AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28792


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28792.t1 MTR_7g109250 96.942 327 10 0 1 327 1 327 0.0 661
MS.gene28792.t1 MTR_7g109250 96.942 327 10 0 1 327 61 387 0.0 658
MS.gene28792.t1 MTR_7g108868 51.412 177 26 5 62 238 11 127 1.36e-46 156
MS.gene28792.t1 MTR_8g058593 51.412 177 26 5 62 238 11 127 1.36e-46 156
MS.gene28792.t1 MTR_8g058607 50.000 176 28 4 62 237 24 139 1.16e-45 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28792.t1 AT1G01350 69.375 320 95 2 8 327 20 336 3.31e-163 459
MS.gene28792.t1 AT5G06420 68.125 320 96 2 8 327 58 371 3.17e-161 455
MS.gene28792.t1 AT5G06420 68.125 320 96 2 8 327 58 371 3.17e-161 455
MS.gene28792.t1 AT4G01023 64.407 59 21 0 258 316 76 134 2.53e-26 104
MS.gene28792.t1 AT4G01023 65.079 63 21 1 254 316 92 153 5.86e-26 104
MS.gene28792.t1 AT4G01023 64.407 59 21 0 258 316 95 153 8.26e-26 103

Find 66 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATATTCTTCCTATTAAC+AGG 0.250339 7.4:+6084711 None:intergenic
TCTTCAGAAAACCTGTTAAT+AGG 0.277151 7.4:-6084722 MS.gene28792:CDS
TTGTAATCCTTGCATATATC+TGG 0.290645 7.4:+6084204 None:intergenic
GTGGGTCACACGGTCCTCTC+AGG 0.313412 7.4:-6084269 MS.gene28792:CDS
GATCGAGGTGATTACAAATC+TGG 0.331028 7.4:-6084135 MS.gene28792:CDS
TGCAAGGATTACAAGGAAAC+TGG 0.409524 7.4:-6084195 MS.gene28792:CDS
AAATGAAAGCTGGGTTTCTC+AGG 0.415855 7.4:+6084534 None:intergenic
CATTTGTCGAAACCCATTTG+TGG 0.418726 7.4:-6083974 MS.gene28792:CDS
CTTGCTTAAGAGCTCTTTCA+CGG 0.431115 7.4:+6084418 None:intergenic
CTGTGAGCATTGTGCATTGA+AGG 0.436410 7.4:-6083920 MS.gene28792:intron
TAGCAAAGCAACAGCAACTT+TGG 0.436558 7.4:-6084478 MS.gene28792:CDS
GTTGAGACAGGATCCACAAA+TGG 0.441292 7.4:+6083961 None:intergenic
AGAAGACTCGAAATGAAAGC+TGG 0.441893 7.4:+6084524 None:intergenic
ACACGGTCCTCTCAGGGCTT+CGG 0.450938 7.4:-6084262 MS.gene28792:CDS
TGCTTACACTTGGTTGAGAC+AGG 0.460245 7.4:+6083949 None:intergenic
GTCTGCAATCAGCCTACGCT+TGG 0.462383 7.4:-6083752 MS.gene28792:CDS
CAATAATTATACAGATCATA+AGG 0.469416 7.4:-6084334 MS.gene28792:CDS
GGTGGCAGATGGAGAAAGAA+TGG 0.469953 7.4:-6084113 MS.gene28792:CDS
TGCAGGTGAGGATGCGGAAG+AGG 0.471533 7.4:-6084055 MS.gene28792:CDS
CGAGGATGTAAAATTATACA+AGG 0.486690 7.4:-6084361 MS.gene28792:CDS
