AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27493

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27493.t1 MTR_7g073430 92.226 283 14 2 1 275 1 283 0.0 538
MS.gene27493.t1 MTR_7g073380 81.973 294 34 4 1 275 1 294 2.03e-175 485
MS.gene27493.t1 MTR_0002s1250 43.952 248 121 7 12 253 5 240 2.48e-62 197
MS.gene27493.t1 MTR_8g005750 50.485 206 87 5 15 218 6 198 1.90e-60 193
MS.gene27493.t1 MTR_8g032510 46.948 213 102 5 13 218 7 215 3.30e-56 182
MS.gene27493.t1 MTR_2g075690 38.783 263 124 8 1 229 1 260 2.22e-47 160
MS.gene27493.t1 MTR_2g075700 39.130 253 125 5 6 229 7 259 2.80e-43 149
MS.gene27493.t1 MTR_2g075680 38.372 258 122 7 10 231 12 268 1.30e-38 137
MS.gene27493.t1 MTR_5g067700 38.362 232 109 13 1 228 1 202 2.33e-37 132
MS.gene27493.t1 MTR_3g093830 43.386 189 84 9 20 195 23 201 4.60e-35 128
MS.gene27493.t1 MTR_2g075750 55.446 101 44 1 106 205 13 113 6.33e-35 124
MS.gene27493.t1 MTR_2g075610 47.727 132 62 3 106 232 14 143 2.04e-34 123
MS.gene27493.t1 MTR_7g117200 34.848 198 100 5 11 194 9 191 8.87e-28 109
MS.gene27493.t1 MTR_3g090860 33.498 203 102 4 9 185 2 197 3.01e-26 105
MS.gene27493.t1 MTR_8g099350 33.663 202 101 7 1 180 1 191 7.73e-24 99.4
MS.gene27493.t1 MTR_8g027860 52.941 85 32 1 104 180 111 195 2.92e-23 97.4
MS.gene27493.t1 MTR_5g067680 41.667 108 57 2 74 180 159 261 2.65e-19 86.7
MS.gene27493.t1 MTR_7g028415 55.932 59 23 1 121 179 93 148 6.06e-16 73.9
MS.gene27493.t1 MTR_7g028710 55.932 59 23 1 121 179 93 148 6.06e-16 73.9
MS.gene27493.t1 MTR_5g073620 42.667 75 42 1 117 190 668 742 6.23e-15 75.1
MS.gene27493.t1 MTR_8g092140 42.169 83 38 3 96 178 142 214 6.29e-14 69.7
MS.gene27493.t1 MTR_5g043880 38.710 93 53 2 121 212 124 213 1.19e-13 69.7
MS.gene27493.t1 MTR_1g086790 50.847 59 26 1 121 179 158 213 1.65e-13 69.7
MS.gene27493.t1 MTR_7g110720 39.316 117 65 3 65 178 158 271 2.07e-13 70.1
MS.gene27493.t1 MTR_7g079010 50.000 58 26 1 121 178 141 195 2.89e-13 67.8
MS.gene27493.t1 MTR_3g095080 49.153 59 27 1 121 179 168 223 3.44e-13 67.8
MS.gene27493.t1 MTR_3g095080 49.153 59 27 1 121 179 144 199 4.95e-13 67.0
MS.gene27493.t1 MTR_7g109800 50.847 59 26 1 121 179 145 200 5.27e-13 68.2
MS.gene27493.t1 MTR_2g088000 36.449 107 65 2 96 202 325 428 6.67e-13 68.9
MS.gene27493.t1 MTR_8g096730 48.276 58 27 1 121 178 70 124 6.93e-13 65.1
MS.gene27493.t1 MTR_8g028565 44.872 78 39 2 104 179 136 211 1.10e-12 67.4
MS.gene27493.t1 MTR_4g094588 51.724 58 25 1 121 178 178 232 1.24e-12 67.4
MS.gene27493.t1 MTR_3g053330 38.384 99 58 2 104 202 235 330 1.62e-12 67.4
MS.gene27493.t1 MTR_5g091390 50.000 58 26 1 121 178 157 211 1.79e-12 66.6
MS.gene27493.t1 MTR_8g087000 36.893 103 53 2 115 217 71 161 2.02e-12 65.9
MS.gene27493.t1 MTR_8g087000 36.893 103 53 2 115 217 70 160 2.20e-12 65.9
MS.gene27493.t1 MTR_7g110720 46.053 76 38 2 103 178 172 244 5.68e-12 65.9
MS.gene27493.t1 MTR_8g007270 43.529 85 43 2 94 178 129 208 5.70e-12 64.3
MS.gene27493.t1 MTR_4g049790 33.846 130 75 3 77 206 277 395 6.31e-12 65.9
MS.gene27493.t1 MTR_1g009613 36.842 95 56 3 85 178 226 317 6.77e-12 65.1
