AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68735


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68735.t1 MTR_3g118110 100.000 132 0 0 1 132 64 195 1.56e-95 274
MS.gene68735.t1 MTR_3g109260 75.000 132 30 1 1 132 1 129 1.06e-70 209
MS.gene68735.t1 MTR_3g118110 87.209 86 11 0 1 86 64 149 3.40e-49 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68735.t1 AT1G02140 87.692 130 16 0 3 132 2 131 8.97e-85 244

Find 30 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTTACTACTTCCAAGAT+TGG 0.348725 3.2:+93127210 MS.gene68735:CDS
TAAAAGATGCGAAGGCCTTC+AGG 0.353524 3.2:-93127264 None:intergenic
CAGGAACTTGAGATTGTTAT+GGG 0.355218 3.2:+93127174 MS.gene68735:CDS
TCAGGAACTTGAGATTGTTA+TGG 0.374992 3.2:+93127173 MS.gene68735:CDS
CGACCAACACGGTCTGGTTC+AGG 0.414314 3.2:-93127150 None:intergenic
GAGATACTACGTTGGTCATA+AGG 0.416637 3.2:+93126844 MS.gene68735:CDS
ATCAACAAGAGAACCAATCT+TGG 0.427300 3.2:-93127223 None:intergenic
ACCAGACCGTGTTGGTCGTC+AGG 0.432239 3.2:+93127155 MS.gene68735:CDS
GTTGGTCATAAGGGTAAATT+CGG 0.435065 3.2:+93126854 MS.gene68735:CDS
TCCTGACGACCAACACGGTC+TGG 0.460919 3.2:-93127156 None:intergenic
ATAAACCTCCTTACGAATGA+TGG 0.480860 3.2:-93126954 None:intergenic
ACCAATCTTGGAAGTAGTAA+AGG 0.483775 3.2:-93127211 None:intergenic
AGATACTACGTTGGTCATAA+GGG 0.498454 3.2:+93126845 MS.gene68735:CDS
TGAAGGAGGATGATAACAAC+TGG 0.499238 3.2:+93127127 MS.gene68735:CDS
GCATAACGAAGCTTACCATC+TGG 0.517649 3.2:-93126905 None:intergenic
CGACACTCACGAAGAACAGC+AGG 0.558457 3.2:-93126983 None:intergenic
TCAACAACAACAGATTATGA+AGG 0.558662 3.2:+93127110 MS.gene68735:intron
GAGTTTGAGTTCCGTCCAGA+TGG 0.565284 3.2:+93126890 MS.gene68735:CDS
CAAGATAGTAAAAGATGCGA+AGG 0.565769 3.2:-93127272 None:intergenic
CAAAATGACAACACATCATG+GGG 0.567092 3.2:-93127593 None:intergenic
CGATACCATCATTCGTAAGG+AGG 0.567991 3.2:+93126949 MS.gene68735:CDS
AAACGATACCATCATTCGTA+AGG 0.579284 3.2:+93126946 MS.gene68735:CDS
CAAAGCAGTGCTGATCCTGA+AGG 0.593679 3.2:+93127249 MS.gene68735:CDS
AACGAAGCTTACCATCTGGA+CGG 0.634567 3.2:-93126901 None:intergenic
ACAACAACAGATTATGAAGG+AGG 0.642882 3.2:+93127113 MS.gene68735:intron
CAAGTTCCTGACGACCAACA+CGG 0.649649 3.2:-93127161 None:intergenic
TGGCCTGAACCAGACCGTGT+TGG 0.677826 3.2:+93127147 MS.gene68735:CDS
TCGTATTATTTCTGACAGCG+AGG 0.703758 3.2:+93127006 MS.gene68735:CDS
AGGAACTTGAGATTGTTATG+GGG 0.708397 3.2:+93127175 MS.gene68735:CDS
AAAATGACAACACATCATGG+GGG 0.747133 3.2:-93127592 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTATAAGCAATAATAAAAA+AGG - chr3.2:93127085-93127104 None:intergenic 15.0%
!! TAAAGTATATTTGTGAATTT+AGG - chr3.2:93127435-93127454 None:intergenic 15.0%
!! AATAATAAAAAAGGAAAAGG+GGG - chr3.2:93127076-93127095 None:intergenic 20.0%
!! AGCAATAATAAAAAAGGAAA+AGG - chr3.2:93127079-93127098 None:intergenic 20.0%
!! CAATAATAAAAAAGGAAAAG+GGG - chr3.2:93127077-93127096 None:intergenic 20.0%
!! GCAATAATAAAAAAGGAAAA+GGG - chr3.2:93127078-93127097 None:intergenic 20.0%
! ATAATAAAAAAGGAAAAGGG+GGG - chr3.2:93127075-93127094 None:intergenic 25.0%
! CACAAATATACTTTAGACAA+TGG + chr3.2:93127440-93127459 MS.gene68735:intron 25.0%
! CAGTAAAAAAATAACAGCAA+AGG - chr3.2:93127546-93127565 None:intergenic 25.0%
! CATATATTGATACTTTAGCT+GGG + chr3.2:93127466-93127485 MS.gene68735:intron 25.0%
! TATATTTGTGAATTTAGGCA+TGG - chr3.2:93127430-93127449 None:intergenic 25.0%
! TCATATATTGATACTTTAGC+TGG + chr3.2:93127465-93127484 MS.gene68735:intron 25.0%
! TTTCAATATAGCTGATGTAA+TGG + chr3.2:93127490-93127509 MS.gene68735:intron 25.0%
! TTTGGTATGCTTAAATTTGA+AGG - chr3.2:93127318-93127337 None:intergenic 25.0%
!! AATTTGAGTTAAGTTCATGA+AGG - chr3.2:93127370-93127389 None:intergenic 25.0%
!! TAAATTCGGACATGAATTTT+TGG + chr3.2:93126868-93126887 MS.gene68735:CDS 25.0%
AATGATATATGTAAGTTGCC+AGG - chr3.2:93127400-93127419 None:intergenic 30.0%
