Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33295.t1 | XP_013617975.1 | 49.1 | 340 | 161 | 5 | 5 | 343 | 6 | 334 | 6.60E-64 | 254.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33295.t1 | A0A2N9I863 | 41.8 | 354 | 172 | 8 | 1 | 339 | 1 | 335 | 7.6e-62 | 247.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene33295 | 0.803812 | 2.92E-49 | -1.69E-46 |
| MS.gene049585 | MS.gene33295 | 0.800497 | 1.40E-48 | -1.69E-46 |
| MS.gene050559 | MS.gene33295 | 0.82 | 8.64E-53 | -1.69E-46 |
| MS.gene050561 | MS.gene33295 | 0.821298 | 4.35E-53 | -1.69E-46 |
| MS.gene050562 | MS.gene33295 | 0.817673 | 2.92E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene33295 | 0.80113 | 1.04E-48 | -1.69E-46 |
| MS.gene052239 | MS.gene33295 | -0.802373 | 5.79E-49 | -1.69E-46 |
| MS.gene05538 | MS.gene33295 | 0.806792 | 6.93E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33295.t1 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 5.90e-32 | 118 |
| MS.gene33295.t1 | MTR_4g037355 | 38.686 | 137 | 62 | 3 | 155 | 290 | 1 | 116 | 5.48e-22 | 90.9 |
| MS.gene33295.t1 | MTR_5g061150 | 35.354 | 99 | 64 | 0 | 52 | 150 | 38 | 136 | 9.22e-18 | 79.3 |
| MS.gene33295.t1 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 143 | 211 | 2 | 69 | 1.38e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 56 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCTTATTGCCCTCATTAA+TGG | 0.201947 | 5.2:-27290088 | MS.gene33295:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 5.2:+27289772 | None:intergenic |
| TGGATCAGTGGAACAAATTA+AGG | 0.229899 | 5.2:-27290068 | MS.gene33295:CDS |
| CAAAAGATCCCAAGATCTTA+TGG | 0.237847 | 5.2:-27290146 | MS.gene33295:CDS |
| GACAAAATTAAAGCAAGATT+TGG | 0.252197 | 5.2:-27290123 | MS.gene33295:CDS |
| GATTCTTGAGGGAGACGATT+TGG | 0.271296 | 5.2:-27290265 | MS.gene33295:CDS |
| CACGACCTCTACCACGAAAA+TGG | 0.272842 | 5.2:+27289656 | None:intergenic |
| CTCAATTAAGTTGTTGTTCC+AGG | 0.274617 | 5.2:+27290314 | None:intergenic |
| GAAGAGGTTATATGACTTAT+AGG | 0.301997 | 5.2:-27289633 | MS.gene33295:CDS |
| ATTTACTGAATACTCTGATT+TGG | 0.325598 | 5.2:-27289878 | MS.gene33295:CDS |
| AGACAGGTAGTGTCATTCTT+TGG | 0.342502 | 5.2:+27289400 | None:intergenic |
| GGGAGATGTCCCAGAGGAAT+TGG | 0.345733 | 5.2:-27290244 | MS.gene33295:CDS |
| ATTCTTGAGGGAGACGATTT+GGG | 0.354692 | 5.2:-27290264 | MS.gene33295:CDS |
| TATTTCTAGAGGGACCTTCC+TGG | 0.364970 | 5.2:+27289557 | None:intergenic |
| CATAGCCACCACGTCCTCTT+TGG | 0.374275 | 5.2:+27289722 | None:intergenic |
| CGTGGTGGCTATGCTCGTTT+TGG | 0.395091 | 5.2:-27289712 | MS.gene33295:CDS |
| TATAACTGAAGTGGAAAAGT+TGG | 0.395452 | 5.2:-27289965 | MS.gene33295:CDS |
| ACAAAATTAAAGCAAGATTT+GGG | 0.407053 | 5.2:-27290122 | MS.gene33295:CDS |
| CAAAGAATATGTGGATGAAA+AGG | 0.429339 | 5.2:-27289452 | MS.gene33295:CDS |
