Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25137.t1 | XP_003627277.1 | 95.2 | 209 | 10 | 0 | 1 | 209 | 1 | 209 | 2.50E-106 | 394.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25137.t1 | Q9ZRA4 | 58.5 | 207 | 84 | 2 | 3 | 207 | 1 | 207 | 3.5e-61 | 236.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25137.t1 | G7L6U9 | 95.2 | 209 | 10 | 0 | 1 | 209 | 1 | 209 | 1.8e-106 | 394.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050381 | MS.gene25137 | 0.810739 | 9.93E-51 | -1.69E-46 |
| MS.gene050809 | MS.gene25137 | 0.803287 | 3.75E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene25137 | -0.803593 | 3.24E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene25137 | -0.804927 | 1.71E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene25137 | -0.802343 | 5.87E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene25137 | -0.802141 | 6.46E-49 | -1.69E-46 |
| MS.gene052825 | MS.gene25137 | -0.804393 | 2.21E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene25137 | -0.801587 | 8.39E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene25137 | 0.823592 | 1.27E-53 | -1.69E-46 |
| MS.gene054742 | MS.gene25137 | 0.801416 | 9.10E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene25137 | -0.802652 | 5.07E-49 | -1.69E-46 |
| MS.gene056330 | MS.gene25137 | 0.815851 | 7.48E-52 | -1.69E-46 |
| MS.gene056920 | MS.gene25137 | 0.801641 | 8.18E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene25137 | 0.809501 | 1.83E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene25137 | 0.810703 | 1.01E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene25137 | -0.814348 | 1.61E-51 | -1.69E-46 |
| MS.gene058416 | MS.gene25137 | 0.809441 | 1.89E-50 | -1.69E-46 |
| MS.gene060405 | MS.gene25137 | 0.827141 | 1.84E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25137.t1 | MTR_8g020630 | 95.215 | 209 | 10 | 0 | 1 | 209 | 1 | 209 | 6.42e-145 | 402 |
| MS.gene25137.t1 | MTR_8g020650 | 94.737 | 209 | 11 | 0 | 1 | 209 | 1 | 209 | 2.97e-137 | 382 |
| MS.gene25137.t1 | MTR_8g020670 | 88.517 | 209 | 24 | 0 | 1 | 209 | 1 | 209 | 6.21e-134 | 374 |
| MS.gene25137.t1 | MTR_2g044040 | 73.684 | 209 | 55 | 0 | 1 | 209 | 1 | 209 | 2.27e-111 | 317 |
| MS.gene25137.t1 | MTR_0284s0010 | 73.684 | 209 | 55 | 0 | 1 | 209 | 1 | 209 | 4.09e-109 | 311 |
| MS.gene25137.t1 | MTR_2g045100 | 70.813 | 209 | 61 | 0 | 1 | 209 | 1 | 209 | 8.04e-107 | 306 |
| MS.gene25137.t1 | MTR_2g036120 | 70.531 | 207 | 61 | 0 | 1 | 207 | 1 | 207 | 1.93e-105 | 302 |
| MS.gene25137.t1 | MTR_3g019710 | 70.335 | 209 | 62 | 0 | 1 | 209 | 1 | 209 | 2.17e-104 | 300 |
