Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32571.t1 | AES99351.1 | 99.7 | 308 | 1 | 0 | 1 | 308 | 1 | 308 | 6.50E-172 | 613.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32571.t1 | Q8W1X2 | 84.3 | 306 | 48 | 0 | 3 | 308 | 4 | 309 | 4.3e-148 | 525.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32571.t1 | G7KBP8 | 99.7 | 308 | 1 | 0 | 1 | 308 | 1 | 308 | 4.7e-172 | 613.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene32571 | -0.80635 | 8.59E-50 | -1.69E-46 |
| MS.gene049368 | MS.gene32571 | -0.813564 | 2.40E-51 | -1.69E-46 |
| MS.gene050838 | MS.gene32571 | -0.818772 | 1.65E-52 | -1.69E-46 |
| MS.gene050941 | MS.gene32571 | -0.806976 | 6.33E-50 | -1.69E-46 |
| MS.gene051234 | MS.gene32571 | -0.804753 | 1.86E-49 | -1.69E-46 |
| MS.gene052296 | MS.gene32571 | -0.80658 | 7.68E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene32571 | -0.804 | 2.66E-49 | -1.69E-46 |
| MS.gene054004 | MS.gene32571 | -0.805916 | 1.06E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene32571 | 0.849652 | 2.82E-60 | -1.69E-46 |
| MS.gene057893 | MS.gene32571 | 0.811624 | 6.38E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene32571 | -0.8028 | 4.72E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene32571 | -0.84501 | 5.30E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32571.t1 | MTR_5g079730 | 99.675 | 308 | 1 | 0 | 1 | 308 | 43 | 350 | 0.0 | 627 |
| MS.gene32571.t1 | MTR_5g079730 | 99.675 | 308 | 1 | 0 | 1 | 308 | 1 | 308 | 0.0 | 627 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32571.t1 | AT5G37850 | 84.039 | 307 | 49 | 0 | 2 | 308 | 3 | 309 | 0.0 | 536 |
| MS.gene32571.t1 | AT5G37850 | 84.039 | 307 | 49 | 0 | 2 | 308 | 3 | 309 | 0.0 | 536 |
| MS.gene32571.t1 | AT5G37850 | 84.039 | 307 | 49 | 0 | 2 | 308 | 37 | 343 | 0.0 | 535 |
| MS.gene32571.t1 | AT5G37850 | 77.199 | 307 | 42 | 1 | 2 | 308 | 37 | 315 | 6.51e-170 | 474 |
Find 78 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTAAAATAAGCTGGTATTT+TGG | 0.109128 | 5.1:-61968537 | None:intergenic |
| GAAAACTGCACGGAATTAAT+TGG | 0.219716 | 5.1:-61962015 | None:intergenic |
| AATTGCAATCTTAAATTGTT+TGG | 0.234230 | 5.1:-61968513 | None:intergenic |
| GATCTCTAAACTGGATGATT+TGG | 0.234798 | 5.1:-61969424 | None:intergenic |
| GCTGAGTACTCGACCGGTTT+CGG | 0.286157 | 5.1:-61961561 | None:intergenic |
| TACTATACTCACTTATTAAC+AGG | 0.313318 | 5.1:+61962339 | MS.gene32571:CDS |
| CATTCAATCTCACACTGTTC+AGG | 0.316627 | 5.1:+61961583 | MS.gene32571:CDS |
| ATTACAAGTATAAATGTAGA+TGG | 0.322665 | 5.1:+61967434 | MS.gene32571:CDS |
| ATTGCAATCTTAAATTGTTT+GGG | 0.334187 | 5.1:-61968512 | None:intergenic |
| AATTAACCTGATCTCTAAAC+TGG | 0.348609 | 5.1:-61969433 | None:intergenic |
| CTCATGACCGCACTTCTTCT+CGG | 0.358402 | 5.1:+61968976 | MS.gene32571:CDS |
| ATCTCACCATATGTAAGCTT+GGG | 0.360323 | 5.1:-61963919 | None:intergenic |
| ACAATAAATCATTGGCTTCA+AGG | 0.365121 | 5.1:-61962317 | None:intergenic |
| TTTCCTTCATCACCCAATAC+TGG | 0.366568 | 5.1:-61965548 | None:intergenic |
| AATTCTGCTTCAAACTGGTT+GGG | 0.370817 | 5.1:-61965904 | None:intergenic |
| GCAGCCTTCTCAAGGTTGTC+CGG | 0.375285 | 5.1:-61969116 | None:intergenic |
| TTAGCTCTCCCTTCCGAAAC+CGG | 0.402158 | 5.1:+61961548 | MS.gene32571:CDS |
| ATCTCTAAACTGGATGATTT+GGG | 0.402474 | 5.1:-61969423 | None:intergenic |
| TATTTGTAGCAAACCTTTGA+AGG | 0.414189 | 5.1:-61966951 | None:intergenic |
| GATGGGAATCTTCTCCTCAT+TGG | 0.430577 | 5.1:+61967452 | MS.gene32571:CDS |
