Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20924.t1 | XP_013469134.1 | 82.7 | 388 | 9 | 2 | 1 | 330 | 1 | 388 | 6.70E-175 | 623.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20924.t1 | Q8VZQ2 | 28.8 | 416 | 155 | 12 | 1 | 328 | 1 | 363 | 6.6e-30 | 132.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20924.t1 | A0A072VMV6 | 82.7 | 388 | 9 | 2 | 1 | 330 | 1 | 388 | 4.8e-175 | 623.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene20924.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene20924 | 0.800398 | 1.47E-48 | -1.69E-46 |
| MS.gene050780 | MS.gene20924 | 0.808407 | 3.14E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene20924 | 0.846662 | 1.89E-59 | -1.69E-46 |
| MS.gene050808 | MS.gene20924 | 0.830403 | 3.00E-55 | -1.69E-46 |
| MS.gene051247 | MS.gene20924 | 0.817194 | 3.74E-52 | -1.69E-46 |
| MS.gene051265 | MS.gene20924 | 0.818495 | 1.90E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene20924 | 0.807642 | 4.57E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene20924 | 0.82453 | 7.68E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene20924 | 0.828997 | 6.58E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene20924 | 0.807774 | 4.29E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene20924 | 0.805177 | 1.51E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene20924 | 0.80749 | 4.93E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene20924 | 0.824509 | 7.77E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene20924 | 0.811014 | 8.65E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene20924 | 0.811506 | 6.77E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene20924 | 0.800637 | 1.31E-48 | -1.69E-46 |
| MS.gene056584 | MS.gene20924 | 0.807411 | 5.12E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene20924 | 0.821481 | 3.95E-53 | -1.69E-46 |
| MS.gene06001 | MS.gene20924 | 0.809872 | 1.53E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene20924 | 0.80011 | 1.68E-48 | -1.69E-46 |
| MS.gene060587 | MS.gene20924 | 0.800428 | 1.45E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20924.t1 | MTR_1g085640 | 80.670 | 388 | 17 | 3 | 1 | 330 | 1 | 388 | 0.0 | 618 |
| MS.gene20924.t1 | MTR_7g110830 | 46.939 | 392 | 93 | 18 | 1 | 311 | 1 | 358 | 3.20e-85 | 261 |
| MS.gene20924.t1 | MTR_1g085040 | 32.090 | 402 | 163 | 15 | 1 | 328 | 1 | 366 | 7.65e-31 | 120 |
| MS.gene20924.t1 | MTR_4g105130 | 42.748 | 131 | 18 | 3 | 1 | 74 | 1 | 131 | 1.73e-21 | 94.0 |
| MS.gene20924.t1 | MTR_3g077110 | 49.425 | 87 | 37 | 3 | 12 | 94 | 65 | 148 | 3.83e-17 | 81.6 |
| MS.gene20924.t1 | MTR_1g112760 | 55.385 | 65 | 25 | 1 | 12 | 72 | 66 | 130 | 5.29e-17 | 79.3 |
| MS.gene20924.t1 | MTR_7g076740 | 52.174 | 69 | 27 | 2 | 12 | 75 | 66 | 133 | 1.12e-15 | 76.6 |
| MS.gene20924.t1 | MTR_7g111290 | 42.446 | 139 | 23 | 3 | 1 | 82 | 1 | 139 | 1.65e-15 | 77.0 |
| MS.gene20924.t1 | MTR_4g073420 | 50.000 | 68 | 30 | 1 | 12 | 75 | 65 | 132 | 1.73e-15 | 75.9 |
| MS.gene20924.t1 | MTR_2g064160 | 34.615 | 130 | 27 | 3 | 1 | 73 | 1 | 129 | 2.66e-15 | 75.9 |
