AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42251

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42251.t1 MTR_1g025420 74.164 329 73 7 1 323 1 323 4.74e-171 478
MS.gene42251.t1 MTR_3g114030 97.881 236 5 0 20 255 1 236 4.02e-169 469
MS.gene42251.t1 MTR_1g025420 81.600 250 43 2 1 250 1 247 8.96e-149 419
MS.gene42251.t1 MTR_5g022310 68.582 261 77 4 8 265 8 266 1.21e-119 347
MS.gene42251.t1 MTR_5g022310 70.902 244 67 3 8 248 8 250 1.32e-118 342
MS.gene42251.t1 MTR_8g037890 64.469 273 90 5 5 275 7 274 6.94e-116 337
MS.gene42251.t1 MTR_8g075550 68.254 252 72 4 19 265 21 269 3.85e-114 333
MS.gene42251.t1 MTR_8g056820 67.083 240 75 3 10 248 10 246 1.83e-111 324
MS.gene42251.t1 MTR_4g073730 63.786 243 85 3 8 248 6 247 1.35e-106 313
MS.gene42251.t1 MTR_4g073730 63.786 243 85 3 8 248 6 247 3.80e-106 313
MS.gene42251.t1 MTR_4g073720 54.296 291 124 6 1 291 1 282 2.37e-96 289
MS.gene42251.t1 MTR_8g075510 57.200 250 97 4 3 252 8 247 1.30e-92 276
MS.gene42251.t1 MTR_8g075510 58.130 246 93 5 3 248 8 243 4.60e-92 276
MS.gene42251.t1 MTR_5g022350 53.261 276 110 7 11 281 19 280 5.84e-89 269
MS.gene42251.t1 MTR_7g102380 55.462 238 103 3 14 251 9 243 6.99e-89 266
MS.gene42251.t1 MTR_2g068655 54.918 244 106 3 6 248 11 251 1.38e-84 258
MS.gene42251.t1 MTR_2g067980 53.696 257 110 6 11 262 8 260 5.73e-84 273
MS.gene42251.t1 MTR_2g068030 53.165 237 105 5 20 254 17 249 6.57e-84 254
MS.gene42251.t1 MTR_8g036215 52.245 245 109 6 4 248 1 237 4.29e-80 243
MS.gene42251.t1 MTR_1g062590 54.955 222 97 3 27 248 23 241 1.67e-79 242
MS.gene42251.t1 MTR_2g069660 50.628 239 115 3 11 248 6 242 8.35e-79 240
MS.gene42251.t1 MTR_6g079580 50.826 242 114 4 7 248 3 239 2.98e-78 239
MS.gene42251.t1 MTR_1g021945 56.195 226 91 6 25 248 25 244 4.07e-77 236
MS.gene42251.t1 MTR_1g062340 52.720 239 108 4 12 250 10 243 5.47e-77 236
MS.gene42251.t1 MTR_1g062660 53.361 238 105 4 13 250 12 243 8.53e-77 235
MS.gene42251.t1 MTR_1g062350 52.439 246 112 4 5 250 3 243 1.05e-76 235
MS.gene42251.t1 MTR_1g062370 52.033 246 113 4 5 250 3 243 1.36e-76 235
MS.gene42251.t1 MTR_1g062630 53.571 224 101 3 29 252 25 245 1.99e-76 235
MS.gene42251.t1 MTR_3g111620 53.975 239 103 7 12 248 12 245 9.55e-74 228
MS.gene42251.t1 MTR_8g107140 49.796 245 118 4 4 248 5 244 1.81e-72 224
MS.gene42251.t1 MTR_3g068015 46.964 247 120 8 8 248 6 247 7.68e-65 205
MS.gene42251.t1 MTR_6g009480 45.669 254 129 7 1 248 1 251 9.34e-65 206
MS.gene42251.t1 MTR_5g059200 46.154 247 121 8 3 249 6 240 6.47e-63 201
MS.gene42251.t1 MTR_7g062610 46.185 249 125 7 1 248 1 241 2.35e-61 196
MS.gene42251.t1 MTR_1g062690 46.748 246 102 5 5 250 3 219 6.05e-61 194
MS.gene42251.t1 MTR_4g063630 43.210 243 126 8 11 249 18 252 5.00e-59 190
MS.gene42251.t1 MTR_2g067990 46.091 243 101 8 21 262 22 235 3.79e-55 193
MS.gene42251.t1 MTR_5g023850 49.735 189 88 4 3 186 1 187 7.17e-54 176
MS.gene42251.t1 MTR_5g010640 41.532 248 116 8 9 248 9 235 1.02e-50 168
MS.gene42251.t1 MTR_2g067970 45.455 198 99 7 69 262 3 195 5.71e-47 169
MS.gene42251.t1 MTR_5g010635 39.556 225 112 7 25 248 23 224 1.26e-45 155
MS.gene42251.t1 MTR_8g096900 39.024 246 122 9 6 248 27 247 7.04e-45 154
MS.gene42251.t1 MTR_5g022350 56.429 140 54 3 11 150 84 216 2.44e-44 151
MS.gene42251.t1 MTR_2g063150 31.873 251 155 6 1 242 1 244 1.84e-38 137
MS.gene42251.t1 MTR_8g089020 51.538 130 57 3 111 240 33 156 8.16e-37 130
MS.gene42251.t1 MTR_7g076360 36.697 218 73 4 25 242 7 159 7.73e-36 127
MS.gene42251.t1 MTR_3g114040 91.549 71 5 1 256 325 33 103 2.56e-35 124
MS.gene42251.t1 MTR_3g081550 62.766 94 35 0 111 204 16 109 2.63e-34 122
