Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23100.t1 | XP_003626145.1 | 98 | 455 | 7 | 2 | 1 | 453 | 1 | 455 | 3.40E-262 | 913.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23100.t1 | Q3E6S9 | 71.9 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 3.5e-191 | 669.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23100.t1 | G7KWF1 | 98.0 | 455 | 7 | 2 | 1 | 453 | 1 | 455 | 2.5e-262 | 913.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050794 | MS.gene23100 | 0.988461 | 4.15E-174 | -1.69E-46 |
| MS.gene050796 | MS.gene23100 | 0.996805 | 1.78E-232 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07363 | MS.gene23100 | PPI |
| MS.gene005429 | MS.gene23100 | PPI |
| MS.gene20907 | MS.gene23100 | PPI |
| MS.gene20906 | MS.gene23100 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23100.t1 | MTR_7g111540 | 98.022 | 455 | 7 | 2 | 1 | 453 | 1 | 455 | 0.0 | 930 |
| MS.gene23100.t1 | MTR_1g484920 | 71.239 | 452 | 115 | 7 | 4 | 452 | 5 | 444 | 0.0 | 670 |
| MS.gene23100.t1 | MTR_1g484920 | 64.000 | 450 | 108 | 7 | 4 | 452 | 5 | 401 | 0.0 | 583 |
| MS.gene23100.t1 | MTR_1g086070 | 60.225 | 445 | 166 | 4 | 16 | 453 | 9 | 449 | 0.0 | 561 |
| MS.gene23100.t1 | MTR_3g083980 | 23.887 | 247 | 158 | 9 | 52 | 286 | 79 | 307 | 4.25e-11 | 64.7 |
| MS.gene23100.t1 | MTR_3g083980 | 23.887 | 247 | 158 | 9 | 52 | 286 | 79 | 307 | 7.17e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23100.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 659 |
| MS.gene23100.t1 | AT5G26600 | 71.860 | 430 | 121 | 0 | 23 | 452 | 45 | 474 | 0.0 | 659 |
| MS.gene23100.t1 | AT3G62130 | 59.465 | 449 | 171 | 3 | 10 | 451 | 7 | 451 | 0.0 | 583 |
| MS.gene23100.t1 | AT3G62130 | 59.465 | 449 | 171 | 3 | 10 | 451 | 7 | 451 | 0.0 | 583 |
Find 0 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGGAAGAAGATAGTTT+AGG | + | 50595:421-440 | None:intergenic | 25.0% |
| ! | ACTAAAAAGAATCCTAAAAC+TGG | - | 50595:1168-1187 | MS.gene23100:CDS | 25.0% |
| ! | TTCTTATCAGGTTTACAATA+AGG | - | 50595:1605-1624 | MS.gene23100:CDS | 25.0% |
| !! | ATACTAGTAATTTGCATAAG+TGG | - | 50595:1112-1131 | MS.gene23100:CDS | 25.0% |
| !!! | AAGATAGTTTAGGTTTTTTG+GGG | + | 50595:411-430 | None:intergenic | 25.0% |
| !!! | AGAAGATAGTTTAGGTTTTT+TGG | + | 50595:413-432 | None:intergenic | 25.0% |
| !!! | GAAGATAGTTTAGGTTTTTT+GGG | + | 50595:412-431 | None:intergenic | 25.0% |
| !!! | TTAGAAAAGCTTTGGAAAAA+GGG | - | 50595:902-921 | MS.gene23100:CDS | 25.0% |
| !!! | TTTAGAAAAGCTTTGGAAAA+AGG | - | 50595:901-920 | MS.gene23100:CDS | 25.0% |
| AAAAAGAATCCTAAAACTGG+TGG | - | 50595:1171-1190 | MS.gene23100:CDS | 30.0% | |
| ATACTAGCACACATATGTTT+CGG | + | 50595:1426-1445 | None:intergenic | 30.0% | |
