AlfalfaGEDB | MS.gene063086

M. sativa cultivar XinJiangDaYe / MS.gene063086

KEGG: K12877
GO: GO:0005575 GO:0005634 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0016070 GO:0032991 GO:0034641 GO:0035145 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0110165 GO:1901360
PFAM: PF02792.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063086.t1	KHN19112.1	88.2	153	14	1	1	149	1	153	5.90E-70	273.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063086.t1	O23676	84.7	137	21	0	5	141	4	140	2.7e-63	242.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063086.t1	A0A0B2QH55	88.2	153	14	1	1	149	1	153	4.3e-70	273.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene063086	0.825947	3.55E-54	-1.69E-46
MS.gene049341	MS.gene063086	0.811653	6.29E-51	-1.69E-46
MS.gene049821	MS.gene063086	0.819704	1.01E-52	-1.69E-46
MS.gene050020	MS.gene063086	0.826065	3.33E-54	-1.69E-46
MS.gene050384	MS.gene063086	0.812474	4.16E-51	-1.69E-46
MS.gene050386	MS.gene063086	0.807321	5.35E-50	-1.69E-46
MS.gene050689	MS.gene063086	0.84988	2.43E-60	-1.69E-46
MS.gene050807	MS.gene063086	0.827661	1.38E-54	-1.69E-46
MS.gene051243	MS.gene063086	0.83071	2.52E-55	-1.69E-46
MS.gene051328	MS.gene063086	0.840929	6.47E-58	-1.69E-46
MS.gene051343	MS.gene063086	0.807161	5.79E-50	-1.69E-46
MS.gene05134	MS.gene063086	0.811449	6.96E-51	-1.69E-46
MS.gene052335	MS.gene063086	0.815485	9.03E-52	-1.69E-46
MS.gene052947	MS.gene063086	0.804284	2.33E-49	-1.69E-46
MS.gene053058	MS.gene063086	0.819697	1.01E-52	-1.69E-46
MS.gene053563	MS.gene063086	0.808136	3.59E-50	-1.69E-46
MS.gene053581	MS.gene063086	0.819798	9.61E-53	-1.69E-46
MS.gene05364	MS.gene063086	0.823479	1.35E-53	-1.69E-46
MS.gene05462	MS.gene063086	0.836343	9.89E-57	-1.69E-46
MS.gene054657	MS.gene063086	0.803353	3.63E-49	-1.69E-46
MS.gene055468	MS.gene063086	0.802793	4.74E-49	-1.69E-46
MS.gene055512	MS.gene063086	0.811977	5.34E-51	-1.69E-46
MS.gene056215	MS.gene063086	0.810852	9.38E-51	-1.69E-46
MS.gene056580	MS.gene063086	0.80594	1.05E-49	-1.69E-46
MS.gene057060	MS.gene063086	0.809651	1.70E-50	-1.69E-46
MS.gene057287	MS.gene063086	0.81517	1.06E-51	-1.69E-46
MS.gene057694	MS.gene063086	0.818396	2.00E-52	-1.69E-46
MS.gene057705	MS.gene063086	0.839113	1.92E-57	-1.69E-46
MS.gene058216	MS.gene063086	0.803839	2.88E-49	-1.69E-46
MS.gene05978	MS.gene063086	0.840871	6.70E-58	-1.69E-46
MS.gene060429	MS.gene063086	0.805565	1.26E-49	-1.69E-46
MS.gene060587	MS.gene063086	0.822129	2.79E-53	-1.69E-46
MS.gene060917	MS.gene063086	0.803793	2.94E-49	-1.69E-46
MS.gene060918	MS.gene063086	0.807513	4.87E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene063086	MS.gene024706	PPI
MS.gene22282	MS.gene063086	PPI
MS.gene21685	MS.gene063086	PPI
MS.gene21943	MS.gene063086	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063086.t1	MTR_3g118110	100.000	132	0	0	1	132	64	195	9.66e-96	275
MS.gene063086.t1	MTR_3g109260	71.631	141	37	1	1	141	1	138	2.81e-71	211
MS.gene063086.t1	MTR_3g118110	87.209	86	11	0	1	86	64	149	2.67e-49	156