ACATACCTTGTTCAGTCGGC+TGG 0.489348 7.4:+6084860 None:intergenic
GCAAACAATTGCCAGTGAAA+AGG 0.499122 7.4:-6084295 MS.gene28792:CDS
CGACAAATGAAACACGCAAA+TGG 0.499537 7.4:+6083988 None:intergenic
GAAAGCAAGGAAGATGAGAT+TGG 0.507679 7.4:-6084079 MS.gene28792:CDS
TGCAGAGCTCTTCGAAGAGC+CGG 0.511210 7.4:+6084578 None:intergenic
AATTATACAGATCATAAGGC+TGG 0.520545 7.4:-6084330 MS.gene28792:CDS
ATCGAGGTGATTACAAATCT+GGG 0.520829 7.4:-6084134 MS.gene28792:CDS
GAGCTCTTTCACGGATGGCA+CGG 0.524231 7.4:+6084427 None:intergenic
TTGAGACAGGATCCACAAAT+GGG 0.525917 7.4:+6083962 None:intergenic
AGATATATGCAAGGATTACA+AGG 0.530308 7.4:-6084202 MS.gene28792:CDS
TGGGTCACACGGTCCTCTCA+GGG 0.532282 7.4:-6084268 MS.gene28792:CDS
CTTAAGAGCTCTTTCACGGA+TGG 0.538525 7.4:+6084422 None:intergenic
AGTCTCAAGGGAAAGAGTAC+TGG 0.538575 7.4:-6084387 MS.gene28792:CDS
GAAAACCAGCCGACTGAACA+AGG 0.548242 7.4:-6084865 MS.gene28792:intron
GTGGCAGATGGAGAAAGAAT+GGG 0.553122 7.4:-6084112 MS.gene28792:CDS
TTACAAATCTGGGTGGCAGA+TGG 0.553483 7.4:-6084124 MS.gene28792:CDS
GAAGACTCGAAATGAAAGCT+GGG 0.554193 7.4:+6084525 None:intergenic
AGCTCTTTCACGGATGGCAC+GGG 0.560125 7.4:+6084428 None:intergenic
GAAACTGGTTACTGTGGGTA+TGG 0.560524 7.4:-6084180 MS.gene28792:CDS
TATTGAGAATGAAGATAATG+AGG 0.560776 7.4:-6084679 MS.gene28792:CDS
TCATGAGATACGAAAGAAGA+TGG 0.563924 7.4:-6083714 MS.gene28792:CDS
ATAGGAAGAATATGAGGAAG+CGG 0.564756 7.4:-6084704 MS.gene28792:CDS
GGTGAGGATGCGGAAGAGGA+AGG 0.573050 7.4:-6084051 MS.gene28792:CDS
CTGTTAATAGGAAGAATATG+AGG 0.583376 7.4:-6084710 MS.gene28792:CDS
GGGATGAAGCTGAGAAAGCA+AGG 0.584805 7.4:-6084092 MS.gene28792:CDS
TACAAGGAAACTGGTTACTG+TGG 0.587264 7.4:-6084186 MS.gene28792:CDS
ACAATTGCCAGTGAAAAGGC+TGG 0.588175 7.4:-6084291 MS.gene28792:CDS
TTATACATACCTTGTTCAGT+CGG 0.588673 7.4:+6084856 None:intergenic
GATGAGATTGGCTGCAGGTG+AGG 0.590274 7.4:-6084067 MS.gene28792:CDS
GAAAAGGCTGGTGGGTCACA+CGG 0.600903 7.4:-6084279 MS.gene28792:CDS
AGGAAGATGAGATTGGCTGC+AGG 0.603143 7.4:-6084072 MS.gene28792:CDS
ATTGGCTGCAGGTGAGGATG+CGG 0.603342 7.4:-6084061 MS.gene28792:CDS
ATTGCCAGTGAAAAGGCTGG+TGG 0.607353 7.4:-6084288 MS.gene28792:CDS
GATACGAAAGAAGATGGCTG+AGG 0.623889 7.4:-6083708 MS.gene28792:CDS
TTGCCAGTGAAAAGGCTGGT+GGG 0.630138 7.4:-6084287 MS.gene28792:CDS
