AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64022

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64022.t1 MTR_1g025420 96.117 309 10 1 1 307 15 323 0.0 605
MS.gene64022.t1 MTR_1g025420 97.881 236 5 0 1 236 15 250 4.18e-170 472
MS.gene64022.t1 MTR_3g114030 80.932 236 45 0 4 239 2 237 2.07e-142 401
MS.gene64022.t1 MTR_8g037890 59.532 299 104 8 8 300 25 312 8.92e-117 339
MS.gene64022.t1 MTR_5g022310 69.915 236 67 3 5 237 22 256 5.79e-116 336
MS.gene64022.t1 MTR_5g022310 70.085 234 66 3 5 235 22 254 1.78e-115 333
MS.gene64022.t1 MTR_8g075550 70.042 237 64 3 2 233 21 255 2.87e-115 335
MS.gene64022.t1 MTR_4g073730 54.333 300 124 4 6 304 22 309 2.48e-112 328
MS.gene64022.t1 MTR_8g056820 67.364 239 72 4 5 242 22 255 1.16e-110 321
MS.gene64022.t1 MTR_4g073730 59.316 263 97 3 6 267 22 275 5.57e-110 321
MS.gene64022.t1 MTR_4g073720 67.841 227 65 4 5 231 21 239 1.72e-102 304
MS.gene64022.t1 MTR_5g022350 52.318 302 131 6 1 300 21 311 1.07e-97 290
MS.gene64022.t1 MTR_8g075510 62.553 235 76 6 1 233 21 245 6.00e-95 281
MS.gene64022.t1 MTR_8g075510 62.661 233 75 6 1 231 21 243 9.37e-95 283
MS.gene64022.t1 MTR_7g102380 56.277 231 98 3 1 231 13 240 3.09e-92 274
MS.gene64022.t1 MTR_1g062660 58.009 231 94 3 3 233 16 243 4.84e-86 258
MS.gene64022.t1 MTR_8g036215 56.828 227 93 5 5 231 16 237 1.18e-85 257
MS.gene64022.t1 MTR_1g062590 56.757 222 93 3 10 231 23 241 1.18e-85 257
MS.gene64022.t1 MTR_1g062350 59.009 222 88 3 12 233 25 243 4.93e-85 256
MS.gene64022.t1 MTR_1g062370 58.559 222 89 3 12 233 25 243 5.80e-85 256
MS.gene64022.t1 MTR_2g068030 55.702 228 95 5 6 231 20 243 1.33e-84 255
MS.gene64022.t1 MTR_2g068655 54.386 228 100 3 5 231 27 251 3.48e-84 256
MS.gene64022.t1 MTR_1g062630 57.207 222 92 3 12 233 25 243 4.96e-84 254
MS.gene64022.t1 MTR_2g069660 53.478 230 101 3 4 231 17 242 5.83e-84 253
MS.gene64022.t1 MTR_1g062340 57.658 222 91 3 12 233 25 243 7.45e-83 250
MS.gene64022.t1 MTR_2g067980 54.508 244 101 6 6 245 21 258 1.67e-81 266
MS.gene64022.t1 MTR_1g021945 57.522 226 88 6 8 231 25 244 6.27e-80 243
MS.gene64022.t1 MTR_8g107140 56.621 219 90 4 13 231 31 244 7.88e-80 243
MS.gene64022.t1 MTR_6g079580 53.097 226 101 4 6 231 19 239 4.46e-78 238
MS.gene64022.t1 MTR_3g111620 53.913 230 98 7 9 237 27 249 2.43e-73 226
MS.gene64022.t1 MTR_1g062690 52.703 222 78 4 12 233 25 219 2.29e-69 215
MS.gene64022.t1 MTR_3g068015 50.222 225 105 7 9 231 28 247 6.55e-67 209
MS.gene64022.t1 MTR_5g059200 51.111 225 98 8 8 232 28 240 1.50e-66 210
MS.gene64022.t1 MTR_6g009480 45.778 225 116 6 10 231 30 251 1.62e-63 202
MS.gene64022.t1 MTR_7g062610 48.889 225 109 6 11 234 25 244 8.21e-63 199
MS.gene64022.t1 MTR_4g063630 44.248 226 118 7 11 232 31 252 4.53e-61 195
MS.gene64022.t1 MTR_5g023850 53.488 172 75 3 1 169 18 187 9.64e-58 185
MS.gene64022.t1 MTR_2g067990 47.391 230 87 9 5 231 23 221 6.89e-55 192
MS.gene64022.t1 MTR_2g067970 46.193 197 95 8 51 243 2 191 2.60e-49 176
MS.gene64022.t1 MTR_5g010640 40.084 237 115 7 2 231 19 235 4.45e-47 158
MS.gene64022.t1 MTR_5g022350 59.155 142 51 4 1 140 86 222 6.50e-47 157
MS.gene64022.t1 MTR_5g010635 40.000 225 111 7 8 231 23 224 5.14e-46 155
MS.gene64022.t1 MTR_8g096900 39.485 233 114 8 1 231 40 247 1.20e-44 152
MS.gene64022.t1 MTR_7g076360 38.356 219 70 4 3 221 2 155 4.97e-40 138
MS.gene64022.t1 MTR_8g089020 53.077 130 55 3 94 223 33 156 2.81e-38 133
