AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27083

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27083.t1 MTR_1g025420 96.330 327 6 2 1 325 1 323 0.0 636
MS.gene27083.t1 MTR_1g025420 98.031 254 1 1 1 254 1 250 0.0 503
MS.gene27083.t1 MTR_3g114030 80.591 237 46 0 21 257 1 237 1.64e-143 404
MS.gene27083.t1 MTR_8g037890 59.797 296 104 7 5 298 6 288 2.14e-119 347
MS.gene27083.t1 MTR_5g022310 68.852 244 72 3 15 255 14 256 4.74e-118 342
MS.gene27083.t1 MTR_5g022310 69.008 242 71 3 15 253 14 254 2.03e-117 339
MS.gene27083.t1 MTR_8g075550 70.464 237 63 3 20 251 21 255 2.23e-116 338
MS.gene27083.t1 MTR_4g073730 52.648 321 138 5 6 324 3 311 6.26e-114 333
MS.gene27083.t1 MTR_8g056820 63.602 261 88 5 1 260 1 255 7.30e-113 328
MS.gene27083.t1 MTR_4g073730 57.447 282 109 4 6 285 3 275 1.05e-111 326
MS.gene27083.t1 MTR_4g073720 64.876 242 74 5 8 249 9 239 5.28e-104 308
MS.gene27083.t1 MTR_5g022350 51.420 317 139 7 3 318 9 311 9.11e-100 296
MS.gene27083.t1 MTR_8g075510 61.044 249 87 5 3 251 7 245 5.40e-99 292
MS.gene27083.t1 MTR_8g075510 61.134 247 86 5 3 249 7 243 8.48e-99 294
MS.gene27083.t1 MTR_7g102380 55.230 239 104 3 11 249 5 240 3.51e-94 280
MS.gene27083.t1 MTR_1g062660 56.175 251 102 5 1 251 1 243 7.46e-87 261
MS.gene27083.t1 MTR_8g036215 54.508 244 101 6 6 249 4 237 1.96e-86 259
MS.gene27083.t1 MTR_1g062590 52.263 243 113 3 7 249 2 241 2.03e-86 260
MS.gene27083.t1 MTR_2g069660 53.333 240 105 4 13 249 7 242 3.46e-86 259
MS.gene27083.t1 MTR_1g062350 56.017 241 97 4 11 251 12 243 1.47e-85 258
MS.gene27083.t1 MTR_2g068655 52.610 249 113 4 3 249 6 251 1.61e-85 260
MS.gene27083.t1 MTR_1g062370 55.602 241 98 4 11 251 12 243 1.79e-85 258
MS.gene27083.t1 MTR_2g067980 53.876 258 108 7 11 263 7 258 3.30e-85 277
MS.gene27083.t1 MTR_2g068030 54.978 231 98 5 21 249 17 243 1.12e-84 256
MS.gene27083.t1 MTR_1g062630 54.357 241 101 4 11 251 12 243 2.72e-84 255
MS.gene27083.t1 MTR_1g062340 53.846 247 107 4 5 251 4 243 2.97e-84 254
MS.gene27083.t1 MTR_6g079580 50.833 240 113 4 10 249 5 239 4.95e-80 243
MS.gene27083.t1 MTR_1g021945 57.522 226 88 6 26 249 25 244 1.25e-79 243
MS.gene27083.t1 MTR_8g107140 52.917 240 108 4 10 249 10 244 1.30e-79 243
MS.gene27083.t1 MTR_3g111620 52.632 247 108 8 11 255 10 249 2.41e-74 229
MS.gene27083.t1 MTR_3g068015 48.387 248 117 8 8 249 5 247 4.55e-70 218
MS.gene27083.t1 MTR_1g062690 50.207 241 87 5 11 251 12 219 1.02e-69 216
MS.gene27083.t1 MTR_5g059200 48.810 252 115 9 1 250 1 240 4.54e-69 217
MS.gene27083.t1 MTR_6g009480 43.548 248 132 8 5 249 9 251 6.81e-64 203
MS.gene27083.t1 MTR_7g062610 46.531 245 125 6 9 252 5 244 7.33e-64 202
MS.gene27083.t1 MTR_4g063630 44.444 225 117 7 29 249 31 251 7.94e-61 195
MS.gene27083.t1 MTR_5g023850 54.237 177 76 3 14 187 13 187 4.50e-60 192
MS.gene27083.t1 MTR_2g067990 47.186 231 88 9 22 249 22 221 1.90e-55 194
MS.gene27083.t1 MTR_5g010640 40.964 249 118 8 8 249 9 235 7.27e-50 166
MS.gene27083.t1 MTR_2g067970 46.701 197 94 8 69 261 2 191 7.48e-50 178
MS.gene27083.t1 MTR_5g022350 56.329 158 60 5 2 158 73 222 2.96e-48 161
MS.gene27083.t1 MTR_5g010635 40.000 225 111 7 26 249 23 224 2.23e-46 157
MS.gene27083.t1 MTR_8g096900 39.918 243 118 9 10 249 30 247 5.85e-46 156
MS.gene27083.t1 MTR_7g076360 38.318 214 67 4 26 239 7 155 2.10e-39 137
MS.gene27083.t1 MTR_8g089020 52.672 131 56 3 111 241 32 156 4.00e-38 133
MS.gene27083.t1 MTR_2g063150 31.276 243 151 6 10 243 9 244 4.25e-37 134
MS.gene27083.t1 MTR_1g062390 32.000 250 149 7 7 251 6 239 3.96e-32 120
