AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25854

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25854.t1 MTR_3g079560 95.960 495 19 1 1 494 1 495 0.0 961
MS.gene25854.t1 MTR_3g079570 90.927 496 42 3 1 494 1 495 0.0 904
MS.gene25854.t1 MTR_3g079600 87.575 499 55 4 1 494 1 497 0.0 886
MS.gene25854.t1 MTR_3g079590 89.113 496 51 3 1 494 1 495 0.0 881
MS.gene25854.t1 MTR_3g079630 84.615 507 62 6 1 494 1 504 0.0 860
MS.gene25854.t1 MTR_3g079620 85.089 503 64 3 1 493 1 502 0.0 849
MS.gene25854.t1 MTR_1g020090 62.025 474 172 5 18 489 30 497 0.0 591
MS.gene25854.t1 MTR_1g021010 60.861 488 183 7 10 493 7 490 0.0 576
MS.gene25854.t1 MTR_1g021020 57.490 494 202 6 2 493 4 491 0.0 563
MS.gene25854.t1 MTR_3g034290 53.239 494 205 10 11 489 8 490 1.73e-175 503
MS.gene25854.t1 MTR_0003s0560 54.988 431 185 6 65 490 62 488 5.83e-161 466
MS.gene25854.t1 MTR_1g095560 51.570 446 198 6 57 491 20 458 5.49e-159 459
MS.gene25854.t1 MTR_1g095540 52.900 431 190 7 73 491 29 458 1.43e-154 448
MS.gene25854.t1 MTR_1g095560 48.745 478 195 8 57 491 20 490 5.93e-153 445
MS.gene25854.t1 MTR_2g062990 49.535 430 206 5 72 490 34 463 8.89e-152 441
MS.gene25854.t1 MTR_2g062980 50.350 429 202 6 73 490 35 463 1.80e-151 441
MS.gene25854.t1 MTR_2g063000 48.980 441 211 6 62 490 26 464 2.69e-151 440
MS.gene25854.t1 MTR_4g075150 50.993 453 194 12 59 492 21 464 3.59e-151 439
MS.gene25854.t1 MTR_4g075150 50.549 455 195 11 59 492 21 466 3.84e-151 439
MS.gene25854.t1 MTR_2g081620 49.412 425 204 6 73 486 36 460 5.51e-148 432
MS.gene25854.t1 MTR_7g013790 49.315 438 202 5 70 489 29 464 6.79e-145 424
MS.gene25854.t1 MTR_3g034290 52.300 413 170 10 11 408 8 408 2.44e-142 415
MS.gene25854.t1 MTR_4g089155 48.984 443 195 10 74 491 51 487 2.09e-140 413
MS.gene25854.t1 MTR_4g122950 46.637 446 210 6 73 491 35 479 9.54e-140 411
MS.gene25854.t1 MTR_2g012860 47.955 440 204 9 73 490 38 474 6.83e-135 398
MS.gene25854.t1 MTR_2g012850 46.682 437 210 7 75 491 34 467 1.49e-134 397
MS.gene25854.t1 MTR_4g122960 45.270 444 217 7 73 491 35 477 3.48e-132 392
MS.gene25854.t1 MTR_2g063000 44.444 441 200 7 62 490 26 433 3.13e-127 377
MS.gene25854.t1 MTR_3g090130 46.050 443 214 11 69 491 21 458 4.23e-121 363
MS.gene25854.t1 MTR_3g090150 44.344 442 225 9 69 491 26 465 8.11e-118 355
MS.gene25854.t1 MTR_3g498755 44.318 440 219 10 72 489 10 445 4.99e-117 352
MS.gene25854.t1 MTR_5g072570 44.494 445 222 9 69 491 22 463 2.41e-116 351
MS.gene25854.t1 MTR_2g449850 43.341 443 214 11 73 491 35 464 3.99e-113 342
MS.gene25854.t1 MTR_8g099930 42.308 442 225 12 73 491 30 464 1.85e-109 333
MS.gene25854.t1 MTR_0003s0560 59.259 270 107 3 65 334 62 328 3.28e-109 328
MS.gene25854.t1 MTR_7g021130 41.935 434 228 9 75 489 29 457 4.10e-109 332
MS.gene25854.t1 MTR_3g434850 41.216 444 221 11 71 488 25 454 1.00e-106 325
MS.gene25854.t1 MTR_1g040370 39.765 425 214 9 72 489 26 415 1.48e-103 316
MS.gene25854.t1 MTR_7g080180 35.551 481 241 17 69 485 20 495 1.19e-74 244
MS.gene25854.t1 MTR_8g011350 33.833 467 248 9 77 486 31 493 1.59e-73 241
MS.gene25854.t1 MTR_1g102130 35.794 447 217 16 64 483 81 484 9.19e-73 239
MS.gene25854.t1 MTR_7g051430 32.371 485 263 10 58 485 16 492 6.36e-67 223
MS.gene25854.t1 MTR_8g011350 32.805 442 236 9 77 461 31 468 2.86e-63 213
MS.gene25854.t1 MTR_7g080180 34.505 455 222 17 69 458 20 463 5.98e-63 212
MS.gene25854.t1 MTR_1g050545 31.455 426 247 11 89 490 81 485 1.87e-56 196
MS.gene25854.t1 MTR_4g057585 31.519 441 234 15 82 487 87 494 1.11e-55 194
MS.gene25854.t1 MTR_4g021530 30.664 437 264 15 76 483 39 465 1.23e-55 193
MS.gene25854.t1 MTR_4g057595 30.822 438 231 15 91 491 97 499 1.52e-54 191
MS.gene25854.t1 MTR_4g021520 30.682 440 264 14 74 483 37 465 2.87e-53 186
MS.gene25854.t1 MTR_2g095730 30.000 430 255 14 91 494 94 503 1.82e-52 185
MS.gene25854.t1 MTR_1g094155 30.211 427 252 13 88 490 87 491 3.16e-52 184
MS.gene25854.t1 MTR_4g021535 29.773 440 268 16 74 483 33 461 5.07e-52 183
MS.gene25854.t1 MTR_2g018590 29.358 436 275 11 77 483 33 464 5.34e-52 183
MS.gene25854.t1 MTR_2g095750 30.353 425 243 14 91 487 100 499 7.40e-52 184
MS.gene25854.t1 MTR_4g021490 30.206 437 270 15 74 483 37 465 8.49e-52 182
MS.gene25854.t1 MTR_4g021500 30.612 441 261 16 74 482 37 464 1.52e-51 188
MS.gene25854.t1 MTR_4g021500 28.941 425 261 14 89 483 487 900 3.12e-43 164
MS.gene25854.t1 MTR_4g021500 30.612 441 261 16 74 482 37 464 1.59e-51 188
MS.gene25854.t1 MTR_4g021500 29.782 413 255 15 89 483 487 882 9.39e-44 166
MS.gene25854.t1 MTR_4g021510 31.422 436 260 16 76 482 38 463 4.37e-51 181
MS.gene25854.t1 MTR_2g018600 29.252 441 268 14 77 483 34 464 1.89e-50 179
MS.gene25854.t1 MTR_4g057570 31.323 431 240 14 88 487 86 491 6.28e-50 178
MS.gene25854.t1 MTR_7g051430 37.282 287 168 4 58 339 16 295 4.19e-49 174
MS.gene25854.t1 MTR_4g057600 29.717 424 252 12 88 487 92 493 4.95e-49 176
