AlfalfaGEDB | MS.gene31668

M. sativa cultivar XinJiangDaYe / MS.gene31668

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31668.t1	XP_013461819.1	95.5	88	2	1	1	88	1	86	6.00E-39	169.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31668.t1	Q9ZQB9	58.7	92	27	3	1	87	1	86	8.3e-21	100.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31668.t1	A0A072V1K1	95.5	88	2	1	1	88	1	86	4.4e-39	169.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene31668	0.810689	1.02E-50	-1.69E-46
MS.gene049887	MS.gene31668	0.804507	2.09E-49	-1.69E-46
MS.gene050551	MS.gene31668	0.821647	3.61E-53	-1.69E-46
MS.gene051102	MS.gene31668	0.823159	1.61E-53	-1.69E-46
MS.gene052514	MS.gene31668	0.812023	5.22E-51	-1.69E-46
MS.gene052516	MS.gene31668	0.826643	2.42E-54	-1.69E-46
MS.gene052609	MS.gene31668	0.812777	3.57E-51	-1.69E-46
MS.gene052665	MS.gene31668	0.824248	8.94E-54	-1.69E-46
MS.gene052732	MS.gene31668	0.803555	3.30E-49	-1.69E-46
MS.gene053143	MS.gene31668	0.822814	1.94E-53	-1.69E-46
MS.gene053144	MS.gene31668	0.831875	1.30E-55	-1.69E-46
MS.gene053581	MS.gene31668	0.80887	2.50E-50	-1.69E-46
MS.gene054584	MS.gene31668	0.820219	7.70E-53	-1.69E-46
MS.gene054824	MS.gene31668	0.807063	6.07E-50	-1.69E-46
MS.gene055465	MS.gene31668	0.843985	1.00E-58	-1.69E-46
MS.gene055466	MS.gene31668	0.809065	2.27E-50	-1.69E-46
MS.gene055467	MS.gene31668	0.83641	9.51E-57	-1.69E-46
MS.gene055468	MS.gene31668	0.805277	1.44E-49	-1.69E-46
MS.gene055469	MS.gene31668	0.839052	2.00E-57	-1.69E-46
MS.gene056308	MS.gene31668	0.818243	2.17E-52	-1.69E-46
MS.gene056507	MS.gene31668	0.828795	7.37E-55	-1.69E-46
MS.gene057446	MS.gene31668	0.812636	3.84E-51	-1.69E-46
MS.gene058647	MS.gene31668	0.816943	4.26E-52	-1.69E-46
MS.gene061006	MS.gene31668	0.827691	1.36E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31668.t1	MTR_3g103500	95.455	88	2	1	1	88	1	86	2.03e-54	176
MS.gene31668.t1	MTR_3g103500	95.455	88	2	1	1	88	1	86	1.11e-53	177
MS.gene31668.t1	MTR_3g109040	45.161	93	35	3	1	88	1	82	1.99e-18	79.0
MS.gene31668.t1	MTR_3g109040	46.914	81	30	2	10	88	2	71	5.75e-17	74.7
MS.gene31668.t1	MTR_4g006010	37.778	90	35	3	1	78	1	81	6.87e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31668.t1	AT4G07960	58.696	92	27	3	1	87	1	86	2.16e-27	104
MS.gene31668.t1	AT4G07960	58.696	92	27	3	1	87	1	86	2.16e-27	104
MS.gene31668.t1	AT4G31590	50.538	93	30	3	1	88	1	82	8.35e-23	91.3
MS.gene31668.t1	AT2G24630	49.451	91	34	2	1	88	1	82	9.49e-22	88.6
MS.gene31668.t1	AT2G24630	49.451	91	34	2	1	88	1	82	9.49e-22	88.6

