Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32574.t1 | XP_024640395.1 | 98 | 50 | 1 | 0 | 144 | 193 | 267 | 316 | 2.10E-19 | 105.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32574.t1 | G7KBP4 | 98.0 | 50 | 1 | 0 | 144 | 193 | 267 | 316 | 1.5e-19 | 105.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene32574 | 0.801182 | 1.02E-48 | -1.69E-46 |
| MS.gene049825 | MS.gene32574 | 0.807226 | 5.61E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene32574 | 0.802202 | 6.27E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene32574 | 0.80731 | 5.38E-50 | -1.69E-46 |
| MS.gene05548 | MS.gene32574 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene055512 | MS.gene32574 | 0.800545 | 1.37E-48 | -1.69E-46 |
| MS.gene057057 | MS.gene32574 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene32574 | 0.803445 | 3.47E-49 | -1.69E-46 |
| MS.gene057555 | MS.gene32574 | 0.812915 | 3.33E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene32574 | 0.840993 | 6.22E-58 | -1.69E-46 |
| MS.gene059317 | MS.gene32574 | 0.815004 | 1.15E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene32574 | 0.813912 | 2.01E-51 | -1.69E-46 |
| MS.gene060619 | MS.gene32574 | 0.80606 | 9.88E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 64 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCATCATGGTGGTGGTTT+GGG | 0.171912 | 5.1:-61951915 | MS.gene32574:CDS |
| GGGATGGGTGGTGCTGGATT+AGG | 0.238214 | 5.1:-61951957 | MS.gene32574:CDS |
| AATTGAAGAGATTAGGAGTT+AGG | 0.242633 | 5.1:-61952388 | MS.gene32574:CDS |
| GTTGCATCATGGTGGTGGTT+TGG | 0.285567 | 5.1:-61951916 | MS.gene32574:CDS |
| AGGAGGTTGAGAAGAGAGTT+AGG | 0.287828 | 5.1:-61952196 | MS.gene32574:CDS |
| AATTGGAATTGGAGGCGATA+TGG | 0.299496 | 5.1:-61952226 | MS.gene32574:CDS |
| AATGATGGGATGGGTGGTGC+TGG | 0.316086 | 5.1:-61951963 | MS.gene32574:CDS |
| GAGAAGAGAGGAAGAGGGAG+AGG | 0.326670 | 5.1:-61952334 | MS.gene32574:CDS |
| TGGGGAGAGGGAATTGGAAT+TGG | 0.332648 | 5.1:-61952237 | MS.gene32574:CDS |
| TTTGAAATTGAGGGAGGTTA+TGG | 0.333645 | 5.1:-61952363 | MS.gene32574:CDS |
| GGATGGGTGGTGCTGGATTA+GGG | 0.339968 | 5.1:-61951956 | MS.gene32574:CDS |
| GAGGAAGGTGGAAGAGAAGA+TGG | 0.360691 | 5.1:-61952138 | MS.gene32574:CDS |
| AGGAGAAAGAGGAGTGGAAA+AGG | 0.372350 | 5.1:-61952301 | MS.gene32574:CDS |