| GAATTGAGTTACAAGCTTCT+CGG | 0.437898 | 5.1:-61966917 | None:intergenic |
| AATCATCCAGTTTAGAGATC+AGG | 0.438326 | 5.1:+61969427 | MS.gene32571:CDS |
| TCAGTAATTCTGCTTCAAAC+TGG | 0.439746 | 5.1:-61965909 | None:intergenic |
| AAAACCTGGCCCTTAAAAGA+TGG | 0.449977 | 5.1:-61962261 | None:intergenic |
| ATTCTGCTTCAAACTGGTTG+GGG | 0.465962 | 5.1:-61965903 | None:intergenic |
| TAATTCTGCTTCAAACTGGT+TGG | 0.476012 | 5.1:-61965905 | None:intergenic |
| TCAGCTTTGAATGTGATTTG+TGG | 0.476834 | 5.1:-61969480 | None:intergenic |
| AATCTCACCATATGTAAGCT+TGG | 0.477830 | 5.1:-61963920 | None:intergenic |
| TGACCGCACTTCTTCTCGGT+TGG | 0.480032 | 5.1:+61968980 | MS.gene32571:CDS |
| CAGCCTTCTCAAGGTTGTCC+GGG | 0.480511 | 5.1:-61969115 | None:intergenic |
| ATAAACCCCAAGCTTACATA+TGG | 0.480838 | 5.1:+61963913 | MS.gene32571:CDS |
| GTCAGTATGTGATCCAGTAT+TGG | 0.485806 | 5.1:+61965535 | MS.gene32571:intron |
| TCAGTATGTGATCCAGTATT+GGG | 0.487775 | 5.1:+61965536 | MS.gene32571:intron |
| GTGGACTGCGGATATCATCT+TGG | 0.488939 | 5.1:-61969461 | None:intergenic |
| GGGGTTTATTGAGCGGAGCT+TGG | 0.489167 | 5.1:-61963899 | None:intergenic |
| TCATGCAGCTGGACCTTCAA+AGG | 0.493732 | 5.1:+61966938 | MS.gene32571:CDS |
| GGGGTCAACATTGAAGCGAT+TGG | 0.494460 | 5.1:-61965883 | None:intergenic |
| TATTACTCTCAGGGTTATGT+TGG | 0.500380 | 5.1:+61961943 | MS.gene32571:intron |
| CCTCATTGGAAGCCATCTAA+AGG | 0.505751 | 5.1:+61967466 | MS.gene32571:CDS |
| TGTAACTCAATTCATGCAGC+TGG | 0.505953 | 5.1:+61966927 | MS.gene32571:CDS |
| CAACTCTGGGATCTAATTGA+AGG | 0.506637 | 5.1:+61962294 | MS.gene32571:CDS |
| AGTATAGTACAATAAATCAT+TGG | 0.506948 | 5.1:-61962325 | None:intergenic |
| CGAGTTCTGCAGCCTTCTCA+AGG | 0.509699 | 5.1:-61969124 | None:intergenic |
| TGGAAGCCATCTAAAGGAAA+AGG | 0.511024 | 5.1:+61967472 | MS.gene32571:CDS |
| TGCTTGCTTGCTTGCCTGTG+TGG | 0.511262 | 5.1:-61962041 | None:intergenic |
| GCTTTATGTTCCACAAGAGC+TGG | 0.512326 | 5.1:+61965571 | MS.gene32571:CDS |
| CTTGATGATTACAAAAGTGC+TGG | 0.513356 | 5.1:+61969393 | MS.gene32571:CDS |
| TACACTGTCACCAGCTCTTG+TGG | 0.526610 | 5.1:-61965581 | None:intergenic |
| CCTTTAGATGGCTTCCAATG+AGG | 0.528708 | 5.1:-61967466 | None:intergenic |
| ATTCATACCGTAAAATAAGC+TGG | 0.529696 | 5.1:-61968545 | None:intergenic |
| GGGAGAGCTAAGGAAAGAAT+CGG | 0.537810 | 5.1:-61961536 | None:intergenic |
| GCAATCTTAAATTGTTTGGG+AGG | 0.538067 | 5.1:-61968509 | None:intergenic |
| TTACAAGTATAAATGTAGAT+GGG | 0.542416 | 5.1:+61967435 | MS.gene32571:CDS |
| TTGAATGCTGAGTACTCGAC+CGG | 0.551447 | 5.1:-61961567 | None:intergenic |
| GTTGCTGTCCATTTAAAACC+TGG | 0.567429 | 5.1:-61962275 | None:intergenic |
| AGGATTCAGTCTGAAGAAGA+TGG | 0.568689 | 5.1:+61966894 | MS.gene32571:intron |
| TTTAAATGGACAGCAACTCT+GGG | 0.571824 | 5.1:+61962281 | MS.gene32571:CDS |
| AGTACTCGACCGGTTTCGGA+AGG | 0.577273 | 5.1:-61961557 | None:intergenic |
| CAGAATTACTGACAGGACTC+AGG | 0.582541 | 5.1:+61965920 | MS.gene32571:CDS |
| ACTCGCGGTATCAAGCGTAC+AGG | 0.589125 | 5.1:+61969142 | MS.gene32571:CDS |
| TGGTGACAGTGTACCGTGAG+AGG | 0.589337 | 5.1:+61965591 | MS.gene32571:CDS |
| GATCCAGTATTGGGTGATGA+AGG | 0.594822 | 5.1:+61965545 | MS.gene32571:CDS |
| TAAGCTTGGGGTTTATTGAG+CGG | 0.599072 | 5.1:-61963906 | None:intergenic |
| TTTGAAGCAGAATTACTGAC+AGG | 0.602351 | 5.1:+61965913 | MS.gene32571:CDS |
| TTCTGCTTCAAACTGGTTGG+GGG | 0.607738 | 5.1:-61965902 | None:intergenic |
| GTACTCGACCGGTTTCGGAA+GGG | 0.612979 | 5.1:-61961556 | None:intergenic |