MS.gene27083.t1 MTR_3g081550 57.447 94 40 0 112 205 16 109 1.69e-30 112
MS.gene27083.t1 MTR_8g096910 43.478 138 66 6 8 142 1 129 4.51e-26 102
MS.gene27083.t1 MTR_8g096920 40.769 130 67 4 17 145 9 129 2.41e-21 89.7
MS.gene27083.t1 MTR_1g062640 44.792 96 51 2 73 168 53 146 5.76e-18 80.1
MS.gene27083.t1 MTR_3g114040 56.944 72 27 2 255 325 33 101 8.45e-16 72.4
MS.gene27083.t1 MTR_5g068670 43.750 80 42 2 65 144 13 89 3.84e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27083.t1 AT1G75800 63.609 327 95 4 13 321 9 329 1.75e-147 418
MS.gene27083.t1 AT1G20030 66.557 305 96 3 23 325 16 316 5.98e-144 409
MS.gene27083.t1 AT1G20030 66.337 303 96 3 25 325 1 299 1.25e-142 405
MS.gene27083.t1 AT4G36010 63.736 273 91 4 23 289 19 289 9.06e-118 342
MS.gene27083.t1 AT4G36010 63.736 273 91 4 23 289 19 289 9.06e-118 342
MS.gene27083.t1 AT4G38670 61.446 249 95 1 10 257 6 254 2.24e-110 322
MS.gene27083.t1 AT4G38670 61.446 249 95 1 10 257 6 254 2.24e-110 322
MS.gene27083.t1 AT4G38670 62.605 238 88 1 21 257 1 238 4.66e-108 316
MS.gene27083.t1 AT2G17860 62.857 245 86 4 13 253 9 252 5.17e-107 313
MS.gene27083.t1 AT4G38660 62.109 256 88 5 17 272 20 266 4.64e-102 303
MS.gene27083.t1 AT4G38660 62.302 252 86 5 21 272 2 244 5.63e-101 300
MS.gene27083.t1 AT4G24180 56.631 279 109 4 3 275 9 281 6.41e-99 294
MS.gene27083.t1 AT4G24180 59.608 255 91 4 3 251 9 257 9.22e-98 290
MS.gene27083.t1 AT4G24180 57.196 271 104 4 11 275 12 276 9.43e-98 291
MS.gene27083.t1 AT5G38280 54.373 263 113 4 13 269 11 272 3.39e-97 301
MS.gene27083.t1 AT5G38280 54.373 263 113 4 13 269 11 272 5.12e-97 301
MS.gene27083.t1 AT4G24180 60.324 247 86 4 11 251 12 252 1.94e-96 286
MS.gene27083.t1 AT4G24180 59.307 231 83 3 10 234 16 241 8.21e-89 269
MS.gene27083.t1 AT4G24180 58.696 230 84 3 11 234 12 236 7.98e-88 266
MS.gene27083.t1 AT1G19320 57.831 249 89 9 8 250 9 247 3.10e-87 262
MS.gene27083.t1 AT1G75030 58.442 231 88 7 26 255 23 246 5.24e-84 254
MS.gene27083.t1 AT1G75050 56.962 237 97 5 13 249 11 242 6.51e-84 254
MS.gene27083.t1 AT1G75040 56.148 244 99 6 6 249 3 238 9.11e-84 253
MS.gene27083.t1 AT1G18250 54.464 224 97 4 26 249 20 238 6.87e-80 243
MS.gene27083.t1 AT5G24620 51.639 244 111 5 11 249 9 250 7.64e-80 249
MS.gene27083.t1 AT1G18250 54.464 224 97 4 26 249 21 239 8.09e-80 243
MS.gene27083.t1 AT1G73620 55.357 224 95 4 26 249 41 259 1.10e-79 243
MS.gene27083.t1 AT5G24620 48.669 263 117 6 3 249 45 305 1.11e-79 250
MS.gene27083.t1 AT5G24620 48.669 263 117 6 3 249 66 326 1.30e-79 251
MS.gene27083.t1 AT1G77700 51.304 230 92 9 26 251 60 273 3.03e-70 221
MS.gene27083.t1 AT1G77700 51.327 226 98 8 26 251 60 273 1.14e-69 219
MS.gene27083.t1 AT1G77700 51.327 226 98 8 26 251 88 301 1.35e-69 221
MS.gene27083.t1 AT2G28790 47.107 242 120 7 12 249 36 273 3.65e-67 212
MS.gene27083.t1 AT2G28790 47.107 242 120 7 12 249 11 248 4.62e-67 211
MS.gene27083.t1 AT1G70250 47.083 240 114 6 25 258 143 375 2.68e-65 220
MS.gene27083.t1 AT4G36000 62.644 174 53 5 103 274 1 164 9.17e-65 203
MS.gene27083.t1 AT4G36000 62.644 174 53 5 103 274 1 164 9.17e-65 203
MS.gene27083.t1 AT4G18253 43.443 244 128 4 13 255 9 243 1.32e-64 204
MS.gene27083.t1 AT5G40020 46.256 227 117 5 26 251 27 249 3.14e-63 201
MS.gene27083.t1 AT4G18250 44.030 268 139 6 13 277 42 301 3.37e-62 209
MS.gene27083.t1 AT5G02140 44.177 249 133 6 14 260 7 251 2.08e-61 197
MS.gene27083.t1 AT5G02140 45.798 238 123 6 14 249 7 240 1.13e-60 194
MS.gene27083.t1 AT4G11650 39.506 243 122 8 8 249 4 222 2.26e-49 165
MS.gene27083.t1 AT5G24620 56.667 120 47 3 134 249 15 133 1.42e-37 136