MS.gene25854.t1 MTR_5g097180 30.529 416 251 12 93 487 43 441 5.59e-49 174
MS.gene25854.t1 MTR_3g100420 30.973 452 261 17 73 483 50 491 2.98e-48 174
MS.gene25854.t1 MTR_2g018630 29.930 431 271 13 77 483 37 460 1.02e-46 169
MS.gene25854.t1 MTR_4g021520 30.273 403 241 13 74 447 37 428 7.52e-44 160
MS.gene25854.t1 MTR_3g045770 27.778 432 286 12 77 487 27 453 7.58e-44 160
MS.gene25854.t1 MTR_2g009200 30.856 444 241 15 74 484 25 435 5.04e-42 155
MS.gene25854.t1 MTR_2g095710 29.177 401 241 13 106 487 106 482 1.59e-40 152
MS.gene25854.t1 MTR_4g084710 30.282 426 254 16 93 494 45 451 5.17e-39 147
MS.gene25854.t1 MTR_0075s0010 28.809 361 183 11 75 422 6 305 4.28e-37 139
MS.gene25854.t1 MTR_4g021535 26.364 440 236 18 74 483 33 414 1.93e-34 134
MS.gene25854.t1 MTR_2g018630 27.586 348 227 11 155 483 14 355 2.55e-28 115
MS.gene25854.t1 MTR_4g056520 27.294 436 253 19 94 483 36 453 2.49e-25 108
MS.gene25854.t1 MTR_1g051175 50.575 87 40 2 83 168 18 102 1.21e-21 95.1
MS.gene25854.t1 MTR_7g100560 29.493 217 86 6 87 303 68 217 2.35e-18 86.7
MS.gene25854.t1 MTR_2g084530 25.339 442 248 15 94 481 36 449 9.11e-17 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25854.t1 AT3G63470 52.966 472 200 8 25 483 29 491 2.16e-174 500
MS.gene25854.t1 AT4G30610 52.055 438 191 5 72 491 28 464 1.15e-156 454
MS.gene25854.t1 AT2G24010 51.981 429 194 6 73 491 27 453 1.61e-154 447
MS.gene25854.t1 AT3G07990 52.133 422 187 8 75 483 32 451 2.38e-150 437
MS.gene25854.t1 AT2G24000 49.885 435 196 6 72 486 26 458 5.23e-150 437
MS.gene25854.t1 AT2G24010 50.000 444 195 7 73 491 27 468 2.96e-149 435
MS.gene25854.t1 AT2G35780 51.174 426 189 7 72 483 25 445 4.88e-148 431
MS.gene25854.t1 AT5G23210 47.609 460 194 8 72 492 47 498 6.93e-147 430
MS.gene25854.t1 AT2G24010 47.527 465 192 8 77 491 2 464 5.08e-143 419
MS.gene25854.t1 AT2G35770 48.884 448 219 6 54 493 17 462 1.29e-142 417
MS.gene25854.t1 AT4G30810 47.166 441 218 5 68 494 27 466 2.66e-142 417
MS.gene25854.t1 AT3G02110 48.322 447 204 6 69 491 30 473 1.70e-141 415
MS.gene25854.t1 AT1G61130 49.425 435 200 8 75 491 30 462 7.52e-140 410
MS.gene25854.t1 AT1G11080 46.374 455 214 8 66 491 38 491 1.99e-138 408
MS.gene25854.t1 AT1G11080 46.374 455 214 8 66 491 38 491 4.13e-138 407
MS.gene25854.t1 AT3G52000 48.946 427 201 10 75 491 62 481 7.10e-133 394
MS.gene25854.t1 AT4G15100 46.742 445 208 9 73 491 41 482 1.19e-132 393
MS.gene25854.t1 AT3G52020 44.752 505 251 15 5 492 8 501 1.42e-132 393
MS.gene25854.t1 AT5G08260 46.485 441 206 10 70 483 33 470 2.32e-132 392
MS.gene25854.t1 AT2G05850 46.207 435 216 9 71 492 58 487 4.40e-131 389
MS.gene25854.t1 AT3G17180 45.861 447 214 9 72 492 34 478 7.66e-130 385
MS.gene25854.t1 AT5G23210 46.973 413 172 8 72 445 47 451 2.50e-128 381
MS.gene25854.t1 AT3G52010 45.413 436 211 12 73 491 61 486 5.53e-123 368
MS.gene25854.t1 AT1G43780 45.089 448 219 11 69 492 30 474 9.90e-123 367
MS.gene25854.t1 AT5G23210 45.854 410 176 7 122 492 1 403 1.89e-122 364
MS.gene25854.t1 AT5G23210 46.020 402 171 7 130 492 8 402 3.81e-121 360
MS.gene25854.t1 AT2G12480 45.125 441 221 10 69 492 24 460 8.49e-119 357
MS.gene25854.t1 AT2G33530 44.444 441 217 11 71 489 28 462 1.76e-114 346
MS.gene25854.t1 AT5G42240 43.371 445 227 9 69 491 25 466 1.13e-113 344
MS.gene25854.t1 AT1G28110 43.649 433 218 10 73 485 28 454 9.34e-113 341
MS.gene25854.t1 AT1G28110 43.649 433 218 10 73 485 28 454 9.34e-113 341
MS.gene25854.t1 AT2G12480 43.218 435 214 9 69 492 24 436 1.03e-111 338
MS.gene25854.t1 AT2G24010 48.459 357 157 7 77 408 2 356 7.95e-111 333
MS.gene25854.t1 AT2G12480 42.989 435 213 9 69 492 24 434 1.24e-110 335
MS.gene25854.t1 AT3G17180 43.781 402 199 8 72 448 34 433 9.59e-110 333
MS.gene25854.t1 AT5G42230 42.342 444 231 9 69 490 21 461 1.12e-109 333
MS.gene25854.t1 AT5G42230 42.342 444 231 9 69 490 21 461 1.12e-109 333
MS.gene25854.t1 AT2G12480 42.922 438 210 10 69 492 24 435 5.59e-109 331
MS.gene25854.t1 AT5G23210 45.070 355 149 7 130 445 8 355 2.12e-102 311
MS.gene25854.t1 AT4G12910 36.211 475 241 10 66 486 29 495 4.89e-83 266
MS.gene25854.t1 AT3G56540 65.027 183 58 3 53 231 52 232 9.20e-77 242
MS.gene25854.t1 AT3G25420 33.881 487 254 12 57 485 15 491 1.73e-76 248
MS.gene25854.t1 AT2G24000 43.165 278 138 4 228 486 119 395 5.25e-71 231
MS.gene25854.t1 AT2G24000 59.140 93 36 2 72 163 26 117 4.85e-28 115
MS.gene25854.t1 AT4G12910 34.713 435 223 9 66 447 29 455 6.44e-69 228
MS.gene25854.t1 AT2G22990 32.251 431 255 13 74 486 20 431 1.76e-60 205
MS.gene25854.t1 AT3G10410 32.019 431 244 13 82 487 90 496 8.62e-58 200
MS.gene25854.t1 AT3G45010 30.892 437 243 13 82 487 92 500 8.79e-57 197
MS.gene25854.t1 AT2G22990 32.227 422 250 12 74 477 20 423 1.21e-56 195
MS.gene25854.t1 AT2G23000 33.645 428 255 14 74 486 22 435 1.46e-56 194
MS.gene25854.t1 AT2G23000 33.645 428 255 14 74 486 22 435 1.46e-56 194
