Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29908.t1 | XP_013445572.1 | 98.2 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 5.70E-54 | 219.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29908.t1 | Q22799 | 55.1 | 89 | 38 | 1 | 1 | 89 | 1 | 87 | 4.6e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29908.t1 | A0A072TRW8 | 98.2 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 4.1e-54 | 219.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene29908 | MS.gene77822 | PPI |
| MS.gene29908 | MS.gene035363 | PPI |
| MS.gene035362 | MS.gene29908 | PPI |
| MS.gene29908 | MS.gene78481 | PPI |
| MS.gene29908 | MS.gene041599 | PPI |
| MS.gene29908 | MS.gene041598 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29908.t1 | MTR_8g464690 | 98.165 | 109 | 2 | 0 | 1 | 109 | 1 | 109 | 1.41e-76 | 221 |
| MS.gene29908.t1 | MTR_6g087770 | 51.087 | 92 | 45 | 0 | 1 | 92 | 1 | 92 | 4.05e-30 | 103 |
| MS.gene29908.t1 | MTR_2g101850 | 55.556 | 90 | 40 | 0 | 1 | 90 | 1 | 90 | 1.97e-29 | 101 |
| MS.gene29908.t1 | MTR_4g120150 | 48.837 | 86 | 42 | 1 | 5 | 90 | 46 | 129 | 7.92e-26 | 94.0 |
| MS.gene29908.t1 | MTR_4g114060 | 49.367 | 79 | 40 | 0 | 11 | 89 | 159 | 237 | 1.16e-21 | 85.9 |
| MS.gene29908.t1 | MTR_8g104900 | 45.882 | 85 | 44 | 1 | 12 | 94 | 45 | 129 | 4.08e-19 | 76.6 |
| MS.gene29908.t1 | MTR_1g031880 | 43.529 | 85 | 46 | 1 | 12 | 94 | 59 | 143 | 7.24e-19 | 76.6 |
| MS.gene29908.t1 | MTR_4g107350 | 50.633 | 79 | 38 | 1 | 12 | 89 | 222 | 300 | 3.36e-18 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29908.t1 | AT4G27360 | 70.874 | 103 | 30 | 0 | 1 | 103 | 1 | 103 | 1.27e-49 | 153 |
| MS.gene29908.t1 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 8.47e-33 | 110 |
| MS.gene29908.t1 | AT3G16120 | 55.435 | 92 | 41 | 0 | 1 | 92 | 1 | 92 | 8.47e-33 | 110 |
| MS.gene29908.t1 | AT1G52245 | 51.064 | 94 | 46 | 0 | 1 | 94 | 1 | 94 | 3.96e-30 | 103 |
| MS.gene29908.t1 | AT4G15930 | 49.412 | 85 | 41 | 1 | 5 | 89 | 39 | 121 | 7.17e-24 | 88.6 |
| MS.gene29908.t1 | AT5G20110 | 46.988 | 83 | 44 | 0 | 12 | 94 | 123 | 205 | 5.05e-21 | 83.6 |
| MS.gene29908.t1 | AT1G23220 | 39.773 | 88 | 52 | 1 | 8 | 94 | 38 | 125 | 5.50e-18 | 73.9 |
| MS.gene29908.t1 | AT4G15930 | 49.180 | 61 | 29 | 1 | 5 | 65 | 39 | 97 | 1.81e-13 | 61.2 |
Find 22 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTAGGCACTGATTT+TGG | 0.221609 | 8.3:+45529987 | MS.gene29908:CDS |
| CAGTTACATCATAGAAATCT+AGG | 0.300776 | 8.3:-45529806 | None:intergenic |
