Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26151.t1 | XP_003616194.1 | 100 | 250 | 0 | 0 | 1 | 250 | 1 | 250 | 8.60E-144 | 520 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26151.t1 | Q8RWB1 | 81.3 | 252 | 43 | 3 | 2 | 252 | 3 | 251 | 7.2e-115 | 415.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26151.t1 | G7KFW5 | 100.0 | 250 | 0 | 0 | 1 | 250 | 1 | 250 | 6.2e-144 | 520.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049861 | MS.gene26151 | 0.807026 | 6.18E-50 | -1.69E-46 |
| MS.gene04991 | MS.gene26151 | -0.80875 | 2.66E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene26151 | 0.803003 | 4.29E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene26151 | 0.827534 | 1.48E-54 | -1.69E-46 |
| MS.gene052434 | MS.gene26151 | 0.840941 | 6.42E-58 | -1.69E-46 |
| MS.gene056752 | MS.gene26151 | 0.812171 | 4.85E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene26151 | 0.848947 | 4.43E-60 | -1.69E-46 |
| MS.gene057999 | MS.gene26151 | 0.813576 | 2.39E-51 | -1.69E-46 |
| MS.gene06010 | MS.gene26151 | 0.821817 | 3.30E-53 | -1.69E-46 |
| MS.gene06011 | MS.gene26151 | 0.810003 | 1.43E-50 | -1.69E-46 |
| MS.gene060259 | MS.gene26151 | 0.800251 | 1.57E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26151.t1 | MTR_5g077180 | 100.000 | 252 | 0 | 0 | 1 | 252 | 1 | 252 | 0.0 | 542 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26151.t1 | AT5G37370 | 79.537 | 259 | 49 | 3 | 2 | 259 | 3 | 258 | 4.31e-151 | 431 |
| MS.gene26151.t1 | AT5G37370 | 77.220 | 259 | 47 | 4 | 2 | 259 | 3 | 250 | 1.37e-143 | 412 |
| MS.gene26151.t1 | AT5G37370 | 77.670 | 206 | 42 | 3 | 55 | 259 | 1 | 203 | 1.53e-113 | 333 |
| MS.gene26151.t1 | AT5G37370 | 89.583 | 96 | 10 | 0 | 2 | 97 | 3 | 98 | 1.84e-60 | 190 |
| MS.gene26151.t1 | AT5G37370 | 89.583 | 96 | 10 | 0 | 2 | 97 | 3 | 98 | 1.84e-60 | 190 |
| MS.gene26151.t1 | AT5G37370 | 88.542 | 96 | 11 | 0 | 2 | 97 | 3 | 98 | 4.30e-59 | 186 |
| MS.gene26151.t1 | AT5G37370 | 88.542 | 96 | 11 | 0 | 2 | 97 | 3 | 98 | 4.30e-59 | 186 |
| MS.gene26151.t1 | AT5G37370 | 89.474 | 95 | 10 | 0 | 2 | 96 | 3 | 97 | 1.33e-58 | 184 |
| MS.gene26151.t1 | AT5G37370 | 89.474 | 95 | 10 | 0 | 2 | 96 | 3 | 97 | 1.33e-58 | 184 |
| MS.gene26151.t1 | AT5G37370 | 70.312 | 128 | 34 | 3 | 133 | 259 | 1 | 125 | 4.77e-54 | 179 |
| MS.gene26151.t1 | AT5G37370 | 70.312 | 128 | 34 | 3 | 133 | 259 | 1 | 125 | 4.77e-54 | 179 |
| MS.gene26151.t1 | AT5G37370 | 70.312 | 128 | 34 | 3 | 133 | 259 | 1 | 125 | 4.77e-54 | 179 |
Find 98 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGCCCTTTCAGTCTCTTT+TGG | 0.266936 | 5.4:+57265608 | MS.gene26151:CDS |
| TCACCACTATTATCCCTTCT+AGG | 0.271633 | 5.4:-57266244 | None:intergenic |
| TTAGACTTGGTGGCTCTTCT+TGG | 0.285751 | 5.4:+57266275 | MS.gene26151:CDS |
| AACCAGCTCCACAGAGTCTT+TGG | 0.285766 | 5.4:-57262859 | None:intergenic |
