Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30828.t1 | PNX87361.1 | 62.7 | 177 | 21 | 2 | 21 | 154 | 57 | 231 | 6.60E-46 | 195.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30828.t1 | P0C2F6 | 28.2 | 255 | 103 | 10 | 293 | 475 | 365 | 611 | 3.6e-08 | 61.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30828.t1 | A0A2K3M992 | 62.7 | 177 | 21 | 2 | 21 | 154 | 57 | 231 | 4.7e-46 | 195.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049585 | MS.gene30828 | 0.813521 | 2.45E-51 | -1.69E-46 |
| MS.gene051124 | MS.gene30828 | 0.809495 | 1.84E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene30828 | 0.810688 | 1.02E-50 | -1.69E-46 |
| MS.gene051800 | MS.gene30828 | 0.812307 | 4.53E-51 | -1.69E-46 |
| MS.gene05191 | MS.gene30828 | 0.805087 | 1.58E-49 | -1.69E-46 |
| MS.gene052168 | MS.gene30828 | 0.828117 | 1.07E-54 | -1.69E-46 |
| MS.gene052447 | MS.gene30828 | 0.806255 | 8.99E-50 | -1.69E-46 |
| MS.gene056162 | MS.gene30828 | 0.819971 | 8.77E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene30828 | 0.805646 | 1.21E-49 | -1.69E-46 |
| MS.gene056973 | MS.gene30828 | 0.801687 | 8.01E-49 | -1.69E-46 |
| MS.gene057598 | MS.gene30828 | 0.828514 | 8.62E-55 | -1.69E-46 |
| MS.gene057600 | MS.gene30828 | 0.810273 | 1.25E-50 | -1.69E-46 |
| MS.gene058919 | MS.gene30828 | 0.811177 | 7.98E-51 | -1.69E-46 |
| MS.gene05897 | MS.gene30828 | 0.804789 | 1.83E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene30828 | 0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene060134 | MS.gene30828 | 0.821394 | 4.13E-53 | -1.69E-46 |
| MS.gene060135 | MS.gene30828 | 0.809618 | 1.73E-50 | -1.69E-46 |
| MS.gene060918 | MS.gene30828 | 0.817943 | 2.54E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 107 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAATTTCACCCAATTTAA+AGG | 0.164462 | 6.4:+38069199 | None:intergenic |
| GGGTCTGCCCACCTTTAAAT+TGG | 0.190230 | 6.4:-38069210 | MS.gene30828:CDS |
| CAGCATGGTGCCAACACTTT+TGG | 0.219832 | 6.4:-38076372 | MS.gene30828:CDS |
| TCTCTCGTCATGTTGTCTTT+TGG | 0.221234 | 6.4:-38074981 | MS.gene30828:CDS |
| GATTCCATGACCTCAGTATT+TGG | 0.236015 | 6.4:+38069244 | None:intergenic |
| ACCAGTTGAGATTGTACTTT+TGG | 0.253066 | 6.4:+38069649 | None:intergenic |
| TGGCAGATGCTTTAGCTAAA+TGG | 0.257488 | 6.4:-38068838 | MS.gene30828:CDS |
| CTTATGCAGCTTCTTCCTTC+TGG | 0.263453 | 6.4:-38076264 | MS.gene30828:CDS |
| CTAGTGTCTCAAGGTTATTT+AGG | 0.278289 | 6.4:-38075755 | MS.gene30828:CDS |
| GAGACATATGCAGGTTCTTC+TGG | 0.279712 | 6.4:-38074844 | MS.gene30828:CDS |
| ATGCGAATGCGCTGGGATAT+TGG | 0.329887 | 6.4:+38075003 | None:intergenic |
| TCATGGAATCCGAGATTTCT+TGG | 0.333676 | 6.4:-38069231 | MS.gene30828:CDS |
| AATTAGTGAGTTAGTGAATC+AGG | 0.345071 | 6.4:-38069326 | MS.gene30828:CDS |
| TCAACTTTGGCATCGTCGTC+TGG | 0.355533 | 6.4:-38075807 | MS.gene30828:CDS |