MS.gene27083.t1 AT2G24810 62.195 82 26 3 170 250 116 193 8.61e-27 104

Find 95 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCATGGGGCGGCCGTTTC+TGG -0.083846 1.1:-541276 MS.gene27083:CDS
GCACTTCATCTATCAATTTC+AGG 0.196637 1.1:-541571 MS.gene27083:intron
GCCAGAGCATTCAACCTTTC+CGG 0.211647 1.1:+541189 None:intergenic
CTCATGGGGCGGCCGTTTCT+GGG 0.253028 1.1:-541275 MS.gene27083:CDS
CCAGGGATTCTTTCAAATGC+TGG 0.316773 1.1:-541367 MS.gene27083:CDS
AACAATTGAGAATAAGTAGA+AGG 0.345262 1.1:+540860 None:intergenic
CAGGGATTCTTTCAAATGCT+GGG 0.347178 1.1:-541366 MS.gene27083:CDS
CAGTGGTGGTTTCGCCGGTT+TGG 0.373152 1.1:+541311 None:intergenic
TATTGCTGCAGCGGCGCGTA+TGG 0.373787 1.1:-540902 MS.gene27083:CDS
TTCGATGTTAGTCTTGTTGA+CGG 0.393312 1.1:-541100 MS.gene27083:CDS
GGAAAATTCTCATGTGTAAC+CGG 0.404051 1.1:-541223 MS.gene27083:CDS
TGCACGCAAAAGTTTGTGAT+TGG 0.405228 1.1:+539738 None:intergenic
GTCGCCCCTCAGGGCGGCTC+TGG 0.409338 1.1:-541058 MS.gene27083:CDS
TCTCAACGGCGCGTGTCCTT+CGG 0.414761 1.1:-541002 MS.gene27083:CDS
TTAGCATCACTATGGTCCTT+TGG 0.416286 1.1:-539712 MS.gene27083:CDS
GCCTATGCTAGTCGCCCCTC+AGG 0.418663 1.1:-541068 MS.gene27083:CDS
TCCGGAAAGGTTGAATGCTC+TGG 0.437194 1.1:-541190 MS.gene27083:CDS
GTGAATTCGGCTAAAGTTGC+CGG 0.439691 1.1:+541151 None:intergenic
AGAGCCGTCTAATGTGAATT+CGG 0.464663 1.1:+541138 None:intergenic
TGTCACCGCACCAGCCTCAT+GGG 0.466682 1.1:-541290 MS.gene27083:CDS
GTGCATTATTAGCATCACTA+TGG 0.467599 1.1:-539720 MS.gene27083:CDS
ACAGGCTTTGTTCTCCAAAC+CGG 0.470298 1.1:-541325 MS.gene27083:CDS
GTAACCGGCGATTGTGGCTC+CGG 0.470556 1.1:-541208 MS.gene27083:CDS
GTAGAAGGTTTGCAAGTGTC+TGG 0.470850 1.1:+540875 None:intergenic
AAACGGCCGCCCCATGAGGC+TGG 0.484136 1.1:+541280 None:intergenic
CTGTCACCGCACCAGCCTCA+TGG 0.485359 1.1:-541291 MS.gene27083:CDS
CCAGCATTTGAAAGAATCCC+TGG 0.493036 1.1:+541367 None:intergenic
TGTGACTACACAGTGTGGCC+AGG 0.496467 1.1:-541385 MS.gene27083:CDS
GGTGACAGTGGTGGTTTCGC+CGG 0.497671 1.1:+541306 None:intergenic
AAAGAGTTCTGCCCCAGAAA+CGG 0.509995 1.1:+541263 None:intergenic
TCATGGGGCGGCCGTTTCTG+GGG 0.511165 1.1:-541274 MS.gene27083:CDS
GAAGATCTCATTAGAAGAGT+TGG 0.513485 1.1:+539681 None:intergenic
GCATCACTATGGTCCTTTGG+CGG 0.514552 1.1:-539709 MS.gene27083:CDS
AAAGTAACTAACCTTGTGGT+TGG 0.518082 1.1:+540734 None:intergenic
AAGCCTGTGGTTGGAAGTGT+TGG 0.519230 1.1:+541340 None:intergenic
TATCGCCAGAGCCGCCCTGA+GGG 0.521997 1.1:+541053 None:intergenic
CCCTGAGGGGCGACTAGCAT+AGG 0.525025 1.1:+541067 None:intergenic
TCATGTGTAACCGGCGATTG+TGG 0.527231 1.1:-541214 MS.gene27083:CDS
GAATTCACATTAGACGGCTC+TGG 0.528881 1.1:-541136 MS.gene27083:CDS
CTTTGCTCCCAAGACACTGA+TGG 0.532188 1.1:-541244 MS.gene27083:CDS
TTCACATTAGACGGCTCTGG+TGG 0.532251 1.1:-541133 MS.gene27083:CDS
ATTGCTGCAGCGGCGCGTAT+GGG 0.534091 1.1:-540901 MS.gene27083:CDS
GGCGATAATTGCACTACCAC+GGG 0.537099 1.1:-541037 MS.gene27083:CDS
GGCTAAAGTTGCCGGCGGTG+TGG 0.544415 1.1:+541159 None:intergenic
TTCAGTCAAAAGGCAACACA+AGG 0.545590 1.1:-539887 MS.gene27083:intron
ATAGCTATATGCACGTGGGC+AGG 0.548259 1.1:+540829 None:intergenic
CTTAGAGTGATGAGTGTGGA+TGG 0.554333 1.1:-540977 MS.gene27083:CDS
TGGAGAACAAAGCCTGTGGT+TGG 0.555388 1.1:+541331 None:intergenic
CCTATGCTAGTCGCCCCTCA+GGG 0.555587 1.1:-541067 MS.gene27083:CDS
AGGGATTCTTTCAAATGCTG+GGG 0.560190 1.1:-541365 MS.gene27083:CDS
GCCGCCCCATGAGGCTGGTG+CGG 0.562230 1.1:+541285 None:intergenic
GCGCCGCTGCAGCAATACTC+CGG 0.562265 1.1:+540908 None:intergenic
CTTTCCGGAGCCACAATCGC+CGG 0.563498 1.1:+541204 None:intergenic
CGGCGATTGTGGCTCCGGAA+AGG 0.566510 1.1:-541203 MS.gene27083:CDS