| AATTATTGCAGGAATTTGAT+AGG | 0.302502 | 8.3:+45529943 | MS.gene29908:intron |
| ATCATAGAAATCTAGGGCTT+TGG | 0.353606 | 8.3:-45529799 | None:intergenic |
| CTTCAATTCATCATGCTAGA+AGG | 0.354098 | 8.3:+45529711 | None:intergenic |
| TTGCTCTTTAAGGGATCTGC+TGG | 0.420352 | 8.3:+45530071 | MS.gene29908:CDS |
| CCTGGTTGGCAATGCATTGT+AGG | 0.440795 | 8.3:+45529975 | MS.gene29908:CDS |
| CTAGAAGGCAAGGCAGTGAT+TGG | 0.481296 | 8.3:+45529726 | MS.gene29908:CDS |
| CCTACAATGCATTGCCAACC+AGG | 0.504627 | 8.3:-45529975 | None:intergenic |
| AAATCAGTTCTCAGCAATAG+AGG | 0.512873 | 8.3:+45530109 | MS.gene29908:CDS |
| TATAGCTCGATTTATAAAGA+AGG | 0.514762 | 8.3:+45529839 | MS.gene29908:CDS |
| ATTATTGCAGGAATTTGATA+GGG | 0.521916 | 8.3:+45529944 | MS.gene29908:intron |
| CAGGAATTTGATAGGGTGCA+TGG | 0.526835 | 8.3:+45529951 | MS.gene29908:intron |
| TTTGATAGGGTGCATGGACC+TGG | 0.541477 | 8.3:+45529957 | MS.gene29908:CDS |
| TATAAATCGAGCTATATCAA+TGG | 0.561045 | 8.3:-45529832 | None:intergenic |
| ATCTGCTGGTCCACAAGCAG+AGG | 0.571115 | 8.3:+45530085 | MS.gene29908:CDS |
| ATTCATCATGCTAGAAGGCA+AGG | 0.581622 | 8.3:+45529716 | None:intergenic |
| TCCTTTGTGACACATTGTAG+TGG | 0.583602 | 8.3:+45530011 | MS.gene29908:CDS |
| AGTTACATCATAGAAATCTA+GGG | 0.585718 | 8.3:-45529805 | None:intergenic |
| ATAGGGTGCATGGACCTGGT+TGG | 0.652898 | 8.3:+45529961 | MS.gene29908:CDS |
| GCCACTACAATGTGTCACAA+AGG | 0.659746 | 8.3:-45530012 | None:intergenic |
| AGATTTCTATGATGTAACTG+AGG | 0.680263 | 8.3:+45529809 | MS.gene29908:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTATTGCAGGAATTTGAT+AGG | + | chr8.3:45529943-45529962 | MS.gene29908:intron | 25.0% |
| ! | AGTTACATCATAGAAATCTA+GGG | - | chr8.3:45529808-45529827 | None:intergenic | 25.0% |
| ! | ATGTGTTAGTTAATTATTGC+AGG | + | chr8.3:45529932-45529951 | MS.gene29908:intron | 25.0% |
| ! | ATTATTGCAGGAATTTGATA+GGG | + | chr8.3:45529944-45529963 | MS.gene29908:intron | 25.0% |
| ! | GGTAATAAAATAAAACCAAG+AGG | + | chr8.3:45529860-45529879 | MS.gene29908:intron | 25.0% |
| ! | TATAAATCGAGCTATATCAA+TGG | - | chr8.3:45529835-45529854 | None:intergenic | 25.0% |
| ! | TATAGCTCGATTTATAAAGA+AGG | + | chr8.3:45529839-45529858 | MS.gene29908:CDS | 25.0% |
| !!! | CTATTTTTGTATAGGTAGTA+TGG | + | chr8.3:45530043-45530062 | MS.gene29908:CDS | 25.0% |
| !!! | TGCTTCATCTATTTTTGTAT+AGG | + | chr8.3:45530035-45530054 | MS.gene29908:CDS | 25.0% |
| AAACCAAGAGGATTGTTTAT+TGG | + | chr8.3:45529872-45529891 | MS.gene29908:intron | 30.0% | |
