AlfalfaGEDB | MS.gene060135

M. sativa cultivar XinJiangDaYe / MS.gene060135

KEGG: K04773
GO: GO:0003674 GO:0003824 GO:0005575 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009987 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0034641 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:0110165 GO:0140096 GO:1901564
PFAM: PF01343.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060135.t1	PNY04437.1	47.1	573	258	10	46	587	23	581	1.90E-127	466.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060135.t1	Q9C9C0	43.1	531	266	8	80	587	90	607	6.3e-108	392.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060135.t1	A0A2K3NN31	47.1	573	258	10	46	587	23	581	1.4e-127	466.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052447	MS.gene060135	0.818726	1.69E-52	-1.69E-46
MS.gene060134	MS.gene060135	0.979032	4.36E-147	-1.69E-46
MS.gene060135	MS.gene060332	0.893386	6.60E-75	-1.69E-46
MS.gene060135	MS.gene060334	0.804406	2.19E-49	-1.69E-46
MS.gene060135	MS.gene060373	0.802041	6.77E-49	-1.69E-46
MS.gene060135	MS.gene069743	0.816053	6.74E-52	-1.69E-46
MS.gene060135	MS.gene24518	0.830798	2.40E-55	-1.69E-46
MS.gene060135	MS.gene30828	0.809618	1.73E-50	-1.69E-46
MS.gene060135	MS.gene49642	0.807987	3.86E-50	-1.69E-46
MS.gene060135	MS.gene50865	0.805055	1.61E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060135.t1	MTR_4g112860	45.392	586	271	11	36	587	19	589	1.18e-153	456
MS.gene060135.t1	MTR_2g058970	44.444	531	259	7	80	587	84	601	8.74e-146	436
MS.gene060135.t1	MTR_4g112860	42.857	532	255	11	36	533	19	535	4.22e-123	375
MS.gene060135.t1	MTR_4g112820	38.241	557	231	12	56	586	16	485	5.56e-104	323
MS.gene060135.t1	MTR_4g112860	41.365	498	237	12	36	499	19	495	1.02e-101	317
MS.gene060135.t1	MTR_4g123500	41.379	464	237	8	131	587	5	440	7.49e-98	306
MS.gene060135.t1	MTR_4g112860	38.987	395	192	11	36	396	19	398	3.28e-68	226
MS.gene060135.t1	MTR_5g025810	32.781	543	206	15	56	587	11	405	4.23e-67	228
MS.gene060135.t1	MTR_4g130360	35.054	465	203	15	4	451	6	388	3.39e-65	221
MS.gene060135.t1	MTR_4g130360	62.712	59	20	1	532	588	388	446	5.50e-14	75.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060135.t1	AT1G73990	43.126	531	266	8	80	587	90	607	5.92e-133	404
MS.gene060135.t1	AT1G73990	40.461	477	248	8	80	533	90	553	2.30e-105	330

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Find 118 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGGTCCACAATCGAAGA+TGG	0.562971	8.4:-4082932	None:intergenic
AAAGTGTGAATGGTTTGCGA+TGG	0.637871	8.4:-4082843	None:intergenic
AACAGTGTTGATTATTCGAC+CGG	0.483950	8.4:+4083025	MS.gene060135:CDS
AACCTCTACGATCTCTTCTC+CGG	0.527699	8.4:+4083462	MS.gene060135:CDS
AACTATTTGAGAATGATCAT+CGG	0.625097	8.4:+4084274	MS.gene060135:CDS
AAGAAAATATATGAATATTT+TGG	0.176804	8.4:+4084213	MS.gene060135:CDS
AAGAAAGAGGTTCTTTCTAA+AGG	0.390182	8.4:+4084240	MS.gene060135:CDS
AAGATCTAATTGGCTTGATA+TGG	0.258838	8.4:+4083639	MS.gene060135:CDS
AATAGGTTGGTTGATGCGCT+AGG	0.587278	8.4:+4084453	MS.gene060135:CDS
AATGTGAATGGTCTGTGAAT+GGG	0.582701	8.4:-4082968	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAAGAGGTTCTTTCTAA+AGG	+	chr8.4:4084240-4084259	MS.gene060135:CDS	30.0%
AGGCTAATATACCTCAAAAT+AGG	+	chr8.4:4084511-4084530	MS.gene060135:CDS	30.0%
ATCGGTATTATTAGAGTTTG+TGG	+	chr8.4:4083886-4083905	MS.gene060135:CDS	30.0%
ATTACACCAATCTTATCGAA+TGG	+	chr8.4:4083530-4083549	MS.gene060135:CDS	30.0%
ATTTGATTGAGAATATCCGT+AGG	+	chr8.4:4083944-4083963	MS.gene060135:CDS	30.0%
CTGTTGGTATATCAAATAGT+AGG	+	chr8.4:4083857-4083876	MS.gene060135:CDS	30.0%
CTTAAATTCAGATGAAGGAT+GGG	+	chr8.4:4083588-4083607	MS.gene060135:CDS	30.0%
GATGAAAAGTAGAATGTGAA+TGG	-	chr8.4:4082983-4083002	None:intergenic	30.0%
GATGAGAAATAAAGTGTGAA+TGG	-	chr8.4:4082856-4082875	None:intergenic	30.0%
GATGGTGACAAGATTATTAT+GGG	+	chr8.4:4083772-4083791	MS.gene060135:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	4082797	4084563	4082797	ID=MS.gene060135
chr8.4	mRNA	4082797	4084563	4082797	ID=MS.gene060135.t1;Parent=MS.gene060135
chr8.4	exon	4082797	4084563	4082797	ID=MS.gene060135.t1.exon1;Parent=MS.gene060135.t1
chr8.4	CDS	4082797	4084563	4082797	ID=cds.MS.gene060135.t1;Parent=MS.gene060135.t1