| GGCAGATGCTTTAGCTAAAT+GGG | 0.359621 | 6.4:-38068837 | MS.gene30828:CDS |
| CAAATTCTTCCATTCAACTT+TGG | 0.364258 | 6.4:-38075820 | MS.gene30828:CDS |
| GTGTTAAATACTTCTTTGAT+TGG | 0.390194 | 6.4:+38069605 | None:intergenic |
| GGTCTGCCCACCTTTAAATT+GGG | 0.390565 | 6.4:-38069209 | MS.gene30828:CDS |
| TAAACTCTCCGATAGAATTT+CGG | 0.395964 | 6.4:+38069120 | None:intergenic |
| AGCTCAACGGTGGGAGGTGC+AGG | 0.400522 | 6.4:+38074760 | None:intergenic |
| CCGCGAACAGGACAACTCAT+AGG | 0.424850 | 6.4:-38075929 | MS.gene30828:CDS |
| TGGTGACATTAGATTGTGTT+TGG | 0.430303 | 6.4:-38069390 | MS.gene30828:CDS |
| CATGGAATCCGAGATTTCTT+GGG | 0.438544 | 6.4:-38069230 | MS.gene30828:CDS |
| AACACAAAGGTTATCGATGT+TGG | 0.442481 | 6.4:-38075029 | MS.gene30828:intron |
| TAGATTGTGTTTGGAAAGCT+AGG | 0.445799 | 6.4:-38069381 | MS.gene30828:CDS |
| AGAAGATCCTAGAGATTCAT+TGG | 0.455887 | 6.4:+38076323 | None:intergenic |
| GATCGACTTAAACTTTGATA+AGG | 0.459913 | 6.4:+38074939 | None:intergenic |
| GACGAGAGATGCGAATGCGC+TGG | 0.462427 | 6.4:+38074995 | None:intergenic |
| GAAACTTTGGCAGGAGCTGT+TGG | 0.468092 | 6.4:-38069509 | MS.gene30828:intron |
| TCCAAAAGTACAATCTCAAC+TGG | 0.468811 | 6.4:-38069650 | MS.gene30828:CDS |
| AATGGAAGAATTTGAATTTG+TGG | 0.469175 | 6.4:+38075829 | None:intergenic |
| GCACAAATGTTGGACTCAAT+AGG | 0.477159 | 6.4:+38075857 | None:intergenic |
| AAGAGAGGAGTGGAAGTAGA+CGG | 0.478939 | 6.4:+38074916 | None:intergenic |
| CCTATGAGTTGTCCTGTTCG+CGG | 0.480997 | 6.4:+38075929 | None:intergenic |
| TCGTGTGCAGATCCGCGAAC+AGG | 0.481779 | 6.4:-38075941 | MS.gene30828:intron |
| AGAATTTGAATTTGTGGTAG+TGG | 0.484405 | 6.4:+38075835 | None:intergenic |
| TGCTTAATTTCAGGCAGCAA+AGG | 0.487320 | 6.4:-38069699 | MS.gene30828:intron |
| TCCTCCAAATACTGAGGTCA+TGG | 0.492512 | 6.4:-38069248 | MS.gene30828:CDS |
| ACGAGAGATGCGAATGCGCT+GGG | 0.492838 | 6.4:+38074996 | None:intergenic |
| ACACAAAGGTTATCGATGTT+GGG | 0.496437 | 6.4:-38075028 | MS.gene30828:intron |
| ATGTCGCAGAACTCATGAAT+TGG | 0.500945 | 6.4:-38069302 | MS.gene30828:CDS |
| ACGACGATGCCAAAGTTGAA+TGG | 0.516259 | 6.4:+38075811 | None:intergenic |
| AGAGAGGAGTGGAAGTAGAC+GGG | 0.518922 | 6.4:+38074917 | None:intergenic |
| CCATTTAGAATTACCTGGTG+TGG | 0.519187 | 6.4:+38076218 | None:intergenic |
| ATAGGGAGTGGGCGTAAGAT+CGG | 0.528456 | 6.4:-38075911 | MS.gene30828:CDS |
| GGGTTAAGTGCAAGTTATGC+CGG | 0.529646 | 6.4:-38069151 | MS.gene30828:CDS |
| AAACTTTGGCAGGAGCTGTT+GGG | 0.532809 | 6.4:-38069508 | MS.gene30828:intron |
| GATTGAACATCGGAAGGGGT+TGG | 0.534132 | 6.4:+38074814 | None:intergenic |
| TTTCCTAGTGACACACATGC+AGG | 0.536283 | 6.4:-38074871 | MS.gene30828:CDS |
| CTTTGATTGGCACTCGAGGA+AGG | 0.538329 | 6.4:+38069618 | None:intergenic |
| ATTTAGATTGATGTCAGTCA+TGG | 0.542931 | 6.4:-38068903 | MS.gene30828:intron |
