AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21685

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21685.t1 MTR_5g008890 99.444 180 1 0 1 180 1 180 6.34e-132 367
MS.gene21685.t1 MTR_3g054060 48.276 174 52 5 1 171 31 169 8.36e-44 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21685.t1 AT1G51510 64.246 179 54 6 9 178 7 184 5.07e-74 221
MS.gene21685.t1 AT5G64200 32.353 102 68 1 79 180 5 105 5.17e-11 60.5
MS.gene21685.t1 AT5G64200 32.353 102 68 1 79 180 5 105 5.17e-11 60.5

Find 43 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAACCATTTATGTTGAT+TGG 0.221624 5.2:+2659944 MS.gene21685:CDS
TATTGAAGGATGGATTATTT+TGG 0.283878 5.2:+2657525 MS.gene21685:CDS
AACTCTCGTCTCGCTGATTC+CGG 0.283971 5.2:+2657367 MS.gene21685:CDS
GAAAGCCCAATCAACATAAA+TGG 0.296555 5.2:-2659950 None:intergenic
CGCCGCCGACGGAGAAGCTT+TGG 0.306556 5.2:+2657189 MS.gene21685:CDS
AGTGATGGCGGATTTGAGTT+TGG 0.318147 5.2:-2657284 None:intergenic
GATGATTTGCAGAATGCTTT+TGG 0.361063 5.2:+2657577 MS.gene21685:CDS
GTGATGGCGGATTTGAGTTT+GGG 0.363430 5.2:-2657283 None:intergenic
CTGACGGAAACCGCGACCTT+TGG 0.364553 5.2:-2657323 None:intergenic
CTTCTAACTGACTCATTGAT+AGG 0.375297 5.2:-2659982 None:intergenic
ACAAACCATTTATGTTGATT+GGG 0.381801 5.2:+2659945 MS.gene21685:CDS
CTCACTGCTGAGGGTGGTCC+TGG 0.420911 5.2:+2657400 MS.gene21685:CDS
GCCAGAGGAAGATGACTTGA+TGG 0.429124 5.2:+2657219 MS.gene21685:CDS
ATGAGTCAGTTAGAAGAAAC+AGG 0.431891 5.2:+2659989 MS.gene21685:CDS
GAGACGAGAGTTACGGTTAG+AGG 0.454033 5.2:-2657356 None:intergenic
GCGGATTTGAGTTTGGGATG+TGG 0.456064 5.2:-2657277 None:intergenic
CAAAGGTCGCGGTTTCCGTC+AGG 0.458944 5.2:+2657324 MS.gene21685:CDS
TTGAATCTTGATCGCCGTAC+TGG 0.470728 5.2:+2657628 MS.gene21685:CDS
AGAGTTACGGTTAGAGGAAT+CGG 0.477329 5.2:-2657350 None:intergenic
TCCATCAAGTCATCTTCCTC+TGG 0.480725 5.2:-2657220 None:intergenic
GTTGATTGGGCTTTCAGCAG+TGG 0.486700 5.2:+2659958 MS.gene21685:CDS
CTCTATTCAGCGACGATGCA+AGG 0.487696 5.2:+2657163 None:intergenic
AATGCAATAGAGAATTTGAA+TGG 0.500993 5.2:+2659907 MS.gene21685:CDS
TGGGGATACGAACATCTTTG+AGG 0.513262 5.2:-2657424 None:intergenic
GGGATGTGGAGGAGAAGCTT+CGG 0.519462 5.2:-2657263 None:intergenic
AGCAGTGAGAGTATCGAAAC+CGG 0.528470 5.2:-2657386 None:intergenic
TTCGATACTCTCACTGCTGA+GGG 0.535196 5.2:+2657391 MS.gene21685:CDS
ATGCAAGGAAGCGCCGCCGA+CGG 0.548735 5.2:+2657178 MS.gene21685:CDS
ATGAGCGCTCTGAGGAAGCT+CGG 0.562915 5.2:+2659884 MS.gene21685:CDS
AATCAGCGAGACGAGAGTTA+CGG 0.563114 5.2:-2657363 None:intergenic
TACGAACATCTTTGAGGACC+AGG 0.563747 5.2:-2657418 None:intergenic
GATGACTTGATGGACGAAGA+AGG 0.572561 5.2:+2657229 MS.gene21685:CDS
GATTTGAGTTTGGGATGTGG+AGG 0.573507 5.2:-2657274 None:intergenic
AGCTTTGGATTTCGAGCCAG+AGG 0.579650 5.2:+2657204 MS.gene21685:CDS
ATCCAAAGCTTCTCCGTCGG+CGG 0.583085 5.2:-2657191 None:intergenic
TTTCGATACTCTCACTGCTG+AGG 0.590321 5.2:+2657390 MS.gene21685:CDS
TTTGGGAACGGAAGTGATGG+CGG 0.614347 5.2:-2657296 None:intergenic
GATACTCTCACTGCTGAGGG+TGG 0.647746 5.2:+2657394 MS.gene21685:CDS
GCACCTTAACAAAACCAGTA+CGG 0.658816 5.2:-2657642 None:intergenic
TTTGGTGACTGGTGTACATG+AGG 0.661358 5.2:+2657543 MS.gene21685:CDS