MS.gene64022.t1 MTR_2g063150 33.036 224 140 6 3 225 30 244 5.22e-37 133
MS.gene64022.t1 MTR_1g062390 31.513 238 145 6 1 233 15 239 2.00e-31 118
MS.gene64022.t1 MTR_3g081550 57.447 94 40 0 94 187 16 109 8.77e-31 112
MS.gene64022.t1 MTR_8g096910 47.458 118 52 5 8 124 21 129 1.76e-25 100
MS.gene64022.t1 MTR_8g096920 42.149 121 60 4 8 127 18 129 9.84e-21 87.8
MS.gene64022.t1 MTR_1g062640 43.750 96 52 2 55 150 53 146 2.38e-17 78.2
MS.gene64022.t1 MTR_3g114040 56.944 72 27 2 237 307 33 101 2.62e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64022.t1 AT1G20030 66.230 305 97 3 5 307 16 316 2.89e-143 406
MS.gene64022.t1 AT1G75800 63.863 321 86 5 4 303 18 329 7.30e-143 406
MS.gene64022.t1 AT1G20030 66.007 303 97 3 7 307 1 299 8.61e-142 402
MS.gene64022.t1 AT4G36010 63.370 273 92 4 5 271 19 289 8.14e-117 338
MS.gene64022.t1 AT4G36010 63.370 273 92 4 5 271 19 289 8.14e-117 338
MS.gene64022.t1 AT4G38670 62.343 239 89 1 2 239 16 254 6.81e-108 315
MS.gene64022.t1 AT4G38670 62.343 239 89 1 2 239 16 254 6.81e-108 315
MS.gene64022.t1 AT4G38670 62.869 237 87 1 4 239 2 238 8.40e-108 314
MS.gene64022.t1 AT2G17860 63.362 232 80 4 8 235 22 252 1.29e-102 301
MS.gene64022.t1 AT4G38660 61.417 254 89 5 1 254 22 266 4.50e-100 298
MS.gene64022.t1 AT4G38660 62.400 250 85 5 5 254 4 244 9.51e-100 296
MS.gene64022.t1 AT4G24180 57.414 263 100 4 1 257 20 276 2.23e-95 285
MS.gene64022.t1 AT4G24180 57.414 263 100 4 1 257 25 281 2.80e-95 284
MS.gene64022.t1 AT4G24180 60.669 239 82 4 1 233 20 252 3.95e-94 279
MS.gene64022.t1 AT4G24180 60.669 239 82 4 1 233 25 257 4.00e-94 280
MS.gene64022.t1 AT5G38280 53.937 254 110 4 4 251 20 272 8.11e-93 289
MS.gene64022.t1 AT5G38280 53.937 254 110 4 4 251 20 272 1.64e-92 288
MS.gene64022.t1 AT4G24180 59.009 222 80 3 1 216 20 236 1.71e-85 259
MS.gene64022.t1 AT1G19320 59.483 232 82 7 6 232 23 247 2.12e-85 257
MS.gene64022.t1 AT4G24180 59.009 222 80 3 1 216 25 241 2.21e-85 259
MS.gene64022.t1 AT1G75030 58.667 225 87 6 8 231 23 242 1.02e-83 253
MS.gene64022.t1 AT1G75050 58.929 224 87 5 8 231 24 242 1.00e-82 250
MS.gene64022.t1 AT1G75040 58.850 226 85 6 6 231 21 238 8.93e-82 247
MS.gene64022.t1 AT1G18250 54.464 224 97 4 8 231 21 239 3.04e-80 243
MS.gene64022.t1 AT1G18250 54.464 224 97 4 8 231 20 238 3.65e-80 243
MS.gene64022.t1 AT1G73620 55.357 224 95 4 8 231 41 259 1.24e-79 243
MS.gene64022.t1 AT5G24620 53.275 229 100 5 8 231 24 250 2.51e-78 244
MS.gene64022.t1 AT5G24620 53.275 229 100 5 8 231 79 305 4.50e-78 246
MS.gene64022.t1 AT5G24620 53.275 229 100 5 8 231 100 326 5.71e-78 246
MS.gene64022.t1 AT1G77700 45.614 285 134 10 8 288 60 327 4.89e-70 220
MS.gene64022.t1 AT1G77700 51.327 226 98 8 8 233 60 273 1.96e-69 218
MS.gene64022.t1 AT1G77700 45.552 281 140 9 8 288 88 355 2.92e-69 219
MS.gene64022.t1 AT2G28790 45.570 237 119 7 1 231 16 248 2.93e-66 208
MS.gene64022.t1 AT2G28790 45.570 237 119 7 1 231 41 273 3.36e-66 209
MS.gene64022.t1 AT1G70250 47.083 240 114 6 7 240 143 375 3.81e-65 219
MS.gene64022.t1 AT4G36000 62.069 174 54 5 85 256 1 164 4.15e-64 201
MS.gene64022.t1 AT4G36000 62.069 174 54 5 85 256 1 164 4.15e-64 201
MS.gene64022.t1 AT5G40020 45.339 236 122 6 1 233 18 249 4.45e-63 200
MS.gene64022.t1 AT5G02140 45.064 233 123 5 11 242 23 251 5.18e-61 196
MS.gene64022.t1 AT4G18253 42.553 235 125 4 4 237 18 243 1.93e-60 193
MS.gene64022.t1 AT5G02140 46.847 222 113 5 11 231 23 240 3.98e-60 192