MS.gene25854.t1 AT2G22990 32.678 407 238 12 74 462 20 408 1.88e-56 194
MS.gene25854.t1 AT3G10450 31.702 429 258 11 74 486 26 435 1.58e-54 189
MS.gene25854.t1 AT5G22980 28.931 477 274 16 35 487 60 495 1.74e-54 191
MS.gene25854.t1 AT2G22970 32.779 421 256 10 77 485 25 430 1.89e-53 186
MS.gene25854.t1 AT2G22990 30.858 431 252 14 74 486 20 422 1.39e-52 184
MS.gene25854.t1 AT2G23010 31.368 424 262 11 77 485 25 434 2.39e-52 183
MS.gene25854.t1 AT1G73300 31.163 430 259 11 74 486 30 439 4.91e-52 182
MS.gene25854.t1 AT1G73300 31.163 430 259 11 74 486 30 439 4.91e-52 182
MS.gene25854.t1 AT2G22920 32.477 428 250 13 77 485 25 432 9.46e-52 182
MS.gene25854.t1 AT1G33540 31.707 451 266 14 68 485 20 461 1.05e-51 182
MS.gene25854.t1 AT2G23010 31.132 424 263 11 77 485 25 434 1.15e-51 181
MS.gene25854.t1 AT3G10450 31.618 408 244 11 95 486 4 392 1.60e-51 180
MS.gene25854.t1 AT1G73270 30.575 435 267 12 68 486 24 439 2.88e-51 181
MS.gene25854.t1 AT1G73270 30.575 435 267 12 68 486 24 439 3.61e-51 181
MS.gene25854.t1 AT5G36180 31.395 430 258 11 74 486 30 439 1.43e-50 179
MS.gene25854.t1 AT1G73280 31.507 438 259 12 68 486 24 439 2.42e-50 178
MS.gene25854.t1 AT2G22970 33.250 400 241 9 77 464 25 410 7.62e-50 177
MS.gene25854.t1 AT2G22980 31.265 419 257 11 77 483 26 425 9.48e-50 176
MS.gene25854.t1 AT3G12220 31.043 422 266 10 77 487 27 434 1.53e-49 176
MS.gene25854.t1 AT2G22970 33.083 399 241 9 77 463 25 409 2.00e-49 175
MS.gene25854.t1 AT1G33540 31.840 424 247 14 95 485 4 418 2.28e-49 175
MS.gene25854.t1 AT3G10450 31.527 406 244 10 74 463 26 413 4.92e-49 174
MS.gene25854.t1 AT3G12203 30.238 420 267 11 77 485 9 413 4.34e-48 171
MS.gene25854.t1 AT1G73290 30.787 432 268 13 68 486 23 436 6.11e-48 171
MS.gene25854.t1 AT1G73310 30.626 431 260 11 74 486 30 439 6.24e-48 171
MS.gene25854.t1 AT3G12203 30.166 421 268 11 77 486 30 435 6.84e-48 171
MS.gene25854.t1 AT5G09640 30.022 453 256 16 77 485 27 462 2.15e-47 171
MS.gene25854.t1 AT3G12230 30.332 422 265 13 77 485 27 432 3.43e-47 169
MS.gene25854.t1 AT2G22920 32.653 392 227 11 77 450 25 397 8.28e-47 167
MS.gene25854.t1 AT3G12220 30.693 404 255 10 95 487 15 404 1.05e-46 167
MS.gene25854.t1 AT2G22980 31.579 399 243 10 77 463 26 406 1.15e-46 167
MS.gene25854.t1 AT2G22980 31.658 398 242 10 77 462 26 405 1.68e-46 167
MS.gene25854.t1 AT3G12240 30.476 420 267 14 77 485 28 433 2.34e-46 167
MS.gene25854.t1 AT3G10450 31.429 385 230 10 95 463 4 370 7.86e-46 164
MS.gene25854.t1 AT3G12203 30.179 391 247 11 106 485 1 376 4.18e-44 160
MS.gene25854.t1 AT1G15000 30.073 409 248 15 93 485 37 423 7.14e-44 160
MS.gene25854.t1 AT1G73290 30.633 395 245 11 68 450 23 400 1.72e-43 159
MS.gene25854.t1 AT1G73310 30.789 393 235 9 74 449 30 402 7.11e-43 157
MS.gene25854.t1 AT3G12220 30.216 417 224 11 77 487 27 382 4.00e-42 154
MS.gene25854.t1 AT1G73290 35.065 231 143 3 68 293 23 251 4.16e-38 141
MS.gene25854.t1 AT2G22970 35.849 265 158 6 77 333 25 285 4.59e-38 142
MS.gene25854.t1 AT2G22920 35.338 266 159 5 77 333 25 286 5.50e-38 141
MS.gene25854.t1 AT2G22990 30.448 335 202 11 165 486 1 317 1.88e-37 140
MS.gene25854.t1 AT2G22990 30.149 335 203 11 165 486 1 317 7.58e-37 138
MS.gene25854.t1 AT1G73280 34.545 275 167 5 68 333 24 294 1.08e-36 139
MS.gene25854.t1 AT2G22980 35.714 266 155 6 77 333 26 284 5.12e-36 137
MS.gene25854.t1 AT5G36180 29.360 344 212 9 154 485 12 336 7.42e-31 122
MS.gene25854.t1 AT5G36180 29.360 344 212 9 154 485 12 336 7.42e-31 122
MS.gene25854.t1 AT1G73280 29.107 347 211 10 154 486 12 337 4.40e-30 120
MS.gene25854.t1 AT5G22960 35.795 176 91 4 82 257 25 178 1.96e-27 108
MS.gene25854.t1 AT1G73280 29.091 330 205 10 168 486 1 312 2.33e-27 112
MS.gene25854.t1 AT5G36180 28.571 322 200 8 154 463 12 315 5.14e-26 108
MS.gene25854.t1 AT2G22980 28.758 306 194 8 165 463 1 289 1.36e-24 103
MS.gene25854.t1 AT1G73310 28.571 308 189 7 154 449 12 300 3.14e-24 103
MS.gene25854.t1 AT5G36180 28.664 307 190 7 154 449 12 300 9.56e-24 102
MS.gene25854.t1 AT2G27920 26.376 436 257 18 94 482 37 455 1.15e-22 100
MS.gene25854.t1 AT2G22920 27.881 269 162 10 231 485 3 253 7.36e-13 68.9

Find 117 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTAGTATTGTGGCTTAA+TGG 0.193981 3.2:-69967630 MS.gene25854:CDS
ATTACTCAACTTGAGTTTAT+TGG 0.209420 3.2:-69967961 MS.gene25854:CDS
GCTTGACTAGCTTTGCTATA+TGG 0.232639 3.2:+69967930 None:intergenic
GACCCTTGTTCTGATTATTA+TGG 0.237969 3.2:-69966278 MS.gene25854:CDS
ACTGGGGTATGGAGCCTTTC+AGG 0.238241 3.2:-69967101 MS.gene25854:CDS
TGATGATGCCACAAGTTTAA+AGG 0.260603 3.2:-69966531 MS.gene25854:CDS
ACAGTTGGTAAGATGGTAAT+TGG 0.266699 3.2:+69966038 None:intergenic
GGTTTAGTTGAAGGGTTATA+TGG 0.270576 3.2:+69967651 None:intergenic
GGACGCCAAGCTGCGTTTAT+AGG 0.274588 3.2:+69965755 None:intergenic
CTTAGAACCAATTAATTTGA+TGG 0.278306 3.2:+69965346 None:intergenic