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063086.t1	AT1G02140	83.453	139	23	0	3	141	2	140	7.20e-85	245

Find 30 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTTAGTGTTTGAGCAGTTT+TGG	0.330451	3.3:-95863146	None:intergenic
TCCTTTACTACTTCCAAGAT+TGG	0.348725	3.3:+95863023	MS.gene063086:CDS
TAAAAGATGCGAAGGCCTTC+AGG	0.353524	3.3:-95863077	None:intergenic
CAGGAACTTGAGATTGTTAT+GGG	0.355218	3.3:+95862987	MS.gene063086:CDS
TCAGGAACTTGAGATTGTTA+TGG	0.374992	3.3:+95862986	MS.gene063086:CDS
CGACCAACACGGTCTGGTTC+AGG	0.414314	3.3:-95862963	None:intergenic
GAGATACTACGTTGGTCATA+AGG	0.416637	3.3:+95862654	MS.gene063086:CDS
ATCAACAAGAGAACCAATCT+TGG	0.427300	3.3:-95863036	None:intergenic
ACCAGACCGTGTTGGTCGTC+AGG	0.432239	3.3:+95862968	MS.gene063086:CDS
GTTGGTCATAAGGGTAAATT+CGG	0.435065	3.3:+95862664	MS.gene063086:CDS
TTTGGTATGCTTAAATTTGA+AGG	0.452213	3.3:-95863128	None:intergenic
TCCTGACGACCAACACGGTC+TGG	0.460919	3.3:-95862969	None:intergenic
ATAAACCTCCTTACGAATGA+TGG	0.480860	3.3:-95862764	None:intergenic
ACCAATCTTGGAAGTAGTAA+AGG	0.483775	3.3:-95863024	None:intergenic
AGATACTACGTTGGTCATAA+GGG	0.498454	3.3:+95862655	MS.gene063086:CDS
TGAAGGAGGATGATAACAAC+TGG	0.499238	3.3:+95862940	MS.gene063086:CDS
GCATAACGAAGCTTACCATC+TGG	0.517649	3.3:-95862715	None:intergenic
CGACACTCACGAAGAACAGC+AGG	0.558457	3.3:-95862793	None:intergenic
TCAACAACAACAGATTATGA+AGG	0.558662	3.3:+95862923	MS.gene063086:intron
GAGTTTGAGTTCCGTCCAGA+TGG	0.565284	3.3:+95862700	MS.gene063086:CDS
CAAGATAGTAAAAGATGCGA+AGG	0.565769	3.3:-95863085	None:intergenic
CGATACCATCATTCGTAAGG+AGG	0.567991	3.3:+95862759	MS.gene063086:CDS
AAACGATACCATCATTCGTA+AGG	0.579284	3.3:+95862756	MS.gene063086:CDS
CAAAGCAGTGCTGATCCTGA+AGG	0.593679	3.3:+95863062	MS.gene063086:CDS
AACGAAGCTTACCATCTGGA+CGG	0.634567	3.3:-95862711	None:intergenic
ACAACAACAGATTATGAAGG+AGG	0.642882	3.3:+95862926	MS.gene063086:intron
CAAGTTCCTGACGACCAACA+CGG	0.649649	3.3:-95862974	None:intergenic
TGGCCTGAACCAGACCGTGT+TGG	0.677826	3.3:+95862960	MS.gene063086:CDS
TCGTATTATTTCTGACAGCG+AGG	0.703758	3.3:+95862816	MS.gene063086:CDS
AGGAACTTGAGATTGTTATG+GGG	0.708397	3.3:+95862988	MS.gene063086:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTATAAGCAATAACAAAA+AGG	-	chr3.3:95862899-95862918	None:intergenic	20.0%
!	AAGCAATAACAAAAAGGAAA+AGG	-	chr3.3:95862893-95862912	None:intergenic	25.0%
!	AGCAATAACAAAAAGGAAAA+GGG	-	chr3.3:95862892-95862911	None:intergenic	25.0%
!	TTTGGTATGCTTAAATTTGA+AGG	-	chr3.3:95863131-95863150	None:intergenic	25.0%
!!	TAAATTCGGACATGAATTTT+TGG	+	chr3.3:95862678-95862697	MS.gene063086:CDS	25.0%
	AATAACAAAAAGGAAAAGGG+GGG	-	chr3.3:95862889-95862908	None:intergenic	30.0%
	CAATAACAAAAAGGAAAAGG+GGG	-	chr3.3:95862890-95862909	None:intergenic	30.0%
	GCAATAACAAAAAGGAAAAG+GGG	-	chr3.3:95862891-95862910	None:intergenic	30.0%
	TCAACAACAACAGATTATGA+AGG	+	chr3.3:95862923-95862942	MS.gene063086:intron	30.0%
!	CATCTTTTACTATCTTGTTC+AGG	+	chr3.3:95863091-95863110	MS.gene063086:CDS	30.0%
!	TTTTACTTGAGATACTACGT+TGG	+	chr3.3:95862646-95862665	MS.gene063086:CDS	30.0%
