Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30335.t1 | XP_024629570.1 | 93.8 | 81 | 5 | 0 | 5 | 85 | 14 | 94 | 2.70E-19 | 104.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30335.t1 | A0A072TLP7 | 91.6 | 83 | 7 | 0 | 3 | 85 | 11 | 93 | 3.3e-19 | 103.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050721 | MS.gene30335 | 0.80409 | 2.55E-49 | -1.69E-46 |
| MS.gene050815 | MS.gene30335 | 0.82437 | 8.37E-54 | -1.69E-46 |
| MS.gene053008 | MS.gene30335 | 0.815 | 1.16E-51 | -1.69E-46 |
| MS.gene057140 | MS.gene30335 | 0.818225 | 2.19E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene30335 | 0.832497 | 9.14E-56 | -1.69E-46 |
| MS.gene059151 | MS.gene30335 | 0.807738 | 4.37E-50 | -1.69E-46 |
| MS.gene059898 | MS.gene30335 | 0.811494 | 6.81E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene30335 | 0.839795 | 1.28E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30335.t1 | MTR_8g017610 | 97.561 | 82 | 2 | 0 | 4 | 85 | 15 | 96 | 3.09e-51 | 155 |
| MS.gene30335.t1 | MTR_8g017610 | 97.561 | 82 | 2 | 0 | 4 | 85 | 12 | 93 | 3.40e-51 | 155 |
| MS.gene30335.t1 | MTR_2g039890 | 41.406 | 128 | 18 | 3 | 5 | 75 | 52 | 179 | 3.77e-17 | 72.0 |
| MS.gene30335.t1 | MTR_2g039890 | 51.899 | 79 | 37 | 1 | 4 | 81 | 5 | 83 | 2.32e-16 | 69.7 |
| MS.gene30335.t1 | MTR_2g039890 | 41.406 | 128 | 18 | 3 | 5 | 75 | 99 | 226 | 4.65e-17 | 72.4 |
| MS.gene30335.t1 | MTR_2g039890 | 56.522 | 69 | 29 | 1 | 4 | 71 | 52 | 120 | 2.84e-16 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30335.t1 | AT1G72690 | 57.895 | 76 | 30 | 1 | 4 | 77 | 10 | 85 | 1.49e-25 | 90.9 |
| MS.gene30335.t1 | AT5G57000 | 54.795 | 73 | 29 | 1 | 7 | 75 | 23 | 95 | 2.74e-18 | 73.9 |
| MS.gene30335.t1 | AT5G57000 | 54.795 | 73 | 29 | 1 | 7 | 75 | 70 | 142 | 6.97e-18 | 73.9 |
| MS.gene30335.t1 | AT1G17490 | 50.725 | 69 | 27 | 1 | 10 | 78 | 15 | 76 | 3.92e-17 | 69.3 |
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGTGTCAGATACTTTCTT+GGG | 0.308999 | 8.1:+80057611 | None:intergenic |
| CTTAGTGTCAGATACTTTCT+TGG | 0.343708 | 8.1:+80057610 | None:intergenic |
| TTAGCCCTCTCAAGAATGTT+TGG | 0.369322 | 8.1:+80057803 | None:intergenic |
| AACAGCTATGATCACTTTGA+TGG | 0.413880 | 8.1:+80057581 | None:intergenic |
| TTCCATGAGTTTCTTTGTCA+TGG | 0.419220 | 8.1:+80057738 | None:intergenic |
| AGATACTTTCTTGGGGAGAA+TGG | 0.422648 | 8.1:+80057619 | None:intergenic |
| AAGCTCCCGGTGTGTTTGAA+AGG | 0.468558 | 8.1:-80057673 | MS.gene30335:CDS |
| AGCTCCCGGTGTGTTTGAAA+GGG | 0.525240 | 8.1:-80057672 | MS.gene30335:CDS |
| CGCCATGACAAAGAAACTCA+TGG | 0.536708 | 8.1:-80057740 | MS.gene30335:CDS |
| CAAGAAAGTATCTGACACTA+AGG | 0.540357 | 8.1:-80057609 | MS.gene30335:CDS |
| CATTCTTGAGAGGGCTAAGG+AGG | 0.553782 | 8.1:-80057798 | MS.gene30335:CDS |
| AAACATTCTTGAGAGGGCTA+AGG | 0.562461 | 8.1:-80057801 | MS.gene30335:CDS |
| CTTCACCCTTTCAAACACAC+CGG | 0.571153 | 8.1:+80057667 | None:intergenic |
| AGCTCCAAACATTCTTGAGA+GGG | 0.578483 | 8.1:-80057807 | MS.gene30335:CDS |
| GTTGAAGAAGTCAAAGCTCC+CGG | 0.580117 | 8.1:-80057686 | MS.gene30335:CDS |
| TGAGTTTCTTTGTCATGGCG+AGG | 0.584602 | 8.1:+80057743 | None:intergenic |
| CGGTGTGTTTGAAAGGGTGA+AGG | 0.593554 | 8.1:-80057666 | MS.gene30335:CDS |
| TTCACCCTTTCAAACACACC+GGG | 0.602200 | 8.1:+80057668 | None:intergenic |
| AAGCTCCAAACATTCTTGAG+AGG | 0.602370 | 8.1:-80057808 | MS.gene30335:CDS |
| TAGTGTCAGATACTTTCTTG+GGG | 0.605693 | 8.1:+80057612 | None:intergenic |
| GAGGGCTAAGGAGGAGACTG+AGG | 0.618637 | 8.1:-80057789 | MS.gene30335:CDS |
| AGAGCTTGAAGCTGTAGTTG+AGG | 0.634155 | 8.1:-80057642 | MS.gene30335:CDS |