TAACTCAAATTAAACTGACC+TGG + chr3.2:93127379-93127398 MS.gene68735:intron 30.0%
TAATAAAAAAGGAAAAGGGG+GGG - chr3.2:93127074-93127093 None:intergenic 30.0%
TCAACAACAACAGATTATGA+AGG + chr3.2:93127110-93127129 MS.gene68735:intron 30.0%
! CATCTTTTACTATCTTGTTC+AGG + chr3.2:93127278-93127297 MS.gene68735:CDS 30.0%
! TTTTACTTGAGATACTACGT+TGG + chr3.2:93126836-93126855 MS.gene68735:CDS 30.0%
!! AGCAAAGGAATTCTAAAGTA+TGG - chr3.2:93127531-93127550 None:intergenic 30.0%
!! GATGGTATCGTTTTTGTAAT+TGG - chr3.2:93126939-93126958 None:intergenic 30.0%
!! GCAAAGGAATTCTAAAGTAT+GGG - chr3.2:93127530-93127549 None:intergenic 30.0%
AAACGATACCATCATTCGTA+AGG + chr3.2:93126946-93126965 MS.gene68735:CDS 35.0%
ACAACAACAGATTATGAAGG+AGG + chr3.2:93127113-93127132 MS.gene68735:intron 35.0%
ACCAATCTTGGAAGTAGTAA+AGG - chr3.2:93127214-93127233 None:intergenic 35.0%
AGATACTACGTTGGTCATAA+GGG + chr3.2:93126845-93126864 MS.gene68735:CDS 35.0%
AGGAACTTGAGATTGTTATG+GGG + chr3.2:93127175-93127194 MS.gene68735:CDS 35.0%
ATAAACCTCCTTACGAATGA+TGG - chr3.2:93126957-93126976 None:intergenic 35.0%
ATCAACAAGAGAACCAATCT+TGG - chr3.2:93127226-93127245 None:intergenic 35.0%
CAAGATAGTAAAAGATGCGA+AGG - chr3.2:93127275-93127294 None:intergenic 35.0%
CAGGAACTTGAGATTGTTAT+GGG + chr3.2:93127174-93127193 MS.gene68735:CDS 35.0%
GTTGGTCATAAGGGTAAATT+CGG + chr3.2:93126854-93126873 MS.gene68735:CDS 35.0%
TCAGGAACTTGAGATTGTTA+TGG + chr3.2:93127173-93127192 MS.gene68735:CDS 35.0%
TCCTTTACTACTTCCAAGAT+TGG + chr3.2:93127210-93127229 MS.gene68735:CDS 35.0%
TGTGAATTTAGGCATGGTAA+TGG - chr3.2:93127424-93127443 None:intergenic 35.0%
! TTTACTGTATTTCTGCAGAG+TGG + chr3.2:93127559-93127578 MS.gene68735:intron 35.0%
GAGATACTACGTTGGTCATA+AGG + chr3.2:93126844-93126863 MS.gene68735:CDS 40.0%
TGAAGGAGGATGATAACAAC+TGG + chr3.2:93127127-93127146 MS.gene68735:CDS 40.0%
! GCTTAGTGTTTGAGCAGTTT+TGG - chr3.2:93127336-93127355 None:intergenic 40.0%
!! TCGTATTATTTCTGACAGCG+AGG + chr3.2:93127006-93127025 MS.gene68735:CDS 40.0%
AACGAAGCTTACCATCTGGA+CGG - chr3.2:93126904-93126923 None:intergenic 45.0%
CGATACCATCATTCGTAAGG+AGG + chr3.2:93126949-93126968 MS.gene68735:CDS 45.0%
GAAAAGGGGGGGTGAAATTA+GGG - chr3.2:93127063-93127082 None:intergenic 45.0%
GCATAACGAAGCTTACCATC+TGG - chr3.2:93126908-93126927 None:intergenic 45.0%
TAAAAGATGCGAAGGCCTTC+AGG - chr3.2:93127267-93127286 None:intergenic 45.0%
CAAGTTCCTGACGACCAACA+CGG - chr3.2:93127164-93127183 None:intergenic 50.0%
GGAAAAGGGGGGGTGAAATT+AGG - chr3.2:93127064-93127083 None:intergenic 50.0%
GGGGGGTGAAATTAGGGTTA+GGG - chr3.2:93127057-93127076 None:intergenic 50.0%
! GAGTTTGAGTTCCGTCCAGA+TGG + chr3.2:93126890-93126909 MS.gene68735:CDS 50.0%
!! CAAAGCAGTGCTGATCCTGA+AGG + chr3.2:93127249-93127268 MS.gene68735:CDS 50.0%
CGACACTCACGAAGAACAGC+AGG - chr3.2:93126986-93127005 None:intergenic 55.0%
GGGGGGGTGAAATTAGGGTT+AGG - chr3.2:93127058-93127077 None:intergenic 55.0%
TCCTGACGACCAACACGGTC+TGG - chr3.2:93127159-93127178 None:intergenic 60.0%
TGGCCTGAACCAGACCGTGT+TGG + chr3.2:93127147-93127166 MS.gene68735:CDS 60.0%
! ACCAGACCGTGTTGGTCGTC+AGG + chr3.2:93127155-93127174 MS.gene68735:CDS 60.0%
! CGACCAACACGGTCTGGTTC+AGG - chr3.2:93127153-93127172 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 93126809 93127600 93126809 ID=MS.gene68735
chr3.2 mRNA 93126809 93127600 93126809 ID=MS.gene68735.t1;Parent=MS.gene68735
chr3.2 exon 93126809 93127027 93126809 ID=MS.gene68735.t1.exon1;Parent=MS.gene68735.t1
chr3.2 CDS 93126809 93127027 93126809 ID=cds.MS.gene68735.t1;Parent=MS.gene68735.t1
chr3.2 exon 93127123 93127299 93127123 ID=MS.gene68735.t1.exon2;Parent=MS.gene68735.t1
chr3.2 CDS 93127123 93127299 93127123 ID=cds.MS.gene68735.t1;Parent=MS.gene68735.t1
chr3.2 exon 93127577 93127600 93127577 ID=MS.gene68735.t1.exon3;Parent=MS.gene68735.t1
chr3.2 CDS 93127577 93127600 93127577 ID=cds.MS.gene68735.t1;Parent=MS.gene68735.t1
Gene Sequence

