AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64224

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64224.t1 MTR_1g025420 97.479 238 6 0 1 238 15 252 2.76e-171 474
MS.gene64224.t1 MTR_1g025420 98.312 237 4 0 1 237 15 251 6.09e-171 471
MS.gene64224.t1 MTR_3g114030 82.128 235 40 1 4 238 2 234 1.35e-142 399
MS.gene64224.t1 MTR_5g022310 70.339 236 66 3 5 237 22 256 7.53e-117 334
MS.gene64224.t1 MTR_5g022310 70.513 234 65 3 5 235 22 254 7.40e-116 333
MS.gene64224.t1 MTR_8g075550 70.464 237 63 3 2 233 21 255 1.19e-115 333
MS.gene64224.t1 MTR_8g037890 71.111 225 61 3 8 231 25 246 1.06e-114 331
MS.gene64224.t1 MTR_8g056820 69.298 228 66 3 5 231 22 246 2.70e-110 317
MS.gene64224.t1 MTR_4g073730 64.224 232 81 2 6 236 22 252 1.03e-107 312
MS.gene64224.t1 MTR_4g073730 64.224 232 81 2 6 236 22 252 3.49e-107 312
MS.gene64224.t1 MTR_4g073720 68.282 227 64 4 5 231 21 239 6.63e-103 302
MS.gene64224.t1 MTR_5g022350 62.656 241 76 7 1 238 21 250 1.32e-95 282
MS.gene64224.t1 MTR_8g075510 61.925 239 79 6 1 237 21 249 3.04e-95 279
MS.gene64224.t1 MTR_8g075510 62.661 233 75 6 1 231 21 243 1.99e-94 279
MS.gene64224.t1 MTR_7g102380 56.277 231 98 3 1 231 13 240 1.07e-92 272
MS.gene64224.t1 MTR_1g062590 56.757 222 93 3 10 231 23 241 3.37e-86 256
MS.gene64224.t1 MTR_1g062660 59.910 222 86 3 12 233 25 243 3.41e-86 256
MS.gene64224.t1 MTR_8g036215 56.828 227 93 5 5 231 16 237 6.70e-86 255
MS.gene64224.t1 MTR_1g062350 59.009 222 88 3 12 233 25 243 1.98e-85 254
MS.gene64224.t1 MTR_1g062370 58.559 222 89 3 12 233 25 243 2.30e-85 254
MS.gene64224.t1 MTR_2g068030 55.459 229 96 5 6 232 20 244 4.34e-85 253
MS.gene64224.t1 MTR_2g068655 54.386 228 100 3 5 231 27 251 1.21e-84 254
MS.gene64224.t1 MTR_2g069660 52.790 233 104 3 1 231 14 242 1.47e-84 252
MS.gene64224.t1 MTR_1g062630 57.207 222 92 3 12 233 25 243 3.76e-84 251
MS.gene64224.t1 MTR_1g062340 57.658 222 91 3 12 233 25 243 3.49e-83 248
MS.gene64224.t1 MTR_2g067980 56.957 230 91 5 6 231 21 246 1.62e-81 263
MS.gene64224.t1 MTR_8g107140 56.621 219 90 4 13 231 31 244 4.48e-80 241
MS.gene64224.t1 MTR_1g021945 57.522 226 88 6 8 231 25 244 5.27e-80 240
MS.gene64224.t1 MTR_6g079580 53.097 226 101 4 6 231 19 239 1.44e-78 236
MS.gene64224.t1 MTR_3g111620 54.018 224 97 6 9 231 27 245 6.80e-74 225
MS.gene64224.t1 MTR_1g062690 52.703 222 78 4 12 233 25 219 1.16e-69 213
MS.gene64224.t1 MTR_3g068015 50.222 225 105 7 9 231 28 247 5.97e-68 210
MS.gene64224.t1 MTR_5g059200 51.111 225 98 8 8 232 28 240 1.35e-66 207
MS.gene64224.t1 MTR_6g009480 45.374 227 118 6 8 231 28 251 4.08e-64 201
MS.gene64224.t1 MTR_7g062610 48.889 225 109 6 11 234 25 244 1.08e-63 199
MS.gene64224.t1 MTR_4g063630 43.668 229 121 7 8 232 28 252 9.24e-62 194
MS.gene64224.t1 MTR_5g023850 54.070 172 74 3 1 169 18 187 1.00e-58 185