| MS.gene20924.t1 | MTR_2g089620 | 52.239 | 67 | 28 | 1 | 9 | 71 | 62 | 128 | 2.99e-15 | 75.9 |
| MS.gene20924.t1 | MTR_8g031360 | 55.224 | 67 | 24 | 2 | 12 | 73 | 67 | 132 | 3.77e-15 | 75.1 |
| MS.gene20924.t1 | MTR_6g009430 | 31.818 | 132 | 33 | 2 | 1 | 75 | 1 | 132 | 7.26e-15 | 74.7 |
| MS.gene20924.t1 | MTR_4g091490 | 53.125 | 64 | 26 | 1 | 12 | 71 | 66 | 129 | 7.40e-15 | 75.1 |
| MS.gene20924.t1 | MTR_2g011660 | 53.125 | 64 | 26 | 1 | 12 | 71 | 66 | 129 | 8.84e-15 | 74.7 |
| MS.gene20924.t1 | MTR_8g095390 | 52.941 | 68 | 28 | 2 | 12 | 75 | 65 | 132 | 9.82e-15 | 74.3 |
| MS.gene20924.t1 | MTR_3g045430 | 59.016 | 61 | 22 | 1 | 18 | 75 | 73 | 133 | 1.11e-14 | 73.9 |
| MS.gene20924.t1 | MTR_5g007370 | 50.746 | 67 | 29 | 1 | 12 | 74 | 65 | 131 | 2.13e-14 | 72.8 |
| MS.gene20924.t1 | MTR_4g478180 | 53.125 | 64 | 26 | 2 | 12 | 71 | 66 | 129 | 2.17e-14 | 73.6 |
| MS.gene20924.t1 | MTR_5g078140 | 53.731 | 67 | 25 | 2 | 12 | 73 | 65 | 130 | 3.11e-14 | 72.8 |
| MS.gene20924.t1 | MTR_3g074520 | 50.725 | 69 | 28 | 2 | 12 | 75 | 65 | 132 | 3.11e-14 | 72.8 |
| MS.gene20924.t1 | MTR_3g461490 | 51.471 | 68 | 29 | 1 | 12 | 75 | 65 | 132 | 3.69e-14 | 72.4 |
| MS.gene20924.t1 | MTR_7g111290 | 56.410 | 78 | 30 | 2 | 9 | 82 | 20 | 97 | 8.03e-14 | 71.6 |
| MS.gene20924.t1 | MTR_7g011170 | 52.239 | 67 | 28 | 2 | 12 | 74 | 65 | 131 | 9.79e-14 | 71.2 |
| MS.gene20924.t1 | MTR_3g065440 | 55.224 | 67 | 24 | 2 | 12 | 73 | 65 | 130 | 1.01e-13 | 71.2 |
| MS.gene20924.t1 | MTR_3g083540 | 50.000 | 68 | 30 | 2 | 9 | 72 | 91 | 158 | 1.08e-13 | 70.9 |
| MS.gene20924.t1 | MTR_4g102380 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 1.41e-13 | 70.1 |
| MS.gene20924.t1 | MTR_4g082290 | 54.688 | 64 | 25 | 2 | 12 | 71 | 65 | 128 | 1.67e-13 | 71.2 |
| MS.gene20924.t1 | MTR_4g082230 | 54.688 | 64 | 25 | 2 | 12 | 71 | 65 | 128 | 1.69e-13 | 71.2 |
| MS.gene20924.t1 | MTR_5g029840 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 3.61e-13 | 69.7 |
| MS.gene20924.t1 | MTR_5g042030 | 56.061 | 66 | 25 | 2 | 12 | 73 | 60 | 125 | 4.54e-13 | 68.9 |
| MS.gene20924.t1 | MTR_4g065017 | 54.386 | 57 | 20 | 2 | 9 | 60 | 67 | 122 | 1.12e-12 | 68.2 |
| MS.gene20924.t1 | MTR_3g097450 | 46.269 | 67 | 32 | 1 | 12 | 74 | 65 | 131 | 1.59e-12 | 67.4 |
| MS.gene20924.t1 | MTR_6g012690 | 51.562 | 64 | 27 | 2 | 12 | 71 | 65 | 128 | 1.85e-12 | 67.8 |
| MS.gene20924.t1 | MTR_3g028740 | 55.357 | 56 | 21 | 1 | 9 | 60 | 72 | 127 | 4.23e-12 | 66.6 |
| MS.gene20924.t1 | MTR_8g098860 | 32.203 | 118 | 23 | 2 | 1 | 61 | 1 | 118 | 1.40e-11 | 64.7 |
| MS.gene20924.t1 | MTR_2g097910 | 54.717 | 53 | 20 | 1 | 12 | 60 | 73 | 125 | 3.57e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20924.t1 | AT4G01680 | 48.026 | 152 | 21 | 3 | 1 | 95 | 1 | 151 | 4.15e-33 | 125 |
| MS.gene20924.t1 | AT1G09540 | 26.619 | 417 | 163 | 14 | 1 | 328 | 1 | 363 | 7.62e-27 | 108 |
| MS.gene20924.t1 | AT4G01680 | 42.105 | 152 | 30 | 4 | 1 | 95 | 1 | 151 | 5.62e-25 | 101 |
| MS.gene20924.t1 | AT1G57560 | 29.758 | 289 | 134 | 9 | 1 | 221 | 1 | 288 | 1.53e-24 | 101 |
