405
MS.gene20921.t1 MTR_7g050950 48.970 437 203 11 94 518 125 553 6.40e-135 402
MS.gene20921.t1 MTR_4g087980 48.848 434 208 6 93 518 89 516 8.97e-134 398
MS.gene20921.t1 MTR_2g044880 45.747 435 223 7 93 518 95 525 8.95e-131 390
MS.gene20921.t1 MTR_3g091380 45.870 460 216 10 88 518 142 597 7.58e-130 390
MS.gene20921.t1 MTR_8g104580 41.634 514 237 10 27 518 28 500 1.49e-129 386
MS.gene20921.t1 MTR_8g023310 42.614 528 257 12 27 518 72 589 1.04e-128 387
MS.gene20921.t1 MTR_4g087970 45.475 442 227 6 87 518 80 517 1.23e-128 385
MS.gene20921.t1 MTR_4g025670 45.370 432 220 6 93 518 68 489 4.96e-127 380
MS.gene20921.t1 MTR_1g008140 43.871 465 225 10 88 518 145 607 1.03e-122 372
MS.gene20921.t1 MTR_7g112580 44.270 445 218 8 93 518 109 542 6.13e-122 369
MS.gene20921.t1 MTR_1g083520 41.199 534 280 13 1 518 13 528 6.68e-122 368
MS.gene20921.t1 MTR_2g044810 38.462 533 296 11 1 518 19 534 1.60e-121 367
MS.gene20921.t1 MTR_7g108900 42.827 467 222 9 93 518 98 560 3.77e-121 367
MS.gene20921.t1 MTR_8g042900 41.313 518 276 10 20 518 73 581 1.24e-120 366
MS.gene20921.t1 MTR_4g124520 38.264 507 281 9 21 518 38 521 9.41e-120 362
MS.gene20921.t1 MTR_1g088020 40.075 534 268 12 27 518 32 555 4.93e-118 359
MS.gene20921.t1 MTR_3g010780 42.650 483 247 11 57 518 101 574 1.40e-117 358
MS.gene20921.t1 MTR_1g054880 39.630 540 260 13 27 518 37 558 2.80e-117 357
MS.gene20921.t1 MTR_8g042910 51.652 333 154 4 191 518 282 612 1.07e-116 357
MS.gene20921.t1 MTR_7g108875 39.623 530 275 11 27 518 42 564 2.01e-116 355
MS.gene20921.t1 MTR_7g108890 39.245 530 277 9 27 518 40 562 7.49e-113 346
MS.gene20921.t1 MTR_1g088225 39.513 534 269 13 27 518 32 553 4.67e-112 343
MS.gene20921.t1 MTR_3g010770 51.506 332 154 5 192 518 246 575 6.13e-112 344
MS.gene20921.t1 MTR_0458s0030 56.475 278 115 4 244 518 40 314 1.15e-107 324
MS.gene20921.t1 MTR_1g088040 56.475 278 115 4 244 518 40 314 1.15e-107 324
MS.gene20921.t1 MTR_8g023150 39.423 520 269 12 27 518 81 582 8.02e-107 331
MS.gene20921.t1 MTR_4g124550 39.741 463 244 8 83 518 139 593 2.20e-104 325
MS.gene20921.t1 MTR_0618s0020 61.842 228 86 1 291 518 2 228 8.61e-103 308
MS.gene20921.t1 MTR_1g083470 34.578 509 308 8 27 514 62 566 1.79e-100 314
MS.gene20921.t1 MTR_1g083500 34.578 509 308 8 27 514 62 566 1.79e-100 314
MS.gene20921.t1 MTR_4g005170 38.375 443 247 8 94 514 133 571 2.89e-98 310
MS.gene20921.t1 MTR_1g083510 38.147 464 242 12 94 517 124 582 9.19e-98 307
MS.gene20921.t1 MTR_1g083490 34.706 510 306 9 27 514 67 571 9.70e-98 307
MS.gene20921.t1 MTR_4g005180 34.706 510 306 9 27 514 49 553 6.16e-97 305
MS.gene20921.t1 MTR_1g086210 37.238 478 279 12 49 518 105 569 2.45e-95 301
MS.gene20921.t1 MTR_4g120290 33.959 533 302 11 19 517 55 571 2.89e-95 301
MS.gene20921.t1 MTR_1g086190 36.561 506 282 11 40 518 70 563 1.44e-93 296
MS.gene20921.t1 MTR_4g120280 34.908 487 269 7 55 517 109 571 7.55e-93 295
MS.gene20921.t1 MTR_7g112590 35.283 530 294 15 27 517 63 582 7.57e-93 295
MS.gene20921.t1 MTR_2g044810 52.362 254 119 2 265 518 214 465 8.79e-93 291
MS.gene20921.t1 MTR_2g436040 32.839 539 318 13 2 515 24 543 6.13e-92 293
MS.gene20921.t1 MTR_3g095920 34.807 543 303 14 1 507 26 553 1.30e-91 291
MS.gene20921.t1 MTR_8g088610 42.690 342 191 3 175 515 36 373 1.52e-90 282
MS.gene20921.t1 MTR_2g044660 46.815 314 160 5 205 518 326 632 1.63e-88 285
MS.gene20921.t1 MTR_8g093460 35.824 522 288 15 19 510 39 543 1.93e-88 282
MS.gene20921.t1 MTR_7g110440 36.364 473 275 11 59 518 115 574 1.60e-86 278
MS.gene20921.t1 MTR_7g110490 36.147 462 251 11 93 513 111 569 4.86e-86 276
MS.gene20921.t1 MTR_7g110510 36.147 462 251 11 93 513 111 569 4.86e-86 276
MS.gene20921.t1 MTR_2g436050 33.643 538 292 14 19 517 52 563 6.34e-85 273
MS.gene20921.t1 MTR_5g083620 39.607 356 203 7 169 518 7 356 1.37e-81 258
MS.gene20921.t1 MTR_7g110560 33.680 481 282 8 45 518 99 549 5.38e-76 249
MS.gene20921.t1 MTR_2g105560 40.952 315 170 5 205 518 40 339 1.44e-75 242
MS.gene20921.t1 MTR_7g110420 33.333 477 266 10 93 518 111 586 1.54e-75 249
MS.gene20921.t1 MTR_7g107180 40.764 314 172 6 206 518 7 307 1.31e-72 233
MS.gene20921.t1 MTR_7g110430 32.051 468 238 13 93 518 111 540 1.54e-64 219
MS.gene20921.t1 MTR_7g111560 30.280 535 338 16 2 518 39 556 1.05e-63 217
MS.gene20921.t1 MTR_0458s0010 30.712 534 281 16 27 518 32 518 1.30e-57 200
MS.gene20921.t1 MTR_2g039530 39.482 309 170 7 179 485 26 319 2.19e-57 194
