AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25300.t1 MTR_4g009040 93.538 325 20 1 1 324 1 325 0.0 618
MS.gene25300.t1 MTR_4g009040 92.401 329 20 2 1 324 1 329 0.0 612
MS.gene25300.t1 MTR_4g009690 92.615 325 24 0 1 325 1 325 0.0 598
MS.gene25300.t1 MTR_4g009690 92.331 326 24 1 1 325 1 326 0.0 594
MS.gene25300.t1 MTR_4g006940 47.222 324 158 5 4 322 11 326 8.28e-89 269
MS.gene25300.t1 MTR_2g104960 47.302 315 156 4 13 322 20 329 3.42e-86 263
MS.gene25300.t1 MTR_5g072620 42.188 320 176 4 10 322 2 319 3.03e-82 252
MS.gene25300.t1 MTR_4g006940 50.181 277 129 4 4 279 11 279 1.30e-81 249
MS.gene25300.t1 MTR_4g006940 50.181 277 129 4 4 279 11 279 1.30e-81 249
MS.gene25300.t1 MTR_3g005170 43.770 313 167 6 13 318 10 320 4.69e-80 246
MS.gene25300.t1 MTR_3g005210 43.849 317 169 6 9 318 2 316 3.28e-79 244
MS.gene25300.t1 MTR_4g077100 42.722 316 170 7 11 318 9 321 3.49e-77 239
MS.gene25300.t1 MTR_2g101330 41.745 321 176 5 12 324 4 321 5.77e-77 239
MS.gene25300.t1 MTR_8g062440 44.062 320 167 8 11 322 6 321 2.08e-76 237
MS.gene25300.t1 MTR_4g077130 42.320 319 173 7 8 318 4 319 1.22e-75 235
MS.gene25300.t1 MTR_8g062440 44.237 321 166 9 11 322 6 322 1.35e-75 235
MS.gene25300.t1 MTR_8g062110 43.533 317 167 8 10 318 6 318 8.68e-74 230
MS.gene25300.t1 MTR_8g062110 43.533 317 167 8 10 318 15 327 1.20e-73 230
MS.gene25300.t1 MTR_3g005170 45.185 270 142 5 13 278 10 277 2.83e-73 228
MS.gene25300.t1 MTR_3g031650 38.994 318 189 3 10 322 2 319 5.01e-73 228
MS.gene25300.t1 MTR_2g028620 40.244 328 179 8 8 323 5 327 1.70e-71 225
MS.gene25300.t1 MTR_4g077100 44.118 272 146 5 11 278 9 278 3.61e-71 222
MS.gene25300.t1 MTR_8g062440 46.442 267 136 6 11 273 6 269 9.30e-69 218
MS.gene25300.t1 MTR_1g022440 38.272 324 186 10 10 322 5 325 7.05e-68 216
MS.gene25300.t1 MTR_3g005170 39.936 313 148 6 13 318 10 289 1.17e-67 214
MS.gene25300.t1 MTR_8g062110 42.857 287 150 7 10 292 6 282 8.38e-67 211
MS.gene25300.t1 MTR_8g062110 44.280 271 144 6 10 276 6 273 9.99e-67 211
MS.gene25300.t1 MTR_3g031650 39.552 268 160 2 10 275 2 269 4.43e-66 209
MS.gene25300.t1 MTR_2g028620 43.636 275 145 6 8 275 5 276 5.15e-66 210
MS.gene25300.t1 MTR_5g056600 36.250 320 192 6 12 322 3 319 1.20e-65 209
MS.gene25300.t1 MTR_1g022445 37.695 321 186 9 13 322 8 325 1.64e-65 209
MS.gene25300.t1 MTR_4g092080 38.369 331 179 10 11 321 12 337 2.18e-57 189
MS.gene25300.t1 MTR_7g074880 36.533 323 190 8 12 322 7 326 1.25e-53 179
MS.gene25300.t1 MTR_7g074730 36.196 326 189 10 12 322 7 328 5.38e-52 174
MS.gene25300.t1 MTR_7g074820 36.810 326 188 9 12 322 7 329 1.32e-51 173
MS.gene25300.t1 MTR_4g081440 33.811 349 201 11 1 324 8 351 3.26e-51 173
MS.gene25300.t1 MTR_4g081440 33.429 347 206 10 1 324 12 356 4.67e-50 170
MS.gene25300.t1 MTR_7g074850 34.049 326 197 9 12 322 7 329 2.69e-47 162
MS.gene25300.t1 MTR_4g023730 35.736 333 190 12 8 322 3 329 2.71e-45 157
MS.gene25300.t1 MTR_7g074710 33.951 324 197 11 12 322 7 326 1.70e-44 155
MS.gene25300.t1 MTR_8g062710 75.532 94 13 1 12 105 19 102 6.72e-41 139
MS.gene25300.t1 MTR_8g062110 38.222 225 129 6 101 318 11 232 6.70e-39 137
MS.gene25300.t1 MTR_7g074870 34.875 281 170 8 52 322 11 288 6.88e-37 134
MS.gene25300.t1 MTR_0164s0070 53.543 127 57 2 12 136 4 130 1.27e-36 131
MS.gene25300.t1 MTR_8g062708 85.294 68 10 0 213 280 1 68 4.78e-35 124
MS.gene25300.t1 MTR_5g029990 31.596 307 171 9 13 302 12 296 2.64e-34 127
MS.gene25300.t1 MTR_8g062110 37.989 179 106 4 101 276 11 187 3.08e-32 119
MS.gene25300.t1 MTR_6g406250 31.298 262 172 4 13 268 55 314 4.90e-31 120
MS.gene25300.t1 MTR_7g117190 29.358 327 200 12 12 311 6 328 4.39e-26 106
MS.gene25300.t1 MTR_2g013230 29.275 345 199 14 12 322 7 340 6.29e-26 105