MS.gene27493.t1 MTR_2g033820 47.458 59 28 1 121 179 203 258 7.21e-12 65.5
MS.gene27493.t1 MTR_2g032030 35.185 108 56 2 83 178 217 322 8.57e-12 65.1
MS.gene27493.t1 MTR_4g122530 44.828 58 29 1 121 178 208 262 9.83e-12 65.1
MS.gene27493.t1 MTR_2g032030 35.185 108 56 2 83 178 217 322 1.05e-11 65.1
MS.gene27493.t1 MTR_7g062220 51.724 58 25 1 121 178 124 178 1.87e-11 63.9
MS.gene27493.t1 MTR_3g104750 47.458 59 28 1 121 179 104 159 1.98e-11 61.6
MS.gene27493.t1 MTR_4g127040 40.000 85 40 3 102 178 219 300 2.11e-11 63.5
MS.gene27493.t1 MTR_4g082580 48.276 58 27 1 121 178 278 332 2.21e-11 63.9
MS.gene27493.t1 MTR_2g023930 38.384 99 50 2 80 178 273 360 2.98e-11 63.9
MS.gene27493.t1 MTR_1g015140 32.800 125 68 4 66 179 38 157 3.79e-11 60.8
MS.gene27493.t1 MTR_2g016770 39.785 93 52 3 87 178 1 90 6.36e-11 61.2
MS.gene27493.t1 MTR_2g016770 40.741 81 45 2 99 178 4 82 8.60e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27493.t1 AT3G56400 41.436 181 82 4 13 180 10 179 1.75e-38 137
MS.gene27493.t1 AT2G40750 35.885 209 102 8 3 180 4 211 1.29e-26 106
MS.gene27493.t1 AT1G66550 34.653 202 110 7 13 210 8 191 3.68e-26 103
MS.gene27493.t1 AT2G46400 31.301 246 116 8 14 234 8 225 1.04e-25 103
MS.gene27493.t1 AT1G66550 33.000 200 111 6 13 210 8 186 3.91e-25 100
MS.gene27493.t1 AT5G01900 34.896 192 103 5 13 197 7 183 4.29e-25 100
MS.gene27493.t1 AT5G22570 34.637 179 98 5 13 185 9 174 3.54e-24 99.0
MS.gene27493.t1 AT4G11070 36.041 197 95 6 11 180 8 200 1.36e-23 97.8
MS.gene27493.t1 AT1G66560 34.706 170 96 4 15 183 10 165 1.53e-23 96.7
MS.gene27493.t1 AT4G11070 37.079 178 87 5 11 180 8 168 1.98e-22 94.4
MS.gene27493.t1 AT2G40740 31.414 191 104 5 15 180 44 232 9.52e-22 92.4
MS.gene27493.t1 AT2G40740 31.414 191 104 5 15 180 53 241 1.06e-21 92.4
MS.gene27493.t1 AT1G66600 33.889 180 88 7 13 183 8 165 2.79e-21 90.1
MS.gene27493.t1 AT4G23810 49.412 85 35 1 104 180 133 217 1.41e-19 87.0
MS.gene27493.t1 AT1G80590 29.648 199 102 5 12 208 7 169 3.26e-19 84.3
MS.gene27493.t1 AT5G24110 33.146 178 92 4 23 185 12 177 1.18e-18 84.3
MS.gene27493.t1 AT5G45260 40.000 105 58 2 79 178 1161 1265 2.49e-15 76.3
MS.gene27493.t1 AT1G69310 38.017 121 57 5 68 178 89 201 3.81e-15 74.3
MS.gene27493.t1 AT1G69310 38.017 121 57 5 68 178 89 201 3.81e-15 74.3
MS.gene27493.t1 AT1G69310 38.017 121 57 5 68 178 89 201 3.81e-15 74.3
MS.gene27493.t1 AT5G13080 47.761 67 32 1 121 187 67 130 1.37e-13 67.0
MS.gene27493.t1 AT5G41570 51.724 58 25 1 121 178 98 152 1.54e-13 67.8
MS.gene27493.t1 AT4G04450 37.719 114 68 2 90 202 166 277 1.57e-13 70.5
MS.gene27493.t1 AT2G34830 35.246 122 65 3 62 178 167 279 2.18e-13 70.1
MS.gene27493.t1 AT2G34830 35.246 122 65 3 62 178 158 270 2.20e-13 70.1
MS.gene27493.t1 AT4G04450 37.719 114 68 2 90 202 260 371 2.30e-13 70.1
MS.gene27493.t1 AT5G49520 49.153 59 27 1 121 179 221 276 3.30e-13 69.3
MS.gene27493.t1 AT3G01970 42.045 88 40 2 121 208 65 141 3.69e-13 65.9
MS.gene27493.t1 AT1G64000 50.000 58 26 1 121 178 114 168 7.44e-13 66.2