MS.gene64224.t1 MTR_2g067990 47.368 228 90 8 5 231 23 221 3.01e-55 190
MS.gene64224.t1 MTR_2g067970 48.108 185 87 7 51 231 2 181 7.16e-50 174
MS.gene64224.t1 MTR_5g010640 40.506 237 114 7 2 231 19 235 6.22e-48 158
MS.gene64224.t1 MTR_5g022350 59.155 142 51 4 1 140 86 222 2.63e-47 156
MS.gene64224.t1 MTR_5g010635 40.000 225 111 7 8 231 23 224 1.05e-46 155
MS.gene64224.t1 MTR_8g096900 39.485 233 114 8 1 231 40 247 5.86e-45 151
MS.gene64224.t1 MTR_7g076360 38.356 219 70 4 3 221 2 155 1.58e-40 137
MS.gene64224.t1 MTR_8g089020 53.077 130 55 3 94 223 33 156 1.10e-38 132
MS.gene64224.t1 MTR_2g063150 33.036 224 140 6 3 225 30 244 8.33e-38 132
MS.gene64224.t1 MTR_1g062390 32.203 236 142 6 1 231 15 237 9.47e-33 119
MS.gene64224.t1 MTR_3g081550 57.447 94 40 0 94 187 16 109 2.18e-31 112
MS.gene64224.t1 MTR_8g096910 47.458 118 52 5 8 124 21 129 6.89e-26 99.8
MS.gene64224.t1 MTR_8g096920 43.220 118 57 4 11 127 21 129 2.96e-21 87.4
MS.gene64224.t1 MTR_1g062640 44.792 96 51 2 55 150 53 146 4.28e-18 78.6
MS.gene64224.t1 MTR_5g068670 43.750 80 42 2 47 126 13 89 3.84e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64224.t1 AT1G20030 79.915 234 45 1 5 236 16 249 6.09e-138 390
MS.gene64224.t1 AT1G20030 79.741 232 45 1 7 236 1 232 1.54e-136 385
MS.gene64224.t1 AT1G75800 75.949 237 55 1 4 238 18 254 2.24e-133 379
MS.gene64224.t1 AT4G36010 69.915 236 63 4 5 234 19 252 2.54e-114 329
MS.gene64224.t1 AT4G36010 69.915 236 63 4 5 234 19 252 2.54e-114 329
MS.gene64224.t1 AT4G38670 62.712 236 87 1 2 236 16 251 1.68e-106 309
MS.gene64224.t1 AT4G38670 62.712 236 87 1 2 236 16 251 1.68e-106 309
MS.gene64224.t1 AT4G38670 63.248 234 85 1 4 236 2 235 2.56e-106 308
MS.gene64224.t1 AT2G17860 63.793 232 79 4 8 235 22 252 4.20e-103 299
MS.gene64224.t1 AT4G38660 63.180 239 79 5 1 238 22 252 2.82e-99 293
MS.gene64224.t1 AT4G38660 64.255 235 75 5 5 238 4 230 5.99e-99 291
MS.gene64224.t1 AT4G24180 61.088 239 81 4 1 233 20 252 8.45e-95 278
MS.gene64224.t1 AT4G24180 61.088 239 81 4 1 233 25 257 1.18e-94 278
MS.gene64224.t1 AT4G24180 61.088 239 81 4 1 233 25 257 1.69e-94 280
MS.gene64224.t1 AT4G24180 61.088 239 81 4 1 233 20 252 2.20e-94 279
MS.gene64224.t1 AT5G38280 55.652 230 100 2 4 231 20 249 1.44e-91 283
MS.gene64224.t1 AT5G38280 55.652 230 100 2 4 231 20 249 2.32e-91 283
MS.gene64224.t1 AT4G24180 59.459 222 79 3 1 216 25 241 3.62e-86 259
MS.gene64224.t1 AT4G24180 59.459 222 79 3 1 216 20 236 3.69e-86 259
MS.gene64224.t1 AT1G19320 59.483 232 82 7 6 232 23 247 1.01e-85 255
MS.gene64224.t1 AT1G75030 58.667 225 87 6 8 231 23 242 4.70e-84 251
MS.gene64224.t1 AT1G75050 58.442 231 88 6 8 238 24 246 1.65e-83 249
MS.gene64224.t1 AT1G75040 58.850 226 85 6 6 231 21 238 4.55e-82 245
MS.gene64224.t1 AT1G18250 53.680 231 100 5 8 238 21 244 7.19e-81 243
MS.gene64224.t1 AT1G18250 53.680 231 100 5 8 238 20 243 8.47e-81 242