MS.gene25300.t1 MTR_7g117190 30.690 290 171 11 12 275 6 291 1.07e-25 104
MS.gene25300.t1 MTR_6g406250 31.553 206 137 2 13 214 55 260 1.24e-25 103
MS.gene25300.t1 MTR_2g013250 28.280 343 201 12 12 320 6 337 4.60e-24 100
MS.gene25300.t1 MTR_0685s0030 33.962 159 98 2 165 318 2 158 2.14e-22 92.0
MS.gene25300.t1 MTR_2g013260 27.616 344 202 13 12 320 7 338 1.09e-20 91.7
MS.gene25300.t1 MTR_2g013260 35.359 181 98 7 12 179 7 181 2.60e-19 87.0
MS.gene25300.t1 MTR_0685s0030 33.333 114 76 0 165 278 2 115 1.03e-16 75.5
MS.gene25300.t1 MTR_3g011130 37.607 117 68 4 104 216 30 145 3.52e-15 72.0
MS.gene25300.t1 MTR_3g101500 30.233 258 143 12 11 256 2 234 5.92e-13 68.9
MS.gene25300.t1 MTR_3g101500 30.233 258 143 12 11 256 2 234 7.80e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25300.t1 AT2G33590 61.342 313 119 1 12 322 8 320 2.30e-138 395
MS.gene25300.t1 AT2G33600 60.064 313 123 1 12 322 8 320 6.53e-129 371
MS.gene25300.t1 AT1G76470 54.717 318 138 3 11 322 5 322 8.56e-111 325
MS.gene25300.t1 AT1G09510 47.205 322 161 6 8 322 3 322 1.18e-86 263
MS.gene25300.t1 AT1G51410 46.106 321 162 7 11 323 7 324 2.09e-86 263
MS.gene25300.t1 AT1G80820 45.141 319 165 4 9 322 4 317 1.71e-84 258
MS.gene25300.t1 AT5G19440 44.848 330 170 7 1 323 1 325 3.88e-83 254
MS.gene25300.t1 AT1G15950 44.654 318 166 4 10 322 10 322 4.48e-80 247
MS.gene25300.t1 AT1G15950 44.654 318 166 4 10 322 10 322 4.48e-80 247
MS.gene25300.t1 AT1G09490 42.138 318 177 5 11 322 6 322 2.81e-78 242
MS.gene25300.t1 AT1G80820 48.529 272 134 3 9 279 4 270 2.29e-77 238
MS.gene25300.t1 AT1G09480 43.082 318 174 5 11 322 6 322 6.29e-77 238
MS.gene25300.t1 AT1G15950 44.481 308 161 4 10 312 10 312 1.34e-76 238
MS.gene25300.t1 AT5G58490 41.905 315 178 3 13 322 9 323 2.27e-76 237
MS.gene25300.t1 AT1G09480 43.082 318 174 5 11 322 53 369 2.45e-76 238
MS.gene25300.t1 AT1G15950 47.826 276 138 3 10 284 10 280 3.76e-76 235
MS.gene25300.t1 AT1G66800 44.828 319 163 7 11 322 6 318 4.44e-76 236
MS.gene25300.t1 AT1G15950 48.352 273 135 3 10 281 10 277 8.71e-76 234
MS.gene25300.t1 AT1G15950 48.339 271 134 3 10 279 10 275 6.70e-75 232
MS.gene25300.t1 AT4G35420 41.176 323 174 7 12 324 7 323 2.64e-74 232
MS.gene25300.t1 AT1G09500 41.796 323 180 5 8 322 3 325 4.03e-74 231
MS.gene25300.t1 AT2G02400 38.801 317 183 6 13 322 6 318 2.93e-73 229
MS.gene25300.t1 AT1G09500 44.815 270 146 3 8 274 3 272 3.80e-71 222
MS.gene25300.t1 AT5G42800 39.144 327 185 9 7 322 2 325 2.60e-66 213
MS.gene25300.t1 AT1G09490 39.643 280 163 4 48 322 13 291 2.42e-61 197
MS.gene25300.t1 AT1G09500 40.071 282 162 4 48 322 10 291 4.41e-57 186
MS.gene25300.t1 AT1G25460 34.603 315 198 5 15 322 6 319 1.09e-53 179
MS.gene25300.t1 AT1G68540 33.544 316 202 5 15 322 6 321 1.32e-52 176
MS.gene25300.t1 AT2G45400 36.254 331 184 10 13 322 40 364 3.47e-51 173
MS.gene25300.t1 AT1G61720 34.706 340 189 11 10 325 10 340 6.00e-48 164
MS.gene25300.t1 AT4G27250 33.435 329 196 9 1 311 1 324 1.90e-46 160
MS.gene25300.t1 AT2G45400 36.879 282 159 7 13 279 40 317 1.39e-45 157
MS.gene25300.t1 AT4G27250 33.432 338 194 10 1 311 1 334 1.62e-45 158
MS.gene25300.t1 AT1G15950 40.179 224 129 2 104 322 1 224 9.62e-43 148
MS.gene25300.t1 AT2G23910 30.909 275 173 6 14 281 11 275 2.27e-40 143
MS.gene25300.t1 AT2G23910 31.481 270 165 6 14 273 11 270 2.44e-40 142
MS.gene25300.t1 AT2G23910 31.481 270 165 6 14 273 11 270 3.99e-40 143
MS.gene25300.t1 AT2G45400 33.651 315 182 11 27 322 1 307 1.49e-39 141
MS.gene25300.t1 AT4G30470 33.333 270 159 7 14 273 11 269 1.29e-37 136
MS.gene25300.t1 AT2G45400 39.726 219 115 6 13 218 40 254 1.74e-35 129
MS.gene25300.t1 AT1G68540 31.884 207 136 3 121 322 8 214 2.90e-30 114