MS.gene27493.t1 AT1G29860 42.308 78 41 2 106 182 120 194 8.39e-13 67.4
MS.gene27493.t1 AT2G47260 37.634 93 54 2 88 179 140 229 8.44e-13 67.8
MS.gene27493.t1 AT4G18170 42.169 83 44 2 104 185 154 233 1.29e-12 67.4
MS.gene27493.t1 AT2G37260 46.970 66 32 1 121 186 349 411 1.68e-12 67.4
MS.gene27493.t1 AT2G37260 46.970 66 32 1 121 186 267 329 2.09e-12 66.6
MS.gene27493.t1 AT2G37260 46.970 66 32 1 121 186 269 331 2.15e-12 66.6
MS.gene27493.t1 AT4G39410 40.000 90 49 2 89 178 193 277 2.22e-12 66.2
MS.gene27493.t1 AT2G23320 38.462 104 53 3 83 178 195 295 2.22e-12 66.6
MS.gene27493.t1 AT5G46350 46.154 65 32 1 121 185 183 244 2.48e-12 66.6
MS.gene27493.t1 AT2G46130 46.552 58 28 1 121 178 30 84 2.68e-12 62.4
MS.gene27493.t1 AT2G44745 48.276 58 27 1 121 178 145 199 4.22e-12 64.7
MS.gene27493.t1 AT1G62300 48.649 74 35 2 106 178 296 367 4.57e-12 66.2
MS.gene27493.t1 AT5G52830 38.596 114 54 4 126 235 170 271 5.70e-12 65.5
MS.gene27493.t1 AT4G01250 36.364 110 62 3 71 178 80 183 9.05e-12 64.7
MS.gene27493.t1 AT1G29280 46.667 60 30 1 119 178 72 129 1.25e-11 63.9
MS.gene27493.t1 AT1G68150 34.307 137 76 4 122 250 236 366 1.73e-11 64.3
MS.gene27493.t1 AT3G62340 43.590 78 36 2 121 198 118 187 2.29e-11 63.2
MS.gene27493.t1 AT5G26170 44.828 58 29 1 121 178 113 167 3.56e-11 61.2
MS.gene27493.t1 AT4G22070 54.386 57 24 1 122 178 298 352 3.63e-11 63.5
MS.gene27493.t1 AT4G22070 54.386 57 24 1 122 178 298 352 3.63e-11 63.5
MS.gene27493.t1 AT4G22070 54.386 57 24 1 122 178 298 352 3.63e-11 63.5
MS.gene27493.t1 AT4G22070 54.386 57 24 1 122 178 298 352 3.63e-11 63.5
MS.gene27493.t1 AT5G45050 35.185 108 56 3 81 178 993 1096 7.30e-11 63.2
MS.gene27493.t1 AT5G45050 35.185 108 56 3 81 178 1132 1235 7.35e-11 63.2
MS.gene27493.t1 AT5G45050 35.185 108 56 3 81 178 1104 1207 8.49e-11 62.8
MS.gene27493.t1 AT1G30650 46.774 62 31 1 117 178 213 272 8.63e-11 62.4

Find 58 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATAATCCACCATGATTTC+AGG 0.223815 7.2:-31205550 None:intergenic
GTGGATCTTCTTGAATTCTT+TGG 0.233438 7.2:-31205474 None:intergenic
TTTAAGAAAGAGGCCAATTA+AGG 0.247289 7.2:-31205801 None:intergenic
CACCAAATCTGAAGACTCTT+TGG 0.292085 7.2:+31203776 MS.gene27493:CDS
CATACATGATATCAGAAATA+TGG 0.313913 7.2:-31205771 None:intergenic
CTTCACAAGACAACCTATTC+TGG 0.318721 7.2:+31205499 MS.gene27493:CDS
TTCCAAAGAGTCTTCAGATT+TGG 0.321094 7.2:-31203778 None:intergenic
GGTTATTGTTCAAGAACAAT+AGG 0.331883 7.2:-31203725 None:intergenic
TAAGTTGGTTTGCAAGTTGA+TGG 0.334142 7.2:-31203599 None:intergenic
TGTATGATGATGTCCTTAAT+TGG 0.354460 7.2:+31205788 MS.gene27493:CDS
TTGCAAGAGTTCCATTGTTA+AGG 0.357033 7.2:+31203806 MS.gene27493:CDS
ATAAATATACCTTGGAAAAT+CGG 0.383422 7.2:-31204631 None:intergenic
GGTCATCAGTGGAGAAAGTA+TGG 0.398881 7.2:+31204588 MS.gene27493:CDS
CATTAAGAATATTTCTAAGT+TGG 0.405861 7.2:-31203614 None:intergenic
TTCAAAAGATCTTCAGCAAA+TGG 0.413114 7.2:-31203675 None:intergenic
AGAAATATTCTTAATGAAAG+TGG 0.420955 7.2:+31203621 MS.gene27493:CDS
GAGAAAAGGACAGTCTTGTT+TGG 0.421010 7.2:-31205626 None:intergenic