MS.gene64224.t1 AT1G73620 55.357 224 95 4 8 231 41 259 5.72e-80 241
MS.gene64224.t1 AT5G24620 52.766 235 104 5 8 237 24 256 3.25e-79 244
MS.gene64224.t1 AT5G24620 52.766 235 104 5 8 237 79 311 9.50e-79 244
MS.gene64224.t1 AT5G24620 52.766 235 104 5 8 237 100 332 1.24e-78 245
MS.gene64224.t1 AT1G77700 51.304 230 92 9 8 233 60 273 4.98e-70 218
MS.gene64224.t1 AT1G77700 51.327 226 98 8 8 233 60 273 1.02e-69 216
MS.gene64224.t1 AT1G77700 51.327 226 98 8 8 233 88 301 1.29e-69 218
MS.gene64224.t1 AT2G28790 45.798 238 119 7 1 232 16 249 3.60e-67 208
MS.gene64224.t1 AT2G28790 45.798 238 119 7 1 232 41 274 4.45e-67 208
MS.gene64224.t1 AT1G70250 47.458 236 111 6 7 236 143 371 5.40e-65 215
MS.gene64224.t1 AT4G36000 68.707 147 36 4 85 231 1 137 4.29e-64 198
MS.gene64224.t1 AT4G36000 68.707 147 36 4 85 231 1 137 4.29e-64 198
MS.gene64224.t1 AT5G40020 45.763 236 121 6 1 233 18 249 6.78e-64 199
MS.gene64224.t1 AT4G18253 43.162 234 123 4 4 236 18 242 1.04e-61 194
MS.gene64224.t1 AT5G02140 46.847 222 113 5 11 231 23 240 1.02e-60 191
MS.gene64224.t1 AT5G02140 46.847 222 113 5 11 231 23 240 1.34e-60 192
MS.gene64224.t1 AT4G18250 46.320 231 115 5 4 233 51 273 1.31e-57 194
MS.gene64224.t1 AT4G11650 40.260 231 111 9 8 237 22 226 6.01e-47 156
MS.gene64224.t1 AT5G24620 54.762 126 52 3 116 237 15 139 3.98e-38 135
MS.gene64224.t1 AT2G24810 62.195 82 26 3 152 232 116 193 7.69e-27 102

Find 78 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCATGGGGCGGCCGTTTC+TGG -0.083846 1.3:-1013536 MS.gene64224:CDS
GCCAGAGCATTCAACCTTTC+CGG 0.211647 1.3:+1013449 None:intergenic
CTCATGGGGCGGCCGTTTCT+GGG 0.253028 1.3:-1013535 MS.gene64224:CDS
CCAGGGATTCTTTCAAATGC+TGG 0.316773 1.3:-1013627 MS.gene64224:CDS
AACAATTGAGAATAAGTAGA+AGG 0.345262 1.3:+1013120 None:intergenic
CAGGGATTCTTTCAAATGCT+GGG 0.347178 1.3:-1013626 MS.gene64224:CDS
CAGTGGTGGTTTCGCCGGTT+TGG 0.373152 1.3:+1013571 None:intergenic
TATTGCTGCAGCGGCGCGTA+TGG 0.373787 1.3:-1013162 MS.gene64224:CDS
TTCGATGTTAGTCTTGTTGA+CGG 0.393312 1.3:-1013360 MS.gene64224:CDS
GGAAAATTCTCATGTGTAAC+CGG 0.404051 1.3:-1013483 MS.gene64224:CDS
GTCGCCCCTCAGGGCGGCTC+TGG 0.409338 1.3:-1013318 MS.gene64224:CDS
TCTCAACGGCGCGTGTCCTT+CGG 0.414761 1.3:-1013262 MS.gene64224:CDS
GCCTATGCTAGTCGCCCCTC+AGG 0.418663 1.3:-1013328 MS.gene64224:CDS
TCCGGAAAGGTTGAATGCTC+TGG 0.437194 1.3:-1013450 MS.gene64224:CDS
GTGAATTCGGCTAAAGTTGC+CGG 0.439691 1.3:+1013411 None:intergenic
AGAGCCGTCTAATGTGAATT+CGG 0.464663 1.3:+1013398 None:intergenic
TGTCACCGCACCAGCCTCAT+GGG 0.466682 1.3:-1013550 MS.gene64224:CDS
ACAGGCTTTGTTCTCCAAAC+CGG 0.470298 1.3:-1013585 MS.gene64224:CDS
GTAACCGGCGATTGTGGCTC+CGG 0.470556 1.3:-1013468 MS.gene64224:CDS
GTAGAAGGTTTGCAAGTGTC+TGG 0.470850 1.3:+1013135 None:intergenic
AAACGGCCGCCCCATGAGGC+TGG 0.484136 1.3:+1013540 None:intergenic