MS.gene42251.t1 MTR_1g062390 30.116 259 149 9 2 250 3 239 2.00e-29 113
MS.gene42251.t1 MTR_8g096910 42.254 142 68 5 1 141 1 129 1.98e-26 103
MS.gene42251.t1 MTR_8g096920 40.909 132 68 4 11 141 4 126 4.15e-21 89.0
MS.gene42251.t1 MTR_1g062640 42.708 96 53 2 72 167 53 146 3.90e-15 72.0
MS.gene42251.t1 MTR_5g068670 48.529 68 33 2 77 144 25 90 3.16e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42251.t1 AT1G75800 61.194 335 111 5 4 325 1 329 8.34e-137 391
MS.gene42251.t1 AT1G20030 64.379 306 101 4 22 325 16 315 2.70e-133 382
MS.gene42251.t1 AT1G20030 64.145 304 101 4 24 325 1 298 9.21e-132 377
MS.gene42251.t1 AT4G36010 63.740 262 84 6 22 276 19 276 1.53e-110 323
MS.gene42251.t1 AT4G36010 63.740 262 84 6 22 276 19 276 1.53e-110 323
MS.gene42251.t1 AT2G17860 63.265 245 85 4 10 250 7 250 7.18e-105 307
MS.gene42251.t1 AT4G38670 54.386 285 119 4 4 287 1 275 1.03e-104 308
MS.gene42251.t1 AT4G38670 54.386 285 119 4 4 287 1 275 1.03e-104 308
MS.gene42251.t1 AT4G38670 63.043 230 84 1 20 248 1 230 4.04e-103 303
MS.gene42251.t1 AT4G38660 55.797 276 105 6 3 272 7 271 2.36e-95 286
MS.gene42251.t1 AT4G24180 57.778 270 99 5 12 275 19 279 5.33e-94 282
MS.gene42251.t1 AT4G24180 56.475 278 106 5 4 275 6 274 8.24e-94 281
MS.gene42251.t1 AT4G38660 58.687 259 90 6 20 272 2 249 2.62e-93 280
MS.gene42251.t1 AT4G24180 60.000 245 86 4 12 250 19 257 7.21e-93 277
MS.gene42251.t1 AT4G24180 58.498 253 93 4 4 250 6 252 1.02e-92 276
MS.gene42251.t1 AT5G38280 53.755 253 115 2 11 261 10 262 1.03e-92 290
MS.gene42251.t1 AT5G38280 53.755 253 115 2 11 261 10 262 2.39e-92 289
MS.gene42251.t1 AT4G24180 51.838 272 112 5 12 277 19 277 7.08e-85 259
MS.gene42251.t1 AT4G24180 50.714 280 119 5 4 277 6 272 7.21e-85 259
MS.gene42251.t1 AT5G24620 50.735 272 117 6 13 279 12 271 1.30e-82 256
MS.gene42251.t1 AT5G24620 50.735 272 117 6 13 279 67 326 3.38e-82 257
MS.gene42251.t1 AT5G24620 50.735 272 117 6 13 279 88 347 4.50e-82 257
MS.gene42251.t1 AT1G19320 54.618 249 102 7 5 249 6 247 4.84e-81 246
MS.gene42251.t1 AT1G75040 56.017 241 96 7 10 248 6 238 6.87e-80 243
MS.gene42251.t1 AT1G75050 51.822 247 114 5 6 252 5 246 9.73e-80 243
MS.gene42251.t1 AT1G75030 51.429 245 111 7 7 248 3 242 4.32e-77 236
MS.gene42251.t1 AT1G18250 51.528 229 106 4 25 253 20 243 5.30e-75 231
MS.gene42251.t1 AT1G18250 51.528 229 106 4 25 253 21 244 6.17e-75 231
MS.gene42251.t1 AT1G73620 52.232 224 102 4 25 248 41 259 1.08e-73 228
MS.gene42251.t1 AT1G77700 48.606 251 109 9 4 250 39 273 3.79e-69 219
MS.gene42251.t1 AT2G28790 47.036 253 126 7 1 249 26 274 6.19e-69 216
MS.gene42251.t1 AT1G77700 48.000 250 118 8 4 253 39 276 7.06e-69 218
MS.gene42251.t1 AT2G28790 47.036 253 126 7 1 249 1 249 9.68e-69 215
MS.gene42251.t1 AT1G77700 50.435 230 94 9 25 250 88 301 1.37e-66 213
MS.gene42251.t1 AT5G40020 45.059 253 132 6 1 250 1 249 1.77e-62 199
MS.gene42251.t1 AT4G18253 42.742 248 132 4 4 250 1 239 4.02e-62 198
MS.gene42251.t1 AT4G36000 52.444 225 82 8 102 323 1 203 1.15e-61 196
MS.gene42251.t1 AT4G36000 52.444 225 82 8 102 323 1 203 1.15e-61 196
MS.gene42251.t1 AT1G70250 44.351 239 120 6 24 256 143 374 1.86e-60 206
MS.gene42251.t1 AT4G18250 42.294 279 151 6 11 287 41 311 7.48e-60 203
MS.gene42251.t1 AT5G02140 44.672 244 129 6 6 248 2 240 1.08e-59 192
MS.gene42251.t1 AT5G02140 44.672 244 129 6 6 248 2 240 1.66e-59 193
MS.gene42251.t1 AT4G11650 40.244 246 122 8 4 248 1 222 2.78e-48 162
MS.gene42251.t1 AT5G24620 50.318 157 63 4 133 285 15 160 3.94e-40 143
MS.gene42251.t1 AT2G24810 58.025 81 31 2 169 249 116 193 5.49e-23 94.7