GTTATTGTTCAAGAACAATA+GGG 0.426826 7.2:-31203724 None:intergenic
CAGTCTTGTTTGGCTGGTGT+TGG 0.428837 7.2:-31205616 None:intergenic
ATAGTTGATGAGTTGTTAAG+AGG 0.433990 7.2:+31203576 MS.gene27493:CDS
GCAAGATTCTACAAGTATGA+TGG 0.441895 7.2:-31205522 None:intergenic
GATAATCCACCATGATTTCA+GGG 0.452507 7.2:-31205549 None:intergenic
AGTCTTGTTTGGCTGGTGTT+GGG 0.452762 7.2:-31205615 None:intergenic
CTTGCAAAACCCTGAAATCA+TGG 0.466864 7.2:+31205540 MS.gene27493:CDS
AAAGGACAGTCTTGTTTGGC+TGG 0.470880 7.2:-31205622 None:intergenic
GATATTGTTCATCCACTCTC+TGG 0.472425 7.2:+31205676 MS.gene27493:CDS
CACTGATGACCATCTTCCTC+AGG 0.473789 7.2:-31204576 None:intergenic
AATCTAGCTCATGGTGGTCG+TGG 0.489503 7.2:+31203549 MS.gene27493:CDS
CAGGAAAATTTCACAAACAT+GGG 0.503777 7.2:+31204536 MS.gene27493:intron
ACAAGTATGATGGCCAGAAT+AGG 0.508994 7.2:-31205512 None:intergenic
AAATCAGAGCCCTCAGAAGT+AGG 0.513880 7.2:-31203747 None:intergenic
TCTGAGCTGCCTGAGGAAGA+TGG 0.519536 7.2:+31204567 MS.gene27493:CDS
ACAGGAAAATTTCACAAACA+TGG 0.526761 7.2:+31204535 MS.gene27493:intron
AATCAGAGCCCTCAGAAGTA+GGG 0.528391 7.2:-31203746 None:intergenic
GAACAATAGGGAATTAGTAA+AGG 0.532955 7.2:-31203712 None:intergenic
GAAGAGGCTGTTACAAAAGA+AGG 0.533769 7.2:+31203832 MS.gene27493:CDS
GGTGATATTGATAATAATGA+TGG 0.535663 7.2:+31203642 MS.gene27493:CDS
GAAAATGAAAATCTAGCTCA+TGG 0.549115 7.2:+31203540 MS.gene27493:CDS
AACAATAACCCTACTTCTGA+GGG 0.553155 7.2:+31203738 MS.gene27493:CDS
GAACAATAACCCTACTTCTG+AGG 0.557215 7.2:+31203737 MS.gene27493:CDS
GCCTCTTCGTTCCTTAACAA+TGG 0.560157 7.2:-31203817 None:intergenic
CAATAGGGAATTAGTAAAGG+TGG 0.561256 7.2:-31203709 None:intergenic
TGCACTCACAAACATGATCA+AGG 0.563442 7.2:+31205430 MS.gene27493:CDS
TGCTTTGCAGAAACTACTAC+AGG 0.577466 7.2:+31205407 MS.gene27493:intron
AAGAAACACGGAAGAGTGAT+CGG 0.578189 7.2:-31205575 None:intergenic
TAGGTTGTCTTGTGAAGAGG+TGG 0.580386 7.2:-31205493 None:intergenic
GAAGGAGTCTGAGCTGCCTG+AGG 0.592747 7.2:+31204560 MS.gene27493:CDS
AATTTCACAAACATGGGAGA+AGG 0.598123 7.2:+31204542 MS.gene27493:CDS
GAATAGGTTGTCTTGTGAAG+AGG 0.606524 7.2:-31205496 None:intergenic
TCAAAAGTGAGTCCAGAGAG+TGG 0.622190 7.2:-31205688 None:intergenic
TATCAGAAATATGGACACCT+AGG 0.634267 7.2:-31205762 None:intergenic
TCCATTGTTAAGGAACGAAG+AGG 0.645691 7.2:+31203816 MS.gene27493:CDS
AACACGGAAGAGTGATCGGT+AGG 0.657967 7.2:-31205571 None:intergenic
CTTGATTCTCATGATCACCT+AGG 0.662285 7.2:+31205745 MS.gene27493:CDS
CTGAGGAAGATGGTCATCAG+TGG 0.677795 7.2:+31204577 MS.gene27493:CDS
GTTGTCAAAACTAAGAAACA+CGG 0.709857 7.2:-31205587 None:intergenic
AATGAAAATCTAGCTCATGG+TGG 0.721998 7.2:+31203543 MS.gene27493:CDS
GCAAAACCCTGAAATCATGG+TGG 0.724951 7.2:+31205543 MS.gene27493:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAAAAAA+AGG - chr7.2:31204101-31204120 None:intergenic 0.0%
!!! AACTAAATCTTATTTATTAA+AGG - chr7.2:31204959-31204978 None:intergenic 10.0%
!! AAAATTCAGGTATTAATTTA+AGG + chr7.2:31205015-31205034 MS.gene27493:intron 15.0%
!! GTTAGTTAATTTATTGAATT+AGG - chr7.2:31204741-31204760 None:intergenic 15.0%
