Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21018.t1 | AES61472.2 | 98.2 | 227 | 4 | 0 | 2 | 228 | 42 | 268 | 4.30E-128 | 467.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21018.t1 | A2Y007 | 61.1 | 229 | 78 | 3 | 2 | 226 | 78 | 299 | 8.3e-72 | 271.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21018.t1 | A0A072VNV2 | 98.2 | 227 | 4 | 0 | 2 | 228 | 42 | 268 | 3.1e-128 | 467.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene21018.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene21018 | 0.850152 | 2.04E-60 | -1.69E-46 |
| MS.gene049711 | MS.gene21018 | 0.803651 | 3.15E-49 | -1.69E-46 |
| MS.gene049807 | MS.gene21018 | 0.80902 | 2.33E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene21018 | 0.804314 | 2.29E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene21018 | 0.813935 | 1.99E-51 | -1.69E-46 |
| MS.gene052150 | MS.gene21018 | 0.811543 | 6.64E-51 | -1.69E-46 |
| MS.gene052609 | MS.gene21018 | 0.811047 | 8.51E-51 | -1.69E-46 |
| MS.gene053143 | MS.gene21018 | 0.815162 | 1.06E-51 | -1.69E-46 |
| MS.gene053563 | MS.gene21018 | 0.812888 | 3.38E-51 | -1.69E-46 |
| MS.gene053581 | MS.gene21018 | 0.836018 | 1.20E-56 | -1.69E-46 |
| MS.gene055144 | MS.gene21018 | 0.807118 | 5.91E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene21018 | 0.800992 | 1.11E-48 | -1.69E-46 |
| MS.gene055469 | MS.gene21018 | 0.814417 | 1.56E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene21018 | 0.814235 | 1.71E-51 | -1.69E-46 |
| MS.gene056005 | MS.gene21018 | 0.926702 | 3.26E-91 | -1.69E-46 |
| MS.gene056308 | MS.gene21018 | 0.809516 | 1.82E-50 | -1.69E-46 |
| MS.gene058668 | MS.gene21018 | 0.816512 | 5.32E-52 | -1.69E-46 |
| MS.gene058852 | MS.gene21018 | 0.80202 | 6.84E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21018.t1 | MTR_1g084060 | 98.238 | 227 | 4 | 0 | 2 | 228 | 42 | 268 | 1.07e-169 | 468 |
| MS.gene21018.t1 | MTR_1g084060 | 98.238 | 227 | 4 | 0 | 2 | 228 | 42 | 268 | 1.99e-169 | 469 |
| MS.gene21018.t1 | MTR_5g033720 | 53.778 | 225 | 88 | 4 | 2 | 220 | 95 | 309 | 1.56e-69 | 216 |
| MS.gene21018.t1 | MTR_1g017080 | 48.472 | 229 | 105 | 4 | 2 | 225 | 142 | 362 | 3.09e-60 | 194 |
| MS.gene21018.t1 | MTR_5g085860 | 48.899 | 227 | 91 | 4 | 2 | 220 | 69 | 278 | 5.71e-58 | 186 |
| MS.gene21018.t1 | MTR_2g024390 | 50.909 | 220 | 94 | 4 | 2 | 217 | 140 | 349 | 1.25e-56 | 184 |
| MS.gene21018.t1 | MTR_1g012960 | 38.265 | 196 | 94 | 6 | 30 | 205 | 232 | 420 | 8.66e-31 | 117 |
| MS.gene21018.t1 | MTR_3g106400 | 38.614 | 202 | 86 | 6 | 30 | 205 | 166 | 355 | 9.17e-31 | 117 |
| MS.gene21018.t1 | MTR_3g106400 | 38.614 | 202 | 86 | 6 | 30 | 205 | 166 | 355 | 9.17e-31 | 116 |