MS.gene64022.t1 AT4G18250 42.857 259 137 6 4 259 51 301 1.33e-57 197
MS.gene64022.t1 AT4G11650 40.444 225 109 8 8 231 22 222 3.58e-46 156
MS.gene64022.t1 AT5G24620 56.667 120 47 3 116 231 15 133 1.19e-37 135
MS.gene64022.t1 AT2G24810 62.195 82 26 3 152 232 116 193 1.01e-26 104

Find 94 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCATGGGGCGGCCGTTTC+TGG -0.083846 1.3:-947508 MS.gene64022:CDS
CTCATGGGGCGGCCGTTTCT+GGG 0.253028 1.3:-947507 MS.gene64022:CDS
CCAGGGATTCTTTCAAATGC+TGG 0.316773 1.3:-947599 MS.gene64022:CDS
GCCAGAGCAATCAACCTTTC+CGG 0.319543 1.3:+947421 None:intergenic
AACAATTGAGAATAAGTAGA+AGG 0.345262 1.3:+947092 None:intergenic
CAGGGATTCTTTCAAATGCT+GGG 0.347178 1.3:-947598 MS.gene64022:CDS
CAGTGGTGGTTTCGCCGGTT+TGG 0.373152 1.3:+947543 None:intergenic
TATTGCTGCAGCGGCGCGTA+TGG 0.373787 1.3:-947134 MS.gene64022:CDS
TTCGATGTTAGTCTTGTTGA+CGG 0.393312 1.3:-947332 MS.gene64022:CDS
GGAAAATTCTCATGTGTAAC+CGG 0.404051 1.3:-947455 MS.gene64022:CDS
TGCACGCAAAAGTTTGTGAT+TGG 0.405228 1.3:+945975 None:intergenic
GTCGCCCCTCAGGGCGGCTC+TGG 0.409338 1.3:-947290 MS.gene64022:CDS
TCTCAACGGCGCGTGTCCTT+CGG 0.414761 1.3:-947234 MS.gene64022:CDS
TTAGCATCACTATGGTCCTT+TGG 0.416286 1.3:-945949 MS.gene64022:CDS
GCCTATGCTAGTCGCCCCTC+AGG 0.418663 1.3:-947300 MS.gene64022:CDS
TCCGGAAAGGTTGATTGCTC+TGG 0.435175 1.3:-947422 MS.gene64022:CDS
GTGAATTCGGCTAAAGTTGC+CGG 0.439691 1.3:+947383 None:intergenic
AGAGCCGTCTAATGTGAATT+CGG 0.464663 1.3:+947370 None:intergenic
TGTCACCGCACCAGCCTCAT+GGG 0.466682 1.3:-947522 MS.gene64022:CDS
GTGCATTATTAGCATCACTA+TGG 0.467599 1.3:-945957 MS.gene64022:CDS
ACAGGCTTTGTTCTCCAAAC+CGG 0.470298 1.3:-947557 MS.gene64022:CDS
GTAACCGGCGATTGTGGCTC+CGG 0.470556 1.3:-947440 MS.gene64022:CDS
GTAGAAGGTTTGCAAGTGTC+TGG 0.470850 1.3:+947107 None:intergenic
AAACGGCCGCCCCATGAGGC+TGG 0.484136 1.3:+947512 None:intergenic
CTGTCACCGCACCAGCCTCA+TGG 0.485359 1.3:-947523 MS.gene64022:CDS
CCAGCATTTGAAAGAATCCC+TGG 0.493036 1.3:+947599 None:intergenic
TGTGACTACACAGTGTGGCC+AGG 0.496467 1.3:-947617 MS.gene64022:CDS
GGTGACAGTGGTGGTTTCGC+CGG 0.497671 1.3:+947538 None:intergenic
AAAGAGTTCTGCCCCAGAAA+CGG 0.509995 1.3:+947495 None:intergenic
TCATGGGGCGGCCGTTTCTG+GGG 0.511165 1.3:-947506 MS.gene64022:CDS
GAAGATCTCATTAGAAGAGT+TGG 0.513485 1.3:+945918 None:intergenic
GCATCACTATGGTCCTTTGG+CGG 0.514552 1.3:-945946 MS.gene64022:CDS
AAAGTAACTAACCTTGTGGT+TGG 0.518082 1.3:+946966 None:intergenic
AAGCCTGTGGTTGGAAGTGT+TGG 0.519230 1.3:+947572 None:intergenic
TATCGCCAGAGCCGCCCTGA+GGG 0.521997 1.3:+947285 None:intergenic
CCCTGAGGGGCGACTAGCAT+AGG 0.525025 1.3:+947299 None:intergenic
TCATGTGTAACCGGCGATTG+TGG 0.527231 1.3:-947446 MS.gene64022:CDS
GAATTCACATTAGACGGCTC+TGG 0.528881 1.3:-947368 MS.gene64022:CDS
CTTTGCTCCCAAGACACTGA+TGG 0.532188 1.3:-947476 MS.gene64022:CDS
TTCACATTAGACGGCTCTGG+TGG 0.532251 1.3:-947365 MS.gene64022:CDS
ATTGCTGCAGCGGCGCGTAT+GGG 0.534091 1.3:-947133 MS.gene64022:CDS
GGCGATAATTGCACTACCAC+GGG 0.537099 1.3:-947269 MS.gene64022:CDS
GGCTAAAGTTGCCGGCGGTG+TGG 0.544415 1.3:+947391 None:intergenic
TTCAGTCAAAAGGCAACACA+AGG 0.545590 1.3:-946124 MS.gene64022:intron
ATAGCTATATGCACGTGGGC+AGG 0.548259 1.3:+947061 None:intergenic
CTTAGAGTGATGAGTGTGGA+TGG 0.554333 1.3:-947209 MS.gene64022:CDS
TGGAGAACAAAGCCTGTGGT+TGG 0.555388 1.3:+947563 None:intergenic
CCTATGCTAGTCGCCCCTCA+GGG 0.555587 1.3:-947299 MS.gene64022:CDS