MS.gene25300.t1 AT2G23910 30.472 233 143 5 50 273 14 236 4.70e-30 115
MS.gene25300.t1 AT5G14700 32.601 273 166 8 9 268 51 318 1.34e-28 114
MS.gene25300.t1 AT5G14700 33.333 216 132 5 9 214 51 264 5.83e-24 99.8
MS.gene25300.t1 AT4G33360 26.786 280 174 12 10 278 12 271 4.64e-15 75.1
MS.gene25300.t1 AT4G33360 26.786 280 174 12 10 278 12 271 8.06e-15 74.7

Find 67 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTATCTTTCGGTAAATT+AGG 0.089094 8.2:+38260848 None:intergenic
CATTTGTCCTACACATGTTT+TGG 0.214166 8.2:-38260518 MS.gene25300:CDS
CTTTATCTTTCGGTAAATTA+GGG 0.276712 8.2:+38260849 None:intergenic
CGTAGTTGATGCAATACTTT+TGG 0.281567 8.2:-38260032 MS.gene25300:CDS
TTTAACAAGTGTTCATATTT+CGG 0.294879 8.2:+38261254 None:intergenic
AATAAGAAGGTGTGTGTAAC+TGG 0.296410 8.2:-38262649 MS.gene25300:CDS
AAAGTTTAGGTTGGAAATTC+AGG 0.321089 8.2:-38259329 MS.gene25300:CDS
CTGAATTTCGCGAAAAGAAC+TGG 0.329580 8.2:-38260556 MS.gene25300:CDS
TTCGTAGGGTACTTGTAGTT+TGG 0.336746 8.2:+38259917 None:intergenic
ATATGAACACTTGTTAAAAC+TGG 0.343713 8.2:-38261249 MS.gene25300:CDS
CACTATCATCATACAAGATT+TGG 0.347439 8.2:-38259963 MS.gene25300:CDS
CTGTTCTTCTGCTTCTGTCT+TGG 0.348831 8.2:+38260582 None:intergenic
ACTCTTCAAAGCAGATATCT+TGG 0.353367 8.2:-38261204 MS.gene25300:CDS
TCAGAGAAACTGCAAAGTTT+AGG 0.360483 8.2:-38259342 MS.gene25300:CDS
TTTGTGGTTGACTGTAAAAT+TGG 0.360540 8.2:+38260493 None:intergenic
TCATCAATCACTTTATCTTT+CGG 0.362730 8.2:+38260839 None:intergenic
AGAAATGTTACCTCAGGATT+AGG 0.364457 8.2:+38261101 None:intergenic
TGATTCTGTTGAGAGCTATA+AGG 0.391851 8.2:-38259286 MS.gene25300:CDS
TCCATGGACAAAGTAACCTT+TGG 0.412376 8.2:+38262582 None:intergenic
GAAAATGAAGCTTACTTCGT+AGG 0.425779 8.2:+38259902 None:intergenic
CAAGACAGAAGCAGAAGAAC+AGG 0.437827 8.2:-38260581 MS.gene25300:CDS
CAAAACATGTGTAGGACAAA+TGG 0.439225 8.2:+38260519 None:intergenic
AGTCAAGCTACTTGTATTTG+TGG 0.440700 8.2:+38260477 None:intergenic
TATCTGCTTTGAAGAGTGTA+AGG 0.460836 8.2:+38261210 None:intergenic
CATGGAACTGTTAGAGAGCC+TGG 0.466759 8.2:-38262565 MS.gene25300:intron
TCGGTTTACTCTGCAATTGT+TGG 0.471908 8.2:-38261173 MS.gene25300:CDS
TCCAAAGGTTACTTTGTCCA+TGG 0.474794 8.2:-38262583 MS.gene25300:CDS
CATTCGAGAATAAGTGTGCT+TGG 0.475236 8.2:-38260072 MS.gene25300:CDS
AGATATCTTGGATTATGAAT+CGG 0.476489 8.2:-38261192 MS.gene25300:CDS
GGTACAACTGTTGAAGGTAC+AGG 0.487565 8.2:+38261122 None:intergenic
AGAAACTGCAAAGTTTAGGT+TGG 0.491298 8.2:-38259338 MS.gene25300:CDS
AAGTGATTGATGAGTCTTGT+TGG 0.503448 8.2:-38260829 MS.gene25300:CDS
GGATTAGGTACAACTGTTGA+AGG 0.511645 8.2:+38261116 None:intergenic
CAGGTGGGTTTGTAGCATCA+TGG 0.513595 8.2:-38262624 MS.gene25300:CDS
TTCCACATTAGCTTTGAGAC+AGG 0.513623 8.2:+38260913 None:intergenic
TTGGAAATTCAGGCCATTGG+AGG 0.514395 8.2:-38259319 MS.gene25300:CDS
AAAATGAAGCTTACTTCGTA+GGG 0.514933 8.2:+38259903 None:intergenic
TATCATCATACAAGATTTGG+TGG 0.521319 8.2:-38259960 MS.gene25300:CDS
AGGTTGGAAATTCAGGCCAT+TGG 0.521698 8.2:-38259322 MS.gene25300:CDS
GTTAAGCTTCTTCTTTCCAA+AGG 0.521805 8.2:-38262598 MS.gene25300:CDS
GTTGAGAGCTATAAGGAGGC+TGG 0.522498 8.2:-38259279 MS.gene25300:CDS
TTAGCAGTTCCCTTCACAGC+AGG 0.530766 8.2:+38260947 None:intergenic
AGGAGATAATGGAAACAAGT+GGG 0.540632 8.2:-38262684 None:intergenic
TCAATGAGTGTTTCCTCCAA+TGG 0.543548 8.2:+38259306 None:intergenic
AGACTACTGCAAGAATACTA+AGG 0.551184 8.2:-38260798 MS.gene25300:intron
CAGGCTCTCTAACAGTTCCA+TGG 0.553939 8.2:+38262566 None:intergenic
AGTGATTGAGCCTGCTGTGA+AGG 0.558611 8.2:-38260957 MS.gene25300:CDS
AAGGTGTGTGTAACTGGAGC+AGG 0.564196 8.2:-38262643 MS.gene25300:CDS
ATGAGAATCACAAGGCAGAC+GGG 0.568344 8.2:-38260006 MS.gene25300:CDS