CTGCAATGGGTCCAATTTGC+AGG 0.302812 3.2:+69966206 None:intergenic
TCGATAGCGGCATTAAATTA+TGG 0.305820 3.2:-69966004 MS.gene25854:CDS
CTGCGTTTATAGGGAGCTTA+AGG 0.310250 3.2:+69965765 None:intergenic
TTCATGCAAAACCTGCAAAT+TGG 0.315255 3.2:-69966217 MS.gene25854:CDS
TATACCGTAACCCGTATTCT+TGG 0.321788 3.2:-69967039 MS.gene25854:CDS
AGGACCTGGCTGTTCCTCAC+TGG 0.332819 3.2:-69967119 MS.gene25854:CDS
GCTTGGCGTCCATGGTATTC+TGG 0.333942 3.2:-69965743 MS.gene25854:CDS
ATCCTCCCAAGACTCTTTCT+TGG 0.339640 3.2:-69967866 MS.gene25854:CDS
GATGATGCCACAAGTTTAAA+GGG 0.364178 3.2:-69966530 MS.gene25854:CDS
ATCATTGTCAAAGCCCGTTC+TGG 0.370318 3.2:+69965406 None:intergenic
ATGATCTCATCATTCCTCTA+TGG 0.384946 3.2:-69965388 MS.gene25854:CDS
GACGCCAAGCTGCGTTTATA+GGG 0.390982 3.2:+69965756 None:intergenic
TCCAAGCTGGCAACCAGAAC+GGG 0.395617 3.2:-69965419 MS.gene25854:CDS
TCACCTTCATTCCAAGAATA+CGG 0.395846 3.2:+69967028 None:intergenic
GCCCGTTCTGGTTGCCAGCT+TGG 0.397914 3.2:+69965418 None:intergenic
TTTATTGTTGTGAAGAATAG+TGG 0.404755 3.2:+69966702 None:intergenic
GGCTGTTCCTCACTGGGGTA+TGG 0.417363 3.2:-69967112 MS.gene25854:CDS
AACCTCTGGTCTATTTAGAT+AGG 0.422418 3.2:+69966250 None:intergenic
CTGCAAATTGGACCCATTGC+AGG 0.423562 3.2:-69966205 MS.gene25854:intron
AATTTCAGATAGGGAATGCT+TGG 0.423855 3.2:-69966556 MS.gene25854:intron
CAAGACTCTTTCTTGGGAAG+TGG 0.424295 3.2:-69967859 MS.gene25854:CDS
TATGAAAATCCCACTCCTGC+TGG 0.427452 3.2:+69966889 None:intergenic
TCCTCCCAAGACTCTTTCTT+GGG 0.428322 3.2:-69967865 MS.gene25854:CDS
GCATTAAATTATGGATATAC+AGG 0.431413 3.2:-69965995 MS.gene25854:intron
AACATACAGAAATGCCTTTG+AGG 0.436367 3.2:+69966656 None:intergenic
CCTATGTCTTCCTCATCAAC+TGG 0.443865 3.2:-69966810 MS.gene25854:CDS
TGCGTTTATAGGGAGCTTAA+GGG 0.446999 3.2:+69965766 None:intergenic
TTGCTCAAAATTCACTCCAT+AGG 0.448912 3.2:+69967740 None:intergenic
AATTAATTTGATGGTGAACT+AGG 0.449264 3.2:+69965355 None:intergenic
CACTTCCCAAGAAAGAGTCT+TGG 0.457536 3.2:+69967860 None:intergenic
AATACTAAAGGTTTAGTTGA+AGG 0.459170 3.2:+69967642 None:intergenic
GGACCTGGCTGTTCCTCACT+GGG 0.459617 3.2:-69967118 MS.gene25854:CDS
CATTCCAAGAATACGGGTTA+CGG 0.463929 3.2:+69967035 None:intergenic
CTAAAGGCCTTCTTTGAACA+TGG 0.467756 3.2:-69966389 MS.gene25854:CDS
CAACTAAACCTTTAGTATTG+TGG 0.468364 3.2:-69967638 MS.gene25854:CDS
ATCTCCTGATTTGTCATAGT+CGG 0.468625 3.2:+69966852 None:intergenic
ACTTCCCAAGAAAGAGTCTT+GGG 0.474421 3.2:+69967861 None:intergenic
GAGGAAGACATAGGCATCCT+TGG 0.475321 3.2:+69966819 None:intergenic
TATCTTTCCATGTTCAAAGA+AGG 0.476920 3.2:+69966382 None:intergenic
TGCTCAAAATTCACTCCATA+GGG 0.485269 3.2:+69967741 None:intergenic
ACATTGCCTGGTCAACCCTA+TGG 0.493936 3.2:-69967756 MS.gene25854:CDS
AAATAGACCAGAGGTTCAAA+AGG 0.494500 3.2:-69966243 MS.gene25854:CDS
ATACTAAAGGTTTAGTTGAA+GGG 0.494760 3.2:+69967643 None:intergenic
AAAAGCTCTCTTCCAGCCTC+AGG 0.499459 3.2:+69967693 None:intergenic
CACCTTCATTCCAAGAATAC+GGG 0.500312 3.2:+69967029 None:intergenic
TAAACGCAGCTTGGCGTCCA+TGG 0.502210 3.2:-69965751 MS.gene25854:CDS
TATAGAATATTACCTGCAAT+GGG 0.505247 3.2:+69966193 None:intergenic
TTATAGAATATTACCTGCAA+TGG 0.506360 3.2:+69966192 None:intergenic
TATCAATAATGTAACACTAA+AGG 0.509465 3.2:-69966405 MS.gene25854:CDS
CCATTAAGCCACAATACTAA+AGG 0.509726 3.2:+69967630 None:intergenic
GTCACTGTTGATCCTGAGGC+TGG 0.511770 3.2:-69967705 MS.gene25854:CDS
GCTGATGTTGCACCTCAAGA+GGG 0.512467 3.2:-69967804 MS.gene25854:CDS
ACATCCGACTATGACAAATC+AGG 0.514877 3.2:-69966856 MS.gene25854:CDS
TTTGTTACAGTGAGAGGAGC+AGG 0.515378 3.2:-69965451 MS.gene25854:CDS
TGTCTTCCTCATCAACTGGC+TGG 0.515449 3.2:-69966806 MS.gene25854:CDS
AACCCGTATTCTTGGAATGA+AGG 0.522806 3.2:-69967031 MS.gene25854:intron
GACAAGATTGACACATTGCC+TGG 0.523116 3.2:-69967768 MS.gene25854:CDS
CCATCAGAGTTGATTCTGAA+GGG 0.523486 3.2:+69967070 None:intergenic
TGCTGATGTTGCACCTCAAG+AGG 0.525644 3.2:-69967805 MS.gene25854:CDS
TCCATGGTATTCTGGAAAAG+AGG 0.526540 3.2:-69965735 MS.gene25854:intron
GCCATCAGAGTTGATTCTGA+AGG 0.530541 3.2:+69967069 None:intergenic
ATACTTGACAGTTGGTAAGA+TGG 0.530659 3.2:+69966031 None:intergenic
AGTTGAGAACAAGATGAAGA+AGG 0.533495 3.2:-69968003 None:intergenic
TTGACATTTGTTACAGTGAG+AGG 0.534371 3.2:-69965457 MS.gene25854:CDS
TTCACTCCATAGGGTTGACC+AGG 0.534868 3.2:+69967750 None:intergenic
CCACTCCTGCTGGAGACTCT+AGG 0.536283 3.2:+69966899 None:intergenic
ATGACCAGCATAACTCTCTC+CGG 0.536968 3.2:+69966744 None:intergenic
ATAGTCGTCTTCTTACTGAC+TGG 0.537929 3.2:-69966070 MS.gene25854:intron
CCTAGAGTCTCCAGCAGGAG+TGG 0.545565 3.2:-69966899 MS.gene25854:CDS
GGATATGTGGTGGGATACAA+AGG 0.546750 3.2:-69965481 MS.gene25854:CDS
TTCCAAGCTGGCAACCAGAA+CGG 0.550031 3.2:-69965420 MS.gene25854:CDS
AGCTCCCTATAAACGCAGCT+TGG 0.550821 3.2:-69965760 MS.gene25854:CDS
ATCTCTCCAGCCAGTTGATG+AGG 0.560761 3.2:+69966800 None:intergenic