| AAAAGATCCCAAGATCTTAT+GGG | 0.458748 | 5.2:-27290145 | MS.gene33295:CDS |
| AACCATCAGTTGCGTCCTAC+AGG | 0.459311 | 5.2:-27289808 | MS.gene33295:CDS |
| CGTGGTAGAGGTCGTGGAAG+AGG | 0.462783 | 5.2:-27289649 | MS.gene33295:CDS |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 5.2:-27290192 | MS.gene33295:CDS |
| CTGGCGACAACTACATAACC+TGG | 0.502097 | 5.2:-27290332 | MS.gene33295:CDS |
| GATGCGGAAAGAAAGGTCAT+TGG | 0.503149 | 5.2:-27289516 | MS.gene33295:CDS |
| ATGCGGAAAGAAAGGTCATT+GGG | 0.509313 | 5.2:-27289515 | MS.gene33295:CDS |
| ATATGTGGATGAAAAGGAAA+AGG | 0.510737 | 5.2:-27289446 | MS.gene33295:CDS |
| TGTTATAGATGCGGAAAGAA+AGG | 0.513070 | 5.2:-27289523 | MS.gene33295:CDS |
| GGACTAGACAAGATTCTTGA+GGG | 0.521095 | 5.2:-27290276 | MS.gene33295:CDS |
| TTTGATTGGTCATATTTGAG+AGG | 0.522357 | 5.2:+27289610 | None:intergenic |
| TGCAACAACAATATAGAATG+AGG | 0.523271 | 5.2:-27289903 | MS.gene33295:CDS |
| CGTGGACGAGGTAGAAATCA+TGG | 0.535808 | 5.2:-27289685 | MS.gene33295:CDS |
| GTTCCACTGATCCATTAATG+AGG | 0.543612 | 5.2:+27290077 | None:intergenic |
| TTCCACTGATCCATTAATGA+GGG | 0.554709 | 5.2:+27290078 | None:intergenic |
| TAAAAGATACAAAGAATATG+TGG | 0.560646 | 5.2:-27289461 | MS.gene33295:CDS |
| TTCCTGTAGGACGCAACTGA+TGG | 0.562606 | 5.2:+27289806 | None:intergenic |
| AAAATCAGCAGCCTCAAGAC+AGG | 0.562896 | 5.2:+27289384 | None:intergenic |
| GTCATTGGGCTAAAACATGC+AGG | 0.564978 | 5.2:-27289501 | MS.gene33295:CDS |
| AATTGAGTATCTTGCATGTG+AGG | 0.565013 | 5.2:-27290298 | MS.gene33295:CDS |
| ATTGAGTATCTTGCATGTGA+GGG | 0.575966 | 5.2:-27290297 | MS.gene33295:CDS |
| GGATATGCCATTGTTCCTGT+AGG | 0.578642 | 5.2:+27289793 | None:intergenic |
| TTTGGTTGCAGCTCTTCTAG+TGG | 0.579996 | 5.2:-27289860 | MS.gene33295:CDS |
| ACTATCATCATTATTTCTAG+AGG | 0.581988 | 5.2:+27289546 | None:intergenic |
| TGCCCTCATTAATGGATCAG+TGG | 0.582546 | 5.2:-27290080 | MS.gene33295:CDS |
| CTATCATCATTATTTCTAGA+GGG | 0.603915 | 5.2:+27289547 | None:intergenic |
| GCAACAACAATATAGAATGA+GGG | 0.604762 | 5.2:-27289902 | MS.gene33295:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 5.2:+27289759 | None:intergenic |
| TATAATCGCCAAAGAGGACG+TGG | 0.608957 | 5.2:-27289730 | MS.gene33295:CDS |
| GGGACTAGACAAGATTCTTG+AGG | 0.613207 | 5.2:-27290277 | MS.gene33295:CDS |
| GATGATAGTTGTTATAGATG+CGG | 0.615209 | 5.2:-27289532 | MS.gene33295:CDS |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 5.2:-27289736 | MS.gene33295:CDS |
| GTTGCGTCCTACAGGAACAA+TGG | 0.648629 | 5.2:-27289800 | MS.gene33295:CDS |
| GAATGACACTACCTGTCTTG+AGG | 0.649563 | 5.2:-27289395 | MS.gene33295:CDS |
| AATCGCCAAAGAGGACGTGG+TGG | 0.698905 | 5.2:-27289727 | MS.gene33295:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 5.2:+27290184 | None:intergenic |
