Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058261.t1 | XP_003594704.1 | 96.8 | 222 | 7 | 0 | 1 | 222 | 1 | 222 | 1.20E-55 | 226.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058261.t1 | A2Q675 | 96.8 | 222 | 7 | 0 | 1 | 222 | 1 | 222 | 9.0e-56 | 226.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050752 | MS.gene058261 | 0.818876 | 1.56E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene058261 | 0.840857 | 6.75E-58 | -1.69E-46 |
| MS.gene051003 | MS.gene058261 | 0.801454 | 8.94E-49 | -1.69E-46 |
| MS.gene051233 | MS.gene058261 | 0.804085 | 2.56E-49 | -1.69E-46 |
| MS.gene051234 | MS.gene058261 | 0.84887 | 4.65E-60 | -1.69E-46 |
| MS.gene051902 | MS.gene058261 | 0.821103 | 4.82E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene058261 | 0.803874 | 2.83E-49 | -1.69E-46 |
| MS.gene053058 | MS.gene058261 | 0.802162 | 6.39E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene058261 | 0.820223 | 7.69E-53 | -1.69E-46 |
| MS.gene056752 | MS.gene058261 | 0.807655 | 4.55E-50 | -1.69E-46 |
| MS.gene056801 | MS.gene058261 | 0.822028 | 2.95E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene060763 | 0.800185 | 1.62E-48 | -1.69E-46 |
| MS.gene058261 | MS.gene061100 | 0.803306 | 3.71E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene061878 | 0.848142 | 7.40E-60 | -1.69E-46 |
| MS.gene058261 | MS.gene06238 | 0.808214 | 3.46E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene062770 | 0.804025 | 2.63E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene065055 | 0.815263 | 1.01E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene06729 | 0.809514 | 1.82E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene068166 | 0.812591 | 3.93E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene068492 | 0.809845 | 1.55E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene068891 | 0.807129 | 5.88E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene06946 | 0.806536 | 7.84E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene069828 | 0.828115 | 1.08E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene06984 | 0.819387 | 1.19E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene06998 | 0.821876 | 3.20E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene070813 | 0.807529 | 4.83E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene071074 | 0.80541 | 1.35E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene072328 | 0.807344 | 5.29E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene072329 | 0.808903 | 2.46E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene072555 | 0.805423 | 1.34E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene072641 | 0.847516 | 1.10E-59 | -1.69E-46 |
| MS.gene058261 | MS.gene072740 | 0.8132 | 2.89E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene07878 | 0.814722 | 1.33E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene08793 | 0.858364 | 8.67E-63 | -1.69E-46 |
| MS.gene058261 | MS.gene08889 | 0.83704 | 6.57E-57 | -1.69E-46 |
| MS.gene058261 | MS.gene08922 | 0.806815 | 6.85E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene08948 | 0.80107 | 1.07E-48 | -1.69E-46 |
| MS.gene058261 | MS.gene09297 | 0.849053 | 4.14E-60 | -1.69E-46 |
| MS.gene058261 | MS.gene20064 | 0.813039 | 3.13E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene21103 | 0.813175 | 2.92E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene21105 | 0.813601 | 2.36E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene21107 | 0.813736 | 2.20E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene21541 | 0.837595 | 4.74E-57 | -1.69E-46 |