TAGAGGAATCGGAATCCTGA+CGG 0.677725 5.2:-2657339 None:intergenic
GATTGAATATGAGCGCTCTG+AGG 0.688874 5.2:+2659876 MS.gene21685:CDS
GAAATCCAAAGCTTCTCCGT+CGG 0.701772 5.2:-2657194 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCTTAATAAAATAAGATA+AGG - chr5.2:2659597-2659616 None:intergenic 10.0%
!!! TATCTTATTTTATTAAGAAA+AGG + chr5.2:2659597-2659616 MS.gene21685:intron 10.0%
!! AAATTGACAAAAATTGAAAT+TGG - chr5.2:2657447-2657466 None:intergenic 15.0%
!! AATTGACAAAAATTGAAATT+GGG - chr5.2:2657446-2657465 None:intergenic 15.0%
!! TTGAAAAAAAAAAAAAAGGA+AGG + chr5.2:2658372-2658391 MS.gene21685:intron 15.0%
!!! TACTTTTCATTCTTAAATTA+TGG + chr5.2:2657883-2657902 MS.gene21685:intron 15.0%
!! AAGAAGAAAAAAAAAAAGGA+AGG - chr5.2:2658267-2658286 None:intergenic 20.0%
!! ATTGACAAAAATTGAAATTG+GGG - chr5.2:2657445-2657464 None:intergenic 20.0%
!! TTGAAAAAAAAAATACTGCA+GGG - chr5.2:2659394-2659413 None:intergenic 20.0%
!! TTGAGAAATATATGGAAAAA+TGG - chr5.2:2658576-2658595 None:intergenic 20.0%
!! TTGGAAGAAGAAAAAAAAAA+AGG - chr5.2:2658271-2658290 None:intergenic 20.0%
!! TTTATAGACAGTGTATATTA+CGG - chr5.2:2658766-2658785 None:intergenic 20.0%
!! TTTGAAAAAAAAAATACTGC+AGG - chr5.2:2659395-2659414 None:intergenic 20.0%
!!! AATGCAGTTTTGATTTTTAA+AGG + chr5.2:2659441-2659460 MS.gene21685:intron 20.0%
!!! GGCTTTGAAAAAAAAAAAAA+AGG + chr5.2:2658368-2658387 MS.gene21685:intron 20.0%
!!! TACTTGTAATTTTGTAAACA+GGG + chr5.2:2659640-2659659 MS.gene21685:intron 20.0%
!!! TGATAAGCATTTTGATTTTA+GGG + chr5.2:2659844-2659863 MS.gene21685:intron 20.0%
! AAAACAAAACCCTCATAATT+TGG - chr5.2:2658181-2658200 None:intergenic 25.0%
! AAACAAAACCCTCATAATTT+GGG - chr5.2:2658180-2658199 None:intergenic 25.0%
! AAGTTGCTAAATAAAGTTAC+AGG - chr5.2:2659772-2659791 None:intergenic 25.0%
! AATGCAATAGAGAATTTGAA+TGG + chr5.2:2659907-2659926 MS.gene21685:CDS 25.0%
! AATTCAAGTCATGAATCAAA+TGG + chr5.2:2658860-2658879 MS.gene21685:intron 25.0%
! AGTGTATTAATCTATGTTGT+TGG - chr5.2:2658054-2658073 None:intergenic 25.0%
! CAAATCTTCACATGATAAAA+TGG - chr5.2:2659814-2659833 None:intergenic 25.0%
! CAGAAGGTTAACAATAAAAA+AGG - chr5.2:2658651-2658670 None:intergenic 25.0%
! CTAAATAAAGTTACAGGAAA+AGG - chr5.2:2659766-2659785 None:intergenic 25.0%
! GTGTATCATTGTATCTATAT+GGG - chr5.2:2659006-2659025 None:intergenic 25.0%
! GTGTATTAATCTATGTTGTT+GGG - chr5.2:2658053-2658072 None:intergenic 25.0%
! TCATGACTTGAATTATTTGA+AGG - chr5.2:2658854-2658873 None:intergenic 25.0%
! TGTGTATCATTGTATCTATA+TGG - chr5.2:2659007-2659026 None:intergenic 25.0%
! TTACCATTAATCAATCACTT+AGG - chr5.2:2658613-2658632 None:intergenic 25.0%
!! ACAAACCATTTATGTTGATT+GGG + chr5.2:2659945-2659964 MS.gene21685:CDS 25.0%
!! CATTTTATCATGTGAAGATT+TGG + chr5.2:2659812-2659831 MS.gene21685:intron 25.0%
!! CTACTTGTAATTTTGTAAAC+AGG + chr5.2:2659639-2659658 MS.gene21685:intron 25.0%
!! TGAATCAGAAGATCTATTTT+CGG + chr5.2:2658721-2658740 MS.gene21685:intron 25.0%
!! TTTGAAGTAGGAAATGTATA+TGG + chr5.2:2657799-2657818 MS.gene21685:intron 25.0%
!!! AATTGCGTGATTTTGATTTT+AGG + chr5.2:2657467-2657486 MS.gene21685:intron 25.0%
!!! ATTGCGTGATTTTGATTTTA+GGG + chr5.2:2657468-2657487 MS.gene21685:intron 25.0%