| MS.gene20924.t1 | AT4G01680 | 56.818 | 88 | 33 | 3 | 12 | 95 | 77 | 163 | 4.59e-22 | 95.5 |
| MS.gene20924.t1 | AT4G01680 | 76.744 | 43 | 5 | 1 | 1 | 38 | 1 | 43 | 3.87e-14 | 72.8 |
| MS.gene20924.t1 | AT1G57560 | 42.105 | 133 | 20 | 3 | 1 | 76 | 1 | 133 | 5.70e-22 | 94.0 |
| MS.gene20924.t1 | AT5G26660 | 41.860 | 129 | 18 | 3 | 1 | 72 | 1 | 129 | 1.06e-21 | 94.7 |
| MS.gene20924.t1 | AT5G26660 | 61.538 | 65 | 21 | 2 | 12 | 72 | 34 | 98 | 2.89e-19 | 87.4 |
| MS.gene20924.t1 | AT5G60890 | 52.703 | 74 | 31 | 2 | 12 | 81 | 65 | 138 | 1.58e-17 | 82.0 |
| MS.gene20924.t1 | AT5G16600 | 52.564 | 78 | 33 | 1 | 12 | 85 | 65 | 142 | 2.50e-17 | 82.0 |
| MS.gene20924.t1 | AT1G66230 | 51.250 | 80 | 35 | 1 | 12 | 87 | 65 | 144 | 1.04e-16 | 79.3 |
| MS.gene20924.t1 | AT1G74430 | 51.562 | 64 | 27 | 1 | 12 | 71 | 65 | 128 | 1.61e-16 | 78.6 |
| MS.gene20924.t1 | AT5G10280 | 48.052 | 77 | 36 | 1 | 3 | 75 | 56 | 132 | 2.61e-16 | 79.0 |
| MS.gene20924.t1 | AT1G74430 | 72.093 | 43 | 12 | 0 | 29 | 71 | 17 | 59 | 4.30e-16 | 76.3 |
| MS.gene20924.t1 | AT5G61420 | 53.425 | 73 | 30 | 2 | 9 | 77 | 62 | 134 | 7.66e-16 | 77.8 |
| MS.gene20924.t1 | AT4G28110 | 47.619 | 84 | 30 | 2 | 2 | 71 | 45 | 128 | 2.73e-15 | 75.5 |
| MS.gene20924.t1 | AT3G61250 | 36.434 | 129 | 25 | 2 | 1 | 72 | 1 | 129 | 3.05e-15 | 75.5 |
| MS.gene20924.t1 | AT4G05100 | 54.688 | 64 | 25 | 1 | 12 | 71 | 66 | 129 | 3.73e-15 | 75.5 |
| MS.gene20924.t1 | AT5G61420 | 70.213 | 47 | 14 | 0 | 31 | 77 | 9 | 55 | 4.25e-15 | 74.7 |
| MS.gene20924.t1 | AT4G21440 | 53.125 | 64 | 26 | 1 | 12 | 71 | 65 | 128 | 4.26e-15 | 75.5 |
| MS.gene20924.t1 | AT5G07700 | 41.525 | 118 | 50 | 4 | 9 | 107 | 62 | 179 | 6.82e-15 | 74.7 |
| MS.gene20924.t1 | AT5G07700 | 41.525 | 118 | 50 | 4 | 9 | 107 | 62 | 179 | 6.82e-15 | 74.7 |
| MS.gene20924.t1 | AT5G65230 | 48.684 | 76 | 35 | 2 | 3 | 74 | 56 | 131 | 9.46e-15 | 74.3 |
| MS.gene20924.t1 | AT1G18710 | 48.529 | 68 | 31 | 2 | 9 | 72 | 62 | 129 | 1.05e-14 | 73.6 |
| MS.gene20924.t1 | AT1G18570 | 51.515 | 66 | 28 | 1 | 12 | 73 | 66 | 131 | 1.39e-14 | 73.9 |
| MS.gene20924.t1 | AT4G28110 | 70.732 | 41 | 12 | 0 | 31 | 71 | 4 | 44 | 1.49e-14 | 71.6 |
| MS.gene20924.t1 | AT4G34990 | 50.746 | 67 | 29 | 1 | 12 | 74 | 65 | 131 | 2.19e-14 | 72.8 |
| MS.gene20924.t1 | AT5G07690 | 51.282 | 78 | 34 | 2 | 9 | 82 | 62 | 139 | 2.34e-14 | 73.2 |
| MS.gene20924.t1 | AT1G34670 | 50.000 | 72 | 30 | 2 | 9 | 75 | 62 | 132 | 2.53e-14 | 73.6 |
| MS.gene20924.t1 | AT3G12720 | 33.333 | 126 | 26 | 3 | 1 | 69 | 12 | 136 | 2.55e-14 | 72.8 |
| MS.gene20924.t1 | AT5G15310 | 64.000 | 50 | 18 | 0 | 25 | 74 | 3 | 52 | 2.83e-14 | 72.0 |
| MS.gene20924.t1 | AT4G17785 | 52.239 | 67 | 28 | 2 | 9 | 71 | 63 | 129 | 3.11e-14 | 73.2 |
| MS.gene20924.t1 | AT3G13540 | 57.143 | 63 | 24 | 1 | 13 | 72 | 78 | 140 | 3.23e-14 | 71.6 |
| MS.gene20924.t1 | AT4G17785 | 52.239 | 67 | 28 | 2 | 9 | 71 | 63 | 129 | 3.58e-14 | 72.8 |
| MS.gene20924.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 5.08e-14 | 72.4 |
| MS.gene20924.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 5.08e-14 | 72.4 |