| CGATTTGGGAGATGTCCCAG+AGG | 0.725435 | 5.2:-27290250 | MS.gene33295:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATTAAAGCAAGATTT+GGG | - | chr5.2:27289607-27289626 | MS.gene33295:CDS | 20.0% |
| !! | TAAAAGATACAAAGAATATG+TGG | - | chr5.2:27290268-27290287 | MS.gene33295:CDS | 20.0% |
| !!! | TTCAAAATTTTAAGCATAAC+TGG | - | chr5.2:27289378-27289397 | MS.gene33295:CDS | 20.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | - | chr5.2:27289851-27289870 | MS.gene33295:CDS | 25.0% |
| ! | CAATCAAACAAAAATCATCA+GGG | - | chr5.2:27290134-27290153 | MS.gene33295:CDS | 25.0% |
| ! | GACAAAATTAAAGCAAGATT+TGG | - | chr5.2:27289606-27289625 | MS.gene33295:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | - | chr5.2:27289735-27289754 | MS.gene33295:CDS | 25.0% |
| !!! | ACTATCATCATTATTTCTAG+AGG | + | chr5.2:27290186-27290205 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | + | chr5.2:27289689-27289708 | None:intergenic | 25.0% |
| !!! | CTATCATCATTATTTCTAGA+GGG | + | chr5.2:27290185-27290204 | None:intergenic | 25.0% |
| !!! | TGTTGAAGGAGATTTTAATA+TGG | - | chr5.2:27290367-27290386 | MS.gene33295:CDS | 25.0% |
| AAAAGATCCCAAGATCTTAT+GGG | - | chr5.2:27289584-27289603 | MS.gene33295:CDS | 30.0% | |
| AACAAAAATCATCAGGGTAA+AGG | - | chr5.2:27290140-27290159 | MS.gene33295:CDS | 30.0% | |
| ATATGTGGATGAAAAGGAAA+AGG | - | chr5.2:27290283-27290302 | MS.gene33295:CDS | 30.0% | |
| CAAAGAATATGTGGATGAAA+AGG | - | chr5.2:27290277-27290296 | MS.gene33295:CDS | 30.0% | |
| CCAATCAAACAAAAATCATC+AGG | - | chr5.2:27290133-27290152 | MS.gene33295:CDS | 30.0% | |
| GAAGAGGTTATATGACTTAT+AGG | - | chr5.2:27290096-27290115 | MS.gene33295:CDS | 30.0% | |
| GATGATAGTTGTTATAGATG+CGG | - | chr5.2:27290197-27290216 | MS.gene33295:CDS | 30.0% | |
| GCAACAACAATATAGAATGA+GGG | - | chr5.2:27289827-27289846 | MS.gene33295:CDS | 30.0% | |
| TATAACTGAAGTGGAAAAGT+TGG | - | chr5.2:27289764-27289783 | MS.gene33295:CDS | 30.0% | |
| TGCAACAACAATATAGAATG+AGG | - | chr5.2:27289826-27289845 | MS.gene33295:CDS | 30.0% | |
| TGGCAAAAATATAACTGAAG+TGG | - | chr5.2:27289755-27289774 | MS.gene33295:CDS | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | + | chr5.2:27289548-27289567 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | + | chr5.2:27289595-27289614 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | + | chr5.2:27289594-27289613 | None:intergenic | 30.0% |
| ! | TATCTTTTACACAGATACTC+TGG | + | chr5.2:27290257-27290276 | None:intergenic | 30.0% |
| ! | TTTGATTGGTCATATTTGAG+AGG | + | chr5.2:27290122-27290141 | None:intergenic | 30.0% |