Find 101 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTGGGGAGGCCGTTTC+TGG -0.052359 3.2:+90399373 MS.gene42251:CDS
ATACTTAATCTTTCAGTATC+AGG 0.193263 3.2:+90399207 MS.gene42251:CDS
CAATCACCCCACCCGCTTCC+TGG 0.293774 3.2:+90399358 MS.gene42251:CDS
GTACGAATACCGGTTGTATC+AGG 0.300485 3.2:+90399622 MS.gene42251:CDS
TTCCTGGGGAGGCCGTTTCT+GGG 0.311657 3.2:+90399374 MS.gene42251:CDS
GGAAAATTCTCCTGTCTTAC+CGG 0.321634 3.2:+90399426 MS.gene42251:CDS
AGCGGGTGGGGTGATTGATT+TGG 0.328396 3.2:-90399352 None:intergenic
TGGTGGTGTTGCGCCGTTGC+CGG 0.342266 3.2:-90399478 None:intergenic
GGGTCACTGCCATCTGATTT+CGG 0.345175 3.2:-90401112 None:intergenic
CCGGCGTTGGAGAGAATTCC+CGG 0.381681 3.2:-90399282 None:intergenic
TCACCTTGGGCTGTTGTTAG+TGG 0.384731 3.2:-90399913 None:intergenic
TTGTGATGGTAAGAATAGTT+TGG 0.385951 3.2:-90401237 None:intergenic
GCCGGAGCATTCGACTTTGC+CGG 0.389067 3.2:-90399460 None:intergenic
TGTGATGGTAAGAATAGTTT+GGG 0.391149 3.2:-90401236 None:intergenic
GCCTCCCCAGGAAGCGGGTG+GGG 0.392476 3.2:-90399364 None:intergenic
AATCACCCCACCCGCTTCCT+GGG 0.417844 3.2:+90399359 MS.gene42251:CDS
CGCGTGTCCTTCAGAGCTTA+AGG 0.433220 3.2:+90399656 MS.gene42251:CDS
TTGTTAAGCTGAGGCGATGA+TGG 0.438481 3.2:-90401133 None:intergenic
ACAAATGCAACTACACAGTA+TGG 0.441553 3.2:+90399259 MS.gene42251:CDS
ACCGGTTTCGTTCTTCAATC+CGG 0.449618 3.2:+90399324 MS.gene42251:CDS
TTAGCAGAATTCACTCTTGA+CGG 0.450720 3.2:+90399507 MS.gene42251:CDS
TATTGTTGCAGCGGCGCGTA+TGG 0.455532 3.2:+90399747 MS.gene42251:CDS
CTGAAAGATTAAGTATGATA+AGG 0.455964 3.2:-90399200 None:intergenic
TTGCAGTCAAAAGTCGTGGC+AGG 0.458754 3.2:+90401081 MS.gene42251:intron
CTTAAGGTTACGAGCGTGGA+TGG 0.458977 3.2:+90399672 MS.gene42251:CDS
TACTTAATCTTTCAGTATCA+GGG 0.459190 3.2:+90399208 MS.gene42251:CDS
GCCCCAGAAACGGCCTCCCC+AGG 0.462616 3.2:-90399376 None:intergenic
TTGATCAGAGTGAAATATCA+TGG 0.466410 3.2:+90401188 MS.gene42251:CDS
CTCGAAGGTTTACAAGTGTT+AGG 0.467705 3.2:-90399774 None:intergenic
AAAAGTGCTTGTGAGGCGTT+TGG 0.468766 3.2:+90399714 MS.gene42251:CDS
GAATTCACTCTTGACGGATC+CGG 0.482346 3.2:+90399513 MS.gene42251:CDS
AGAGAGTGCGGCCCCAGAAA+CGG 0.490637 3.2:-90399386 None:intergenic
AAGCGGTGCAACGCCGGCGT+TGG 0.494696 3.2:-90399295 None:intergenic
GGTTGGAAGCGGTGCAACGC+CGG 0.499056 3.2:-90399301 None:intergenic
ATGCAACTACACAGTATGGC+CGG 0.501235 3.2:+90399263 MS.gene42251:CDS
ACCCCACCCGCTTCCTGGGG+AGG 0.502582 3.2:+90399363 MS.gene42251:CDS
TAACAACGGCTCTGGCTCAA+TGG 0.504154 3.2:+90401150 MS.gene42251:CDS
GTGAATTCTGCTAAGGTAGC+TGG 0.506706 3.2:-90399498 None:intergenic
GTAGTTCCACAAGGTGGCTC+CGG 0.507977 3.2:+90399591 MS.gene42251:CDS
GGCTCAATGGTGTACGTTGG+TGG 0.508308 3.2:+90401163 MS.gene42251:CDS
GTTGCACCGCTTCCAACCAC+CGG 0.509906 3.2:+90399306 MS.gene42251:CDS
CGGCCTCCCCAGGAAGCGGG+TGG 0.513133 3.2:-90399366 None:intergenic
GCCGGATTGAAGAACGAAAC+CGG 0.514473 3.2:-90399325 None:intergenic
GAAACGGCCTCCCCAGGAAG+CGG 0.516631 3.2:-90399370 None:intergenic
AAACGGCCTCCCCAGGAAGC+GGG 0.522887 3.2:-90399369 None:intergenic
GGTGATAACTGTACGAATAC+CGG 0.523090 3.2:+90399612 MS.gene42251:CDS
TTCGACGTGAGTTTAGTTGA+CGG 0.526291 3.2:+90399549 MS.gene42251:CDS
CCTTGTGGAACTACAAGCAT+TGG 0.527175 3.2:-90399582 None:intergenic
CCTCAGCTTAACAACGGCTC+TGG 0.530822 3.2:+90401142 MS.gene42251:CDS
GTTACAATCACCGGTAAGAC+AGG 0.531262 3.2:-90399436 None:intergenic
TCCTGGGGAGGCCGTTTCTG+GGG 0.533325 3.2:+90399375 MS.gene42251:CDS
CCTTGGGCTGTTGTTAGTGG+AGG 0.540523 3.2:-90399910 None:intergenic
GAGCAAATCCCTGATACAAC+CGG 0.543946 3.2:-90399631 None:intergenic
GGTGATTGATTTGGAGTCGC+CGG 0.547268 3.2:-90399343 None:intergenic