| TCTCACCATATGTAAGCTTG+GGG | 0.617919 | 5.1:-61963918 | None:intergenic |
| GGATCAGTCTTACCCTCTCA+CGG | 0.627957 | 5.1:-61965604 | None:intergenic |
| GGTGACAGTGTACCGTGAGA+GGG | 0.631189 | 5.1:+61965592 | MS.gene32571:CDS |
| TCATAGCCCAAAAGTTGCAG+AGG | 0.634972 | 5.1:-61961985 | None:intergenic |
| TCATTCGTTTCAGAAACACC+CGG | 0.638652 | 5.1:+61969097 | MS.gene32571:intron |
| TGAGAAGGCTGCAGAACTCG+CGG | 0.646112 | 5.1:+61969127 | MS.gene32571:CDS |
| ACACCCGGACAACCTTGAGA+AGG | 0.658420 | 5.1:+61969112 | MS.gene32571:CDS |
| GGTTTCGGAAGGGAGAGCTA+AGG | 0.664576 | 5.1:-61961546 | None:intergenic |
| TGAATGTGATTTGTGGACTG+CGG | 0.665592 | 5.1:-61969473 | None:intergenic |
| AGAGCTAAGGAAAGAATCGG+AGG | 0.674260 | 5.1:-61961533 | None:intergenic |
| GCTCCAACCGAGAAGAAGTG+CGG | 0.693199 | 5.1:-61968983 | None:intergenic |
| TGTGTGGTTTGAAAACTGCA+CGG | 0.733496 | 5.1:-61962025 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 61961527 | 61969515 | 61961527 | ID=MS.gene32571 |
| chr5.1 | mRNA | 61961527 | 61969515 | 61961527 | ID=MS.gene32571.t1;Parent=MS.gene32571 |
| chr5.1 | exon | 61961527 | 61961604 | 61961527 | ID=MS.gene32571.t1.exon1;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61961527 | 61961604 | 61961527 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61961955 | 61962048 | 61961955 | ID=MS.gene32571.t1.exon2;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61961955 | 61962048 | 61961955 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61962256 | 61962360 | 61962256 | ID=MS.gene32571.t1.exon3;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61962256 | 61962360 | 61962256 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61963851 | 61963934 | 61963851 | ID=MS.gene32571.t1.exon4;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61963851 | 61963934 | 61963851 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61965540 | 61965613 | 61965540 | ID=MS.gene32571.t1.exon5;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61965540 | 61965613 | 61965540 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61965877 | 61965941 | 61965877 | ID=MS.gene32571.t1.exon6;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61965877 | 61965941 | 61965877 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61966896 | 61966959 | 61966896 | ID=MS.gene32571.t1.exon7;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61966896 | 61966959 | 61966896 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61967428 | 61967493 | 61967428 | ID=MS.gene32571.t1.exon8;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61967428 | 61967493 | 61967428 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61968503 | 61968559 | 61968503 | ID=MS.gene32571.t1.exon9;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61968503 | 61968559 | 61968503 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61968964 | 61969008 | 61968964 | ID=MS.gene32571.t1.exon10;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61968964 | 61969008 | 61968964 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61969110 | 61969163 | 61969110 | ID=MS.gene32571.t1.exon11;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61969110 | 61969163 | 61969110 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| chr5.1 | exon | 61969375 | 61969515 | 61969375 | ID=MS.gene32571.t1.exon12;Parent=MS.gene32571.t1 |
| chr5.1 | CDS | 61969375 | 61969515 | 61969375 | ID=cds.MS.gene32571.t1;Parent=MS.gene32571.t1 |
| Gene Sequence |
| Protein sequence |