| AGATTTCTATGATGTAACTG+AGG | + | chr8.3:45529809-45529828 | MS.gene29908:CDS | 30.0% | |
| CAGTTACATCATAGAAATCT+AGG | - | chr8.3:45529809-45529828 | None:intergenic | 30.0% | |
| TAACCAATAAACAATCCTCT+TGG | - | chr8.3:45529878-45529897 | None:intergenic | 30.0% | |
| ! | ATGGCAATTTTGCTCTTTAA+GGG | + | chr8.3:45530062-45530081 | MS.gene29908:CDS | 30.0% |
| ! | TATGGCAATTTTGCTCTTTA+AGG | + | chr8.3:45530061-45530080 | MS.gene29908:CDS | 30.0% |
| AAATCAGTTCTCAGCAATAG+AGG | + | chr8.3:45530109-45530128 | MS.gene29908:CDS | 35.0% | |
| ! | ATCATAGAAATCTAGGGCTT+TGG | - | chr8.3:45529802-45529821 | None:intergenic | 35.0% |
| TCCTTTGTGACACATTGTAG+TGG | + | chr8.3:45530011-45530030 | MS.gene29908:CDS | 40.0% | |
| !! | AACTGATTTTCCTCTGCTTG+TGG | - | chr8.3:45530098-45530117 | None:intergenic | 40.0% |
| !!! | TGCATTGTAGGCACTGATTT+TGG | + | chr8.3:45529987-45530006 | MS.gene29908:CDS | 40.0% |
| CAGGAATTTGATAGGGTGCA+TGG | + | chr8.3:45529951-45529970 | MS.gene29908:intron | 45.0% | |
| GCCACTACAATGTGTCACAA+AGG | - | chr8.3:45530015-45530034 | None:intergenic | 45.0% | |
| ! | TTGCTCTTTAAGGGATCTGC+TGG | + | chr8.3:45530071-45530090 | MS.gene29908:CDS | 45.0% |
| !!! | TTTTAATGTAATTTTTTTTA+TGG | + | chr8.3:45529898-45529917 | MS.gene29908:intron | 5.0% |
| CCTACAATGCATTGCCAACC+AGG | - | chr8.3:45529978-45529997 | None:intergenic | 50.0% | |
| CCTGGTTGGCAATGCATTGT+AGG | + | chr8.3:45529975-45529994 | MS.gene29908:CDS | 50.0% | |
| CTAGAAGGCAAGGCAGTGAT+TGG | + | chr8.3:45529726-45529745 | MS.gene29908:CDS | 50.0% | |
| TTTGATAGGGTGCATGGACC+TGG | + | chr8.3:45529957-45529976 | MS.gene29908:CDS | 50.0% | |
| ATAGGGTGCATGGACCTGGT+TGG | + | chr8.3:45529961-45529980 | MS.gene29908:CDS | 55.0% | |
| ATCTGCTGGTCCACAAGCAG+AGG | + | chr8.3:45530085-45530104 | MS.gene29908:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 45529723 | 45530145 | 45529723 | ID=MS.gene29908 |
| chr8.3 | mRNA | 45529723 | 45530145 | 45529723 | ID=MS.gene29908.t1;Parent=MS.gene29908 |
| chr8.3 | exon | 45529723 | 45529860 | 45529723 | ID=MS.gene29908.t1.exon1;Parent=MS.gene29908.t1 |
| chr8.3 | CDS | 45529723 | 45529860 | 45529723 | ID=cds.MS.gene29908.t1;Parent=MS.gene29908.t1 |
| chr8.3 | exon | 45529954 | 45530145 | 45529954 | ID=MS.gene29908.t1.exon2;Parent=MS.gene29908.t1 |
| chr8.3 | CDS | 45529954 | 45530145 | 45529954 | ID=cds.MS.gene29908.t1;Parent=MS.gene29908.t1 |
| Gene Sequence |
| Protein sequence |
