Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25218.t1 | XP_013450245.1 | 96 | 401 | 16 | 0 | 1 | 401 | 1 | 401 | 8.80E-221 | 776.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25218.t1 | Q6RFH5 | 37.3 | 185 | 110 | 5 | 180 | 361 | 149 | 330 | 1.4e-25 | 119.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25218.t1 | A0A072U548 | 96.0 | 401 | 16 | 0 | 1 | 401 | 1 | 401 | 6.3e-221 | 776.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene25218 | 0.805493 | 1.30E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene25218 | 0.802007 | 6.88E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene25218 | 0.811447 | 6.97E-51 | -1.69E-46 |
| MS.gene053080 | MS.gene25218 | 0.807787 | 4.26E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene25218 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene25218 | 0.805456 | 1.32E-49 | -1.69E-46 |
| MS.gene05978 | MS.gene25218 | 0.808945 | 2.41E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene25218 | 0.801298 | 9.62E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene25218 | 0.800668 | 1.29E-48 | -1.69E-46 |
| MS.gene060429 | MS.gene25218 | 0.801652 | 8.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25218.t1 | MTR_7g106650 | 90.069 | 433 | 39 | 1 | 1 | 433 | 1 | 429 | 0.0 | 801 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25218.t1 | AT1G29320 | 56.923 | 390 | 165 | 2 | 1 | 387 | 1 | 390 | 6.66e-153 | 443 |
| MS.gene25218.t1 | AT1G29320 | 57.143 | 392 | 162 | 4 | 1 | 387 | 1 | 391 | 6.70e-152 | 441 |
Find 108 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGAGAAGTTTGCTTTATT+TGG | 0.096155 | 7.3:-7430251 | MS.gene25218:CDS |
| AAGGCGTTGAAGTTAATATT+TGG | 0.160856 | 7.3:-7429530 | MS.gene25218:CDS |
| TCTGAAGAGAATAATGTTAT+TGG | 0.190952 | 7.3:-7432604 | MS.gene25218:CDS |
| CTAACCTTCAACAATGAAAT+TGG | 0.239759 | 7.3:+7425853 | None:intergenic |
| CTCATGTTCATCATTTCCTT+TGG | 0.278219 | 7.3:+7425220 | None:intergenic |
| TGGATTCAAACTCAAGATTC+TGG | 0.297251 | 7.3:+7432563 | None:intergenic |
| CATACATCCCAATGTTAGTT+TGG | 0.310120 | 7.3:+7425065 | None:intergenic |
| TCCTCGGCCAGTGCTTTAAT+TGG | 0.319494 | 7.3:+7428166 | None:intergenic |
| GGATGGCTATTTACGACTTT+GGG | 0.333336 | 7.3:-7427103 | MS.gene25218:CDS |
| TGGTAGCGAATCCGCTCCTT+TGG | 0.333516 | 7.3:+7425394 | None:intergenic |
| TTCATAGTTTCTTCCTTGCT+TGG | 0.344943 | 7.3:+7425374 | None:intergenic |
| ACCTGGAATGTTTGTAATAG+TGG | 0.345723 | 7.3:-7430302 | MS.gene25218:CDS |
| AGGCGTTGAAGTTAATATTT+GGG | 0.363980 | 7.3:-7429529 | MS.gene25218:CDS |
| GAAGATCCTGTCGCTGAATC+AGG | 0.366839 | 7.3:+7430344 | None:intergenic |
| GATGGCAACACAATCTATCT+AGG | 0.371926 | 7.3:-7428139 | MS.gene25218:CDS |
| TTGATAACTGCACGAAGATC+TGG | 0.374516 | 7.3:-7429503 | MS.gene25218:CDS |
| GGGTTCATGGCTCACAGATT+TGG | 0.379867 | 7.3:+7429476 | None:intergenic |
| CAATGTTAGTTTGGAATCCT+TGG | 0.381539 | 7.3:+7425074 | None:intergenic |
| GATTCCAATTTCATTGTTGA+AGG | 0.393194 | 7.3:-7425857 | MS.gene25218:intron |
| TGGATGGCTATTTACGACTT+TGG | 0.400867 | 7.3:-7427104 | MS.gene25218:CDS |