!!! CTGATAAGCATTTTGATTTT+AGG + chr5.2:2659843-2659862 MS.gene21685:intron 25.0%
!!! CTTTTTTATTGTTAACCTTC+TGG + chr5.2:2658649-2658668 MS.gene21685:intron 25.0%
!!! GTATAGATTGACTAGTTTTA+TGG + chr5.2:2657741-2657760 MS.gene21685:intron 25.0%
!!! TATTGAAGGATGGATTATTT+TGG + chr5.2:2657525-2657544 MS.gene21685:CDS 25.0%
!!! TTGATTTTTAAAGGATGTAG+TGG + chr5.2:2659450-2659469 MS.gene21685:intron 25.0%
AACAAAACCCTCATAATTTG+GGG - chr5.2:2658179-2658198 None:intergenic 30.0%
AAGCCTAAGTGATTGATTAA+TGG + chr5.2:2658607-2658626 MS.gene21685:intron 30.0%
AAGTAGGAAATGTATATGGA+CGG + chr5.2:2657803-2657822 MS.gene21685:intron 30.0%
ATTAATCTATGTTGTTGGGA+GGG - chr5.2:2658049-2658068 None:intergenic 30.0%
CACAAACCATTTATGTTGAT+TGG + chr5.2:2659944-2659963 MS.gene21685:CDS 30.0%
CGATTCTCATTTCATTCTAA+TGG - chr5.2:2658950-2658969 None:intergenic 30.0%
TATGAACATAGAAACAGAGA+TGG - chr5.2:2659693-2659712 None:intergenic 30.0%
TATTAATCTATGTTGTTGGG+AGG - chr5.2:2658050-2658069 None:intergenic 30.0%
TGAGATGATAACTTCAGTTA+AGG - chr5.2:2659198-2659217 None:intergenic 30.0%
TGGATGAAATAGAAGACATT+GGG + chr5.2:2658401-2658420 MS.gene21685:intron 30.0%
TTGGGAGTTTGAGAAATATA+TGG - chr5.2:2658584-2658603 None:intergenic 30.0%
! AAAATTTGATAACCATGCTC+CGG - chr5.2:2658702-2658721 None:intergenic 30.0%
! AATCTAAGGGTATGATTGTA+GGG + chr5.2:2657834-2657853 MS.gene21685:intron 30.0%
! TAATCTAAGGGTATGATTGT+AGG + chr5.2:2657833-2657852 MS.gene21685:intron 30.0%
! TTTTTGTAGCTATTGAAGGA+TGG + chr5.2:2657515-2657534 MS.gene21685:intron 30.0%
!! GAGAATCGTTTTAGTGAATT+GGG + chr5.2:2658962-2658981 MS.gene21685:intron 30.0%
!! TGAGAATCGTTTTAGTGAAT+TGG + chr5.2:2658961-2658980 MS.gene21685:intron 30.0%
!!! GATAGAAAGTGTTTTGAAGT+AGG + chr5.2:2657787-2657806 MS.gene21685:intron 30.0%
AAAAAAAAGGAAGGAGCAAG+TGG + chr5.2:2658381-2658400 MS.gene21685:intron 35.0%
AAAAAAAAGGAAGGAGCAAG+TGG - chr5.2:2658400-2658381 None:intergenic 35.0%
AAACTCCACATCATGGTTAT+CGG - chr5.2:2659099-2659118 None:intergenic 35.0%
AACTAGTCAATCTATACGAG+TGG - chr5.2:2657738-2657757 None:intergenic 35.0%
AATACAAAATCCCCCTCATT+TGG + chr5.2:2658457-2658476 MS.gene21685:intron 35.0%
ACAAAACCCTCATAATTTGG+GGG - chr5.2:2658178-2658197 None:intergenic 35.0%
ACAATGATACACACTAACTG+AGG + chr5.2:2659014-2659033 MS.gene21685:intron 35.0%
ACACTAACTGAGGTTATTGA+TGG + chr5.2:2659024-2659043 MS.gene21685:intron 35.0%
ACTAGTCAATCTATACGAGT+GGG - chr5.2:2657737-2657756 None:intergenic 35.0%
ACTCAAAATTGTATTCGAGG+AGG - chr5.2:2658153-2658172 None:intergenic 35.0%
AGTAGGAAATGTATATGGAC+GGG + chr5.2:2657804-2657823 MS.gene21685:intron 35.0%
AGTGAATTGGGATTCAAGAT+GGG + chr5.2:2658974-2658993 MS.gene21685:intron 35.0%
ATACAAAATCCCCCTCATTT+GGG + chr5.2:2658458-2658477 MS.gene21685:intron 35.0%
ATGAAATAGAAGACATTGGG+AGG + chr5.2:2658404-2658423 MS.gene21685:intron 35.0%
ATGAGTCAGTTAGAAGAAAC+AGG + chr5.2:2659989-2660008 MS.gene21685:CDS 35.0%
CACTAACTGAGGTTATTGAT+GGG + chr5.2:2659025-2659044 MS.gene21685:intron 35.0%
CTCAAAATTGTATTCGAGGA+GGG - chr5.2:2658152-2658171 None:intergenic 35.0%
GAAAGCCCAATCAACATAAA+TGG - chr5.2:2659953-2659972 None:intergenic 35.0%