| MS.gene20924.t1 | AT5G56110 | 68.889 | 45 | 14 | 0 | 31 | 75 | 88 | 132 | 5.96e-14 | 72.0 |
| MS.gene20924.t1 | AT4G38620 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 6.37e-14 | 71.2 |
| MS.gene20924.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 6.96e-14 | 72.0 |
| MS.gene20924.t1 | AT5G16770 | 49.254 | 67 | 30 | 1 | 12 | 74 | 65 | 131 | 6.96e-14 | 72.0 |
| MS.gene20924.t1 | AT4G22680 | 52.239 | 67 | 28 | 2 | 12 | 74 | 65 | 131 | 7.73e-14 | 70.9 |
| MS.gene20924.t1 | AT2G16720 | 50.746 | 67 | 29 | 1 | 12 | 74 | 65 | 131 | 7.93e-14 | 70.9 |
| MS.gene20924.t1 | AT3G02940 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 8.01e-14 | 71.6 |
| MS.gene20924.t1 | AT3G02940 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 1.13e-13 | 71.2 |
| MS.gene20924.t1 | AT3G02940 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 1.13e-13 | 71.2 |
| MS.gene20924.t1 | AT5G14340 | 39.759 | 83 | 47 | 1 | 31 | 113 | 10 | 89 | 1.49e-13 | 68.6 |
| MS.gene20924.t1 | AT1G22640 | 47.761 | 67 | 31 | 1 | 12 | 74 | 8 | 74 | 2.20e-13 | 68.6 |
| MS.gene20924.t1 | AT4G09460 | 38.318 | 107 | 62 | 1 | 12 | 114 | 65 | 171 | 2.27e-13 | 69.3 |
| MS.gene20924.t1 | AT5G54230 | 46.575 | 73 | 35 | 1 | 3 | 71 | 56 | 128 | 3.92e-13 | 69.7 |
| MS.gene20924.t1 | AT5G14340 | 37.500 | 104 | 59 | 2 | 13 | 113 | 79 | 179 | 4.14e-13 | 68.9 |
| MS.gene20924.t1 | AT5G15310 | 70.455 | 44 | 13 | 0 | 31 | 74 | 88 | 131 | 6.63e-13 | 68.9 |
| MS.gene20924.t1 | AT5G15310 | 70.455 | 44 | 13 | 0 | 31 | 74 | 88 | 131 | 7.36e-13 | 68.9 |
| MS.gene20924.t1 | AT5G15310 | 70.455 | 44 | 13 | 0 | 31 | 74 | 88 | 131 | 7.70e-13 | 67.8 |
| MS.gene20924.t1 | AT4G12350 | 50.000 | 68 | 30 | 2 | 12 | 75 | 65 | 132 | 7.86e-13 | 68.2 |
| MS.gene20924.t1 | AT5G12870 | 55.556 | 54 | 20 | 1 | 12 | 61 | 71 | 124 | 1.71e-12 | 67.4 |
| MS.gene20924.t1 | AT1G22640 | 47.761 | 67 | 31 | 1 | 12 | 74 | 65 | 131 | 1.72e-12 | 67.0 |
| MS.gene20924.t1 | AT5G62320 | 44.595 | 74 | 37 | 1 | 12 | 81 | 73 | 146 | 3.17e-12 | 65.9 |
| MS.gene20924.t1 | AT1G74080 | 49.231 | 65 | 29 | 1 | 12 | 72 | 65 | 129 | 3.65e-12 | 66.6 |
| MS.gene20924.t1 | AT1G63910 | 31.250 | 128 | 31 | 2 | 1 | 71 | 1 | 128 | 6.31e-12 | 66.2 |
| MS.gene20924.t1 | AT3G01140 | 50.794 | 63 | 27 | 2 | 12 | 70 | 65 | 127 | 8.31e-12 | 65.9 |
| MS.gene20924.t1 | AT3G01140 | 50.794 | 63 | 27 | 2 | 12 | 70 | 108 | 170 | 9.62e-12 | 65.9 |
| MS.gene20924.t1 | AT1G22640 | 50.000 | 54 | 27 | 0 | 21 | 74 | 6 | 59 | 1.89e-11 | 62.8 |
| MS.gene20924.t1 | AT1G35515 | 61.364 | 44 | 17 | 0 | 31 | 74 | 88 | 131 | 2.92e-11 | 62.8 |
| MS.gene20924.t1 | AT3G08500 | 56.140 | 57 | 19 | 2 | 9 | 60 | 80 | 135 | 3.25e-11 | 63.9 |
| MS.gene20924.t1 | AT3G28470 | 44.444 | 90 | 43 | 3 | 9 | 91 | 62 | 151 | 5.11e-11 | 63.2 |
Find 52 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATGAAATAAAGAATTTA+TGG | 0.200655 | 1.3:-60812452 | MS.gene20924:CDS |
| ATCATACAGAAGAAGCTAAA+TGG | 0.220741 | 1.3:-60811825 | MS.gene20924:CDS |
| ATTGTTGTTATGTAAGTTTA+AGG | 0.238149 | 1.3:+60811876 | None:intergenic |