| !! | CCTGATGATTTTTGTTTGAT+TGG | + | chr5.2:27290136-27290155 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | - | chr5.2:27290158-27290177 | MS.gene33295:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | + | chr5.2:27289960-27289979 | None:intergenic | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | - | chr5.2:27289431-27289450 | MS.gene33295:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | - | chr5.2:27289432-27289451 | MS.gene33295:CDS | 35.0% | |
| CAAAAGATCCCAAGATCTTA+TGG | - | chr5.2:27289583-27289602 | MS.gene33295:CDS | 35.0% | |
| TGGATCAGTGGAACAAATTA+AGG | - | chr5.2:27289661-27289680 | MS.gene33295:CDS | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | - | chr5.2:27290206-27290225 | MS.gene33295:CDS | 35.0% | |
| TTCCACTGATCCATTAATGA+GGG | + | chr5.2:27289654-27289673 | None:intergenic | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | - | chr5.2:27289966-27289985 | MS.gene33295:CDS | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | - | chr5.2:27289967-27289986 | MS.gene33295:CDS | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | + | chr5.2:27289418-27289437 | None:intergenic | 35.0% |
| ! | TTTTCTTTGCCAATTCCTCT+GGG | + | chr5.2:27289497-27289516 | None:intergenic | 35.0% |
| ! | TTTTTCTTTGCCAATTCCTC+TGG | + | chr5.2:27289498-27289517 | None:intergenic | 35.0% |
| !! | AGTCTTATTGCCCTCATTAA+TGG | - | chr5.2:27289641-27289660 | MS.gene33295:CDS | 35.0% |
| ACGCCCTTGATTAAAAGTTG+TGG | + | chr5.2:27289973-27289992 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | - | chr5.2:27289537-27289556 | MS.gene33295:CDS | 40.0% | |
| ATGCGGAAAGAAAGGTCATT+GGG | - | chr5.2:27290214-27290233 | MS.gene33295:CDS | 40.0% | |
| ATTCTTGAGGGAGACGATTT+GGG | - | chr5.2:27289465-27289484 | MS.gene33295:CDS | 40.0% | |
| GGACTAGACAAGATTCTTGA+GGG | - | chr5.2:27289453-27289472 | MS.gene33295:CDS | 40.0% | |
| GGGTAAAGGTAAAAACATCC+AGG | - | chr5.2:27290154-27290173 | MS.gene33295:CDS | 40.0% | |
| GTTCCACTGATCCATTAATG+AGG | + | chr5.2:27289655-27289674 | None:intergenic | 40.0% | |
| ! | AACTTTTCATGCATCTCAGG+TGG | - | chr5.2:27289800-27289819 | MS.gene33295:CDS | 40.0% |
| ! | ACAACTTTTAATCAAGGGCG+TGG | - | chr5.2:27289972-27289991 | MS.gene33295:CDS | 40.0% |
| ! | AGACAGGTAGTGTCATTCTT+TGG | + | chr5.2:27290332-27290351 | None:intergenic | 40.0% |
| ! | CTCAACTTTTCATGCATCTC+AGG | - | chr5.2:27289797-27289816 | MS.gene33295:CDS | 40.0% |
| AAAATCAGCAGCCTCAAGAC+AGG | + | chr5.2:27290348-27290367 | None:intergenic | 45.0% | |
| GAATGACACTACCTGTCTTG+AGG | - | chr5.2:27290334-27290353 | MS.gene33295:CDS | 45.0% | |
| GATGCGGAAAGAAAGGTCAT+TGG | - | chr5.2:27290213-27290232 | MS.gene33295:CDS | 45.0% | |
| GATTCTTGAGGGAGACGATT+TGG | - | chr5.2:27289464-27289483 | MS.gene33295:CDS | 45.0% | |
| GGATATGCCATTGTTCCTGT+AGG | + | chr5.2:27289939-27289958 | None:intergenic | 45.0% | |
| GGGACTAGACAAGATTCTTG+AGG | - | chr5.2:27289452-27289471 | MS.gene33295:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | - | chr5.2:27289993-27290012 | MS.gene33295:CDS | 45.0% | |