GAGATGGCAGAAAATTTACT+TGG - chr5.2:2659677-2659696 None:intergenic 35.0%
GGAAAGTTAAGTCCAACTAA+CGG - chr5.2:2659231-2659250 None:intergenic 35.0%
GGAACTCAAAATTGTATTCG+AGG - chr5.2:2658156-2658175 None:intergenic 35.0%
GTGGATGAAATAGAAGACAT+TGG + chr5.2:2658400-2658419 MS.gene21685:intron 35.0%
TAGTGAATTGGGATTCAAGA+TGG + chr5.2:2658973-2658992 MS.gene21685:intron 35.0%
TGAAATAGAAGACATTGGGA+GGG + chr5.2:2658405-2658424 MS.gene21685:intron 35.0%
TTAATCTATGTTGTTGGGAG+GGG - chr5.2:2658048-2658067 None:intergenic 35.0%
! AATATCAAGGTACCTTTCCT+CGG - chr5.2:2658679-2658698 None:intergenic 35.0%
! CAGAATGCTTTTGGTGAATA+TGG + chr5.2:2657586-2657605 MS.gene21685:CDS 35.0%
! CATATCTTTTCACCGTTAGT+TGG + chr5.2:2659216-2659235 MS.gene21685:intron 35.0%
! CTAAGGCTTTGTCTTTGTTT+GGG - chr5.2:2658317-2658336 None:intergenic 35.0%
! CTTCTAACTGACTCATTGAT+AGG - chr5.2:2659985-2660004 None:intergenic 35.0%
! GAATTATAGCCAGCTTTTGT+TGG - chr5.2:2658908-2658927 None:intergenic 35.0%
! TCTTTGTTTGGGAGTTTAGA+GGG - chr5.2:2658306-2658325 None:intergenic 35.0%
!! AAATGTATATGGACGGGTTA+TGG + chr5.2:2657810-2657829 MS.gene21685:intron 35.0%
!! CAATCACTTAGGCTTTGTTT+GGG - chr5.2:2658602-2658621 None:intergenic 35.0%
!! GATGATTTGCAGAATGCTTT+TGG + chr5.2:2657577-2657596 MS.gene21685:CDS 35.0%
!! TCAATCACTTAGGCTTTGTT+TGG - chr5.2:2658603-2658622 None:intergenic 35.0%
!!! ACCTTTGGTTTTTTTGGGAA+CGG - chr5.2:2657311-2657330 None:intergenic 35.0%
!!! GCTGTTTTTGTAGCTATTGA+AGG + chr5.2:2657511-2657530 MS.gene21685:intron 35.0%
!!! TATGATTGTAGGGTTTTGGA+GGG + chr5.2:2657844-2657863 MS.gene21685:intron 35.0%
AAACTCCCAAACTAAGCCTA+AGG - chr5.2:2658334-2658353 None:intergenic 40.0%
ACAAAGCCTTAGGCTTAGTT+TGG + chr5.2:2658325-2658344 MS.gene21685:intron 40.0%
AGAGTTACGGTTAGAGGAAT+CGG - chr5.2:2657353-2657372 None:intergenic 40.0%
AGATACCGATAACCATGATG+TGG + chr5.2:2659091-2659110 MS.gene21685:intron 40.0%
AGGAAATAGAGGAGTTTGAG+AGG - chr5.2:2658235-2658254 None:intergenic 40.0%
ATTGAGAGGTAGGTAAAGTG+AGG + chr5.2:2659120-2659139 MS.gene21685:intron 40.0%
CAAAACCCTCATAATTTGGG+GGG - chr5.2:2658177-2658196 None:intergenic 40.0%
CAAAGCCTTAGGCTTAGTTT+GGG + chr5.2:2658326-2658345 MS.gene21685:intron 40.0%
CAATGTTGACCAACAAAAGC+TGG + chr5.2:2658896-2658915 MS.gene21685:intron 40.0%
CAGTTAAGGTTGCATCAATC+AGG - chr5.2:2659184-2659203 None:intergenic 40.0%
CAGTTTCAGAAACTGCAGAT+TGG + chr5.2:2659736-2659755 MS.gene21685:intron 40.0%
CCAAACAAAGACAAAGCCTT+AGG + chr5.2:2658315-2658334 MS.gene21685:intron 40.0%
CCTAACAAGTAACTGTGTGT+AGG + chr5.2:2659491-2659510 MS.gene21685:intron 40.0%
CCTACACACAGTTACTTGTT+AGG - chr5.2:2659494-2659513 None:intergenic 40.0%
CTCAATCAAACTCCACATCA+TGG - chr5.2:2659106-2659125 None:intergenic 40.0%
GAAATAGAAGACATTGGGAG+GGG + chr5.2:2658406-2658425 MS.gene21685:intron 40.0%
GAAATAGAGGAGTTTGAGAG+GGG - chr5.2:2658233-2658252 None:intergenic 40.0%
GCACCTTAACAAAACCAGTA+CGG - chr5.2:2657645-2657664 None:intergenic 40.0%
GGAAATAGAGGAGTTTGAGA+GGG - chr5.2:2658234-2658253 None:intergenic 40.0%
TACAAAATCCCCCTCATTTG+GGG + chr5.2:2658459-2658478 MS.gene21685:intron 40.0%
TCATGAATCAAATGGCAAGC+TGG + chr5.2:2658868-2658887 MS.gene21685:intron 40.0%