MS.gene20921.t1 MTR_6g012800 34.843 287 164 6 204 480 4 277 1.19e-54 186
MS.gene20921.t1 MTR_6g088810 35.890 326 180 10 194 507 69 377 8.61e-53 183
MS.gene20921.t1 MTR_6g024165 36.656 311 169 9 208 508 43 335 5.18e-52 180
MS.gene20921.t1 MTR_8g102380 35.417 336 181 12 188 509 66 379 9.87e-52 181
MS.gene20921.t1 MTR_1g086540 36.111 324 180 8 196 509 40 346 1.06e-51 179
MS.gene20921.t1 MTR_7g011530 34.035 285 161 6 208 480 8 277 5.95e-49 171
MS.gene20921.t1 MTR_7g102600 30.894 369 218 11 147 507 54 393 1.28e-46 167
MS.gene20921.t1 MTR_5g083590 32.063 315 161 5 205 518 6 268 1.34e-43 155
MS.gene20921.t1 MTR_8g102380 35.587 281 149 9 188 454 66 328 2.41e-43 157
MS.gene20921.t1 MTR_5g093970 33.224 304 188 5 206 509 43 331 6.05e-43 156
MS.gene20921.t1 MTR_6g042660 33.808 281 159 9 206 480 37 296 1.63e-42 154
MS.gene20921.t1 MTR_5g095490 34.091 308 174 9 212 507 67 357 2.17e-42 155
MS.gene20921.t1 MTR_7g092780 32.484 314 188 7 206 509 90 389 1.72e-41 153
MS.gene20921.t1 MTR_4g124740 31.402 328 190 10 189 507 38 339 4.26e-41 151
MS.gene20921.t1 MTR_5g012600 30.696 316 192 8 206 510 63 362 1.04e-39 148
MS.gene20921.t1 MTR_1g033050 33.091 275 160 7 214 480 26 284 1.51e-39 146
MS.gene20921.t1 MTR_5g088300 31.250 304 183 8 206 507 37 316 4.80e-37 139
MS.gene20921.t1 MTR_6g042690 33.099 284 162 8 206 480 39 303 4.93e-37 140
MS.gene20921.t1 MTR_2g033970 29.630 324 199 8 195 509 45 348 5.29e-36 137
MS.gene20921.t1 MTR_2g073390 30.000 340 199 10 187 507 42 361 1.66e-35 136
MS.gene20921.t1 MTR_5g088280 30.323 310 190 8 202 509 35 320 2.07e-35 135
MS.gene20921.t1 MTR_4g074290 29.155 343 212 9 180 507 35 361 2.49e-35 136
MS.gene20921.t1 MTR_5g088290 30.263 304 187 7 206 507 48 328 2.27e-33 130
MS.gene20921.t1 MTR_4g051810 31.636 275 169 5 206 480 37 292 1.14e-32 127
MS.gene20921.t1 MTR_0009s0350 28.994 338 207 9 186 507 41 361 2.57e-32 127
MS.gene20921.t1 MTR_0009s0340 28.994 338 207 9 186 507 41 361 2.57e-32 127
MS.gene20921.t1 MTR_2g436600 28.308 325 193 10 195 509 44 338 1.04e-29 119
MS.gene20921.t1 MTR_8g036225 29.577 284 173 8 206 480 33 298 1.98e-28 115
MS.gene20921.t1 MTR_6g025590 37.126 167 96 5 208 366 51 216 6.34e-25 103
MS.gene20921.t1 MTR_0408s0030 40.594 101 50 2 380 480 33 123 5.32e-18 81.6
MS.gene20921.t1 MTR_4g130790 32.143 196 117 6 204 388 20 210 1.30e-17 83.2
MS.gene20921.t1 MTR_2g436070 25.862 290 165 10 19 278 52 321 8.36e-16 79.7
MS.gene20921.t1 MTR_6g024200 37.600 125 66 3 383 507 164 276 8.36e-16 78.2
MS.gene20921.t1 MTR_7g102570 32.174 115 65 3 366 478 3 106 1.74e-11 61.6
MS.gene20921.t1 MTR_7g102580 40.698 86 41 3 281 361 73 153 2.04e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20921.t1 AT5G51490 55.556 504 209 7 26 518 37 536 0.0 579
MS.gene20921.t1 AT5G51500 55.490 510 212 10 20 518 35 540 0.0 577
MS.gene20921.t1 AT3G47400 55.019 518 216 8 17 518 78 594 0.0 572
MS.gene20921.t1 AT2G45220 50.098 509 238 10 11 518 18 511 5.48e-172 495
MS.gene20921.t1 AT4G00190 45.233 493 243 10 27 518 8 474 2.67e-140 413
MS.gene20921.t1 AT1G11580 46.957 460 229 7 64 514 100 553 1.90e-134 401
MS.gene20921.t1 AT1G23200 42.430 535 247 12 27 518 38 554 2.56e-134 400
MS.gene20921.t1 AT1G11580 46.957 460 229 7 64 514 121 574 4.60e-134 401
MS.gene20921.t1 AT5G53370 46.460 452 217 7 88 518 138 585 3.14e-131 394
MS.gene20921.t1 AT5G53370 46.460 452 217 7 88 518 174 621 1.44e-130 393
MS.gene20921.t1 AT3G60730 42.339 496 241 8 57 518 35 519 3.47e-129 386
MS.gene20921.t1 AT3G14310 45.781 474 211 8 87 518 123 592 4.96e-127 383
MS.gene20921.t1 AT4G02320 45.536 448 213 8 93 518 80 518 7.75e-127 380
MS.gene20921.t1 AT4G02300 44.671 441 225 8 93 518 96 532 2.95e-125 377
MS.gene20921.t1 AT3G59010 46.259 441 206 7 93 518 104 528 2.17e-124 374
MS.gene20921.t1 AT3G43270 44.619 446 224 7 93 518 83 525 2.27e-124 374
MS.gene20921.t1 AT4G02330 39.543 569 285 16 1 518 11 571 4.32e-124 375
MS.gene20921.t1 AT1G53830 45.652 460 212 8 93 518 132 587 6.61e-124 375
MS.gene20921.t1 AT1G02810 38.128 577 288 12 1 518 11 577 3.92e-123 373
MS.gene20921.t1 AT4G33220 43.107 457 225 7 93 518 71 523 1.35e-122 370
MS.gene20921.t1 AT3G49220 44.248 452 225 8 88 518 151 596 2.77e-122 371
MS.gene20921.t1 AT5G04960 41.199 517 269 12 18 516 63 562 4.14e-122 370
MS.gene20921.t1 AT3G49220 44.248 452 225 8 88 518 196 641 1.83e-121 370
MS.gene20921.t1 AT4G33220 51.156 346 166 3 173 518 60 402 2.40e-121 362