| GTCATTGGGCTAAAACATGC+AGG | - | chr5.2:27290228-27290247 | MS.gene33295:CDS | 45.0% | |
| TATAATCGCCAAAGAGGACG+TGG | - | chr5.2:27289999-27290018 | MS.gene33295:CDS | 45.0% | |
| TTTGGTTGCAGCTCTTCTAG+TGG | - | chr5.2:27289869-27289888 | MS.gene33295:CDS | 45.0% | |
| ! | CATGGTCGAAACCATTTTCG+TGG | - | chr5.2:27290062-27290081 | MS.gene33295:CDS | 45.0% |
| ! | CGAAACCATTTTCGTGGTAG+AGG | - | chr5.2:27290068-27290087 | MS.gene33295:CDS | 45.0% |
| ! | TGCCCTCATTAATGGATCAG+TGG | - | chr5.2:27289649-27289668 | MS.gene33295:CDS | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | - | chr5.2:27289975-27289994 | MS.gene33295:CDS | 45.0% |
| !! | TATTTCTAGAGGGACCTTCC+TGG | + | chr5.2:27290175-27290194 | None:intergenic | 45.0% |
| !!! | GAGGCTGCTGATTTTGTTGA+AGG | - | chr5.2:27290353-27290372 | MS.gene33295:CDS | 45.0% |
| AACCATCAGTTGCGTCCTAC+AGG | - | chr5.2:27289921-27289940 | MS.gene33295:CDS | 50.0% | |
| CACGACCTCTACCACGAAAA+TGG | + | chr5.2:27290076-27290095 | None:intergenic | 50.0% | |
| CGTGGACGAGGTAGAAATCA+TGG | - | chr5.2:27290044-27290063 | MS.gene33295:CDS | 50.0% | |
| CTGGCGACAACTACATAACC+TGG | - | chr5.2:27289397-27289416 | MS.gene33295:CDS | 50.0% | |
| GTTGCGTCCTACAGGAACAA+TGG | - | chr5.2:27289929-27289948 | MS.gene33295:CDS | 50.0% | |
| TTCCTGTAGGACGCAACTGA+TGG | + | chr5.2:27289926-27289945 | None:intergenic | 50.0% | |
| ! | CATTTTCGTGGTAGAGGTCG+TGG | - | chr5.2:27290074-27290093 | MS.gene33295:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | - | chr5.2:27290026-27290045 | MS.gene33295:CDS | 50.0% |
| AATCGCCAAAGAGGACGTGG+TGG | - | chr5.2:27290002-27290021 | MS.gene33295:CDS | 55.0% | |
| CATAGCCACCACGTCCTCTT+TGG | + | chr5.2:27290010-27290029 | None:intergenic | 55.0% | |
| GGGAGATGTCCCAGAGGAAT+TGG | - | chr5.2:27289485-27289504 | MS.gene33295:CDS | 55.0% | |
| ! | CGATTTGGGAGATGTCCCAG+AGG | - | chr5.2:27289479-27289498 | MS.gene33295:CDS | 55.0% |
| ! | CGTGGTGGCTATGCTCGTTT+TGG | - | chr5.2:27290017-27290036 | MS.gene33295:CDS | 55.0% |
| ! | GGTGGCTATGCTCGTTTTGG+TGG | - | chr5.2:27290020-27290039 | MS.gene33295:CDS | 55.0% |
| CGTGGTAGAGGTCGTGGAAG+AGG | - | chr5.2:27290080-27290099 | MS.gene33295:CDS | 60.0% | |
| !! | CGTTTTGGTGGTCGTGGACG+AGG | - | chr5.2:27290032-27290051 | MS.gene33295:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 27289357 | 27290394 | 27289357 | ID=MS.gene33295 |
| chr5.2 | mRNA | 27289357 | 27290394 | 27289357 | ID=MS.gene33295.t1;Parent=MS.gene33295 |
| chr5.2 | exon | 27289357 | 27290394 | 27289357 | ID=MS.gene33295.t1.exon1;Parent=MS.gene33295.t1 |
| chr5.2 | CDS | 27289357 | 27290394 | 27289357 | ID=cds.MS.gene33295.t1;Parent=MS.gene33295.t1 |
| Gene Sequence |
| Protein sequence |