!!! GTTTTTTTTTTTGTTACAAT+TGG + chr7.2:31204478-31204497 MS.gene27493:intron 15.0%
!!! TGTTTTGAAAATTAATTAAC+CGG - chr7.2:31205129-31205148 None:intergenic 15.0%
!!! TTTACTATATAGTTTTGTTT+AGG + chr7.2:31204060-31204079 MS.gene27493:intron 15.0%
!! AAGGACTATGTTATATAATT+AGG + chr7.2:31204323-31204342 MS.gene27493:intron 20.0%
!! AATTATATAACATAGTCCTT+GGG - chr7.2:31204323-31204342 None:intergenic 20.0%
!! AGAAATATTCTTAATGAAAG+TGG + chr7.2:31203621-31203640 MS.gene27493:CDS 20.0%
!! ATAAATATACCTTGGAAAAT+CGG - chr7.2:31204634-31204653 None:intergenic 20.0%
!! ATATACCGATATAGATATAA+TGG - chr7.2:31204009-31204028 None:intergenic 20.0%
!! TAATTATATAACATAGTCCT+TGG - chr7.2:31204324-31204343 None:intergenic 20.0%
!! TACATTAGTCAATAAATAAG+AGG - chr7.2:31204161-31204180 None:intergenic 20.0%
!! TATACCGATATAGATATAAT+GGG - chr7.2:31204008-31204027 None:intergenic 20.0%
!!! CATTAAGAATATTTCTAAGT+TGG - chr7.2:31203617-31203636 None:intergenic 20.0%
!!! CTTTTACATATGAGTAATAT+TGG + chr7.2:31204295-31204314 MS.gene27493:intron 20.0%
!!! TATATACAATTCTTGTTTAC+AGG + chr7.2:31204517-31204536 MS.gene27493:intron 20.0%
!!! TTCACTTATATTTGAACTTT+TGG + chr7.2:31204183-31204202 MS.gene27493:intron 20.0%
!!! TTTCTAAAGTTTGTTAGTTA+TGG + chr7.2:31204371-31204390 MS.gene27493:intron 20.0%
! AAAAGAAGTCAACTCTTAAA+TGG - chr7.2:31203936-31203955 None:intergenic 25.0%
! AAACGAGCAAATTAATAAAG+AGG - chr7.2:31204354-31204373 None:intergenic 25.0%
! AAATTGTAGATGTAGAAATG+TGG - chr7.2:31204032-31204051 None:intergenic 25.0%
! AATTGAATGACCTTGTAAAA+AGG - chr7.2:31205081-31205100 None:intergenic 25.0%
! ATTATATAACATAGTCCTTG+GGG - chr7.2:31204322-31204341 None:intergenic 25.0%
! ATTTATTAGCTAGCATCTTA+GGG + chr7.2:31205231-31205250 MS.gene27493:intron 25.0%
! CATACATGATATCAGAAATA+TGG - chr7.2:31205774-31205793 None:intergenic 25.0%
! GTTATTGTTCAAGAACAATA+GGG - chr7.2:31203727-31203746 None:intergenic 25.0%
! TATTTATTAGCTAGCATCTT+AGG + chr7.2:31205230-31205249 MS.gene27493:intron 25.0%
!! GGTGATATTGATAATAATGA+TGG + chr7.2:31203642-31203661 MS.gene27493:CDS 25.0%
!! TATATTTGAACTTTTGGATG+AGG + chr7.2:31204189-31204208 MS.gene27493:intron 25.0%
!! TATTGATTAGTAAGAAGTAG+AGG - chr7.2:31205363-31205382 None:intergenic 25.0%
ACAGGAAAATTTCACAAACA+TGG + chr7.2:31204535-31204554 MS.gene27493:intron 30.0%
CACTATTTGTACAAATTGTC+CGG + chr7.2:31205107-31205126 MS.gene27493:intron 30.0%
CAGGAAAATTTCACAAACAT+GGG + chr7.2:31204536-31204555 MS.gene27493:intron 30.0%
CTCTCCCATTATATCTATAT+CGG + chr7.2:31204001-31204020 MS.gene27493:intron 30.0%
GAACAATAGGGAATTAGTAA+AGG - chr7.2:31203715-31203734 None:intergenic 30.0%
GGTTATTGTTCAAGAACAAT+AGG - chr7.2:31203728-31203747 None:intergenic 30.0%
GTTGTCAAAACTAAGAAACA+CGG - chr7.2:31205590-31205609 None:intergenic 30.0%
TAGAAACTCCCTTGTAAAAA+AGG - chr7.2:31204678-31204697 None:intergenic 30.0%
TGTGTCGTATAAATATACCT+TGG - chr7.2:31204642-31204661 None:intergenic 30.0%
TTAACTGTTGAGATTTGACT+TGG + chr7.2:31205166-31205185 MS.gene27493:intron 30.0%