MS.gene20921.t1 AT5G20860 44.545 440 226 9 89 518 107 538 1.02e-120 365
MS.gene20921.t1 AT2G43050 44.931 434 214 7 93 518 100 516 2.38e-119 361
MS.gene20921.t1 AT2G26440 38.752 529 283 12 18 518 32 547 5.68e-116 353
MS.gene20921.t1 AT2G47550 52.836 335 151 4 189 518 226 558 1.35e-115 353
MS.gene20921.t1 AT3G05620 45.679 405 198 8 123 518 150 541 2.02e-115 352
MS.gene20921.t1 AT2G26450 39.457 479 271 8 49 518 143 611 2.05e-114 351
MS.gene20921.t1 AT3G10710 37.248 545 291 10 3 518 39 561 9.63e-114 348
MS.gene20921.t1 AT5G49180 41.277 470 249 9 69 514 100 566 1.09e-113 348
MS.gene20921.t1 AT3G05610 38.356 511 289 10 27 518 62 565 1.05e-111 346
MS.gene20921.t1 AT4G33230 42.271 414 218 7 123 518 196 606 2.48e-110 341
MS.gene20921.t1 AT5G27870 39.248 479 269 9 59 518 87 562 2.86e-110 344
MS.gene20921.t1 AT3G27980 45.226 398 208 7 125 518 106 497 2.43e-109 335
MS.gene20921.t1 AT3G10720 37.244 537 288 10 27 518 83 615 1.13e-107 334
MS.gene20921.t1 AT1G11590 40.685 467 238 11 62 518 87 524 1.78e-107 330
MS.gene20921.t1 AT4G03930 40.385 468 240 11 61 518 86 524 2.77e-107 330
MS.gene20921.t1 AT3G06830 38.037 489 269 11 59 516 81 566 1.31e-106 330
MS.gene20921.t1 AT5G04970 36.059 538 293 10 27 518 88 620 8.59e-105 327
MS.gene20921.t1 AT1G53840 35.955 534 279 13 20 512 70 581 1.76e-99 312
MS.gene20921.t1 AT3G10720 55.172 261 115 2 258 518 1 259 6.06e-99 300
MS.gene20921.t1 AT5G20860 40.573 419 203 8 89 497 107 489 4.30e-98 306
MS.gene20921.t1 AT3G14300 36.832 524 278 11 15 503 448 953 7.67e-98 317
MS.gene20921.t1 AT4G15980 42.700 363 200 6 159 516 341 700 1.02e-93 300
MS.gene20921.t1 AT4G15980 42.700 363 200 6 159 516 351 710 1.21e-93 300
MS.gene20921.t1 AT5G09760 48.495 299 140 5 200 485 235 532 3.46e-85 274
MS.gene20921.t1 AT5G09760 48.495 299 140 5 200 485 261 558 4.94e-85 274
MS.gene20921.t1 AT1G11370 49.393 247 124 1 203 449 43 288 2.27e-82 258
MS.gene20921.t1 AT5G64640 34.483 522 287 16 27 510 97 601 2.28e-81 265
MS.gene20921.t1 AT2G47030 39.045 356 199 6 177 518 237 588 4.53e-74 245
MS.gene20921.t1 AT2G47040 38.244 353 203 6 177 518 247 595 1.58e-72 241
MS.gene20921.t1 AT3G62170 31.537 501 288 12 50 518 111 588 4.46e-69 232
MS.gene20921.t1 AT2G36710 33.542 319 189 8 203 512 86 390 4.87e-50 177
MS.gene20921.t1 AT2G36700 34.202 307 182 7 206 507 42 333 9.44e-50 174
MS.gene20921.t1 AT2G21610 33.333 303 180 7 206 507 51 332 9.88e-50 174
MS.gene20921.t1 AT2G21610 33.333 303 180 7 206 507 56 337 1.75e-49 174
MS.gene20921.t1 AT5G19730 31.215 362 204 11 169 507 42 381 3.98e-49 174
MS.gene20921.t1 AT3G29090 33.333 285 163 6 208 480 9 278 3.66e-48 169
MS.gene20921.t1 AT1G05310 32.698 315 182 7 206 507 91 388 3.71e-44 160
MS.gene20921.t1 AT3G17060 31.269 323 191 8 195 507 35 336 3.76e-42 154
MS.gene20921.t1 AT5G07430 29.972 357 215 10 165 507 20 355 1.01e-40 150
MS.gene20921.t1 AT5G47500 29.936 314 193 7 206 508 61 358 4.00e-39 146
MS.gene20921.t1 AT1G44980 41.799 189 106 3 185 370 54 241 9.88e-39 142
MS.gene20921.t1 AT5G55590 33.333 318 183 10 202 507 78 378 1.51e-37 142
MS.gene20921.t1 AT5G07420 29.738 343 208 10 180 507 31 355 8.02e-37 140
MS.gene20921.t1 AT5G55590 32.653 294 165 8 177 450 45 325 1.47e-36 138
MS.gene20921.t1 AT3G24130 31.119 286 171 7 206 480 34 304 2.14e-36 138
MS.gene20921.t1 AT5G18990 29.568 301 175 9 195 480 21 299 8.74e-33 128
MS.gene20921.t1 AT5G61680 28.280 343 212 10 181 507 34 358 1.13e-32 128
MS.gene20921.t1 AT5G26810 29.252 294 170 8 206 480 34 308 3.18e-31 124
MS.gene20921.t1 AT1G69940 28.657 335 207 11 187 507 39 355 4.33e-31 124
MS.gene20921.t1 AT5G07410 28.657 335 207 11 187 507 39 355 4.46e-31 124
MS.gene20921.t1 AT2G19150 26.807 332 203 10 198 514 33 339 2.25e-29 119
MS.gene20921.t1 AT2G19150 28.195 266 159 7 198 450 33 279 3.58e-27 111
MS.gene20921.t1 AT2G47280 26.370 292 183 8 202 480 31 303 9.80e-26 108
MS.gene20921.t1 AT3G49220 29.730 222 130 7 88 288 196 412 7.02e-22 98.6

Find 100 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCACAATTGTCAAATT+AGG 0.096980 1.3:-60859555 MS.gene20921:CDS
TCATGCATTGAAAACCAATT+TGG 0.214818 1.3:+60860708 None:intergenic
CTTCTACCGGTGTGCTATAT+CGG 0.231966 1.3:-60859779 MS.gene20921:CDS
ACTTCTAGAACTTGTTATTA+TGG 0.232263 1.3:+60860461 None:intergenic
ACGGTTGCTGGACTCCTTGC+TGG 0.264051 1.3:-60859262 MS.gene20921:CDS
GCTCAAGACACATTGTATTA+TGG 0.265395 1.3:-60859382 MS.gene20921:CDS