CTGTCACCGCACCAGCCTCA+TGG 0.485359 1.3:-1013551 MS.gene64224:CDS
CCAGCATTTGAAAGAATCCC+TGG 0.493036 1.3:+1013627 None:intergenic
TGTGACTACACAGTGTGGCC+AGG 0.496467 1.3:-1013645 MS.gene64224:CDS
GGTGACAGTGGTGGTTTCGC+CGG 0.497671 1.3:+1013566 None:intergenic
AAAGAGTTCTGCCCCAGAAA+CGG 0.509995 1.3:+1013523 None:intergenic
TCATGGGGCGGCCGTTTCTG+GGG 0.511165 1.3:-1013534 MS.gene64224:CDS
AAAGTAACTAACCTTGTGGT+TGG 0.518082 1.3:+1012994 None:intergenic
AAGCCTGTGGTTGGAAGTGT+TGG 0.519230 1.3:+1013600 None:intergenic
TATCGCCAGAGCCGCCCTGA+GGG 0.521997 1.3:+1013313 None:intergenic
CCCTGAGGGGCGACTAGCAT+AGG 0.525025 1.3:+1013327 None:intergenic
TCATGTGTAACCGGCGATTG+TGG 0.527231 1.3:-1013474 MS.gene64224:CDS
GAATTCACATTAGACGGCTC+TGG 0.528881 1.3:-1013396 MS.gene64224:CDS
CTTTGCTCCCAAGACACTGA+TGG 0.532188 1.3:-1013504 MS.gene64224:CDS
TTCACATTAGACGGCTCTGG+TGG 0.532251 1.3:-1013393 MS.gene64224:CDS
ATTGCTGCAGCGGCGCGTAT+GGG 0.534091 1.3:-1013161 MS.gene64224:CDS
GGCGATAATTGCACTACCAC+GGG 0.537099 1.3:-1013297 MS.gene64224:CDS
GGCTAAAGTTGCCGGCGGTG+TGG 0.544415 1.3:+1013419 None:intergenic
ATAGCTATATGCACGTGGGC+AGG 0.548259 1.3:+1013089 None:intergenic
CTTAGAGTGATGAGTGTGGA+TGG 0.554333 1.3:-1013237 MS.gene64224:CDS
TGGAGAACAAAGCCTGTGGT+TGG 0.555388 1.3:+1013591 None:intergenic
CCTATGCTAGTCGCCCCTCA+GGG 0.555587 1.3:-1013327 MS.gene64224:CDS
AGGGATTCTTTCAAATGCTG+GGG 0.560190 1.3:-1013625 MS.gene64224:CDS
GCCGCCCCATGAGGCTGGTG+CGG 0.562230 1.3:+1013545 None:intergenic
GCGCCGCTGCAGCAATACTC+CGG 0.562265 1.3:+1013168 None:intergenic
CTTTCCGGAGCCACAATCGC+CGG 0.563498 1.3:+1013464 None:intergenic
TTTGTGAATATGTCATATGC+AGG 0.565168 1.3:-1013702 None:intergenic
CGGCGATTGTGGCTCCGGAA+AGG 0.566510 1.3:-1013463 MS.gene64224:CDS
GTGACTACACAGTGTGGCCA+GGG 0.569936 1.3:-1013644 MS.gene64224:CDS
GTCCCAACACTTCCAACCAC+AGG 0.572652 1.3:-1013603 MS.gene64224:CDS
AAGGTTGAATGCTCTGGCAA+CGG 0.576244 1.3:-1013444 MS.gene64224:CDS
TTAGCCGAATTCACATTAGA+CGG 0.576825 1.3:-1013402 MS.gene64224:CDS
TGGCAACGGAGCCACACCGC+CGG 0.578628 1.3:-1013430 MS.gene64224:CDS
ACCGCACCAGCCTCATGGGG+CGG 0.586044 1.3:-1013546 MS.gene64224:CDS
ATGAGTGTGGATGGGAAAGA+CGG 0.592613 1.3:-1013228 MS.gene64224:CDS
TGGCGATAATTGCACTACCA+CGG 0.593427 1.3:-1013298 MS.gene64224:CDS
CCAGAAACGGCCGCCCCATG+AGG 0.596649 1.3:+1013536 None:intergenic
TCACCGGAGTATTGCTGCAG+CGG 0.602026 1.3:-1013171 MS.gene64224:CDS
ATTTGCACAAGTGAAAGTGC+TGG 0.609107 1.3:+1013050 None:intergenic
TTATCGCCAGAGCCGCCCTG+AGG 0.617214 1.3:+1013312 None:intergenic
AGCCTGTGGTTGGAAGTGTT+GGG 0.621679 1.3:+1013601 None:intergenic
TCACATTAGACGGCTCTGGT+GGG 0.623574 1.3:-1013392 MS.gene64224:CDS
TGAGGCTGGTGCGGTGACAG+TGG 0.623668 1.3:+1013554 None:intergenic
ATCGCCAGAGCCGCCCTGAG+GGG 0.629555 1.3:+1013314 None:intergenic