TTCAAAAGATCTTCAGCAAA+TGG - chr7.2:31203678-31203697 None:intergenic 30.0%
! ATAGATATAATGGGAGAGTT+AGG - chr7.2:31203999-31204018 None:intergenic 30.0%
! CTAGAAATGACCTTTTTACA+AGG + chr7.2:31205068-31205087 MS.gene27493:intron 30.0%
! GAAAATGAAAATCTAGCTCA+TGG + chr7.2:31203540-31203559 MS.gene27493:CDS 30.0%
! TAGATATAATGGGAGAGTTA+GGG - chr7.2:31203998-31204017 None:intergenic 30.0%
! TGTATGATGATGTCCTTAAT+TGG + chr7.2:31205788-31205807 MS.gene27493:CDS 30.0%
!! ATAGTTGATGAGTTGTTAAG+AGG + chr7.2:31203576-31203595 MS.gene27493:CDS 30.0%
!! ATTTGCTGAAGATCTTTTGA+AGG + chr7.2:31203677-31203696 MS.gene27493:CDS 30.0%
!! TTTTTCAAACACACTGCTTA+GGG + chr7.2:31203965-31203984 MS.gene27493:intron 30.0%
!!! TTTTTTCAAACACACTGCTT+AGG + chr7.2:31203964-31203983 MS.gene27493:intron 30.0%
AACAATAACCCTACTTCTGA+GGG + chr7.2:31203738-31203757 MS.gene27493:CDS 35.0%
AATTTCACAAACATGGGAGA+AGG + chr7.2:31204542-31204561 MS.gene27493:CDS 35.0%
CAATAGGGAATTAGTAAAGG+TGG - chr7.2:31203712-31203731 None:intergenic 35.0%
CACACACACATATCATTTCT+TGG + chr7.2:31204451-31204470 MS.gene27493:intron 35.0%
CCTTGTAAAAAAGGTTTGTG+CGG - chr7.2:31204669-31204688 None:intergenic 35.0%
GATAATCCACCATGATTTCA+GGG - chr7.2:31205552-31205571 None:intergenic 35.0%
GCAAGATTCTACAAGTATGA+TGG - chr7.2:31205525-31205544 None:intergenic 35.0%
GTGGATCTTCTTGAATTCTT+TGG - chr7.2:31205477-31205496 None:intergenic 35.0%
TAACCTTTGACCTAAATCAC+TGG - chr7.2:31205267-31205286 None:intergenic 35.0%
TATCAGAAATATGGACACCT+AGG - chr7.2:31205765-31205784 None:intergenic 35.0%
TGAACTGAAAGAGTTTCTGA+TGG + chr7.2:31205289-31205308 MS.gene27493:intron 35.0%
TTCCAAAGAGTCTTCAGATT+TGG - chr7.2:31203781-31203800 None:intergenic 35.0%
TTGCAAGAGTTCCATTGTTA+AGG + chr7.2:31203806-31203825 MS.gene27493:CDS 35.0%
TTGTTAGTTATGGTTTGAGC+TGG + chr7.2:31204381-31204400 MS.gene27493:intron 35.0%
! AATGAAAATCTAGCTCATGG+TGG + chr7.2:31203543-31203562 MS.gene27493:CDS 35.0%
! TCCTTTTCTCTCAGTGAAAA+AGG + chr7.2:31205639-31205658 MS.gene27493:CDS 35.0%
! TTTTCTCTCAGTGAAAAAGG+AGG + chr7.2:31205642-31205661 MS.gene27493:CDS 35.0%
!! CACTTTTGATGATCCAGAAA+AGG + chr7.2:31205702-31205721 MS.gene27493:CDS 35.0%
!! GCAGCAGCAAAAGTATTAAT+TGG - chr7.2:31204256-31204275 None:intergenic 35.0%
!! TAAGTTGGTTTGCAAGTTGA+TGG - chr7.2:31203602-31203621 None:intergenic 35.0%
!! TCCTTTTTCACTGAGAGAAA+AGG - chr7.2:31205643-31205662 None:intergenic 35.0%
!!! CGCACAAACCTTTTTTACAA+GGG + chr7.2:31204667-31204686 MS.gene27493:intron 35.0%
!!! CGGTGTGCAAAATCTTTTTT+TGG - chr7.2:31204614-31204633 None:intergenic 35.0%
AAGAAACACGGAAGAGTGAT+CGG - chr7.2:31205578-31205597 None:intergenic 40.0%
ACAAGTATGATGGCCAGAAT+AGG - chr7.2:31205515-31205534 None:intergenic 40.0%
ATGAGTAATATTGGACCCCA+AGG + chr7.2:31204304-31204323 MS.gene27493:intron 40.0%
CACCAAATCTGAAGACTCTT+TGG + chr7.2:31203776-31203795 MS.gene27493:CDS 40.0%
CTTCACAAGACAACCTATTC+TGG + chr7.2:31205499-31205518 MS.gene27493:CDS 40.0%
CTTGATTCTCATGATCACCT+AGG + chr7.2:31205745-31205764 MS.gene27493:CDS 40.0%