TGTGAGGACCAGCAGTATTT+TGG 0.265892 1.3:+60859845 None:intergenic
ATTCCACAATTGTCAAATTA+GGG 0.271376 1.3:-60859554 MS.gene20921:CDS
AAGAAGGGTCATTTCCAAAT+TGG 0.272902 1.3:-60860722 MS.gene20921:CDS
TGATAAGTACAAAACCTTCT+TGG 0.297993 1.3:-60859506 MS.gene20921:CDS
GCATCAAAGTTTACGGTTGC+TGG 0.302438 1.3:-60859274 MS.gene20921:CDS
GGAATGAAATTCCAGTGTTT+TGG 0.313831 1.3:+60859572 None:intergenic
GTAAACTTTGATGCTTCATT+TGG 0.314281 1.3:+60859283 None:intergenic
TCAAACATAGAGTTGAATTC+AGG 0.324256 1.3:-60861514 MS.gene20921:CDS
TATGGAGAGTATGAGAATTA+TGG 0.325330 1.3:-60859364 MS.gene20921:CDS
GATCAAAGACCAGATGTAAA+TGG 0.333623 1.3:+60859211 None:intergenic
AAATGAAGCATCAAAGTTTA+CGG 0.334310 1.3:-60859281 MS.gene20921:CDS
TTTCAAGAAATGCATATTGA+TGG 0.359546 1.3:+60861160 None:intergenic
CAACAGCAAATAGAGTAAAA+TGG 0.362307 1.3:-60859330 MS.gene20921:CDS
GGCACCGTAGACTTCATCTT+TGG 0.365133 1.3:-60859694 MS.gene20921:CDS
CATCTCAACCGTACACTTGA+AGG 0.367169 1.3:-60861350 MS.gene20921:CDS
GATAAGTACAAAACCTTCTT+GGG 0.374659 1.3:-60859505 MS.gene20921:CDS
TACCGACAATGCTTCATCTA+TGG 0.380171 1.3:-60859715 MS.gene20921:CDS
TAAACTTTGATGCTTCATTT+GGG 0.423452 1.3:+60859284 None:intergenic
AGAAAATTGAAGATAAGAAA+AGG 0.431335 1.3:+60861654 None:intergenic
GAATGGGTCACCACGACCTT+GGG 0.437136 1.3:+60859594 None:intergenic
CCAATGTTATTGAGATGATA+AGG 0.438431 1.3:-60861193 MS.gene20921:CDS
TCCGTGCATAAAACGGTTCA+TGG 0.440471 1.3:-60861407 MS.gene20921:CDS
AGCCATGTTTGTACATCATT+TGG 0.450556 1.3:+60861299 None:intergenic
TTCTACCGGTGTGCTATATC+GGG 0.452629 1.3:-60859778 MS.gene20921:CDS
TGTGTTGTGTATTGTTTGCA+TGG 0.464751 1.3:+60861557 None:intergenic
AATCTTGTGGTGGCAAAAGA+TGG 0.472687 1.3:-60860648 MS.gene20921:CDS
GTGTATTGTTTGCATGGCTT+AGG 0.472890 1.3:+60861563 None:intergenic
CAGCCCTAATTTGACAATTG+TGG 0.474354 1.3:+60859551 None:intergenic
CTTCCTCAAGCAACATAGAT+TGG 0.484124 1.3:-60861607 MS.gene20921:CDS
GGAATGGGTCACCACGACCT+TGG 0.485955 1.3:+60859593 None:intergenic
GGGCAGTGATCATGTTAGCT+TGG 0.487370 1.3:+60859614 None:intergenic
ACAAGTTCTAGAAGTGTTCA+AGG 0.492055 1.3:-60860453 MS.gene20921:CDS
TGCCATAGATGAAGCATTGT+CGG 0.497633 1.3:+60859713 None:intergenic
CAGATCTCTCTGTCTTCTAC+CGG 0.498986 1.3:-60859792 MS.gene20921:CDS
TTTGTGATACATGTTAAGAA+AGG 0.500212 1.3:-60860558 MS.gene20921:CDS
AAACTCATCCGTGCATAAAA+CGG 0.500247 1.3:-60861414 MS.gene20921:CDS
ACAACTGTGCCATTTACATC+TGG 0.504212 1.3:-60859220 MS.gene20921:CDS
CAAACATAGAGTTGAATTCA+GGG 0.504754 1.3:-60861513 MS.gene20921:CDS
TCAATAGCCTCATCAAGAAA+AGG 0.505321 1.3:-60861632 MS.gene20921:CDS
CCTTATCATCTCAATAACAT+TGG 0.506543 1.3:+60861193 None:intergenic
CTATTTGCTGTTGATGAACC+AGG 0.508404 1.3:+60859340 None:intergenic
AATGACAACATTATGCTGAT+TGG 0.509190 1.3:-60860501 MS.gene20921:CDS
AATATCGAAACATGTAAAAG+TGG 0.509586 1.3:-60861260 MS.gene20921:CDS
TTCAGTAAAGTTCAATCTTG+TGG 0.510785 1.3:-60860661 MS.gene20921:CDS
TATTTAAGCCTTCAAGTGTA+CGG 0.516272 1.3:+60861342 None:intergenic
TGTTTGCATGGCTTAGGATG+TGG 0.520012 1.3:+60861569 None:intergenic
ACTCTTGAGTATTGTTGCCA+TGG 0.524355 1.3:+60859478 None:intergenic
TTGCCATGGCCTGCCCAAGA+AGG 0.533733 1.3:+60859492 None:intergenic
CAGAGAGATCTGAGGCTGAT+CGG 0.534495 1.3:+60859803 None:intergenic
ATGATCACTGCCCAAGGTCG+TGG 0.537091 1.3:-60859604 MS.gene20921:CDS
ACCCCATGGAGACCAACCTA+GGG 0.538985 1.3:+60859417 None:intergenic
TGCTGTTGCAGAGCTATAAG+TGG 0.543731 1.3:+60860422 None:intergenic
GCAAATAGAGTAAAATGGCC+TGG 0.544601 1.3:-60859325 MS.gene20921:CDS
GTGGTGGCAAAAGATGGTTC+TGG 0.550327 1.3:-60860642 MS.gene20921:CDS
TTTGCAAGAAAGCCTTTGGA+TGG 0.551261 1.3:-60859637 MS.gene20921:CDS
CAATCTATGTTGCTTGAGGA+AGG 0.551632 1.3:+60861608 None:intergenic
AAACCTTCTTGGGCAGGCCA+TGG 0.552962 1.3:-60859495 MS.gene20921:CDS
AGTACAAAACCTTCTTGGGC+AGG 0.555197 1.3:-60859501 MS.gene20921:CDS
ACTTATAGCTCTGCAACAGC+AGG 0.557059 1.3:-60860420 MS.gene20921:intron
TCACTTGCAGAAACAGAAGA+AGG 0.559810 1.3:-60860738 MS.gene20921:intron
ATAACCTTCCAAAATACTGC+TGG 0.563934 1.3:-60859853 MS.gene20921:CDS
CATCTTTGGCAACGCTGCAG+TGG 0.565515 1.3:-60859680 MS.gene20921:CDS
CCTCAAGCAACATAGATTGG+TGG 0.569379 1.3:-60861604 MS.gene20921:CDS
AACATTATGCTGATTGGTGA+CGG 0.581662 1.3:-60860495 MS.gene20921:CDS
ATGTTAGCTTGGCCATCCAA+AGG 0.583281 1.3:+60859625 None:intergenic
CCTTGCTGGACCAACATGGT+TGG 0.591137 1.3:-60859248 MS.gene20921:CDS
CACCAAATGATGTACAAACA+TGG 0.597110 1.3:-60861301 MS.gene20921:CDS