| MS.gene058261 | MS.gene21651 | 0.809708 | 1.66E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene21680 | 0.808826 | 2.56E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene22288 | 0.856307 | 3.52E-62 | -1.69E-46 |
| MS.gene058261 | MS.gene22321 | 0.825767 | 3.92E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene22433 | 0.81488 | 1.23E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene23060 | 0.820679 | 6.04E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene23935 | 0.838199 | 3.31E-57 | -1.69E-46 |
| MS.gene058261 | MS.gene24907 | 0.825579 | 4.34E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene25061 | 0.852896 | 3.42E-61 | -1.69E-46 |
| MS.gene058261 | MS.gene25901 | 0.828337 | 9.51E-55 | -1.69E-46 |
| MS.gene058261 | MS.gene25923 | 0.802058 | 6.72E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene25981 | -0.834731 | 2.53E-56 | -1.69E-46 |
| MS.gene058261 | MS.gene26426 | 0.801164 | 1.02E-48 | -1.69E-46 |
| MS.gene058261 | MS.gene26605 | 0.825599 | 4.29E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene28409 | 0.838294 | 3.13E-57 | -1.69E-46 |
| MS.gene058261 | MS.gene28632 | 0.846367 | 2.27E-59 | -1.69E-46 |
| MS.gene058261 | MS.gene28820 | 0.845916 | 3.01E-59 | -1.69E-46 |
| MS.gene058261 | MS.gene29355 | 0.834611 | 2.71E-56 | -1.69E-46 |
| MS.gene058261 | MS.gene29700 | 0.824788 | 6.68E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene29794 | 0.826298 | 2.93E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene31045 | 0.805514 | 1.29E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene32571 | -0.8028 | 4.72E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene32688 | 0.831109 | 2.01E-55 | -1.69E-46 |
| MS.gene058261 | MS.gene33250 | 0.839783 | 1.29E-57 | -1.69E-46 |
| MS.gene058261 | MS.gene34508 | 0.806718 | 7.18E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene35034 | 0.804805 | 1.81E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene36081 | 0.801709 | 7.92E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene36497 | 0.81419 | 1.75E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene37236 | 0.813488 | 2.50E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene37238 | 0.81229 | 4.57E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene37345 | -0.80559 | 1.24E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene38070 | 0.802881 | 4.55E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene40055 | 0.824296 | 8.72E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene40220 | 0.83569 | 1.45E-56 | -1.69E-46 |
| MS.gene058261 | MS.gene41088 | 0.820706 | 5.95E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene41681 | 0.813537 | 2.43E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene42653 | 0.824297 | 8.71E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene44141 | 0.803196 | 3.91E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene44240 | 0.820361 | 7.15E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene44631 | 0.816244 | 6.11E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene45281 | 0.80624 | 9.06E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene45785 | 0.824649 | 7.20E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene47674 | 0.812638 | 3.83E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene47995 | 0.819252 | 1.28E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene48742 | 0.80463 | 1.97E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene49047 | 0.81975 | 9.86E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene50005 | 0.803646 | 3.16E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene51445 | 0.838418 | 2.91E-57 | -1.69E-46 |