CTTGCAAAACCCTGAAATCA+TGG + chr7.2:31205540-31205559 MS.gene27493:CDS 40.0%
GAACAATAACCCTACTTCTG+AGG + chr7.2:31203737-31203756 MS.gene27493:CDS 40.0%
GAAGAGGCTGTTACAAAAGA+AGG + chr7.2:31203832-31203851 MS.gene27493:CDS 40.0%
GAATAGGTTGTCTTGTGAAG+AGG - chr7.2:31205499-31205518 None:intergenic 40.0%
GATATTGTTCATCCACTCTC+TGG + chr7.2:31205676-31205695 MS.gene27493:CDS 40.0%
GGATAATCCACCATGATTTC+AGG - chr7.2:31205553-31205572 None:intergenic 40.0%
TCCATTGTTAAGGAACGAAG+AGG + chr7.2:31203816-31203835 MS.gene27493:CDS 40.0%
TGCACTCACAAACATGATCA+AGG + chr7.2:31205430-31205449 MS.gene27493:CDS 40.0%
TGCTTTGCAGAAACTACTAC+AGG + chr7.2:31205407-31205426 MS.gene27493:intron 40.0%
TTGAGTAGGTCCAGTGATTT+AGG + chr7.2:31205254-31205273 MS.gene27493:intron 40.0%
! GATAGTGTGACATCCTTTTC+TGG - chr7.2:31205718-31205737 None:intergenic 40.0%
!! GAGAAAAGGACAGTCTTGTT+TGG - chr7.2:31205629-31205648 None:intergenic 40.0%
!!! CCGCACAAACCTTTTTTACA+AGG + chr7.2:31204666-31204685 MS.gene27493:intron 40.0%
!!! TTTTGCACACCGATTTTCCA+AGG + chr7.2:31204622-31204641 MS.gene27493:CDS 40.0%
AAATCAGAGCCCTCAGAAGT+AGG - chr7.2:31203750-31203769 None:intergenic 45.0%
AATCAGAGCCCTCAGAAGTA+GGG - chr7.2:31203749-31203768 None:intergenic 45.0%
CTAGCATCTTAGGGTTGAGT+AGG + chr7.2:31205240-31205259 MS.gene27493:intron 45.0%
GCAAAACCCTGAAATCATGG+TGG + chr7.2:31205543-31205562 MS.gene27493:CDS 45.0%
GCCTCTTCGTTCCTTAACAA+TGG - chr7.2:31203820-31203839 None:intergenic 45.0%
GGTCATCAGTGGAGAAAGTA+TGG + chr7.2:31204588-31204607 MS.gene27493:CDS 45.0%
GGTCCAGTGATTTAGGTCAA+AGG + chr7.2:31205261-31205280 MS.gene27493:intron 45.0%
TAGGTTGTCTTGTGAAGAGG+TGG - chr7.2:31205496-31205515 None:intergenic 45.0%
TCAAAAGTGAGTCCAGAGAG+TGG - chr7.2:31205691-31205710 None:intergenic 45.0%
! AGTGAAAAAGGAGGAGTGCA+AGG + chr7.2:31205651-31205670 MS.gene27493:CDS 45.0%
!! AAAGGACAGTCTTGTTTGGC+TGG - chr7.2:31205625-31205644 None:intergenic 45.0%
!! AGTCTTGTTTGGCTGGTGTT+GGG - chr7.2:31205618-31205637 None:intergenic 45.0%
!! ATAATTTAAAAAAAAAATTC+AGG + chr7.2:31205002-31205021 MS.gene27493:intron 5.0%
!!! TTTAAGAAAATATTTTATTT+TGG + chr7.2:31204120-31204139 MS.gene27493:intron 5.0%
AACACGGAAGAGTGATCGGT+AGG - chr7.2:31205574-31205593 None:intergenic 50.0%
CACTGATGACCATCTTCCTC+AGG - chr7.2:31204579-31204598 None:intergenic 50.0%
CTGAGGAAGATGGTCATCAG+TGG + chr7.2:31204577-31204596 MS.gene27493:CDS 50.0%
! AATCTAGCTCATGGTGGTCG+TGG + chr7.2:31203549-31203568 MS.gene27493:CDS 50.0%
!! CAGTCTTGTTTGGCTGGTGT+TGG - chr7.2:31205619-31205638 None:intergenic 50.0%
TCTGAGCTGCCTGAGGAAGA+TGG + chr7.2:31204567-31204586 MS.gene27493:CDS 55.0%
GAAGGAGTCTGAGCTGCCTG+AGG + chr7.2:31204560-31204579 MS.gene27493:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 31203537 31205822 31203537 ID=MS.gene27493
chr7.2 mRNA 31203537 31205822 31203537 ID=MS.gene27493.t1;Parent=MS.gene27493
chr7.2 exon 31203537 31203853 31203537 ID=MS.gene27493.t1.exon1;Parent=MS.gene27493.t1
chr7.2 CDS 31203537 31203853 31203537 ID=cds.MS.gene27493.t1;Parent=MS.gene27493.t1