| CTTTGTTGAATTTACCTTCA+CGG | + | 50595:741-760 | None:intergenic | 30.0% | |
| GATGTTGATATGCAAGATAT+TGG | - | 50595:1078-1097 | MS.gene23100:CDS | 30.0% | |
| GTTATGCTTCATTATGCTTA+CGG | - | 50595:772-791 | MS.gene23100:CDS | 30.0% | |
| TGGGAAGAAAATTAGATTAG+CGG | - | 50595:933-952 | MS.gene23100:CDS | 30.0% | |
| TTGAGGACTATTACAAGTTT+CGG | - | 50595:1628-1647 | MS.gene23100:CDS | 30.0% | |
| ! | GATTTTGTGAATAGGTTTGA+AGG | - | 50595:1309-1328 | MS.gene23100:CDS | 30.0% |
| !!! | AGATAGTTTAGGTTTTTTGG+GGG | + | 50595:410-429 | None:intergenic | 30.0% |
| AAGGGATTATAGTGCTCAAT+TGG | - | 50595:1269-1288 | MS.gene23100:CDS | 35.0% | |
| ATCTGAATCAATTCCTTCAC+AGG | + | 50595:997-1016 | None:intergenic | 35.0% | |
| CATGAAGCTGTTATTGAGAT+GGG | - | 50595:1360-1379 | MS.gene23100:CDS | 35.0% | |
| CGTGAAGGTAAATTCAACAA+AGG | - | 50595:739-758 | MS.gene23100:CDS | 35.0% | |
| CTAGGCGGTCTATAATATAT+AGG | + | 50595:1546-1565 | None:intergenic | 35.0% | |
| GAAACATATGTGTGCTAGTA+TGG | - | 50595:1425-1444 | MS.gene23100:CDS | 35.0% | |
| GACTGAGTTTAGAAAAGCTT+TGG | - | 50595:894-913 | MS.gene23100:CDS | 35.0% | |
| GTATGCAAGGATTTCTTATC+AGG | - | 50595:1593-1612 | MS.gene23100:CDS | 35.0% | |
| TATGTGTGCTAGTATGGTTA+TGG | - | 50595:1431-1450 | MS.gene23100:CDS | 35.0% | |
| TCAGGTTTACAATAAGGTTG+AGG | - | 50595:1611-1630 | MS.gene23100:CDS | 35.0% | |
| TCATGAAGCTGTTATTGAGA+TGG | - | 50595:1359-1378 | MS.gene23100:CDS | 35.0% | |
| TGAGGACTATTACAAGTTTC+GGG | - | 50595:1629-1648 | MS.gene23100:CDS | 35.0% | |
| TTGGAAAAAGGGAAAGTTGA+TGG | - | 50595:913-932 | MS.gene23100:CDS | 35.0% | |
| TTGTGAATAGGTTTGAAGGA+GGG | - | 50595:1313-1332 | MS.gene23100:CDS | 35.0% | |
| TTTGTGAATAGGTTTGAAGG+AGG | - | 50595:1312-1331 | MS.gene23100:CDS | 35.0% | |
| ! | ACACATTTGAGGGATGTTTT+TGG | - | 50595:1510-1529 | MS.gene23100:CDS | 35.0% |
| ! | ATTGATTCAGATTTGCAGAG+AGG | - | 50595:1005-1024 | MS.gene23100:CDS | 35.0% |
| ! | TGGAAAAAGGGAAAGTTGAT+GGG | - | 50595:914-933 | MS.gene23100:CDS | 35.0% |
| ! | TTAACCAACTGGTGGATAAT+GGG | - | 50595:1658-1677 | MS.gene23100:CDS | 35.0% |
| ! | TTCGGATTCAATTTCTGAAG+CGG | + | 50595:449-468 | None:intergenic | 35.0% |
| !!! | AGGCTTTGGATTTTGTGAAT+AGG | - | 50595:1301-1320 | MS.gene23100:CDS | 35.0% |
| !!! | CTAGGTTTTGGATAATTGAG+CGG | + | 50595:630-649 | None:intergenic | 35.0% |
| !!! | TAGGTTTTGGATAATTGAGC+GGG | + | 50595:629-648 | None:intergenic | 35.0% |
| !!! | TTGGTTTGCCTACTTGTTTT+GGG | - | 50595:1454-1473 | MS.gene23100:CDS | 35.0% |
| AAGAATCCTAAAACTGGTGG+TGG | - | 50595:1174-1193 | MS.gene23100:CDS | 40.0% | |
| ATTCAGATTTGCAGAGAGGA+AGG | - | 50595:1009-1028 | MS.gene23100:CDS | 40.0% | |