| MS.gene25137.t1 | MTR_5g031460 | 68.421 | 209 | 66 | 0 | 1 | 209 | 1 | 209 | 6.37e-101 | 291 |
| MS.gene25137.t1 | MTR_3g019650 | 68.421 | 209 | 65 | 1 | 1 | 209 | 1 | 208 | 3.14e-99 | 286 |
| MS.gene25137.t1 | MTR_3g019680 | 68.421 | 209 | 65 | 1 | 1 | 209 | 1 | 208 | 1.09e-98 | 285 |
| MS.gene25137.t1 | MTR_7g011340 | 70.335 | 209 | 62 | 0 | 1 | 209 | 1 | 209 | 7.12e-98 | 283 |
| MS.gene25137.t1 | MTR_3g019810 | 64.851 | 202 | 65 | 1 | 1 | 202 | 107 | 302 | 4.43e-84 | 252 |
| MS.gene25137.t1 | MTR_4g010340 | 58.454 | 207 | 84 | 2 | 3 | 209 | 1 | 205 | 1.51e-83 | 246 |
| MS.gene25137.t1 | MTR_8g020590 | 57.895 | 209 | 85 | 3 | 3 | 209 | 1 | 208 | 1.69e-81 | 241 |
| MS.gene25137.t1 | MTR_2g019780 | 56.279 | 215 | 85 | 3 | 1 | 209 | 1 | 212 | 2.80e-80 | 238 |
| MS.gene25137.t1 | MTR_3g073170 | 55.869 | 213 | 87 | 3 | 1 | 209 | 1 | 210 | 3.38e-79 | 236 |
| MS.gene25137.t1 | MTR_2g041550 | 55.981 | 209 | 88 | 3 | 3 | 209 | 4 | 210 | 2.45e-78 | 233 |
| MS.gene25137.t1 | MTR_8g020760 | 55.924 | 211 | 91 | 2 | 1 | 209 | 1 | 211 | 9.18e-78 | 232 |
| MS.gene25137.t1 | MTR_2g041580 | 54.976 | 211 | 92 | 3 | 1 | 209 | 1 | 210 | 2.71e-77 | 231 |
| MS.gene25137.t1 | MTR_2g041570 | 54.502 | 211 | 93 | 3 | 1 | 209 | 1 | 210 | 7.65e-77 | 230 |
| MS.gene25137.t1 | MTR_8g020770 | 54.245 | 212 | 94 | 3 | 1 | 209 | 1 | 212 | 3.16e-76 | 228 |
| MS.gene25137.t1 | MTR_8g020610 | 55.981 | 209 | 88 | 3 | 3 | 209 | 1 | 207 | 5.12e-76 | 228 |
| MS.gene25137.t1 | MTR_8g020750 | 53.302 | 212 | 96 | 3 | 1 | 209 | 1 | 212 | 1.77e-72 | 219 |
| MS.gene25137.t1 | MTR_8g020763 | 57.143 | 182 | 77 | 1 | 29 | 209 | 9 | 190 | 3.04e-71 | 215 |
| MS.gene25137.t1 | MTR_4g027415 | 73.239 | 142 | 38 | 0 | 1 | 142 | 1 | 142 | 1.23e-64 | 196 |
| MS.gene25137.t1 | MTR_2g072560 | 66.957 | 115 | 38 | 0 | 1 | 115 | 1 | 115 | 4.92e-52 | 164 |
| MS.gene25137.t1 | MTR_3g019610 | 70.755 | 106 | 31 | 0 | 1 | 106 | 1 | 106 | 5.18e-49 | 155 |
| MS.gene25137.t1 | MTR_7g111240 | 37.500 | 192 | 116 | 3 | 21 | 208 | 23 | 214 | 1.65e-36 | 127 |
| MS.gene25137.t1 | MTR_1g112200 | 36.408 | 206 | 122 | 6 | 3 | 205 | 8 | 207 | 2.62e-36 | 126 |
| MS.gene25137.t1 | MTR_1g085140 | 35.354 | 198 | 124 | 3 | 15 | 208 | 21 | 218 | 6.62e-36 | 125 |
| MS.gene25137.t1 | MTR_7g111240 | 37.500 | 192 | 113 | 4 | 21 | 208 | 23 | 211 | 1.34e-35 | 125 |
| MS.gene25137.t1 | MTR_1g085500 | 34.450 | 209 | 131 | 4 | 6 | 208 | 9 | 217 | 2.62e-35 | 124 |
| MS.gene25137.t1 | MTR_2g030855 | 35.859 | 198 | 118 | 4 | 14 | 205 | 18 | 212 | 3.64e-34 | 121 |