| ATGACAAAGAAACTCATGGA+AGG | 0.643744 | 8.1:-80057736 | MS.gene30335:CDS |
| AAGGAATGATGACATTGATG+AGG | 0.657096 | 8.1:-80057717 | MS.gene30335:CDS |
| AGGAATGATGACATTGATGA+GGG | 0.670735 | 8.1:-80057716 | MS.gene30335:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGACTATTTTATAAATGATT+TGG | - | chr8.1:80057668-80057687 | MS.gene30335:CDS | 15.0% |
| !! | ATGAAACAGAATATACAAAA+AGG | + | chr8.1:80057715-80057734 | None:intergenic | 20.0% |
| !! | TGAAACAGAATATACAAAAA+GGG | + | chr8.1:80057714-80057733 | None:intergenic | 20.0% |
| !!! | GATAAGTAATTATTGGTTTT+AGG | - | chr8.1:80057594-80057613 | MS.gene30335:CDS | 20.0% |
| !!! | TATTGGTTTTAGGTATATGT+GGG | - | chr8.1:80057604-80057623 | MS.gene30335:CDS | 25.0% |
| !!! | TTATTGGTTTTAGGTATATG+TGG | - | chr8.1:80057603-80057622 | MS.gene30335:CDS | 25.0% |
| TTAGTGTCAGATACTTTCTT+GGG | + | chr8.1:80057954-80057973 | None:intergenic | 30.0% | |
| AAGGAATGATGACATTGATG+AGG | - | chr8.1:80057845-80057864 | MS.gene30335:intron | 35.0% | |
| AGGAATGATGACATTGATGA+GGG | - | chr8.1:80057846-80057865 | MS.gene30335:intron | 35.0% | |
| ATGACAAAGAAACTCATGGA+AGG | - | chr8.1:80057826-80057845 | MS.gene30335:intron | 35.0% | |
| CTTAGTGTCAGATACTTTCT+TGG | + | chr8.1:80057955-80057974 | None:intergenic | 35.0% | |
| TAGTGTCAGATACTTTCTTG+GGG | + | chr8.1:80057953-80057972 | None:intergenic | 35.0% | |
| TTCCATGAGTTTCTTTGTCA+TGG | + | chr8.1:80057827-80057846 | None:intergenic | 35.0% | |
| !! | CAAGAAAGTATCTGACACTA+AGG | - | chr8.1:80057953-80057972 | MS.gene30335:intron | 35.0% |
| AAACATTCTTGAGAGGGCTA+AGG | - | chr8.1:80057761-80057780 | MS.gene30335:CDS | 40.0% | |
| AAGCTCCAAACATTCTTGAG+AGG | - | chr8.1:80057754-80057773 | MS.gene30335:CDS | 40.0% | |
| AGATACTTTCTTGGGGAGAA+TGG | + | chr8.1:80057946-80057965 | None:intergenic | 40.0% | |
| AGCTCCAAACATTCTTGAGA+GGG | - | chr8.1:80057755-80057774 | MS.gene30335:CDS | 40.0% | |
| TTAGCCCTCTCAAGAATGTT+TGG | + | chr8.1:80057762-80057781 | None:intergenic | 40.0% | |
| AGAGCTTGAAGCTGTAGTTG+AGG | - | chr8.1:80057920-80057939 | MS.gene30335:intron | 45.0% | |
| CGCCATGACAAAGAAACTCA+TGG | - | chr8.1:80057822-80057841 | MS.gene30335:CDS | 45.0% | |
| CTTCACCCTTTCAAACACAC+CGG | + | chr8.1:80057898-80057917 | None:intergenic | 45.0% | |
| GTTGAAGAAGTCAAAGCTCC+CGG | - | chr8.1:80057876-80057895 | MS.gene30335:intron | 45.0% | |
| TGAGTTTCTTTGTCATGGCG+AGG | + | chr8.1:80057822-80057841 | None:intergenic | 45.0% | |
| TTCACCCTTTCAAACACACC+GGG | + | chr8.1:80057897-80057916 | None:intergenic | 45.0% | |
| AAGCTCCCGGTGTGTTTGAA+AGG | - | chr8.1:80057889-80057908 | MS.gene30335:intron | 50.0% | |
| AGCTCCCGGTGTGTTTGAAA+GGG | - | chr8.1:80057890-80057909 | MS.gene30335:intron | 50.0% | |
| CATTCTTGAGAGGGCTAAGG+AGG | - | chr8.1:80057764-80057783 | MS.gene30335:CDS | 50.0% | |
| CGGTGTGTTTGAAAGGGTGA+AGG | - | chr8.1:80057896-80057915 | MS.gene30335:intron | 50.0% | |
| GAGGGCTAAGGAGGAGACTG+AGG | - | chr8.1:80057773-80057792 | MS.gene30335:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 80057592 | 80057992 | 80057592 | ID=MS.gene30335 |
| chr8.1 | mRNA | 80057592 | 80057992 | 80057592 | ID=MS.gene30335.t1;Parent=MS.gene30335 |
| chr8.1 | exon | 80057986 | 80057992 | 80057986 | ID=MS.gene30335.t1.exon1;Parent=MS.gene30335.t1 |
| chr8.1 | CDS | 80057986 | 80057992 | 80057986 | ID=cds.MS.gene30335.t1;Parent=MS.gene30335.t1 |
| chr8.1 | exon | 80057592 | 80057842 | 80057592 | ID=MS.gene30335.t1.exon2;Parent=MS.gene30335.t1 |
| chr8.1 | CDS | 80057592 | 80057842 | 80057592 | ID=cds.MS.gene30335.t1;Parent=MS.gene30335.t1 |
| Gene Sequence |
| Protein sequence |