AAGTTTGTGATTGGAGCACA+TGG 0.568630 1.1:+539747 None:intergenic
GTGACTACACAGTGTGGCCA+GGG 0.569936 1.1:-541384 MS.gene27083:CDS
GTCCCAACACTTCCAACCAC+AGG 0.572652 1.1:-541343 MS.gene27083:CDS
AAGGTTGAATGCTCTGGCAA+CGG 0.576244 1.1:-541184 MS.gene27083:CDS
TTAGCCGAATTCACATTAGA+CGG 0.576825 1.1:-541142 MS.gene27083:CDS
TGGCAACGGAGCCACACCGC+CGG 0.578628 1.1:-541170 MS.gene27083:CDS
ACCGCACCAGCCTCATGGGG+CGG 0.586044 1.1:-541286 MS.gene27083:CDS
ATGAGTGTGGATGGGAAAGA+CGG 0.592613 1.1:-540968 MS.gene27083:CDS
TGGCGATAATTGCACTACCA+CGG 0.593427 1.1:-541038 MS.gene27083:CDS
GACAATACAATGGTGTATGA+AGG 0.596541 1.1:-539809 MS.gene27083:CDS
CCAGAAACGGCCGCCCCATG+AGG 0.596649 1.1:+541276 None:intergenic
TCACCGGAGTATTGCTGCAG+CGG 0.602026 1.1:-540911 MS.gene27083:CDS
AGAGTTGGCACAACCGCCAA+AGG 0.605702 1.1:+539696 None:intergenic
ATTTGCACAAGTGAAAGTGC+TGG 0.609107 1.1:+540790 None:intergenic
TTGGAGCACATGGGTGCACA+TGG 0.612628 1.1:+539757 None:intergenic
TTATCGCCAGAGCCGCCCTG+AGG 0.617214 1.1:+541052 None:intergenic
AGCCTGTGGTTGGAAGTGTT+GGG 0.621679 1.1:+541341 None:intergenic
TCACATTAGACGGCTCTGGT+GGG 0.623574 1.1:-541132 MS.gene27083:CDS
TGAGGCTGGTGCGGTGACAG+TGG 0.623668 1.1:+541294 None:intergenic
ATCGCCAGAGCCGCCCTGAG+GGG 0.629555 1.1:+541054 None:intergenic
GTCATTGCTAAGCTGAGGTG+AGG 0.630131 1.1:+539829 None:intergenic
TTAGAGTGATGAGTGTGGAT+GGG 0.630518 1.1:-540976 MS.gene27083:CDS
GTCACCGCACCAGCCTCATG+GGG 0.632870 1.1:-541289 MS.gene27083:CDS
GGCTGGTGCGGTGACAGTGG+TGG 0.635446 1.1:+541297 None:intergenic
TTTGTTCTTCTCCAACCACA+AGG 0.638050 1.1:-540745 MS.gene27083:intron
CTCATCACTCTAAGCTCCGA+AGG 0.638536 1.1:+540986 None:intergenic
AAATTGATAGTACTTGGTGG+TGG 0.656416 1.1:+541608 None:intergenic
TAAGCATAGCTATATGCACG+TGG 0.665332 1.1:+540824 None:intergenic
GGATGTATCAGTGATCTCAA+CGG 0.673930 1.1:-541016 MS.gene27083:CDS
GCTTAGCAATGACAATACAA+TGG 0.678768 1.1:-539819 MS.gene27083:CDS
GAGATCACTGATACATCCCG+TGG 0.679790 1.1:+541021 None:intergenic
ATGCTAGTCGCCCCTCAGGG+CGG 0.689357 1.1:-541064 MS.gene27083:CDS
GTATTGTCATTGCTAAGCTG+AGG 0.695826 1.1:+539824 None:intergenic
GGTTTGGAGAACAAAGCCTG+TGG 0.698538 1.1:+541327 None:intergenic
AATTCGGCTAAAGTTGCCGG+CGG 0.706419 1.1:+541154 None:intergenic
AATACAATGGTGTATGAAGG+TGG 0.707551 1.1:-539806 MS.gene27083:CDS
TCAGTCAAAAGGCAACACAA+GGG 0.710852 1.1:-539886 MS.gene27083:intron
AAGCATAGCTATATGCACGT+GGG 0.721806 1.1:+540825 None:intergenic
GGAGCTTAGAGTGATGAGTG+TGG 0.722376 1.1:-540981 MS.gene27083:CDS
ACAAATGTGACTACACAGTG+TGG 0.729139 1.1:-541390 MS.gene27083:CDS
AGTTTGTGATTGGAGCACAT+GGG 0.786907 1.1:+539748 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTATTAAATGTTTGTTTT+TGG + chr1.1:539791-539810 None:intergenic 15.0%
!! AATAAATTGTTCAATGAACT+CGG - chr1.1:540813-540832 MS.gene27083:CDS 20.0%
!! GACAAAATTCATTAATCATT+TGG + chr1.1:540789-540808 None:intergenic 20.0%
!! TAAGATTGATTATATTCAGT+AGG + chr1.1:540649-540668 None:intergenic 20.0%
!! TCATATTAGTTAAGATTTAC+CGG + chr1.1:540672-540691 None:intergenic 20.0%
!! TGTACATCAATCAATATAAT+TGG + chr1.1:541177-541196 None:intergenic 20.0%
!!! AATATAATTGGTTCTAGATA+TGG + chr1.1:541165-541184 None:intergenic 20.0%
!!! AGAACAATTTTTCTATGAAT+CGG - chr1.1:540911-540930 MS.gene27083:CDS 20.0%
!!! ATGAAAAAATTGATAGTACT+TGG + chr1.1:539708-539727 None:intergenic 20.0%
!!! TTATATTGATTGATGTACAT+TGG - chr1.1:541178-541197 MS.gene27083:CDS 20.0%
!!! TTTTTACGGTTATTATTTTG+AGG + chr1.1:540611-540630 None:intergenic 20.0%
! AAATAAAGTAACTAACCTTG+TGG + chr1.1:540580-540599 None:intergenic 25.0%