TTAGAGTGATGAGTGTGGAT+GGG 0.630518 1.3:-1013236 MS.gene64224:CDS
GTCACCGCACCAGCCTCATG+GGG 0.632870 1.3:-1013549 MS.gene64224:CDS
GGCTGGTGCGGTGACAGTGG+TGG 0.635446 1.3:+1013557 None:intergenic
TTTGTTCTTCTCCAACCACA+AGG 0.638050 1.3:-1013005 MS.gene64224:intron
CTCATCACTCTAAGCTCCGA+AGG 0.638536 1.3:+1013246 None:intergenic
TAAGCATAGCTATATGCACG+TGG 0.665332 1.3:+1013084 None:intergenic
GGATGTATCAGTGATCTCAA+CGG 0.673930 1.3:-1013276 MS.gene64224:CDS
GAGATCACTGATACATCCCG+TGG 0.679790 1.3:+1013281 None:intergenic
ATGCTAGTCGCCCCTCAGGG+CGG 0.689357 1.3:-1013324 MS.gene64224:CDS
GGTTTGGAGAACAAAGCCTG+TGG 0.698538 1.3:+1013587 None:intergenic
AATTCGGCTAAAGTTGCCGG+CGG 0.706419 1.3:+1013414 None:intergenic
AAGCATAGCTATATGCACGT+GGG 0.721806 1.3:+1013085 None:intergenic
GGAGCTTAGAGTGATGAGTG+TGG 0.722376 1.3:-1013241 MS.gene64224:CDS
ACAAATGTGACTACACAGTG+TGG 0.729139 1.3:-1013650 MS.gene64224:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGATTGATTATATTCAAT+AGG + chr1.3:1013338-1013357 None:intergenic 15.0%
!! AATAAATTGTTCAATGAACT+CGG - chr1.3:1013502-1013521 MS.gene64224:CDS 20.0%
!! GACAAAATTCATTAATCATT+TGG + chr1.3:1013478-1013497 None:intergenic 20.0%
!! TCATATTAGTTAAGATTTAC+CGG + chr1.3:1013361-1013380 None:intergenic 20.0%
!! TTGAATATAATCAATCTTAC+CGG - chr1.3:1013339-1013358 MS.gene64224:CDS 20.0%
!!! AGAACAATTTTTCTATGAAT+CGG - chr1.3:1013600-1013619 MS.gene64224:CDS 20.0%
!!! TTTTTACGGTTATTATTTTG+AGG + chr1.3:1013300-1013319 None:intergenic 20.0%
! AAATAAAGTAACTAACCTTG+TGG + chr1.3:1013269-1013288 None:intergenic 25.0%
! AACAATTGAGAATAAGTAGA+AGG + chr1.3:1013139-1013158 None:intergenic 25.0%
! ATAAATTGTTCAATGAACTC+GGG - chr1.3:1013503-1013522 MS.gene64224:CDS 25.0%
!!! GCACAATGGTAAATTTTTTA+CGG + chr1.3:1013314-1013333 None:intergenic 25.0%
AGAAAAATTGTTCTGCTGTA+AGG + chr1.3:1013594-1013613 None:intergenic 30.0%
TATATTCAATAGGTGCACAA+TGG + chr1.3:1013328-1013347 None:intergenic 30.0%
! ACAATTTTTCTATGAATCGG+AGG - chr1.3:1013603-1013622 MS.gene64224:CDS 30.0%
! CAATTTTTCTATGAATCGGA+GGG - chr1.3:1013604-1013623 MS.gene64224:CDS 30.0%
! CTGTAAGGTAATTTAGAGTT+CGG + chr1.3:1013579-1013598 None:intergenic 30.0%
! GAGTTTGATTCGATTAGATT+GGG - chr1.3:1013411-1013430 MS.gene64224:CDS 30.0%
AAAGTAACTAACCTTGTGGT+TGG + chr1.3:1013265-1013284 None:intergenic 35.0%
AATTGATTACCCACCAATCA+TGG + chr1.3:1013682-1013701 None:intergenic 35.0%
GGAAAATTCTCATGTGTAAC+CGG - chr1.3:1012773-1012792 MS.gene64224:intron 35.0%
GGTCGCAAGGTAAATTATAA+CGG + chr1.3:1013558-1013577 None:intergenic 35.0%
TTAGCCGAATTCACATTAGA+CGG - chr1.3:1012854-1012873 MS.gene64224:intron 35.0%
! AGAATTTTCCATCAGTGTCT+TGG + chr1.3:1012763-1012782 None:intergenic 35.0%
! GAATTTTCCATCAGTGTCTT+GGG + chr1.3:1012762-1012781 None:intergenic 35.0%
! GGAGTTTGATTCGATTAGAT+TGG - chr1.3:1013410-1013429 MS.gene64224:CDS 35.0%