| MS.gene25137.t1 | MTR_2g031270 | 36.649 | 191 | 112 | 4 | 21 | 205 | 26 | 213 | 4.49e-34 | 121 |
| MS.gene25137.t1 | MTR_1g060120 | 33.168 | 202 | 127 | 2 | 12 | 205 | 20 | 221 | 1.04e-33 | 120 |
| MS.gene25137.t1 | MTR_1g079490 | 34.597 | 211 | 130 | 3 | 3 | 205 | 7 | 217 | 2.30e-33 | 119 |
| MS.gene25137.t1 | MTR_6g005330 | 37.500 | 216 | 123 | 5 | 1 | 205 | 1 | 215 | 5.45e-33 | 118 |
| MS.gene25137.t1 | MTR_4g017050 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 7.38e-33 | 118 |
| MS.gene25137.t1 | MTR_5g046410 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 1.02e-32 | 117 |
| MS.gene25137.t1 | MTR_5g046430 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 1.02e-32 | 117 |
| MS.gene25137.t1 | MTR_4g017030 | 36.530 | 219 | 120 | 7 | 1 | 204 | 1 | 215 | 1.02e-32 | 117 |
| MS.gene25137.t1 | MTR_6g005310 | 38.384 | 198 | 114 | 4 | 15 | 205 | 19 | 215 | 1.66e-32 | 117 |
| MS.gene25137.t1 | MTR_2g019250 | 36.073 | 219 | 121 | 7 | 1 | 204 | 1 | 215 | 2.20e-32 | 117 |
| MS.gene25137.t1 | MTR_6g005360 | 38.384 | 198 | 114 | 4 | 15 | 205 | 19 | 215 | 2.47e-32 | 116 |
| MS.gene25137.t1 | MTR_4g017040 | 35.484 | 217 | 125 | 6 | 1 | 204 | 1 | 215 | 4.18e-32 | 116 |
| MS.gene25137.t1 | MTR_6g005340 | 37.879 | 198 | 115 | 4 | 15 | 205 | 19 | 215 | 6.89e-32 | 115 |
| MS.gene25137.t1 | MTR_1g023770 | 33.010 | 206 | 126 | 4 | 6 | 205 | 10 | 209 | 7.28e-32 | 115 |
| MS.gene25137.t1 | MTR_6g005380 | 37.500 | 192 | 111 | 4 | 11 | 194 | 15 | 205 | 1.68e-28 | 106 |
| MS.gene25137.t1 | MTR_2g030895 | 35.465 | 172 | 104 | 4 | 41 | 205 | 7 | 178 | 6.40e-28 | 104 |
| MS.gene25137.t1 | MTR_8g007300 | 33.708 | 178 | 105 | 4 | 3 | 169 | 5 | 180 | 1.64e-26 | 101 |
| MS.gene25137.t1 | MTR_2g030865 | 35.465 | 172 | 104 | 4 | 41 | 205 | 7 | 178 | 2.56e-26 | 100 |
| MS.gene25137.t1 | MTR_5g036570 | 31.937 | 191 | 125 | 4 | 22 | 207 | 31 | 221 | 9.64e-26 | 99.8 |
| MS.gene25137.t1 | MTR_3g096330 | 34.503 | 171 | 103 | 2 | 8 | 169 | 561 | 731 | 2.00e-25 | 103 |
| MS.gene25137.t1 | MTR_6g005350 | 34.010 | 197 | 123 | 4 | 15 | 205 | 19 | 214 | 2.66e-25 | 98.6 |
| MS.gene25137.t1 | MTR_2g086630 | 38.365 | 159 | 92 | 4 | 52 | 205 | 47 | 204 | 1.73e-23 | 93.6 |
| MS.gene25137.t1 | MTR_2g086620 | 37.736 | 159 | 93 | 4 | 52 | 205 | 47 | 204 | 9.50e-23 | 91.7 |
| MS.gene25137.t1 | MTR_2g031300 | 44.340 | 106 | 55 | 3 | 41 | 142 | 7 | 112 | 7.70e-22 | 87.8 |
| MS.gene25137.t1 | MTR_2g086640 | 36.025 | 161 | 97 | 4 | 52 | 207 | 46 | 205 | 1.23e-20 | 85.9 |
| MS.gene25137.t1 | MTR_2g096110 | 35.915 | 142 | 74 | 3 | 40 | 178 | 2 | 129 | 3.12e-19 | 80.5 |