| ATTCTGATGCACTTAAGCTG+AGG | - | 50595:1487-1506 | MS.gene23100:CDS | 40.0% | |
| CTTCTAACCCCAAAACAAGT+AGG | + | 50595:1465-1484 | None:intergenic | 40.0% | |
| GGATTATAGTGCTCAATTGG+TGG | - | 50595:1272-1291 | MS.gene23100:CDS | 40.0% | |
| GGATTGAAGGGATTAAGAAG+AGG | - | 50595:1334-1353 | MS.gene23100:CDS | 40.0% | |
| GTATACAAAAACGCGATCGA+AGG | + | 50595:1150-1169 | None:intergenic | 40.0% | |
| GTGGTTGAAGTAGAAGTGAT+CGG | + | 50595:584-603 | None:intergenic | 40.0% | |
| GTGTCTCATGAGTATGGAAA+TGG | - | 50595:1216-1235 | MS.gene23100:CDS | 40.0% | |
| TAAGCTGAGGACACATTTGA+GGG | - | 50595:1500-1519 | MS.gene23100:CDS | 40.0% | |
| TATTATAGACCGCCTAGAGA+TGG | - | 50595:1549-1568 | MS.gene23100:CDS | 40.0% | |
| TATTGAGATGGGAGAGATGT+TGG | - | 50595:1371-1390 | MS.gene23100:CDS | 40.0% | |
| TGCTGTTAAGAAATCCATGG+AGG | - | 50595:795-814 | MS.gene23100:CDS | 40.0% | |
| TGGTTGAAGTAGAAGTGATC+GGG | + | 50595:583-602 | None:intergenic | 40.0% | |
| TGTGCTAGTATGGTTATGGT+TGG | - | 50595:1435-1454 | MS.gene23100:CDS | 40.0% | |
| TTAAGCTGAGGACACATTTG+AGG | - | 50595:1499-1518 | MS.gene23100:CDS | 40.0% | |
| TTCACAAAATCCAAAGCCTC+AGG | + | 50595:1300-1319 | None:intergenic | 40.0% | |
| TTCACAGGAATCACAACACA+AGG | + | 50595:982-1001 | None:intergenic | 40.0% | |
| TTGTGTTGTGATTCCTGTGA+AGG | - | 50595:981-1000 | MS.gene23100:CDS | 40.0% | |
| ! | AAATCACCACCACCAGTTTT+AGG | + | 50595:1183-1202 | None:intergenic | 40.0% |
| ! | AGAGATGTTGGTGAAAGCTT+GGG | - | 50595:1383-1402 | MS.gene23100:CDS | 40.0% |
| ! | GTTAACCAACTGGTGGATAA+TGG | - | 50595:1657-1676 | MS.gene23100:CDS | 40.0% |
| ! | TGAAGTTCCGTTACCTTTTC+CGG | - | 50595:849-868 | MS.gene23100:CDS | 40.0% |
| !! | GTATGGAAATGGATTAGCTG+TGG | - | 50595:1227-1246 | MS.gene23100:CDS | 40.0% |
| !! | GTGGTGATTGTTTTTCTCAG+AGG | + | 50595:353-372 | None:intergenic | 40.0% |
| !! | TGATGTCTTTAGCGTTGACT+AGG | + | 50595:648-667 | None:intergenic | 40.0% |
| !! | TTCTGAAGCGGTGATGAAAT+TGG | + | 50595:437-456 | None:intergenic | 40.0% |
| !!! | AGTTTAGGTTTTTTGGGGGT+GGG | + | 50595:406-425 | None:intergenic | 40.0% |
| !!! | CTTTAGCGTTGACTAGGTTT+TGG | + | 50595:642-661 | None:intergenic | 40.0% |
| !!! | GTTGGTTTGCCTACTTGTTT+TGG | - | 50595:1453-1472 | MS.gene23100:CDS | 40.0% |
| !!! | TAGTTTAGGTTTTTTGGGGG+TGG | + | 50595:407-426 | None:intergenic | 40.0% |
| !!! | TGGTTTGCCTACTTGTTTTG+GGG | - | 50595:1455-1474 | MS.gene23100:CDS | 40.0% |
| AAAGCTTGGGGAACTCATCT+TGG | - | 50595:1396-1415 | MS.gene23100:CDS | 45.0% | |
| AAGCTTGGGGAACTCATCTT+GGG | - | 50595:1397-1416 | MS.gene23100:CDS | 45.0% | |
| AATTGAGCGGGAGTTGTTGA+TGG | + | 50595:617-636 | None:intergenic | 45.0% | |
| AGGTTTGAAGGAGGGATTGA+AGG | - | 50595:1321-1340 | MS.gene23100:CDS | 45.0% | |