GGAGATAATGGAAACAAGTG+GGG 0.572345 8.2:-38262683 None:intergenic
ATTGGTCCCAAAACATGTGT+AGG 0.579319 8.2:+38260511 None:intergenic
GTGTGTGTAACTGGAGCAGG+TGG 0.580174 8.2:-38262640 MS.gene25300:CDS
TATGAGAATCACAAGGCAGA+CGG 0.581333 8.2:-38260007 MS.gene25300:CDS
GAGGAGATAATGGAAACAAG+TGG 0.583352 8.2:-38262685 None:intergenic
AAGGTACAGGGCTAGCAACA+TGG 0.584108 8.2:+38261135 None:intergenic
TGCTAGCAGCTATATTGAGA+TGG 0.585082 8.2:-38259385 MS.gene25300:intron
GGGTGTTAATAACAATAAGA+AGG 0.597736 8.2:-38262662 MS.gene25300:CDS
GTGATTGAGCCTGCTGTGAA+GGG 0.614167 8.2:-38260956 MS.gene25300:CDS
AGCCTGTCTCAAAGCTAATG+TGG 0.618834 8.2:-38260915 MS.gene25300:CDS
GTACAACTGTTGAAGGTACA+GGG 0.621515 8.2:+38261123 None:intergenic
TTCTGTTGAGAGCTATAAGG+AGG 0.627623 8.2:-38259283 MS.gene25300:CDS
GAGATAATGGAAACAAGTGG+GGG 0.650626 8.2:-38262682 None:intergenic
ACTTATTCTCGAATGAGTCA+CGG 0.655492 8.2:+38260080 None:intergenic
GAAAAGAACTGGACTTAACG+TGG 0.670351 8.2:-38260545 MS.gene25300:CDS
AACAGTTGTACCTAATCCTG+AGG 0.672409 8.2:-38261111 MS.gene25300:intron
TTTGGCTTATGAGAATCACA+AGG 0.712000 8.2:-38260014 MS.gene25300:CDS
TGTGTGTAACTGGAGCAGGT+GGG 0.782982 8.2:-38262639 MS.gene25300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAATTAATTAATTAATG+AGG - chr8.2:38262182-38262201 MS.gene25300:intron 10.0%
!! AAGAATTTCATTACTATATA+TGG - chr8.2:38259539-38259558 MS.gene25300:intron 15.0%
!! ATGAATTTCATTACTATATA+TGG - chr8.2:38260020-38260039 MS.gene25300:CDS 15.0%
!! CTGATAATATTTAAATTTAG+AGG + chr8.2:38262082-38262101 None:intergenic 15.0%
!! GGATTAAATATATATTTGTT+GGG + chr8.2:38260283-38260302 None:intergenic 15.0%
!! TAATATTTAAATTTAGAGGT+TGG + chr8.2:38262078-38262097 None:intergenic 15.0%
!! TGAAATTAGTTAAACTATAA+TGG + chr8.2:38261787-38261806 None:intergenic 15.0%
!!! AAAAATAAAAAGTTTCGTTT+TGG - chr8.2:38260357-38260376 MS.gene25300:intron 15.0%
!!! AAAATAAAAAGTTTCGTTTT+GGG - chr8.2:38260358-38260377 MS.gene25300:intron 15.0%
!!! AGAAGTTCTAAAAAAATTTA+TGG - chr8.2:38259614-38259633 MS.gene25300:intron 15.0%
!!! GATTAAATATATATTTGTTG+GGG + chr8.2:38260282-38260301 None:intergenic 15.0%
!!! TTTTTAATACGAATTTGATA+AGG + chr8.2:38261519-38261538 None:intergenic 15.0%
!! AAGAGTTTCATTACTATATA+TGG - chr8.2:38259859-38259878 MS.gene25300:intron 20.0%
!! ACTATATATGGAAAATAACT+AGG - chr8.2:38259551-38259570 MS.gene25300:intron 20.0%
!! ATCTCAAATATATACATACA+GGG + chr8.2:38260304-38260323 None:intergenic 20.0%
!! GGGATTAAATATATATTTGT+TGG + chr8.2:38260284-38260303 None:intergenic 20.0%
!! TTTAACAAGTGTTCATATTT+CGG + chr8.2:38260685-38260704 None:intergenic 20.0%
!!! ATTTTTCAAAGGTCATATAT+AGG + chr8.2:38260513-38260532 None:intergenic 20.0%
!!! CTGTTTTATCAAATTATCAT+TGG - chr8.2:38262441-38262460 MS.gene25300:intron 20.0%
!!! TCATTTAGACAATTAGATTT+TGG + chr8.2:38260130-38260149 None:intergenic 20.0%
! AAAAAATAGAAGAATGCACA+AGG + chr8.2:38261607-38261626 None:intergenic 25.0%
! AAAATCATGTCTTTCTTAGA+AGG - chr8.2:38261833-38261852 MS.gene25300:intron 25.0%
! ACTATATATGGAAAGAAACT+AGG - chr8.2:38259711-38259730 MS.gene25300:intron 25.0%
! ACTGTTAATGTCAAAAACAT+AGG - chr8.2:38260249-38260268 MS.gene25300:intron 25.0%
! ACTTTATCTTTCGGTAAATT+AGG + chr8.2:38261091-38261110 None:intergenic 25.0%
! AGATATCTTGGATTATGAAT+CGG - chr8.2:38260744-38260763 MS.gene25300:intron 25.0%
! ATTAGTTAAACTATAATGGC+AGG + chr8.2:38261783-38261802 None:intergenic 25.0%
! CAGTGTTTATATAGATTTGA+TGG - chr8.2:38262502-38262521 MS.gene25300:intron 25.0%
! CATCTCAAATATATACATAC+AGG + chr8.2:38260305-38260324 None:intergenic 25.0%
! CTTTATCTTTCGGTAAATTA+GGG + chr8.2:38261090-38261109 None:intergenic 25.0%
! TCATCAATCACTTTATCTTT+CGG + chr8.2:38261100-38261119 None:intergenic 25.0%