AACACTGTTATTAACCTCAA+AGG 0.561939 3.2:-69966670 MS.gene25854:CDS
CAGGACATCTTGTTCCAAGC+TGG 0.567894 3.2:-69965432 MS.gene25854:CDS
CTAGAGTCTCCAGCAGGAGT+GGG 0.569156 3.2:-69966898 MS.gene25854:CDS
TACCCCAGTGAGGAACAGCC+AGG 0.578470 3.2:+69967115 None:intergenic
CCCTTCAGAATCAACTCTGA+TGG 0.578815 3.2:-69967070 MS.gene25854:CDS
ATAACCGGAGAGAGTTATGC+TGG 0.580139 3.2:-69966748 MS.gene25854:CDS
CTCTTCCTAGAGTCTCCAGC+AGG 0.582921 3.2:-69966904 MS.gene25854:CDS
TCGATGAGATACTTGACAGT+TGG 0.583309 3.2:+69966023 None:intergenic
TAATAATAATGACAGATTGG+AGG 0.585531 3.2:-69965502 MS.gene25854:intron
TATGGAGCCTTTCAGGAGCT+AGG 0.588840 3.2:-69967094 MS.gene25854:CDS
GAAGGGTCCTAGCTCCTGAA+AGG 0.589336 3.2:+69967087 None:intergenic
AGGAGATAAATCAACTGCCA+AGG 0.589939 3.2:-69966836 MS.gene25854:CDS
GCTTGCATTAGCCCCTCTTG+AGG 0.590255 3.2:+69967792 None:intergenic
CCAGTTGATGAGGAAGACAT+AGG 0.602150 3.2:+69966810 None:intergenic
TTAGTATTGTGGCTTAATGG+AGG 0.610025 3.2:-69967627 MS.gene25854:intron
TAGGAGGCAAACTTCCATAG+AGG 0.611627 3.2:+69965374 None:intergenic
CAGTGGTGATACAGATGCAG+TGG 0.613592 3.2:-69965822 MS.gene25854:CDS
ATGACAGATTGGAGGATATG+TGG 0.614174 3.2:-69965494 MS.gene25854:intron
GAACCATAATAATCAGAACA+AGG 0.615337 3.2:+69966275 None:intergenic
AGTTCAAAGCATGAGTCCAA+AGG 0.615853 3.2:+69966495 None:intergenic
CTACGTCACTGTTGATCCTG+AGG 0.618660 3.2:-69967709 MS.gene25854:CDS
TAATTTGATGGTGAACTAGG+AGG 0.626163 3.2:+69965358 None:intergenic
ATGATGCCACAAGTTTAAAG+GGG 0.629930 3.2:-69966529 MS.gene25854:CDS
GTCAAGTATCTCATCGATAG+CGG 0.632646 3.2:-69966017 MS.gene25854:CDS
CAGATTGGAGGATATGTGGT+GGG 0.633074 3.2:-69965490 MS.gene25854:CDS
AACCATAATAATCAGAACAA+GGG 0.642040 3.2:+69966276 None:intergenic
CTGATGTTGCACCTCAAGAG+GGG 0.658453 3.2:-69967803 MS.gene25854:CDS
TCCCAAGAAAGAGTCTTGGG+AGG 0.663424 3.2:+69967864 None:intergenic
AGAATAGTGGATGCAAGCTG+AGG 0.669219 3.2:+69966715 None:intergenic
TGCCTATCTAAATAGACCAG+AGG 0.674801 3.2:-69966252 MS.gene25854:CDS
AGAGAGGAGTCATGACACAA+TGG 0.676190 3.2:+69966338 None:intergenic
ACAGATTGGAGGATATGTGG+TGG 0.699486 3.2:-69965491 MS.gene25854:intron
AAAGGCTCCATACCCCAGTG+AGG 0.728637 3.2:+69967105 None:intergenic
GACCTGGCTGTTCCTCACTG+GGG 0.729552 3.2:-69967117 MS.gene25854:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCAAAAATGTTATTACT+TGG + chr3.2:69967813-69967832 None:intergenic 15.0%
!! CATATTTCATAATGATAAAT+TGG + chr3.2:69966409-69966428 None:intergenic 15.0%
!!! ACTTTAAAAAATTTCAGATA+GGG - chr3.2:69966782-69966801 MS.gene25854:CDS 15.0%
!!! TACTTTAAAAAATTTCAGAT+AGG - chr3.2:69966781-69966800 MS.gene25854:CDS 15.0%
!!! TTGTTATTTTGTAAATTTGA+GGG - chr3.2:69967708-69967727 MS.gene25854:CDS 15.0%
!!! TTTGTTATTTTGTAAATTTG+AGG - chr3.2:69967707-69967726 MS.gene25854:CDS 15.0%
!! TATCAATAATGTAACACTAA+AGG - chr3.2:69966942-69966961 MS.gene25854:intron 20.0%
!! TTAATTTATTCGTAGTTTGA+TGG - chr3.2:69967643-69967662 MS.gene25854:CDS 20.0%
!! TTGTAATAATAATGACAGAT+TGG - chr3.2:69967842-69967861 MS.gene25854:CDS 20.0%
!!! AGAGCTTTTTTATTATTTTG+TGG - chr3.2:69965668-69965687 MS.gene25854:intron 20.0%
!!! CTTTTGATATTAAAAGAACA+TGG - chr3.2:69966011-69966030 MS.gene25854:CDS 20.0%
!!! TTGCAAATTTTCTTATTTAG+AGG + chr3.2:69965883-69965902 None:intergenic 20.0%
! AACCATAATAATCAGAACAA+GGG + chr3.2:69967074-69967093 None:intergenic 25.0%
! AATACTAAAGGTTTAGTTGA+AGG + chr3.2:69965708-69965727 None:intergenic 25.0%
! ATACTAAAGGTTTAGTTGAA+GGG + chr3.2:69965707-69965726 None:intergenic 25.0%
! ATTACTCAACTTGAGTTTAT+TGG - chr3.2:69965386-69965405 MS.gene25854:CDS 25.0%
! ATTACTTGGTATACAAGAAA+AGG + chr3.2:69967799-69967818 None:intergenic 25.0%
! GCATTAAATTATGGATATAC+AGG - chr3.2:69967352-69967371 MS.gene25854:intron 25.0%
! TAATAATAATGACAGATTGG+AGG - chr3.2:69967845-69967864 MS.gene25854:CDS 25.0%
! TATAGAATATTACCTGCAAT+GGG + chr3.2:69967157-69967176 None:intergenic 25.0%
! TTATAGAATATTACCTGCAA+TGG + chr3.2:69967158-69967177 None:intergenic 25.0%
! TTTATTGTTGTGAAGAATAG+TGG + chr3.2:69966648-69966667 None:intergenic 25.0%
AAAAAACCCCTTTAAACTTG+TGG + chr3.2:69966827-69966846 None:intergenic 30.0%
AACACTGTTATTAACCTCAA+AGG - chr3.2:69966677-69966696 MS.gene25854:CDS 30.0%
ATTCTAATTCACTCCATATG+TGG - chr3.2:69967762-69967781 MS.gene25854:CDS 30.0%
CAACTAAACCTTTAGTATTG+TGG - chr3.2:69965709-69965728 MS.gene25854:intron 30.0%
GAACCATAATAATCAGAACA+AGG + chr3.2:69967075-69967094 None:intergenic 30.0%
GGTAATAAATCAACCACATA+TGG + chr3.2:69967778-69967797 None:intergenic 30.0%
TAAAAGAACATGGTAAAACC+TGG - chr3.2:69966021-69966040 MS.gene25854:CDS 30.0%
TATCTTTCCATGTTCAAAGA+AGG + chr3.2:69966968-69966987 None:intergenic 30.0%
TCCTCTTGATGAAATTATTC+CGG - chr3.2:69966071-69966090 MS.gene25854:intron 30.0%
TGTGAATAATGACTAATGAG+AGG - chr3.2:69967467-69967486 MS.gene25854:intron 30.0%
TGTGAGTTACATTTCATACT+TGG + chr3.2:69966731-69966750 None:intergenic 30.0%