| CTAAATCACAACCCTTCATC+TGG | 0.401575 | 7.3:+7425125 | None:intergenic |
| AATGAGTGAAACAGCGTTAA+CGG | 0.407170 | 7.3:+7433196 | None:intergenic |
| AATAAAGAAAATATGATAGA+TGG | 0.407882 | 7.3:-7425290 | MS.gene25218:intron |
| GACCATCGAAAATTTGTTGC+TGG | 0.414825 | 7.3:-7429263 | MS.gene25218:CDS |
| GGTAGCGAATCCGCTCCTTT+GGG | 0.415243 | 7.3:+7425395 | None:intergenic |
| ACCACTATTACAAACATTCC+AGG | 0.418193 | 7.3:+7430301 | None:intergenic |
| TAAAAGTACCTGATGGTTAC+TGG | 0.430696 | 7.3:+7429238 | None:intergenic |
| TTATAATTTACTTACATGTA+CGG | 0.443505 | 7.3:-7430402 | MS.gene25218:CDS |
| CATGCAGAATAGGCTGCTTA+AGG | 0.458100 | 7.3:+7425888 | None:intergenic |
| GAAGCTACCACAGGTAGCTC+AGG | 0.460028 | 7.3:+7427486 | None:intergenic |
| GAAGTTCCTGATTCAGCGAC+AGG | 0.460401 | 7.3:-7430350 | MS.gene25218:CDS |
| GTTAACGGACTTAGTTGAAT+CGG | 0.462040 | 7.3:+7433211 | None:intergenic |
| TATAATTTACTTACATGTAC+GGG | 0.462972 | 7.3:-7430401 | MS.gene25218:CDS |
| CGAAAATCAATAGAGAGAAC+AGG | 0.464845 | 7.3:+7428196 | None:intergenic |
| AAATATGAAATTCCAGATGA+AGG | 0.466225 | 7.3:-7425137 | MS.gene25218:CDS |
| ACCCCACCTATCTACTACCT+TGG | 0.469187 | 7.3:+7433238 | None:intergenic |
| ATGAAGGGTTGTGATTTAGC+TGG | 0.470545 | 7.3:-7425121 | MS.gene25218:CDS |
| TCTGAGCAGATATGTCATAA+AGG | 0.472800 | 7.3:+7428224 | None:intergenic |
| GAGAAGTTTGCTTTATTTGG+AGG | 0.475298 | 7.3:-7430248 | MS.gene25218:CDS |
| TACTTACATGTACGGGCAAA+GGG | 0.477939 | 7.3:-7430394 | MS.gene25218:CDS |
| TTAACGGACTTAGTTGAATC+GGG | 0.480921 | 7.3:+7433212 | None:intergenic |
| GCTAGAGAAACTCCAGTGAC+AGG | 0.483353 | 7.3:+7432661 | None:intergenic |
| GCAAAGGGAATGCAAGCATA+AGG | 0.487266 | 7.3:-7430379 | MS.gene25218:CDS |
| CCCCACCTATCTACTACCTT+GGG | 0.490032 | 7.3:+7433239 | None:intergenic |
| GCTCTAAGTGGAGGACAACC+AGG | 0.491508 | 7.3:+7433391 | None:intergenic |
| AACACAATCTATCTAGGGAA+TGG | 0.494679 | 7.3:-7428133 | MS.gene25218:CDS |
| TTACTTACATGTACGGGCAA+AGG | 0.498260 | 7.3:-7430395 | MS.gene25218:CDS |
| CAAGGATTCCAAACTAACAT+TGG | 0.501620 | 7.3:-7425073 | MS.gene25218:CDS |
| TTAAATACAGGTAAAATGCT+AGG | 0.502823 | 7.3:-7427558 | MS.gene25218:intron |
| ACCAATTAAAGCACTGGCCG+AGG | 0.503185 | 7.3:-7428167 | MS.gene25218:CDS |
| GAAGCATCAGATCCATTGTC+AGG | 0.503948 | 7.3:-7427512 | MS.gene25218:CDS |
| AGACTGAAGATCTAAATCAT+TGG | 0.509744 | 7.3:+7432624 | None:intergenic |
| AAGGATTCCAAACTAACATT+GGG | 0.510383 | 7.3:-7425072 | MS.gene25218:CDS |
| ATCTATCTAGGGAATGGGTC+TGG | 0.511093 | 7.3:-7428127 | MS.gene25218:CDS |
| TCGCTGAATCAGGAACTTCA+AGG | 0.514181 | 7.3:+7430354 | None:intergenic |
| AGAAGTTTGCTTTATTTGGA+GGG | 0.524343 | 7.3:-7430247 | MS.gene25218:intron |
| GCTTTAACCTTCGACACACT+TGG | 0.529551 | 7.3:-7433370 | MS.gene25218:CDS |
| ACACAATCTATCTAGGGAAT+GGG | 0.530689 | 7.3:-7428132 | MS.gene25218:CDS |
| AATATGAAATTCCAGATGAA+GGG | 0.531290 | 7.3:-7425136 | MS.gene25218:CDS |
| TGCTGGCACCAGTAACCATC+AGG | 0.531889 | 7.3:-7429246 | MS.gene25218:intron |
| AGGGAATGGGTCTGGTGACA+TGG | 0.542984 | 7.3:-7428119 | MS.gene25218:CDS |
| TAGCTCAGGGTGCCTGACAA+TGG | 0.550961 | 7.3:+7427500 | None:intergenic |