| MS.gene058261 | MS.gene51641 | 0.815482 | 9.04E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene51704 | 0.802247 | 6.14E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene52281 | 0.804919 | 1.71E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene52837 | 0.826399 | 2.77E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene53552 | 0.818487 | 1.91E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene54299 | 0.802807 | 4.71E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene55094 | 0.804919 | 1.71E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene55530 | 0.838417 | 2.91E-57 | -1.69E-46 |
| MS.gene058261 | MS.gene56163 | 0.830086 | 3.58E-55 | -1.69E-46 |
| MS.gene058261 | MS.gene56742 | 0.826169 | 3.14E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene57783 | 0.807758 | 4.32E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene57980 | 0.810625 | 1.05E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene58098 | 0.861471 | 1.00E-63 | -1.69E-46 |
| MS.gene058261 | MS.gene58227 | 0.823893 | 1.08E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene58925 | 0.83128 | 1.83E-55 | -1.69E-46 |
| MS.gene058261 | MS.gene59241 | 0.826281 | 2.96E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene59242 | 0.80783 | 4.17E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene59505 | 0.810822 | 9.52E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene60093 | 0.812144 | 4.91E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene61001 | 0.801928 | 7.14E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene61282 | 0.81366 | 2.29E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene61644 | 0.825164 | 5.44E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene61928 | 0.804412 | 2.19E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene62423 | 0.803754 | 3.00E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene62834 | 0.818117 | 2.32E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene63726 | 0.808755 | 2.65E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene64806 | 0.812969 | 3.24E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene67010 | 0.803907 | 2.79E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene67412 | 0.82013 | 8.07E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene67881 | 0.824433 | 8.09E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene68713 | 0.803454 | 3.46E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene68735 | 0.814737 | 1.32E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene69097 | 0.818437 | 1.96E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene70175 | 0.808397 | 3.16E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene70577 | 0.831587 | 1.53E-55 | -1.69E-46 |
| MS.gene058261 | MS.gene70596 | 0.802109 | 6.56E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene71994 | 0.854079 | 1.56E-61 | -1.69E-46 |