CACCCCATGGAGACCAACCT+AGG 0.600934 1.3:+60859416 None:intergenic
CCACCAATCTATGTTGCTTG+AGG 0.601277 1.3:+60861604 None:intergenic
CTAAGAGAAAATATAAGACA+AGG 0.604478 1.3:-60860581 MS.gene20921:CDS
TTTGTTATCACATGATAACC+AGG 0.611404 1.3:+60859307 None:intergenic
GGTGACCCATTCCAAAACAC+TGG 0.613436 1.3:-60859583 MS.gene20921:CDS
AGTAAAGTTCAATCTTGTGG+TGG 0.619446 1.3:-60860658 MS.gene20921:CDS
GCAAAATCAGTGTCACCCCA+TGG 0.619580 1.3:+60859403 None:intergenic
GTTGCCAAAGATGAAGTCTA+CGG 0.622853 1.3:+60859690 None:intergenic
CACTTGCAGAAACAGAAGAA+GGG 0.623150 1.3:-60860737 MS.gene20921:intron
GACTCCTTGCTGGACCAACA+TGG 0.624472 1.3:-60859252 MS.gene20921:CDS
GCCACAGCTTGACCTTTGTG+AGG 0.625981 1.3:+60859829 None:intergenic
CCAACCATGTTGGTCCAGCA+AGG 0.628774 1.3:+60859248 None:intergenic
GTTGTGCTAGCCAACCATGT+TGG 0.629798 1.3:+60859238 None:intergenic
GGGCTATCAAGACACTCTGA+TGG 0.632395 1.3:-60859758 MS.gene20921:CDS
TGTTTATAAAGAGAACATTG+AGG 0.633315 1.3:-60860535 MS.gene20921:CDS
GTAGAAGACAGAGAGATCTG+AGG 0.638257 1.3:+60859795 None:intergenic
AATACTGCTGGTCCTCACAA+AGG 0.646774 1.3:-60859841 MS.gene20921:CDS
GCAACAATACTCAAGAGTCA+TGG 0.646964 1.3:-60859473 MS.gene20921:CDS
CCCCTAGGTTGGTCTCCATG+GGG 0.648245 1.3:-60859418 MS.gene20921:CDS
TCCTCACAAAGGTCAAGCTG+TGG 0.651499 1.3:-60859830 MS.gene20921:CDS
GCTAACATGATCACTGCCCA+AGG 0.651878 1.3:-60859610 MS.gene20921:CDS
GAGTATGAGAATTATGGACC+TGG 0.657686 1.3:-60859358 MS.gene20921:CDS
CATTTCTTGAAACATAACAA+AGG 0.664591 1.3:-60861149 MS.gene20921:intron
CCCCATGGAGACCAACCTAG+GGG 0.685055 1.3:+60859418 None:intergenic
CCCATGGAGACCAACCTAGG+GGG 0.713906 1.3:+60859419 None:intergenic
AGTTCTAGAAGTGTTCAAGG+TGG 0.726357 1.3:-60860450 MS.gene20921:CDS
GATAGCCCGATATAGCACAC+CGG 0.756970 1.3:+60859773 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCATTAACTAATAATT+TGG + chr1.3:60860039-60860058 None:intergenic 10.0%
!! TATAACTATACATATTTATA+TGG + chr1.3:60860077-60860096 None:intergenic 10.0%
!!! AGTTATTCAATAATTTTTAA+CGG + chr1.3:60859798-60859817 None:intergenic 10.0%
!!! ATTGAATAACTTTATTTATA+AGG - chr1.3:60859807-60859826 MS.gene20921:CDS 10.0%
!! ATAAATTATCGAATGTTAAT+AGG - chr1.3:60859889-60859908 MS.gene20921:intron 15.0%
!! ATGTATAGTTATAACTTATA+TGG - chr1.3:60860084-60860103 MS.gene20921:intron 15.0%
!! TAAATTATCGAATGTTAATA+GGG - chr1.3:60859890-60859909 MS.gene20921:intron 15.0%
!! TAAATTATCGAATGTTTATT+TGG - chr1.3:60859852-60859871 MS.gene20921:CDS 15.0%
!!! GTATATATCTTCAATTTTTT+AGG - chr1.3:60859988-60860007 MS.gene20921:intron 15.0%
!!! TTTAAACACTGATTTAATTA+GGG + chr1.3:60860762-60860781 None:intergenic 15.0%
!!! TTTTAAACACTGATTTAATT+AGG + chr1.3:60860763-60860782 None:intergenic 15.0%
!! AGAAAATTGAAGATAAGAAA+AGG + chr1.3:60859221-60859240 None:intergenic 20.0%
!! AGGAAAAAAAGTTGTAAAAT+AGG - chr1.3:60859925-60859944 MS.gene20921:intron 20.0%
!! TATTTATAAGGAATGAAATG+CGG - chr1.3:60859819-60859838 MS.gene20921:CDS 20.0%
!! TTATAACAAGAACATAAAAC+AGG + chr1.3:60860495-60860514 None:intergenic 20.0%
!!! TTGCAAAAATGTTACAATTT+TGG + chr1.3:60861222-60861241 None:intergenic 20.0%
! AATATCGAAACATGTAAAAG+TGG - chr1.3:60859612-60859631 MS.gene20921:CDS 25.0%
! ATTCCACAATTGTCAAATTA+GGG - chr1.3:60861318-60861337 MS.gene20921:CDS 25.0%
! ATTTATAAGGAATGAAATGC+GGG - chr1.3:60859820-60859839 MS.gene20921:CDS 25.0%
! CATTTCTTGAAACATAACAA+AGG - chr1.3:60859723-60859742 MS.gene20921:CDS 25.0%
! CTAAATCAGTGAAGAAAATT+TGG + chr1.3:60860612-60860631 None:intergenic 25.0%
! CTAAGAGAAAATATAAGACA+AGG - chr1.3:60860291-60860310 MS.gene20921:intron 25.0%
! TAAACTTTGATGCTTCATTT+GGG + chr1.3:60861591-60861610 None:intergenic 25.0%
! TGTTTATAAAGAGAACATTG+AGG - chr1.3:60860337-60860356 MS.gene20921:intron 25.0%
! TTCTTGCAACTTATATAGTA+AGG - chr1.3:60860852-60860871 MS.gene20921:intron 25.0%
! TTTCAAGAAATGCATATTGA+TGG + chr1.3:60859715-60859734 None:intergenic 25.0%
! TTTGTGATACATGTTAAGAA+AGG - chr1.3:60860314-60860333 MS.gene20921:intron 25.0%
!! AAATGAAGCATCAAAGTTTA+CGG - chr1.3:60861591-60861610 MS.gene20921:CDS 25.0%
!! GTGTTTAAAATTATTTGCCA+CGG - chr1.3:60860773-60860792 MS.gene20921:intron 25.0%
!!! ACTTCTAGAACTTGTTATTA+TGG + chr1.3:60860414-60860433 None:intergenic 25.0%
!!! TCACTGATTTAGTTTTTTCT+AGG - chr1.3:60860620-60860639 MS.gene20921:CDS 25.0%
AAAATTATTTGCCACGGAAA+TGG - chr1.3:60860779-60860798 MS.gene20921:intron 30.0%