| AATGCAGGTTCTTGGTGTTA+TGG | 0.373631 | 5.1:-61952018 | MS.gene32574:CDS |
| ATTGAAGAGATTAGGAGTTA+GGG | 0.381286 | 5.1:-61952387 | MS.gene32574:CDS |
| GATGGAGTGGAGGGAGAAGA+TGG | 0.388023 | 5.1:-61952105 | MS.gene32574:CDS |
| CATAATCAAATGCAGGTTCT+TGG | 0.399935 | 5.1:-61952026 | MS.gene32574:CDS |
| GGAAGAGAAGATGGAGGAAG+AGG | 0.411440 | 5.1:-61952129 | MS.gene32574:CDS |
| GAAGAGGAGGAGGAGGAAGG+TGG | 0.415213 | 5.1:-61952150 | MS.gene32574:CDS |
| GAAGATGGAGGAAGAGGAGA+TGG | 0.423990 | 5.1:-61952123 | MS.gene32574:CDS |
| GGAGAAGAGGAGGAGGAGGA+AGG | 0.434983 | 5.1:-61952153 | MS.gene32574:CDS |
| TTGTTGTGGGGAGAGGGAAT+TGG | 0.442224 | 5.1:-61952243 | MS.gene32574:CDS |
| TGAAGAGAGAAGAGAGGAAG+AGG | 0.442759 | 5.1:-61952340 | MS.gene32574:CDS |
| TGGTTCGGGGAATGATGGGA+TGG | 0.445450 | 5.1:-61951973 | MS.gene32574:CDS |
| GCGAGTGTTGTTGTGGGGAG+AGG | 0.445649 | 5.1:-61952250 | MS.gene32574:CDS |
| GAAGAAGAATTGAAGAGATT+AGG | 0.446508 | 5.1:-61952395 | MS.gene32574:CDS |
| GGAAGAGGAGATGGAGTGGA+GGG | 0.450080 | 5.1:-61952114 | MS.gene32574:CDS |
| AGGAGGAGAAGAGGAGGAGG+AGG | 0.451794 | 5.1:-61952157 | MS.gene32574:CDS |
| AGGAGCTTGAGGAGGAGAAG+AGG | 0.455597 | 5.1:-61952166 | MS.gene32574:CDS |
| ATTGGAATTGGAGGCGATAT+GGG | 0.456138 | 5.1:-61952225 | MS.gene32574:CDS |
| AGGAAGAGGAGATGGAGTGG+AGG | 0.468289 | 5.1:-61952115 | MS.gene32574:CDS |
| TTGAGGAGGAGAAGAGGAGG+AGG | 0.474734 | 5.1:-61952160 | MS.gene32574:CDS |
| GAAGAGAGTTAGGTTAGAGA+AGG | 0.486337 | 5.1:-61952186 | MS.gene32574:CDS |
| TGGAGGAAGAGGAGATGGAG+TGG | 0.487474 | 5.1:-61952118 | MS.gene32574:CDS |
| TGCATCATGGTGGTGGTTTG+GGG | 0.496478 | 5.1:-61951914 | MS.gene32574:CDS |
| TCGGGGAATGATGGGATGGG+TGG | 0.500258 | 5.1:-61951969 | MS.gene32574:CDS |
| CGAGTGTTGTTGTGGGGAGA+GGG | 0.501701 | 5.1:-61952249 | MS.gene32574:CDS |
| AGAAGAGAGGAAGAGGGAGA+GGG | 0.503467 | 5.1:-61952333 | MS.gene32574:CDS |
| GACGACTCTGATGATTCTAA+TGG | 0.504562 | 5.1:-61952452 | MS.gene32574:CDS |
| GGTTCGGGGAATGATGGGAT+GGG | 0.514502 | 5.1:-61951972 | MS.gene32574:CDS |
| GAAGAGAGAAGAGAGGAAGA+GGG | 0.514578 | 5.1:-61952339 | MS.gene32574:CDS |
| AGCTTGAGGAGGAGAAGAGG+AGG | 0.514591 | 5.1:-61952163 | MS.gene32574:CDS |
| GGAGAGGGAATTGGAATTGG+AGG | 0.516815 | 5.1:-61952234 | MS.gene32574:CDS |