!! ATGCGAAGCTTTTAATTCAC+CGG - chr1.3:1013069-1013088 MS.gene64224:CDS 35.0%
!! TCCGAACTATTGGTTCATAA+GGG + chr1.3:1013393-1013412 None:intergenic 35.0%
!! TTCGATGTTAGTCTTGTTGA+CGG - chr1.3:1012896-1012915 MS.gene64224:intron 35.0%
AAGCATAGCTATATGCACGT+GGG + chr1.3:1013174-1013193 None:intergenic 40.0%
ACAAATGTGACTACACAGTG+TGG - chr1.3:1012606-1012625 MS.gene64224:intron 40.0%
AGAGCCGTCTAATGTGAATT+CGG + chr1.3:1012861-1012880 None:intergenic 40.0%
AGGGATTCTTTCAAATGCTG+GGG - chr1.3:1012631-1012650 MS.gene64224:intron 40.0%
ATGAACTCGGGTTCGATTAA+TGG - chr1.3:1013515-1013534 MS.gene64224:CDS 40.0%
CAGGGATTCTTTCAAATGCT+GGG - chr1.3:1012630-1012649 MS.gene64224:intron 40.0%
CCCCTTATGAACCAATAGTT+CGG - chr1.3:1013389-1013408 MS.gene64224:CDS 40.0%
CGAATCAAACTCCGAACTAT+TGG + chr1.3:1013403-1013422 None:intergenic 40.0%
GGATGTATCAGTGATCTCAA+CGG - chr1.3:1012980-1012999 MS.gene64224:intron 40.0%
TAAGCATAGCTATATGCACG+TGG + chr1.3:1013175-1013194 None:intergenic 40.0%
TGAACTCGGGTTCGATTAAT+GGG - chr1.3:1013516-1013535 MS.gene64224:CDS 40.0%
TTAGAGTGATGAGTGTGGAT+GGG - chr1.3:1013020-1013039 MS.gene64224:CDS 40.0%
TTTGTTCTTCTCCAACCACA+AGG - chr1.3:1013251-1013270 MS.gene64224:CDS 40.0%
! ATTTGCACAAGTGAAAGTGC+TGG + chr1.3:1013209-1013228 None:intergenic 40.0%
! TAATTTAGAGTTCGGTCGCA+AGG + chr1.3:1013571-1013590 None:intergenic 40.0%
! TTTTAGCCACCATGATTGGT+GGG - chr1.3:1013670-1013689 MS.gene64224:CDS 40.0%
!! CCGAACTATTGGTTCATAAG+GGG + chr1.3:1013392-1013411 None:intergenic 40.0%
!! CTCCGAACTATTGGTTCATA+AGG + chr1.3:1013394-1013413 None:intergenic 40.0%
!! TGCTTTTTAGCCACCATGAT+TGG - chr1.3:1013666-1013685 MS.gene64224:CDS 40.0%
!! TTTTTAGCCACCATGATTGG+TGG - chr1.3:1013669-1013688 MS.gene64224:CDS 40.0%
AAAGAGTTCTGCCCCAGAAA+CGG + chr1.3:1012736-1012755 None:intergenic 45.0%
AAGGTTGAATGCTCTGGCAA+CGG - chr1.3:1012812-1012831 MS.gene64224:intron 45.0%
ATGAGTGTGGATGGGAAAGA+CGG - chr1.3:1013028-1013047 MS.gene64224:CDS 45.0%
CCAGCATTTGAAAGAATCCC+TGG + chr1.3:1012632-1012651 None:intergenic 45.0%
CCAGGGATTCTTTCAAATGC+TGG - chr1.3:1012629-1012648 MS.gene64224:intron 45.0%
CTTAGAGTGATGAGTGTGGA+TGG - chr1.3:1013019-1013038 MS.gene64224:CDS 45.0%
GAATTCACATTAGACGGCTC+TGG - chr1.3:1012860-1012879 MS.gene64224:intron 45.0%
GTAGAAGGTTTGCAAGTGTC+TGG + chr1.3:1013124-1013143 None:intergenic 45.0%
GTGAATTCGGCTAAAGTTGC+CGG + chr1.3:1012848-1012867 None:intergenic 45.0%
TGATTACCCACCAATCATGG+TGG + chr1.3:1013679-1013698 None:intergenic 45.0%
TGGCGATAATTGCACTACCA+CGG - chr1.3:1012958-1012977 MS.gene64224:intron 45.0%
!! ACAGGCTTTGTTCTCCAAAC+CGG - chr1.3:1012671-1012690 MS.gene64224:intron 45.0%
AATTCGGCTAAAGTTGCCGG+CGG + chr1.3:1012845-1012864 None:intergenic 50.0%
ATAGCTATATGCACGTGGGC+AGG + chr1.3:1013170-1013189 None:intergenic 50.0%
CTCATCACTCTAAGCTCCGA+AGG + chr1.3:1013013-1013032 None:intergenic 50.0%