AGGGATTCTTTCAAATGCTG+GGG 0.560190 1.3:-947597 MS.gene64022:CDS
GCCGCCCCATGAGGCTGGTG+CGG 0.562230 1.3:+947517 None:intergenic
GCGCCGCTGCAGCAATACTC+CGG 0.562265 1.3:+947140 None:intergenic
CTTTCCGGAGCCACAATCGC+CGG 0.563498 1.3:+947436 None:intergenic
TTTGTGAATATGTCATATGC+AGG 0.565168 1.3:-947674 None:intergenic
CGGCGATTGTGGCTCCGGAA+AGG 0.566510 1.3:-947435 MS.gene64022:CDS
AAGTTTGTGATTGGAGCACA+TGG 0.568630 1.3:+945984 None:intergenic
GTGACTACACAGTGTGGCCA+GGG 0.569936 1.3:-947616 MS.gene64022:CDS
GTCCCAACACTTCCAACCAC+AGG 0.572652 1.3:-947575 MS.gene64022:CDS
AAGGTTGATTGCTCTGGCAA+CGG 0.573575 1.3:-947416 MS.gene64022:CDS
TTAGCCGAATTCACATTAGA+CGG 0.576825 1.3:-947374 MS.gene64022:CDS
TGGCAACGGAGCCACACCGC+CGG 0.578628 1.3:-947402 MS.gene64022:CDS
ACCGCACCAGCCTCATGGGG+CGG 0.586044 1.3:-947518 MS.gene64022:CDS
ATGAGTGTGGATGGGAAAGA+CGG 0.592613 1.3:-947200 MS.gene64022:CDS
TGGCGATAATTGCACTACCA+CGG 0.593427 1.3:-947270 MS.gene64022:CDS
GACAATACAATGGTGTATGA+AGG 0.596541 1.3:-946046 MS.gene64022:CDS
CCAGAAACGGCCGCCCCATG+AGG 0.596649 1.3:+947508 None:intergenic
TCACCGGAGTATTGCTGCAG+CGG 0.602026 1.3:-947143 MS.gene64022:CDS
AGAGTTGGCACAACCGCCAA+AGG 0.605702 1.3:+945933 None:intergenic
ATTTGCACAAGTGAAAGTGC+TGG 0.609107 1.3:+947022 None:intergenic
TTGGAGCACATGGGTGCACA+TGG 0.612628 1.3:+945994 None:intergenic
TTATCGCCAGAGCCGCCCTG+AGG 0.617214 1.3:+947284 None:intergenic
AGCCTGTGGTTGGAAGTGTT+GGG 0.621679 1.3:+947573 None:intergenic
TCACATTAGACGGCTCTGGT+GGG 0.623574 1.3:-947364 MS.gene64022:CDS
TGAGGCTGGTGCGGTGACAG+TGG 0.623668 1.3:+947526 None:intergenic
ATCGCCAGAGCCGCCCTGAG+GGG 0.629555 1.3:+947286 None:intergenic
GTCATTGCTAAGCTGAGGTG+AGG 0.630131 1.3:+946066 None:intergenic
TTAGAGTGATGAGTGTGGAT+GGG 0.630518 1.3:-947208 MS.gene64022:CDS
GTCACCGCACCAGCCTCATG+GGG 0.632870 1.3:-947521 MS.gene64022:CDS
GGCTGGTGCGGTGACAGTGG+TGG 0.635446 1.3:+947529 None:intergenic
TTTGTTCTTCTCCAACCACA+AGG 0.638050 1.3:-946977 MS.gene64022:intron
CTCATCACTCTAAGCTCCGA+AGG 0.638536 1.3:+947218 None:intergenic
TAAGCATAGCTATATGCACG+TGG 0.665332 1.3:+947056 None:intergenic
GGATGTATCAGTGATCTCAA+CGG 0.673930 1.3:-947248 MS.gene64022:CDS
GCTTAGCAATGACAATACAA+TGG 0.678768 1.3:-946056 MS.gene64022:CDS
GAGATCACTGATACATCCCG+TGG 0.679790 1.3:+947253 None:intergenic
ATGCTAGTCGCCCCTCAGGG+CGG 0.689357 1.3:-947296 MS.gene64022:CDS
GTATTGTCATTGCTAAGCTG+AGG 0.695826 1.3:+946061 None:intergenic
GGTTTGGAGAACAAAGCCTG+TGG 0.698538 1.3:+947559 None:intergenic
AATTCGGCTAAAGTTGCCGG+CGG 0.706419 1.3:+947386 None:intergenic
AATACAATGGTGTATGAAGG+TGG 0.707551 1.3:-946043 MS.gene64022:CDS
TCAGTCAAAAGGCAACACAA+GGG 0.710852 1.3:-946123 MS.gene64022:intron
AAGCATAGCTATATGCACGT+GGG 0.721806 1.3:+947057 None:intergenic
GGAGCTTAGAGTGATGAGTG+TGG 0.722376 1.3:-947213 MS.gene64022:CDS
ACAAATGTGACTACACAGTG+TGG 0.729139 1.3:-947622 MS.gene64022:CDS
AGTTTGTGATTGGAGCACAT+GGG 0.786907 1.3:+945985 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGATTGATTATATTCAAT+AGG + chr1.3:946703-946722 None:intergenic 15.0%
!! AATAAATTGTTCAATGAACT+CGG - chr1.3:946867-946886 MS.gene64022:intron 20.0%
!! GACAAAATTCATTAATCATT+TGG + chr1.3:946843-946862 None:intergenic 20.0%
!! GTACATCAATCAATATAATT+TGG + chr1.3:947231-947250 None:intergenic 20.0%
!! TCATATTAGTTAAGATTTAC+CGG + chr1.3:946726-946745 None:intergenic 20.0%
!! TTGAATATAATCAATCTTAC+CGG - chr1.3:946704-946723 MS.gene64022:intron 20.0%