TCCATTTCATGTCTCAGTCA+TGG + chr5.2:2659248-2659267 MS.gene21685:intron 40.0%
TCCGTTCCCAAAAAAACCAA+AGG + chr5.2:2657307-2657326 MS.gene21685:CDS 40.0%
TGATGTGGAGTTTGATTGAG+AGG + chr5.2:2659106-2659125 MS.gene21685:intron 40.0%
! AGGGTATGATTGTAGGGTTT+TGG + chr5.2:2657840-2657859 MS.gene21685:intron 40.0%
! ATATCAAGGTACCTTTCCTC+GGG - chr5.2:2658678-2658697 None:intergenic 40.0%
! CACACAGTTACTTGTTAGGA+AGG - chr5.2:2659490-2659509 None:intergenic 40.0%
! CCTAAGGCTTTGTCTTTGTT+TGG - chr5.2:2658318-2658337 None:intergenic 40.0%
! GCTTAGTTTGGGAGTTTAGA+GGG + chr5.2:2658337-2658356 MS.gene21685:intron 40.0%
! GTCTTTGTTTGGGAGTTTAG+AGG - chr5.2:2658307-2658326 None:intergenic 40.0%
! TTGACCTCAAGCTGTTACTT+TGG + chr5.2:2659304-2659323 MS.gene21685:intron 40.0%
!! GACGGGTTATGGTAATCTAA+GGG + chr5.2:2657821-2657840 MS.gene21685:intron 40.0%
!! GGAAAGGTACCTTGATATTC+CGG + chr5.2:2658680-2658699 MS.gene21685:intron 40.0%
!! GTATGATTGTAGGGTTTTGG+AGG + chr5.2:2657843-2657862 MS.gene21685:intron 40.0%
!! TGACCTCAAGCTGTTACTTT+GGG + chr5.2:2659305-2659324 MS.gene21685:intron 40.0%
!!! ATGATTGTAGGGTTTTGGAG+GGG + chr5.2:2657845-2657864 MS.gene21685:intron 40.0%
!!! GGATGGATTATTTTGGTGAC+TGG + chr5.2:2657532-2657551 MS.gene21685:CDS 40.0%
!!! TATTCGAGGAGGGTTTTGAA+GGG - chr5.2:2658142-2658161 None:intergenic 40.0%
!!! TTTTGAGTTCCCCCAAATGA+GGG - chr5.2:2658472-2658491 None:intergenic 40.0%
!!! TTTTTGAGTTCCCCCAAATG+AGG - chr5.2:2658473-2658492 None:intergenic 40.0%
!!! TTTTTTGGGAACGGAAGTGA+TGG - chr5.2:2657302-2657321 None:intergenic 40.0%
AAAAAGGAAGGAGCAAGTGG+AGG - chr5.2:2658403-2658384 None:intergenic 45.0%
AACCATGCTCCGGAATATCA+AGG - chr5.2:2658692-2658711 None:intergenic 45.0%
AACCCCAAAGTAACAGCTTG+AGG - chr5.2:2659311-2659330 None:intergenic 45.0%
AATCAGCGAGACGAGAGTTA+CGG - chr5.2:2657366-2657385 None:intergenic 45.0%
ACAAAATCCCCCTCATTTGG+GGG + chr5.2:2658460-2658479 MS.gene21685:intron 45.0%
CCCAAAAAAACCAAAGGTCG+CGG + chr5.2:2657313-2657332 MS.gene21685:CDS 45.0%
GAAATCCAAAGCTTCTCCGT+CGG - chr5.2:2657197-2657216 None:intergenic 45.0%
GAGTTCCCCCCAAATTATGA+GGG + chr5.2:2658169-2658188 MS.gene21685:intron 45.0%
GATTGAATATGAGCGCTCTG+AGG + chr5.2:2659876-2659895 MS.gene21685:CDS 45.0%
GCCATGACTGAGACATGAAA+TGG - chr5.2:2659252-2659271 None:intergenic 45.0%
GGAGTTTAGAGGGAAGTGAA+GGG + chr5.2:2658347-2658366 MS.gene21685:intron 45.0%
GTGGAGTTTGATTGAGAGGT+AGG + chr5.2:2659110-2659129 MS.gene21685:intron 45.0%
TACCTTGATATTCCGGAGCA+TGG + chr5.2:2658687-2658706 MS.gene21685:intron 45.0%
TAGAGGAATCGGAATCCTGA+CGG - chr5.2:2657342-2657361 None:intergenic 45.0%
TCCATCAAGTCATCTTCCTC+TGG - chr5.2:2657223-2657242 None:intergenic 45.0%
TCCCATGCTGGAACAATCTT+CGG - chr5.2:2659146-2659165 None:intergenic 45.0%
TGAGATCAAAGCAGCGAAAG+TGG - chr5.2:2659574-2659593 None:intergenic 45.0%
TGAGTTCCCCCCAAATTATG+AGG + chr5.2:2658168-2658187 MS.gene21685:intron 45.0%
TGGGGATACGAACATCTTTG+AGG - chr5.2:2657427-2657446 None:intergenic 45.0%
TTCATGTCTCAGTCATGGCA+TGG + chr5.2:2659253-2659272 MS.gene21685:intron 45.0%
TTCGATACTCTCACTGCTGA+GGG + chr5.2:2657391-2657410 MS.gene21685:CDS 45.0%