Gene Sequence

>MS.gene060135

ATGACAATCCTCCGTAACATGTACAGAAAAATATCACGATCATCCTCCATCGCAAACCATTCACACTTTATTTCTCATCCTCTTCATCTTACGGAAACCCCTCAAATTCACAAACCCCTCAAATTCCACTGCACACCATCTTCGATTGTGGACCGTTTTAAATCTTTTCGACCCATTCACAGACCATTCACATTCTACTTTTCATCCACTTCTACTCCAGGTAAAGGTAACAGTGTTGATTATTCGACCGGAGATTTCGATTTCGTTCCGCTCTCTGGTTTTAAGAAATATATGGTGAAGATCAAGACTCTGTTAGCCTCGGAGCGTGTCGAACATGGTAGTGTTTTGAGAATCAATTTGGAAGGAAAGTTTTCAGATGGACCAGTTGGCACCGACTCTCAGGTATTGTCTTTACCTCAACTAAGCGAAAATCTTATGAAAGCCGCATATGATCCTCGCGTGTCTGCTCTATTTGTTTATATAGATGCTTTTGATTGTGGTTGGGGCAAAGCCGATGAAATTAGAAGACAGATATTCAATTTCAAGAAATCTGGTAAAAAAGTTATTGCTTTTATGCCTACATTTTGGGGTTTTGAGAGTGGAGTTTGGTATCAAAATAAATCATATTACATAGCTAGTGAATGTCATGAAATCTATGCACCTCCAACCTCTACGATCTCTTCTCCGGAGACAACTACTCTTACTAAGATTACTAAGAGATCTTTGAAAAAGGATTACACCAATCTTATCGAATGGGAACATAAGACAGTGGGCAAATATAAAATTGCAGGCTTAAATTCAGATGAAGGATGGGAAATGATGAAGGCAATTGAGGATGATATAAGATCTAATTGGCTTGATATGGTTTCTTGTTCAAGGAAAATATCAAAAAAGGACCTTCTAACTTTTTTCGGTAGTGAGGATTTTCATGAAACAAAAAATTGGATAGATTGTGGATTAGCTTCAGGGTTAATGGATGGTGACAAGATTATTATGGGTCTCAGTAAGAGATTTGGACCACCGCTCGACTTCAAAATATATTCACAAGTTAAAAAGTGCACTGTTGGTATATCAAATAGTAGGGAAAATATCGGTATTATTAGAGTTTGTGGAGCCATCGATTATGGAGGTGATATAATGGTTCATGATTTGATTGAGAATATCCGTAGGGTTGGGTCATCAAAAAGTTTGAAGGCTATAATCCTACGGATTGACAGTCCAGGAGGGAATGTATTGTTGGCTGATCAATTATGGAGAGAAATCAACATTCTTGCATCTAAGATACCGGTCATTTCTTCCTTATCAGATGAGGCAGCCAGTGGTGCATATTTGATTGCAACTGGAGCAGGAACAATTGTAGCTGAGAAACTTACTATTATAGGTTCAATTGGAGTGAGACTGTCACGTGCAAACATTAAGAAAATATATGAATATTTTGGTTTTAAGAAAGAGGTTCTTTCTAAAGGTAAATATGCAGAACTATTTGAGAATGATCATCGGCTACTTATGCCACATGAAGAAGAGCGCATCAAAAATGAGTTACAAGAAATTTACAAACACTTTGTAGACAAGGTAGCGATGGCCAGATCATTAAGTGTAGATGAGGTTGAAGAGATTGCCCAAGGGCGACTTTGGACCGGTAAGGCAGCAATGAACAATAGGTTGGTTGATGCGCTAGGTGGATTATCACGTGCTGTTGCTATAGCCAAATTGAAGGCTAATATACCTCAAAATAGGCCTATTAATCTGGTTGAACTCTCTGTGTAA

Protein sequence

>MS.gene060135.t1

MTILRNMYRKISRSSSIANHSHFISHPLHLTETPQIHKPLKFHCTPSSIVDRFKSFRPIHRPFTFYFSSTSTPGKGNSVDYSTGDFDFVPLSGFKKYMVKIKTLLASERVEHGSVLRINLEGKFSDGPVGTDSQVLSLPQLSENLMKAAYDPRVSALFVYIDAFDCGWGKADEIRRQIFNFKKSGKKVIAFMPTFWGFESGVWYQNKSYYIASECHEIYAPPTSTISSPETTTLTKITKRSLKKDYTNLIEWEHKTVGKYKIAGLNSDEGWEMMKAIEDDIRSNWLDMVSCSRKISKKDLLTFFGSEDFHETKNWIDCGLASGLMDGDKIIMGLSKRFGPPLDFKIYSQVKKCTVGISNSRENIGIIRVCGAIDYGGDIMVHDLIENIRRVGSSKSLKAIILRIDSPGGNVLLADQLWREINILASKIPVISSLSDEAASGAYLIATGAGTIVAEKLTIIGSIGVRLSRANIKKIYEYFGFKKEVLSKGKYAELFENDHRLLMPHEEERIKNELQEIYKHFVDKVAMARSLSVDEVEEIAQGRLWTGKAAMNNRLVDALGGLSRAVAIAKLKANIPQNRPINLVELSV