| MS.gene21018.t1 | MTR_3g106400 | 38.614 | 202 | 86 | 6 | 30 | 205 | 166 | 355 | 2.39e-30 | 116 |
| MS.gene21018.t1 | MTR_2g461240 | 36.788 | 193 | 104 | 4 | 28 | 205 | 150 | 339 | 7.28e-30 | 114 |
| MS.gene21018.t1 | MTR_4g116545 | 35.263 | 190 | 109 | 2 | 30 | 205 | 126 | 315 | 3.97e-29 | 112 |
| MS.gene21018.t1 | MTR_4g116545 | 38.021 | 192 | 101 | 5 | 30 | 205 | 126 | 315 | 4.01e-29 | 111 |
| MS.gene21018.t1 | MTR_5g011070 | 34.555 | 191 | 110 | 3 | 30 | 205 | 86 | 276 | 6.80e-27 | 105 |
| MS.gene21018.t1 | MTR_7g065050 | 38.356 | 73 | 43 | 1 | 135 | 207 | 296 | 366 | 3.80e-11 | 62.4 |
| MS.gene21018.t1 | MTR_4g019450 | 45.000 | 60 | 33 | 0 | 148 | 207 | 362 | 421 | 4.05e-11 | 62.4 |
| MS.gene21018.t1 | MTR_3g112290 | 42.466 | 73 | 39 | 1 | 135 | 207 | 340 | 409 | 7.18e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21018.t1 | AT1G23380 | 50.000 | 228 | 94 | 4 | 2 | 222 | 110 | 324 | 1.09e-69 | 216 |
| MS.gene21018.t1 | AT1G23380 | 50.000 | 228 | 94 | 4 | 2 | 222 | 108 | 322 | 1.25e-69 | 216 |
| MS.gene21018.t1 | AT1G70510 | 49.333 | 225 | 100 | 3 | 2 | 222 | 92 | 306 | 1.95e-67 | 210 |
| MS.gene21018.t1 | AT1G70510 | 48.472 | 229 | 100 | 4 | 2 | 222 | 92 | 310 | 4.24e-65 | 204 |
| MS.gene21018.t1 | AT1G62360 | 50.446 | 224 | 95 | 4 | 2 | 219 | 144 | 357 | 1.05e-58 | 190 |
| MS.gene21018.t1 | AT4G08150 | 47.111 | 225 | 105 | 5 | 2 | 219 | 157 | 374 | 3.47e-57 | 186 |
| MS.gene21018.t1 | AT5G25220 | 38.342 | 193 | 98 | 4 | 28 | 205 | 217 | 403 | 4.70e-32 | 121 |
| MS.gene21018.t1 | AT5G25220 | 38.342 | 193 | 98 | 4 | 28 | 205 | 217 | 403 | 6.08e-32 | 120 |
| MS.gene21018.t1 | AT5G11060 | 38.889 | 198 | 88 | 6 | 29 | 205 | 182 | 367 | 6.51e-32 | 120 |
| MS.gene21018.t1 | AT4G32040 | 37.113 | 194 | 104 | 4 | 28 | 205 | 171 | 362 | 9.29e-31 | 117 |
| MS.gene21018.t1 | AT4G32040 | 37.113 | 194 | 104 | 4 | 28 | 205 | 171 | 362 | 9.75e-31 | 117 |
| MS.gene21018.t1 | AT1G62990 | 33.333 | 225 | 128 | 6 | 2 | 205 | 52 | 275 | 3.34e-29 | 111 |
| MS.gene21018.t1 | AT1G75430 | 49.091 | 55 | 28 | 0 | 153 | 207 | 209 | 263 | 2.72e-11 | 62.4 |
| MS.gene21018.t1 | AT5G02030 | 46.667 | 60 | 32 | 0 | 148 | 207 | 350 | 409 | 4.34e-11 | 62.4 |
| MS.gene21018.t1 | AT1G19700 | 45.000 | 60 | 33 | 0 | 148 | 207 | 353 | 412 | 4.83e-11 | 62.4 |
| MS.gene21018.t1 | AT1G19700 | 45.000 | 60 | 33 | 0 | 148 | 207 | 353 | 412 | 4.83e-11 | 62.4 |
| MS.gene21018.t1 | AT1G19700 | 45.000 | 60 | 33 | 0 | 148 | 207 | 353 | 412 | 4.83e-11 | 62.4 |
| MS.gene21018.t1 | AT1G19700 | 45.000 | 60 | 33 | 0 | 148 | 207 | 353 | 412 | 4.83e-11 | 62.4 |
| MS.gene21018.t1 | AT1G19700 | 45.000 | 60 | 33 | 0 | 148 | 207 | 353 | 412 | 4.83e-11 | 62.4 |
| MS.gene21018.t1 | AT1G75410 | 46.667 | 60 | 32 | 0 | 148 | 207 | 348 | 407 | 6.22e-11 | 62.0 |