CTTTGCTCCCAAGACACTGA+TGG - chr1.3:1012752-1012771 MS.gene64224:intron 50.0%
GAGATCACTGATACATCCCG+TGG + chr1.3:1012978-1012997 None:intergenic 50.0%
GGAGCTTAGAGTGATGAGTG+TGG - chr1.3:1013015-1013034 MS.gene64224:CDS 50.0%
GGCGATAATTGCACTACCAC+GGG - chr1.3:1012959-1012978 MS.gene64224:intron 50.0%
GGTTTGGAGAACAAAGCCTG+TGG + chr1.3:1012672-1012691 None:intergenic 50.0%
TCATGTGTAACCGGCGATTG+TGG - chr1.3:1012782-1012801 MS.gene64224:intron 50.0%
TCCGGAAAGGTTGAATGCTC+TGG - chr1.3:1012806-1012825 MS.gene64224:intron 50.0%
TGGAGAACAAAGCCTGTGGT+TGG + chr1.3:1012668-1012687 None:intergenic 50.0%
! AAGCCTGTGGTTGGAAGTGT+TGG + chr1.3:1012659-1012678 None:intergenic 50.0%
! GCCAGAGCATTCAACCTTTC+CGG + chr1.3:1012810-1012829 None:intergenic 50.0%
! TCACATTAGACGGCTCTGGT+GGG - chr1.3:1012864-1012883 MS.gene64224:intron 50.0%
! TTCACATTAGACGGCTCTGG+TGG - chr1.3:1012863-1012882 MS.gene64224:intron 50.0%
!! AGCCTGTGGTTGGAAGTGTT+GGG + chr1.3:1012658-1012677 None:intergenic 50.0%
GTCCCAACACTTCCAACCAC+AGG - chr1.3:1012653-1012672 MS.gene64224:intron 55.0%
GTGACTACACAGTGTGGCCA+GGG - chr1.3:1012612-1012631 MS.gene64224:intron 55.0%
TGTGACTACACAGTGTGGCC+AGG - chr1.3:1012611-1012630 MS.gene64224:intron 55.0%
!! TCACCGGAGTATTGCTGCAG+CGG - chr1.3:1013085-1013104 MS.gene64224:CDS 55.0%
ATTGCTGCAGCGGCGCGTAT+GGG - chr1.3:1013095-1013114 MS.gene64224:CDS 60.0%
CCTATGCTAGTCGCCCCTCA+GGG - chr1.3:1012929-1012948 MS.gene64224:intron 60.0%
CTTTCCGGAGCCACAATCGC+CGG + chr1.3:1012795-1012814 None:intergenic 60.0%
GGTGACAGTGGTGGTTTCGC+CGG + chr1.3:1012693-1012712 None:intergenic 60.0%
GTAACCGGCGATTGTGGCTC+CGG - chr1.3:1012788-1012807 MS.gene64224:intron 60.0%
TATTGCTGCAGCGGCGCGTA+TGG - chr1.3:1013094-1013113 MS.gene64224:CDS 60.0%
TCTCAACGGCGCGTGTCCTT+CGG - chr1.3:1012994-1013013 MS.gene64224:intron 60.0%
TGTCACCGCACCAGCCTCAT+GGG - chr1.3:1012706-1012725 MS.gene64224:intron 60.0%
! CAGTGGTGGTTTCGCCGGTT+TGG + chr1.3:1012688-1012707 None:intergenic 60.0%
ATGCTAGTCGCCCCTCAGGG+CGG - chr1.3:1012932-1012951 MS.gene64224:intron 65.0%
CCCTGAGGGGCGACTAGCAT+AGG + chr1.3:1012932-1012951 None:intergenic 65.0%
CGGCGATTGTGGCTCCGGAA+AGG - chr1.3:1012793-1012812 MS.gene64224:intron 65.0%
CTCATGGGGCGGCCGTTTCT+GGG - chr1.3:1012721-1012740 MS.gene64224:intron 65.0%
CTGTCACCGCACCAGCCTCA+TGG - chr1.3:1012705-1012724 MS.gene64224:intron 65.0%
GCCTATGCTAGTCGCCCCTC+AGG - chr1.3:1012928-1012947 MS.gene64224:intron 65.0%
GCGCCGCTGCAGCAATACTC+CGG + chr1.3:1013091-1013110 None:intergenic 65.0%
GGCTAAAGTTGCCGGCGGTG+TGG + chr1.3:1012840-1012859 None:intergenic 65.0%
GTCACCGCACCAGCCTCATG+GGG - chr1.3:1012707-1012726 MS.gene64224:intron 65.0%
TATCGCCAGAGCCGCCCTGA+GGG + chr1.3:1012946-1012965 None:intergenic 65.0%
TCATGGGGCGGCCGTTTCTG+GGG - chr1.3:1012722-1012741 MS.gene64224:intron 65.0%