CAAGCGACTGAGAGTCTCAA+GGG 0.630489 7.4:-6084399 MS.gene28792:CDS
ACAGAAGTAGTGCTTACACT+TGG 0.630927 7.4:+6083939 None:intergenic
GCAAGCGACTGAGAGTCTCA+AGG 0.636269 7.4:-6084400 MS.gene28792:CDS
GGGAAAGAGTACTGGTTCCG+AGG 0.640397 7.4:-6084379 MS.gene28792:CDS
ACAAGGAAACTGGTTACTGT+GGG 0.647981 7.4:-6084185 MS.gene28792:CDS
TGTAAGTTTATGCATGATCG+AGG 0.650874 7.4:-6084150 MS.gene28792:CDS
TTATCAGCCAGATATATGCA+AGG 0.656472 7.4:-6084211 MS.gene28792:CDS
AGAGCTCTTCGAAGAGCCGG+TGG 0.686462 7.4:+6084581 None:intergenic
GAGGATGTAAAATTATACAA+GGG 0.688297 7.4:-6084360 MS.gene28792:CDS
ATGTGAGCCGAAGCCCTGAG+AGG 0.689295 7.4:+6084255 None:intergenic
GAGGTGATTACAAATCTGGG+TGG 0.758440 7.4:-6084131 MS.gene28792:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAAAAGGTTAATGA+TGG + chr7.4:6083821-6083840 None:intergenic 20.0%
!! CAATAATTATACAGATCATA+AGG - chr7.4:6084258-6084277 MS.gene28792:CDS 20.0%
! AGAAGTTAGGCATAATTAAA+AGG + chr7.4:6084776-6084795 None:intergenic 25.0%
! GAGGATGTAAAATTATACAA+GGG - chr7.4:6084232-6084251 MS.gene28792:CDS 25.0%
! TATTGAGAATGAAGATAATG+AGG - chr7.4:6083913-6083932 MS.gene28792:intron 25.0%
!! CTTGTATAATTTTACATCCT+CGG + chr7.4:6084233-6084252 None:intergenic 25.0%
!!! ACTTATTTGCTTTGAAGTTA+AGG + chr7.4:6084703-6084722 None:intergenic 25.0%
!!! CTTATTTGCTTTGAAGTTAA+GGG + chr7.4:6084702-6084721 None:intergenic 25.0%
AAGTAAAATGAGCAGAAGTT+AGG + chr7.4:6084789-6084808 None:intergenic 30.0%
AATTATACAGATCATAAGGC+TGG - chr7.4:6084262-6084281 MS.gene28792:CDS 30.0%
ACAAACGCGTAGAAAAAAAA+AGG + chr7.4:6083831-6083850 None:intergenic 30.0%
AGATATATGCAAGGATTACA+AGG - chr7.4:6084390-6084409 MS.gene28792:CDS 30.0%
CGAGGATGTAAAATTATACA+AGG - chr7.4:6084231-6084250 MS.gene28792:CDS 30.0%
CTCATATTCTTCCTATTAAC+AGG + chr7.4:6083884-6083903 None:intergenic 30.0%
CTGTTAATAGGAAGAATATG+AGG - chr7.4:6083882-6083901 MS.gene28792:intron 30.0%
GATGGTACAAAAAAATGTAG+AGG + chr7.4:6083803-6083822 None:intergenic 30.0%
TCTTCAGAAAACCTGTTAAT+AGG - chr7.4:6083870-6083889 MS.gene28792:intron 30.0%
TGTTCAGAAAAAGAACATGA+AGG - chr7.4:6083967-6083986 MS.gene28792:CDS 30.0%
TTATACATACCTTGTTCAGT+CGG + chr7.4:6083739-6083758 None:intergenic 30.0%
TTGTAATCCTTGCATATATC+TGG + chr7.4:6084391-6084410 None:intergenic 30.0%