| ATCTCATCGTTCGAACTCAC+CGG | + | 50595:871-890 | None:intergenic | 45.0% | |
| CAAACCCATTATCCACCAGT+TGG | + | 50595:1665-1684 | None:intergenic | 45.0% | |
| CCATACTCATGAGACACAAC+AGG | + | 50595:1213-1232 | None:intergenic | 45.0% | |
| CCTGTTGTGTCTCATGAGTA+TGG | - | 50595:1210-1229 | MS.gene23100:CDS | 45.0% | |
| CGTTCAATCCCAGACATCAA+CGG | - | 50595:373-392 | MS.gene23100:CDS | 45.0% | |
| GAACTCACCGGAAAAGGTAA+CGG | + | 50595:859-878 | None:intergenic | 45.0% | |
| GGATCAACTTCTCCATCTCT+AGG | + | 50595:1564-1583 | None:intergenic | 45.0% | |
| GGTGTGTTGAAGAACGATAG+CGG | + | 50595:707-726 | None:intergenic | 45.0% | |
| GGTTTGAAGGAGGGATTGAA+GGG | - | 50595:1322-1341 | MS.gene23100:CDS | 45.0% | |
| GTTGATGCTGCTCATGCAAT+TGG | - | 50595:1048-1067 | MS.gene23100:CDS | 45.0% | |
| TACAAAAACGCGATCGAAGG+TGG | + | 50595:1147-1166 | None:intergenic | 45.0% | |
| TCAACTTCTCCATCTCTAGG+CGG | + | 50595:1561-1580 | None:intergenic | 45.0% | |
| ! | CGTTGATGTCTGGGATTGAA+CGG | + | 50595:375-394 | None:intergenic | 45.0% |
| ! | GAGAGATGTTGGTGAAAGCT+TGG | - | 50595:1382-1401 | MS.gene23100:CDS | 45.0% |
| ! | GAGATGTTGGTGAAAGCTTG+GGG | - | 50595:1384-1403 | MS.gene23100:CDS | 45.0% |
| ! | TGGAGAAGTTGATCCTGTGA+CGG | - | 50595:1569-1588 | MS.gene23100:CDS | 45.0% |
| !! | AAAGTGCTTGGATTGGCACA+AGG | - | 50595:1250-1269 | MS.gene23100:CDS | 45.0% |
| !! | AAGTGCTTGGATTGGCACAA+GGG | - | 50595:1251-1270 | MS.gene23100:CDS | 45.0% |
| !! | CGGTGCTGTTAAGAAATCCA+TGG | - | 50595:792-811 | MS.gene23100:CDS | 45.0% |
| !! | GATTAGCTGTGGAAAGTGCT+TGG | - | 50595:1238-1257 | MS.gene23100:CDS | 45.0% |
| !!! | GTTTAGGTTTTTTGGGGGTG+GGG | + | 50595:405-424 | None:intergenic | 45.0% |
| ACCTTCACGGAAACACCAAG+CGG | + | 50595:728-747 | None:intergenic | 50.0% | |
| CGCATTAACAACGGCAGCTT+TGG | - | 50595:496-515 | MS.gene23100:CDS | 50.0% | |
| GCCGTTGCTCGCATTAACAA+CGG | - | 50595:487-506 | MS.gene23100:CDS | 50.0% | |
| GCCGTTGTTAATGCGAGCAA+CGG | + | 50595:491-510 | None:intergenic | 50.0% | |
| GGATGCTGTTAACCAACTGG+TGG | - | 50595:1650-1669 | MS.gene23100:CDS | 50.0% | |
| GTTGTTAATGCGAGCAACGG+CGG | + | 50595:488-507 | None:intergenic | 50.0% | |
| TCCTCTCCGCTCAACAACAA+TGG | - | 50595:536-555 | MS.gene23100:CDS | 50.0% | |
| TCGGGATGCTGTTAACCAAC+TGG | - | 50595:1647-1666 | MS.gene23100:CDS | 50.0% | |
| TCGTTCGAACTCACCGGAAA+AGG | + | 50595:865-884 | None:intergenic | 50.0% | |
| TCGTTCTTCAACACACCGCT+TGG | - | 50595:710-729 | MS.gene23100:CDS | 50.0% | |
| ! | ATTGGTGGTTCCTGAGGCTT+TGG | - | 50595:1287-1306 | MS.gene23100:CDS | 50.0% |
| ! | GAGAAGTTGATCCTGTGACG+GGG | - | 50595:1571-1590 | MS.gene23100:CDS | 50.0% |
| ! | GAGCTGCCATTGTTGTTGAG+CGG | + | 50595:545-564 | None:intergenic | 50.0% |