!!! AGAACAATTTTTCTATGAAT+CGG - chr1.3:946965-946984 MS.gene64022:intron 20.0%
!!! ATATAATTTGGTTCTAGATA+TGG + chr1.3:947219-947238 None:intergenic 20.0%
!!! TTATATTGATTGATGTACAT+TGG - chr1.3:947233-947252 MS.gene64022:CDS 20.0%
!!! TTTTTACGGTTATTATTTTG+AGG + chr1.3:946665-946684 None:intergenic 20.0%
! AAATAAAGTAACTAACCTTG+TGG + chr1.3:946634-946653 None:intergenic 25.0%
! AACAATTGAGAATAAGTAGA+AGG + chr1.3:946504-946523 None:intergenic 25.0%
! ATAAATTGTTCAATGAACTC+GGG - chr1.3:946868-946887 MS.gene64022:intron 25.0%
! TCAATAGAAATTGATGCAAA+GGG - chr1.3:947067-947086 MS.gene64022:CDS 25.0%
! TTCAATAGAAATTGATGCAA+AGG - chr1.3:947066-947085 MS.gene64022:CDS 25.0%
!! ATAAAGTTCATCACACTTTT+TGG + chr1.3:947342-947361 None:intergenic 25.0%
!! CTATTGTATTTTCAGTCAAA+AGG - chr1.3:947459-947478 MS.gene64022:CDS 25.0%
!! TCACAATGTAATTTTCCTAA+CGG + chr1.3:947367-947386 None:intergenic 25.0%
!!! GCACAATGGTAAATTTTTTA+CGG + chr1.3:946679-946698 None:intergenic 25.0%
ACATTGGATTCGCAAAATAT+GGG - chr1.3:947249-947268 MS.gene64022:CDS 30.0%
AGAAAAATTGTTCTGCTGTA+AGG + chr1.3:946959-946978 None:intergenic 30.0%
TACATTGGATTCGCAAAATA+TGG - chr1.3:947248-947267 MS.gene64022:CDS 30.0%
TATATTCAATAGGTGCACAA+TGG + chr1.3:946693-946712 None:intergenic 30.0%
TCCAAAATATCCAATGTGAT+AGG - chr1.3:947170-947189 MS.gene64022:CDS 30.0%
TTATCGTTACACAACACTAT+TGG - chr1.3:947290-947309 MS.gene64022:CDS 30.0%
! ACAATTTTTCTATGAATCGG+AGG - chr1.3:946968-946987 MS.gene64022:intron 30.0%
! ACCTATCACATTGGATATTT+TGG + chr1.3:947174-947193 None:intergenic 30.0%
! CAATTTTTCTATGAATCGGA+GGG - chr1.3:946969-946988 MS.gene64022:intron 30.0%
! CTGTAAGGTAATTTAGAGTT+CGG + chr1.3:946944-946963 None:intergenic 30.0%
! GAGTTTGATTCGATTAGATT+GGG - chr1.3:946776-946795 MS.gene64022:intron 30.0%
!! ACATTCATAACTATTTCTGC+CGG + chr1.3:947143-947162 None:intergenic 30.0%
!! CACATTGGATATTTTGGATT+TGG + chr1.3:947168-947187 None:intergenic 30.0%
AAAGTAACTAACCTTGTGGT+TGG + chr1.3:946630-946649 None:intergenic 35.0%
AAGTAGGTGAAGCAACATAA+TGG - chr1.3:947117-947136 MS.gene64022:CDS 35.0%
AATACAATGGTGTATGAAGG+TGG - chr1.3:947550-947569 MS.gene64022:CDS 35.0%
AATTGATTACCCACCAATCA+TGG + chr1.3:947047-947066 None:intergenic 35.0%
ATTGCTTGCAATTGCAAACA+TGG + chr1.3:947096-947115 None:intergenic 35.0%
GACAATACAATGGTGTATGA+AGG - chr1.3:947547-947566 MS.gene64022:CDS 35.0%
GCTTAGCAATGACAATACAA+TGG - chr1.3:947537-947556 MS.gene64022:CDS 35.0%
GGAAAATTCTCATGTGTAAC+CGG - chr1.3:946138-946157 MS.gene64022:intron 35.0%
GTGCATTATTAGCATCACTA+TGG - chr1.3:947636-947655 MS.gene64022:CDS 35.0%
TGATGAACTTTATGACCGTT+AGG - chr1.3:947349-947368 MS.gene64022:CDS 35.0%
TTAGCCGAATTCACATTAGA+CGG - chr1.3:946219-946238 MS.gene64022:intron 35.0%
! AGAATTTTCCATCAGTGTCT+TGG + chr1.3:946128-946147 None:intergenic 35.0%
! GAATTTTCCATCAGTGTCTT+GGG + chr1.3:946127-946146 None:intergenic 35.0%
! GGAGTTTGATTCGATTAGAT+TGG - chr1.3:946775-946794 MS.gene64022:intron 35.0%
!! ATGCGAAGCTTTTAATTCAC+CGG - chr1.3:946434-946453 MS.gene64022:intron 35.0%
!! TCCGAACTATTGGTTCATAA+GGG + chr1.3:946758-946777 None:intergenic 35.0%
!! TTCGATGTTAGTCTTGTTGA+CGG - chr1.3:946261-946280 MS.gene64022:intron 35.0%
AAGCATAGCTATATGCACGT+GGG + chr1.3:946539-946558 None:intergenic 40.0%
AAGTTTGTGATTGGAGCACA+TGG + chr1.3:947612-947631 None:intergenic 40.0%
ACAAATGTGACTACACAGTG+TGG - chr1.3:945971-945990 MS.gene64022:CDS 40.0%