| GCTCTGACTGCGATGATTGC+TGG | 0.309396 | 5.4:-57265735 | None:intergenic |
| ACCTGACGCATTACAGGAAC+TGG | 0.327476 | 5.4:-57265476 | None:intergenic |
| GCCACAACCTGACGCATTAC+AGG | 0.330998 | 5.4:-57265482 | None:intergenic |
| GCGTCAGGTTGTGGCAAATC+TGG | 0.336533 | 5.4:+57265490 | MS.gene26151:CDS |
| GCTCTTATGTAAGGAGAATC+AGG | 0.377200 | 5.4:-57261423 | None:intergenic |
| CATGGCGTGCGGTGCCAACT+TGG | 0.388924 | 5.4:-57266096 | None:intergenic |
| TATATCCGTGATTTGCTCCT+TGG | 0.397027 | 5.4:+57263791 | MS.gene26151:CDS |
| ATATCTCATCAATAACTTCA+TGG | 0.399120 | 5.4:-57261269 | None:intergenic |
| CTGCTTGACAGTGAGCTTCA+TGG | 0.403160 | 5.4:-57261379 | None:intergenic |
| ATCATGGAGATACAGACTTC+TGG | 0.414622 | 5.4:+57261156 | None:intergenic |
| TCCTGTAATGCGTCAGGTTG+TGG | 0.416923 | 5.4:+57265481 | MS.gene26151:CDS |
| CCGTGATTTGCTCCTTGGAC+AGG | 0.429155 | 5.4:+57263796 | MS.gene26151:CDS |
| TTCTTCACCCATTCGTCGTA+CGG | 0.437084 | 5.4:+57265664 | MS.gene26151:CDS |
| CTCTGACTGCGATGATTGCT+GGG | 0.437763 | 5.4:-57265734 | None:intergenic |
| TTCTCTAGCAAGGACTCAAT+TGG | 0.441660 | 5.4:-57261183 | None:intergenic |
| ACCTCATCATCCTTTGCATA+TGG | 0.450018 | 5.4:-57262886 | None:intergenic |
| ACATGGAAAGAATTCCTAGA+AGG | 0.450119 | 5.4:+57266230 | MS.gene26151:CDS |
| ATCCAAAGACTCTGTGGAGC+TGG | 0.453230 | 5.4:+57262857 | MS.gene26151:CDS |
| CCCAGTTCCTGTAATGCGTC+AGG | 0.457842 | 5.4:+57265475 | MS.gene26151:CDS |
| TGCCAACTTGGCTCTGACTT+CGG | 0.478380 | 5.4:-57266084 | None:intergenic |
| AGAAGACAGAAAGCAGTTGA+AGG | 0.479991 | 5.4:-57261342 | None:intergenic |
| CGCCACATCGAGCCTCTACA+AGG | 0.483631 | 5.4:+57265639 | MS.gene26151:CDS |
| CTACATACCGCTCTTATGTA+AGG | 0.483855 | 5.4:-57261432 | None:intergenic |
| ACAGCATTGGCAATGGTAGT+AGG | 0.483968 | 5.4:+57266020 | MS.gene26151:CDS |
| CTTTCACAGAAGGTGGTCTA+CGG | 0.486987 | 5.4:-57265586 | None:intergenic |
| TCTAGGAATTCTTTCCATGT+TGG | 0.493850 | 5.4:-57266227 | None:intergenic |
| GGTGAAGAGGTTATTAGACT+TGG | 0.496955 | 5.4:+57266262 | MS.gene26151:CDS |
| GTCGTACCACAATGTGTAGT+AGG | 0.500096 | 5.4:-57265527 | None:intergenic |
| CATGTGGAACCATGGATGAC+GGG | 0.500480 | 5.4:+57261306 | MS.gene26151:CDS |
| CTCACTGTCAAGCAGATGCA+TGG | 0.508388 | 5.4:+57261387 | MS.gene26151:CDS |
| GCAGGTACCGTACGACGAAT+GGG | 0.512160 | 5.4:-57265671 | None:intergenic |
| AGCAGGTACCGTACGACGAA+TGG | 0.515431 | 5.4:-57265672 | None:intergenic |
| GAAGAGGTTATTAGACTTGG+TGG | 0.517064 | 5.4:+57266265 | MS.gene26151:CDS |
| ACGCAGAGAGAGAAAGTAGC+CGG | 0.520155 | 5.4:+57265987 | MS.gene26151:CDS |
| GTCATCCGTCCATTAGATCC+AGG | 0.523423 | 5.4:-57263755 | None:intergenic |
| CGCTCTAGTCCACAGAGAGA+TGG | 0.529001 | 5.4:+57266124 | MS.gene26151:CDS |
| CGACAGTTCCCCGTCATCCA+TGG | 0.535024 | 5.4:-57261315 | None:intergenic |
| AGCCGAAGTCAGAGCCAAGT+TGG | 0.536221 | 5.4:+57266082 | MS.gene26151:CDS |