| CAGGTGTTGCATCATGGTGG+TGG | 0.520118 | 5.1:-61951921 | MS.gene32574:CDS |
| GGAGGCGATATGGGCGAAGA+AGG | 0.523248 | 5.1:-61952216 | MS.gene32574:CDS |
| TTATGGTGAAGAGAGAAGAG+AGG | 0.532365 | 5.1:-61952346 | MS.gene32574:CDS |
| GAAAGAGGAGTGGAAAAGGA+AGG | 0.534415 | 5.1:-61952297 | MS.gene32574:CDS |
| GGAAAAGGCGAGTGTTGTTG+TGG | 0.534960 | 5.1:-61952257 | MS.gene32574:CDS |
| GTTAGAGAAGGAGCTTGAGG+AGG | 0.535819 | 5.1:-61952174 | MS.gene32574:CDS |
| ATTAGGGACATTGCCGACTC+AGG | 0.541810 | 5.1:-61951940 | MS.gene32574:CDS |
| TAGGTTAGAGAAGGAGCTTG+AGG | 0.544840 | 5.1:-61952177 | MS.gene32574:CDS |
| GAAAAGGCGAGTGTTGTTGT+GGG | 0.546917 | 5.1:-61952256 | MS.gene32574:CDS |
| GAAGGTGGAAGAGAAGATGG+AGG | 0.560726 | 5.1:-61952135 | MS.gene32574:CDS |
| GATGAGGACGATGATGGTGA+TGG | 0.567119 | 5.1:-61952428 | MS.gene32574:CDS |
| CCATGATGCAACACCTGAGT+CGG | 0.586713 | 5.1:+61951927 | None:intergenic |
| CCGACTCAGGTGTTGCATCA+TGG | 0.587073 | 5.1:-61951927 | MS.gene32574:CDS |
| GGAGTGGAGGGAGAAGATGG+TGG | 0.591696 | 5.1:-61952102 | MS.gene32574:CDS |
| GGCGTGTCATAATCAAATGC+AGG | 0.593011 | 5.1:-61952033 | MS.gene32574:CDS |
| GGCGATATGGGCGAAGAAGG+AGG | 0.625755 | 5.1:-61952213 | MS.gene32574:CDS |
| AATGGTGATGAGGACGATGA+TGG | 0.634800 | 5.1:-61952434 | MS.gene32574:CDS |
| TGATGATTCTAATGGTGATG+AGG | 0.634936 | 5.1:-61952444 | MS.gene32574:CDS |
| AAAAGGCGAGTGTTGTTGTG+GGG | 0.698921 | 5.1:-61952255 | MS.gene32574:CDS |
| ACTCAGGTGTTGCATCATGG+TGG | 0.707815 | 5.1:-61951924 | MS.gene32574:CDS |
| GATGATGCAGATGCATGCTG+AGG | 0.710436 | 5.1:-61952054 | MS.gene32574:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTGAAGAGATTAGGAGTT+AGG | - | chr5.1:61951992-61952011 | MS.gene32574:CDS | 30.0% | |
| ATTGAAGAGATTAGGAGTTA+GGG | - | chr5.1:61951993-61952012 | MS.gene32574:CDS | 30.0% | |
| GAAGAAGAATTGAAGAGATT+AGG | - | chr5.1:61951985-61952004 | MS.gene32574:CDS | 30.0% | |
| !!! | AGAATACTGTGTCAGTTTTT+TGG | - | chr5.1:61952387-61952406 | MS.gene32574:CDS | 30.0% |
| !!! | AGTTAGGGTTTTGAAATTGA+GGG | - | chr5.1:61952008-61952027 | MS.gene32574:CDS | 30.0% |
| CATAATCAAATGCAGGTTCT+TGG | - | chr5.1:61952354-61952373 | MS.gene32574:CDS | 35.0% | |
| ! | TGATGATTCTAATGGTGATG+AGG | - | chr5.1:61951936-61951955 | MS.gene32574:CDS | 35.0% |
| ! | TTTGAAATTGAGGGAGGTTA+TGG | - | chr5.1:61952017-61952036 | MS.gene32574:CDS | 35.0% |