| ATTTCCATTGCATATGCTTT+TGG | 0.239502 | 1.3:+60812210 | None:intergenic |
| TTTCAAAAGGTTCAATTCAT+TGG | 0.290203 | 1.3:+60812296 | None:intergenic |
| CAGAAACTCAGAGAAGCTTT+TGG | 0.294526 | 1.3:-60811622 | MS.gene20924:CDS |
| GAGAATAATGGAGAGGAAAA+TGG | 0.306414 | 1.3:-60812357 | MS.gene20924:CDS |
| CATTCAACCCTCAGATTTGA+TGG | 0.319312 | 1.3:-60812148 | MS.gene20924:CDS |
| AACTTTGTACCAGATAGTTT+TGG | 0.328603 | 1.3:-60811718 | MS.gene20924:CDS |
| CTACTAGTGTTTGAATCATT+TGG | 0.336320 | 1.3:+60812075 | None:intergenic |
| TCAGAAGCAAGCATTAAGAT+AGG | 0.337065 | 1.3:+60811775 | None:intergenic |
| TCAAATTGCGGCGCAATTGC+CGG | 0.343894 | 1.3:-60812484 | MS.gene20924:CDS |
| TTCTTCTTGGGGAGGATTGA+TGG | 0.346985 | 1.3:-60811851 | MS.gene20924:CDS |
| AAGAAGAGACTGAGACAAAA+AGG | 0.366324 | 1.3:-60812417 | MS.gene20924:CDS |
| AAAATAGCTCCAAAACTATC+TGG | 0.372468 | 1.3:+60811709 | None:intergenic |
| AACCATTGGTTCAACCAAAC+TGG | 0.373334 | 1.3:-60812051 | MS.gene20924:CDS |
| CAAATTGCGGCGCAATTGCC+GGG | 0.376424 | 1.3:-60812483 | MS.gene20924:CDS |
| AAAAGCATATGCAATGGAAA+TGG | 0.406668 | 1.3:-60812208 | MS.gene20924:CDS |
| TCTGAGTTTCTGAATGTCCT+TGG | 0.406727 | 1.3:+60811633 | None:intergenic |
| TTGTCTGAGATTGAGAATAA+TGG | 0.407472 | 1.3:-60812369 | MS.gene20924:CDS |
| TCTTGTTCTTGCTTTGTATG+AGG | 0.413126 | 1.3:+60811685 | None:intergenic |
| TTGAACTCTCTGATTTCAAA+AGG | 0.423755 | 1.3:+60812283 | None:intergenic |
| TCATACAGAAGAAGCTAAAT+GGG | 0.442713 | 1.3:-60811824 | MS.gene20924:CDS |
| GTTGTTACAAGCAGAAGCTT+AGG | 0.455012 | 1.3:-60812999 | MS.gene20924:CDS |
| TTCCAGTTTGGTTGAACCAA+TGG | 0.463181 | 1.3:+60812049 | None:intergenic |
| CATGGTTGTTGATGTTGTTG+TGG | 0.467386 | 1.3:+60811987 | None:intergenic |
| TCATTGGATAATAATATCTC+TGG | 0.476615 | 1.3:+60812312 | None:intergenic |
| ATGGTTGTTGATGTTGTTGT+GGG | 0.482724 | 1.3:+60811988 | None:intergenic |
| TTTGTATAACTCATTTGAAG+TGG | 0.483418 | 1.3:+60812117 | None:intergenic |
| TTATTCTCAATCTCAGACAA+TGG | 0.501529 | 1.3:+60812372 | None:intergenic |
| CAGAAGCAAGCATTAAGATA+GGG | 0.504178 | 1.3:+60811776 | None:intergenic |
| TCTGGTACAAAGTTCATCAA+TGG | 0.513100 | 1.3:+60811727 | None:intergenic |
| ATTTCATTGTCTGTCCTCCC+CGG | 0.513376 | 1.3:+60812465 | None:intergenic |
| GACAATGGTTTATGAGTCAC+AGG | 0.514909 | 1.3:+60812387 | None:intergenic |
| AGAAGCTTAGGAAAGGTCTA+TGG | 0.519654 | 1.3:-60812987 | MS.gene20924:CDS |
| AAAATTTCCCATCAAATCTG+AGG | 0.537811 | 1.3:+60812140 | None:intergenic |
| ATTCAACCCTCAGATTTGAT+GGG | 0.538604 | 1.3:-60812147 | MS.gene20924:CDS |
| TTCACCAAAAGCATATGCAA+TGG | 0.574736 | 1.3:-60812214 | MS.gene20924:CDS |
| TATCAGATGGTCTCAAATTG+CGG | 0.579070 | 1.3:-60812496 | MS.gene20924:intron |
| AGGAAAATTCACAAATGGGA+AGG | 0.581895 | 1.3:-60813029 | None:intergenic |
| TTGCGGCGCAATTGCCGGGG+AGG | 0.583739 | 1.3:-60812479 | MS.gene20924:CDS |
| CTCTGTGGAAACATTGTCAG+AGG | 0.587422 | 1.3:+60811921 | None:intergenic |
| AAATTTCCCATCAAATCTGA+GGG | 0.588053 | 1.3:+60812141 | None:intergenic |