| ! | GGAGAAGTTGATCCTGTGAC+GGG | - | 50595:1570-1589 | MS.gene23100:CDS | 50.0% |
| ! | TGATGTCTGGGATTGAACGG+TGG | + | 50595:372-391 | None:intergenic | 50.0% |
| ! | TGCTCAATTGGTGGTTCCTG+AGG | - | 50595:1281-1300 | MS.gene23100:CDS | 50.0% |
| ! | TTGAAGAACGATAGCGGCAG+CGG | + | 50595:701-720 | None:intergenic | 50.0% |
| ! | TTGTTGTTGAGCGGAGAGGA+TGG | + | 50595:536-555 | None:intergenic | 50.0% |
| !! | AGTTGTTGATGGCGGGTTTG+AGG | + | 50595:606-625 | None:intergenic | 50.0% |
| !! | CAGAGAGGAAGGTGTTGAAC+AGG | - | 50595:1020-1039 | MS.gene23100:CDS | 50.0% |
| !! | GCTGTGGAAAGTGCTTGGAT+TGG | - | 50595:1243-1262 | MS.gene23100:CDS | 50.0% |
| ACGAGTGACGTAAGCCTCCA+TGG | + | 50595:812-831 | None:intergenic | 55.0% | |
| ATCCTTGCATACCCCGTCAC+AGG | + | 50595:1585-1604 | None:intergenic | 55.0% | |
| TTGTTGAGCGGAGAGGATGG+AGG | + | 50595:533-552 | None:intergenic | 55.0% | |
| ! | ATCCTGTGACGGGGTATGCA+AGG | - | 50595:1580-1599 | MS.gene23100:CDS | 55.0% |
| ! | CTTACGTCACTCGTGCTGGT+GGG | - | 50595:818-837 | MS.gene23100:CDS | 55.0% |
| ! | GCCATTGTTGTTGAGCGGAG+AGG | + | 50595:540-559 | None:intergenic | 55.0% |
| ! | GGTGTGTGCCGTTGATGTCT+GGG | + | 50595:384-403 | None:intergenic | 55.0% |
| ! | TGAGCGGGAGTTGTTGATGG+CGG | + | 50595:614-633 | None:intergenic | 55.0% |
| !! | ACCGCTTGGTGTTTCCGTGA+AGG | - | 50595:724-743 | MS.gene23100:CDS | 55.0% |
| !! | TGTTGATGGCGGGTTTGAGG+TGG | + | 50595:603-622 | None:intergenic | 55.0% |
| GAGGCTTACGTCACTCGTGC+TGG | - | 50595:814-833 | MS.gene23100:CDS | 60.0% | |
| GCTTACGTCACTCGTGCTGG+TGG | - | 50595:817-836 | MS.gene23100:CDS | 60.0% | |
| GGAGAGGATGGAGGAAGGAC+AGG | + | 50595:524-543 | None:intergenic | 60.0% | |
| GGCGTCGTGGTGGCTGAATT+CGG | + | 50595:467-486 | None:intergenic | 60.0% | |
| TGAGCGGAGAGGATGGAGGA+AGG | + | 50595:529-548 | None:intergenic | 60.0% | |
| ! | AAGAACGATAGCGGCAGCGG+TGG | + | 50595:698-717 | None:intergenic | 60.0% |
| ! | GGGTGTGTGCCGTTGATGTC+TGG | + | 50595:385-404 | None:intergenic | 60.0% |
| !! | GAGCGGGAGTTGTTGATGGC+GGG | + | 50595:613-632 | None:intergenic | 60.0% |
| ! | AACGATAGCGGCAGCGGTGG+TGG | + | 50595:695-714 | None:intergenic | 65.0% |
| GAGCAACGGCGGCGTCGTGG+TGG | + | 50595:477-496 | None:intergenic | 75.0% | |
| TGCGAGCAACGGCGGCGTCG+TGG | + | 50595:480-499 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50595 | gene | 346 | 1707 | 346 | ID=MS.gene23100 |
| 50595 | mRNA | 346 | 1707 | 346 | ID=MS.gene23100.t1;Parent=MS.gene23100 |
| 50595 | exon | 346 | 1707 | 346 | ID=MS.gene23100.t1.exon1;Parent=MS.gene23100.t1 |
| 50595 | CDS | 346 | 1707 | 346 | ID=cds.MS.gene23100.t1;Parent=MS.gene23100.t1 |
| Gene Sequence |
| Protein sequence |