| MS.gene25137.t1 | MTR_4g052770 | 36.296 | 135 | 84 | 2 | 73 | 205 | 71 | 205 | 9.39e-19 | 80.9 |
| MS.gene25137.t1 | MTR_4g052780 | 36.296 | 135 | 84 | 2 | 73 | 205 | 71 | 205 | 2.07e-18 | 80.1 |
| MS.gene25137.t1 | MTR_7g029500 | 52.830 | 53 | 25 | 0 | 150 | 202 | 38 | 90 | 1.90e-13 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25137.t1 | AT1G72610 | 60.622 | 193 | 74 | 2 | 19 | 209 | 16 | 208 | 2.43e-77 | 231 |
| MS.gene25137.t1 | AT5G20630 | 55.981 | 209 | 90 | 2 | 1 | 207 | 1 | 209 | 4.17e-76 | 228 |
| MS.gene25137.t1 | AT5G39160 | 40.206 | 194 | 106 | 4 | 20 | 206 | 26 | 216 | 4.69e-40 | 136 |
| MS.gene25137.t1 | AT5G39190 | 40.816 | 196 | 106 | 5 | 20 | 206 | 26 | 220 | 8.96e-40 | 135 |
| MS.gene25137.t1 | AT5G39160 | 40.306 | 196 | 107 | 5 | 20 | 206 | 26 | 220 | 3.45e-39 | 134 |
| MS.gene25137.t1 | AT3G04200 | 39.378 | 193 | 109 | 5 | 22 | 206 | 31 | 223 | 4.89e-39 | 134 |
| MS.gene25137.t1 | AT5G26700 | 37.198 | 207 | 126 | 4 | 5 | 208 | 7 | 212 | 5.50e-39 | 134 |
| MS.gene25137.t1 | AT3G05950 | 41.451 | 193 | 103 | 5 | 22 | 205 | 30 | 221 | 7.20e-39 | 134 |
| MS.gene25137.t1 | AT5G39130 | 40.816 | 196 | 106 | 5 | 20 | 206 | 26 | 220 | 7.65e-39 | 133 |
| MS.gene25137.t1 | AT1G09560 | 40.957 | 188 | 107 | 3 | 22 | 205 | 28 | 215 | 4.41e-38 | 131 |
| MS.gene25137.t1 | AT3G62020 | 35.545 | 211 | 126 | 5 | 8 | 208 | 8 | 218 | 4.94e-38 | 131 |
| MS.gene25137.t1 | AT5G39110 | 38.182 | 220 | 121 | 5 | 1 | 205 | 1 | 220 | 5.55e-38 | 131 |
| MS.gene25137.t1 | AT1G18980 | 36.765 | 204 | 123 | 4 | 6 | 205 | 17 | 218 | 5.68e-38 | 131 |
| MS.gene25137.t1 | AT1G02335 | 35.211 | 213 | 130 | 4 | 4 | 208 | 6 | 218 | 1.38e-37 | 130 |
| MS.gene25137.t1 | AT3G05930 | 39.153 | 189 | 110 | 3 | 22 | 205 | 27 | 215 | 2.81e-37 | 129 |
| MS.gene25137.t1 | AT5G39180 | 38.182 | 220 | 120 | 7 | 1 | 205 | 1 | 219 | 1.84e-36 | 127 |
| MS.gene25137.t1 | AT5G39120 | 37.963 | 216 | 122 | 6 | 1 | 205 | 5 | 219 | 3.26e-36 | 127 |
| MS.gene25137.t1 | AT5G39150 | 38.182 | 220 | 120 | 7 | 1 | 205 | 1 | 219 | 6.22e-36 | 126 |
| MS.gene25137.t1 | AT3G62020 | 37.853 | 177 | 105 | 4 | 37 | 208 | 13 | 189 | 9.69e-36 | 124 |
| MS.gene25137.t1 | AT5G39190 | 39.888 | 178 | 99 | 3 | 37 | 206 | 21 | 198 | 4.46e-35 | 123 |
| MS.gene25137.t1 | AT5G39160 | 39.326 | 178 | 100 | 3 | 37 | 206 | 21 | 198 | 1.44e-34 | 122 |
| MS.gene25137.t1 | AT3G04150 | 35.204 | 196 | 119 | 4 | 22 | 209 | 30 | 225 | 1.12e-33 | 120 |
| MS.gene25137.t1 | AT1G18970 | 35.821 | 201 | 123 | 4 | 9 | 205 | 20 | 218 | 2.04e-33 | 119 |
| MS.gene25137.t1 | AT3G10080 | 38.265 | 196 | 103 | 7 | 22 | 205 | 30 | 219 | 2.65e-33 | 119 |
