Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25632.t1 | XP_013445572.1 | 99.1 | 109 | 1 | 0 | 1 | 109 | 1 | 109 | 1.90E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25632.t1 | O96860 | 56.2 | 89 | 37 | 1 | 1 | 89 | 1 | 87 | 4.6e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25632.t1 | A0A072TRW8 | 99.1 | 109 | 1 | 0 | 1 | 109 | 1 | 109 | 1.4e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053073 | MS.gene25632 | 0.804984 | 1.66E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene25632 | 0.801696 | 7.97E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene25632 | 0.81026 | 1.26E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene25632 | MS.gene035362 | PPI |
| MS.gene25632 | MS.gene041598 | PPI |
| MS.gene25632 | MS.gene77822 | PPI |
| MS.gene25632 | MS.gene035363 | PPI |
| MS.gene25632 | MS.gene78481 | PPI |
| MS.gene25632 | MS.gene041599 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25632.t1 | MTR_8g464690 | 99.083 | 109 | 1 | 0 | 1 | 109 | 1 | 109 | 3.04e-77 | 223 |
| MS.gene25632.t1 | MTR_6g087770 | 51.087 | 92 | 45 | 0 | 1 | 92 | 1 | 92 | 3.67e-30 | 103 |
| MS.gene25632.t1 | MTR_2g101850 | 55.556 | 90 | 40 | 0 | 1 | 90 | 1 | 90 | 1.25e-29 | 102 |
| MS.gene25632.t1 | MTR_4g120150 | 48.837 | 86 | 42 | 1 | 5 | 90 | 46 | 129 | 3.04e-26 | 94.7 |
| MS.gene25632.t1 | MTR_4g114060 | 49.367 | 79 | 40 | 0 | 11 | 89 | 159 | 237 | 5.08e-22 | 87.0 |
| MS.gene25632.t1 | MTR_8g104900 | 45.882 | 85 | 44 | 1 | 12 | 94 | 45 | 129 | 1.54e-19 | 77.8 |
| MS.gene25632.t1 | MTR_1g031880 | 43.529 | 85 | 46 | 1 | 12 | 94 | 59 | 143 | 2.62e-19 | 77.4 |
| MS.gene25632.t1 | MTR_4g107350 | 50.633 | 79 | 38 | 1 | 12 | 89 | 222 | 300 | 1.74e-18 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25632.t1 | AT4G27360 | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 1.64e-50 | 155 |
| MS.gene25632.t1 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 4.74e-33 | 110 |
| MS.gene25632.t1 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 4.74e-33 | 110 |
| MS.gene25632.t1 | AT1G52245 | 51.064 | 94 | 46 | 0 | 1 | 94 | 1 | 94 | 3.18e-30 | 103 |
| MS.gene25632.t1 | AT4G15930 | 49.412 | 85 | 41 | 1 | 5 | 89 | 39 | 121 | 3.46e-24 | 89.4 |
| MS.gene25632.t1 | AT5G20110 | 46.988 | 83 | 44 | 0 | 12 | 94 | 123 | 205 | 2.69e-21 | 84.3 |
| MS.gene25632.t1 | AT1G23220 | 39.773 | 88 | 52 | 1 | 8 | 94 | 38 | 125 | 3.98e-18 | 74.3 |
| MS.gene25632.t1 | AT4G15930 | 49.180 | 61 | 29 | 1 | 5 | 65 | 39 | 97 | 1.30e-13 | 61.6 |
Find 21 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTAGGCACTGATTT+TGG | 0.221609 | 8.2:+45444944 | MS.gene25632:CDS |
| CAGGAATTTGATAGGGTTCA+TGG | 0.298316 | 8.2:+45444908 | MS.gene25632:intron |
| CAGTTACATCATAGAAATCT+AGG | 0.300776 | 8.2:-45444763 | None:intergenic |
| AATTATTGCAGGAATTTGAT+AGG | 0.304941 | 8.2:+45444900 | MS.gene25632:intron |
| ATCATAGAAATCTAGGGCTT+TGG | 0.353606 | 8.2:-45444756 | None:intergenic |
| CTTCAATTCATCATGCTAGA+AGG | 0.354098 | 8.2:+45444668 | None:intergenic |
| TTGCTCTTTAAGGGATCTGC+TGG | 0.420352 | 8.2:+45445028 | MS.gene25632:CDS |
| CCTGGTTGGCAATGCATTGT+AGG | 0.440795 | 8.2:+45444932 | MS.gene25632:CDS |
| CTAGAAGGCAAGGCAGTGAT+TGG | 0.481296 | 8.2:+45444683 | MS.gene25632:CDS |
| CCTACAATGCATTGCCAACC+AGG | 0.504627 | 8.2:-45444932 | None:intergenic |
| AAATCAGTTCTCAGCAATAG+AGG | 0.512873 | 8.2:+45445066 | MS.gene25632:CDS |
| TATAGCTCGATTTATAAAGA+AGG | 0.514762 | 8.2:+45444796 | MS.gene25632:CDS |
| TTTGATAGGGTTCATGGACC+TGG | 0.522418 | 8.2:+45444914 | MS.gene25632:CDS |
| ATTATTGCAGGAATTTGATA+GGG | 0.524356 | 8.2:+45444901 | MS.gene25632:intron |
| TATAAATCGAGCTATATCAA+TGG | 0.561045 | 8.2:-45444789 | None:intergenic |