| !! | AAGGAATTTGAGTTTTCGAG+TGG | - | chr5.1:61952102-61952121 | MS.gene32574:CDS | 35.0% |
| !!! | ACTGTGTCAGTTTTTTGGTT+CGG | - | chr5.1:61952392-61952411 | MS.gene32574:CDS | 35.0% |
| !!! | GAGTTAGGGTTTTGAAATTG+AGG | - | chr5.1:61952007-61952026 | MS.gene32574:CDS | 35.0% |
| !!! | TTTGAGTTTTCGAGTGGAAA+AGG | - | chr5.1:61952108-61952127 | MS.gene32574:CDS | 35.0% |
| AATTGGAATTGGAGGCGATA+TGG | - | chr5.1:61952154-61952173 | MS.gene32574:CDS | 40.0% | |
| ATTGGAATTGGAGGCGATAT+GGG | - | chr5.1:61952155-61952174 | MS.gene32574:CDS | 40.0% | |
| GAAGAGAGTTAGGTTAGAGA+AGG | - | chr5.1:61952194-61952213 | MS.gene32574:CDS | 40.0% | |
| GACGACTCTGATGATTCTAA+TGG | - | chr5.1:61951928-61951947 | MS.gene32574:CDS | 40.0% | |
| TTATGGTGAAGAGAGAAGAG+AGG | - | chr5.1:61952034-61952053 | MS.gene32574:CDS | 40.0% | |
| !! | AATGCAGGTTCTTGGTGTTA+TGG | - | chr5.1:61952362-61952381 | MS.gene32574:CDS | 40.0% |
| !! | GGAGTTTTTGAAGGAGAAAG+AGG | - | chr5.1:61952068-61952087 | MS.gene32574:CDS | 40.0% |
| !! | TTTTGAAGGAGAAAGAGGAG+TGG | - | chr5.1:61952073-61952092 | MS.gene32574:CDS | 40.0% |
| !!! | CTGTGTCAGTTTTTTGGTTC+GGG | - | chr5.1:61952393-61952412 | MS.gene32574:CDS | 40.0% |
| !!! | TAGGGTTTTGAAATTGAGGG+AGG | - | chr5.1:61952011-61952030 | MS.gene32574:CDS | 40.0% |
| !!! | TGTGTCAGTTTTTTGGTTCG+GGG | - | chr5.1:61952394-61952413 | MS.gene32574:CDS | 40.0% |
| !!! | TTTTTGGTTCGGGGAATGAT+GGG | - | chr5.1:61952403-61952422 | MS.gene32574:CDS | 40.0% |
| !!! | TTTTTTGGTTCGGGGAATGA+TGG | - | chr5.1:61952402-61952421 | MS.gene32574:CDS | 40.0% |
| AGGAGAAAGAGGAGTGGAAA+AGG | - | chr5.1:61952079-61952098 | MS.gene32574:CDS | 45.0% | |
| AGGAGGTTGAGAAGAGAGTT+AGG | - | chr5.1:61952184-61952203 | MS.gene32574:CDS | 45.0% | |
| GAAAGAGGAGTGGAAAAGGA+AGG | - | chr5.1:61952083-61952102 | MS.gene32574:CDS | 45.0% | |
| GAAGAGAGAAGAGAGGAAGA+GGG | - | chr5.1:61952041-61952060 | MS.gene32574:CDS | 45.0% | |
| GGCGTGTCATAATCAAATGC+AGG | - | chr5.1:61952347-61952366 | MS.gene32574:CDS | 45.0% | |
| TAGGTTAGAGAAGGAGCTTG+AGG | - | chr5.1:61952203-61952222 | MS.gene32574:CDS | 45.0% | |
| TGAAGAGAGAAGAGAGGAAG+AGG | - | chr5.1:61952040-61952059 | MS.gene32574:CDS | 45.0% | |
| ! | AAAAGGCGAGTGTTGTTGTG+GGG | - | chr5.1:61952125-61952144 | MS.gene32574:CDS | 45.0% |