! AACAATTGAGAATAAGTAGA+AGG + chr1.1:540450-540469 None:intergenic 25.0%
! ATAAATTGTTCAATGAACTC+GGG - chr1.1:540814-540833 MS.gene27083:CDS 25.0%
! CTGAATATAATCAATCTTAC+CGG - chr1.1:540650-540669 MS.gene27083:intron 25.0%
! TCAATAGAAATTGATGCAAA+GGG - chr1.1:541013-541032 MS.gene27083:CDS 25.0%
! TTCAATAGAAATTGATGCAA+AGG - chr1.1:541012-541031 MS.gene27083:CDS 25.0%
!! AAAAAATTGATAGTACTTGG+TGG + chr1.1:539705-539724 None:intergenic 25.0%
!! ATAAAGTTCATCACACTTTT+TGG + chr1.1:541293-541312 None:intergenic 25.0%
!! CTATTGTATTTTCAGTCAAA+AGG - chr1.1:541410-541429 MS.gene27083:CDS 25.0%
!! TCACAATGTAATTTTCCTAA+CGG + chr1.1:541318-541337 None:intergenic 25.0%
!!! GCACAATGGTAAATTTTTTA+CGG + chr1.1:540625-540644 None:intergenic 25.0%
ACATTGGATTCGCAAAATAT+GGG - chr1.1:541194-541213 MS.gene27083:CDS 30.0%
AGAAAAATTGTTCTGCTGTA+AGG + chr1.1:540905-540924 None:intergenic 30.0%
GGTTGCAAGGTAAATTATAA+CGG + chr1.1:540869-540888 None:intergenic 30.0%
TACATTGGATTCGCAAAATA+TGG - chr1.1:541193-541212 MS.gene27083:CDS 30.0%
TCCAAAATATCCAATGTGAT+AGG - chr1.1:541116-541135 MS.gene27083:CDS 30.0%
TTATCGTTACACAACACTAT+TGG - chr1.1:541241-541260 MS.gene27083:CDS 30.0%
TTTGTGAATATGTCATATGC+AGG - chr1.1:539865-539884 MS.gene27083:CDS 30.0%
! ACAATTTTTCTATGAATCGG+AGG - chr1.1:540914-540933 MS.gene27083:CDS 30.0%
! ACCTATCACATTGGATATTT+TGG + chr1.1:541120-541139 None:intergenic 30.0%
! CAATTTTTCTATGAATCGGA+GGG - chr1.1:540915-540934 MS.gene27083:CDS 30.0%
! CTGTAAGGTAATTTAGAGTT+CGG + chr1.1:540890-540909 None:intergenic 30.0%
! GAGTTTGATTCGATTAGATT+GGG - chr1.1:540722-540741 MS.gene27083:intron 30.0%
!! ACATTCATAACTATTTCTGC+CGG + chr1.1:541089-541108 None:intergenic 30.0%
!! CACATTGGATATTTTGGATT+TGG + chr1.1:541114-541133 None:intergenic 30.0%
AAAGTAACTAACCTTGTGGT+TGG + chr1.1:540576-540595 None:intergenic 35.0%
AATACAATGGTGTATGAAGG+TGG - chr1.1:541501-541520 MS.gene27083:intron 35.0%
AATTGATTACCCACCAATCA+TGG + chr1.1:540993-541012 None:intergenic 35.0%
ATTGCTTGCAATTGCAAACA+TGG + chr1.1:541042-541061 None:intergenic 35.0%
GACAATACAATGGTGTATGA+AGG - chr1.1:541498-541517 MS.gene27083:intron 35.0%
GCACTTCATCTATCAATTTC+AGG - chr1.1:539736-539755 MS.gene27083:CDS 35.0%
GCTTAGCAATGACAATACAA+TGG - chr1.1:541488-541507 MS.gene27083:intron 35.0%
GGAAAATTCTCATGTGTAAC+CGG - chr1.1:540084-540103 MS.gene27083:intron 35.0%
GTGCATTATTAGCATCACTA+TGG - chr1.1:541587-541606 MS.gene27083:CDS 35.0%
TAGTAGGTGAAGCAACATAA+TGG - chr1.1:541063-541082 MS.gene27083:CDS 35.0%
TATATTCAGTAGGTGCACAA+TGG + chr1.1:540639-540658 None:intergenic 35.0%
TGATGAACTTTATGACCGTT+AGG - chr1.1:541300-541319 MS.gene27083:CDS 35.0%
TTAGCCGAATTCACATTAGA+CGG - chr1.1:540165-540184 MS.gene27083:intron 35.0%
! AAATTGATAGTACTTGGTGG+TGG + chr1.1:539702-539721 None:intergenic 35.0%
! AGAATTTTCCATCAGTGTCT+TGG + chr1.1:540074-540093 None:intergenic 35.0%
! GAATTTTCCATCAGTGTCTT+GGG + chr1.1:540073-540092 None:intergenic 35.0%
! GGAGTTTGATTCGATTAGAT+TGG - chr1.1:540721-540740 MS.gene27083:intron 35.0%
! TAATTTAGAGTTCGGTTGCA+AGG + chr1.1:540882-540901 None:intergenic 35.0%
!! ATGCGAAGCTTTTAATTCAC+CGG - chr1.1:540380-540399 MS.gene27083:intron 35.0%
!! TCCGAACTATTGGTTCATAA+GGG + chr1.1:540704-540723 None:intergenic 35.0%
!! TTCGATGTTAGTCTTGTTGA+CGG - chr1.1:540207-540226 MS.gene27083:intron 35.0%
AAGCATAGCTATATGCACGT+GGG + chr1.1:540485-540504 None:intergenic 40.0%
AAGTTTGTGATTGGAGCACA+TGG + chr1.1:541563-541582 None:intergenic 40.0%
ACAAATGTGACTACACAGTG+TGG - chr1.1:539917-539936 MS.gene27083:intron 40.0%