| ACTACACATTGTGGTACGAC+AGG | 0.536972 | 5.4:+57265530 | MS.gene26151:CDS |
| TTTCACAGAAGGTGGTCTAC+GGG | 0.540490 | 5.4:-57265585 | None:intergenic |
| AGTGTCATCAGAACCGTGAC+GGG | 0.541768 | 5.4:-57265561 | None:intergenic |
| CGCAGAGAGAGAAAGTAGCC+GGG | 0.549213 | 5.4:+57265988 | MS.gene26151:CDS |
| AAGGGATAATAGTGGTGAAG+AGG | 0.549270 | 5.4:+57266249 | MS.gene26151:CDS |
| GTCGGAGTAGAAGTAGGAGC+AGG | 0.556706 | 5.4:+57266059 | MS.gene26151:CDS |
| CGGGATTATGACAACAGCAT+TGG | 0.559737 | 5.4:+57266007 | MS.gene26151:CDS |
| AGAATGCTTGTATCTGATGC+CGG | 0.561175 | 5.4:+57266317 | MS.gene26151:CDS |
| CAATGCTGTTGTCATAATCC+CGG | 0.569029 | 5.4:-57266006 | None:intergenic |
| CATGGAAAGAATTCCTAGAA+GGG | 0.569552 | 5.4:+57266231 | MS.gene26151:CDS |
| GCTGACCAAAAGAGACTGAA+AGG | 0.571040 | 5.4:-57265613 | None:intergenic |
| GTAGGAGTCGGAGTAGAAGT+AGG | 0.573084 | 5.4:+57266053 | MS.gene26151:CDS |
| GTAGTAGGCGCCATCGCAGT+AGG | 0.582103 | 5.4:+57266035 | MS.gene26151:CDS |
| GCAAAGGACTTTCTCTAGCA+AGG | 0.583455 | 5.4:-57261193 | None:intergenic |
| GTGGACTAGAGCGTGAGTCA+TGG | 0.585031 | 5.4:-57266114 | None:intergenic |
| TCGTACCACAATGTGTAGTA+GGG | 0.586324 | 5.4:-57265526 | None:intergenic |
| TTTCTCCTGGATCTAATGGA+CGG | 0.588245 | 5.4:+57263750 | MS.gene26151:CDS |
| ATTCCTAGAAGGGATAATAG+TGG | 0.588421 | 5.4:+57266241 | MS.gene26151:CDS |
| GAAAGGGCAGCTTTCACAGA+AGG | 0.590339 | 5.4:-57265596 | None:intergenic |
| GGCGCCATCGCAGTAGGAGT+CGG | 0.590973 | 5.4:+57266041 | MS.gene26151:CDS |
| TTCTTACTTCCATCTCTCTG+TGG | 0.595516 | 5.4:-57266133 | None:intergenic |
| CTACACATTGTGGTACGACA+GGG | 0.597615 | 5.4:+57265531 | MS.gene26151:CDS |
| TACTCCGACTCCTACTGCGA+TGG | 0.600763 | 5.4:-57266045 | None:intergenic |
| ATGACAGAATATTCATGCAA+AGG | 0.601691 | 5.4:-57261209 | None:intergenic |
| TGGAGATACAGACTTCTGGC+AGG | 0.602465 | 5.4:+57261160 | MS.gene26151:CDS |
| CTGACCAAAAGAGACTGAAA+GGG | 0.606327 | 5.4:-57265612 | None:intergenic |
| AAGCAAAGGAGATGCCAACA+TGG | 0.607508 | 5.4:+57266213 | MS.gene26151:CDS |
| TATGACAACAGCATTGGCAA+TGG | 0.607613 | 5.4:+57266013 | MS.gene26151:CDS |
| TCACTGTCAAGCAGATGCAT+GGG | 0.608557 | 5.4:+57261388 | MS.gene26151:CDS |
| CAGTGTCATCAGAACCGTGA+CGG | 0.611533 | 5.4:-57265562 | None:intergenic |
| AAAGATAACGAACCTGTCCA+AGG | 0.612596 | 5.4:-57263808 | None:intergenic |
| CTGGTTTGAGCCATATGCAA+AGG | 0.614974 | 5.4:+57262876 | MS.gene26151:CDS |
| ATACAATCAAGTTGATCATG+TGG | 0.617743 | 5.4:+57261290 | MS.gene26151:CDS |
| AATGGACGGATGACTACGAT+GGG | 0.618551 | 5.4:+57263764 | MS.gene26151:CDS |
| ACAGGGGAAACCACCCGTCA+CGG | 0.619623 | 5.4:+57265548 | MS.gene26151:CDS |
| AGGGCAGCTTTCACAGAAGG+TGG | 0.621262 | 5.4:-57265593 | None:intergenic |
| CCTGTCCAAGGAGCAAATCA+CGG | 0.626413 | 5.4:-57263796 | None:intergenic |