| MS.gene25137.t1 | AT5G38960 | 35.468 | 203 | 114 | 5 | 17 | 206 | 25 | 223 | 2.85e-33 | 119 |
| MS.gene25137.t1 | AT3G04150 | 35.556 | 180 | 109 | 3 | 37 | 209 | 59 | 238 | 6.75e-33 | 119 |
| MS.gene25137.t1 | AT3G10080 | 38.265 | 196 | 103 | 7 | 22 | 205 | 101 | 290 | 1.43e-32 | 119 |
| MS.gene25137.t1 | AT5G38940 | 38.660 | 194 | 111 | 5 | 20 | 205 | 28 | 221 | 4.65e-32 | 116 |
| MS.gene25137.t1 | AT5G38940 | 38.660 | 194 | 111 | 5 | 20 | 205 | 52 | 245 | 6.84e-32 | 116 |
| MS.gene25137.t1 | AT1G74820 | 34.101 | 217 | 129 | 5 | 1 | 205 | 11 | 225 | 9.86e-32 | 115 |
| MS.gene25137.t1 | AT4G14630 | 35.211 | 213 | 120 | 6 | 7 | 205 | 14 | 222 | 3.08e-31 | 114 |
| MS.gene25137.t1 | AT5G38930 | 37.056 | 197 | 116 | 5 | 17 | 205 | 25 | 221 | 2.03e-30 | 112 |
| MS.gene25137.t1 | AT5G38910 | 35.874 | 223 | 121 | 6 | 1 | 205 | 1 | 219 | 2.58e-30 | 111 |
| MS.gene25137.t1 | AT5G61750 | 41.176 | 136 | 78 | 2 | 37 | 170 | 40 | 175 | 8.78e-30 | 110 |
| MS.gene25137.t1 | AT3G04170 | 33.482 | 224 | 124 | 7 | 1 | 205 | 1 | 218 | 2.12e-29 | 109 |
| MS.gene25137.t1 | AT3G04190 | 31.841 | 201 | 129 | 4 | 13 | 205 | 21 | 221 | 3.31e-29 | 108 |
| MS.gene25137.t1 | AT3G04180 | 31.841 | 201 | 129 | 4 | 13 | 205 | 21 | 221 | 2.63e-27 | 103 |
| MS.gene25137.t1 | AT1G10460 | 32.222 | 180 | 93 | 4 | 7 | 183 | 38 | 191 | 1.90e-19 | 83.2 |
| MS.gene25137.t1 | AT5G38910 | 30.000 | 220 | 101 | 6 | 1 | 205 | 1 | 182 | 8.80e-16 | 72.8 |
Find 43 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGAGTTGTTGGTGTTTC+CGG | 0.213827 | 8.4:+72922808 | None:intergenic |
| CAATTCAGAAGGTAAATTAT+TGG | 0.292227 | 8.4:+72922448 | None:intergenic |
| CAATAATTTACCTTCTGAAT+TGG | 0.309787 | 8.4:-72922447 | MS.gene25137:CDS |
| GTTGAAACCGGGCGATCTTA+TGG | 0.323878 | 8.4:-72922588 | MS.gene25137:CDS |
| TGATAGCCTGAAGGAGTATC+TGG | 0.361912 | 8.4:+72922887 | None:intergenic |
| TTTCCAGGCCTTAACGGACT+TGG | 0.385891 | 8.4:-72922752 | MS.gene25137:CDS |
| GTCTTTCATGGTTTCGTAGC+CGG | 0.393248 | 8.4:-72922827 | MS.gene25137:CDS |
| TCTTCACTTGTGCAAGATCA+AGG | 0.425262 | 8.4:+72922408 | None:intergenic |
| AAGAAGCTAAAGACACGTTT+TGG | 0.428168 | 8.4:-72922389 | MS.gene25137:CDS |
| TTATTCTAAGATGTTGAAAC+CGG | 0.428787 | 8.4:-72922600 | MS.gene25137:CDS |
| AAGGCTCCAGATACTCCTTC+AGG | 0.435016 | 8.4:-72922893 | MS.gene25137:CDS |
| TAATTTACCTTCTGAATTGG+TGG | 0.446679 | 8.4:-72922444 | MS.gene25137:CDS |
| AAAATCATTGACAGAAGCTT+GGG | 0.456575 | 8.4:+72922931 | None:intergenic |
| TGCAGCATTGAAAGAGTTGT+TGG | 0.465463 | 8.4:+72922799 | None:intergenic |