AGAGCCGTCTAATGTGAATT+CGG + chr1.3:946226-946245 None:intergenic 40.0%
AGGGATTCTTTCAAATGCTG+GGG - chr1.3:945996-946015 MS.gene64022:CDS 40.0%
AGTTTGTGATTGGAGCACAT+GGG + chr1.3:947611-947630 None:intergenic 40.0%
ATGAACTCGGGTTCGATTAA+TGG - chr1.3:946880-946899 MS.gene64022:intron 40.0%
CAGGGATTCTTTCAAATGCT+GGG - chr1.3:945995-946014 MS.gene64022:CDS 40.0%
CCCCTTATGAACCAATAGTT+CGG - chr1.3:946754-946773 MS.gene64022:intron 40.0%
CGAATCAAACTCCGAACTAT+TGG + chr1.3:946768-946787 None:intergenic 40.0%
GCAATTGCAAGCAATCAAGT+AGG - chr1.3:947101-947120 MS.gene64022:CDS 40.0%
GGATGTATCAGTGATCTCAA+CGG - chr1.3:946345-946364 MS.gene64022:intron 40.0%
GGTCGCGAGGTAAATTATAA+CGG + chr1.3:946923-946942 None:intergenic 40.0%
GTATTGTCATTGCTAAGCTG+AGG + chr1.3:947535-947554 None:intergenic 40.0%
TAAGCATAGCTATATGCACG+TGG + chr1.3:946540-946559 None:intergenic 40.0%
TCAGTCAAAAGGCAACACAA+GGG - chr1.3:947470-947489 MS.gene64022:CDS 40.0%
TGAACTCGGGTTCGATTAAT+GGG - chr1.3:946881-946900 MS.gene64022:intron 40.0%
TGCACGCAAAAGTTTGTGAT+TGG + chr1.3:947621-947640 None:intergenic 40.0%
TGTTGTGTAACGATAACCCT+TGG + chr1.3:947286-947305 None:intergenic 40.0%
TTAGAGTGATGAGTGTGGAT+GGG - chr1.3:946385-946404 MS.gene64022:intron 40.0%
TTAGCATCACTATGGTCCTT+TGG - chr1.3:947644-947663 MS.gene64022:CDS 40.0%
TTCAGTCAAAAGGCAACACA+AGG - chr1.3:947469-947488 MS.gene64022:CDS 40.0%
TTGGATTCGCAAAATATGGG+TGG - chr1.3:947252-947271 MS.gene64022:CDS 40.0%
TTTGTTCTTCTCCAACCACA+AGG - chr1.3:946616-946635 MS.gene64022:intron 40.0%
! ATTTGCACAAGTGAAAGTGC+TGG + chr1.3:946574-946593 None:intergenic 40.0%
! TTTTAGCCACCATGATTGGT+GGG - chr1.3:947035-947054 MS.gene64022:CDS 40.0%
!! CCGAACTATTGGTTCATAAG+GGG + chr1.3:946757-946776 None:intergenic 40.0%
!! CTCCGAACTATTGGTTCATA+AGG + chr1.3:946759-946778 None:intergenic 40.0%
!! TGCTTTTTAGCCACCATGAT+TGG - chr1.3:947031-947050 MS.gene64022:CDS 40.0%
!! TTTTTAGCCACCATGATTGG+TGG - chr1.3:947034-947053 MS.gene64022:CDS 40.0%
AAAGAGTTCTGCCCCAGAAA+CGG + chr1.3:946101-946120 None:intergenic 45.0%
ACCAAGGAGACCTATCACAT+TGG + chr1.3:947183-947202 None:intergenic 45.0%
AGGTGAAGCAACATAATGGC+CGG - chr1.3:947121-947140 MS.gene64022:CDS 45.0%
ATGAGTGTGGATGGGAAAGA+CGG - chr1.3:946393-946412 MS.gene64022:intron 45.0%
CCAGCATTTGAAAGAATCCC+TGG + chr1.3:945997-946016 None:intergenic 45.0%
CCAGGGATTCTTTCAAATGC+TGG - chr1.3:945994-946013 MS.gene64022:CDS 45.0%
CTTAGAGTGATGAGTGTGGA+TGG - chr1.3:946384-946403 MS.gene64022:intron 45.0%
GAATTCACATTAGACGGCTC+TGG - chr1.3:946225-946244 MS.gene64022:intron 45.0%
GTAGAAGGTTTGCAAGTGTC+TGG + chr1.3:946489-946508 None:intergenic 45.0%
GTGAATTCGGCTAAAGTTGC+CGG + chr1.3:946213-946232 None:intergenic 45.0%
TCCAATGTGATAGGTCTCCT+TGG - chr1.3:947179-947198 MS.gene64022:CDS 45.0%
TGATTACCCACCAATCATGG+TGG + chr1.3:947044-947063 None:intergenic 45.0%
TGGCGATAATTGCACTACCA+CGG - chr1.3:946323-946342 MS.gene64022:intron 45.0%
! AAGGTTGATTGCTCTGGCAA+CGG - chr1.3:946177-946196 MS.gene64022:intron 45.0%
! TAATTTAGAGTTCGGTCGCG+AGG + chr1.3:946936-946955 None:intergenic 45.0%
!! ACAGGCTTTGTTCTCCAAAC+CGG - chr1.3:946036-946055 MS.gene64022:CDS 45.0%
AATTCGGCTAAAGTTGCCGG+CGG + chr1.3:946210-946229 None:intergenic 50.0%
ATAGCTATATGCACGTGGGC+AGG + chr1.3:946535-946554 None:intergenic 50.0%
CTCATCACTCTAAGCTCCGA+AGG + chr1.3:946378-946397 None:intergenic 50.0%
CTTTGCTCCCAAGACACTGA+TGG - chr1.3:946117-946136 MS.gene64022:CDS 50.0%