| ! | AATGGTGATGAGGACGATGA+TGG | - | chr5.1:61951946-61951965 | MS.gene32574:CDS | 45.0% |
| ! | GAAAAGGCGAGTGTTGTTGT+GGG | - | chr5.1:61952124-61952143 | MS.gene32574:CDS | 45.0% |
| !! | TTGCATCATGGTGGTGGTTT+GGG | - | chr5.1:61952465-61952484 | MS.gene32574:CDS | 45.0% |
| AGAAGAGAGGAAGAGGGAGA+GGG | - | chr5.1:61952047-61952066 | MS.gene32574:CDS | 50.0% | |
| CCATGATGCAACACCTGAGT+CGG | + | chr5.1:61952456-61952475 | None:intergenic | 50.0% | |
| GAAGATGGAGGAAGAGGAGA+TGG | - | chr5.1:61952257-61952276 | MS.gene32574:CDS | 50.0% | |
| GAAGGTGGAAGAGAAGATGG+AGG | - | chr5.1:61952245-61952264 | MS.gene32574:CDS | 50.0% | |
| GAGGAAGGTGGAAGAGAAGA+TGG | - | chr5.1:61952242-61952261 | MS.gene32574:CDS | 50.0% | |
| GATGATGCAGATGCATGCTG+AGG | - | chr5.1:61952326-61952345 | MS.gene32574:CDS | 50.0% | |
| GGAAGAGAAGATGGAGGAAG+AGG | - | chr5.1:61952251-61952270 | MS.gene32574:CDS | 50.0% | |
| GGAGAGGGAATTGGAATTGG+AGG | - | chr5.1:61952146-61952165 | MS.gene32574:CDS | 50.0% | |
| GTTAGAGAAGGAGCTTGAGG+AGG | - | chr5.1:61952206-61952225 | MS.gene32574:CDS | 50.0% | |
| TGGGGAGAGGGAATTGGAAT+TGG | - | chr5.1:61952143-61952162 | MS.gene32574:CDS | 50.0% | |
| TTGTTGTGGGGAGAGGGAAT+TGG | - | chr5.1:61952137-61952156 | MS.gene32574:CDS | 50.0% | |
| ! | ACTCAGGTGTTGCATCATGG+TGG | - | chr5.1:61952456-61952475 | MS.gene32574:CDS | 50.0% |
| ! | GAGGGAGAGGGAGTTTTTGA+AGG | - | chr5.1:61952059-61952078 | MS.gene32574:CDS | 50.0% |
| ! | GATGAGGACGATGATGGTGA+TGG | - | chr5.1:61951952-61951971 | MS.gene32574:CDS | 50.0% |
| ! | GGAAAAGGCGAGTGTTGTTG+TGG | - | chr5.1:61952123-61952142 | MS.gene32574:CDS | 50.0% |
| !! | ATTAGGGACATTGCCGACTC+AGG | - | chr5.1:61952440-61952459 | MS.gene32574:CDS | 50.0% |
| !! | GTTGCATCATGGTGGTGGTT+TGG | - | chr5.1:61952464-61952483 | MS.gene32574:CDS | 50.0% |
| !! | TGCATCATGGTGGTGGTTTG+GGG | - | chr5.1:61952466-61952485 | MS.gene32574:CDS | 50.0% |
| AGCTTGAGGAGGAGAAGAGG+AGG | - | chr5.1:61952217-61952236 | MS.gene32574:CDS | 55.0% | |
| AGGAAGAGGAGATGGAGTGG+AGG | - | chr5.1:61952265-61952284 | MS.gene32574:CDS | 55.0% | |
| AGGAGCTTGAGGAGGAGAAG+AGG | - | chr5.1:61952214-61952233 | MS.gene32574:CDS | 55.0% | |
| GAGAAGAGAGGAAGAGGGAG+AGG | - | chr5.1:61952046-61952065 | MS.gene32574:CDS | 55.0% | |