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCAGCTCTATGAGCTTTA+AGG	0.164902	1.1:-12053499	None:intergenic
AGTTCATCAATGTCCCAATT+TGG	0.224089	1.1:-12053371	None:intergenic
CAAATTGCTCCTGCATTGTT+TGG	0.262621	1.1:+12053533	MS.gene31668:CDS
AAATGGCACCTTTGTTCAAT+TGG	0.282770	1.1:+12053297	None:intergenic
AATGGCACCTTTGTTCAATT+GGG	0.361924	1.1:+12053298	None:intergenic
CTTAAAGCTCATAGAGCTGC+TGG	0.464353	1.1:+12053500	MS.gene31668:CDS
TCTTCAAGAGACAAAGGAAA+AGG	0.476352	1.1:+12053443	MS.gene31668:CDS
TGGGACATTGATGAACTTGA+AGG	0.483005	1.1:+12053377	MS.gene31668:CDS
AAAAGTAAATCTTCTTCTGA+AGG	0.511562	1.1:-12053401	None:intergenic
TTAGTTATCGACGGTGGTGT+TGG	0.532054	1.1:+12053557	MS.gene31668:CDS
TTGTTTGGTTTAGTTATCGA+CGG	0.536069	1.1:+12053548	MS.gene31668:CDS
GTTATCGACGGTGGTGTTGG+TGG	0.546040	1.1:+12053560	MS.gene31668:CDS
ATGGCACCTTTGTTCAATTG+GGG	0.552161	1.1:+12053299	MS.gene31668:CDS
AAAACGCTAAACAACTCACA+TGG	0.573149	1.1:+12053468	MS.gene31668:CDS
AATTCTTCTTCAAGAGACAA+AGG	0.578590	1.1:+12053437	MS.gene31668:CDS
TTCGCACCCCAATTGAACAA+AGG	0.603697	1.1:-12053305	None:intergenic
ATAACTAAACCAAACAATGC+AGG	0.606605	1.1:-12053542	None:intergenic
TTTGGTTTAGTTATCGACGG+TGG	0.654964	1.1:+12053551	MS.gene31668:CDS
AAACGCTAAACAACTCACAT+GGG	0.684108	1.1:+12053469	MS.gene31668:CDS
GGTGCGAAAGAAAGTCACAA+AGG	0.727517	1.1:+12053320	MS.gene31668:CDS
AAGTCACAAAGGAACACCAG+TGG	0.729436	1.1:+12053331	MS.gene31668:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAATGGAGAATCCAAATT+GGG	+	chr1.1:12053358-12053377	MS.gene31668:CDS	25.0%
!	AAAAGTAAATCTTCTTCTGA+AGG	-	chr1.1:12053404-12053423	None:intergenic	25.0%
!	TAAAAATGGAGAATCCAAAT+TGG	+	chr1.1:12053357-12053376	MS.gene31668:CDS	25.0%
!!!	TCTCTTGAAGAAGAATTTTT+TGG	-	chr1.1:12053434-12053453	None:intergenic	25.0%
	AATTCTTCTTCAAGAGACAA+AGG	+	chr1.1:12053437-12053456	MS.gene31668:CDS	30.0%
	ATAACTAAACCAAACAATGC+AGG	-	chr1.1:12053545-12053564	None:intergenic	30.0%
!	TTGTTTGGTTTAGTTATCGA+CGG	+	chr1.1:12053548-12053567	MS.gene31668:CDS	30.0%
	AAAACGCTAAACAACTCACA+TGG	+	chr1.1:12053468-12053487	MS.gene31668:CDS	35.0%
	AAACGCTAAACAACTCACAT+GGG	+	chr1.1:12053469-12053488	MS.gene31668:CDS	35.0%
	AACACCAGTGGTTGTAAAAA+TGG	+	chr1.1:12053343-12053362	MS.gene31668:CDS	35.0%
	AGTTCATCAATGTCCCAATT+TGG	-	chr1.1:12053374-12053393	None:intergenic	35.0%
	TCTTCAAGAGACAAAGGAAA+AGG	+	chr1.1:12053443-12053462	MS.gene31668:CDS	35.0%
!!	TTCTCCATTTTTACAACCAC+TGG	-	chr1.1:12053350-12053369	None:intergenic	35.0%
	ATGGCACCTTTGTTCAATTG+GGG	+	chr1.1:12053299-12053318	MS.gene31668:CDS	40.0%
	CAAATTGCTCCTGCATTGTT+TGG	+	chr1.1:12053533-12053552	MS.gene31668:CDS	40.0%
	TGGGACATTGATGAACTTGA+AGG	+	chr1.1:12053377-12053396	MS.gene31668:CDS	40.0%
!	TTTGGTTTAGTTATCGACGG+TGG	+	chr1.1:12053551-12053570	MS.gene31668:CDS	40.0%
	AAGTCACAAAGGAACACCAG+TGG	+	chr1.1:12053331-12053350	MS.gene31668:CDS	45.0%
	CAGCAGCTCTATGAGCTTTA+AGG	-	chr1.1:12053502-12053521	None:intergenic	45.0%
	CTTAAAGCTCATAGAGCTGC+TGG	+	chr1.1:12053500-12053519	MS.gene31668:CDS	45.0%
	GGTGCGAAAGAAAGTCACAA+AGG	+	chr1.1:12053320-12053339	MS.gene31668:CDS	45.0%
	TTAGTTATCGACGGTGGTGT+TGG	+	chr1.1:12053557-12053576	MS.gene31668:CDS	45.0%
	TTCGCACCCCAATTGAACAA+AGG	-	chr1.1:12053308-12053327	None:intergenic	45.0%
!	GTTATCGACGGTGGTGTTGG+TGG	+	chr1.1:12053560-12053579	MS.gene31668:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	12053299	12053595	12053299	ID=MS.gene31668
chr1.1	mRNA	12053299	12053595	12053299	ID=MS.gene31668.t1;Parent=MS.gene31668
chr1.1	exon	12053299	12053595	12053299	ID=MS.gene31668.t1.exon1;Parent=MS.gene31668.t1
chr1.1	CDS	12053299	12053595	12053299	ID=cds.MS.gene31668.t1;Parent=MS.gene31668.t1

Gene Sequence	>MS.gene31668 ATGGCACCTTTGTTCAATTGGGGTGCGAAAGAAAGTCACAAAGGAACACCAGTGGTTGTAAAAATGGAGAATCCAAATTGGGACATTGATGAACTTGAAGGTCCTTCAGAAGAAGATTTACTTTTAACAACACCAAAAAATTCTTCTTCAAGAGACAAAGGAAAAGGCAAAAACGCTAAACAACTCACATGGGTTCTTCTCCTTAAAGCTCATAGAGCTGCTGGTTGTTTAGCTCAAATTGCTCCTGCATTGTTTGGTTTAGTTATCGACGGTGGTGTTGGTGGTGTAAAGCATTGA
Protein sequence	>MS.gene31668.t1 MAPLFNWGAKESHKGTPVVVKMENPNWDIDELEGPSEEDLLLTTPKNSSSRDKGKGKNAKQLTWVLLLKAHRAAGCLAQIAPALFGLVIDGGVGGVKH