! TTTGAATAGAATTGTTGAAG+AGG - chr8.2:38260573-38260592 MS.gene25300:CDS 25.0%
!! ACTCTCATCAAACTTTTAAA+AGG - chr8.2:38261479-38261498 MS.gene25300:intron 25.0%
!! ATATGAACACTTGTTAAAAC+TGG - chr8.2:38260687-38260706 MS.gene25300:intron 25.0%
!! TTAATGAGGAGTATATGTAA+AGG - chr8.2:38262196-38262215 MS.gene25300:intron 25.0%
!!! TATAGAAGTTTTGTAACACA+AGG + chr8.2:38261579-38261598 None:intergenic 25.0%
!!! TTGATTTTAATCACATGTGT+AGG + chr8.2:38262416-38262435 None:intergenic 25.0%
AAAATGAAGCTTACTTCGTA+GGG + chr8.2:38262036-38262055 None:intergenic 30.0%
AACATTAGAAATGTTACCTC+AGG + chr8.2:38260844-38260863 None:intergenic 30.0%
AATTATGAATATGCCCTTCA+TGG - chr8.2:38261731-38261750 MS.gene25300:intron 30.0%
AGTTCTATGATGGTCATAAA+TGG + chr8.2:38260210-38260229 None:intergenic 30.0%
ATACTTGAAGCTTAATATGC+AGG - chr8.2:38260952-38260971 MS.gene25300:CDS 30.0%
ATGGAAAATAACTAGGCTTA+AGG - chr8.2:38259558-38259577 MS.gene25300:intron 30.0%
ATTATGAATATGCCCTTCAT+GGG - chr8.2:38261732-38261751 MS.gene25300:intron 30.0%
ATTTGATAAGGCATATCAGT+AGG + chr8.2:38261507-38261526 None:intergenic 30.0%
CACTATCATCATACAAGATT+TGG - chr8.2:38261973-38261992 MS.gene25300:intron 30.0%
GACACTACTATCATATCATA+AGG + chr8.2:38261211-38261230 None:intergenic 30.0%
GAGATATCAACAATTGTATC+AGG - chr8.2:38262245-38262264 MS.gene25300:intron 30.0%
GGGTGTTAATAACAATAAGA+AGG - chr8.2:38259274-38259293 MS.gene25300:CDS 30.0%
GGTTGAAAACAAAGCATAAA+GGG + chr8.2:38260480-38260499 None:intergenic 30.0%
TATCATCATACAAGATTTGG+TGG - chr8.2:38261976-38261995 MS.gene25300:intron 30.0%
TGGAATAATAATACCAGTTC+TGG + chr8.2:38261337-38261356 None:intergenic 30.0%
TGGTTGAAAACAAAGCATAA+AGG + chr8.2:38260481-38260500 None:intergenic 30.0%
TTTGTGGTTGACTGTAAAAT+TGG + chr8.2:38261446-38261465 None:intergenic 30.0%
! ATTTGTCCTACACATGTTTT+GGG - chr8.2:38261419-38261438 MS.gene25300:intron 30.0%
! TTTTAGCCTCTATAAGTGTA+TGG - chr8.2:38261623-38261642 MS.gene25300:intron 30.0%
! TTTTGCAAGTTGCTACATAT+TGG + chr8.2:38260160-38260179 None:intergenic 30.0%
!! AAAGTTTAGGTTGGAAATTC+AGG - chr8.2:38262607-38262626 MS.gene25300:CDS 30.0%
!!! CAAGTATTCAGACTTTTTCA+TGG + chr8.2:38260939-38260958 None:intergenic 30.0%
ACTCTTCAAAGCAGATATCT+TGG - chr8.2:38260732-38260751 MS.gene25300:intron 35.0%
ACTTATTCTCGAATGAGTCA+CGG + chr8.2:38261859-38261878 None:intergenic 35.0%
AGAAACTGCAAAGTTTAGGT+TGG - chr8.2:38262598-38262617 MS.gene25300:CDS 35.0%
AGAAATGTTACCTCAGGATT+AGG + chr8.2:38260838-38260857 None:intergenic 35.0%
AGACTACTGCAAGAATACTA+AGG - chr8.2:38261138-38261157 MS.gene25300:CDS 35.0%
AGTCAAGCTACTTGTATTTG+TGG + chr8.2:38261462-38261481 None:intergenic 35.0%
ATATGAAGCATAGACACCTT+CGG + chr8.2:38260438-38260457 None:intergenic 35.0%
ATGAGATGAGAAATAGTACC+AGG + chr8.2:38259392-38259411 None:intergenic 35.0%
ATGGAAAGAAACTAGGCTTA+AGG - chr8.2:38259718-38259737 MS.gene25300:intron 35.0%
ATGTCGTGTTTACTAACTTG+AGG + chr8.2:38260916-38260935 None:intergenic 35.0%
CAAAACATGTGTAGGACAAA+TGG + chr8.2:38261420-38261439 None:intergenic 35.0%
CAAAGGTCATATATAGGTCA+TGG + chr8.2:38260507-38260526 None:intergenic 35.0%
GAAAATGAAGCTTACTTCGT+AGG + chr8.2:38262037-38262056 None:intergenic 35.0%
GTTAAGCTTCTTCTTTCCAA+AGG - chr8.2:38259338-38259357 MS.gene25300:CDS 35.0%
TCAGAGAAACTGCAAAGTTT+AGG - chr8.2:38262594-38262613 MS.gene25300:CDS 35.0%
TCATATATAGGTCATGGAGT+TGG + chr8.2:38260501-38260520 None:intergenic 35.0%
TCGAAAGAATGAGAGAAAAG+AGG + chr8.2:38260610-38260629 None:intergenic 35.0%
TCTATGATGGTCATAAATGG+AGG + chr8.2:38260207-38260226 None:intergenic 35.0%
TGATTCTGTTGAGAGCTATA+AGG - chr8.2:38262650-38262669 MS.gene25300:CDS 35.0%
TTATGAATATGCCCTTCATG+GGG - chr8.2:38261733-38261752 MS.gene25300:intron 35.0%