GAGATCACTGATACATCCCG+TGG + chr1.3:946343-946362 None:intergenic 50.0%
GCATCACTATGGTCCTTTGG+CGG - chr1.3:947647-947666 MS.gene64022:CDS 50.0%
GCCAGAGCAATCAACCTTTC+CGG + chr1.3:946175-946194 None:intergenic 50.0%
GGAGCTTAGAGTGATGAGTG+TGG - chr1.3:946380-946399 MS.gene64022:intron 50.0%
GGCGATAATTGCACTACCAC+GGG - chr1.3:946324-946343 MS.gene64022:intron 50.0%
GGTTTGGAGAACAAAGCCTG+TGG + chr1.3:946037-946056 None:intergenic 50.0%
GTCATTGCTAAGCTGAGGTG+AGG + chr1.3:947530-947549 None:intergenic 50.0%
GTCTCCTTGGTCAGGTACTA+GGG - chr1.3:947192-947211 MS.gene64022:CDS 50.0%
TCATGTGTAACCGGCGATTG+TGG - chr1.3:946147-946166 MS.gene64022:intron 50.0%
TGGAGAACAAAGCCTGTGGT+TGG + chr1.3:946033-946052 None:intergenic 50.0%
TGTGATAGGTCTCCTTGGTC+AGG - chr1.3:947184-947203 MS.gene64022:CDS 50.0%
! AAGCCTGTGGTTGGAAGTGT+TGG + chr1.3:946024-946043 None:intergenic 50.0%
! TCACATTAGACGGCTCTGGT+GGG - chr1.3:946229-946248 MS.gene64022:intron 50.0%
! TCCGGAAAGGTTGATTGCTC+TGG - chr1.3:946171-946190 MS.gene64022:intron 50.0%
! TTCACATTAGACGGCTCTGG+TGG - chr1.3:946228-946247 MS.gene64022:intron 50.0%
!! AGCCTGTGGTTGGAAGTGTT+GGG + chr1.3:946023-946042 None:intergenic 50.0%
AGAGTTGGCACAACCGCCAA+AGG + chr1.3:947663-947682 None:intergenic 55.0%
ATGGGTGGCACACATGCCAA+GGG - chr1.3:947267-947286 MS.gene64022:CDS 55.0%
GGTCTCCTTGGTCAGGTACT+AGG - chr1.3:947191-947210 MS.gene64022:CDS 55.0%
GTCCCAACACTTCCAACCAC+AGG - chr1.3:946018-946037 MS.gene64022:CDS 55.0%
GTGACTACACAGTGTGGCCA+GGG - chr1.3:945977-945996 MS.gene64022:CDS 55.0%
TATGGGTGGCACACATGCCA+AGG - chr1.3:947266-947285 MS.gene64022:CDS 55.0%
TGGTCCCTAGTACCTGACCA+AGG + chr1.3:947199-947218 None:intergenic 55.0%
TGTGACTACACAGTGTGGCC+AGG - chr1.3:945976-945995 MS.gene64022:CDS 55.0%
TTGGAGCACATGGGTGCACA+TGG + chr1.3:947602-947621 None:intergenic 55.0%
!! TCACCGGAGTATTGCTGCAG+CGG - chr1.3:946450-946469 MS.gene64022:intron 55.0%
ATTGCTGCAGCGGCGCGTAT+GGG - chr1.3:946460-946479 MS.gene64022:intron 60.0%
CCTATGCTAGTCGCCCCTCA+GGG - chr1.3:946294-946313 MS.gene64022:intron 60.0%
CTTTCCGGAGCCACAATCGC+CGG + chr1.3:946160-946179 None:intergenic 60.0%
GGTGACAGTGGTGGTTTCGC+CGG + chr1.3:946058-946077 None:intergenic 60.0%
GTAACCGGCGATTGTGGCTC+CGG - chr1.3:946153-946172 MS.gene64022:intron 60.0%
TATTGCTGCAGCGGCGCGTA+TGG - chr1.3:946459-946478 MS.gene64022:intron 60.0%
TCTCAACGGCGCGTGTCCTT+CGG - chr1.3:946359-946378 MS.gene64022:intron 60.0%
TGTCACCGCACCAGCCTCAT+GGG - chr1.3:946071-946090 MS.gene64022:CDS 60.0%
! CAGTGGTGGTTTCGCCGGTT+TGG + chr1.3:946053-946072 None:intergenic 60.0%
ATGCTAGTCGCCCCTCAGGG+CGG - chr1.3:946297-946316 MS.gene64022:intron 65.0%
CCCTGAGGGGCGACTAGCAT+AGG + chr1.3:946297-946316 None:intergenic 65.0%
CGGCGATTGTGGCTCCGGAA+AGG - chr1.3:946158-946177 MS.gene64022:intron 65.0%
CTCATGGGGCGGCCGTTTCT+GGG - chr1.3:946086-946105 MS.gene64022:CDS 65.0%
CTGTCACCGCACCAGCCTCA+TGG - chr1.3:946070-946089 MS.gene64022:CDS 65.0%
GCCTATGCTAGTCGCCCCTC+AGG - chr1.3:946293-946312 MS.gene64022:intron 65.0%
GCGCCGCTGCAGCAATACTC+CGG + chr1.3:946456-946475 None:intergenic 65.0%
GGCTAAAGTTGCCGGCGGTG+TGG + chr1.3:946205-946224 None:intergenic 65.0%
GTCACCGCACCAGCCTCATG+GGG - chr1.3:946072-946091 MS.gene64022:CDS 65.0%
TATCGCCAGAGCCGCCCTGA+GGG + chr1.3:946311-946330 None:intergenic 65.0%
TCATGGGGCGGCCGTTTCTG+GGG - chr1.3:946087-946106 MS.gene64022:CDS 65.0%
TGAGGCTGGTGCGGTGACAG+TGG + chr1.3:946070-946089 None:intergenic 65.0%
