Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32861.t1 | XP_013461803.1 | 99 | 394 | 4 | 0 | 1 | 394 | 1 | 394 | 4.80E-220 | 773.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32861.t1 | Q801S4 | 28.4 | 292 | 151 | 13 | 44 | 323 | 31 | 276 | 5.0e-16 | 87.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32861.t1 | A0A072V3C4 | 99.0 | 394 | 4 | 0 | 1 | 394 | 1 | 394 | 3.5e-220 | 773.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene32861 | 0.806049 | 9.93E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene32861 | 0.802427 | 5.64E-49 | -1.69E-46 |
| MS.gene051201 | MS.gene32861 | 0.800608 | 1.33E-48 | -1.69E-46 |
| MS.gene051279 | MS.gene32861 | 0.810561 | 1.08E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene32861 | 0.801468 | 8.88E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene32861 | 0.829724 | 4.39E-55 | -1.69E-46 |
| MS.gene055229 | MS.gene32861 | 0.802739 | 4.86E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene32861 | 0.811477 | 6.87E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene32861 | 0.808283 | 3.34E-50 | -1.69E-46 |
| MS.gene060348 | MS.gene32861 | 0.806475 | 8.08E-50 | -1.69E-46 |
| MS.gene060352 | MS.gene32861 | 0.801981 | 6.97E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32861.t1 | MTR_3g103350 | 98.985 | 394 | 4 | 0 | 1 | 394 | 1 | 394 | 0.0 | 806 |
| MS.gene32861.t1 | MTR_3g103350 | 98.710 | 310 | 4 | 0 | 1 | 310 | 1 | 310 | 0.0 | 633 |
| MS.gene32861.t1 | MTR_3g103350 | 98.148 | 270 | 5 | 0 | 1 | 270 | 1 | 270 | 0.0 | 549 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32861.t1 | AT1G59520 | 68.020 | 394 | 119 | 2 | 1 | 394 | 1 | 387 | 0.0 | 522 |
| MS.gene32861.t1 | AT1G59520 | 67.513 | 394 | 121 | 2 | 1 | 394 | 1 | 387 | 0.0 | 519 |
| MS.gene32861.t1 | AT1G59520 | 67.959 | 387 | 117 | 2 | 1 | 387 | 1 | 380 | 0.0 | 511 |
| MS.gene32861.t1 | AT1G59520 | 61.675 | 394 | 109 | 2 | 1 | 394 | 1 | 352 | 1.11e-173 | 488 |
| MS.gene32861.t1 | AT1G59520 | 68.932 | 309 | 90 | 1 | 1 | 309 | 1 | 303 | 7.75e-159 | 449 |
| MS.gene32861.t1 | AT1G59520 | 68.932 | 309 | 90 | 1 | 1 | 309 | 1 | 303 | 7.75e-159 | 449 |
Find 81 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAATGGATATGAAATTT+TGG | 0.207384 | 3.1:+81835988 | MS.gene32861:CDS |
| CTACATTTACCTTATATATA+GGG | 0.220686 | 3.1:-81837859 | None:intergenic |
| TCTCCAGTTGTATCAAATAA+AGG | 0.233028 | 3.1:+81843405 | MS.gene32861:CDS |
| ACTACATTTACCTTATATAT+AGG | 0.247282 | 3.1:-81837860 | None:intergenic |
| AGCGTATGATCTTCTGTTTA+AGG | 0.247512 | 3.1:+81843584 | MS.gene32861:CDS |
| AAGAAGCACTCAAACTTAAT+TGG | 0.259747 | 3.1:+81835930 | MS.gene32861:CDS |
| GAAGTTGAAGTAGCTGTTTC+TGG | 0.265731 | 3.1:+81842415 | MS.gene32861:CDS |