| CGGAGATTCCAAGTCCGTTA+AGG | 0.474170 | 8.4:+72922744 | None:intergenic |
| TTTCAAATTAACACTGGTAA+GGG | 0.484943 | 8.4:-72922542 | MS.gene25137:CDS |
| TATCAATAATTCAGTAGCAC+CGG | 0.497389 | 8.4:+72922670 | None:intergenic |
| GAATTATTGATAATAGTTCA+AGG | 0.503777 | 8.4:-72922659 | MS.gene25137:CDS |
| ATTCCAAGTCCGTTAAGGCC+TGG | 0.517650 | 8.4:+72922749 | None:intergenic |
| GTTCAAGGTGAAATAACTGC+TGG | 0.522312 | 8.4:-72922644 | MS.gene25137:CDS |
| AGATCAAGTAATTGAGCACC+AGG | 0.524185 | 8.4:+72922482 | None:intergenic |
| GTCTGATGTGATATTTGCAA+GGG | 0.551461 | 8.4:+72922856 | None:intergenic |
| GCACCGGGATGAGTATGCAT+TGG | 0.553990 | 8.4:+72922686 | None:intergenic |
| ATTGACAGAAGCTTGGGAAG+TGG | 0.571601 | 8.4:+72922937 | None:intergenic |
| ATTCCAATGCATACTCATCC+CGG | 0.573458 | 8.4:-72922689 | MS.gene25137:CDS |
| CGTCTGATGTGATATTTGCA+AGG | 0.576346 | 8.4:+72922855 | None:intergenic |
| GTTTGTGCCACCAATTCAGA+AGG | 0.585911 | 8.4:+72922437 | None:intergenic |
| TGAAAATGCAACATCCCTTG+TGG | 0.593070 | 8.4:+72922560 | None:intergenic |
| ATCAATAATTCAGTAGCACC+GGG | 0.596504 | 8.4:+72922671 | None:intergenic |
| CGCTATGTCTAACCGCGCTG+CGG | 0.604451 | 8.4:+72922724 | None:intergenic |
| ATCTTAGAATAAACCGCGGT+AGG | 0.612903 | 8.4:+72922611 | None:intergenic |
| TATTCTAAGATGTTGAAACC+GGG | 0.635109 | 8.4:-72922599 | MS.gene25137:CDS |
| TGGATTTCTGACACCTACCG+CGG | 0.639675 | 8.4:-72922624 | MS.gene25137:CDS |
| TGGAAAATCAGTGACGAATG+CGG | 0.643979 | 8.4:+72922769 | None:intergenic |
| CGGTTAGACATAGCGGAAGG+TGG | 0.644542 | 8.4:-72922716 | MS.gene25137:CDS |
| CGCAGCGCGGTTAGACATAG+CGG | 0.655117 | 8.4:-72922723 | MS.gene25137:CDS |
| GCGCGGTTAGACATAGCGGA+AGG | 0.662984 | 8.4:-72922719 | MS.gene25137:CDS |
| TCTGATGTGATATTTGCAAG+GGG | 0.669912 | 8.4:+72922857 | None:intergenic |
| GGTTTGCAGTGATAGCCTGA+AGG | 0.681067 | 8.4:+72922878 | None:intergenic |
| AAAATCAGTGACGAATGCGG+AGG | 0.711598 | 8.4:+72922772 | None:intergenic |
| CAACATCTTAGAATAAACCG+CGG | 0.729066 | 8.4:+72922607 | None:intergenic |
| GATCAAGTAATTGAGCACCA+GGG | 0.730668 | 8.4:+72922483 | None:intergenic |
| GACTTGGAATCTCCGCAGCG+CGG | 0.748357 | 8.4:-72922736 | MS.gene25137:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATTATTGATAATAGTTCA+AGG | - | chr8.4:72922700-72922719 | MS.gene25137:CDS | 20.0% |
| !!! | TTTTTGTGTAGCAAATTTAA+AGG | - | chr8.4:72922447-72922466 | MS.gene25137:CDS | 20.0% |
| ! | CAATAATTTACCTTCTGAAT+TGG | - | chr8.4:72922912-72922931 | MS.gene25137:CDS | 25.0% |
| ! | CAATTCAGAAGGTAAATTAT+TGG | + | chr8.4:72922914-72922933 | None:intergenic | 25.0% |