| ATTCATCATGCTAGAAGGCA+AGG | 0.581622 | 8.2:+45444673 | None:intergenic |
| TCCTTTGTGACACATTGTAG+TGG | 0.583602 | 8.2:+45444968 | MS.gene25632:CDS |
| AGTTACATCATAGAAATCTA+GGG | 0.585718 | 8.2:-45444762 | None:intergenic |
| ATAGGGTTCATGGACCTGGT+TGG | 0.594187 | 8.2:+45444918 | MS.gene25632:CDS |
| GCCACTACAATGTGTCACAA+AGG | 0.659746 | 8.2:-45444969 | None:intergenic |
| AGATTTCTATGATGTAACTG+AGG | 0.680263 | 8.2:+45444766 | MS.gene25632:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTAATAAATTAAAACTAAG+AGG | + | chr8.2:45444817-45444836 | MS.gene25632:intron | 20.0% |
| ! | AAACTAAGAGGATTGTTTAT+TGG | + | chr8.2:45444829-45444848 | MS.gene25632:intron | 25.0% |
| ! | AATTATTGCAGGAATTTGAT+AGG | + | chr8.2:45444900-45444919 | MS.gene25632:intron | 25.0% |
| ! | AGTTACATCATAGAAATCTA+GGG | - | chr8.2:45444765-45444784 | None:intergenic | 25.0% |
| ! | ATGTGTTAGTTAATTATTGC+AGG | + | chr8.2:45444889-45444908 | MS.gene25632:intron | 25.0% |
| ! | ATTATTGCAGGAATTTGATA+GGG | + | chr8.2:45444901-45444920 | MS.gene25632:intron | 25.0% |
| ! | TATAAATCGAGCTATATCAA+TGG | - | chr8.2:45444792-45444811 | None:intergenic | 25.0% |
| ! | TATAGCTCGATTTATAAAGA+AGG | + | chr8.2:45444796-45444815 | MS.gene25632:CDS | 25.0% |
| !!! | TGCTTCATCTATTTTTGTAT+AGG | + | chr8.2:45444992-45445011 | MS.gene25632:CDS | 25.0% |
| AGATTTCTATGATGTAACTG+AGG | + | chr8.2:45444766-45444785 | MS.gene25632:CDS | 30.0% | |
| CAGTTACATCATAGAAATCT+AGG | - | chr8.2:45444766-45444785 | None:intergenic | 30.0% | |
| ! | ATGGCAATTTTGCTCTTTAA+GGG | + | chr8.2:45445019-45445038 | MS.gene25632:CDS | 30.0% |
| !!! | CTATTTTTGTATAGGTAGCA+TGG | + | chr8.2:45445000-45445019 | MS.gene25632:CDS | 30.0% |
| AAATCAGTTCTCAGCAATAG+AGG | + | chr8.2:45445066-45445085 | MS.gene25632:CDS | 35.0% | |
| ! | ATCATAGAAATCTAGGGCTT+TGG | - | chr8.2:45444759-45444778 | None:intergenic | 35.0% |
| ! | CATGGCAATTTTGCTCTTTA+AGG | + | chr8.2:45445018-45445037 | MS.gene25632:CDS | 35.0% |
| !! | AACTGATTTTCTTCTGCTTG+TGG | - | chr8.2:45445055-45445074 | None:intergenic | 35.0% |
| CAGGAATTTGATAGGGTTCA+TGG | + | chr8.2:45444908-45444927 | MS.gene25632:intron | 40.0% | |
| TCCTTTGTGACACATTGTAG+TGG | + | chr8.2:45444968-45444987 | MS.gene25632:CDS | 40.0% | |
| !!! | TGCATTGTAGGCACTGATTT+TGG | + | chr8.2:45444944-45444963 | MS.gene25632:CDS | 40.0% |
| GCCACTACAATGTGTCACAA+AGG | - | chr8.2:45444972-45444991 | None:intergenic | 45.0% | |
| TTTGATAGGGTTCATGGACC+TGG | + | chr8.2:45444914-45444933 | MS.gene25632:CDS | 45.0% | |
| ! | TTGCTCTTTAAGGGATCTGC+TGG | + | chr8.2:45445028-45445047 | MS.gene25632:CDS | 45.0% |
| !!! | TTTTAATGTAATTTTTTTTA+TGG | + | chr8.2:45444855-45444874 | MS.gene25632:intron | 5.0% |
| ATAGGGTTCATGGACCTGGT+TGG | + | chr8.2:45444918-45444937 | MS.gene25632:CDS | 50.0% | |
| CCTACAATGCATTGCCAACC+AGG | - | chr8.2:45444935-45444954 | None:intergenic | 50.0% | |
| CCTGGTTGGCAATGCATTGT+AGG | + | chr8.2:45444932-45444951 | MS.gene25632:CDS | 50.0% | |
| CTAGAAGGCAAGGCAGTGAT+TGG | + | chr8.2:45444683-45444702 | MS.gene25632:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 45444680 | 45445102 | 45444680 | ID=MS.gene25632 |
| chr8.2 | mRNA | 45444680 | 45445102 | 45444680 | ID=MS.gene25632.t1;Parent=MS.gene25632 |
| chr8.2 | exon | 45444680 | 45444817 | 45444680 | ID=MS.gene25632.t1.exon1;Parent=MS.gene25632.t1 |
| chr8.2 | CDS | 45444680 | 45444817 | 45444680 | ID=cds.MS.gene25632.t1;Parent=MS.gene25632.t1 |
| chr8.2 | exon | 45444911 | 45445102 | 45444911 | ID=MS.gene25632.t1.exon2;Parent=MS.gene25632.t1 |
| chr8.2 | CDS | 45444911 | 45445102 | 45444911 | ID=cds.MS.gene25632.t1;Parent=MS.gene25632.t1 |
| Gene Sequence |
| Protein sequence |