| GCCCACCTGCGTACAAAATA+AGG | 0.297730 | 3.1:-81836520 | None:intergenic |
| ATCTTTATCCCCTATATATA+AGG | 0.309094 | 3.1:+81837850 | MS.gene32861:CDS |
| GAAAATGGCCTACTGATAAA+AGG | 0.315754 | 3.1:-81842345 | None:intergenic |
| CCCTCAGTTGAGCAACTTAG+TGG | 0.325676 | 3.1:+81837172 | MS.gene32861:intron |
| GCGCAGGTTTCTCGAGGTTC+TGG | 0.328303 | 3.1:+81836469 | MS.gene32861:intron |
| GATCAAAGCCAGCAGGATTC+AGG | 0.333189 | 3.1:+81843375 | MS.gene32861:intron |
| AACCCTTTATTTGATACAAC+TGG | 0.334616 | 3.1:-81843408 | None:intergenic |
| GTAGCTGTTTCTGGTGTTGC+AGG | 0.340437 | 3.1:+81842424 | MS.gene32861:CDS |
| GCAGCTGGAAAATCTCGATT+TGG | 0.343845 | 3.1:+81842319 | MS.gene32861:intron |
| TAGATATGAATTGTTGAGTA+TGG | 0.351632 | 3.1:+81835905 | MS.gene32861:intron |
| TTCAACTTCCCCACGGCCTC+CGG | 0.358922 | 3.1:-81842401 | None:intergenic |
| TTCATATCCATTTCAACATC+AGG | 0.362331 | 3.1:-81835981 | None:intergenic |
| CTCCAGTTGTATCAAATAAA+GGG | 0.364902 | 3.1:+81843406 | MS.gene32861:CDS |
| ATGTATCCCTCCCAGCTTGA+TGG | 0.384464 | 3.1:-81837829 | None:intergenic |
| GTTGTATCAAATAAAGGGTT+CGG | 0.390728 | 3.1:+81843411 | MS.gene32861:CDS |
| ATATTCCCAAGGCAGCGATA+TGG | 0.401755 | 3.1:-81843559 | None:intergenic |
| TATATCTTTCTCTAGTCAAC+AGG | 0.403251 | 3.1:+81837796 | MS.gene32861:intron |
| AGCTGACAAATCCTGAGAAA+AGG | 0.409057 | 3.1:-81841525 | None:intergenic |
| ATGAAAGGTCCCGGAGGCCG+TGG | 0.416656 | 3.1:+81842391 | MS.gene32861:CDS |
| TTGTATCAAATAAAGGGTTC+GGG | 0.422955 | 3.1:+81843412 | MS.gene32861:CDS |
| CAGCTGGAAAATCTCGATTT+GGG | 0.430720 | 3.1:+81842320 | MS.gene32861:intron |
| TCAAATAAAGGGTTCGGGCT+TGG | 0.433966 | 3.1:+81843417 | MS.gene32861:CDS |
| GGAAACAACACCTGCTTATC+CGG | 0.439105 | 3.1:+81839554 | MS.gene32861:CDS |
| AAATAAGGATCAACACTCTC+TGG | 0.443748 | 3.1:-81836505 | None:intergenic |
| TCAACTTCCCCACGGCCTCC+GGG | 0.450302 | 3.1:-81842400 | None:intergenic |
| TAATGTCCATATCGCTGCCT+TGG | 0.454899 | 3.1:+81843553 | MS.gene32861:CDS |
| AGATGCTCCTGATGTTGAAA+TGG | 0.468987 | 3.1:+81835974 | MS.gene32861:CDS |
| TCAATTGCAAAACATATATC+CGG | 0.475756 | 3.1:-81839573 | None:intergenic |
| AAAATTTGAGCTAGAGGAAG+AGG | 0.477255 | 3.1:-81843502 | None:intergenic |
| GTTGTTCTTAATGCACATGA+TGG | 0.482727 | 3.1:+81840586 | MS.gene32861:CDS |
| AAACTTCAATAGACGTGGAT+TGG | 0.483473 | 3.1:+81837198 | MS.gene32861:CDS |
| GCTGACAAATCCTGAGAAAA+GGG | 0.483603 | 3.1:-81841524 | None:intergenic |
| GTAGAACTAGTACAAACTTC+CGG | 0.489726 | 3.1:-81840667 | None:intergenic |
| AATTGAAAATTGACACGACT+TGG | 0.495981 | 3.1:-81839404 | None:intergenic |
| GAATAATGAATGTACCTTGA+TGG | 0.498369 | 3.1:-81835800 | None:intergenic |
| AATGTCCATATCGCTGCCTT+GGG | 0.498979 | 3.1:+81843554 | MS.gene32861:CDS |