| MS.gene21018.t1 | AT1G75410 | 46.667 | 60 | 32 | 0 | 148 | 207 | 348 | 407 | 6.22e-11 | 62.0 |
Find 54 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCTCTTCTTGAAGAAAT+AGG | 0.173162 | 1.3:-59805137 | MS.gene21018:CDS |
| GAAATGCTCCTTCGTAAGTA+TGG | 0.209222 | 1.3:-59796478 | MS.gene21018:CDS |
| TCTCACGGAAAGCATTATTT+TGG | 0.227729 | 1.3:+59805102 | None:intergenic |
| ATTTCTCAAGAAAAGGAAAA+AGG | 0.267954 | 1.3:-59796428 | MS.gene21018:CDS |
| CTTCTCTTCTTGAAGAAATA+GGG | 0.308161 | 1.3:-59805136 | MS.gene21018:CDS |
| ATATAAAGCAGATAAATAAT+TGG | 0.326164 | 1.3:-59795755 | MS.gene21018:CDS |
| TTTACCTGAACGGTTGTAAT+CGG | 0.376328 | 1.3:+59804757 | None:intergenic |
| TGTTGCTTCATTGAAGGGCT+TGG | 0.378477 | 1.3:+59804844 | None:intergenic |
| AATAATGCTTTCCGTGAGAT+AGG | 0.392343 | 1.3:-59805098 | MS.gene21018:CDS |
| TCCTGACCTTGACCACTTCA+TGG | 0.397547 | 1.3:-59805069 | MS.gene21018:intron |
| AAATAATGTTGCTTCATTGA+AGG | 0.405931 | 1.3:+59804838 | None:intergenic |
| ATTTGTATTACCGTGGGATA+AGG | 0.406677 | 1.3:+59796343 | None:intergenic |
| GACAGGAATTTCTCAAGAAA+AGG | 0.410633 | 1.3:-59796435 | MS.gene21018:CDS |
| AATAATGTTGCTTCATTGAA+GGG | 0.423656 | 1.3:+59804839 | None:intergenic |
| GGATGCAAGAAAAGCATTGA+TGG | 0.424199 | 1.3:-59796392 | MS.gene21018:CDS |
| GGAAAGGTTGAAGCAGTAGA+AGG | 0.448898 | 1.3:-59796553 | MS.gene21018:CDS |
| ATTGGTGGAACGTCCACTAT+AGG | 0.452627 | 1.3:-59796369 | MS.gene21018:CDS |
| TCATCCGATTACAACCGTTC+AGG | 0.455239 | 1.3:-59804761 | MS.gene21018:intron |
| GGGTCAGATCATGAGGCAGC+AGG | 0.476443 | 1.3:-59796601 | MS.gene21018:intron |
| TTACAGAGGTCCTGCTATGC+TGG | 0.477108 | 1.3:+59795656 | None:intergenic |
| ACCATGAAGTGGTCAAGGTC+AGG | 0.484448 | 1.3:+59805068 | None:intergenic |
| TGGGATAAGGCCACCTATAG+TGG | 0.485346 | 1.3:+59796356 | None:intergenic |
| AAATGCTCCTTCGTAAGTAT+GGG | 0.496084 | 1.3:-59796477 | MS.gene21018:CDS |
| ATTCCTGTCTTAAGTTGCTA+AGG | 0.503658 | 1.3:+59796449 | None:intergenic |
| CAAGAAAAGCATTGATGGAT+TGG | 0.506407 | 1.3:-59796387 | MS.gene21018:CDS |
| GTAAGTAGCACCAGCATAGC+AGG | 0.513299 | 1.3:-59795666 | MS.gene21018:CDS |
| AGAAGAGAAGCAAGTTCTGT+TGG | 0.544757 | 1.3:+59805149 | None:intergenic |
| TCTTTAAGCTCTTTATCACC+TGG | 0.551142 | 1.3:+59796499 | None:intergenic |
| GAAAAGCATTGATGGATTGG+TGG | 0.555034 | 1.3:-59796384 | MS.gene21018:CDS |
| TTACCTTAGCAACTTAAGAC+AGG | 0.555596 | 1.3:-59796452 | MS.gene21018:CDS |
| ACAACTAAACGAACTTTGCA+AGG | 0.560050 | 1.3:-59804798 | MS.gene21018:CDS |
| GAAAATCTCATATCTTCAGA+TGG | 0.560173 | 1.3:+59795702 | None:intergenic |
| CTGCAGCTGTCAGATATGAC+TGG | 0.564508 | 1.3:-59795783 | MS.gene21018:CDS |
| TTAAGTTCTCTTTACCTGAA+CGG | 0.565840 | 1.3:+59804747 | None:intergenic |