AATGACAACATTATGCTGAT+TGG - chr1.3:60860371-60860390 MS.gene20921:intron 30.0%
AGTTGTAAAATAGGAAGACA+CGG - chr1.3:60859934-60859953 MS.gene20921:intron 30.0%
CAAACATAGAGTTGAATTCA+GGG - chr1.3:60859359-60859378 MS.gene20921:CDS 30.0%
CAACAGCAAATAGAGTAAAA+TGG - chr1.3:60861542-60861561 MS.gene20921:CDS 30.0%
CATCTTTCATGTTCAATAGT+AGG + chr1.3:60860675-60860694 None:intergenic 30.0%
CATTCCACAATTGTCAAATT+AGG - chr1.3:60861317-60861336 MS.gene20921:CDS 30.0%
CCAATGTTATTGAGATGATA+AGG - chr1.3:60859679-60859698 MS.gene20921:CDS 30.0%
CCTTATCATCTCAATAACAT+TGG + chr1.3:60859682-60859701 None:intergenic 30.0%
CTATAAATCACAATCGTTGT+TGG + chr1.3:60860925-60860944 None:intergenic 30.0%
GATAAGTACAAAACCTTCTT+GGG - chr1.3:60861367-60861386 MS.gene20921:CDS 30.0%
GTAAACTTTGATGCTTCATT+TGG + chr1.3:60861592-60861611 None:intergenic 30.0%
GTTGAACTAATAACGAAAGT+TGG - chr1.3:60860533-60860552 MS.gene20921:CDS 30.0%
TATTTAAGCCTTCAAGTGTA+CGG + chr1.3:60859533-60859552 None:intergenic 30.0%
TCAAACATAGAGTTGAATTC+AGG - chr1.3:60859358-60859377 MS.gene20921:CDS 30.0%
TCATGCATTGAAAACCAATT+TGG + chr1.3:60860167-60860186 None:intergenic 30.0%
TGATAAGTACAAAACCTTCT+TGG - chr1.3:60861366-60861385 MS.gene20921:CDS 30.0%
TGATATTCATAGTCGTCTAA+TGG - chr1.3:60860827-60860846 MS.gene20921:intron 30.0%
TTCAGTAAAGTTCAATCTTG+TGG - chr1.3:60860211-60860230 MS.gene20921:intron 30.0%
! CTCTTGAATCTTTTAAATCC+AGG - chr1.3:60860965-60860984 MS.gene20921:intron 30.0%
! GTTTTGTACTTATCAACAAC+AGG + chr1.3:60861361-60861380 None:intergenic 30.0%
! TATGGAGAGTATGAGAATTA+TGG - chr1.3:60861508-60861527 MS.gene20921:CDS 30.0%
! TTTGTTATCACATGATAACC+AGG + chr1.3:60861568-60861587 None:intergenic 30.0%
!! CATGGTGATGAAAACTTTTA+TGG - chr1.3:60861417-60861436 MS.gene20921:CDS 30.0%
!!! AAATTCCAGTGTTTTGGAAT+GGG + chr1.3:60861297-60861316 None:intergenic 30.0%
!!! ATTGGTTTTCAATGCATGAA+AGG - chr1.3:60860168-60860187 MS.gene20921:intron 30.0%
AAACTCATCCGTGCATAAAA+CGG - chr1.3:60859458-60859477 MS.gene20921:CDS 35.0%
AAGAAGGGTCATTTCCAAAT+TGG - chr1.3:60860150-60860169 MS.gene20921:intron 35.0%
AGCCATGTTTGTACATCATT+TGG + chr1.3:60859576-60859595 None:intergenic 35.0%
AGTAAAGTTCAATCTTGTGG+TGG - chr1.3:60860214-60860233 MS.gene20921:intron 35.0%
ATAACCTTCCAAAATACTGC+TGG - chr1.3:60861019-60861038 MS.gene20921:intron 35.0%
CACCAAATGATGTACAAACA+TGG - chr1.3:60859571-60859590 MS.gene20921:CDS 35.0%
GCTCAAGACACATTGTATTA+TGG - chr1.3:60861490-60861509 MS.gene20921:CDS 35.0%
GTCATGCTAATATAAGCTTC+TGG - chr1.3:60860106-60860125 MS.gene20921:intron 35.0%
GTTGTAAAATAGGAAGACAC+GGG - chr1.3:60859935-60859954 MS.gene20921:intron 35.0%
TAATAGGGTTGAACATGAAG+AGG - chr1.3:60859905-60859924 MS.gene20921:intron 35.0%
TATTGAACATGAAAGATGCG+CGG - chr1.3:60860677-60860696 MS.gene20921:CDS 35.0%
TCAATAGCCTCATCAAGAAA+AGG - chr1.3:60859240-60859259 MS.gene20921:CDS 35.0%
TGTGTTGTGTATTGTTTGCA+TGG + chr1.3:60859318-60859337 None:intergenic 35.0%
! AACATTATGCTGATTGGTGA+CGG - chr1.3:60860377-60860396 MS.gene20921:intron 35.0%
! CATGGTAAGAGCTTCTTTTA+AGG + chr1.3:60859403-60859422 None:intergenic 35.0%
! CATTTTTGCAAGAAAGCCTT+TGG - chr1.3:60861231-60861250 MS.gene20921:CDS 35.0%
! GGAATGAAATTCCAGTGTTT+TGG + chr1.3:60861303-60861322 None:intergenic 35.0%
! TTCTTGTGCTTCTTTTTGCA+TGG + chr1.3:60859421-60859440 None:intergenic 35.0%
!! ACAAGTTCTAGAAGTGTTCA+AGG - chr1.3:60860419-60860438 MS.gene20921:intron 35.0%
!!! CTTTTAAATCCAGGCATTGA+TGG - chr1.3:60860974-60860993 MS.gene20921:intron 35.0%
!!! GAAATTCCAGTGTTTTGGAA+TGG + chr1.3:60861298-60861317 None:intergenic 35.0%
AAATGAAGTCCATCAATGCC+TGG + chr1.3:60860986-60861005 None:intergenic 40.0%
AATCTTGTGGTGGCAAAAGA+TGG - chr1.3:60860224-60860243 MS.gene20921:intron 40.0%
ATGGTGCTCTTACATATCAG+AGG - chr1.3:60860798-60860817 MS.gene20921:intron 40.0%
CACTTGCAGAAACAGAAGAA+GGG - chr1.3:60860135-60860154 MS.gene20921:intron 40.0%
CAGCCCTAATTTGACAATTG+TGG + chr1.3:60861324-60861343 None:intergenic 40.0%
CTTCCTCAAGCAACATAGAT+TGG - chr1.3:60859265-60859284 MS.gene20921:CDS 40.0%
GAGTATGAGAATTATGGACC+TGG - chr1.3:60861514-60861533 MS.gene20921:CDS 40.0%
GCAAATAGAGTAAAATGGCC+TGG - chr1.3:60861547-60861566 MS.gene20921:CDS 40.0%
GCAACAATACTCAAGAGTCA+TGG - chr1.3:60861399-60861418 MS.gene20921:CDS 40.0%
GTGTATTGTTTGCATGGCTT+AGG + chr1.3:60859312-60859331 None:intergenic 40.0%
GTTGCCAAAGATGAAGTCTA+CGG + chr1.3:60861185-60861204 None:intergenic 40.0%