chr7.2 exon 31204539 31204643 31204539 ID=MS.gene27493.t1.exon2;Parent=MS.gene27493.t1
chr7.2 CDS 31204539 31204643 31204539 ID=cds.MS.gene27493.t1;Parent=MS.gene27493.t1
chr7.2 exon 31205417 31205822 31205417 ID=MS.gene27493.t1.exon3;Parent=MS.gene27493.t1
chr7.2 CDS 31205417 31205822 31205417 ID=cds.MS.gene27493.t1;Parent=MS.gene27493.t1
Gene Sequence

>MS.gene27493

ATGGAAAATGAAAATCTAGCTCATGGTGGTCGTGGAAAGATAGTTGATGAGTTGTTAAGAGGCCATCAACTTGCAAACCAACTTAGAAATATTCTTAATGAAAGTGGTGATATTGATAATAATGATGGATCAACAATACCATTTGCTGAAGATCTTTTGAAGGAAGTGCTTACCACCTTTACTAATTCCCTATTGTTCTTGAACAATAACCCTACTTCTGAGGGCTCTGATTTGCAACTCACCAAATCTGAAGACTCTTTGGAAAGTAATTGCAAGAGTTCCATTGTTAAGGAACGAAGAGGCTGTTACAAAAGAAGGTAATTATTATTACTTAGTAATTTTTCTTATATTTTTTATTGTTTTATAAATCTTTGTATATGTGTGCGCATCTGAGATCCATTTAAGAGTTGACTTCTTTTAACATATATTTTTTCAAACACACTGCTTAGGGATTGAATCCCTAACTCTCCCATTATATCTATATCGGTATATCCACATTTCTACATCTACAATTTGTACATGATTTACTATATAGTTTTGTTTAGGAATATAGTGCTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTAAGAAAATATTTTATTTTGGCTGATATATTTTTATCCTCTTATTTATTGACTAATGTAATTTCACTTATATTTGAACTTTTGGATGAGGTAATGTATGCTTTGTTATTTAATTTGATTACAGACTATTAGCCAATTAATACTTTTGCTGCTGCTGCTGCTGCTGCTGTTTGTCTTTTACATATGAGTAATATTGGACCCCAAGGACTATGTTATATAATTAGGAACGTCCTCTTTATTAATTTGCTCGTTTCTAAAGTTTGTTAGTTATGGTTTGAGCTGGCACAGTAAAATTATTTGATTATATATATGTGTCTATACACACACACACACACACACATATCATTTCTTGGTTGAGTTTTTTTTTTTGTTACAATTGGAAAACTTATCAGAAAATATATACAATTCTTGTTTACAGGAAAATTTCACAAACATGGGAGAAGGAGTCTGAGCTGCCTGAGGAAGATGGTCATCAGTGGAGAAAGTATGGCCAAAAAAAGATTTTGCACACCGATTTTCCAAGGTATATTTATACGACACATTAACCGCACAAACCTTTTTTACAAGGGAGTTTCTACTACACACTTGTAAATTGATGTGTATCGATGCACTTGTTTCCTAATTCAATAAATTAACTAACATTTTTATTAATTAAAAAAGTTATTTGTCGATTTTATATTTTAGAAAACACTATTTCATAAGAAAAAAAATATCATTTCATTGTTTATTAAGAATCATACTGAATATTTTACATTTTATAGCAAAAATAAATAAATCAGTATTCACTATTATGAGTAATAAATATTTAAAACAATAAAATTTTATTTCATTGAAACCTTTAATAAATAAGATTTAGTTATTATAATTGTAAATGATAGAAAATAATTTAAAAAAAAAATTCAGGTATTAATTTAAGGACTCAATGCACTCGAATTTTCAAGTGTAAACTAGAAATGACCTTTTTACAAGGTCATTCAATTAAATTTCACTATTTGTACAAATTGTCCGGTTAATTAATTTTCAAAACAATTAATCTAAATTATCATTAACTGTTGAGATTTGACTTGGTTTAATTTATGTATGTCTCTTATATCTGTTTATATGAAATTTATTTATTAGCTAGCATCTTAGGGTTGAGTAGGTCCAGTGATTTAGGTCAAAGGTTAATTGAACTGAAAGAGTTTCTGATGGATAAATAGACATGACAACTAATTAATATTTGTTAATACAAACAAAAATCCTCTACTTCTTACTAATCAATATCACTAAATGAATATGTACTTATGTGCTTTGCAGAAACTACTACAGGTGCACTCACAAACATGATCAAGGTTGCAAAGCAACAAAACAAGTCCAAAGAATTCAAGAAGATCCACCTCTTCACAAGACAACCTATTCTGGCCATCATACTTGTAGAATCTTGCAAAACCCTGAAATCATGGTGGATTATCCTACCGATCACTCTTCCGTGTTTCTTAGTTTTGACAACTCTTTCCCAACACCAGCCAAACAAGACTGTCCTTTTCTCTCAGTGAAAAAGGAGGAGTGCAAGGAAGATATTGTTCATCCACTCTCTGGACTCACTTTTGATGATCCAGAAAAGGATGTCACACTATCATCAACACTTGATTCTCATGATCACCTAGGTGTCCATATTTCTGATATCATGTATGATGATGTCCTTAATTGGCCTCTTTCTTAA
Protein sequence

>MS.gene27493.t1

MENENLAHGGRGKIVDELLRGHQLANQLRNILNESGDIDNNDGSTIPFAEDLLKEVLTTFTNSLLFLNNNPTSEGSDLQLTKSEDSLESNCKSSIVKERRGCYKRRKISQTWEKESELPEEDGHQWRKYGQKKILHTDFPRNYYRCTHKHDQGCKATKQVQRIQEDPPLHKTTYSGHHTCRILQNPEIMVDYPTDHSSVFLSFDNSFPTPAKQDCPFLSVKKEECKEDIVHPLSGLTFDDPEKDVTLSSTLDSHDHLGVHISDIMYDDVLNWPLS