| TCAGAGGATGAAATGAGTTG+TGG | 0.568316 | 1.3:-59796574 | MS.gene21018:CDS |
| AATATACCATGAAGTGGTCA+AGG | 0.574865 | 1.3:+59805063 | None:intergenic |
| TAAAAGAATATACCATGAAG+TGG | 0.580028 | 1.3:+59805057 | None:intergenic |
| TCAATCAGAGGAAAAGACAC+TGG | 0.584588 | 1.3:-59795728 | MS.gene21018:CDS |
| CAGGATCATCTCCTATCTCA+CGG | 0.597806 | 1.3:+59805087 | None:intergenic |
| CTTTGTGGCACATCATGTCC+AGG | 0.602003 | 1.3:-59796517 | MS.gene21018:CDS |
| GGTGGTCATGATGAACTTTG+TGG | 0.607949 | 1.3:-59796532 | MS.gene21018:CDS |
| AATGCTCCTTCGTAAGTATG+GGG | 0.615416 | 1.3:-59796476 | MS.gene21018:CDS |
| GGTCAGATCATGAGGCAGCA+GGG | 0.615533 | 1.3:-59796600 | MS.gene21018:intron |
| CTATAGGTGGCCTTATCCCA+CGG | 0.616947 | 1.3:-59796353 | MS.gene21018:intron |
| CAACTAAACGAACTTTGCAA+GGG | 0.626350 | 1.3:-59804797 | MS.gene21018:CDS |
| AACTAAACGAACTTTGCAAG+GGG | 0.629556 | 1.3:-59804796 | MS.gene21018:CDS |
| ATAATTGGTTCATCAATCAG+AGG | 0.632866 | 1.3:-59795740 | MS.gene21018:CDS |
| AGTTCTTCATAGATACAAAG+AGG | 0.649236 | 1.3:-59804873 | MS.gene21018:CDS |
| GGTGGAACGTCCACTATAGG+TGG | 0.655633 | 1.3:-59796366 | MS.gene21018:CDS |
| GGATGAAATGAGTTGTGGAA+AGG | 0.661174 | 1.3:-59796569 | MS.gene21018:CDS |
| TGAGGCAGCAGGGACATCAG+AGG | 0.661669 | 1.3:-59796590 | MS.gene21018:CDS |
| AAGGTTGAAGCAGTAGAAGG+TGG | 0.681013 | 1.3:-59796550 | MS.gene21018:CDS |
| ATGCTCCTTCGTAAGTATGG+GGG | 0.702025 | 1.3:-59796475 | MS.gene21018:CDS |
| CCTGCAGAATAAATTTACAG+AGG | 0.707916 | 1.3:+59795642 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 59795656 | 59805345 | 59795656 | ID=MS.gene21018 |
| chr1.3 | mRNA | 59795656 | 59805345 | 59795656 | ID=MS.gene21018.t1;Parent=MS.gene21018 |
| chr1.3 | exon | 59805343 | 59805345 | 59805343 | ID=MS.gene21018.t1.exon1;Parent=MS.gene21018.t1 |
| chr1.3 | CDS | 59805343 | 59805345 | 59805343 | ID=cds.MS.gene21018.t1;Parent=MS.gene21018.t1 |
| chr1.3 | exon | 59805070 | 59805180 | 59805070 | ID=MS.gene21018.t1.exon2;Parent=MS.gene21018.t1 |
| chr1.3 | CDS | 59805070 | 59805180 | 59805070 | ID=cds.MS.gene21018.t1;Parent=MS.gene21018.t1 |
| chr1.3 | exon | 59804762 | 59804909 | 59804762 | ID=MS.gene21018.t1.exon3;Parent=MS.gene21018.t1 |
| chr1.3 | CDS | 59804762 | 59804909 | 59804762 | ID=cds.MS.gene21018.t1;Parent=MS.gene21018.t1 |
| chr1.3 | exon | 59796354 | 59796616 | 59796354 | ID=MS.gene21018.t1.exon4;Parent=MS.gene21018.t1 |
| chr1.3 | CDS | 59796354 | 59796616 | 59796354 | ID=cds.MS.gene21018.t1;Parent=MS.gene21018.t1 |
| chr1.3 | exon | 59795656 | 59795817 | 59795656 | ID=MS.gene21018.t1.exon5;Parent=MS.gene21018.t1 |
| chr1.3 | CDS | 59795656 | 59795817 | 59795656 | ID=cds.MS.gene21018.t1;Parent=MS.gene21018.t1 |
| Gene Sequence |
| Protein sequence |