TGAGGCTGGTGCGGTGACAG+TGG + chr1.3:1012705-1012724 None:intergenic 65.0%
TTATCGCCAGAGCCGCCCTG+AGG + chr1.3:1012947-1012966 None:intergenic 65.0%
AAACGGCCGCCCCATGAGGC+TGG + chr1.3:1012719-1012738 None:intergenic 70.0%
ACCGCACCAGCCTCATGGGG+CGG - chr1.3:1012710-1012729 MS.gene64224:intron 70.0%
ATCGCCAGAGCCGCCCTGAG+GGG + chr1.3:1012945-1012964 None:intergenic 70.0%
CCAGAAACGGCCGCCCCATG+AGG + chr1.3:1012723-1012742 None:intergenic 70.0%
CCTCATGGGGCGGCCGTTTC+TGG - chr1.3:1012720-1012739 MS.gene64224:intron 70.0%
GGCTGGTGCGGTGACAGTGG+TGG + chr1.3:1012702-1012721 None:intergenic 70.0%
TGGCAACGGAGCCACACCGC+CGG - chr1.3:1012826-1012845 MS.gene64224:intron 70.0%
GCCGCCCCATGAGGCTGGTG+CGG + chr1.3:1012714-1012733 None:intergenic 75.0%
! GTCGCCCCTCAGGGCGGCTC+TGG - chr1.3:1012938-1012957 MS.gene64224:intron 80.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 1012563 1013715 1012563 ID=MS.gene64224
chr1.3 mRNA 1012563 1013715 1012563 ID=MS.gene64224.t1;Parent=MS.gene64224
chr1.3 exon 1013006 1013715 1013006 ID=MS.gene64224.t1.exon1;Parent=MS.gene64224.t1
chr1.3 CDS 1013006 1013715 1013006 ID=cds.MS.gene64224.t1;Parent=MS.gene64224.t1
chr1.3 exon 1012563 1012569 1012563 ID=MS.gene64224.t1.exon2;Parent=MS.gene64224.t1
chr1.3 CDS 1012563 1012569 1012563 ID=cds.MS.gene64224.t1;Parent=MS.gene64224.t1
Gene Sequence

>MS.gene64224

ATGTCATATGCAGGTGTAATTTCAACAACATTCACATTTGTTAACAAATGTGACTACACAGTGTGGCCAGGGATTCTTTCAAATGCTGGGGTCCCAACACTTCCAACCACAGGCTTTGTTCTCCAAACCGGCGAAACCACCACTGTCACCGCACCAGCCTCATGGGGCGGCCGTTTCTGGGGCAGAACTCTTTGCTCCCAAGACACTGATGGAAAATTCTCATGTGTAACCGGCGATTGTGGCTCCGGAAAGGTTGAATGCTCTGGCAACGGAGCCACACCGCCGGCAACTTTAGCCGAATTCACATTAGACGGCTCTGGTGGGCTTGACTTCTTCGATGTTAGTCTTGTTGACGGTTACAATGTGCCTATGCTAGTCGCCCCTCAGGGCGGCTCTGGCGATAATTGCACTACCACGGGATGTATCAGTGATCTCAACGGCGCGTGTCCTTCGGAGCTTAGAGTGATGAGTGTGGATGGGAAAGACGGTGTCGCGTGTAAGAGCGCATGCGAAGCTTTTAATTCACCGGAGTATTGCTGCAGCGGCGCGTATGGGACACCAGACACTTGCAAACCTTCTACTTATTCTCAATTGTTTAAGAACGCCTGCCCACGTGCATATAGCTATGCTTATGATGATAAGACCAGCACTTTCACTTGTGCAAATGCAGTAGATTATACTATCACATTTTGTTCTTCTCCAACCACAAGGTTAGTTACTTTATTTTTACTTCTCCTCAAAATAATAACCGTAAAAAATTTACCATTGTGCACCTATTGAATATAATCAATCTTACCGGTAAATCTTAACTAATATGATGATTCAACCCCTTATGAACCAATAGTTCGGAGTTTGATTCGATTAGATTGGGTAATGTATACAAGTGTTCTTTGTTTAGTAAAGTAGATCAAACCAAATGATTAATGAATTTTGTCAAATAATAAATTGTTCAATGAACTCGGGTTCGATTAATGGGTGAAACAATTTTTTATCCGTTATAATTTACCTTGCGACCGAACTCTAAATTACCTTACAGCAGAACAATTTTTCTATGAATCGGAGGGTTAATAGAACAAAAAAAGAATAACGATTTAATAGATGTTTGCTTTTTAGCCACCATGATTGGTGGGTAATCAATTTCAATAGAAATTGA
Protein sequence

>MS.gene64224.t1

MSYAGVISTTFTFVNKCDYTVWPGILSNAGVPTLPTTGFVLQTGETTTVTAPASWGGRFWGRTLCSQDTDGKFSCVTGDCGSGKVECSGNGATPPATLAEFTLDGSGGLDFFDVSLVDGYNVPMLVAPQGGSGDNCTTTGCISDLNGACPSELRVMSVDGKDGVACKSACEAFNSPEYCCSGAYGTPDTCKPSTYSQLFKNACPRAYSYAYDDKTSTFTCANAVDYTITFCSSPTTRN