TTCTATGCTAATCAAGAATG+TGG - chr3.2:69967236-69967255 MS.gene25854:intron 30.0%
! ACATAATGTTTGAAACTCAC+AGG - chr3.2:69966208-69966227 MS.gene25854:CDS 30.0%
! ACCAGAGATTTTTACATAAC+CGG - chr3.2:69966584-69966603 MS.gene25854:intron 30.0%
! GTGAATTTTGAGCAATATTC+AGG - chr3.2:69965615-69965634 MS.gene25854:intron 30.0%
! TCATAGTTGTAAGAGTGTAA+GGG + chr3.2:69966123-69966142 None:intergenic 30.0%
! TTCCAACTCCTAAATTTTGA+TGG - chr3.2:69967490-69967509 MS.gene25854:intron 30.0%
AAATAGACCAGAGGTTCAAA+AGG - chr3.2:69967104-69967123 MS.gene25854:CDS 35.0%
AACATACAGAAATGCCTTTG+AGG + chr3.2:69966694-69966713 None:intergenic 35.0%
AACCTCTGGTCTATTTAGAT+AGG + chr3.2:69967100-69967119 None:intergenic 35.0%
AATTTCAGATAGGGAATGCT+TGG - chr3.2:69966791-69966810 MS.gene25854:CDS 35.0%
AGAACGCACCATCAAAATTT+AGG + chr3.2:69967501-69967520 None:intergenic 35.0%
ATGATCTCATCATTCCTCTA+TGG - chr3.2:69967959-69967978 MS.gene25854:CDS 35.0%
ATGATGCCACAAGTTTAAAG+GGG - chr3.2:69966818-69966837 MS.gene25854:CDS 35.0%
CACCATCAAAATTTAGGAGT+TGG + chr3.2:69967495-69967514 None:intergenic 35.0%
CCATTAAGCCACAATACTAA+AGG + chr3.2:69965720-69965739 None:intergenic 35.0%
CCTTTAGTATTGTGGCTTAA+TGG - chr3.2:69965717-69965736 MS.gene25854:intron 35.0%
GACCCTTGTTCTGATTATTA+TGG - chr3.2:69967069-69967088 MS.gene25854:CDS 35.0%
GATGATGCCACAAGTTTAAA+GGG - chr3.2:69966817-69966836 MS.gene25854:CDS 35.0%
GGTTTAGTTGAAGGGTTATA+TGG + chr3.2:69965699-69965718 None:intergenic 35.0%
TCACCTTCATTCCAAGAATA+CGG + chr3.2:69966322-69966341 None:intergenic 35.0%
TCCGGTTATGTAAAAATCTC+TGG + chr3.2:69966588-69966607 None:intergenic 35.0%
TGCTCAAAATTCACTCCATA+GGG + chr3.2:69965609-69965628 None:intergenic 35.0%
TTAGTATTGTGGCTTAATGG+AGG - chr3.2:69965720-69965739 MS.gene25854:intron 35.0%
TTCATGCAAAACCTGCAAAT+TGG - chr3.2:69967130-69967149 MS.gene25854:intron 35.0%
TTGCTCAAAATTCACTCCAT+AGG + chr3.2:69965610-69965629 None:intergenic 35.0%
! ATCTCCTGATTTGTCATAGT+CGG + chr3.2:69966498-69966517 None:intergenic 35.0%
! CTCATAGTTGTAAGAGTGTA+AGG + chr3.2:69966124-69966143 None:intergenic 35.0%
! TCGATAGCGGCATTAAATTA+TGG - chr3.2:69967343-69967362 MS.gene25854:intron 35.0%
! TCTCTGAAAAATGGTTCAAC+TGG - chr3.2:69967027-69967046 MS.gene25854:intron 35.0%
! TGATGATGCCACAAGTTTAA+AGG - chr3.2:69966816-69966835 MS.gene25854:CDS 35.0%
! TGATGATGCTCATAATAACC+AGG + chr3.2:69966042-69966061 None:intergenic 35.0%
! TTGACATTTGTTACAGTGAG+AGG - chr3.2:69967890-69967909 MS.gene25854:CDS 35.0%
!! ACAGTTGGTAAGATGGTAAT+TGG + chr3.2:69967312-69967331 None:intergenic 35.0%
!! AGTTGAACCATTTTTCAGAG+AGG + chr3.2:69967028-69967047 None:intergenic 35.0%
!! ATACTTGACAGTTGGTAAGA+TGG + chr3.2:69967319-69967338 None:intergenic 35.0%
!! TAATTTGATGGTGAACTAGG+AGG + chr3.2:69967992-69968011 None:intergenic 35.0%
!!! AAATCTCTGGTTTTGTACTG+TGG + chr3.2:69966575-69966594 None:intergenic 35.0%
!!! AGGGGTTTTTTGATTACCTT+TGG - chr3.2:69966836-69966855 MS.gene25854:CDS 35.0%
AACCCGTATTCTTGGAATGA+AGG - chr3.2:69966316-69966335 MS.gene25854:CDS 40.0%
ACATCCGACTATGACAAATC+AGG - chr3.2:69966491-69966510 MS.gene25854:CDS 40.0%
ACTTCCCAAGAAAGAGTCTT+GGG + chr3.2:69965489-69965508 None:intergenic 40.0%
AGGAGATAAATCAACTGCCA+AGG - chr3.2:69966511-69966530 MS.gene25854:CDS 40.0%
AGTTCAAAGCATGAGTCCAA+AGG + chr3.2:69966855-69966874 None:intergenic 40.0%
ATAGTCGTCTTCTTACTGAC+TGG - chr3.2:69967277-69967296 MS.gene25854:intron 40.0%
ATGACAGATTGGAGGATATG+TGG - chr3.2:69967853-69967872 MS.gene25854:CDS 40.0%
CACCTTCATTCCAAGAATAC+GGG + chr3.2:69966321-69966340 None:intergenic 40.0%
CTAAAGGCCTTCTTTGAACA+TGG - chr3.2:69966958-69966977 MS.gene25854:intron 40.0%
CTTATTTAGAGGTCTCTGCA+AGG + chr3.2:69965872-69965891 None:intergenic 40.0%
GCCGGAATAATTTCATCAAG+AGG + chr3.2:69966075-69966094 None:intergenic 40.0%
GTCAAGTATCTCATCGATAG+CGG - chr3.2:69967330-69967349 MS.gene25854:intron 40.0%
TATACCGTAACCCGTATTCT+TGG - chr3.2:69966308-69966327 MS.gene25854:CDS 40.0%
TCGATGAGATACTTGACAGT+TGG + chr3.2:69967327-69967346 None:intergenic 40.0%
TGATGAAATTATTCCGGCCA+TGG - chr3.2:69966077-69966096 MS.gene25854:intron 40.0%
TGCCTATCTAAATAGACCAG+AGG - chr3.2:69967095-69967114 MS.gene25854:CDS 40.0%
TGCGTTTATAGGGAGCTTAA+GGG + chr3.2:69967584-69967603 None:intergenic 40.0%
! ACCTCTTTTCCAGAATACCA+TGG + chr3.2:69967616-69967635 None:intergenic 40.0%
! CATGACTCCTCTCTGAAAAA+TGG - chr3.2:69967018-69967037 MS.gene25854:intron 40.0%
! CATTCCAAGAATACGGGTTA+CGG + chr3.2:69966315-69966334 None:intergenic 40.0%
! CCATCAGAGTTGATTCTGAA+GGG + chr3.2:69966280-69966299 None:intergenic 40.0%
! TCCATGGTATTCTGGAAAAG+AGG - chr3.2:69967612-69967631 MS.gene25854:intron 40.0%
!! GCTTGACTAGCTTTGCTATA+TGG + chr3.2:69965420-69965439 None:intergenic 40.0%
ACAGATTGGAGGATATGTGG+TGG - chr3.2:69967856-69967875 MS.gene25854:CDS 45.0%