>MS.gene68735

ATGGGAAGCGAAGAAGAGAATGCAGAGTTTTACTTGAGATACTACGTTGGTCATAAGGGTAAATTCGGACATGAATTTTTGGAGTTTGAGTTCCGTCCAGATGGTAAGCTTCGTTATGCTAACAATTCCAATTACAAAAACGATACCATCATTCGTAAGGAGGTTTATCTTACTCCTGCTGTTCTTCGTGAGTGTCGTCGTATTATTTCTGACAGCGAGGTATTTTATTCTTTTCTTTTTACTAACCCTAACCCTAATTTCACCCCCCCTTTTCCTTTTTTATTATTGCTTATAACTCTATTCAACAACAACAGATTATGAAGGAGGATGATAACAACTGGCCTGAACCAGACCGTGTTGGTCGTCAGGAACTTGAGATTGTTATGGGGAATGAGCATATCTCCTTTACTACTTCCAAGATTGGTTCTCTTGTTGATGTTCAAAGCAGTGCTGATCCTGAAGGCCTTCGCATCTTTTACTATCTTGTTCAGGTCTGTCAGATTCTTCCTTCAAATTTAAGCATACCAAAACTGCTCAAACACTAAGCACAGATAATTGCCTTCATGAACTTAACTCAAATTAAACTGACCTGGCAACTTACATATATCATTACCATTACCATGCCTAAATTCACAAATATACTTTAGACAATGGTTTCATATATTGATACTTTAGCTGGGATTTCAATATAGCTGATGTAATGGTTTCAAAGTTTTAACCCATACTTTAGAATTCCTTTGCTGTTATTTTTTTACTGTATTTCTGCAGAGTGGTTTATATATTCCCCCATGA

Protein sequence

>MS.gene68735.t1

MGSEEENAEFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDTIIRKEVYLTPAVLRECRRIISDSEIMKEDDNNWPEPDRVGRQELEIVMGNEHISFTTSKIGSLVDVQSSADPEGLRIFYYLVQSGLYIPP