TTGAATCTTGATCGCCGTAC+TGG + chr5.2:2657628-2657647 MS.gene21685:CDS 45.0%
TTTCGATACTCTCACTGCTG+AGG + chr5.2:2657390-2657409 MS.gene21685:CDS 45.0%
TTTGGGAGTTTAGAGGGCAA+GGG - chr5.2:2658300-2658319 None:intergenic 45.0%
! GATGACTTGATGGACGAAGA+AGG + chr5.2:2657229-2657248 MS.gene21685:CDS 45.0%
! GATTTGAGTTTGGGATGTGG+AGG - chr5.2:2657277-2657296 None:intergenic 45.0%
! GGCTTAGTTTGGGAGTTTAG+AGG + chr5.2:2658336-2658355 MS.gene21685:intron 45.0%
! TACGAACATCTTTGAGGACC+AGG - chr5.2:2657421-2657440 None:intergenic 45.0%
! TATGTTGTTGGGAGGGGTTT+TGG - chr5.2:2658042-2658061 None:intergenic 45.0%
! TTTGAGTTCCCCCAAATGAG+GGG - chr5.2:2658471-2658490 None:intergenic 45.0%
! TTTGGTGACTGGTGTACATG+AGG + chr5.2:2657543-2657562 MS.gene21685:CDS 45.0%
!! AGCAGTGAGAGTATCGAAAC+CGG - chr5.2:2657389-2657408 None:intergenic 45.0%
!! AGTGATGGCGGATTTGAGTT+TGG - chr5.2:2657287-2657306 None:intergenic 45.0%
!! CTGTTACTTTGGGGTTCAGA+TGG + chr5.2:2659315-2659334 MS.gene21685:intron 45.0%
!! GACCTCAAGCTGTTACTTTG+GGG + chr5.2:2659306-2659325 MS.gene21685:intron 45.0%
!! GGACGGGTTATGGTAATCTA+AGG + chr5.2:2657820-2657839 MS.gene21685:intron 45.0%
!! GTATTCGAGGAGGGTTTTGA+AGG - chr5.2:2658143-2658162 None:intergenic 45.0%
!! GTGATGGCGGATTTGAGTTT+GGG - chr5.2:2657286-2657305 None:intergenic 45.0%
!!! ACCGCGACCTTTGGTTTTTT+TGG - chr5.2:2657317-2657336 None:intergenic 45.0%
!!! AGGAGGGTTTTGAAGGGATT+TGG - chr5.2:2658136-2658155 None:intergenic 45.0%
!!! CCGCGACCTTTGGTTTTTTT+GGG - chr5.2:2657316-2657335 None:intergenic 45.0%
!!! GGGTTTTGAAGGGATTTGGA+AGG - chr5.2:2658132-2658151 None:intergenic 45.0%
!!! TCGCCGTACTGGTTTTGTTA+AGG + chr5.2:2657639-2657658 MS.gene21685:CDS 45.0%
!!! TGTATATAAATTTTATAAAA+TGG + chr5.2:2659356-2659375 MS.gene21685:intron 5.0%
ATAGAAGACATTGGGAGGGG+AGG + chr5.2:2658409-2658428 MS.gene21685:intron 50.0%
ATAGAGGAGTTTGAGAGGGG+AGG - chr5.2:2658230-2658249 None:intergenic 50.0%
GAGACGAGAGTTACGGTTAG+AGG - chr5.2:2657359-2657378 None:intergenic 50.0%
GCCAGAGGAAGATGACTTGA+TGG + chr5.2:2657219-2657238 MS.gene21685:CDS 50.0%
GCCGAAGATTGTTCCAGCAT+GGG + chr5.2:2659142-2659161 MS.gene21685:intron 50.0%
GGAGCAAGTGGAGGAAATAG+AGG - chr5.2:2658246-2658265 None:intergenic 50.0%
GGGAGTTTAGAGGGAAGTGA+AGG + chr5.2:2658346-2658365 MS.gene21685:intron 50.0%
GTTGATTGGGCTTTCAGCAG+TGG + chr5.2:2659958-2659977 MS.gene21685:CDS 50.0%
GTTTGGGAGTTTAGAGGGCA+AGG - chr5.2:2658301-2658320 None:intergenic 50.0%
TAGAAGACATTGGGAGGGGA+GGG + chr5.2:2658410-2658429 MS.gene21685:intron 50.0%
TAGAGGAGTTTGAGAGGGGA+GGG - chr5.2:2658229-2658248 None:intergenic 50.0%
TCCAGCATGGGATCGAACTT+GGG + chr5.2:2659154-2659173 MS.gene21685:intron 50.0%
TTCCAGCATGGGATCGAACT+TGG + chr5.2:2659153-2659172 MS.gene21685:intron 50.0%
TTGAGTTCCCCCAAATGAGG+GGG - chr5.2:2658470-2658489 None:intergenic 50.0%
TTTGGGAACGGAAGTGATGG+CGG - chr5.2:2657299-2657318 None:intergenic 50.0%
! GCGGATTTGAGTTTGGGATG+TGG - chr5.2:2657280-2657299 None:intergenic 50.0%
!! AACTCTCGTCTCGCTGATTC+CGG + chr5.2:2657367-2657386 MS.gene21685:CDS 50.0%
!! AGCTTTGGATTTCGAGCCAG+AGG + chr5.2:2657204-2657223 MS.gene21685:CDS 50.0%