AGAGCCGTCTAATGTGAATT+CGG + chr1.1:540172-540191 None:intergenic 40.0%
AGGGATTCTTTCAAATGCTG+GGG - chr1.1:539942-539961 MS.gene27083:intron 40.0%
AGTTTGTGATTGGAGCACAT+GGG + chr1.1:541562-541581 None:intergenic 40.0%
ATGAACTCGGGTTCGATTAA+TGG - chr1.1:540826-540845 MS.gene27083:CDS 40.0%
CAGGGATTCTTTCAAATGCT+GGG - chr1.1:539941-539960 MS.gene27083:intron 40.0%
CCCCTTATGAACCAATAGTT+CGG - chr1.1:540700-540719 MS.gene27083:intron 40.0%
CGAATCAAACTCCGAACTAT+TGG + chr1.1:540714-540733 None:intergenic 40.0%
GCAATTGCAAGCAATCTAGT+AGG - chr1.1:541047-541066 MS.gene27083:CDS 40.0%
GGATGTATCAGTGATCTCAA+CGG - chr1.1:540291-540310 MS.gene27083:intron 40.0%
GTATTGTCATTGCTAAGCTG+AGG + chr1.1:541486-541505 None:intergenic 40.0%
TAAGCATAGCTATATGCACG+TGG + chr1.1:540486-540505 None:intergenic 40.0%
TCAGTCAAAAGGCAACACAA+GGG - chr1.1:541421-541440 MS.gene27083:CDS 40.0%
TGAACTCGGGTTCGATTAAT+GGG - chr1.1:540827-540846 MS.gene27083:CDS 40.0%
TGCACGCAAAAGTTTGTGAT+TGG + chr1.1:541572-541591 None:intergenic 40.0%
TTAGAGTGATGAGTGTGGAT+GGG - chr1.1:540331-540350 MS.gene27083:intron 40.0%
TTAGCATCACTATGGTCCTT+TGG - chr1.1:541595-541614 MS.gene27083:CDS 40.0%
TTCAGTCAAAAGGCAACACA+AGG - chr1.1:541420-541439 MS.gene27083:CDS 40.0%
TTGGATTCGCAAAATATGGG+TGG - chr1.1:541197-541216 MS.gene27083:CDS 40.0%
TTTGTTCTTCTCCAACCACA+AGG - chr1.1:540562-540581 MS.gene27083:intron 40.0%
! ATTTGCACAAGTGAAAGTGC+TGG + chr1.1:540520-540539 None:intergenic 40.0%
! TTTTAGCCACCATGATTGGT+GGG - chr1.1:540981-541000 MS.gene27083:CDS 40.0%
!! CCGAACTATTGGTTCATAAG+GGG + chr1.1:540703-540722 None:intergenic 40.0%
!! CTCCGAACTATTGGTTCATA+AGG + chr1.1:540705-540724 None:intergenic 40.0%
!! TGCTTTTTAGCCACCATGAT+TGG - chr1.1:540977-540996 MS.gene27083:CDS 40.0%
!! TTTTTAGCCACCATGATTGG+TGG - chr1.1:540980-540999 MS.gene27083:CDS 40.0%
AAAGAGTTCTGCCCCAGAAA+CGG + chr1.1:540047-540066 None:intergenic 45.0%
AAGGTTGAATGCTCTGGCAA+CGG - chr1.1:540123-540142 MS.gene27083:intron 45.0%
ACCAAGGAGACCTATCACAT+TGG + chr1.1:541129-541148 None:intergenic 45.0%
AGGTGAAGCAACATAATGGC+CGG - chr1.1:541067-541086 MS.gene27083:CDS 45.0%
ATGAGTGTGGATGGGAAAGA+CGG - chr1.1:540339-540358 MS.gene27083:intron 45.0%
CCAGCATTTGAAAGAATCCC+TGG + chr1.1:539943-539962 None:intergenic 45.0%
CCAGGGATTCTTTCAAATGC+TGG - chr1.1:539940-539959 MS.gene27083:intron 45.0%
CTTAGAGTGATGAGTGTGGA+TGG - chr1.1:540330-540349 MS.gene27083:intron 45.0%
GAATTCACATTAGACGGCTC+TGG - chr1.1:540171-540190 MS.gene27083:intron 45.0%
GTAGAAGGTTTGCAAGTGTC+TGG + chr1.1:540435-540454 None:intergenic 45.0%
GTGAATTCGGCTAAAGTTGC+CGG + chr1.1:540159-540178 None:intergenic 45.0%
TCCAATGTGATAGGTCTCCT+TGG - chr1.1:541125-541144 MS.gene27083:CDS 45.0%
TGATTACCCACCAATCATGG+TGG + chr1.1:540990-541009 None:intergenic 45.0%
TGGCGATAATTGCACTACCA+CGG - chr1.1:540269-540288 MS.gene27083:intron 45.0%
!! ACAGGCTTTGTTCTCCAAAC+CGG - chr1.1:539982-540001 MS.gene27083:intron 45.0%
AATTCGGCTAAAGTTGCCGG+CGG + chr1.1:540156-540175 None:intergenic 50.0%
ATAGCTATATGCACGTGGGC+AGG + chr1.1:540481-540500 None:intergenic 50.0%
CTCATCACTCTAAGCTCCGA+AGG + chr1.1:540324-540343 None:intergenic 50.0%
CTTTGCTCCCAAGACACTGA+TGG - chr1.1:540063-540082 MS.gene27083:intron 50.0%
GAGATCACTGATACATCCCG+TGG + chr1.1:540289-540308 None:intergenic 50.0%
GCATCACTATGGTCCTTTGG+CGG - chr1.1:541598-541617 MS.gene27083:CDS 50.0%
GGAGCTTAGAGTGATGAGTG+TGG - chr1.1:540326-540345 MS.gene27083:intron 50.0%
GGCGATAATTGCACTACCAC+GGG - chr1.1:540270-540289 MS.gene27083:intron 50.0%
GGTTTGGAGAACAAAGCCTG+TGG + chr1.1:539983-540002 None:intergenic 50.0%