! AAGTGATTGATGAGTCTTGT+TGG - chr8.2:38261107-38261126 MS.gene25300:intron 35.0%
! AATAAGAAGGTGTGTGTAAC+TGG - chr8.2:38259287-38259306 MS.gene25300:CDS 35.0%
! AGTTCTTTTCGCGAAATTCA+GGG + chr8.2:38261381-38261400 None:intergenic 35.0%
! CATTTGTCCTACACATGTTT+TGG - chr8.2:38261418-38261437 MS.gene25300:intron 35.0%
! GGCTTAAGGACATGTAATTT+TGG - chr8.2:38259572-38259591 MS.gene25300:intron 35.0%
! TCTTTTCTCTCATTCTTTCG+AGG - chr8.2:38260609-38260628 MS.gene25300:intron 35.0%
! TTTGGCTTATGAGAATCACA+AGG - chr8.2:38261922-38261941 MS.gene25300:intron 35.0%
!! CGTAGTTGATGCAATACTTT+TGG - chr8.2:38261904-38261923 MS.gene25300:intron 35.0%
!! CTGTTGAGCAGATTTTTCAA+AGG + chr8.2:38260524-38260543 None:intergenic 35.0%
!! TATCTGCTTTGAAGAGTGTA+AGG + chr8.2:38260729-38260748 None:intergenic 35.0%
AACACACGTTAGACACTTCT+TGG + chr8.2:38260331-38260350 None:intergenic 40.0%
AACAGTTGTACCTAATCCTG+AGG - chr8.2:38260825-38260844 MS.gene25300:CDS 40.0%
ACCATCATAGAACTCTGACA+CGG - chr8.2:38260216-38260235 MS.gene25300:intron 40.0%
AGTGTATGTCTGAAAACTGC+AGG - chr8.2:38260655-38260674 MS.gene25300:intron 40.0%
ATTGGTCCCAAAACATGTGT+AGG + chr8.2:38261428-38261447 None:intergenic 40.0%
CAGAGAAAAATCCCCATGAA+GGG + chr8.2:38261747-38261766 None:intergenic 40.0%
CATTCGAGAATAAGTGTGCT+TGG - chr8.2:38261864-38261883 MS.gene25300:intron 40.0%
CTGAATTTCGCGAAAAGAAC+TGG - chr8.2:38261380-38261399 MS.gene25300:intron 40.0%
CTTGAAGCTTAATATGCAGG+TGG - chr8.2:38260955-38260974 MS.gene25300:CDS 40.0%
GAAAAGAACTGGACTTAACG+TGG - chr8.2:38261391-38261410 MS.gene25300:intron 40.0%
GGATTAGGTACAACTGTTGA+AGG + chr8.2:38260823-38260842 None:intergenic 40.0%
GTATGGCCATACACTTATAG+AGG + chr8.2:38261632-38261651 None:intergenic 40.0%
TAATTTGATATGTCACCGCG+TGG - chr8.2:38260879-38260898 MS.gene25300:CDS 40.0%
TATGAAGCATAGACACCTTC+GGG + chr8.2:38260437-38260456 None:intergenic 40.0%
TATGAGAATCACAAGGCAGA+CGG - chr8.2:38261929-38261948 MS.gene25300:intron 40.0%
TCAATGAGTGTTTCCTCCAA+TGG + chr8.2:38262633-38262652 None:intergenic 40.0%
TCCATGGACAAAGTAACCTT+TGG + chr8.2:38259357-38259376 None:intergenic 40.0%
TCGGTTTACTCTGCAATTGT+TGG - chr8.2:38260763-38260782 MS.gene25300:intron 40.0%
TCTTTCGAGGTATGAGTGTT+GGG - chr8.2:38260622-38260641 MS.gene25300:intron 40.0%
TGCTAGCAGCTATATTGAGA+TGG - chr8.2:38262551-38262570 MS.gene25300:intron 40.0%
TTCTGTTGAGAGCTATAAGG+AGG - chr8.2:38262653-38262672 MS.gene25300:CDS 40.0%
TTCTTTCGAGGTATGAGTGT+TGG - chr8.2:38260621-38260640 MS.gene25300:intron 40.0%
! CAATGTAGCATCGAAACTTC+AGG - chr8.2:38262354-38262373 MS.gene25300:intron 40.0%
! CAGTTCTTTTCGCGAAATTC+AGG + chr8.2:38261382-38261401 None:intergenic 40.0%
! GTACAACTGTTGAAGGTACA+GGG + chr8.2:38260816-38260835 None:intergenic 40.0%
! TCCAAAGGTTACTTTGTCCA+TGG - chr8.2:38259353-38259372 MS.gene25300:CDS 40.0%
! TTCGTAGGGTACTTGTAGTT+TGG + chr8.2:38262022-38262041 None:intergenic 40.0%
! TTGAAGGTGTATCTTGTGTC+CGG - chr8.2:38262377-38262396 MS.gene25300:intron 40.0%
!! TTCCACATTAGCTTTGAGAC+AGG + chr8.2:38261026-38261045 None:intergenic 40.0%
AAAACACGATTGCGGTGAAG+CGG + chr8.2:38261661-38261680 None:intergenic 45.0%
AACACTGACACATTCACTGC+TGG + chr8.2:38262489-38262508 None:intergenic 45.0%
AGCCTGTCTCAAAGCTAATG+TGG - chr8.2:38261021-38261040 MS.gene25300:intron 45.0%
AGGTTGGAAATTCAGGCCAT+TGG - chr8.2:38262614-38262633 MS.gene25300:CDS 45.0%
ATGAGAATCACAAGGCAGAC+GGG - chr8.2:38261930-38261949 MS.gene25300:intron 45.0%
CAAGACAGAAGCAGAAGAAC+AGG - chr8.2:38261355-38261374 MS.gene25300:intron 45.0%
CGTCACATATGATCCAGAAC+TGG - chr8.2:38261321-38261340 MS.gene25300:intron 45.0%
CTGTTCTTCTGCTTCTGTCT+TGG + chr8.2:38261357-38261376 None:intergenic 45.0%