| TTCATCAAAATTTGAGCTAG+AGG | 0.499203 | 3.1:-81843508 | None:intergenic |
| AATAATGAATGTACCTTGAT+GGG | 0.499604 | 3.1:-81835799 | None:intergenic |
| TTCTTCAGAACCATCAAGCT+GGG | 0.515455 | 3.1:+81837819 | MS.gene32861:CDS |
| AGCATCTTGCTCTTCAACAA+CGG | 0.519180 | 3.1:-81835958 | None:intergenic |
| TTGTTCTTAATGCACATGAT+GGG | 0.519594 | 3.1:+81840587 | MS.gene32861:CDS |
| CTGTAACTGTTGCAATCTGC+AGG | 0.521700 | 3.1:+81837264 | MS.gene32861:CDS |
| AAACATATATCCGGATAAGC+AGG | 0.524022 | 3.1:-81839564 | None:intergenic |
| GGCAGCATTTCCTAGTGATA+AGG | 0.526186 | 3.1:+81840609 | MS.gene32861:CDS |
| CTTCAATAGACGTGGATTGG+AGG | 0.526656 | 3.1:+81837201 | MS.gene32861:CDS |
| GTTCTTCAGAACCATCAAGC+TGG | 0.528980 | 3.1:+81837818 | MS.gene32861:CDS |
| TGAAAGGTCCCGGAGGCCGT+GGG | 0.536664 | 3.1:+81842392 | MS.gene32861:CDS |
| GACATTAAGCTGCATTTCAA+CGG | 0.538504 | 3.1:-81843537 | None:intergenic |
| TACATTTACCTTATATATAG+GGG | 0.540407 | 3.1:-81837858 | None:intergenic |
| AATGTGGTCCTGAATCCTGC+TGG | 0.541467 | 3.1:-81843383 | None:intergenic |
| TATTACCACAGCATCAAAAG+TGG | 0.546528 | 3.1:-81839607 | None:intergenic |
| AGGATTTACATGAAAGGTCC+CGG | 0.550432 | 3.1:+81842382 | MS.gene32861:CDS |
| TGAGATGATCACATGTTGAC+TGG | 0.553030 | 3.1:-81843894 | None:intergenic |
| TTTGATACAACTGGAGAATG+TGG | 0.555890 | 3.1:-81843399 | None:intergenic |
| TCATTTGACACCTTATCACT+AGG | 0.568955 | 3.1:-81840619 | None:intergenic |
| TGTACGCAGGTGGGCACCTG+AGG | 0.571914 | 3.1:+81836528 | MS.gene32861:CDS |
| ATGGTCAGAGATGCATATGA+TGG | 0.581139 | 3.1:+81841553 | MS.gene32861:CDS |
| TCAGAACCATCAAGCTGGGA+GGG | 0.589611 | 3.1:+81837823 | MS.gene32861:CDS |
| TACTAGTGATGACTCCTCGC+CGG | 0.591620 | 3.1:+81840648 | MS.gene32861:CDS |
| AATGTCAGATCAAAGCCAGC+AGG | 0.605391 | 3.1:+81843368 | MS.gene32861:intron |
| ACAGATAGGATTTACATGAA+AGG | 0.606780 | 3.1:+81842376 | MS.gene32861:CDS |
| AGGATTTGTCAGCTATCAGA+TGG | 0.611404 | 3.1:+81841534 | MS.gene32861:CDS |
| ATGGTGGAGAAACCCCATCA+AGG | 0.637719 | 3.1:+81835786 | MS.gene32861:CDS |
| TTCAGAACCATCAAGCTGGG+AGG | 0.645173 | 3.1:+81837822 | MS.gene32861:CDS |
| GTTCGCGGTAAAGAGTCTAG+AGG | 0.647921 | 3.1:+81836035 | MS.gene32861:CDS |
| GGATGAAACTTCAATAGACG+TGG | 0.650087 | 3.1:+81837193 | MS.gene32861:CDS |
| GAAAGGTCCCGGAGGCCGTG+GGG | 0.655942 | 3.1:+81842393 | MS.gene32861:CDS |
| TCATACGCTATATATTCCCA+AGG | 0.658657 | 3.1:-81843570 | None:intergenic |
| CAGCTACTTCAACTTCCCCA+CGG | 0.670491 | 3.1:-81842408 | None:intergenic |
| TGTTCTTAATGCACATGATG+GGG | 0.675929 | 3.1:+81840588 | MS.gene32861:CDS |
| ATAATGAATGTACCTTGATG+GGG | 0.703469 | 3.1:-81835798 | None:intergenic |
| ACTAGTACAAACTTCCGGCG+AGG | 0.726490 | 3.1:-81840662 | None:intergenic |