GTAACGATAACCCTTGCCCT+TGG + chr1.1:541231-541250 None:intergenic 50.0%
GTCATTGCTAAGCTGAGGTG+AGG + chr1.1:541481-541500 None:intergenic 50.0%
GTCTCCTTGGTCAGGTACTA+GGG - chr1.1:541138-541157 MS.gene27083:CDS 50.0%
TCATGTGTAACCGGCGATTG+TGG - chr1.1:540093-540112 MS.gene27083:intron 50.0%
TCCGGAAAGGTTGAATGCTC+TGG - chr1.1:540117-540136 MS.gene27083:intron 50.0%
TGGAGAACAAAGCCTGTGGT+TGG + chr1.1:539979-539998 None:intergenic 50.0%
TGTGATAGGTCTCCTTGGTC+AGG - chr1.1:541130-541149 MS.gene27083:CDS 50.0%
! AAGCCTGTGGTTGGAAGTGT+TGG + chr1.1:539970-539989 None:intergenic 50.0%
! GCCAGAGCATTCAACCTTTC+CGG + chr1.1:540121-540140 None:intergenic 50.0%
! TCACATTAGACGGCTCTGGT+GGG - chr1.1:540175-540194 MS.gene27083:intron 50.0%
! TTCACATTAGACGGCTCTGG+TGG - chr1.1:540174-540193 MS.gene27083:intron 50.0%
!! AGCCTGTGGTTGGAAGTGTT+GGG + chr1.1:539969-539988 None:intergenic 50.0%
AGAGTTGGCACAACCGCCAA+AGG + chr1.1:541614-541633 None:intergenic 55.0%
ATGGGTGGCACACATGCCAA+GGG - chr1.1:541212-541231 MS.gene27083:CDS 55.0%
GGTCTCCTTGGTCAGGTACT+AGG - chr1.1:541137-541156 MS.gene27083:CDS 55.0%
GTCCCAACACTTCCAACCAC+AGG - chr1.1:539964-539983 MS.gene27083:intron 55.0%
GTGACTACACAGTGTGGCCA+GGG - chr1.1:539923-539942 MS.gene27083:intron 55.0%
TATGGGTGGCACACATGCCA+AGG - chr1.1:541211-541230 MS.gene27083:CDS 55.0%
TGGTCCCTAGTACCTGACCA+AGG + chr1.1:541145-541164 None:intergenic 55.0%
TGTGACTACACAGTGTGGCC+AGG - chr1.1:539922-539941 MS.gene27083:intron 55.0%
TTGGAGCACATGGGTGCACA+TGG + chr1.1:541553-541572 None:intergenic 55.0%
!! TCACCGGAGTATTGCTGCAG+CGG - chr1.1:540396-540415 MS.gene27083:intron 55.0%
ATTGCTGCAGCGGCGCGTAT+GGG - chr1.1:540406-540425 MS.gene27083:intron 60.0%
CCTATGCTAGTCGCCCCTCA+GGG - chr1.1:540240-540259 MS.gene27083:intron 60.0%
CTTTCCGGAGCCACAATCGC+CGG + chr1.1:540106-540125 None:intergenic 60.0%
GGCACACATGCCAAGGGCAA+GGG - chr1.1:541218-541237 MS.gene27083:CDS 60.0%
GGTGACAGTGGTGGTTTCGC+CGG + chr1.1:540004-540023 None:intergenic 60.0%
GTAACCGGCGATTGTGGCTC+CGG - chr1.1:540099-540118 MS.gene27083:intron 60.0%
TATTGCTGCAGCGGCGCGTA+TGG - chr1.1:540405-540424 MS.gene27083:intron 60.0%
TCTCAACGGCGCGTGTCCTT+CGG - chr1.1:540305-540324 MS.gene27083:intron 60.0%
TGGCACACATGCCAAGGGCA+AGG - chr1.1:541217-541236 MS.gene27083:CDS 60.0%
TGTCACCGCACCAGCCTCAT+GGG - chr1.1:540017-540036 MS.gene27083:intron 60.0%
! CAGTGGTGGTTTCGCCGGTT+TGG + chr1.1:539999-540018 None:intergenic 60.0%
ATGCTAGTCGCCCCTCAGGG+CGG - chr1.1:540243-540262 MS.gene27083:intron 65.0%
CCCTGAGGGGCGACTAGCAT+AGG + chr1.1:540243-540262 None:intergenic 65.0%
CGGCGATTGTGGCTCCGGAA+AGG - chr1.1:540104-540123 MS.gene27083:intron 65.0%
CTCATGGGGCGGCCGTTTCT+GGG - chr1.1:540032-540051 MS.gene27083:intron 65.0%
CTGTCACCGCACCAGCCTCA+TGG - chr1.1:540016-540035 MS.gene27083:intron 65.0%
GCCTATGCTAGTCGCCCCTC+AGG - chr1.1:540239-540258 MS.gene27083:intron 65.0%
GCGCCGCTGCAGCAATACTC+CGG + chr1.1:540402-540421 None:intergenic 65.0%
GGCTAAAGTTGCCGGCGGTG+TGG + chr1.1:540151-540170 None:intergenic 65.0%
GTCACCGCACCAGCCTCATG+GGG - chr1.1:540018-540037 MS.gene27083:intron 65.0%
TATCGCCAGAGCCGCCCTGA+GGG + chr1.1:540257-540276 None:intergenic 65.0%
TCATGGGGCGGCCGTTTCTG+GGG - chr1.1:540033-540052 MS.gene27083:intron 65.0%
TGAGGCTGGTGCGGTGACAG+TGG + chr1.1:540016-540035 None:intergenic 65.0%
TTATCGCCAGAGCCGCCCTG+AGG + chr1.1:540258-540277 None:intergenic 65.0%
AAACGGCCGCCCCATGAGGC+TGG + chr1.1:540030-540049 None:intergenic 70.0%
ACCGCACCAGCCTCATGGGG+CGG - chr1.1:540021-540040 MS.gene27083:intron 70.0%