| CAAACACTAGTAGTAACCAT+TGG | 0.594685 | 1.3:-60812065 | MS.gene20924:CDS |
| GCATATGCAATGGAAATGGA+AGG | 0.607612 | 1.3:-60812204 | MS.gene20924:CDS |
| TACAAGCAGAAGCTTAGGAA+AGG | 0.614815 | 1.3:-60812994 | MS.gene20924:CDS |
| GGTACAAAGTTCATCAATGG+AGG | 0.632864 | 1.3:+60811730 | None:intergenic |
| GCTGCTAGTTCTCATCTCTG+TGG | 0.644097 | 1.3:+60811906 | None:intergenic |
| AAATTGCGGCGCAATTGCCG+GGG | 0.649843 | 1.3:-60812482 | MS.gene20924:CDS |
| TGAGATTGAGAATAATGGAG+AGG | 0.653412 | 1.3:-60812364 | MS.gene20924:CDS |
| TCTGTGGAAACATTGTCAGA+GGG | 0.661164 | 1.3:+60811922 | None:intergenic |
| AAAAGAATTAGGAAGAAACA+TGG | 0.684494 | 1.3:+60811969 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTGTTTATTATTATC+AGG | - | chr1.3:60811828-60811847 | MS.gene20924:CDS | 10.0% |
| !! | ACAATGAAATAAAGAATTTA+TGG | - | chr1.3:60812175-60812194 | MS.gene20924:CDS | 15.0% |
| !! | ACATTATTATTATATGCTAA+TGG | + | chr1.3:60811769-60811788 | None:intergenic | 15.0% |
| !! | CATAGGAGAAAATAAAAAAA+AGG | + | chr1.3:60812007-60812026 | None:intergenic | 20.0% |
| !!! | AACTTCTAATATATTTTCCA+AGG | - | chr1.3:60812977-60812996 | MS.gene20924:CDS | 20.0% |
| !!! | ATAACAACAATTTTTCTTCT+TGG | - | chr1.3:60812763-60812782 | MS.gene20924:intron | 20.0% |
| !!! | ATTGTTGTTATGTAAGTTTA+AGG | + | chr1.3:60812754-60812773 | None:intergenic | 20.0% |
| !!! | CTTTTTTTTATTTTCTCCTA+TGG | - | chr1.3:60812005-60812024 | MS.gene20924:CDS | 20.0% |
| !!! | TAACAACAATTTTTCTTCTT+GGG | - | chr1.3:60812764-60812783 | MS.gene20924:intron | 20.0% |
| ! | AAAAGAATTAGGAAGAAACA+TGG | + | chr1.3:60812661-60812680 | None:intergenic | 25.0% |
| ! | TCATTGGATAATAATATCTC+TGG | + | chr1.3:60812318-60812337 | None:intergenic | 25.0% |
| ! | TTAAGACCTGATTTAAAAAG+AGG | - | chr1.3:60811897-60811916 | MS.gene20924:CDS | 25.0% |
| ! | TTTCAAAAGGTTCAATTCAT+TGG | + | chr1.3:60812334-60812353 | None:intergenic | 25.0% |
| ! | TTTGTATAACTCATTTGAAG+TGG | + | chr1.3:60812513-60812532 | None:intergenic | 25.0% |
| !! | AACAACAATTTTTCTTCTTG+GGG | - | chr1.3:60812765-60812784 | MS.gene20924:intron | 25.0% |
| !! | AATGTTCCTCTTTTTAAATC+AGG | + | chr1.3:60811906-60811925 | None:intergenic | 25.0% |
| !! | ACAAAGAGTTTTTCATAGAT+AGG | - | chr1.3:60812454-60812473 | MS.gene20924:CDS | 25.0% |
| !! | TATCAAAAGATTTTCCAGTT+TGG | + | chr1.3:60812593-60812612 | None:intergenic | 25.0% |
| !! | TTCATTTTCTTCTTATCAGA+TGG | - | chr1.3:60812118-60812137 | MS.gene20924:CDS | 25.0% |
| AAAATAGCTCCAAAACTATC+TGG | + | chr1.3:60812921-60812940 | None:intergenic | 30.0% | |
| AAAATTTCCCATCAAATCTG+AGG | + | chr1.3:60812490-60812509 | None:intergenic | 30.0% | |
| AAATTTCCCATCAAATCTGA+GGG | + | chr1.3:60812489-60812508 | None:intergenic | 30.0% | |
| AAGAAGATGAAAAACTACTG+AGG | - | chr1.3:60811670-60811689 | MS.gene20924:CDS | 30.0% | |
| ATCATACAGAAGAAGCTAAA+TGG | - | chr1.3:60812802-60812821 | MS.gene20924:intron | 30.0% | |
| CATATAACAAAGTATGGTCA+TGG | - | chr1.3:60811693-60811712 | MS.gene20924:CDS | 30.0% | |
| CTACTAGTGTTTGAATCATT+TGG | + | chr1.3:60812555-60812574 | None:intergenic | 30.0% | |