AGAGAGGAGTCATGACACAA+TGG + chr3.2:69967012-69967031 None:intergenic 45.0%
ATAACCGGAGAGAGTTATGC+TGG - chr3.2:69966599-69966618 MS.gene25854:intron 45.0%
ATCATTGTCAAAGCCCGTTC+TGG + chr3.2:69967944-69967963 None:intergenic 45.0%
ATCCTCCCAAGACTCTTTCT+TGG - chr3.2:69965481-69965500 MS.gene25854:CDS 45.0%
ATGACCAGCATAACTCTCTC+CGG + chr3.2:69966606-69966625 None:intergenic 45.0%
CAAGACTCTTTCTTGGGAAG+TGG - chr3.2:69965488-69965507 MS.gene25854:CDS 45.0%
CACTTCCCAAGAAAGAGTCT+TGG + chr3.2:69965490-69965509 None:intergenic 45.0%
CAGATTGGAGGATATGTGGT+GGG - chr3.2:69967857-69967876 MS.gene25854:CDS 45.0%
CCCTTCAGAATCAACTCTGA+TGG - chr3.2:69966277-69966296 MS.gene25854:CDS 45.0%
CCTATGTCTTCCTCATCAAC+TGG - chr3.2:69966537-69966556 MS.gene25854:CDS 45.0%
CTGCGTTTATAGGGAGCTTA+AGG + chr3.2:69967585-69967604 None:intergenic 45.0%
GACAAGATTGACACATTGCC+TGG - chr3.2:69965579-69965598 MS.gene25854:intron 45.0%
GAGAGTTTAACAACCATGGC+CGG + chr3.2:69966093-69966112 None:intergenic 45.0%
GCCATGGTTGTTAAACTCTC+GGG - chr3.2:69966093-69966112 MS.gene25854:intron 45.0%
GGCCATGGTTGTTAAACTCT+CGG - chr3.2:69966092-69966111 MS.gene25854:intron 45.0%
TAGGAGGCAAACTTCCATAG+AGG + chr3.2:69967976-69967995 None:intergenic 45.0%
TATGAAAATCCCACTCCTGC+TGG + chr3.2:69966461-69966480 None:intergenic 45.0%
TCCTCCCAAGACTCTTTCTT+GGG - chr3.2:69965482-69965501 MS.gene25854:CDS 45.0%
TGCATCTGTATCACCACTGT+AGG + chr3.2:69967524-69967543 None:intergenic 45.0%
TTTGTTACAGTGAGAGGAGC+AGG - chr3.2:69967896-69967915 MS.gene25854:CDS 45.0%
! GCCATCAGAGTTGATTCTGA+AGG + chr3.2:69966281-69966300 None:intergenic 45.0%
! GGATATGTGGTGGGATACAA+AGG - chr3.2:69967866-69967885 MS.gene25854:CDS 45.0%
! TGAAGAGCCTTTTGAACCTC+TGG + chr3.2:69967114-69967133 None:intergenic 45.0%
! TGTTTGAAACTCACAGGACC+TGG - chr3.2:69966214-69966233 MS.gene25854:CDS 45.0%
!! AGAATAGTGGATGCAAGCTG+AGG + chr3.2:69966635-69966654 None:intergenic 45.0%
!! CCAGTTGATGAGGAAGACAT+AGG + chr3.2:69966540-69966559 None:intergenic 45.0%
AAAAGCTCTCTTCCAGCCTC+AGG + chr3.2:69965657-69965676 None:intergenic 50.0%
ACATTGCCTGGTCAACCCTA+TGG - chr3.2:69965591-69965610 MS.gene25854:intron 50.0%
AGCTCCCTATAAACGCAGCT+TGG - chr3.2:69967587-69967606 MS.gene25854:intron 50.0%
CAGGACATCTTGTTCCAAGC+TGG - chr3.2:69967915-69967934 MS.gene25854:CDS 50.0%
CAGTGGTGATACAGATGCAG+TGG - chr3.2:69967525-69967544 MS.gene25854:intron 50.0%
CTACGTCACTGTTGATCCTG+AGG - chr3.2:69965638-69965657 MS.gene25854:intron 50.0%
CTGATGTTGCACCTCAAGAG+GGG - chr3.2:69965544-69965563 MS.gene25854:intron 50.0%
CTGCAAATTGGACCCATTGC+AGG - chr3.2:69967142-69967161 MS.gene25854:intron 50.0%
CTGCAATGGGTCCAATTTGC+AGG + chr3.2:69967144-69967163 None:intergenic 50.0%
GACGCCAAGCTGCGTTTATA+GGG + chr3.2:69967594-69967613 None:intergenic 50.0%
GAGGAAGACATAGGCATCCT+TGG + chr3.2:69966531-69966550 None:intergenic 50.0%
GCCCGAGAGTTTAACAACCA+TGG + chr3.2:69966097-69966116 None:intergenic 50.0%
GCTGATGTTGCACCTCAAGA+GGG - chr3.2:69965543-69965562 MS.gene25854:intron 50.0%
TATGGAGCCTTTCAGGAGCT+AGG - chr3.2:69966253-69966272 MS.gene25854:CDS 50.0%
TCCCAAGAAAGAGTCTTGGG+AGG + chr3.2:69965486-69965505 None:intergenic 50.0%
TGCTGATGTTGCACCTCAAG+AGG - chr3.2:69965542-69965561 MS.gene25854:intron 50.0%
TGTCTTCCTCATCAACTGGC+TGG - chr3.2:69966541-69966560 MS.gene25854:CDS 50.0%
TTCACTCCATAGGGTTGACC+AGG + chr3.2:69965600-69965619 None:intergenic 50.0%
TTCCAAGCTGGCAACCAGAA+CGG - chr3.2:69967927-69967946 MS.gene25854:CDS 50.0%
! ATCTCTCCAGCCAGTTGATG+AGG + chr3.2:69966550-69966569 None:intergenic 50.0%
AAAGGCTCCATACCCCAGTG+AGG + chr3.2:69966245-69966264 None:intergenic 55.0%
ACTGGGGTATGGAGCCTTTC+AGG - chr3.2:69966246-69966265 MS.gene25854:CDS 55.0%
CTAGAGTCTCCAGCAGGAGT+GGG - chr3.2:69966449-69966468 MS.gene25854:CDS 55.0%
CTCTTCCTAGAGTCTCCAGC+AGG - chr3.2:69966443-69966462 MS.gene25854:CDS 55.0%
GAAGGGTCCTAGCTCCTGAA+AGG + chr3.2:69966263-69966282 None:intergenic 55.0%
GATGGTGCGTTCTCCTACAG+TGG - chr3.2:69967508-69967527 MS.gene25854:intron 55.0%
GCTTGCATTAGCCCCTCTTG+AGG + chr3.2:69965558-69965577 None:intergenic 55.0%
GCTTGGCGTCCATGGTATTC+TGG - chr3.2:69967604-69967623 MS.gene25854:intron 55.0%
GGACGCCAAGCTGCGTTTAT+AGG + chr3.2:69967595-69967614 None:intergenic 55.0%
GTCACTGTTGATCCTGAGGC+TGG - chr3.2:69965642-69965661 MS.gene25854:intron 55.0%
TAAACGCAGCTTGGCGTCCA+TGG - chr3.2:69967596-69967615 MS.gene25854:intron 55.0%
TCCAAGCTGGCAACCAGAAC+GGG - chr3.2:69967928-69967947 MS.gene25854:CDS 55.0%
AGGACCTGGCTGTTCCTCAC+TGG - chr3.2:69966228-69966247 MS.gene25854:CDS 60.0%
CCACTCCTGCTGGAGACTCT+AGG + chr3.2:69966451-69966470 None:intergenic 60.0%
CCTAGAGTCTCCAGCAGGAG+TGG - chr3.2:69966448-69966467 MS.gene25854:CDS 60.0%
GACCTGGCTGTTCCTCACTG+GGG - chr3.2:69966230-69966249 MS.gene25854:CDS 60.0%
GGACCTGGCTGTTCCTCACT+GGG - chr3.2:69966229-69966248 MS.gene25854:CDS 60.0%