! AATAAGTTGTTCTTTTCCAC+CGG - chr7.4:6083995-6084014 MS.gene28792:CDS 30.0%
ACATTAAAAATGCCAAGCGT+AGG + chr7.4:6084855-6084874 None:intergenic 35.0%
ATAGGAAGAATATGAGGAAG+CGG - chr7.4:6083888-6083907 MS.gene28792:intron 35.0%
ATCGAGGTGATTACAAATCT+GGG - chr7.4:6084458-6084477 MS.gene28792:CDS 35.0%
TCATGAGATACGAAAGAAGA+TGG - chr7.4:6084878-6084897 MS.gene28792:CDS 35.0%
TGTAAGTTTATGCATGATCG+AGG - chr7.4:6084442-6084461 MS.gene28792:CDS 35.0%
TTATCAGCCAGATATATGCA+AGG - chr7.4:6084381-6084400 MS.gene28792:CDS 35.0%
! TACGCTTGGCATTTTTAATG+TGG - chr7.4:6084854-6084873 MS.gene28792:intron 35.0%
AAATGAAAGCTGGGTTTCTC+AGG + chr7.4:6084061-6084080 None:intergenic 40.0%
ACAAGGAAACTGGTTACTGT+GGG - chr7.4:6084407-6084426 MS.gene28792:CDS 40.0%
ACAGAAGTAGTGCTTACACT+TGG + chr7.4:6084656-6084675 None:intergenic 40.0%
AGAAGACTCGAAATGAAAGC+TGG + chr7.4:6084071-6084090 None:intergenic 40.0%
CATTTGTCGAAACCCATTTG+TGG - chr7.4:6084618-6084637 MS.gene28792:CDS 40.0%
CGACAAATGAAACACGCAAA+TGG + chr7.4:6084607-6084626 None:intergenic 40.0%
CTTGCTTAAGAGCTCTTTCA+CGG + chr7.4:6084177-6084196 None:intergenic 40.0%
GAAAGCAAGGAAGATGAGAT+TGG - chr7.4:6084513-6084532 MS.gene28792:CDS 40.0%
GAAGACTCGAAATGAAAGCT+GGG + chr7.4:6084070-6084089 None:intergenic 40.0%
GATCGAGGTGATTACAAATC+TGG - chr7.4:6084457-6084476 MS.gene28792:CDS 40.0%
GCAAACAATTGCCAGTGAAA+AGG - chr7.4:6084297-6084316 MS.gene28792:CDS 40.0%
TACAAGGAAACTGGTTACTG+TGG - chr7.4:6084406-6084425 MS.gene28792:CDS 40.0%
TAGCAAAGCAACAGCAACTT+TGG - chr7.4:6084114-6084133 MS.gene28792:CDS 40.0%
TGCAAGGATTACAAGGAAAC+TGG - chr7.4:6084397-6084416 MS.gene28792:CDS 40.0%
TTGAGACAGGATCCACAAAT+GGG + chr7.4:6084633-6084652 None:intergenic 40.0%
ACAATTGCCAGTGAAAAGGC+TGG - chr7.4:6084301-6084320 MS.gene28792:CDS 45.0%
CTTAAGAGCTCTTTCACGGA+TGG + chr7.4:6084173-6084192 None:intergenic 45.0%
GAAACTGGTTACTGTGGGTA+TGG - chr7.4:6084412-6084431 MS.gene28792:CDS 45.0%
GAGGTGATTACAAATCTGGG+TGG - chr7.4:6084461-6084480 MS.gene28792:CDS 45.0%
GATACGAAAGAAGATGGCTG+AGG - chr7.4:6084884-6084903 MS.gene28792:CDS 45.0%
GTGGCAGATGGAGAAAGAAT+GGG - chr7.4:6084480-6084499 MS.gene28792:CDS 45.0%
GTTGAGACAGGATCCACAAA+TGG + chr7.4:6084634-6084653 None:intergenic 45.0%