| MS.gene058261 | MS.gene72285 | 0.806061 | 9.87E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene72353 | 0.807812 | 4.21E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene72950 | -0.816846 | 4.48E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene73462 | 0.802239 | 6.17E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene74606 | 0.817058 | 4.02E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene74972 | 0.817115 | 3.90E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene75072 | 0.815479 | 9.05E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene76440 | 0.806404 | 8.36E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene76830 | 0.812382 | 4.36E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene77361 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene80201 | 0.825444 | 4.67E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene81280 | 0.807125 | 5.89E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene82828 | 0.821038 | 4.99E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene84677 | 0.801779 | 7.66E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene85610 | 0.858675 | 7.00E-63 | -1.69E-46 |
| MS.gene058261 | MS.gene86038 | 0.820611 | 6.26E-53 | -1.69E-46 |
| MS.gene058261 | MS.gene86061 | 0.857498 | 1.57E-62 | -1.69E-46 |
| MS.gene058261 | MS.gene86919 | 0.807678 | 4.50E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene86931 | 0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene88927 | 0.827041 | 1.95E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene89605 | 0.804607 | 1.99E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene89663 | 0.831099 | 2.02E-55 | -1.69E-46 |
| MS.gene058261 | MS.gene90965 | 0.800842 | 1.19E-48 | -1.69E-46 |
| MS.gene058261 | MS.gene91203 | 0.80647 | 8.10E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene91307 | 0.802565 | 5.28E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene92135 | 0.807241 | 5.56E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene92256 | 0.810098 | 1.36E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene92572 | 0.80513 | 1.55E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene93728 | 0.815953 | 7.10E-52 | -1.69E-46 |
| MS.gene058261 | MS.gene94952 | 0.80669 | 7.28E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene95049 | 0.813687 | 2.26E-51 | -1.69E-46 |
| MS.gene058261 | MS.gene96690 | 0.813337 | 2.69E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058261.t1 | MTR_2g033650 | 96.396 | 222 | 8 | 0 | 1 | 222 | 1 | 222 | 3.13e-155 | 429 |
| MS.gene058261.t1 | MTR_4g122710 | 52.846 | 246 | 87 | 7 | 1 | 222 | 1 | 241 | 1.29e-46 | 154 |
| MS.gene058261.t1 | MTR_1g086670 | 41.892 | 222 | 102 | 7 | 7 | 203 | 9 | 228 | 2.95e-38 | 133 |
| MS.gene058261.t1 | MTR_1g086670 | 41.892 | 222 | 102 | 7 | 7 | 203 | 40 | 259 | 8.01e-38 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058261.t1 | AT3G17120 | 39.316 | 234 | 104 | 7 | 6 | 220 | 2 | 216 | 2.80e-32 | 117 |
| MS.gene058261.t1 | AT3G17120 | 39.316 | 234 | 104 | 7 | 6 | 220 | 2 | 216 | 2.80e-32 | 117 |
| MS.gene058261.t1 | AT3G17120 | 39.316 | 234 | 104 | 7 | 6 | 220 | 2 | 216 | 2.80e-32 | 117 |
| MS.gene058261.t1 | AT3G17120 | 39.316 | 234 | 104 | 7 | 6 | 220 | 2 | 216 | 2.80e-32 | 117 |
| MS.gene058261.t1 | AT3G17120 | 39.316 | 234 | 104 | 7 | 6 | 220 | 2 | 216 | 2.80e-32 | 117 |
| MS.gene058261.t1 | AT1G02380 | 36.957 | 230 | 129 | 9 | 1 | 219 | 1 | 225 | 8.94e-31 | 113 |
| MS.gene058261.t1 | AT4G01960 | 35.000 | 240 | 130 | 10 | 1 | 222 | 1 | 232 | 1.10e-22 | 92.4 |
| MS.gene058261.t1 | AT4G01960 | 35.000 | 240 | 130 | 10 | 1 | 222 | 1 | 232 | 1.10e-22 | 92.4 |