| GATGGAGTGGAGGGAGAAGA+TGG | - | chr5.1:61952275-61952294 | MS.gene32574:CDS | 55.0% | |
| GGAAGAGGAGATGGAGTGGA+GGG | - | chr5.1:61952266-61952285 | MS.gene32574:CDS | 55.0% | |
| TGGAGGAAGAGGAGATGGAG+TGG | - | chr5.1:61952262-61952281 | MS.gene32574:CDS | 55.0% | |
| TTGAGGAGGAGAAGAGGAGG+AGG | - | chr5.1:61952220-61952239 | MS.gene32574:CDS | 55.0% | |
| ! | AATGATGGGATGGGTGGTGC+TGG | - | chr5.1:61952417-61952436 | MS.gene32574:CDS | 55.0% |
| ! | CAGGTGTTGCATCATGGTGG+TGG | - | chr5.1:61952459-61952478 | MS.gene32574:CDS | 55.0% |
| ! | CCGACTCAGGTGTTGCATCA+TGG | - | chr5.1:61952453-61952472 | MS.gene32574:CDS | 55.0% |
| ! | CGAGTGTTGTTGTGGGGAGA+GGG | - | chr5.1:61952131-61952150 | MS.gene32574:CDS | 55.0% |
| ! | GGATGGGTGGTGCTGGATTA+GGG | - | chr5.1:61952424-61952443 | MS.gene32574:CDS | 55.0% |
| ! | GGTTCGGGGAATGATGGGAT+GGG | - | chr5.1:61952408-61952427 | MS.gene32574:CDS | 55.0% |
| ! | TGGTTCGGGGAATGATGGGA+TGG | - | chr5.1:61952407-61952426 | MS.gene32574:CDS | 55.0% |
| !! | CATGGTGGTGGTTTGGGGAA+TGG | - | chr5.1:61952471-61952490 | MS.gene32574:CDS | 55.0% |
| AGGAGGAGAAGAGGAGGAGG+AGG | - | chr5.1:61952223-61952242 | MS.gene32574:CDS | 60.0% | |
| GAAGAGGAGGAGGAGGAAGG+TGG | - | chr5.1:61952230-61952249 | MS.gene32574:CDS | 60.0% | |
| GGAGAAGAGGAGGAGGAGGA+AGG | - | chr5.1:61952227-61952246 | MS.gene32574:CDS | 60.0% | |
| GGAGGCGATATGGGCGAAGA+AGG | - | chr5.1:61952164-61952183 | MS.gene32574:CDS | 60.0% | |
| GGAGTGGAGGGAGAAGATGG+TGG | - | chr5.1:61952278-61952297 | MS.gene32574:CDS | 60.0% | |
| GGCGATATGGGCGAAGAAGG+AGG | - | chr5.1:61952167-61952186 | MS.gene32574:CDS | 60.0% | |
| ! | GCGAGTGTTGTTGTGGGGAG+AGG | - | chr5.1:61952130-61952149 | MS.gene32574:CDS | 60.0% |
| ! | GGGATGGGTGGTGCTGGATT+AGG | - | chr5.1:61952423-61952442 | MS.gene32574:CDS | 60.0% |
| ! | TCGGGGAATGATGGGATGGG+TGG | - | chr5.1:61952411-61952430 | MS.gene32574:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 61951911 | 61952491 | 61951911 | ID=MS.gene32574 |
| chr5.1 | mRNA | 61951911 | 61952491 | 61951911 | ID=MS.gene32574.t1;Parent=MS.gene32574 |
| chr5.1 | exon | 61951911 | 61952491 | 61951911 | ID=MS.gene32574.t1.exon1;Parent=MS.gene32574.t1 |
| chr5.1 | CDS | 61951911 | 61952491 | 61951911 | ID=cds.MS.gene32574.t1;Parent=MS.gene32574.t1 |
| Gene Sequence |
| Protein sequence |