AAAGTCGAATGCTCCGGCAA+CGG 0.549699 3.2:+90399465 MS.gene42251:CDS
TGCTTGTGAGGCGTTTGGAA+CGG 0.555408 3.2:+90399719 MS.gene42251:CDS
TCCGGCAAAGTCGAATGCTC+CGG 0.561093 3.2:+90399459 MS.gene42251:CDS
CTCGTAACCTTAAGCTCTGA+AGG 0.561863 3.2:-90399663 None:intergenic
CACGTTTACATGTGCTTCTG+CGG 0.562492 3.2:+90399863 MS.gene42251:CDS
ACGAAACCGGTGGTTGGAAG+CGG 0.564357 3.2:-90399312 None:intergenic
GGCCTCCCCAGGAAGCGGGT+GGG 0.565175 3.2:-90399365 None:intergenic
CTTACCGGTGATTGTAACTC+CGG 0.565383 3.2:+90399441 MS.gene42251:CDS
TGCAACTACACAGTATGGCC+GGG 0.567121 3.2:+90399264 MS.gene42251:CDS
TGTTAAGCTGAGGCGATGAT+GGG 0.569118 3.2:-90401132 None:intergenic
ATCACCCCACCCGCTTCCTG+GGG 0.571380 3.2:+90399360 MS.gene42251:CDS
CCGGGAATTCTCTCCAACGC+CGG 0.573274 3.2:+90399282 MS.gene42251:CDS
AGAGCTTAAGGTTACGAGCG+TGG 0.575561 3.2:+90399668 MS.gene42251:CDS
TTCACTCTTGACGGATCCGG+TGG 0.575766 3.2:+90399516 MS.gene42251:CDS
TACGAATACCGGTTGTATCA+GGG 0.577024 3.2:+90399623 MS.gene42251:CDS
TGCAGTCAAAAGTCGTGGCA+GGG 0.577535 3.2:+90401082 MS.gene42251:intron
CTCTGTTCACAAGACTCCAC+CGG 0.581870 3.2:+90399405 MS.gene42251:CDS
TGAAGAACGAAACCGGTGGT+TGG 0.582201 3.2:-90399318 None:intergenic
TTACCATCACAACGGCTATG+TGG 0.583574 3.2:+90401248 MS.gene42251:CDS
GTCAAGAGTGAATTCTGCTA+AGG 0.593003 3.2:-90399505 None:intergenic
GGACAAAATCCGAAATCAGA+TGG 0.593450 3.2:+90401103 MS.gene42251:CDS
AAGCCACATAGCCGTTGTGA+TGG 0.604066 3.2:-90401251 None:intergenic
TGATCAGAGTGAAATATCAT+GGG 0.606552 3.2:+90401189 MS.gene42251:CDS
TTAAGGTTACGAGCGTGGAT+GGG 0.608209 3.2:+90399673 MS.gene42251:CDS
AACAACTCAGAATAACTCGA+AGG 0.608498 3.2:-90399789 None:intergenic
TTTGCCGGAGTTACAATCAC+CGG 0.611025 3.2:-90399445 None:intergenic
GGTTGTATCAGGGATTTGCT+CGG 0.611595 3.2:+90399633 MS.gene42251:CDS
CCAATGCTTGTAGTTCCACA+AGG 0.616622 3.2:+90399582 MS.gene42251:CDS
TTATCACCGGAGCCACCTTG+TGG 0.619692 3.2:-90399597 None:intergenic
ACTTAATCTTTCAGTATCAG+GGG 0.627436 3.2:+90399209 MS.gene42251:CDS
ACGGCACAATATTGTTGCAG+CGG 0.630605 3.2:+90399738 MS.gene42251:CDS
GTCGAAGAAATCGAGTCCAC+CGG 0.632997 3.2:-90399532 None:intergenic
CCAGAGCCGTTGTTAAGCTG+AGG 0.634119 3.2:-90401142 None:intergenic
TCTGGCTCAATGGTGTACGT+TGG 0.639261 3.2:+90401160 MS.gene42251:CDS
GGCGTGTAAAAGTGCTTGTG+AGG 0.640993 3.2:+90399707 MS.gene42251:CDS
CGTGGATGGGAAAGAGAGCG+TGG 0.649504 3.2:+90399686 MS.gene42251:CDS
CCTCCACTAACAACAGCCCA+AGG 0.650691 3.2:+90399910 MS.gene42251:CDS
GGTATTCGTACAGTTATCAC+CGG 0.657314 3.2:-90399610 None:intergenic
TTTCCACACATTGATACACG+TGG 0.670795 3.2:-90401212 None:intergenic
AACTATTCTTACCATCACAA+CGG 0.673194 3.2:+90401240 MS.gene42251:CDS
GGGCCACGTGTATCAATGTG+TGG 0.689160 3.2:+90401209 MS.gene42251:CDS
ATGCTTGTAGTTCCACAAGG+TGG 0.691545 3.2:+90399585 MS.gene42251:CDS
TACGCGTAGCTATACGCGCG+TGG 0.692773 3.2:-90399825 None:intergenic
AGTCTTGTGAACAGAGAGTG+CGG 0.693380 3.2:-90399398 None:intergenic
AATTCTGCTAAGGTAGCTGG+TGG 0.728419 3.2:-90399495 None:intergenic
TCATCGCCTCAGCTTAACAA+CGG 0.733250 3.2:+90401136 MS.gene42251:CDS
GGATTGAAGAACGAAACCGG+TGG 0.773497 3.2:-90399322 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAATTAAATAAAACAAT+TGG + chr3.2:90400419-90400438 MS.gene42251:intron 10.0%
!!! AATTGTTTTATTTAATTCTA+TGG - chr3.2:90400420-90400439 None:intergenic 10.0%
!!! ATTGATAGATTTTGTATAAT+TGG - chr3.2:90400386-90400405 None:intergenic 15.0%
!!! TAATTGGTTTTATAAAGTAT+AGG - chr3.2:90400370-90400389 None:intergenic 15.0%
!! CTTAAAAATGAGTTTATAGA+GGG - chr3.2:90400956-90400975 None:intergenic 20.0%