| TCATACAGAAGAAGCTAAAT+GGG | - | chr1.3:60812803-60812822 | MS.gene20924:intron | 30.0% | |
| TTATTCTCAATCTCAGACAA+TGG | + | chr1.3:60812258-60812277 | None:intergenic | 30.0% | |
| TTGAACTCTCTGATTTCAAA+AGG | + | chr1.3:60812347-60812366 | None:intergenic | 30.0% | |
| TTGTTGCAGCAAAAAGAATT+AGG | + | chr1.3:60812672-60812691 | None:intergenic | 30.0% | |
| ! | AAAAGCATATGCAATGGAAA+TGG | - | chr1.3:60812419-60812438 | MS.gene20924:CDS | 30.0% |
| ! | AACTTTGTACCAGATAGTTT+TGG | - | chr1.3:60812909-60812928 | MS.gene20924:intron | 30.0% |
| ! | AGTAGTTTTTCATCTTCTTC+TGG | + | chr1.3:60811669-60811688 | None:intergenic | 30.0% |
| ! | ATTTCCATTGCATATGCTTT+TGG | + | chr1.3:60812420-60812439 | None:intergenic | 30.0% |
| ! | TTGTCTGAGATTGAGAATAA+TGG | - | chr1.3:60812258-60812277 | MS.gene20924:CDS | 30.0% |
| AAGAAGAGACTGAGACAAAA+AGG | - | chr1.3:60812210-60812229 | MS.gene20924:CDS | 35.0% | |
| ATGGTTAAGCAAGAGTGATA+AGG | - | chr1.3:60812024-60812043 | MS.gene20924:CDS | 35.0% | |
| ATGGTTGTTGATGTTGTTGT+GGG | + | chr1.3:60812642-60812661 | None:intergenic | 35.0% | |
| ATTCAACCCTCAGATTTGAT+GGG | - | chr1.3:60812480-60812499 | MS.gene20924:CDS | 35.0% | |
| CAAACACTAGTAGTAACCAT+TGG | - | chr1.3:60812562-60812581 | MS.gene20924:intron | 35.0% | |
| CAGAAGCAAGCATTAAGATA+GGG | + | chr1.3:60812854-60812873 | None:intergenic | 35.0% | |
| CTGAGGCATATAACAAAGTA+TGG | - | chr1.3:60811687-60811706 | MS.gene20924:CDS | 35.0% | |
| GATAATTTACCTGCTTGCTT+AGG | + | chr1.3:60811735-60811754 | None:intergenic | 35.0% | |
| TATCACTCTTGCTTAACCAT+AGG | + | chr1.3:60812024-60812043 | None:intergenic | 35.0% | |
| TATCAGATGGTCTCAAATTG+CGG | - | chr1.3:60812131-60812150 | MS.gene20924:CDS | 35.0% | |
| TCAGAAGCAAGCATTAAGAT+AGG | + | chr1.3:60812855-60812874 | None:intergenic | 35.0% | |
| TCTGGTACAAAGTTCATCAA+TGG | + | chr1.3:60812903-60812922 | None:intergenic | 35.0% | |
| TCTTGTTCTTGCTTTGTATG+AGG | + | chr1.3:60812945-60812964 | None:intergenic | 35.0% | |
| TTATCAGGTTTGCAAAGATG+TGG | - | chr1.3:60811843-60811862 | MS.gene20924:CDS | 35.0% | |
| TTGAACTTCATGCAGTACTA+GGG | - | chr1.3:60811952-60811971 | MS.gene20924:CDS | 35.0% | |
| ! | AACAATTTTTCTTCTTGGGG+AGG | - | chr1.3:60812768-60812787 | MS.gene20924:intron | 35.0% |
| ! | GAGAATAATGGAGAGGAAAA+TGG | - | chr1.3:60812270-60812289 | MS.gene20924:CDS | 35.0% |
| ! | TGAGATTGAGAATAATGGAG+AGG | - | chr1.3:60812263-60812282 | MS.gene20924:CDS | 35.0% |
| ! | TTCACCAAAAGCATATGCAA+TGG | - | chr1.3:60812413-60812432 | MS.gene20924:CDS | 35.0% |
| AAGAGGAACATTCTCACAAG+AGG | - | chr1.3:60811914-60811933 | MS.gene20924:CDS | 40.0% | |
| AGAAGCTTAGGAAAGGTCTA+TGG | - | chr1.3:60811640-60811659 | MS.gene20924:CDS | 40.0% | |
| CAAAGTATGGTCATGGATGT+TGG | - | chr1.3:60811700-60811719 | MS.gene20924:CDS | 40.0% | |
| CATGGTTGTTGATGTTGTTG+TGG | + | chr1.3:60812643-60812662 | None:intergenic | 40.0% | |
| CATTCAACCCTCAGATTTGA+TGG | - | chr1.3:60812479-60812498 | MS.gene20924:CDS | 40.0% | |
| GACAATGGTTTATGAGTCAC+AGG | + | chr1.3:60812243-60812262 | None:intergenic | 40.0% | |