!! GTTAACCTTCTGGTTTCCCG+AGG + chr5.2:2658659-2658678 MS.gene21685:intron 50.0%
!!! ATTGTAGGGTTTTGGAGGGG+AGG + chr5.2:2657848-2657867 MS.gene21685:intron 50.0%
!!! TTGTAGGGTTTTGGAGGGGA+GGG + chr5.2:2657849-2657868 MS.gene21685:intron 50.0%
ACCCAAGTTCGATCCCATGC+TGG - chr5.2:2659158-2659177 None:intergenic 55.0%
AGTTTGAGAGGGGAGGGCTT+TGG - chr5.2:2658223-2658242 None:intergenic 55.0%
ATCCAAAGCTTCTCCGTCGG+CGG - chr5.2:2657194-2657213 None:intergenic 55.0%
CCTTTCCTCGGGAAACCAGA+AGG - chr5.2:2658667-2658686 None:intergenic 55.0%
GATACTCTCACTGCTGAGGG+TGG + chr5.2:2657394-2657413 MS.gene21685:CDS 55.0%
GGAGTTTAGAGGGCAAGGGA+GGG - chr5.2:2658296-2658315 None:intergenic 55.0%
GGCCGAAGATTGTTCCAGCA+TGG + chr5.2:2659141-2659160 MS.gene21685:intron 55.0%
GGGATGTGGAGGAGAAGCTT+CGG - chr5.2:2657266-2657285 None:intergenic 55.0%
GTTTGAGAGGGGAGGGCTTT+GGG - chr5.2:2658222-2658241 None:intergenic 55.0%
TTTGAGAGGGGAGGGCTTTG+GGG - chr5.2:2658221-2658240 None:intergenic 55.0%
! ATACTGCAGGGAGTGCAGTG+AGG - chr5.2:2659382-2659401 None:intergenic 55.0%
! ATTGGGAGGGGAGGGCTTTT+GGG + chr5.2:2658418-2658437 MS.gene21685:intron 55.0%
! TAGAGGGCAAGGGAGGGTTT+TGG - chr5.2:2658290-2658309 None:intergenic 55.0%
!! ATGAGCGCTCTGAGGAAGCT+CGG + chr5.2:2659884-2659903 MS.gene21685:CDS 55.0%
!! CCTTCTGGTTTCCCGAGGAA+AGG + chr5.2:2658664-2658683 MS.gene21685:intron 55.0%
!! GTTGTTGGGAGGGGTTTTGG+AGG - chr5.2:2658039-2658058 None:intergenic 55.0%
!!! TGTAGGGTTTTGGAGGGGAG+GGG + chr5.2:2657850-2657869 MS.gene21685:intron 55.0%
CAAAGGTCGCGGTTTCCGTC+AGG + chr5.2:2657324-2657343 MS.gene21685:CDS 60.0%
CTGACGGAAACCGCGACCTT+TGG - chr5.2:2657326-2657345 None:intergenic 60.0%
GGGAGTTTAGAGGGCAAGGG+AGG - chr5.2:2658297-2658316 None:intergenic 60.0%
! CATTGGGAGGGGAGGGCTTT+TGG + chr5.2:2658417-2658436 MS.gene21685:intron 60.0%
! TTGAGAGGGGAGGGCTTTGG+GGG - chr5.2:2658220-2658239 None:intergenic 60.0%
! TTGGGAGGGGAGGGCTTTTG+GGG + chr5.2:2658419-2658438 MS.gene21685:intron 60.0%
!!! GTAGGGTTTTGGAGGGGAGG+GGG + chr5.2:2657851-2657870 MS.gene21685:intron 60.0%
CTCACTGCTGAGGGTGGTCC+TGG + chr5.2:2657400-2657419 MS.gene21685:CDS 65.0%
!! ATGCAAGGAAGCGCCGCCGA+CGG + chr5.2:2657178-2657197 MS.gene21685:CDS 65.0%
!! TGAGAGGGGAGGGCTTTGGG+GGG - chr5.2:2658219-2658238 None:intergenic 65.0%
!! TTTTGGAGGGGAGGGGGTGG+AGG + chr5.2:2657857-2657876 MS.gene21685:intron 65.0%
CGCCGCCGACGGAGAAGCTT+TGG + chr5.2:2657189-2657208 MS.gene21685:CDS 70.0%
!!! GGGTTTTGGAGGGGAGGGGG+TGG + chr5.2:2657854-2657873 MS.gene21685:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 2657178 2660014 2657178 ID=MS.gene21685
chr5.2 mRNA 2657178 2660014 2657178 ID=MS.gene21685.t1;Parent=MS.gene21685
chr5.2 exon 2657178 2657433 2657178 ID=MS.gene21685.t1.exon1;Parent=MS.gene21685.t1
chr5.2 CDS 2657178 2657433 2657178 ID=cds.MS.gene21685.t1;Parent=MS.gene21685.t1
chr5.2 exon 2657524 2657660 2657524 ID=MS.gene21685.t1.exon2;Parent=MS.gene21685.t1
chr5.2 CDS 2657524 2657660 2657524 ID=cds.MS.gene21685.t1;Parent=MS.gene21685.t1
chr5.2 exon 2659865 2660014 2659865 ID=MS.gene21685.t1.exon3;Parent=MS.gene21685.t1
chr5.2 CDS 2659865 2660014 2659865 ID=cds.MS.gene21685.t1;Parent=MS.gene21685.t1