| TCCACTCAACAATGTTACCT+TGG | 0.543032 | 6.4:+38069461 | None:intergenic |
| CGCGAACAGGACAACTCATA+GGG | 0.545446 | 6.4:-38075928 | MS.gene30828:CDS |
| AATATTCATGACAGAGGAGG+TGG | 0.550351 | 6.4:+38076296 | None:intergenic |
| CGTCTGGCTCATAGTTCTAT+CGG | 0.550611 | 6.4:-38075791 | MS.gene30828:CDS |
| TGTGGTAGTGGCACAAATGT+TGG | 0.551295 | 6.4:+38075847 | None:intergenic |
| GCAGGTTCTGAGACATATGC+AGG | 0.555326 | 6.4:-38074853 | MS.gene30828:CDS |
| GAAGTGGATTGAACATCGGA+AGG | 0.556207 | 6.4:+38074808 | None:intergenic |
| AATTTCACCCAATTTAAAGG+TGG | 0.556608 | 6.4:+38069202 | None:intergenic |
| GTTGCTGCCAATGAATCTCT+AGG | 0.564740 | 6.4:-38076330 | MS.gene30828:CDS |
| TGGGCAGACCCAAGAAATCT+CGG | 0.568421 | 6.4:+38069222 | None:intergenic |
| GAACCTGCATGTGTGTCACT+AGG | 0.573102 | 6.4:+38074868 | None:intergenic |
| TGTGATGGTGCTGTGAATCA+AGG | 0.575441 | 6.4:-38069175 | MS.gene30828:CDS |
| CTACAGAGAAGCAAATAAAG+TGG | 0.578768 | 6.4:-38068858 | MS.gene30828:CDS |
| ATTTCACCCAATTTAAAGGT+GGG | 0.579185 | 6.4:+38069203 | None:intergenic |
| AGTGGATTGAACATCGGAAG+GGG | 0.579979 | 6.4:+38074810 | None:intergenic |
| CTCGTAGGAAGCTCAACGGT+GGG | 0.583339 | 6.4:+38074751 | None:intergenic |
| ACAGGACAACTCATAGGGAG+TGG | 0.585218 | 6.4:-38075923 | MS.gene30828:CDS |
| TAGAGATTCATTGGCAGCAA+CGG | 0.585616 | 6.4:+38076332 | None:intergenic |
| ATCGCTCTTTCAACCACACC+AGG | 0.587970 | 6.4:-38076231 | MS.gene30828:intron |
| TTGCGTCCTCTAGTGTCTCA+AGG | 0.591211 | 6.4:-38075764 | MS.gene30828:CDS |
| TCCAAGGTAACATTGTTGAG+TGG | 0.592664 | 6.4:-38069462 | MS.gene30828:CDS |
| GGTGGGAGGTGCAGGATCAA+CGG | 0.594501 | 6.4:+38074768 | None:intergenic |
| TGGGTGAAATTGAATTGTGA+TGG | 0.597428 | 6.4:-38069190 | MS.gene30828:CDS |
| GCAGATGCTTTAGCTAAATG+GGG | 0.609226 | 6.4:-38068836 | MS.gene30828:CDS |
| ACTTCTTTGATTGGCACTCG+AGG | 0.609949 | 6.4:+38069614 | None:intergenic |
| CACCGTTGAGCTTCCTACGA+GGG | 0.613544 | 6.4:-38074749 | MS.gene30828:intron |
| GGAGTGGGCGTAAGATCGGA+AGG | 0.615494 | 6.4:-38075907 | MS.gene30828:CDS |
| GGAGGTGCAGGATCAACGGT+CGG | 0.615563 | 6.4:+38074772 | None:intergenic |
| GCGATAGGAGTATTACCAGA+AGG | 0.616812 | 6.4:+38076249 | None:intergenic |
| AGTGTTATCCGAAATTCTAT+CGG | 0.616967 | 6.4:-38069128 | MS.gene30828:CDS |
| TCCATGACCTCAGTATTTGG+AGG | 0.620401 | 6.4:+38069247 | None:intergenic |
| TCGCAGAACTCATGAATTGG+TGG | 0.625167 | 6.4:-38069299 | MS.gene30828:CDS |
| CAGGACAACTCATAGGGAGT+GGG | 0.626490 | 6.4:-38075922 | MS.gene30828:CDS |
| TAGAATTTCGGATAACACTC+CGG | 0.626837 | 6.4:+38069132 | None:intergenic |
| GGTGGATTTCTTACCCTCGT+AGG | 0.627964 | 6.4:+38074736 | None:intergenic |
| ACAACATCAAAATAGTGAGT+CGG | 0.630081 | 6.4:+38068797 | None:intergenic |
| ATCAGAAGTGGATTGAACAT+CGG | 0.632450 | 6.4:+38074804 | None:intergenic |