!!	GATGGTATCGTTTTTGTAAT+TGG	-	chr3.3:95862749-95862768	None:intergenic	30.0%
	AAACGATACCATCATTCGTA+AGG	+	chr3.3:95862756-95862775	MS.gene063086:CDS	35.0%
	ACAACAACAGATTATGAAGG+AGG	+	chr3.3:95862926-95862945	MS.gene063086:intron	35.0%
	ACCAATCTTGGAAGTAGTAA+AGG	-	chr3.3:95863027-95863046	None:intergenic	35.0%
	AGATACTACGTTGGTCATAA+GGG	+	chr3.3:95862655-95862674	MS.gene063086:CDS	35.0%
	AGGAACTTGAGATTGTTATG+GGG	+	chr3.3:95862988-95863007	MS.gene063086:CDS	35.0%
	ATAAACCTCCTTACGAATGA+TGG	-	chr3.3:95862767-95862786	None:intergenic	35.0%
	ATCAACAAGAGAACCAATCT+TGG	-	chr3.3:95863039-95863058	None:intergenic	35.0%
	CAAGATAGTAAAAGATGCGA+AGG	-	chr3.3:95863088-95863107	None:intergenic	35.0%
	CAGGAACTTGAGATTGTTAT+GGG	+	chr3.3:95862987-95863006	MS.gene063086:CDS	35.0%
	GTTGGTCATAAGGGTAAATT+CGG	+	chr3.3:95862664-95862683	MS.gene063086:CDS	35.0%
	TCAGGAACTTGAGATTGTTA+TGG	+	chr3.3:95862986-95863005	MS.gene063086:CDS	35.0%
	TCCTTTACTACTTCCAAGAT+TGG	+	chr3.3:95863023-95863042	MS.gene063086:CDS	35.0%
	GAGATACTACGTTGGTCATA+AGG	+	chr3.3:95862654-95862673	MS.gene063086:CDS	40.0%
	TGAAGGAGGATGATAACAAC+TGG	+	chr3.3:95862940-95862959	MS.gene063086:CDS	40.0%
!!	TCGTATTATTTCTGACAGCG+AGG	+	chr3.3:95862816-95862835	MS.gene063086:CDS	40.0%
	AACGAAGCTTACCATCTGGA+CGG	-	chr3.3:95862714-95862733	None:intergenic	45.0%
	AGGAAAAGGGGGGTGAAATT+AGG	-	chr3.3:95862879-95862898	None:intergenic	45.0%
	CGATACCATCATTCGTAAGG+AGG	+	chr3.3:95862759-95862778	MS.gene063086:CDS	45.0%
	GCATAACGAAGCTTACCATC+TGG	-	chr3.3:95862718-95862737	None:intergenic	45.0%
	GGAAAAGGGGGGTGAAATTA+GGG	-	chr3.3:95862878-95862897	None:intergenic	45.0%
	TAAAAGATGCGAAGGCCTTC+AGG	-	chr3.3:95863080-95863099	None:intergenic	45.0%
	AGGGGGGTGAAATTAGGGTT+AGG	-	chr3.3:95862873-95862892	None:intergenic	50.0%
	CAAGTTCCTGACGACCAACA+CGG	-	chr3.3:95862977-95862996	None:intergenic	50.0%
	GGGGGGTGAAATTAGGGTTA+GGG	-	chr3.3:95862872-95862891	None:intergenic	50.0%
!	GAGTTTGAGTTCCGTCCAGA+TGG	+	chr3.3:95862700-95862719	MS.gene063086:CDS	50.0%
!!	CAAAGCAGTGCTGATCCTGA+AGG	+	chr3.3:95863062-95863081	MS.gene063086:CDS	50.0%
	CGACACTCACGAAGAACAGC+AGG	-	chr3.3:95862796-95862815	None:intergenic	55.0%
	TCCTGACGACCAACACGGTC+TGG	-	chr3.3:95862972-95862991	None:intergenic	60.0%
	TGGCCTGAACCAGACCGTGT+TGG	+	chr3.3:95862960-95862979	MS.gene063086:CDS	60.0%
!	ACCAGACCGTGTTGGTCGTC+AGG	+	chr3.3:95862968-95862987	MS.gene063086:CDS	60.0%
!	CGACCAACACGGTCTGGTTC+AGG	-	chr3.3:95862966-95862985	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	95862619	95863166	95862619	ID=MS.gene063086
chr3.3	mRNA	95862619	95863166	95862619	ID=MS.gene063086.t1;Parent=MS.gene063086
chr3.3	exon	95862619	95862837	95862619	ID=MS.gene063086.t1.exon1;Parent=MS.gene063086.t1
chr3.3	CDS	95862619	95862837	95862619	ID=cds.MS.gene063086.t1;Parent=MS.gene063086.t1
chr3.3	exon	95862936	95863166	95862936	ID=MS.gene063086.t1.exon2;Parent=MS.gene063086.t1
chr3.3	CDS	95862936	95863166	95862936	ID=cds.MS.gene063086.t1;Parent=MS.gene063086.t1