| MS.gene058261.t1 | AT4G01960 | 41.143 | 175 | 82 | 6 | 1 | 159 | 1 | 170 | 1.88e-18 | 80.5 |
Find 46 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAGAGGAGGGTTTGATTT+CGG | 0.334177 | 2.4:+56985518 | MS.gene058261:CDS |
| GGCGCATTTGAATCACTTGA+TGG | 0.361497 | 2.4:-56985558 | None:intergenic |
| ATGATTTCTAGTGGATTTGT+TGG | 0.361904 | 2.4:+56984440 | MS.gene058261:CDS |
| AGCAGATCATAAACTAATAA+AGG | 0.364985 | 2.4:+56984643 | MS.gene058261:CDS |
| TTTGTTGGAGGTTCTGTTAA+TGG | 0.365710 | 2.4:+56984455 | MS.gene058261:CDS |
| TCTGTTCCTCCTGCTGATAC+AGG | 0.383210 | 2.4:-56985501 | None:intergenic |
| TTGTGGAGGTCGCGGAGGTT+TGG | 0.387403 | 2.4:-56984603 | None:intergenic |
| TACTTGATGGACTCCGACCC+TGG | 0.400464 | 2.4:-56985579 | None:intergenic |
| ATTGAAGTTGATATTGAAAG+TGG | 0.415838 | 2.4:+56984380 | MS.gene058261:CDS |
| TTTGCAGGCATGTCATCAAC+TGG | 0.421270 | 2.4:+56985447 | MS.gene058261:intron |
| ACCTCCACAAGGCCCGTCCT+TGG | 0.426868 | 2.4:+56984616 | MS.gene058261:CDS |
| CTTGAGTTATCTTCGATAAG+TGG | 0.433854 | 2.4:-56984407 | None:intergenic |
| GATTCATTCAAATAAGAACT+TGG | 0.458751 | 2.4:-56984488 | None:intergenic |
| GACGGGCCTTGTGGAGGTCG+CGG | 0.460222 | 2.4:-56984611 | None:intergenic |
| ATTTCTAGTGGATTTGTTGG+AGG | 0.464052 | 2.4:+56984443 | MS.gene058261:CDS |
| TTTGGGATGGATCAAAGAAA+TGG | 0.470867 | 2.4:+56984344 | None:intergenic |
| CTGCTGATACAGGTGATCCT+TGG | 0.480945 | 2.4:-56985491 | None:intergenic |
| TCAAGTGATTCAAATGCGCC+AGG | 0.485179 | 2.4:+56985561 | MS.gene058261:CDS |
| GCATCCAAGGACGGGCCTTG+TGG | 0.503446 | 2.4:-56984620 | None:intergenic |
| AGAATCTCTATGATTTCTAG+TGG | 0.517852 | 2.4:+56984431 | MS.gene058261:CDS |
| ATTCAAATAAGAACTTGGCC+TGG | 0.519471 | 2.4:-56984483 | None:intergenic |
| CAAGGATCACCTGTATCAGC+AGG | 0.531822 | 2.4:+56985492 | MS.gene058261:CDS |
| TCCAAGGACGGGCCTTGTGG+AGG | 0.539897 | 2.4:-56984617 | None:intergenic |
| GATCTGCTGCATCCAAGGAC+GGG | 0.545824 | 2.4:-56984628 | None:intergenic |
| AGCTCAGTGTCAAGTTTCCA+AGG | 0.545861 | 2.4:+56985474 | MS.gene058261:CDS |
| TTTATGATCTGCTGCATCCA+AGG | 0.547157 | 2.4:-56984633 | None:intergenic |
| TTCATTGTCATAATCTGCCA+AGG | 0.557266 | 2.4:+56984803 | MS.gene058261:CDS |
| GTTCTGTTAATGGCGAAGCC+AGG | 0.564549 | 2.4:+56984465 | MS.gene058261:CDS |
| TGATCTGCTGCATCCAAGGA+CGG | 0.564999 | 2.4:-56984629 | None:intergenic |
| TTGCGATGTTGAAGCGTGCA+AGG | 0.575727 | 2.4:+56984678 | MS.gene058261:CDS |
| TTCTGTGAATATGATGAAAG+TGG | 0.576188 | 2.4:+56984526 | MS.gene058261:CDS |
| GGATGGATCAAAGAAATGGA+AGG | 0.596860 | 2.4:+56984348 | None:intergenic |
| TGATTCAAATGCGCCAGGGT+CGG | 0.597497 | 2.4:+56985566 | MS.gene058261:CDS |
| TGTATCAGCAGGAGGAACAG+AGG | 0.606917 | 2.4:+56985503 | MS.gene058261:CDS |
| ATCAGCAGGAGGAACAGAGG+AGG | 0.609779 | 2.4:+56985506 | MS.gene058261:CDS |
| ATCTAATAGCAGCAGCATTG+TGG | 0.611367 | 2.4:+56984757 | MS.gene058261:CDS |
| GGGCCTTGTGGAGGTCGCGG+AGG | 0.611484 | 2.4:-56984608 | None:intergenic |
| AAACCTCCGCGACCTCCACA+AGG | 0.611809 | 2.4:+56984605 | MS.gene058261:CDS |
| AATCACTTGATGGATTGAGT+TGG | 0.614440 | 2.4:-56985548 | None:intergenic |
| CACTTATCGAAGATAACTCA+AGG | 0.618096 | 2.4:+56984408 | MS.gene058261:CDS |
| ATGCTGCTGCTATTAGATGA+CGG | 0.623369 | 2.4:-56984752 | None:intergenic |
| TATGATGAAAGTGGTGAACA+AGG | 0.632488 | 2.4:+56984535 | MS.gene058261:CDS |
| TCAGCAGGAGGAACAGAGGA+GGG | 0.636538 | 2.4:+56985507 | MS.gene058261:CDS |
| GATGGATCAAAGAAATGGAA+GGG | 0.642676 | 2.4:+56984349 | None:intergenic |