Gene Sequence

>MS.gene21685

ATGCAAGGAAGCGCCGCCGACGGAGAAGCTTTGGATTTCGAGCCAGAGGAAGATGACTTGATGGACGAAGAAGGTGCTGTTGACGCCGAAGCTTCTCCTCCACATCCCAAACTCAAATCCGCCATCACTTCCGTTCCCAAAAAAACCAAAGGTCGCGGTTTCCGTCAGGATTCCGATTCCTCTAACCGTAACTCTCGTCTCGCTGATTCCGGTTTCGATACTCTCACTGCTGAGGGTGGTCCTGGTCCTCAAAGATGTTCGTATCCCCAATTTCAATTTTTGTCAATTTAATTGCGTGATTTTGATTTTAGGGTTTTTATGCTTAATAATGTTGCTGTTTTTGTAGCTATTGAAGGATGGATTATTTTGGTGACTGGTGTACATGAGGAAGCTCAAGATGATGATTTGCAGAATGCTTTTGGTGAATATGGTGATATTAAGAATCTTCACTTGAATCTTGATCGCCGTACTGGTTTTGTTAAGGTGCGTTGTTGATGATTTTTTGCTATTCACTTCACTGTATTTTTTATGATACACTTGTTCTTGTGCATTGTTACCCACTCGTATAGATTGACTAGTTTTATGGATAATTTGTTATCATAAATTGATGATAGAAAGTGTTTTGAAGTAGGAAATGTATATGGACGGGTTATGGTAATCTAAGGGTATGATTGTAGGGTTTTGGAGGGGAGGGGGTGGAGGCTTTACTTTTCATTCTTAAATTATGGTTACTATAAAATGCTTTTTAAAATCAATTAAGTTTGATTGAATCGTTTTTTTGATTGAATCGTTTATCATGTTCTTCTTTGATTTTTTTTTTTTTTAAATAACAAATATATTTTAACATATTGACTTAGACCTCCAAAACCCCTCCCAACAACATAGATTAATACACTTGTAATTTTAAGAATATTACAACAACATAAATTGAATTTGAATAATTCGTCTAAACCTTCCAAATCCCTTCAAAACCCTCCTCGAATACAATTTTGAGTTCCCCCCAAATTATGAGGGTTTTGTTTTATGAAGAAAAATTAACCCCCCAAAGCCCTCCCCTCTCAAACTCCTCTATTTCCTCCACTTGCTCCTTCCTTTTTTTTTTTCTTCTTCCAAAACCCTCCCTTGCCCTCTAAACTCCCAAACAAAGACAAAGCCTTAGGCTTAGTTTGGGAGTTTAGAGGGAAGTGAAGGGCTTTGAAAAAAAAAAAAAAGGAAGGAGCAAGTGGATGAAATAGAAGACATTGGGAGGGGAGGGCTTTTGGGGTTCATGTTTCTTCATAATACAAAATCCCCCTCATTTGGGGGAACTCAAAAATTGTATTAATTGTTGTTCTAATATTTCTTAAAATTAAAAATATATTTATCATATTCGTTTATCATTCTCGTGAAAGATCTCCATTTTTCCATATATTTCTCAAACTCCCAAACAAAGCCTAAGTGATTGATTAATGGTAATATTTATGTGATGCTCCTTTTTTATTGTTAACCTTCTGGTTTCCCGAGGAAAGGTACCTTGATATTCCGGAGCATGGTTATCAAATTTTGAATCAGAAGATCTATTTTCGGAATTGAATCTTTTTATGAACCGTAATATACACTGTCTATAAAAATATGACATTTTTAAGTAAGACAAGTGACATAGCAAAATTATAAGCTAATATATCATATATGAACCTTCAAATAATTCAAGTCATGAATCAAATGGCAAGCTGGCTATACAATGTTGACCAACAAAAGCTGGCTATAATTCAAATAATTTTGATATAGAACCATTAGAATGAAATGAGAATCGTTTTAGTGAATTGGGATTCAAGATGGGATTCATCCCATATAGATACAATGATACACACTAACTGAGGTTATTGATGGGTTCTGTTTTGTATCGAATTGAGATACAACTCACATTTATTTTAAGATACCGATAACCATGATGTGGAGTTTGATTGAGAGGTAGGTAAAGTGAGGCCGAAGATTGTTCCAGCATGGGATCGAACTTGGGTACTCCTGATTGATGCAACCTTAACTGAAGTTATCATCTCATATCTTTTCACCGTTAGTTGGACTTAACTTTCCATTTCATGTCTCAGTCATGGCATGGATTCTGCTTTGCTGCACACATTTTGTTATTGACCTCAAGCTGTTACTTTGGGGTTCAGATGGTTGTTGATTTGTTTCTCATGTATATAAATTTTATAAAATGGCCTCACTGCACTCCCTGCAGTATTTTTTTTTTCAAATTGATCTTGCTCTATCAGTATAAATCAATGCAGTTTTGATTTTTAAAGGATGTAGTGGATGAAACATATAATCCTTCCTAACAAGTAACTGTGTGTAGGTTATTGTCGTTTCTTTTGTGTTTTATATTTTTATGATGATGCTTTTACTCTTGTTTGCCACTTTCGCTGCTTTGATCTCACCTTATCTTATTTTATTAAGAAAAGGTAAGACTCTTTTTTTTAGACTACTTGTAATTTTGTAAACAGGGTTGATACTTGTCCAAGTAAATTTTCTGCCATCTCTGTTTCTATGTTCATACATCATTGATTGATGATTAATATCAGTTTCAGAAACTGCAGATTGGTTCTCCTTTTCCTGTAACTTTATTTAGCAACTTCTGCTCATTTCTGCATCTGCCATTTTATCATGTGAAGATTTGGTTTCTATACTGATAAGCATTTTGATTTTAGGGTTATGCACTGATTGAATATGAGCGCTCTGAGGAAGCTCGGAATGCAATAGAGAATTTGAATGGATCTGTGCTTCTCACACAAACCATTTATGTTGATTGGGCTTTCAGCAGTGGACCTATCAATGAGTCAGTTAGAAGAAACAGGTAA
Protein sequence

>MS.gene21685.t1

MQGSAADGEALDFEPEEDDLMDEEGAVDAEASPPHPKLKSAITSVPKKTKGRGFRQDSDSSNRNSRLADSGFDTLTAEGGPGPQRSIEGWIILVTGVHEEAQDDDLQNAFGEYGDIKNLHLNLDRRTGFVKGYALIEYERSEEARNAIENLNGSVLLTQTIYVDWAFSSGPINESVRRNR