| GCCATATGCAAAGGATGATG+AGG | 0.627558 | 5.4:+57262885 | MS.gene26151:CDS |
| TCATGTGGAACCATGGATGA+CGG | 0.627735 | 5.4:+57261305 | MS.gene26151:CDS |
| TGGATGACGGGGAACTGTCG+TGG | 0.628076 | 5.4:+57261318 | MS.gene26151:CDS |
| CTCTTATGTAAGGAGAATCA+GGG | 0.628997 | 5.4:-57261422 | None:intergenic |
| GGGTGAAGAATCCCTTGTAG+AGG | 0.636783 | 5.4:-57265651 | None:intergenic |
| AAGTTGATCATGTGGAACCA+TGG | 0.636947 | 5.4:+57261298 | MS.gene26151:CDS |
| TAATGGACGGATGACTACGA+TGG | 0.637953 | 5.4:+57263763 | MS.gene26151:CDS |
| ATTACAGGAACTGGGATGCG+AGG | 0.640293 | 5.4:-57265467 | None:intergenic |
| CCTGACGCATTACAGGAACT+GGG | 0.642825 | 5.4:-57265475 | None:intergenic |
| ATGCTGATCCAAAGACTCTG+TGG | 0.643414 | 5.4:+57262851 | MS.gene26151:CDS |
| AAACTCCCTACTACACATTG+TGG | 0.647894 | 5.4:+57265521 | MS.gene26151:CDS |
| GTCATCAGAACCGTGACGGG+TGG | 0.647960 | 5.4:-57265558 | None:intergenic |
| ATGTGGAACCATGGATGACG+GGG | 0.653377 | 5.4:+57261307 | MS.gene26151:CDS |
| AGAGCGTGAGTCATGGCGTG+CGG | 0.670383 | 5.4:-57266107 | None:intergenic |
| TGATTCTCCTTACATAAGAG+CGG | 0.678807 | 5.4:+57261425 | MS.gene26151:CDS |
| TCCCTTGTAGAGGCTCGATG+TGG | 0.691841 | 5.4:-57265641 | None:intergenic |
| TACACATTGTGGTACGACAG+GGG | 0.693296 | 5.4:+57265532 | MS.gene26151:CDS |
| GGTGATTTAGGTGAAAGCAA+AGG | 0.714464 | 5.4:+57266199 | MS.gene26151:intron |
| GCCACATCGAGCCTCTACAA+GGG | 0.725791 | 5.4:+57265640 | MS.gene26151:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 57261159 | 57266343 | 57261159 | ID=MS.gene26151 |
| chr5.4 | mRNA | 57261159 | 57266343 | 57261159 | ID=MS.gene26151.t1;Parent=MS.gene26151 |
| chr5.4 | exon | 57261159 | 57261446 | 57261159 | ID=MS.gene26151.t1.exon1;Parent=MS.gene26151.t1 |
| chr5.4 | CDS | 57261159 | 57261446 | 57261159 | ID=cds.MS.gene26151.t1;Parent=MS.gene26151.t1 |
| chr5.4 | exon | 57262826 | 57262906 | 57262826 | ID=MS.gene26151.t1.exon2;Parent=MS.gene26151.t1 |
| chr5.4 | CDS | 57262826 | 57262906 | 57262826 | ID=cds.MS.gene26151.t1;Parent=MS.gene26151.t1 |
| chr5.4 | exon | 57263746 | 57263817 | 57263746 | ID=MS.gene26151.t1.exon3;Parent=MS.gene26151.t1 |
| chr5.4 | CDS | 57263746 | 57263817 | 57263746 | ID=cds.MS.gene26151.t1;Parent=MS.gene26151.t1 |
| chr5.4 | exon | 57265446 | 57265758 | 57265446 | ID=MS.gene26151.t1.exon4;Parent=MS.gene26151.t1 |
| chr5.4 | CDS | 57265446 | 57265758 | 57265446 | ID=cds.MS.gene26151.t1;Parent=MS.gene26151.t1 |
| chr5.4 | exon | 57265976 | 57266148 | 57265976 | ID=MS.gene26151.t1.exon5;Parent=MS.gene26151.t1 |
| chr5.4 | CDS | 57265976 | 57266148 | 57265976 | ID=cds.MS.gene26151.t1;Parent=MS.gene26151.t1 |
| chr5.4 | exon | 57266209 | 57266343 | 57266209 | ID=MS.gene26151.t1.exon6;Parent=MS.gene26151.t1 |
| chr5.4 | CDS | 57266209 | 57266343 | 57266209 | ID=cds.MS.gene26151.t1;Parent=MS.gene26151.t1 |
| Gene Sequence |
| Protein sequence |