!!! TTAGTCAAATTTCACTTTTT+GGG + chr3.2:90400713-90400732 MS.gene42251:intron 20.0%
!!! TTTAGTCAAATTTCACTTTT+TGG + chr3.2:90400712-90400731 MS.gene42251:intron 20.0%
!!! TTTTTTCTTATAAATGTGAC+CGG - chr3.2:90400195-90400214 None:intergenic 20.0%
! ACTACTAAAATAATCACCTT+GGG - chr3.2:90399929-90399948 None:intergenic 25.0%
! ATACTTAATCTTTCAGTATC+AGG + chr3.2:90399207-90399226 MS.gene42251:CDS 25.0%
! ATGTATCTAGTCACATTTAT+AGG - chr3.2:90400256-90400275 None:intergenic 25.0%
! CCATATAATCAATTGAGATT+CGG - chr3.2:90400625-90400644 None:intergenic 25.0%
! CTGAAAGATTAAGTATGATA+AGG - chr3.2:90399203-90399222 None:intergenic 25.0%
! GCTTAAAAATGAGTTTATAG+AGG - chr3.2:90400957-90400976 None:intergenic 25.0%
! TACTACTAAAATAATCACCT+TGG - chr3.2:90399930-90399949 None:intergenic 25.0%
! TACTTAATCTTTCAGTATCA+GGG + chr3.2:90399208-90399227 MS.gene42251:CDS 25.0%
! TTACAGAAAGAAAAGATTCT+TGG - chr3.2:90401032-90401051 None:intergenic 25.0%
!! AATAGAGTACTAAGTAGTAA+CGG - chr3.2:90400086-90400105 None:intergenic 25.0%
!! AGAACTTGAGAGATTTTATT+AGG + chr3.2:90400840-90400859 MS.gene42251:intron 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG + chr3.2:90400304-90400323 MS.gene42251:intron 25.0%
!!! TTGGTTTTATAAAGTATAGG+TGG - chr3.2:90400367-90400386 None:intergenic 25.0%
AACTATTCTTACCATCACAA+CGG + chr3.2:90401240-90401259 MS.gene42251:CDS 30.0%
ACTTAATCTTTCAGTATCAG+GGG + chr3.2:90399209-90399228 MS.gene42251:CDS 30.0%
CATTATCAATTAAGCACATG+TGG + chr3.2:90400672-90400691 MS.gene42251:intron 30.0%
CCGAATCTCAATTGATTATA+TGG + chr3.2:90400622-90400641 MS.gene42251:intron 30.0%
TGATCAGAGTGAAATATCAT+GGG + chr3.2:90401189-90401208 MS.gene42251:CDS 30.0%
TGTGATGGTAAGAATAGTTT+GGG - chr3.2:90401239-90401258 None:intergenic 30.0%
TTGATCAGAGTGAAATATCA+TGG + chr3.2:90401188-90401207 MS.gene42251:CDS 30.0%
TTGTGATGGTAAGAATAGTT+TGG - chr3.2:90401240-90401259 None:intergenic 30.0%
TTTGAATGAAGTATGAACGT+TGG + chr3.2:90400032-90400051 MS.gene42251:intron 30.0%
! CAATCAATTGGAGAGTATAA+AGG - chr3.2:90400595-90400614 None:intergenic 30.0%
!! GGATTAGAATTTTAGCCTTT+AGG + chr3.2:90400643-90400662 MS.gene42251:intron 30.0%
!! TTAGAATTTTAGCCTTTAGG+TGG + chr3.2:90400646-90400665 MS.gene42251:intron 30.0%
!! TTGATAATGTATCCACCTAA+AGG - chr3.2:90400661-90400680 None:intergenic 30.0%
!!! TTTCACTTTTTGGGATATCA+CGG + chr3.2:90400722-90400741 MS.gene42251:intron 30.0%
AACAACTCAGAATAACTCGA+AGG - chr3.2:90399792-90399811 None:intergenic 35.0%
ACAAATGCAACTACACAGTA+TGG + chr3.2:90399259-90399278 MS.gene42251:CDS 35.0%
GATTCTTGGCGTACATTATT+TGG - chr3.2:90401018-90401037 None:intergenic 35.0%
TACAAATCACATACTTCCTC+CGG - chr3.2:90400326-90400345 None:intergenic 35.0%
TACTAAGTAGTAACGGTTAG+CGG - chr3.2:90400079-90400098 None:intergenic 35.0%
TCTCCTATAGTCCTATGTAT+GGG + chr3.2:90400499-90400518 MS.gene42251:intron 35.0%
TTAGCAGAATTCACTCTTGA+CGG + chr3.2:90399507-90399526 MS.gene42251:CDS 35.0%
TTCTTATAAATGTGACCGGA+TGG - chr3.2:90400191-90400210 None:intergenic 35.0%
! TTCGGTGTACTTCAATCAAT+TGG - chr3.2:90400607-90400626 None:intergenic 35.0%
AACTCTATGCTTAGCTCGAA+GGG + chr3.2:90400442-90400461 MS.gene42251:intron 40.0%
CAAAATCTGGTCCCATACAT+AGG - chr3.2:90400513-90400532 None:intergenic 40.0%
CCTTGCCTTTCTTCAAAATC+TGG - chr3.2:90400526-90400545 None:intergenic 40.0%
CTCGAAGGTTTACAAGTGTT+AGG - chr3.2:90399777-90399796 None:intergenic 40.0%
GGAAAATTCTCCTGTCTTAC+CGG + chr3.2:90399426-90399445 MS.gene42251:CDS 40.0%
GGACAAAATCCGAAATCAGA+TGG + chr3.2:90401103-90401122 MS.gene42251:CDS 40.0%
GGTATTCGTACAGTTATCAC+CGG - chr3.2:90399613-90399632 None:intergenic 40.0%