GCAGAGAAAAATCCCCATGA+AGG + chr8.2:38261748-38261767 None:intergenic 45.0%
GTAGAGTCATGAGAATGAGC+AGG - chr8.2:38262274-38262293 MS.gene25300:intron 45.0%
TCCGTGTCAGAGTTCTATGA+TGG + chr8.2:38260220-38260239 None:intergenic 45.0%
TTGCAAGTAATGTTGCGCGA+TGG - chr8.2:38262302-38262321 MS.gene25300:intron 45.0%
TTGGAAATTCAGGCCATTGG+AGG - chr8.2:38262617-38262636 MS.gene25300:CDS 45.0%
! CTTCACCGCAATCGTGTTTT+GGG - chr8.2:38261661-38261680 MS.gene25300:intron 45.0%
! GGTACAACTGTTGAAGGTAC+AGG + chr8.2:38260817-38260836 None:intergenic 45.0%
!! GCATCGAAACTTCAGGTTGA+AGG - chr8.2:38262361-38262380 MS.gene25300:intron 45.0%
!! TAAAAAAATTGAAAATTATT+AGG + chr8.2:38259419-38259438 None:intergenic 5.0%
AAACACGATTGCGGTGAAGC+GGG + chr8.2:38261660-38261679 None:intergenic 50.0%
AAGGTGTGTGTAACTGGAGC+AGG - chr8.2:38259293-38259312 MS.gene25300:CDS 50.0%
AGTGATTGAGCCTGCTGTGA+AGG - chr8.2:38260979-38260998 MS.gene25300:intron 50.0%
CAGGCTCTCTAACAGTTCCA+TGG + chr8.2:38259373-38259392 None:intergenic 50.0%
CATGGAACTGTTAGAGAGCC+TGG - chr8.2:38259371-38259390 MS.gene25300:CDS 50.0%
CGACTCCCAAAACACGATTG+CGG + chr8.2:38261669-38261688 None:intergenic 50.0%
GAGGCAAAACTAGAACCACG+CGG + chr8.2:38260897-38260916 None:intergenic 50.0%
GTGATTGAGCCTGCTGTGAA+GGG - chr8.2:38260980-38260999 MS.gene25300:intron 50.0%
GTTGAGAGCTATAAGGAGGC+TGG - chr8.2:38262657-38262676 MS.gene25300:CDS 50.0%
TGTGTGTAACTGGAGCAGGT+GGG - chr8.2:38259297-38259316 MS.gene25300:CDS 50.0%
TTAGCAGTTCCCTTCACAGC+AGG + chr8.2:38260992-38261011 None:intergenic 50.0%
! AAGGTACAGGGCTAGCAACA+TGG + chr8.2:38260804-38260823 None:intergenic 50.0%
! CAGGTGGGTTTGTAGCATCA+TGG - chr8.2:38259312-38259331 MS.gene25300:CDS 50.0%
! GCTTCACCGCAATCGTGTTT+TGG - chr8.2:38261660-38261679 MS.gene25300:intron 50.0%
! TGTAGGTATCGTCTCGATGC+CGG + chr8.2:38262399-38262418 None:intergenic 50.0%
!!! CGCAATCGTGTTTTGGGAGT+CGG - chr8.2:38261667-38261686 MS.gene25300:intron 50.0%
ACAGAGACGCGAAATCCCGA+AGG - chr8.2:38260419-38260438 MS.gene25300:intron 55.0%
GTGTGTGTAACTGGAGCAGG+TGG - chr8.2:38259296-38259315 MS.gene25300:CDS 55.0%
GATTGCGGTGAAGCGGGTAG+TGG + chr8.2:38261654-38261673 None:intergenic 60.0%
! CGGTGAAGCGGGTAGTGGTA+TGG + chr8.2:38261649-38261668 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 38259260 38262698 38259260 ID=MS.gene25300
chr8.2 mRNA 38259260 38262698 38259260 ID=MS.gene25300.t1;Parent=MS.gene25300
chr8.2 exon 38262566 38262698 38262566 ID=MS.gene25300.t1.exon1;Parent=MS.gene25300.t1
chr8.2 CDS 38262566 38262698 38262566 ID=cds.MS.gene25300.t1;Parent=MS.gene25300.t1
chr8.2 exon 38261112 38261281 38261112 ID=MS.gene25300.t1.exon2;Parent=MS.gene25300.t1
chr8.2 CDS 38261112 38261281 38261112 ID=cds.MS.gene25300.t1;Parent=MS.gene25300.t1
chr8.2 exon 38260799 38260984 38260799 ID=MS.gene25300.t1.exon3;Parent=MS.gene25300.t1
chr8.2 CDS 38260799 38260984 38260799 ID=cds.MS.gene25300.t1;Parent=MS.gene25300.t1
chr8.2 exon 38260458 38260620 38260458 ID=MS.gene25300.t1.exon4;Parent=MS.gene25300.t1
chr8.2 CDS 38260458 38260620 38260458 ID=cds.MS.gene25300.t1;Parent=MS.gene25300.t1
chr8.2 exon 38259917 38260103 38259917 ID=MS.gene25300.t1.exon5;Parent=MS.gene25300.t1
chr8.2 CDS 38259917 38260103 38259917 ID=cds.MS.gene25300.t1;Parent=MS.gene25300.t1
chr8.2 exon 38259260 38259398 38259260 ID=MS.gene25300.t1.exon6;Parent=MS.gene25300.t1
chr8.2 CDS 38259260 38259398 38259260 ID=cds.MS.gene25300.t1;Parent=MS.gene25300.t1
Gene Sequence

>MS.gene25300