| CAAGTGATTCAAATGCGCCA+GGG | 0.667412 | 2.4:+56985562 | MS.gene058261:CDS |
| GGATCACCTGTATCAGCAGG+AGG | 0.681644 | 2.4:+56985495 | MS.gene058261:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACATACAAAAAATTTGGT+TGG | + | chr2.4:56985380-56985399 | MS.gene058261:intron | 20.0% |
| !! | ATTACTATCAAATTTCATCA+TGG | + | chr2.4:56985129-56985148 | MS.gene058261:intron | 20.0% |
| !! | CAACCATTAGATTAATTAAA+TGG | - | chr2.4:56985690-56985709 | None:intergenic | 20.0% |
| !! | TTCCAAACATACAAAAAATT+TGG | + | chr2.4:56985376-56985395 | MS.gene058261:intron | 20.0% |
| !!! | AACCAAATTTTTTGTATGTT+TGG | - | chr2.4:56985381-56985400 | None:intergenic | 20.0% |
| !!! | AATTTTTTGTATGTTTGGAA+TGG | - | chr2.4:56985376-56985395 | None:intergenic | 20.0% |
| ! | AGCAGATCATAAACTAATAA+AGG | + | chr2.4:56984643-56984662 | MS.gene058261:CDS | 25.0% |
| ! | GATTCATTCAAATAAGAACT+TGG | - | chr2.4:56984491-56984510 | None:intergenic | 25.0% |
| ! | TCGCCATTTAATTAATCTAA+TGG | + | chr2.4:56985684-56985703 | MS.gene058261:intron | 25.0% |
| !! | AATTTTATATGGCCTAATAG+AGG | - | chr2.4:56984975-56984994 | None:intergenic | 25.0% |
| !!! | ATTGAAGTTGATATTGAAAG+TGG | + | chr2.4:56984380-56984399 | MS.gene058261:CDS | 25.0% |
| AGAAATGCCAAAGAAACAAT+TGG | - | chr2.4:56985160-56985179 | None:intergenic | 30.0% | |
| AGCAAAAGAAAAACGGAAAA+AGG | + | chr2.4:56984562-56984581 | MS.gene058261:CDS | 30.0% | |
| GAAACAGATTAGATATGCAA+TGG | - | chr2.4:56984953-56984972 | None:intergenic | 30.0% | |
| GATCAATATCACAACACTAA+GGG | - | chr2.4:56985637-56985656 | None:intergenic | 30.0% | |
| TCTAATCTGTTTCCTCTATT+AGG | + | chr2.4:56984960-56984979 | MS.gene058261:intron | 30.0% | |
| TTCTGTGAATATGATGAAAG+TGG | + | chr2.4:56984526-56984545 | MS.gene058261:CDS | 30.0% | |
| ! | AGAATCTCTATGATTTCTAG+TGG | + | chr2.4:56984431-56984450 | MS.gene058261:CDS | 30.0% |
| ! | TAATGGTTGAAATTTTGCGA+GGG | + | chr2.4:56985701-56985720 | MS.gene058261:intron | 30.0% |
| ! | TAATTAGAAGTTCCTTTTGC+AGG | + | chr2.4:56985432-56985451 | MS.gene058261:intron | 30.0% |
| ! | TGTGTTTTCAACTTACTTGA+TGG | - | chr2.4:56985595-56985614 | None:intergenic | 30.0% |
| ! | TTGTACCACAAATTTTACCT+TGG | - | chr2.4:56984823-56984842 | None:intergenic | 30.0% |
| !! | ATGATTTCTAGTGGATTTGT+TGG | + | chr2.4:56984440-56984459 | MS.gene058261:CDS | 30.0% |
| !! | TTTTTCCGTTTTTCTTTTGC+TGG | - | chr2.4:56984563-56984582 | None:intergenic | 30.0% |
| !! | TTTTTGTATGTTTGGAATGG+CGG | - | chr2.4:56985373-56985392 | None:intergenic | 30.0% |
| !!! | AGTTTTGTTGCATTCATGAT+TGG | + | chr2.4:56985191-56985210 | MS.gene058261:intron | 30.0% |
| !!! | GTTTGTTTTTGTGCAATGTT+TGG | + | chr2.4:56985237-56985256 | MS.gene058261:intron | 30.0% |
| ACAAAAACAAACAATACCGC+AGG | - | chr2.4:56985229-56985248 | None:intergenic | 35.0% | |
| ATCTGCCAAGGTAAAATTTG+TGG | + | chr2.4:56984815-56984834 | MS.gene058261:intron | 35.0% | |
| ATTCAAATAAGAACTTGGCC+TGG | - | chr2.4:56984486-56984505 | None:intergenic | 35.0% | |
| CAAAAACAAACAATACCGCA+GGG | - | chr2.4:56985228-56985247 | None:intergenic | 35.0% | |
| CACTTATCGAAGATAACTCA+AGG | + | chr2.4:56984408-56984427 | MS.gene058261:CDS | 35.0% | |
| CTTGAGTTATCTTCGATAAG+TGG | - | chr2.4:56984410-56984429 | None:intergenic | 35.0% | |
| GGATCAATATCACAACACTA+AGG | - | chr2.4:56985638-56985657 | None:intergenic | 35.0% | |
| GTTTCTTTGGCATTTCTTTC+TGG | + | chr2.4:56985163-56985182 | MS.gene058261:intron | 35.0% | |