GGTGATAACTGTACGAATAC+CGG + chr3.2:90399612-90399631 MS.gene42251:CDS 40.0%
GGTTTATAATGCACTGCATC+TGG + chr3.2:90400476-90400495 MS.gene42251:intron 40.0%
GTCAAGAGTGAATTCTGCTA+AGG - chr3.2:90399508-90399527 None:intergenic 40.0%
GTCTCCTATAGTCCTATGTA+TGG + chr3.2:90400498-90400517 MS.gene42251:intron 40.0%
TAACTATTCCGTACTCCATC+CGG + chr3.2:90400173-90400192 MS.gene42251:intron 40.0%
TGAAGAAAGGCAAGGAAAAG+TGG + chr3.2:90400531-90400550 MS.gene42251:intron 40.0%
TTCGACGTGAGTTTAGTTGA+CGG + chr3.2:90399549-90399568 MS.gene42251:CDS 40.0%
TTGCTTATAAATGTGACCGG+AGG + chr3.2:90400307-90400326 MS.gene42251:intron 40.0%
TTTCCACACATTGATACACG+TGG - chr3.2:90401215-90401234 None:intergenic 40.0%
! GGTAAGACAGGAGAATTTTC+CGG - chr3.2:90399427-90399446 None:intergenic 40.0%
! GTTTTTGCAGTCAAAAGTCG+TGG + chr3.2:90401077-90401096 MS.gene42251:intron 40.0%
! TACGAATACCGGTTGTATCA+GGG + chr3.2:90399623-90399642 MS.gene42251:CDS 40.0%
!! CCAGATTTTGAAGAAAGGCA+AGG + chr3.2:90400523-90400542 MS.gene42251:intron 40.0%
!! TGGGACCAGATTTTGAAGAA+AGG + chr3.2:90400518-90400537 MS.gene42251:intron 40.0%
AAATGTGACCGGATGGAGTA+CGG - chr3.2:90400184-90400203 None:intergenic 45.0%
AATTCTGCTAAGGTAGCTGG+TGG - chr3.2:90399498-90399517 None:intergenic 45.0%
ACGGCACAATATTGTTGCAG+CGG + chr3.2:90399738-90399757 MS.gene42251:CDS 45.0%
ATGCAACTACACAGTATGGC+CGG + chr3.2:90399263-90399282 MS.gene42251:CDS 45.0%
ATGCTTGTAGTTCCACAAGG+TGG + chr3.2:90399585-90399604 MS.gene42251:CDS 45.0%
CACGTTTACATGTGCTTCTG+CGG + chr3.2:90399863-90399882 MS.gene42251:CDS 45.0%
CCAATGCTTGTAGTTCCACA+AGG + chr3.2:90399582-90399601 MS.gene42251:CDS 45.0%
CCTTGTGGAACTACAAGCAT+TGG - chr3.2:90399585-90399604 None:intergenic 45.0%
CTTACCGGTGATTGTAACTC+CGG + chr3.2:90399441-90399460 MS.gene42251:CDS 45.0%
GAACTCTATGCTTAGCTCGA+AGG + chr3.2:90400441-90400460 MS.gene42251:intron 45.0%
GAGCAAATCCCTGATACAAC+CGG - chr3.2:90399634-90399653 None:intergenic 45.0%
GCTCGAAGGGCTACTAATAA+TGG + chr3.2:90400455-90400474 MS.gene42251:intron 45.0%
GGTCCCATACATAGGACTAT+AGG - chr3.2:90400505-90400524 None:intergenic 45.0%
GTGAATTCTGCTAAGGTAGC+TGG - chr3.2:90399501-90399520 None:intergenic 45.0%
GTTACAATCACCGGTAAGAC+AGG - chr3.2:90399439-90399458 None:intergenic 45.0%
TCATCGCCTCAGCTTAACAA+CGG + chr3.2:90401136-90401155 MS.gene42251:CDS 45.0%
TGTTAAGCTGAGGCGATGAT+GGG - chr3.2:90401135-90401154 None:intergenic 45.0%
TTAAGGTTACGAGCGTGGAT+GGG + chr3.2:90399673-90399692 MS.gene42251:CDS 45.0%
TTACCATCACAACGGCTATG+TGG + chr3.2:90401248-90401267 MS.gene42251:CDS 45.0%
TTGTTAAGCTGAGGCGATGA+TGG - chr3.2:90401136-90401155 None:intergenic 45.0%
TTTGCCGGAGTTACAATCAC+CGG - chr3.2:90399448-90399467 None:intergenic 45.0%
! AAGACAGGAGAATTTTCCGG+TGG - chr3.2:90399424-90399443 None:intergenic 45.0%
! ACCGGTTTCGTTCTTCAATC+CGG + chr3.2:90399324-90399343 MS.gene42251:CDS 45.0%
! AGTCTTGTGAACAGAGAGTG+CGG - chr3.2:90399401-90399420 None:intergenic 45.0%
! CTCGTAACCTTAAGCTCTGA+AGG - chr3.2:90399666-90399685 None:intergenic 45.0%
! GAATTCACTCTTGACGGATC+CGG + chr3.2:90399513-90399532 MS.gene42251:CDS 45.0%
! GGTTGTATCAGGGATTTGCT+CGG + chr3.2:90399633-90399652 MS.gene42251:CDS 45.0%
! GTACGAATACCGGTTGTATC+AGG + chr3.2:90399622-90399641 MS.gene42251:CDS 45.0%
!! AAAAGTGCTTGTGAGGCGTT+TGG + chr3.2:90399714-90399733 MS.gene42251:CDS 45.0%
!!! TAATTTAATGTTTTAAAAAA+CGG - chr3.2:90400155-90400174 None:intergenic 5.0%
AAAGTCGAATGCTCCGGCAA+CGG + chr3.2:90399465-90399484 MS.gene42251:CDS 50.0%
AAGCCACATAGCCGTTGTGA+TGG - chr3.2:90401254-90401273 None:intergenic 50.0%