TGCTTACACTTGGTTGAGAC+AGG + chr7.4:6084646-6084665 None:intergenic 45.0%
TTACAAATCTGGGTGGCAGA+TGG - chr7.4:6084468-6084487 MS.gene28792:CDS 45.0%
! AGTCTCAAGGGAAAGAGTAC+TGG - chr7.4:6084205-6084224 MS.gene28792:CDS 45.0%
! CTGTGAGCATTGTGCATTGA+AGG - chr7.4:6084672-6084691 MS.gene28792:CDS 45.0%
ACATACCTTGTTCAGTCGGC+TGG + chr7.4:6083735-6083754 None:intergenic 50.0%
ATTGCCAGTGAAAAGGCTGG+TGG - chr7.4:6084304-6084323 MS.gene28792:CDS 50.0%
CAAGCGACTGAGAGTCTCAA+GGG - chr7.4:6084193-6084212 MS.gene28792:CDS 50.0%
GAAAACCAGCCGACTGAACA+AGG - chr7.4:6083727-6083746 MS.gene28792:CDS 50.0%
GGGATGAAGCTGAGAAAGCA+AGG - chr7.4:6084500-6084519 MS.gene28792:CDS 50.0%
GGTGGCAGATGGAGAAAGAA+TGG - chr7.4:6084479-6084498 MS.gene28792:CDS 50.0%
TTGCCAGTGAAAAGGCTGGT+GGG - chr7.4:6084305-6084324 MS.gene28792:CDS 50.0%
! AGGAAGATGAGATTGGCTGC+AGG - chr7.4:6084520-6084539 MS.gene28792:CDS 50.0%
!!! CGGCTGGTTTTCAGCAGATT+TGG + chr7.4:6083719-6083738 None:intergenic 50.0%
AGCTCTTTCACGGATGGCAC+GGG + chr7.4:6084167-6084186 None:intergenic 55.0%
ATTGGCTGCAGGTGAGGATG+CGG - chr7.4:6084531-6084550 MS.gene28792:CDS 55.0%
GAGCTCTTTCACGGATGGCA+CGG + chr7.4:6084168-6084187 None:intergenic 55.0%
GCAAGCGACTGAGAGTCTCA+AGG - chr7.4:6084192-6084211 MS.gene28792:CDS 55.0%
GTCTGCAATCAGCCTACGCT+TGG - chr7.4:6084840-6084859 MS.gene28792:intron 55.0%
TGCAGAGCTCTTCGAAGAGC+CGG + chr7.4:6084017-6084036 None:intergenic 55.0%
! GAAAAGGCTGGTGGGTCACA+CGG - chr7.4:6084313-6084332 MS.gene28792:CDS 55.0%
! GATGAGATTGGCTGCAGGTG+AGG - chr7.4:6084525-6084544 MS.gene28792:CDS 55.0%
! TGACCCACCAGCCTTTTCAC+TGG + chr7.4:6084311-6084330 None:intergenic 55.0%
!! GGGAAAGAGTACTGGTTCCG+AGG - chr7.4:6084213-6084232 MS.gene28792:CDS 55.0%
ACACGGTCCTCTCAGGGCTT+CGG - chr7.4:6084330-6084349 MS.gene28792:CDS 60.0%
AGAGCTCTTCGAAGAGCCGG+TGG + chr7.4:6084014-6084033 None:intergenic 60.0%
ATGTGAGCCGAAGCCCTGAG+AGG + chr7.4:6084340-6084359 None:intergenic 60.0%
GGTGAGGATGCGGAAGAGGA+AGG - chr7.4:6084541-6084560 MS.gene28792:CDS 60.0%
TGCAGGTGAGGATGCGGAAG+AGG - chr7.4:6084537-6084556 MS.gene28792:CDS 60.0%
TGGGTCACACGGTCCTCTCA+GGG - chr7.4:6084324-6084343 MS.gene28792:CDS 60.0%
GTGGGTCACACGGTCCTCTC+AGG - chr7.4:6084323-6084342 MS.gene28792:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 6083700 6084914 6083700 ID=MS.gene28792