| AGCGGATTCGCTACCAAGCA+AGG | 0.551373 | 7.3:-7425387 | MS.gene25218:CDS |
| GAGGTCCCAAGGTAGTAGAT+AGG | 0.555775 | 7.3:-7433244 | MS.gene25218:CDS |
| TTGATATATTCACACCTACC+TGG | 0.557164 | 7.3:-7429316 | MS.gene25218:CDS |
| GACACACTTGGTCTTATCAA+AGG | 0.560611 | 7.3:-7433358 | MS.gene25218:intron |
| TCCCAAGGTAGTAGATAGGT+GGG | 0.565727 | 7.3:-7433240 | MS.gene25218:CDS |
| GCACTGGCCGAGGATATAGA+TGG | 0.567128 | 7.3:-7428157 | MS.gene25218:CDS |
| GCTACCACTGTAGAGTGCCC+TGG | 0.568003 | 7.3:-7433409 | MS.gene25218:CDS |
| GATAAGACCAAGTGTGTCGA+AGG | 0.570294 | 7.3:+7433363 | None:intergenic |
| GCTTCTGTTGATATACGTAC+AGG | 0.571055 | 7.3:-7428097 | MS.gene25218:intron |
| AGGTAGGTGTGAATATATCA+AGG | 0.573013 | 7.3:+7429318 | None:intergenic |
| GGTATTGCAGTTATTAGCTG+TGG | 0.574259 | 7.3:-7432823 | MS.gene25218:intron |
| TATGAAAGAGATCACGGATG+GGG | 0.574536 | 7.3:-7425357 | MS.gene25218:CDS |
| AAGAAGAAAACGTACCGGGT+TGG | 0.576937 | 7.3:+7433163 | None:intergenic |
| TGAGACACCAATTAAAGCAC+TGG | 0.577646 | 7.3:-7428173 | MS.gene25218:CDS |
| CTCAGATACACCCAAAGGAG+CGG | 0.581212 | 7.3:-7425405 | MS.gene25218:intron |
| AGAATCTTGAGTTTGAATCC+AGG | 0.582034 | 7.3:-7432561 | MS.gene25218:intron |
| ACTATGAAAGAGATCACGGA+TGG | 0.583209 | 7.3:-7425359 | MS.gene25218:CDS |
| CTATGAAAGAGATCACGGAT+GGG | 0.584420 | 7.3:-7425358 | MS.gene25218:CDS |
| TGTGTTGCCATCTATATCCT+CGG | 0.585169 | 7.3:+7428150 | None:intergenic |
| GTCCCAAGGTAGTAGATAGG+TGG | 0.586983 | 7.3:-7433241 | MS.gene25218:CDS |
| ATGGCAACACAATCTATCTA+GGG | 0.590346 | 7.3:-7428138 | MS.gene25218:CDS |
| CGCTGAATCAGGAACTTCAA+GGG | 0.591335 | 7.3:+7430355 | None:intergenic |
| AAGACGGCGTCAAGAGACAA+AGG | 0.601694 | 7.3:-7425197 | MS.gene25218:CDS |
| AAATGATGAACATGAGAAGA+CGG | 0.601744 | 7.3:-7425213 | MS.gene25218:CDS |
| AGAAACTATGAAAGAGATCA+CGG | 0.602289 | 7.3:-7425363 | MS.gene25218:CDS |
| CTACCTGTGGTAGCTTCATG+TGG | 0.603083 | 7.3:-7427480 | MS.gene25218:intron |
| CTAGAGAAACTCCAGTGACA+GGG | 0.603489 | 7.3:+7432662 | None:intergenic |
| CACTCTACAGTGGTAGCGCG+AGG | 0.605120 | 7.3:+7433415 | None:intergenic |
| ATATGAAACAGATGTGAACC+AGG | 0.611012 | 7.3:+7429298 | None:intergenic |
| TCGAAGGTTAAAGCTCTAAG+TGG | 0.615123 | 7.3:+7433379 | None:intergenic |
| CATTGATCGCAAATCCAACC+CGG | 0.624320 | 7.3:-7433177 | MS.gene25218:intron |
| GAAGAACACACAAAATCCAA+AGG | 0.629527 | 7.3:-7425236 | MS.gene25218:CDS |
| CAGGCACCCTGAGCTACCTG+TGG | 0.634317 | 7.3:-7427493 | MS.gene25218:CDS |
| AAGCTACCACAGGTAGCTCA+GGG | 0.640900 | 7.3:+7427487 | None:intergenic |
| AAGGTTAAAGCTCTAAGTGG+AGG | 0.655539 | 7.3:+7433382 | None:intergenic |
| GAAACAGATGTGAACCAGGT+AGG | 0.656614 | 7.3:+7429302 | None:intergenic |
| TGGTAACGTCTTGTGTTGCA+AGG | 0.665311 | 7.3:-7430282 | MS.gene25218:CDS |
| ATTTACTCAGATACACCCAA+AGG | 0.670340 | 7.3:-7425410 | MS.gene25218:intron |
| TCACCACATGAAGCTACCAC+AGG | 0.684230 | 7.3:+7427477 | None:intergenic |
| CCCAAGGTAGTAGATAGGTG+GGG | 0.685917 | 7.3:-7433239 | MS.gene25218:CDS |
| ACAACCAGGGCACTCTACAG+TGG | 0.689442 | 7.3:+7433405 | None:intergenic |