| ! | TTTCAAATTAACACTGGTAA+GGG | - | chr8.4:72922817-72922836 | MS.gene25137:CDS | 25.0% |
| !! | TTGCATTTTCAAATTAACAC+TGG | - | chr8.4:72922811-72922830 | MS.gene25137:CDS | 25.0% |
| !! | TTTTCAAATTAACACTGGTA+AGG | - | chr8.4:72922816-72922835 | MS.gene25137:CDS | 25.0% |
| !!! | TTATTCTAAGATGTTGAAAC+CGG | - | chr8.4:72922759-72922778 | MS.gene25137:CDS | 25.0% |
| AAAAATCATTGACAGAAGCT+TGG | + | chr8.4:72922432-72922451 | None:intergenic | 30.0% | |
| AAAATCATTGACAGAAGCTT+GGG | + | chr8.4:72922431-72922450 | None:intergenic | 30.0% | |
| TAATTTACCTTCTGAATTGG+TGG | - | chr8.4:72922915-72922934 | MS.gene25137:CDS | 30.0% | |
| TATCAATAATTCAGTAGCAC+CGG | + | chr8.4:72922692-72922711 | None:intergenic | 30.0% | |
| ! | ATCTTATGGTTTTTCCACAA+GGG | - | chr8.4:72922785-72922804 | MS.gene25137:CDS | 30.0% |
| !! | TATTCTAAGATGTTGAAACC+GGG | - | chr8.4:72922760-72922779 | MS.gene25137:CDS | 30.0% |
| ATCAATAATTCAGTAGCACC+GGG | + | chr8.4:72922691-72922710 | None:intergenic | 35.0% | |
| CAACATCTTAGAATAAACCG+CGG | + | chr8.4:72922755-72922774 | None:intergenic | 35.0% | |
| GTCTGATGTGATATTTGCAA+GGG | + | chr8.4:72922506-72922525 | None:intergenic | 35.0% | |
| TCTGATGTGATATTTGCAAG+GGG | + | chr8.4:72922505-72922524 | None:intergenic | 35.0% | |
| ! | AAGAAGCTAAAGACACGTTT+TGG | - | chr8.4:72922970-72922989 | MS.gene25137:CDS | 35.0% |
| ! | GATCTTATGGTTTTTCCACA+AGG | - | chr8.4:72922784-72922803 | MS.gene25137:CDS | 35.0% |
| ! | TCAGACGATTTTGTCTTTCA+TGG | - | chr8.4:72922520-72922539 | MS.gene25137:CDS | 35.0% |
| AGATCAAGTAATTGAGCACC+AGG | + | chr8.4:72922880-72922899 | None:intergenic | 40.0% | |
| ATCTTAGAATAAACCGCGGT+AGG | + | chr8.4:72922751-72922770 | None:intergenic | 40.0% | |
| ATTCCAATGCATACTCATCC+CGG | - | chr8.4:72922670-72922689 | MS.gene25137:CDS | 40.0% | |
| CGTCTGATGTGATATTTGCA+AGG | + | chr8.4:72922507-72922526 | None:intergenic | 40.0% | |
| GATCAAGTAATTGAGCACCA+GGG | + | chr8.4:72922879-72922898 | None:intergenic | 40.0% | |
| GTTCAAGGTGAAATAACTGC+TGG | - | chr8.4:72922715-72922734 | MS.gene25137:CDS | 40.0% | |
| TCTTCACTTGTGCAAGATCA+AGG | + | chr8.4:72922954-72922973 | None:intergenic | 40.0% | |
| TGAAAATGCAACATCCCTTG+TGG | + | chr8.4:72922802-72922821 | None:intergenic | 40.0% | |
| TGGAAAATCAGTGACGAATG+CGG | + | chr8.4:72922593-72922612 | None:intergenic | 40.0% | |
| ! | AAGCTAAAGACACGTTTTGG+TGG | - | chr8.4:72922973-72922992 | MS.gene25137:CDS | 40.0% |
| ! | ACTTTTAGTAGTGCTAACCC+TGG | - | chr8.4:72922859-72922878 | MS.gene25137:CDS | 40.0% |
| ! | GAAAGAGTTGTTGGTGTTTC+CGG | + | chr8.4:72922554-72922573 | None:intergenic | 40.0% |