| GATGATCACATGTTGACTGG+AGG | 0.738848 | 3.1:-81843891 | None:intergenic |
| ATTTACATGAAAGGTCCCGG+AGG | 0.781062 | 3.1:+81842385 | MS.gene32861:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 81835776 | 81843908 | 81835776 | ID=MS.gene32861 |
| chr3.1 | mRNA | 81835776 | 81843908 | 81835776 | ID=MS.gene32861.t1;Parent=MS.gene32861 |
| chr3.1 | exon | 81835776 | 81835807 | 81835776 | ID=MS.gene32861.t1.exon1;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81835776 | 81835807 | 81835776 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81835908 | 81836097 | 81835908 | ID=MS.gene32861.t1.exon2;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81835908 | 81836097 | 81835908 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81836475 | 81836549 | 81836475 | ID=MS.gene32861.t1.exon3;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81836475 | 81836549 | 81836475 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81837179 | 81837285 | 81837179 | ID=MS.gene32861.t1.exon4;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81837179 | 81837285 | 81837179 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81837811 | 81837871 | 81837811 | ID=MS.gene32861.t1.exon5;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81837811 | 81837871 | 81837811 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81839380 | 81839439 | 81839380 | ID=MS.gene32861.t1.exon6;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81839380 | 81839439 | 81839380 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81839549 | 81839623 | 81839549 | ID=MS.gene32861.t1.exon7;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81839549 | 81839623 | 81839549 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81840556 | 81840708 | 81840556 | ID=MS.gene32861.t1.exon8;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81840556 | 81840708 | 81840556 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81841520 | 81841574 | 81841520 | ID=MS.gene32861.t1.exon9;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81841520 | 81841574 | 81841520 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81842323 | 81842445 | 81842323 | ID=MS.gene32861.t1.exon10;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81842323 | 81842445 | 81842323 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81843376 | 81843605 | 81843376 | ID=MS.gene32861.t1.exon11;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81843376 | 81843605 | 81843376 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| chr3.1 | exon | 81843885 | 81843908 | 81843885 | ID=MS.gene32861.t1.exon12;Parent=MS.gene32861.t1 |
| chr3.1 | CDS | 81843885 | 81843908 | 81843885 | ID=cds.MS.gene32861.t1;Parent=MS.gene32861.t1 |
| Gene Sequence |
| Protein sequence |