AGAGCTTAAGGTTACGAGCG+TGG + chr3.2:90399668-90399687 MS.gene42251:CDS 50.0%
CTCTGTTCACAAGACTCCAC+CGG + chr3.2:90399405-90399424 MS.gene42251:CDS 50.0%
CTTAAGGTTACGAGCGTGGA+TGG + chr3.2:90399672-90399691 MS.gene42251:CDS 50.0%
GCCGGATTGAAGAACGAAAC+CGG - chr3.2:90399328-90399347 None:intergenic 50.0%
GGATTGAAGAACGAAACCGG+TGG - chr3.2:90399325-90399344 None:intergenic 50.0%
GGGTCACTGCCATCTGATTT+CGG - chr3.2:90401115-90401134 None:intergenic 50.0%
GTCGAAGAAATCGAGTCCAC+CGG - chr3.2:90399535-90399554 None:intergenic 50.0%
TCACCTTGGGCTGTTGTTAG+TGG - chr3.2:90399916-90399935 None:intergenic 50.0%
TGAAGAACGAAACCGGTGGT+TGG - chr3.2:90399321-90399340 None:intergenic 50.0%
TGCAACTACACAGTATGGCC+GGG + chr3.2:90399264-90399283 MS.gene42251:CDS 50.0%
TGCAGTCAAAAGTCGTGGCA+GGG + chr3.2:90401082-90401101 MS.gene42251:intron 50.0%
TTGCAGTCAAAAGTCGTGGC+AGG + chr3.2:90401081-90401100 MS.gene42251:intron 50.0%
! TAACAACGGCTCTGGCTCAA+TGG + chr3.2:90401150-90401169 MS.gene42251:CDS 50.0%
! TCTGGCTCAATGGTGTACGT+TGG + chr3.2:90401160-90401179 MS.gene42251:CDS 50.0%
! TGCTTGTGAGGCGTTTGGAA+CGG + chr3.2:90399719-90399738 MS.gene42251:CDS 50.0%
!! GGCGTGTAAAAGTGCTTGTG+AGG + chr3.2:90399707-90399726 MS.gene42251:CDS 50.0%
!! GGTGATTGATTTGGAGTCGC+CGG - chr3.2:90399346-90399365 None:intergenic 50.0%
ACGAAACCGGTGGTTGGAAG+CGG - chr3.2:90399315-90399334 None:intergenic 55.0%
AGCGGGTGGGGTGATTGATT+TGG - chr3.2:90399355-90399374 None:intergenic 55.0%
CCAGAGCCGTTGTTAAGCTG+AGG - chr3.2:90401145-90401164 None:intergenic 55.0%
CCTCAGCTTAACAACGGCTC+TGG + chr3.2:90401142-90401161 MS.gene42251:CDS 55.0%
CCTCCACTAACAACAGCCCA+AGG + chr3.2:90399910-90399929 MS.gene42251:CDS 55.0%
CCTTGGGCTGTTGTTAGTGG+AGG - chr3.2:90399913-90399932 None:intergenic 55.0%
CGCGTGTCCTTCAGAGCTTA+AGG + chr3.2:90399656-90399675 MS.gene42251:CDS 55.0%
GGCTCAATGGTGTACGTTGG+TGG + chr3.2:90401163-90401182 MS.gene42251:CDS 55.0%
GGGCCACGTGTATCAATGTG+TGG + chr3.2:90401209-90401228 MS.gene42251:CDS 55.0%
GTAGTTCCACAAGGTGGCTC+CGG + chr3.2:90399591-90399610 MS.gene42251:CDS 55.0%
TATTGTTGCAGCGGCGCGTA+TGG + chr3.2:90399747-90399766 MS.gene42251:CDS 55.0%
TCCGGCAAAGTCGAATGCTC+CGG + chr3.2:90399459-90399478 MS.gene42251:CDS 55.0%
TTATCACCGGAGCCACCTTG+TGG - chr3.2:90399600-90399619 None:intergenic 55.0%
! TTCACTCTTGACGGATCCGG+TGG + chr3.2:90399516-90399535 MS.gene42251:CDS 55.0%
AATCACCCCACCCGCTTCCT+GGG + chr3.2:90399359-90399378 MS.gene42251:CDS 60.0%
CCGGGAATTCTCTCCAACGC+CGG + chr3.2:90399282-90399301 MS.gene42251:CDS 60.0%
CGTGGATGGGAAAGAGAGCG+TGG + chr3.2:90399686-90399705 MS.gene42251:CDS 60.0%
GTTGCACCGCTTCCAACCAC+CGG + chr3.2:90399306-90399325 MS.gene42251:CDS 60.0%
TACGCGTAGCTATACGCGCG+TGG - chr3.2:90399828-90399847 None:intergenic 60.0%
TTCCTGGGGAGGCCGTTTCT+GGG + chr3.2:90399374-90399393 MS.gene42251:CDS 60.0%
! AGAGAGTGCGGCCCCAGAAA+CGG - chr3.2:90399389-90399408 None:intergenic 60.0%
! CCGGCGTTGGAGAGAATTCC+CGG - chr3.2:90399285-90399304 None:intergenic 60.0%
! GCCGGAGCATTCGACTTTGC+CGG - chr3.2:90399463-90399482 None:intergenic 60.0%
AAACGGCCTCCCCAGGAAGC+GGG - chr3.2:90399372-90399391 None:intergenic 65.0%
ATCACCCCACCCGCTTCCTG+GGG + chr3.2:90399360-90399379 MS.gene42251:CDS 65.0%
CAATCACCCCACCCGCTTCC+TGG + chr3.2:90399358-90399377 MS.gene42251:CDS 65.0%
CTTCCTGGGGAGGCCGTTTC+TGG + chr3.2:90399373-90399392 MS.gene42251:CDS 65.0%
GAAACGGCCTCCCCAGGAAG+CGG - chr3.2:90399373-90399392 None:intergenic 65.0%
GGTTGGAAGCGGTGCAACGC+CGG - chr3.2:90399304-90399323 None:intergenic 65.0%
TCCTGGGGAGGCCGTTTCTG+GGG + chr3.2:90399375-90399394 MS.gene42251:CDS 65.0%