ATGGAAACAAGTGGGGGTGTTAATAACAATAAGAAGGTGTGTGTAACTGGAGCAGGTGGGTTTGTAGCATCATGGCTTGTTAAGCTTCTTCTTTCCAAAGGTTACTTTGTCCATGGAACTGTTAGAGAGCCTGGTACTATTTCTCATCTCATTTTTCCTAATAATTTTCAATTTTTTTAGAATTAATTAAAAATATCTAGTTATTATTGAAAAATATTCTAATACATTCATTAGTTGTTAATATTATATATGTATTGTTTAAGTTTATTTTAATAAGCAAAGAATTTCATTACTATATATGGAAAATAACTAGGCTTAAGGACATGTAATTTTGGTGCTGTAAGAGAGATCAAGAGAAGTTCTAAAAAAATTTATATTCTAATACATTCATTAGATGTTAATGTTATATATGTATTGTTTAAGTTTATTTTAATAAGCAAAGAATTTCATTACTATATATGGAAAGAAACTAGGCTTAAGGACATGTAATTTTGATGCTGTAAGAGAGATCAAGAGAAGTTCTAAATTTTTTTATATTCTAATACATTCATTAGATGTTAATGTTATATATGTATTGTTTAAGTTTATTTTAATAAGCAAAGAGTTTCATTACTATATATGGAAAGAAACTAGGCTTAAGGACATGTAATTTTGATGCTGTAAGAGAGATCAAGAGAAGTTCTAAAAAAAATTTATATTCTAATACATTCATTAGATGTTAATGTTATATATGTATTGTTTAAGTTTATTTTAATAAGCAATGAATTTCATTACTATATATGGAAAGAAACTAGGCTTAAGGACATGTAATTTTGATGCTGTAAGAGAGATCAAGAGAAGTTCTAAAAAAATTTATGGTGATTCAATCCAAAATCTAATTGTCTAAATGAAAGTCAACCAATATGTAGCAACTTGCAAAACTAGAGCAAATTAACAATATATTTCCTCCATTTATGACCATCATAGAACTCTGACACGGACAAGTCAACACTGTTAATGTCAAAAACATAGGACACTGACCCCAACAAATATATATTTAATCCCTGTATGTATATATTTGAGATGTGTCCAAGAAGTGTCTAACGTGTGTTGATACAAAAAATAAAAAGTTTCGTTTTGGGACACTTTTCGTACGAGTGTCAGACTAACTCATGTCAACACAGAGACGCGAAATCCCGAAGGTGTCTATGCTTCATATATCATACGAGAAGTGAGAACCCTTTATGCTTTGTTTTCAACCAACTCCATGACCTATATATGACCTTTGAAAAATCTGCTCAACAGTTACAATTTCAAATTACTTATTATACTATTTTGAATAGAATTGTTGAAGAGGATGTTGTTATGCCTCTTTTCTCTCATTCTTTCGAGGTATGAGTGTTGGGATTCATTTGAAGTGTATGTCTGAAAACTGCAGGTAGTCCGAAATATGAACACTTGTTAAAACTGGAGAAAGCTTCTGAGAACCTTACACTCTTCAAAGCAGATATCTTGGATTATGAATCGGTTTACTCTGCAATTGTTGGATGCAGTGCAGTTTTCCATGTTGCTAGCCCTGTACCTTCAACAGTTGTACCTAATCCTGAGGTAACATTTCTAATGTTCTCTTATATGCATACTAATTTGATATGTCACCGCGTGGTTCTAGTTTTGCCTCAAGTTAGTAAACACGACATCCATGAAAAAGTCTGAATACTTGAAGCTTAATATGCAGGTGGAAGTGATTGAGCCTGCTGTGAAGGGAACTGCTAATGTACTTGAAGCCTGTCTCAAAGCTAATGTGGAACGTGTCGTCTTTGTATCATCTGCTGCTGCTGTTATTATCAACCCTAATTTACCGAAAGATAAAGTGATTGATGAGTCTTGTTGGTCTGACAAAGACTACTGCAAGAATACTAAGGTCACTAAGATTTTATTATATTTGTGCATGACATGAACTTATCATTAACCTTATGATATGATAGTAGTGTCTGTTATACTATTTTTATTCAGATCATTAAGTTTCGAAATATGTTATGTTTCAATCATCAAAGAATTTTGTGTTATGTAAATAAACTTATGCGTCACATATGATCCAGAACTGGTATTATTATTCCAAGACAGAAGCAGAAGAACAGGCCCTGAATTTCGCGAAAAGAACTGGACTTAACGTGGTAACCATTTGTCCTACACATGTTTTGGGACCAATTTTACAGTCAACCACAAATACAAGTAGCTTGACTCTCATCAAACTTTTAAAAGGTACCTACTGATATGCCTTATCAAATTCGTATTAAAAAATGCATATTTTTGAACTGTACAATGCGAGTTAAAATTCCTTGTGTTACAAAACTTCTATAAAATTCCTTGTGCATTCTTCTATTTTTTAGCCTCTATAAGTGTATGGCCATACCACTACCCGCTTCACCGCAATCGTGTTTTGGGAGTCGGCTGCTATGCTACGCTATCTGCTGCTAACTAGATAGATCATGAATTATGAATATGCCCTTCATGGGGATTTTTCTCTGCATAACTTGTTTTCCTGCCATTATAGTTTAACTAATTTCATACATTATAGATTTCATATAAATATTAAAATCATGTCTTTCTTAGAAGGCCGTGACTCATTCGAGAATAAGTGTGCTTGGATTGTTGATGTACGAGACGTAGTTGATGCAATACTTTTGGCTTATGAGAATCACAAGGCAGACGGGAGATACATCTGCACTTCACACACTATCATCATACAAGATTTGGTGGAGAGATTGAAGAGTATATATCCAAACTACAAGTACCCTACGAAGTAAGCTTCATTTTCTAAAGTAGTAGTCATAAACCAACCTCTAAATTTAAATATTATCAGTAATATTAATAATAGTAAGATGTATAAGAAATATGTAAGAAATGTGAAAAGAAAGAATTTTTTGCAACAGAAAACTAATGAAGTAATTAATTAATTAATGAGGAGTATATGTAAAGGAGATTAACTGTTGAGCATGTTCTTGTGAGATATCAACAATTGTATCAGGAAAATTGTAGAGTCATGAGAATGAGCAGGAAAAATTGCAAGTAATGTTGCGCGATGGAGTACTAGCAGTGTCAATATTAATAGTTTCAATGTAGCATCGAAACTTCAGGTTGAAGGTGTATCTTGTGTCCGGCATCGAGACGATACCTACACATGTGATTAAAATCAATACTTCTGTTTTATCAAATTATCATTGGTGTCAGTGTGTCAATGTCGTGTCCAGCAGTGAATGTGTCAGTGTTTATATAGATTTGATGGTTACAATCTTATGTTAAAGTTTTCGTTGCTAGCAGCTATATTGAGATGGATGATTACAAAATGCTGAGCTCAGAGAAACTGCAAAGTTTAGGTTGGAAATTCAGGCCATTGGAGGAAACACTCATTGATTCTGTTGAGAGCTATAAGGAGGCTGGACTACTTCAATCTGAATAA
Protein sequence

>MS.gene25300.t1

METSGGVNNNKKVCVTGAGGFVASWLVKLLLSKGYFVHGTVREPGSPKYEHLLKLEKASENLTLFKADILDYESVYSAIVGCSAVFHVASPVPSTVVPNPEVEVIEPAVKGTANVLEACLKANVERVVFVSSAAAVIINPNLPKDKVIDESCWSDKDYCKNTKNWYYYSKTEAEEQALNFAKRTGLNVVTICPTHVLGPILQSTTNTSSLTLIKLLKGRDSFENKCAWIVDVRDVVDAILLAYENHKADGRYICTSHTIIIQDLVERLKSIYPNYKYPTNYIEMDDYKMLSSEKLQSLGWKFRPLEETLIDSVESYKEAGLLQSE