TTATCGCCAGAGCCGCCCTG+AGG + chr1.3:946312-946331 None:intergenic 65.0%
AAACGGCCGCCCCATGAGGC+TGG + chr1.3:946084-946103 None:intergenic 70.0%
ACCGCACCAGCCTCATGGGG+CGG - chr1.3:946075-946094 MS.gene64022:CDS 70.0%
ATCGCCAGAGCCGCCCTGAG+GGG + chr1.3:946310-946329 None:intergenic 70.0%
CCAGAAACGGCCGCCCCATG+AGG + chr1.3:946088-946107 None:intergenic 70.0%
CCTCATGGGGCGGCCGTTTC+TGG - chr1.3:946085-946104 MS.gene64022:CDS 70.0%
GGCTGGTGCGGTGACAGTGG+TGG + chr1.3:946067-946086 None:intergenic 70.0%
TGGCAACGGAGCCACACCGC+CGG - chr1.3:946191-946210 MS.gene64022:intron 70.0%
GCCGCCCCATGAGGCTGGTG+CGG + chr1.3:946079-946098 None:intergenic 75.0%
! GTCGCCCCTCAGGGCGGCTC+TGG - chr1.3:946303-946322 MS.gene64022:intron 80.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 945928 947687 945928 ID=MS.gene64022
chr1.3 mRNA 945928 947687 945928 ID=MS.gene64022.t1;Parent=MS.gene64022
chr1.3 exon 946978 947687 946978 ID=MS.gene64022.t1.exon1;Parent=MS.gene64022.t1
chr1.3 CDS 946978 947687 946978 ID=cds.MS.gene64022.t1;Parent=MS.gene64022.t1
chr1.3 exon 945928 946141 945928 ID=MS.gene64022.t1.exon2;Parent=MS.gene64022.t1
chr1.3 CDS 945928 946141 945928 ID=cds.MS.gene64022.t1;Parent=MS.gene64022.t1
Gene Sequence

>MS.gene64022

ATGTCATATGCAGGTGTAATTTCAACAACATTCACATTTGTTAACAAATGTGACTACACAGTGTGGCCAGGGATTCTTTCAAATGCTGGGGTCCCAACACTTCCAACCACAGGCTTTGTTCTCCAAACCGGCGAAACCACCACTGTCACCGCACCAGCCTCATGGGGCGGCCGTTTCTGGGGCAGAACTCTTTGCTCCCAAGACACTGATGGAAAATTCTCATGTGTAACCGGCGATTGTGGCTCCGGAAAGGTTGATTGCTCTGGCAACGGAGCCACACCGCCGGCAACTTTAGCCGAATTCACATTAGACGGCTCTGGTGGGCTTGACTTCTTCGATGTTAGTCTTGTTGACGGTTACAATGTGCCTATGCTAGTCGCCCCTCAGGGCGGCTCTGGCGATAATTGCACTACCACGGGATGTATCAGTGATCTCAACGGCGCGTGTCCTTCGGAGCTTAGAGTGATGAGTGTGGATGGGAAAGACGGTGTCGCGTGTAAGAGCGCATGCGAAGCTTTTAATTCACCGGAGTATTGCTGCAGCGGCGCGTATGGGACACCAGACACTTGCAAACCTTCTACTTATTCTCAATTGTTTAAGAACGCCTGCCCACGTGCATATAGCTATGCTTATGATGATAAGACCAGCACTTTCACTTGTGCAAATGCAGTAGATTATACTATCACATTTTGTTCTTCTCCAACCACAAGGTTAGTTACTTTATTTTTACTTCTCCTCAAAATAATAACCGTAAAAAATTTACCATTGTGCACCTATTGAATATAATCAATCTTACCGGTAAATCTTAACTAATATGATGATTCAACCCCTTATGAACCAATAGTTCGGAGTTTGATTCGATTAGATTGGGTAATGTATACAAGTGTTCTTTGTTTAGTAAAGTAGATCAAACCAAATGATTAATGAATTTTGTCAAATAATAAATTGTTCAATGAACTCGGGTTCGATTAATGGGTGAAACAATTTTTTATCCGTTATAATTTACCTCGCGACCGAACTCTAAATTACCTTACAGCAGAACAATTTTTCTATGAATCGGAGGGTTAATAGAACAAAAAAAGAATAACGATTTAATAGATGTTTGCTTTTTAGCCACCATGATTGGTGGGTAATCAATTTCAATAGAAATTGATGCAAAGGGAAACCATGTTTGCAATTGCAAGCAATCAAGTAGGTGAAGCAACATAATGGCCGGCAGAAATAGTTATGAATGTCACCAAATCCAAAATATCCAATGTGATAGGTCTCCTTGGTCAGGTACTAGGGACCATATCTAGAACCAAATTATATTGATTGATGTACATTGGATTCGCAAAATATGGGTGGCACACATGCCAAGGGTTATCGTTACACAACACTATTGGTAATATGTCTACATCAAAATAAGTTACCAAAAAGTGTGATGAACTTTATGACCGTTAGGAAAATTACATTGTGACTCTTATTTTACATGTGATATTGTTATGTCTATTGTTGCATTTTTATTTTTTTTAGATTTTTGATCATTTTTCTATTGTATTTTCAGTCAAAAGGCAACACAAGGGCAAGACACAAAACAAGAGAGTTCTTCTAACTCTGCCTCACCTCAGCTTAGCAATGACAATACAATGGTGTATGAAGGTGGTATTGATCAAAGTGAAATTTCACAAGCCATGTGCACCCATGTGCTCCAATCACAAACTTTTGCGTGCATTATTAGCATCACTATGGTCCTTTGGCGGTTGTGCCAACTCTTCTAA
Protein sequence

>MS.gene64022.t1

MSYAGVISTTFTFVNKCDYTVWPGILSNAGVPTLPTTGFVLQTGETTTVTAPASWGGRFWGRTLCSQDTDGKFSCVTGDCGSGKVDCSGNGATPPATLAEFTLDGSGGLDFFDVSLVDGYNVPMLVAPQGGSGDNCTTTGCISDLNGACPSELRVMSVDGKDGVACKSACEAFNSPEYCCSGAYGTPDTCKPSTYSQLFKNACPRAYSYAYDDKTSTFTCANAVDYTITFCSSPTTSQKATQGQDTKQESSSNSASPQLSNDNTMVYEGGIDQSEISQAMCTHVLQSQTFACIISITMVLWRLCQLF