chr7.4 mRNA 6083700 6084914 6083700 ID=MS.gene28792.t1;Parent=MS.gene28792
chr7.4 exon 6084866 6084914 6084866 ID=MS.gene28792.t1.exon1;Parent=MS.gene28792.t1
chr7.4 CDS 6084866 6084914 6084866 ID=cds.MS.gene28792.t1;Parent=MS.gene28792.t1
chr7.4 exon 6083921 6084753 6083921 ID=MS.gene28792.t1.exon2;Parent=MS.gene28792.t1
chr7.4 CDS 6083921 6084753 6083921 ID=cds.MS.gene28792.t1;Parent=MS.gene28792.t1
chr7.4 exon 6083700 6083801 6083700 ID=MS.gene28792.t1.exon3;Parent=MS.gene28792.t1
chr7.4 CDS 6083700 6083801 6083700 ID=cds.MS.gene28792.t1;Parent=MS.gene28792.t1
Gene Sequence

>MS.gene28792

ATGGAAAATTCTGAGGCCAAATCTGCTGAAAACCAGCCGACTGAACAAGGTATGTATAATATTTTGCATTATTAGACTCACAATCTTGTTATCTTATGCACCTCTACATTTTTTTGTACCATCATTAACCTTTTTTTTTCTACGCGTTTGTTTATCGTCAGTGTGTAGTTTCTTCAGAAAACCTGTTAATAGGAAGAATATGAGGAAGCGGACTATTGAGAATGAAGATAATGAGGATGACTCGAACAATGAAGAGTCTTTGATGCATGTTCAGAAAAAGAACATGAAGGCTGATAATAAGTTGTTCTTTTCCACCGGCTCTTCGAAGAGCTCTGCATCTGCTAAACTGAATGAAGAACCTGAGAAACCCAGCTTTCATTTCGAGTCTTCTAAAGAGATTCAAGTTCAACATGATAGCAAAGCAACAGCAACTTTGGAGACTGAGACTGATTTTTCAAGAGATGCCCGTGCCATCCGTGAAAGAGCTCTTAAGCAAGCGACTGAGAGTCTCAAGGGAAAGAGTACTGGTTCCGAGGATGTAAAATTATACAAGGGAATCAATAATTATACAGATCATAAGGCTGGATTTCGTAGAGAGCAAACAATTGCCAGTGAAAAGGCTGGTGGGTCACACGGTCCTCTCAGGGCTTCGGCTCACATAAGAGTTTCAGCAAGATTTGATTATCAGCCAGATATATGCAAGGATTACAAGGAAACTGGTTACTGTGGGTATGGTGATTCGTGTAAGTTTATGCATGATCGAGGTGATTACAAATCTGGGTGGCAGATGGAGAAAGAATGGGATGAAGCTGAGAAAGCAAGGAAGATGAGATTGGCTGCAGGTGAGGATGCGGAAGAGGAAGGTGCTAGTTTGAATGATGAAGATGATGATGAAGACGTATTACCATTTGCGTGTTTCATTTGTCGAAACCCATTTGTGGATCCTGTCTCAACCAAGTGTAAGCACTACTTCTGTGAGCATTGTGCATTGAAGGTACACCCTTAACTTCAAAGCAAATAAGTTTTAATTTATAACAGTTTCAAGTTATTCAGTAATGCTTAATTTCTTTTTTCCTTTTAATTATGCCTAACTTCTGCTCATTTTACTTGCAGCATCATGCGAAGAATAAGAAGTGTTTTGTCTGCAATCAGCCTACGCTTGGCATTTTTAATGTGGCTCATGAGATACGAAAGAAGATGGCTGAGGATAAATAG

Protein sequence

>MS.gene28792.t1

MENSEAKSAENQPTEQVCSFFRKPVNRKNMRKRTIENEDNEDDSNNEESLMHVQKKNMKADNKLFFSTGSSKSSASAKLNEEPEKPSFHFESSKEIQVQHDSKATATLETETDFSRDARAIRERALKQATESLKGKSTGSEDVKLYKGINNYTDHKAGFRREQTIASEKAGGSHGPLRASAHIRVSARFDYQPDICKDYKETGYCGYGDSCKFMHDRGDYKSGWQMEKEWDEAEKARKMRLAAGEDAEEEGASLNDEDDDEDVLPFACFICRNPFVDPVSTKCKHYFCEHCALKHHAKNKKCFVCNQPTLGIFNVAHEIRKKMAEDK