| GCATATGCAATGGAAATGGA+AGG | - | chr1.3:60812423-60812442 | MS.gene20924:CDS | 40.0% | |
| GGTACAAAGTTCATCAATGG+AGG | + | chr1.3:60812900-60812919 | None:intergenic | 40.0% | |
| GTTAAGCAAGAGTGATAAGG+AGG | - | chr1.3:60812027-60812046 | MS.gene20924:CDS | 40.0% | |
| GTTGAACTTCATGCAGTACT+AGG | - | chr1.3:60811951-60811970 | MS.gene20924:CDS | 40.0% | |
| GTTGTTACAAGCAGAAGCTT+AGG | - | chr1.3:60811628-60811647 | MS.gene20924:CDS | 40.0% | |
| TACAAGCAGAAGCTTAGGAA+AGG | - | chr1.3:60811633-60811652 | MS.gene20924:CDS | 40.0% | |
| TCTGAGTTTCTGAATGTCCT+TGG | + | chr1.3:60812997-60813016 | None:intergenic | 40.0% | |
| TCTGTGGAAACATTGTCAGA+GGG | + | chr1.3:60812708-60812727 | None:intergenic | 40.0% | |
| TTCATGCAGTACTAGGGAAT+AGG | - | chr1.3:60811958-60811977 | MS.gene20924:CDS | 40.0% | |
| TTCCAGTTTGGTTGAACCAA+TGG | + | chr1.3:60812581-60812600 | None:intergenic | 40.0% | |
| ! | CAGAAACTCAGAGAAGCTTT+TGG | - | chr1.3:60813005-60813024 | MS.gene20924:CDS | 40.0% |
| !! | AACCATTGGTTCAACCAAAC+TGG | - | chr1.3:60812576-60812595 | MS.gene20924:intron | 40.0% |
| AAAGATGTGGTAAGAGCTGC+AGG | - | chr1.3:60811856-60811875 | MS.gene20924:CDS | 45.0% | |
| AAGAGTGATAAGGAGGTCGA+GGG | - | chr1.3:60812034-60812053 | MS.gene20924:CDS | 45.0% | |
| AGTTCAGTACCTAAGCAAGC+AGG | - | chr1.3:60811723-60811742 | MS.gene20924:CDS | 45.0% | |
| ATTTCATTGTCTGTCCTCCC+CGG | + | chr1.3:60812165-60812184 | None:intergenic | 45.0% | |
| CTCTGTGGAAACATTGTCAG+AGG | + | chr1.3:60812709-60812728 | None:intergenic | 45.0% | |
| TTCTTCTTGGGGAGGATTGA+TGG | - | chr1.3:60812776-60812795 | MS.gene20924:intron | 45.0% | |
| CAAGAGTGATAAGGAGGTCG+AGG | - | chr1.3:60812033-60812052 | MS.gene20924:CDS | 50.0% | |
| TCAAATTGCGGCGCAATTGC+CGG | - | chr1.3:60812143-60812162 | MS.gene20924:CDS | 50.0% | |
| ! | GCTGCTAGTTCTCATCTCTG+TGG | + | chr1.3:60812724-60812743 | None:intergenic | 50.0% |
| AAATTGCGGCGCAATTGCCG+GGG | - | chr1.3:60812145-60812164 | MS.gene20924:CDS | 55.0% | |
| CAAATTGCGGCGCAATTGCC+GGG | - | chr1.3:60812144-60812163 | MS.gene20924:CDS | 55.0% | |
| GTAAGAGCTGCAGGCTTAGG+TGG | - | chr1.3:60811865-60811884 | MS.gene20924:CDS | 55.0% | |
| GTGGTAAGAGCTGCAGGCTT+AGG | - | chr1.3:60811862-60811881 | MS.gene20924:CDS | 55.0% | |
| CGAGGGCTCGATCTCACTCT+CGG | - | chr1.3:60812051-60812070 | MS.gene20924:CDS | 60.0% | |
| TTGCGGCGCAATTGCCGGGG+AGG | - | chr1.3:60812148-60812167 | MS.gene20924:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 60811612 | 60813037 | 60811612 | ID=MS.gene20924 |
| chr1.3 | mRNA | 60811612 | 60813037 | 60811612 | ID=MS.gene20924.t1;Parent=MS.gene20924 |
| chr1.3 | exon | 60812946 | 60813037 | 60812946 | ID=MS.gene20924.t1.exon1;Parent=MS.gene20924.t1 |
| chr1.3 | CDS | 60812946 | 60813037 | 60812946 | ID=cds.MS.gene20924.t1;Parent=MS.gene20924.t1 |
| chr1.3 | exon | 60811612 | 60812512 | 60811612 | ID=MS.gene20924.t1.exon2;Parent=MS.gene20924.t1 |
| chr1.3 | CDS | 60811612 | 60812512 | 60811612 | ID=cds.MS.gene20924.t1;Parent=MS.gene20924.t1 |
| Gene Sequence |
| Protein sequence |