TACCGACAATGCTTCATCTA+TGG - chr1.3:60861157-60861176 MS.gene20921:CDS 40.0%
TCACTTGCAGAAACAGAAGA+AGG - chr1.3:60860134-60860153 MS.gene20921:intron 40.0%
TGTTGGACAATGACACATGT+TGG + chr1.3:60860908-60860927 None:intergenic 40.0%
TTTGCAAGAAAGCCTTTGGA+TGG - chr1.3:60861235-60861254 MS.gene20921:CDS 40.0%
! ACCATGAACCGTTTTATGCA+CGG + chr1.3:60859469-60859488 None:intergenic 40.0%
! ACTCTTGAGTATTGTTGCCA+TGG + chr1.3:60861397-60861416 None:intergenic 40.0%
! CAATCTATGTTGCTTGAGGA+AGG + chr1.3:60859267-60859286 None:intergenic 40.0%
! GTAAGGACGCTTTAGATTGA+AGG - chr1.3:60860869-60860888 MS.gene20921:intron 40.0%
!! ACAACTGTGCCATTTACATC+TGG - chr1.3:60861652-60861671 MS.gene20921:CDS 40.0%
!! AGTTCTAGAAGTGTTCAAGG+TGG - chr1.3:60860422-60860441 MS.gene20921:CDS 40.0%
!! CTATTTGCTGTTGATGAACC+AGG + chr1.3:60861535-60861554 None:intergenic 40.0%
!! TGCCATAGATGAAGCATTGT+CGG + chr1.3:60861162-60861181 None:intergenic 40.0%
AATACTGCTGGTCCTCACAA+AGG - chr1.3:60861031-60861050 MS.gene20921:intron 45.0%
AGTACAAAACCTTCTTGGGC+AGG - chr1.3:60861371-60861390 MS.gene20921:CDS 45.0%
CAGATCTCTCTGTCTTCTAC+CGG - chr1.3:60861080-60861099 MS.gene20921:intron 45.0%
CATCTCAACCGTACACTTGA+AGG - chr1.3:60859522-60859541 MS.gene20921:CDS 45.0%
CCTCAAGCAACATAGATTGG+TGG - chr1.3:60859268-60859287 MS.gene20921:CDS 45.0%
CTTCTACCGGTGTGCTATAT+CGG - chr1.3:60861093-60861112 MS.gene20921:intron 45.0%
GCATCAAAGTTTACGGTTGC+TGG - chr1.3:60861598-60861617 MS.gene20921:CDS 45.0%
GTAGAAGACAGAGAGATCTG+AGG + chr1.3:60861080-60861099 None:intergenic 45.0%
TCCGTGCATAAAACGGTTCA+TGG - chr1.3:60859465-60859484 MS.gene20921:CDS 45.0%
TGCTGTTGCAGAGCTATAAG+TGG + chr1.3:60860453-60860472 None:intergenic 45.0%
TGTTTGCATGGCTTAGGATG+TGG + chr1.3:60859306-60859325 None:intergenic 45.0%
TTCTACCGGTGTGCTATATC+GGG - chr1.3:60861094-60861113 MS.gene20921:intron 45.0%
! ACTTATAGCTCTGCAACAGC+AGG - chr1.3:60860452-60860471 MS.gene20921:CDS 45.0%
! ATGTTAGCTTGGCCATCCAA+AGG + chr1.3:60861250-60861269 None:intergenic 45.0%
! CCACCAATCTATGTTGCTTG+AGG + chr1.3:60859271-60859290 None:intergenic 45.0%
! GGAAGGTCCTTTTCTTGATG+AGG + chr1.3:60859250-60859269 None:intergenic 45.0%
! TGTGAGGACCAGCAGTATTT+TGG + chr1.3:60861030-60861049 None:intergenic 45.0%
!! AGGACCAGCAGTATTTTGGA+AGG + chr1.3:60861026-60861045 None:intergenic 45.0%
!! ATGTAAGAGCACCATTTCCG+TGG + chr1.3:60860793-60860812 None:intergenic 45.0%
AAGTTGGCGACTCTAGTAGG+CGG - chr1.3:60860549-60860568 MS.gene20921:CDS 50.0%
CGAAAGTTGGCGACTCTAGT+AGG - chr1.3:60860546-60860565 MS.gene20921:CDS 50.0%
GATAGCCCGATATAGCACAC+CGG + chr1.3:60861102-60861121 None:intergenic 50.0%
GCAAAATCAGTGTCACCCCA+TGG + chr1.3:60861472-60861491 None:intergenic 50.0%
GCTAACATGATCACTGCCCA+AGG - chr1.3:60861262-60861281 MS.gene20921:CDS 50.0%
GGCACCGTAGACTTCATCTT+TGG - chr1.3:60861178-60861197 MS.gene20921:CDS 50.0%
GGGCAGTGATCATGTTAGCT+TGG + chr1.3:60861261-60861280 None:intergenic 50.0%
GGGCTATCAAGACACTCTGA+TGG - chr1.3:60861114-60861133 MS.gene20921:intron 50.0%
GGTGACCCATTCCAAAACAC+TGG - chr1.3:60861289-60861308 MS.gene20921:CDS 50.0%
GTGGTGGCAAAAGATGGTTC+TGG - chr1.3:60860230-60860249 MS.gene20921:intron 50.0%
GTTGTGCTAGCCAACCATGT+TGG + chr1.3:60861637-60861656 None:intergenic 50.0%
TCCTCACAAAGGTCAAGCTG+TGG - chr1.3:60861042-60861061 MS.gene20921:intron 50.0%
! CAGAGAGATCTGAGGCTGAT+CGG + chr1.3:60861072-60861091 None:intergenic 50.0%
AAACCTTCTTGGGCAGGCCA+TGG - chr1.3:60861377-60861396 MS.gene20921:CDS 55.0%
ACCCCATGGAGACCAACCTA+GGG + chr1.3:60861458-60861477 None:intergenic 55.0%
ATGATCACTGCCCAAGGTCG+TGG - chr1.3:60861268-60861287 MS.gene20921:CDS 55.0%
CATCTTTGGCAACGCTGCAG+TGG - chr1.3:60861192-60861211 MS.gene20921:CDS 55.0%
CCAACCATGTTGGTCCAGCA+AGG + chr1.3:60861627-60861646 None:intergenic 55.0%
CCTTGCTGGACCAACATGGT+TGG - chr1.3:60861624-60861643 MS.gene20921:CDS 55.0%
CTTTAGTGAGCCCCCTAGGT+TGG - chr1.3:60861443-60861462 MS.gene20921:CDS 55.0%
GAATGGGTCACCACGACCTT+GGG + chr1.3:60861281-60861300 None:intergenic 55.0%
GACACTTTAGTGAGCCCCCT+AGG - chr1.3:60861439-60861458 MS.gene20921:CDS 55.0%
GACTCCTTGCTGGACCAACA+TGG - chr1.3:60861620-60861639 MS.gene20921:CDS 55.0%
GCCACAGCTTGACCTTTGTG+AGG + chr1.3:60861046-60861065 None:intergenic 55.0%
ACGGTTGCTGGACTCCTTGC+TGG - chr1.3:60861610-60861629 MS.gene20921:CDS 60.0%
CACCCCATGGAGACCAACCT+AGG + chr1.3:60861459-60861478 None:intergenic 60.0%