| TATGATGAAAGTGGTGAACA+AGG | + | chr2.4:56984535-56984554 | MS.gene058261:CDS | 35.0% | |
| TATGCAATGGTCACAAAACA+AGG | - | chr2.4:56984940-56984959 | None:intergenic | 35.0% | |
| TTCATTGTCATAATCTGCCA+AGG | + | chr2.4:56984803-56984822 | MS.gene058261:CDS | 35.0% | |
| TTCCATCATAAGCTACACTT+TGG | - | chr2.4:56985315-56985334 | None:intergenic | 35.0% | |
| TTGTGCAATGTTTGGATTGA+CGG | + | chr2.4:56985245-56985264 | MS.gene058261:intron | 35.0% | |
| TTTGTTGGAGGTTCTGTTAA+TGG | + | chr2.4:56984455-56984474 | MS.gene058261:CDS | 35.0% | |
| ! | AATGGTTGAAATTTTGCGAG+GGG | + | chr2.4:56985702-56985721 | MS.gene058261:intron | 35.0% |
| ! | CTAATGGTTGAAATTTTGCG+AGG | + | chr2.4:56985700-56985719 | MS.gene058261:intron | 35.0% |
| ! | GGTTTGTCCAATTGTTTCTT+TGG | + | chr2.4:56985150-56985169 | MS.gene058261:intron | 35.0% |
| !! | AATCACTTGATGGATTGAGT+TGG | - | chr2.4:56985551-56985570 | None:intergenic | 35.0% |
| !! | ATTTCTAGTGGATTTGTTGG+AGG | + | chr2.4:56984443-56984462 | MS.gene058261:CDS | 35.0% |
| ACAAAACTGCAACCATCAAG+CGG | - | chr2.4:56985783-56985802 | None:intergenic | 40.0% | |
| AGAGTTTAGAGATATCACGG+TGG | + | chr2.4:56985268-56985287 | MS.gene058261:intron | 40.0% | |
| AGGCTCCAGCAAAAGAAAAA+CGG | + | chr2.4:56984555-56984574 | MS.gene058261:CDS | 40.0% | |
| AGTGAGTACACGAGAAATCA+TGG | + | chr2.4:56985036-56985055 | MS.gene058261:intron | 40.0% | |
| ATCTAATAGCAGCAGCATTG+TGG | + | chr2.4:56984757-56984776 | MS.gene058261:CDS | 40.0% | |
| ATGCTGCTGCTATTAGATGA+CGG | - | chr2.4:56984755-56984774 | None:intergenic | 40.0% | |
| CAGATTCGCAAGAAAAACTG+AGG | + | chr2.4:56985820-56985839 | MS.gene058261:CDS | 40.0% | |
| CGGAGAGTTTAGAGATATCA+CGG | + | chr2.4:56985265-56985284 | MS.gene058261:intron | 40.0% | |
| CTCCAAAGTGTAGCTTATGA+TGG | + | chr2.4:56985310-56985329 | MS.gene058261:intron | 40.0% | |
| GTGAGTACACGAGAAATCAT+GGG | + | chr2.4:56985037-56985056 | MS.gene058261:intron | 40.0% | |
| TACACGAGAAATCATGGGTT+TGG | + | chr2.4:56985042-56985061 | MS.gene058261:intron | 40.0% | |
| TTGGATTAAACTACTCCCTG+CGG | + | chr2.4:56985210-56985229 | MS.gene058261:intron | 40.0% | |
| TTTATGATCTGCTGCATCCA+AGG | - | chr2.4:56984636-56984655 | None:intergenic | 40.0% | |
| ! | AACAGAGGAGGGTTTGATTT+CGG | + | chr2.4:56985518-56985537 | MS.gene058261:CDS | 40.0% |
| ! | AATTTTGCGAGGGGATAAAG+AGG | + | chr2.4:56985711-56985730 | MS.gene058261:intron | 40.0% |
| ! | ACGTGAAATTTTCCGCTTGA+TGG | + | chr2.4:56985768-56985787 | MS.gene058261:intron | 40.0% |
| ! | AGAAAAACGGAAAAAGGCGT+CGG | + | chr2.4:56984568-56984587 | MS.gene058261:CDS | 40.0% |
| ! | AGCTTATGATGGAATCACGA+TGG | + | chr2.4:56985321-56985340 | MS.gene058261:intron | 40.0% |
| ! | GAGAAATCATGGGTTTGGTT+TGG | + | chr2.4:56985047-56985066 | MS.gene058261:intron | 40.0% |
| ! | TATCTCGTAGTGGGATTCAA+AGG | - | chr2.4:56985659-56985678 | None:intergenic | 40.0% |
| !! | ATGGTTGCAGTTTTGTACAG+CGG | + | chr2.4:56985787-56985806 | MS.gene058261:intron | 40.0% |
| AGCTCAGTGTCAAGTTTCCA+AGG | + | chr2.4:56985474-56985493 | MS.gene058261:CDS | 45.0% | |
| CAAGTGATTCAAATGCGCCA+GGG | + | chr2.4:56985562-56985581 | MS.gene058261:CDS | 45.0% | |
| GGCGCATTTGAATCACTTGA+TGG | - | chr2.4:56985561-56985580 | None:intergenic | 45.0% | |
| TCAAGTGATTCAAATGCGCC+AGG | + | chr2.4:56985561-56985580 | MS.gene058261:CDS | 45.0% | |
| TCGCAAGAAAAACTGAGGAC+AGG | + | chr2.4:56985825-56985844 | MS.gene058261:CDS | 45.0% | |
| TTTGCAGGCATGTCATCAAC+TGG | + | chr2.4:56985447-56985466 | MS.gene058261:intron | 45.0% | |