| ! | TGCAGCATTGAAAGAGTTGT+TGG | + | chr8.4:72922563-72922582 | None:intergenic | 40.0% |
| !! | ACTGATTTTCCAGGCCTTAA+CGG | - | chr8.4:72922601-72922620 | MS.gene25137:CDS | 40.0% |
| AAAATCAGTGACGAATGCGG+AGG | + | chr8.4:72922590-72922609 | None:intergenic | 45.0% | |
| ATTGACAGAAGCTTGGGAAG+TGG | + | chr8.4:72922425-72922444 | None:intergenic | 45.0% | |
| GGAAAAACCATAAGATCGCC+CGG | + | chr8.4:72922781-72922800 | None:intergenic | 45.0% | |
| GTCTTTCATGGTTTCGTAGC+CGG | - | chr8.4:72922532-72922551 | MS.gene25137:CDS | 45.0% | |
| GTTTGTGCCACCAATTCAGA+AGG | + | chr8.4:72922925-72922944 | None:intergenic | 45.0% | |
| !! | AAGACACGTTTTGGTGGAAG+AGG | - | chr8.4:72922979-72922998 | MS.gene25137:CDS | 45.0% |
| !! | AGACACGTTTTGGTGGAAGA+GGG | - | chr8.4:72922980-72922999 | MS.gene25137:CDS | 45.0% |
| !! | TGATAGCCTGAAGGAGTATC+TGG | + | chr8.4:72922475-72922494 | None:intergenic | 45.0% |
| !!! | GCATTCGTCACTGATTTTCC+AGG | - | chr8.4:72922592-72922611 | MS.gene25137:CDS | 45.0% |
| AAGGCTCCAGATACTCCTTC+AGG | - | chr8.4:72922466-72922485 | MS.gene25137:CDS | 50.0% | |
| ATTCCAAGTCCGTTAAGGCC+TGG | + | chr8.4:72922613-72922632 | None:intergenic | 50.0% | |
| CGGAGATTCCAAGTCCGTTA+AGG | + | chr8.4:72922618-72922637 | None:intergenic | 50.0% | |
| GGTTTGCAGTGATAGCCTGA+AGG | + | chr8.4:72922484-72922503 | None:intergenic | 50.0% | |
| GTTGAAACCGGGCGATCTTA+TGG | - | chr8.4:72922771-72922790 | MS.gene25137:CDS | 50.0% | |
| TGGATTTCTGACACCTACCG+CGG | - | chr8.4:72922735-72922754 | MS.gene25137:CDS | 50.0% | |
| !! | TTTCCAGGCCTTAACGGACT+TGG | - | chr8.4:72922607-72922626 | MS.gene25137:CDS | 50.0% |
| GCACCGGGATGAGTATGCAT+TGG | + | chr8.4:72922676-72922695 | None:intergenic | 55.0% | |
| ! | CGGTTAGACATAGCGGAAGG+TGG | - | chr8.4:72922643-72922662 | MS.gene25137:CDS | 55.0% |
| CGCAGCGCGGTTAGACATAG+CGG | - | chr8.4:72922636-72922655 | MS.gene25137:CDS | 60.0% | |
| CGCTATGTCTAACCGCGCTG+CGG | + | chr8.4:72922638-72922657 | None:intergenic | 60.0% | |
| GACTTGGAATCTCCGCAGCG+CGG | - | chr8.4:72922623-72922642 | MS.gene25137:CDS | 60.0% | |
| ! | GCGCGGTTAGACATAGCGGA+AGG | - | chr8.4:72922640-72922659 | MS.gene25137:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 72922376 | 72923005 | 72922376 | ID=MS.gene25137 |
| chr8.4 | mRNA | 72922376 | 72923005 | 72922376 | ID=MS.gene25137.t1;Parent=MS.gene25137 |
| chr8.4 | exon | 72922376 | 72923005 | 72922376 | ID=MS.gene25137.t1.exon1;Parent=MS.gene25137.t1 |
| chr8.4 | CDS | 72922376 | 72923005 | 72922376 | ID=cds.MS.gene25137.t1;Parent=MS.gene25137.t1 |
| Gene Sequence |
| Protein sequence |