ATCGCCAGAGCCGCCCTGAG+GGG + chr1.1:540256-540275 None:intergenic 70.0%
CCAGAAACGGCCGCCCCATG+AGG + chr1.1:540034-540053 None:intergenic 70.0%
CCTCATGGGGCGGCCGTTTC+TGG - chr1.1:540031-540050 MS.gene27083:intron 70.0%
GGCTGGTGCGGTGACAGTGG+TGG + chr1.1:540013-540032 None:intergenic 70.0%
TGGCAACGGAGCCACACCGC+CGG - chr1.1:540137-540156 MS.gene27083:intron 70.0%
GCCGCCCCATGAGGCTGGTG+CGG + chr1.1:540025-540044 None:intergenic 75.0%
! GTCGCCCCTCAGGGCGGCTC+TGG - chr1.1:540249-540268 MS.gene27083:intron 80.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 539691 541638 539691 ID=MS.gene27083
chr1.1 mRNA 539691 541638 539691 ID=MS.gene27083.t1;Parent=MS.gene27083
chr1.1 exon 541572 541638 541572 ID=MS.gene27083.t1.exon1;Parent=MS.gene27083.t1
chr1.1 CDS 541572 541638 541572 ID=cds.MS.gene27083.t1;Parent=MS.gene27083.t1
chr1.1 exon 540746 541442 540746 ID=MS.gene27083.t1.exon2;Parent=MS.gene27083.t1
chr1.1 CDS 540746 541442 540746 ID=cds.MS.gene27083.t1;Parent=MS.gene27083.t1
chr1.1 exon 539691 539904 539691 ID=MS.gene27083.t1.exon3;Parent=MS.gene27083.t1
chr1.1 CDS 539691 539904 539691 ID=cds.MS.gene27083.t1;Parent=MS.gene27083.t1
Gene Sequence

>MS.gene27083

ATGGATCACCACCACCAAGTACTATCAATTTTTTCATTCTTATTAGCACTTCATCTATCAATTTCAGGTAATTTTACAATTCAATTAAACAAAATTACCAAAAACAAACATTTAATAGTTCATAATATTTCGATAATATTTTGTTGACTAAATTTTCTCTTATATCTAATTAATTTTGTGAATATGTCATATGCAGGTGTAATTTCAACAACATTCACATTTGTTAACAAATGTGACTACACAGTGTGGCCAGGGATTCTTTCAAATGCTGGGGTCCCAACACTTCCAACCACAGGCTTTGTTCTCCAAACCGGCGAAACCACCACTGTCACCGCACCAGCCTCATGGGGCGGCCGTTTCTGGGGCAGAACTCTTTGCTCCCAAGACACTGATGGAAAATTCTCATGTGTAACCGGCGATTGTGGCTCCGGAAAGGTTGAATGCTCTGGCAACGGAGCCACACCGCCGGCAACTTTAGCCGAATTCACATTAGACGGCTCTGGTGGGCTTGACTTCTTCGATGTTAGTCTTGTTGACGGTTACAATGTGCCTATGCTAGTCGCCCCTCAGGGCGGCTCTGGCGATAATTGCACTACCACGGGATGTATCAGTGATCTCAACGGCGCGTGTCCTTCGGAGCTTAGAGTGATGAGTGTGGATGGGAAAGACGGTGTCGCGTGTAAGAGCGCATGCGAAGCTTTTAATTCACCGGAGTATTGCTGCAGCGGCGCGTATGGGACACCAGACACTTGCAAACCTTCTACTTATTCTCAATTGTTTAAGAACGCCTGCCCACGTGCATATAGCTATGCTTATGATGATAAGACCAGCACTTTCACTTGTGCAAATGCAGTAGATTATACTATCACATTTTGTTCTTCTCCAACCACAAGGTTAGTTACTTTATTTTTACTTCTCCTCAAAATAATAACCGTAAAAAATTTACCATTGTGCACCTACTGAATATAATCAATCTTACCGGTAAATCTTAACTAATATGATGATTCAACCCCTTATGAACCAATAGTTCGGAGTTTGATTCGATTAGATTGGGTAATGTATACAAGTGTTCTTTGTTTAGTAAAGTAGATCAAACCAAATGATTAATGAATTTTGTCAAATAATAAATTGTTCAATGAACTCGGGTTCGATTAATGGGTGAAATAATTTTTTATCCGTTATAATTTACCTTGCAACCGAACTCTAAATTACCTTACAGCAGAACAATTTTTCTATGAATCGGAGGGTTAATAGAACAAAAAAAGAATAACGATTTAATAGATGTTTGCTTTTTAGCCACCATGATTGGTGGGTAATCAATTTCAATAGAAATTGATGCAAAGGGAAACCATGTTTGCAATTGCAAGCAATCTAGTAGGTGAAGCAACATAATGGCCGGCAGAAATAGTTATGAATGTCACCAAATCCAAAATATCCAATGTGATAGGTCTCCTTGGTCAGGTACTAGGGACCATATCTAGAACCAATTATATTGATTGATGTACATTGGATTCGCAAAATATGGGTGGCACACATGCCAAGGGCAAGGGTTATCGTTACACAACACTATTGGTAATATGTCTACATCAAAATAAGTTACCAAAAAGTGTGATGAACTTTATGACCGTTAGGAAAATTACATTGTGACTCTTATTTTACATGTGATATTGTTATGTCTATTGTTGCATTTTTATTTTTTTTAGATTTTTGATCATTTTTCTATTGTATTTTCAGTCAAAAGGCAACACAAGGGCAAGACACAAAACAAGAAAGTTCTTCTAACTCTGCCTCACCTCAGCTTAGCAATGACAATACAATGGTGTATGAAGGTGGTATTGATCAAAGTGAAATTTCACAAGCCATGTGCACCCATGTGCTCCAATCACAAACTTTTGCGTGCATTATTAGCATCACTATGGTCCTTTGGCGGTTGTGCCAACTCTTCTAA
Protein sequence

>MS.gene27083.t1

MDHHHQVLSIFSFLLALHLSISGVISTTFTFVNKCDYTVWPGILSNAGVPTLPTTGFVLQTGETTTVTAPASWGGRFWGRTLCSQDTDGKFSCVTGDCGSGKVECSGNGATPPATLAEFTLDGSGGLDFFDVSLVDGYNVPMLVAPQGGSGDNCTTTGCISDLNGACPSELRVMSVDGKDGVACKSACEAFNSPEYCCSGAYGTPDTCKPSTYSQLFKNACPRAYSYAYDDKTSTFTCANAVDYTITFCSSPTTSQKATQGQDTKQESSSNSASPQLSNDNTMVYEGGIDQSEISQAMCTHVLQSQTFACIISITMVLWRLCQLF