Gene Sequence

>MS.gene063086

ATGGGAAGCGAAGAAGAGAATGCAGAGTTTTACTTGAGATACTACGTTGGTCATAAGGGTAAATTCGGACATGAATTTTTGGAGTTTGAGTTCCGTCCAGATGGTAAGCTTCGTTATGCTAACAATTCCAATTACAAAAACGATACCATCATTCGTAAGGAGGTTTATCTTACTCCTGCTGTTCTTCGTGAGTGTCGTCGTATTATTTCTGACAGCGAGGTATTTTATTCTTTTCTTTTCTTTTTACTAACCCTAACCCTAATTTCACCCCCCTTTTCCTTTTTGTTATTGCTTATAACTCTATTCAACAACAACAGATTATGAAGGAGGATGATAACAACTGGCCTGAACCAGACCGTGTTGGTCGTCAGGAACTTGAGATTGTTATGGGGAATGAGCATATCTCCTTTACTACTTCCAAGATTGGTTCTCTTGTTGATGTTCAAAGCAGTGCTGATCCTGAAGGCCTTCGCATCTTTTACTATCTTGTTCAGGTCTGTCAGATTCTTCCTTCAAATTTAAGCATACCAAAACTGCTCAAACACTAA

Protein sequence

>MS.gene063086.t1

MGSEEENAEFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDTIIRKEVYLTPAVLRECRRIISDSEIMKEDDNNWPEPDRVGRQELEIVMGNEHISFTTSKIGSLVDVQSSADPEGLRIFYYLVQVCQILPSNLSIPKLLKH