| GACTTTGGGATACAAAGACA+AGG | 0.696742 | 7.3:-7427089 | MS.gene25218:CDS |
| CTCTAAGTGGAGGACAACCA+GGG | 0.698739 | 7.3:+7433392 | None:intergenic |
| ACTATAGGATATATACACCA+AGG | 0.701279 | 7.3:-7425091 | MS.gene25218:CDS |
| TTTATGACATATCTGCTCAG+AGG | 0.715403 | 7.3:-7428222 | MS.gene25218:CDS |
| TAACGTCTTGTGTTGCAAGG+TGG | 0.722177 | 7.3:-7430279 | MS.gene25218:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 7425066 | 7433440 | 7425066 | ID=MS.gene25218 |
| chr7.3 | mRNA | 7425066 | 7433440 | 7425066 | ID=MS.gene25218.t1;Parent=MS.gene25218 |
| chr7.3 | exon | 7433359 | 7433440 | 7433359 | ID=MS.gene25218.t1.exon1;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7433359 | 7433440 | 7433359 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7433178 | 7433290 | 7433178 | ID=MS.gene25218.t1.exon2;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7433178 | 7433290 | 7433178 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7432806 | 7432835 | 7432806 | ID=MS.gene25218.t1.exon3;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7432806 | 7432835 | 7432806 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7432562 | 7432698 | 7432562 | ID=MS.gene25218.t1.exon4;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7432562 | 7432698 | 7432562 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7430248 | 7430427 | 7430248 | ID=MS.gene25218.t1.exon5;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7430248 | 7430427 | 7430248 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7429491 | 7429554 | 7429491 | ID=MS.gene25218.t1.exon6;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7429491 | 7429554 | 7429491 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7429247 | 7429354 | 7429247 | ID=MS.gene25218.t1.exon7;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7429247 | 7429354 | 7429247 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7428098 | 7428251 | 7428098 | ID=MS.gene25218.t1.exon8;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7428098 | 7428251 | 7428098 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7427481 | 7427570 | 7427481 | ID=MS.gene25218.t1.exon9;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7427481 | 7427570 | 7427481 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7427074 | 7427129 | 7427074 | ID=MS.gene25218.t1.exon10;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7427074 | 7427129 | 7427074 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7425858 | 7425915 | 7425858 | ID=MS.gene25218.t1.exon11;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7425858 | 7425915 | 7425858 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7425291 | 7425422 | 7425291 | ID=MS.gene25218.t1.exon12;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7425291 | 7425422 | 7425291 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| chr7.3 | exon | 7425066 | 7425259 | 7425066 | ID=MS.gene25218.t1.exon13;Parent=MS.gene25218.t1 |
| chr7.3 | CDS | 7425066 | 7425259 | 7425066 | ID=cds.MS.gene25218.t1;Parent=MS.gene25218.t1 |
| Gene Sequence |
| Protein sequence |