!! TGGTGGTGTTGCGCCGTTGC+CGG - chr3.2:90399481-90399500 None:intergenic 65.0%
AAGCGGTGCAACGCCGGCGT+TGG - chr3.2:90399298-90399317 None:intergenic 70.0%
ACCCCACCCGCTTCCTGGGG+AGG + chr3.2:90399363-90399382 MS.gene42251:CDS 75.0%
GCCCCAGAAACGGCCTCCCC+AGG - chr3.2:90399379-90399398 None:intergenic 75.0%
GCCTCCCCAGGAAGCGGGTG+GGG - chr3.2:90399367-90399386 None:intergenic 75.0%
GGCCTCCCCAGGAAGCGGGT+GGG - chr3.2:90399368-90399387 None:intergenic 75.0%
! CGGCCTCCCCAGGAAGCGGG+TGG - chr3.2:90399369-90399388 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 90399165 90401297 90399165 ID=MS.gene42251
chr3.2 mRNA 90399165 90401297 90399165 ID=MS.gene42251.t1;Parent=MS.gene42251
chr3.2 exon 90399165 90399931 90399165 ID=MS.gene42251.t1.exon1;Parent=MS.gene42251.t1
chr3.2 CDS 90399165 90399931 90399165 ID=cds.MS.gene42251.t1;Parent=MS.gene42251.t1
chr3.2 exon 90401087 90401297 90401087 ID=MS.gene42251.t1.exon2;Parent=MS.gene42251.t1
chr3.2 CDS 90401087 90401297 90401087 ID=cds.MS.gene42251.t1;Parent=MS.gene42251.t1
Gene Sequence

>MS.gene42251

ATGGATCAACTAAGTACTAAATTATTTTCAATTTTCCTTATCATACTTAATCTTTCAGTATCAGGGGTATTATCAACAACATTCACACTCGTTAACAAATGCAACTACACAGTATGGCCGGGAATTCTCTCCAACGCCGGCGTTGCACCGCTTCCAACCACCGGTTTCGTTCTTCAATCCGGCGACTCCAAATCAATCACCCCACCCGCTTCCTGGGGAGGCCGTTTCTGGGGCCGCACTCTCTGTTCACAAGACTCCACCGGAAAATTCTCCTGTCTTACCGGTGATTGTAACTCCGGCAAAGTCGAATGCTCCGGCAACGGCGCAACACCACCAGCTACCTTAGCAGAATTCACTCTTGACGGATCCGGTGGACTCGATTTCTTCGACGTGAGTTTAGTTGACGGTTACAACGTTCCAATGCTTGTAGTTCCACAAGGTGGCTCCGGTGATAACTGTACGAATACCGGTTGTATCAGGGATTTGCTCGGCGCGTGTCCTTCAGAGCTTAAGGTTACGAGCGTGGATGGGAAAGAGAGCGTGGCGTGTAAAAGTGCTTGTGAGGCGTTTGGAACGGCACAATATTGTTGCAGCGGCGCGTATGGTTCACCTAACACTTGTAAACCTTCGAGTTATTCTGAGTTGTTTAAAAGTGCTTGTCCACGCGCGTATAGCTACGCGTACGACGATAAGACAAGCACGTTTACATGTGCTTCTGCGGCTGAATACACAATCACTTTTTGCTCCTCCACTAACAACAGCCCAAGGTGATTATTTTAGTAGTATTTATTTACTTTTATTTAATTTTTCACATACTTACTTAAATGATTTTTATTTACTTTACTTTGACGTTAGTAAATTTTTTTTTTTGAATGAAGTATGAACGTTGGATGCAGAAATGATTGAGATTGCCGCTAACCGTTACTACTTAGTACTCTATTAGTCTTACGTTACAATTTTTTACTTCTAGAGCGACAAAATTACTAACCGTTTTTTAAAACATTAAATTAACTATTCCGTACTCCATCCGGTCACATTTATAAGAAAAAAACAAATTTTTAGATACATTGAATAATTAATGTATCTAACCTATAAATGTGACTAGATACATTAATTATTCAATGTATCTAAAAAATCATTTTTTGCTTATAAATGTGACCGGAGGAAGTATGTGATTTGTAATGTCAAAACTTCTTGTTCCACCTATACTTTATAAAACCAATTATACAAAATCTATCAATTATCAGTGCTACCATAGAATTAAATAAAACAATTGGAACTCTATGCTTAGCTCGAAGGGCTACTAATAATGGTTTATAATGCACTGCATCTGGTCTCCTATAGTCCTATGTATGGGACCAGATTTTGAAGAAAGGCAAGGAAAAGTGGTTTATACTTAATTTCTTTTTTTTTTGTTAAAAAGAAGTCCTTTATACTCTCCAATTGATTGAAGTACACCGAATCTCAATTGATTATATGGATTAGAATTTTAGCCTTTAGGTGGATACATTATCAATTAAGCACATGTGGTACACAGTACACTCATCTTTAGTCAAATTTCACTTTTTGGGATATCACGGCTAGTGATCTGATTTAATAACTTTTTTTACATGAATTGACGCTAAACTAGTAGTACAATTCAAGAATCAAGATAGAAAAAAAAAATGTTCATACTAGAACTTGAGAGATTTTATTAGGTTTTTTTAATGCTACTCTTTCTAAACTCACTTCACTCTTGTTAAAATCTTGATGTTGAACTTAAATTATCTTGTAAATGAGATGAAATGTCCCTCTATAAACTCATTTTTAAGCTGATTTCAAGTTTTATTATACACGAGTCTTGAGTTTTTCCAAATAATGTACGCCAAGAATCTTTTCTTTCTGTAATGCAAATTAATGTTGTTGATAATTTGTTTTTGCAGTCAAAAGTCGTGGCAGGGACAAAATCCGAAATCAGATGGCAGTGACCCATCATCGCCTCAGCTTAACAACGGCTCTGGCTCAATGGTGTACGTTGGTGGCTTTGATCAGAGTGAAATATCATGGGCCACGTGTATCAATGTGTGGAAATCCCAAACTATTCTTACCATCACAACGGCTATGTGGCTTCTGTGTCAGCTTTTCCACTTCTAA
Protein sequence

>MS.gene42251.t1

MDQLSTKLFSIFLIILNLSVSGVLSTTFTLVNKCNYTVWPGILSNAGVAPLPTTGFVLQSGDSKSITPPASWGGRFWGRTLCSQDSTGKFSCLTGDCNSGKVECSGNGATPPATLAEFTLDGSGGLDFFDVSLVDGYNVPMLVVPQGGSGDNCTNTGCIRDLLGACPSELKVTSVDGKESVACKSACEAFGTAQYCCSGAYGSPNTCKPSSYSELFKSACPRAYSYAYDDKTSTFTCASAAEYTITFCSSTNNSPSQKSWQGQNPKSDGSDPSSPQLNNGSGSMVYVGGFDQSEISWATCINVWKSQTILTITTAMWLLCQLFHF