GGCTGTTCCTCACTGGGGTA+TGG - chr3.2:69966235-69966254 MS.gene25854:CDS 60.0%
TACCCCAGTGAGGAACAGCC+AGG + chr3.2:69966235-69966254 None:intergenic 60.0%
! GCCCGTTCTGGTTGCCAGCT+TGG + chr3.2:69967932-69967951 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69965357 69968012 69965357 ID=MS.gene25854
chr3.2 mRNA 69965357 69968012 69965357 ID=MS.gene25854.t1;Parent=MS.gene25854
chr3.2 exon 69967628 69968012 69967628 ID=MS.gene25854.t1.exon1;Parent=MS.gene25854.t1
chr3.2 CDS 69967628 69968012 69967628 ID=cds.MS.gene25854.t1;Parent=MS.gene25854.t1
chr3.2 exon 69967032 69967139 69967032 ID=MS.gene25854.t1.exon2;Parent=MS.gene25854.t1
chr3.2 CDS 69967032 69967139 69967032 ID=cds.MS.gene25854.t1;Parent=MS.gene25854.t1
chr3.2 exon 69966663 69966937 69966663 ID=MS.gene25854.t1.exon3;Parent=MS.gene25854.t1
chr3.2 CDS 69966663 69966937 69966663 ID=cds.MS.gene25854.t1;Parent=MS.gene25854.t1
chr3.2 exon 69966206 69966570 69966206 ID=MS.gene25854.t1.exon4;Parent=MS.gene25854.t1
chr3.2 CDS 69966206 69966570 69966206 ID=cds.MS.gene25854.t1;Parent=MS.gene25854.t1
chr3.2 exon 69965996 69966088 69965996 ID=MS.gene25854.t1.exon5;Parent=MS.gene25854.t1
chr3.2 CDS 69965996 69966088 69965996 ID=cds.MS.gene25854.t1;Parent=MS.gene25854.t1
chr3.2 exon 69965736 69965841 69965736 ID=MS.gene25854.t1.exon6;Parent=MS.gene25854.t1
chr3.2 CDS 69965736 69965841 69965736 ID=cds.MS.gene25854.t1;Parent=MS.gene25854.t1
chr3.2 exon 69965357 69965509 69965357 ID=MS.gene25854.t1.exon7;Parent=MS.gene25854.t1
chr3.2 CDS 69965357 69965509 69965357 ID=cds.MS.gene25854.t1;Parent=MS.gene25854.t1
Gene Sequence

>MS.gene25854

ATGAAGAAGGTTTCTCTTTATGCTTGTTTATTACTCAACTTGAGTTTATTGGTTATTTTTCCATATAGCAAAGCTAGTCAAGCTGATAAACTCAATGAGTTTATTCTGTCTAGAAAATCTCAGAATCCTCCCAAGACTCTTTCTTGGGAAGTGGAAGATGCAGTGAAAACACTTTCTTCTTCTGCTGCTGATGTTGCACCTCAAGAGGGGCTAATGCAAGCTGACAAGATTGACACATTGCCTGGTCAACCCTATGGAGTGAATTTTGAGCAATATTCAGGCTACGTCACTGTTGATCCTGAGGCTGGAAGAGAGCTTTTTTATTATTTTGTGGAGTCTCCATATAACCCTTCAACTAAACCTTTAGTATTGTGGCTTAATGGAGGTAAAAATTGCATTAGAAACTATTTTTCACAGTTAACTTTTGATCTTTTGTATGCATTTTCTATTTTTTATGTTCTTTAATGTGTCATTGTCTATTTATATTATGATGTTTGAAATTGTGAAAATTACCTTGCAGAGACCTCTAAATAAGAAAATTTGCAACACTGCACAAGATAAAAATAGTTTATAAATAGATTATTAATTATACTTGATTGATGATATAACTTCATTTTCATAGTAGTGAAACATGATAGTATAGTTTTAATTTTTCTTTTGATATTAAAAGAACATGGTAAAACCTGGTTATTATGAGCATCATCATCTGAATTTTCCTCTTGATGAAATTATTCCGGCCATGGTTGTTAAACTCTCGGGCTAACCCTTACACTCTTACAACTATGAGTCGAGAGTCTATGTAAGCTCAACAAGTTTACTTAGTCGAGATTATGACAGCTTTGATTCTGACAACATAATGTTTGAAACTCACAGGACCTGGCTGTTCCTCACTGGGGTATGGAGCCTTTCAGGAGCTAGGACCCTTCAGAATCAACTCTGATGGCAAAACACTATACCGTAACCCGTATTCTTGGAATGAAGGTGAGACAATCATCAATAGAACGAAATTGTTTCTTATGATGCATTTATTCTGAGACATGAATATTTTACCAATTTATCATTATGAAATATGCAGTTGCAAATATACTCTTCCTAGAGTCTCCAGCAGGAGTGGGATTTTCATACTCAAATACAACATCCGACTATGACAAATCAGGAGATAAATCAACTGCCAAGGATGCCTATGTCTTCCTCATCAACTGGCTGGAGAGATTTCCACAGTACAAAACCAGAGATTTTTACATAACCGGAGAGAGTTATGCTGGTCATTATGTTCCTCAGCTTGCATCCACTATTCTTCACAACAATAAACTCTATAACAACACTGTTATTAACCTCAAAGGCATTTCTGTATGTTCAAACTTCAAAAGACCAAGTATGAAATGTAACTCACAATCTCATAAATCTAACTTCATTATTATCTTTACTTTAAAAAATTTCAGATAGGGAATGCTTGGATTGATGATGCCACAAGTTTAAAGGGGTTTTTTGATTACCTTTGGACTCATGCTTTGAACTCAGATCAAACTCATGAGTTGATTGAGAAGTACTGTGACTTCACTACTGAAAATGTTTCAGCAATTTGTATCAATAATGTAACACTAAAGGCCTTCTTTGAACATGGAAAGATAGACTTATATAACATCTATGCTCCATTGTGTCATGACTCCTCTCTGAAAAATGGTTCAACTGGTTATGTAAGTAATGATTTTGACCCTTGTTCTGATTATTATGGTTCTGCCTATCTAAATAGACCAGAGGTTCAAAAGGCTCTTCATGCAAAACCTGCAAATTGGACCCATTGCAGGTAATATTCTATAATCACTTTTAAGAAATTCTAAAATAGTACTATTTTTTTGACACTTACATAAATTTATTTTTCTATGCTAATCAAGAATGTGGTATTTTTTATGACAATTCATAGTCGTCTTCTTACTGACTGGAAAGACAGTCCAATTACCATCTTACCAACTGTCAAGTATCTCATCGATAGCGGCATTAAATTATGGATATACAGGCAAGTTCACTTTCATTATAATATAAATTCTTAACACTGTTATCACAATTTAAATCTTCAAGAACAATATTATAATTATAACATGTAGACTATTGTGAATAATGACTAATGAGAGGTTCCAACTCCTAAATTTTGATGGTGCGTTCTCCTACAGTGGTGATACAGATGCAGTGGTTTCAGTTACATCTTCAAGATATTCAATCAACACCCTTAAGCTCCCTATAAACGCAGCTTGGCGTCCATGGTATTCTGGAAAAGAGGTACTGAAATTAATTTATTCGTAGTTTGATGGAGAAACTAAACTAATTGACATTCTATAATTTAAGAGACTGATTTGTTATTTTGTAAATTTGAGGGACAATTGACACTTACAATGTCGAGTACAAAAATTCTAATTCACTCCATATGTGGTTGATTTATTACCTTTTCTTGTATACCAAGTAATAACATTTTTGAAATGTGATCAAATTGTAATAATAATGACAGATTGGAGGATATGTGGTGGGATACAAAGGATTGACATTTGTTACAGTGAGAGGAGCAGGACATCTTGTTCCAAGCTGGCAACCAGAACGGGCTTTGACAATGATCTCATCATTCCTCTATGGAAGTTTGCCTCCTAGTTCACCATCAAATTAA
Protein sequence

>MS.gene25854.t1

MKKVSLYACLLLNLSLLVIFPYSKASQADKLNEFILSRKSQNPPKTLSWEVEDAVKTLSSSAADVAPQEGLMQADKIDTLPGQPYGVNFEQYSGYVTVDPEAGRELFYYFVESPYNPSTKPLVLWLNGGPGCSSLGYGAFQELGPFRINSDGKTLYRNPYSWNEVANILFLESPAGVGFSYSNTTSDYDKSGDKSTAKDAYVFLINWLERFPQYKTRDFYITGESYAGHYVPQLASTILHNNKLYNNTVINLKGISIGNAWIDDATSLKGFFDYLWTHALNSDQTHELIEKYCDFTTENVSAICINNVTLKAFFEHGKIDLYNIYAPLCHDSSLKNGSTGYVSNDFDPCSDYYGSAYLNRPEVQKALHAKPANWTHCSRLLTDWKDSPITILPTVKYLIDSGIKLWIYSGDTDAVVSVTSSRYSINTLKLPINAAWRPWYSGKEIGGYVVGYKGLTFVTVRGAGHLVPSWQPERALTMISSFLYGSLPPSSPSN