CCCATGGAGACCAACCTAGG+GGG + chr1.3:60861456-60861475 None:intergenic 60.0%
CCCCATGGAGACCAACCTAG+GGG + chr1.3:60861457-60861476 None:intergenic 60.0%
GCGACTCTAGTAGGCGGAGA+TGG - chr1.3:60860555-60860574 MS.gene20921:CDS 60.0%
GGAATGGGTCACCACGACCT+TGG + chr1.3:60861282-60861301 None:intergenic 60.0%
TTGCCATGGCCTGCCCAAGA+AGG + chr1.3:60861383-60861402 None:intergenic 60.0%
!! CCCCCTAGGTTGGTCTCCAT+GGG - chr1.3:60861453-60861472 MS.gene20921:CDS 60.0%
!! CCCCTAGGTTGGTCTCCATG+GGG - chr1.3:60861454-60861473 MS.gene20921:CDS 60.0%
!! GCCCCCTAGGTTGGTCTCCA+TGG - chr1.3:60861452-60861471 MS.gene20921:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 60859213 60861681 60859213 ID=MS.gene20921
chr1.3 mRNA 60859213 60861681 60859213 ID=MS.gene20921.t1;Parent=MS.gene20921
chr1.3 exon 60861150 60861681 60861150 ID=MS.gene20921.t1.exon1;Parent=MS.gene20921.t1
chr1.3 CDS 60861150 60861681 60861150 ID=cds.MS.gene20921.t1;Parent=MS.gene20921.t1
chr1.3 exon 60860421 60860750 60860421 ID=MS.gene20921.t1.exon2;Parent=MS.gene20921.t1
chr1.3 CDS 60860421 60860750 60860421 ID=cds.MS.gene20921.t1;Parent=MS.gene20921.t1
chr1.3 exon 60859213 60859907 60859213 ID=MS.gene20921.t1.exon3;Parent=MS.gene20921.t1
chr1.3 CDS 60859213 60859907 60859213 ID=cds.MS.gene20921.t1;Parent=MS.gene20921.t1
Gene Sequence

>MS.gene20921

ATGCTCCTTTTCTTATCTTCAATTTTCTCAATAGCCTCATCAAGAAAAGGACCTTCCTCAAGCAACATAGATTGGTGGTGCAATTTAACACCACATCCTAAGCCATGCAAACAATACACAACACAAATGAACAATCATTTCAAAATCAAACATAGAGTTGAATTCAGGGAAATGCTTGTTCAATTAGCCTTAAAAGAAGCTCTTACCATGCAAAAAGAAGCACAAGAAAATTCTCAACAACAACAAAACTCATCCGTGCATAAAACGGTTCATGGTGATTGTTTGAAGCTATTTGAAATCACAATTTTTCATCTCAACCGTACACTTGAAGGCTTAAATAATGCAAGCAAAAATTGTTCACCAAATGATGTACAAACATGGCTCACAACTTCTCTCACAAATATCGAAACATGTAAAAGTGGAGCTTTAGAACTCAATGCTCAAGATTTCAATTTCATTATGCAAACCAATGTTATTGAGATGATAAGGAACATCTTAGCCATCAATATGCATTTCTTGAAACATAACAAAGGTAATAACAATTCTTTTATATTTCTCTCTTTAAAATATTAAGTATTGTCTCCGTTAAAAATTATTGAATAACTTTATTTATAAGGAATGAAATGCGGGTGTGAAAGATAAATTATCGAATGTTTATTTGGTCACATACATTAAAATAAATTATCGAATGTTAATAGGGTTGAACATGAAGAGGAAAAAAAGTTGTAAAATAGGAAGACACGGGAGCAATTATATATACTTTGTAGATGTTGAAGTATATATCTTCAATTTTTTAGGTATATAATGATAAATTTTGTATTCTCCAAATTATTAGTTAATGATAATATTGATGTCATCATACCATATAAATATGTATAGTTATAACTTATATGGTCATGCTAATATAAGCTTCTGGTTCTGTCACTTGCAGAAACAGAAGAAGGGTCATTTCCAAATTGGTTTTCAATGCATGAAAGGAAGCTTTTGCAATCTAAGTCTTCAGTAAAGTTCAATCTTGTGGTGGCAAAAGATGGTTCTGGACAGTACAAGACTGTGCAAGCTGCTTTAAACGCTGCTGCTAAGAGAAAATATAAGACAAGGTTTGTGATACATGTTAAGAAAGGTGTTTATAAAGAGAACATTGAGGTTGCTGTTCACAATGACAACATTATGCTGATTGGTGACGGAATGCAAAATACCATAATAACAAGTTCTAGAAGTGTTCAAGGTGGTTATACCACTTATAGCTCTGCAACAGCAGGTGAGTTATATTTCATTTCCTGTTTTATGTTCTTGTTATAAGAGTCACTACTAGAAAAAGTTGAACTAATAACGAAAGTTGGCGACTCTAGTAGGCGGAGATGGAATAGTGATGAATTTAATGTTTTTTCAATCTCCAAATTTTCTTCACTGATTTAGTTTTTTCTAGGTGTCTTTGTGTACAAGTAAATTTCATGCACCTACTATTGAACATGAAAGATGCGCGGAAAAAAATTGAGTGAAAATCTACAATCATTAAATGAGCTTTTGAAACTTAATTTTAAAGCCCTAATTAAATCAGTGTTTAAAATTATTTGCCACGGAAATGGTGCTCTTACATATCAGAGGTATATATGATATTCATAGTCGTCTAATGGCTTTCTTGCAACTTATATAGTAAGGACGCTTTAGATTGAAGGTGTATTTGTGTATCCAACATGTGTCATTGTCCAACAACGATTGTGATTTATAGTTTCTAATAGTTACATATTTCTCTTGAATCTTTTAAATCCAGGCATTGATGGACTTCATTTCATTGCACGTGACATAACCTTCCAAAATACTGCTGGTCCTCACAAAGGTCAAGCTGTGGCGCTCCGATCAGCCTCAGATCTCTCTGTCTTCTACCGGTGTGCTATATCGGGCTATCAAGACACTCTGATGGCTCATGCACAACGTCAATTCTACCGACAATGCTTCATCTATGGCACCGTAGACTTCATCTTTGGCAACGCTGCAGTGGTATTCCAAAATTGTAACATTTTTGCAAGAAAGCCTTTGGATGGCCAAGCTAACATGATCACTGCCCAAGGTCGTGGTGACCCATTCCAAAACACTGGAATTTCATTCCACAATTGTCAAATTAGGGCTGCATCAGATCTAAAGCCTGTTGTTGATAAGTACAAAACCTTCTTGGGCAGGCCATGGCAACAATACTCAAGAGTCATGGTGATGAAAACTTTTATGGACACTTTAGTGAGCCCCCTAGGTTGGTCTCCATGGGGTGACACTGATTTTGCTCAAGACACATTGTATTATGGAGAGTATGAGAATTATGGACCTGGTTCATCAACAGCAAATAGAGTAAAATGGCCTGGTTATCATGTGATAACAAACCCAAATGAAGCATCAAAGTTTACGGTTGCTGGACTCCTTGCTGGACCAACATGGTTGGCTAGCACAACTGTGCCATTTACATCTGGTCTTTGA
Protein sequence

>MS.gene20921.t1

MLLFLSSIFSIASSRKGPSSSNIDWWCNLTPHPKPCKQYTTQMNNHFKIKHRVEFREMLVQLALKEALTMQKEAQENSQQQQNSSVHKTVHGDCLKLFEITIFHLNRTLEGLNNASKNCSPNDVQTWLTTSLTNIETCKSGALELNAQDFNFIMQTNVIEMIRNILAINMHFLKHNKETEEGSFPNWFSMHERKLLQSKSSVKFNLVVAKDGSGQYKTVQAALNAAAKRKYKTRFVIHVKKGVYKENIEVAVHNDNIMLIGDGMQNTIITSSRSVQGGYTTYSSATAGIDGLHFIARDITFQNTAGPHKGQAVALRSASDLSVFYRCAISGYQDTLMAHAQRQFYRQCFIYGTVDFIFGNAAVVFQNCNIFARKPLDGQANMITAQGRGDPFQNTGISFHNCQIRAASDLKPVVDKYKTFLGRPWQQYSRVMVMKTFMDTLVSPLGWSPWGDTDFAQDTLYYGEYENYGPGSSTANRVKWPGYHVITNPNEASKFTVAGLLAGPTWLASTTVPFTSGL