| ! | GCGAAGTGTTATCTCGTAGT+GGG | - | chr2.4:56985668-56985687 | None:intergenic | 45.0% |
| !! | GAAAAACGGAAAAAGGCGTC+GGG | + | chr2.4:56984569-56984588 | MS.gene058261:CDS | 45.0% |
| !! | GAAAAAGGCGTCGGGTAAAA+AGG | + | chr2.4:56984577-56984596 | MS.gene058261:CDS | 45.0% |
| !! | GTTGATGACATGCCTGCAAA+AGG | - | chr2.4:56985447-56985466 | None:intergenic | 45.0% |
| !! | TGGTTGCAGTTTTGTACAGC+GGG | + | chr2.4:56985788-56985807 | MS.gene058261:intron | 45.0% |
| !!! | ATAAATATGAAAATTTTATA+TGG | - | chr2.4:56984986-56985005 | None:intergenic | 5.0% |
| CAAGGATCACCTGTATCAGC+AGG | + | chr2.4:56985492-56985511 | MS.gene058261:CDS | 50.0% | |
| CTGCTGATACAGGTGATCCT+TGG | - | chr2.4:56985494-56985513 | None:intergenic | 50.0% | |
| GTTCTGTTAATGGCGAAGCC+AGG | + | chr2.4:56984465-56984484 | MS.gene058261:CDS | 50.0% | |
| TCTGTTCCTCCTGCTGATAC+AGG | - | chr2.4:56985504-56985523 | None:intergenic | 50.0% | |
| TGATCTGCTGCATCCAAGGA+CGG | - | chr2.4:56984632-56984651 | None:intergenic | 50.0% | |
| TGATTCAAATGCGCCAGGGT+CGG | + | chr2.4:56985566-56985585 | MS.gene058261:CDS | 50.0% | |
| TGTATCAGCAGGAGGAACAG+AGG | + | chr2.4:56985503-56985522 | MS.gene058261:CDS | 50.0% | |
| TTGCGATGTTGAAGCGTGCA+AGG | + | chr2.4:56984678-56984697 | MS.gene058261:CDS | 50.0% | |
| ! | GGCGAAGTGTTATCTCGTAG+TGG | - | chr2.4:56985669-56985688 | None:intergenic | 50.0% |
| ! | GTTTGGAATGGCGGTGAGTT+TGG | - | chr2.4:56985364-56985383 | None:intergenic | 50.0% |
| !! | AGTTTTGTACAGCGGGTGTC+AGG | + | chr2.4:56985795-56985814 | MS.gene058261:intron | 50.0% |
| ATCAGCAGGAGGAACAGAGG+AGG | + | chr2.4:56985506-56985525 | MS.gene058261:CDS | 55.0% | |
| GATCTGCTGCATCCAAGGAC+GGG | - | chr2.4:56984631-56984650 | None:intergenic | 55.0% | |
| GGATCACCTGTATCAGCAGG+AGG | + | chr2.4:56985495-56985514 | MS.gene058261:CDS | 55.0% | |
| TCAGCAGGAGGAACAGAGGA+GGG | + | chr2.4:56985507-56985526 | MS.gene058261:CDS | 55.0% | |
| !! | TACTTGATGGACTCCGACCC+TGG | - | chr2.4:56985582-56985601 | None:intergenic | 55.0% |
| AAACCTCCGCGACCTCCACA+AGG | + | chr2.4:56984605-56984624 | MS.gene058261:CDS | 60.0% | |
| TTGTGGAGGTCGCGGAGGTT+TGG | - | chr2.4:56984606-56984625 | None:intergenic | 60.0% | |
| ACCTCCACAAGGCCCGTCCT+TGG | + | chr2.4:56984616-56984635 | MS.gene058261:CDS | 65.0% | |
| GCATCCAAGGACGGGCCTTG+TGG | - | chr2.4:56984623-56984642 | None:intergenic | 65.0% | |
| ! | TCCAAGGACGGGCCTTGTGG+AGG | - | chr2.4:56984620-56984639 | None:intergenic | 65.0% |
| ! | GACGGGCCTTGTGGAGGTCG+CGG | - | chr2.4:56984614-56984633 | None:intergenic | 70.0% |
| ! | GGGCCTTGTGGAGGTCGCGG+AGG | - | chr2.4:56984611-56984630 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 56984350 | 56985851 | 56984350 | ID=MS.gene058261 |
| chr2.4 | mRNA | 56984350 | 56985851 | 56984350 | ID=MS.gene058261.t1;Parent=MS.gene058261 |
| chr2.4 | exon | 56984350 | 56984824 | 56984350 | ID=MS.gene058261.t1.exon1;Parent=MS.gene058261.t1 |
| chr2.4 | CDS | 56984350 | 56984824 | 56984350 | ID=cds.MS.gene058261.t1;Parent=MS.gene058261.t1 |
| chr2.4 | exon | 56985454 | 56985598 | 56985454 | ID=MS.gene058261.t1.exon2;Parent=MS.gene058261.t1 |
| chr2.4 | CDS | 56985454 | 56985598 | 56985454 | ID=cds.MS.gene058261.t1;Parent=MS.gene058261.t1 |
| chr2.4 | exon | 56985797 | 56985851 | 56985797 | ID=MS.gene058261.t1.exon3;Parent=MS.gene058261.t1 |
| chr2.4 | CDS | 56985797 | 56985851 | 56985797 | ID=cds.MS.gene058261.t1;Parent=MS.gene058261.t1 |
| Gene Sequence |
| Protein sequence |