| AAGTGGATTGAACATCGGAA+GGG | 0.632455 | 6.4:+38074809 | None:intergenic |
| GGTGTGGTTGAAAGAGCGAT+AGG | 0.632828 | 6.4:+38076234 | None:intergenic |
| CTTAATATTCATGACAGAGG+AGG | 0.636535 | 6.4:+38076293 | None:intergenic |
| ACATCAGGGGTAGTAAAGAG+AGG | 0.636790 | 6.4:+38074901 | None:intergenic |
| TAGTACAGACCCAAAAGTGT+TGG | 0.638249 | 6.4:+38076362 | None:intergenic |
| CCACCGTTGAGCTTCCTACG+AGG | 0.643687 | 6.4:-38074750 | MS.gene30828:CDS |
| TGAGTGGAGAATCAAATTTG+TGG | 0.646612 | 6.4:-38069410 | MS.gene30828:CDS |
| AGGGGTAGTAAAGAGAGGAG+TGG | 0.648306 | 6.4:+38074906 | None:intergenic |
| TCTCATGTTTGTCATCAGCA+TGG | 0.649550 | 6.4:-38076387 | MS.gene30828:CDS |
| CAGATGCTTTAGCTAAATGG+GGG | 0.649735 | 6.4:-38068835 | MS.gene30828:CDS |
| AGACTTAATATTCATGACAG+AGG | 0.650554 | 6.4:+38076290 | None:intergenic |
| CCTCGTAGGAAGCTCAACGG+TGG | 0.653933 | 6.4:+38074750 | None:intergenic |
| GATGGTGCTGTGAATCAAGG+TGG | 0.664465 | 6.4:-38069172 | MS.gene30828:CDS |
| AAATAACCTTGAGACACTAG+AGG | 0.666797 | 6.4:+38075758 | None:intergenic |
| GTAGGAAGCTCAACGGTGGG+AGG | 0.669516 | 6.4:+38074754 | None:intergenic |
| TACCCTCGTAGGAAGCTCAA+CGG | 0.677089 | 6.4:+38074747 | None:intergenic |
| TGCTTGATAAATCTTGTGAG+TGG | 0.677614 | 6.4:-38069426 | MS.gene30828:CDS |
| TCATTCTCCTCCAAATACTG+AGG | 0.678592 | 6.4:-38069254 | MS.gene30828:CDS |
| ATGGTGCTGTGAATCAAGGT+GGG | 0.690520 | 6.4:-38069171 | MS.gene30828:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 38068739 | 38076418 | 38068739 | ID=MS.gene30828 |
| chr6.4 | mRNA | 38068739 | 38076418 | 38068739 | ID=MS.gene30828.t1;Parent=MS.gene30828 |
| chr6.4 | exon | 38076232 | 38076418 | 38076232 | ID=MS.gene30828.t1.exon1;Parent=MS.gene30828.t1 |
| chr6.4 | CDS | 38076232 | 38076418 | 38076232 | ID=cds.MS.gene30828.t1;Parent=MS.gene30828.t1 |
| chr6.4 | exon | 38075681 | 38075953 | 38075681 | ID=MS.gene30828.t1.exon2;Parent=MS.gene30828.t1 |
| chr6.4 | CDS | 38075681 | 38075953 | 38075681 | ID=cds.MS.gene30828.t1;Parent=MS.gene30828.t1 |
| chr6.4 | exon | 38074750 | 38075042 | 38074750 | ID=MS.gene30828.t1.exon3;Parent=MS.gene30828.t1 |
| chr6.4 | CDS | 38074750 | 38075042 | 38074750 | ID=cds.MS.gene30828.t1;Parent=MS.gene30828.t1 |
| chr6.4 | exon | 38069616 | 38069708 | 38069616 | ID=MS.gene30828.t1.exon4;Parent=MS.gene30828.t1 |
| chr6.4 | CDS | 38069616 | 38069708 | 38069616 | ID=cds.MS.gene30828.t1;Parent=MS.gene30828.t1 |
| chr6.4 | exon | 38069106 | 38069518 | 38069106 | ID=MS.gene30828.t1.exon5;Parent=MS.gene30828.t1 |
| chr6.4 | CDS | 38069106 | 38069518 | 38069106 | ID=cds.MS.gene30828.t1;Parent=MS.gene30828.t1 |
| chr6.4 | exon | 38068739 | 38068919 | 38068739 | ID=MS.gene30828.t1.exon6;Parent=MS.gene30828.t1 |
| chr6.4 | CDS | 38068739 | 38068919 | 38068739 | ID=cds.MS.gene30828.t1;Parent=MS.gene30828.t1 |
| Gene Sequence |
| Protein sequence |
