Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33843.t1 | XP_013469751.1 | 97.3 | 186 | 4 | 1 | 1 | 185 | 1 | 186 | 2.10E-88 | 335.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33843.t1 | P11432 | 73.1 | 197 | 40 | 2 | 1 | 185 | 1 | 196 | 1.0e-67 | 257.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33843.t1 | A0A072VP35 | 97.3 | 186 | 4 | 1 | 1 | 185 | 1 | 186 | 1.5e-88 | 335.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene33843 | 0.802281 | 6.04E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene33843 | 0.822036 | 2.94E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene33843 | 0.80764 | 4.58E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene33843 | 0.817003 | 4.13E-52 | -1.69E-46 |
| MS.gene052469 | MS.gene33843 | 0.836847 | 7.36E-57 | -1.69E-46 |
| MS.gene054349 | MS.gene33843 | 0.821075 | 4.90E-53 | -1.69E-46 |
| MS.gene055539 | MS.gene33843 | 0.854563 | 1.13E-61 | -1.69E-46 |
| MS.gene055741 | MS.gene33843 | 0.808648 | 2.79E-50 | -1.69E-46 |
| MS.gene055744 | MS.gene33843 | 0.815984 | 6.98E-52 | -1.69E-46 |
| MS.gene059717 | MS.gene33843 | 0.832611 | 8.56E-56 | -1.69E-46 |
| MS.gene059788 | MS.gene33843 | 0.825711 | 4.04E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene33843 | 0.83893 | 2.15E-57 | -1.69E-46 |
| MS.gene061231 | MS.gene33843 | 0.807139 | 5.85E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33843.t1 | MTR_1g102830 | 97.312 | 186 | 4 | 1 | 1 | 185 | 1 | 186 | 5.01e-129 | 360 |
| MS.gene33843.t1 | MTR_1g102810 | 94.083 | 169 | 10 | 0 | 17 | 185 | 14 | 182 | 8.00e-115 | 324 |
| MS.gene33843.t1 | MTR_1g102780 | 79.888 | 179 | 22 | 1 | 21 | 185 | 22 | 200 | 1.97e-98 | 283 |
| MS.gene33843.t1 | MTR_1g102570 | 79.096 | 177 | 25 | 1 | 21 | 185 | 22 | 198 | 8.24e-97 | 279 |
| MS.gene33843.t1 | MTR_1g102800 | 78.977 | 176 | 25 | 1 | 22 | 185 | 22 | 197 | 1.73e-96 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33843.t1 | AT3G22840 | 51.000 | 200 | 78 | 4 | 1 | 185 | 1 | 195 | 7.98e-59 | 182 |
| MS.gene33843.t1 | AT4G14690 | 51.323 | 189 | 78 | 3 | 11 | 185 | 5 | 193 | 1.37e-54 | 172 |
Find 45 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTCCATTGATCCTTTC+AGG | 0.186558 | 1.3:-70601320 | None:intergenic |
| CAGAAGGGATGTTGTTAAAT+TGG | 0.214729 | 1.3:-70600745 | None:intergenic |
| GTTGTTGACAGTTGCTTCTT+TGG | 0.263072 | 1.3:+70601451 | MS.gene33843:CDS |
| ACACTAACACCTTCAAATAA+TGG | 0.346554 | 1.3:-70601476 | None:intergenic |
| ATTCCAAAGCTCTGCATTTG+AGG | 0.355412 | 1.3:-70601525 | None:intergenic |
| GGTTTGTTCGATCAAATATC+TGG | 0.369233 | 1.3:+70601395 | MS.gene33843:CDS |
| TATGTTGGGTTTGATTGCTT+TGG | 0.370659 | 1.3:+70601559 | MS.gene33843:CDS |
| GGCTGTTACAGAATATGTTA+AGG | 0.394991 | 1.3:+70601580 | MS.gene33843:CDS |
| AATGGAAGGTTGGCCATGAT+TGG | 0.396347 | 1.3:+70601332 | MS.gene33843:CDS |
| TCTTTGGTTCCATTATTTGA+AGG | 0.404737 | 1.3:+70601467 | MS.gene33843:CDS |
| GCTGTTACAGAATATGTTAA+GGG | 0.415066 | 1.3:+70601581 | MS.gene33843:CDS |
| TAGCTTGAAAGTTCGATCTA+TGG | 0.429335 | 1.3:+70600796 | MS.gene33843:CDS |
| GCAGGCTGTGGTGTCACCTT+TGG | 0.429431 | 1.3:-70601084 | None:intergenic |
| TGTATATGCCTAGCTTTAGA+AGG | 0.430319 | 1.3:+70600768 | MS.gene33843:CDS |
| TCTAGGCATGCTTGAAATGC+TGG | 0.450968 | 1.3:-70600717 | None:intergenic |
| TTGTTAAATTGGTTAACTCT+AGG | 0.465217 | 1.3:-70600734 | None:intergenic |
| TGTCCTCAAATGCAGAGCTT+TGG | 0.467503 | 1.3:+70601522 | MS.gene33843:CDS |
| AGTGTTGAATCTAAGTCAAA+AGG | 0.480583 | 1.3:+70601494 | MS.gene33843:CDS |
| GAATGGAAGACTTGCTATGT+TGG | 0.490221 | 1.3:+70601544 | MS.gene33843:CDS |
| CTATAAAACTCTATTAAACA+AGG | 0.499792 | 1.3:-70601610 | None:intergenic |
| AATGGAAGACTTGCTATGTT+GGG | 0.502682 | 1.3:+70601545 | MS.gene33843:CDS |
| TGCAGATACAAAACCAATCA+TGG | 0.506046 | 1.3:-70601345 | None:intergenic |
| TTCGATCAAATATCTGGTGG+TGG | 0.522060 | 1.3:+70601401 | MS.gene33843:CDS |
| GCAGCACCTGAAAGGATCAA+TGG | 0.528131 | 1.3:+70601314 | MS.gene33843:CDS |
| CTAGCATTCCTTCTAAAGCT+AGG | 0.532093 | 1.3:-70600776 | None:intergenic |
| TGAAAGGATCAATGGAAGGT+TGG | 0.548007 | 1.3:+70601322 | MS.gene33843:CDS |
| CACTTGTATACAAGAACCAT+GGG | 0.556587 | 1.3:-70601427 | None:intergenic |
| CTCTACTACTCGTTCACCAA+AGG | 0.565802 | 1.3:+70601107 | MS.gene33843:CDS |
| GGTGAACGAGTAGTAGAGGC+AGG | 0.568710 | 1.3:-70601102 | None:intergenic |
| TTGGTGGGGCAGCACCTGAA+AGG | 0.571883 | 1.3:+70601306 | MS.gene33843:CDS |
| CGAGTAGTAGAGGCAGGCTG+TGG | 0.578503 | 1.3:-70601096 | None:intergenic |
| TCAAATGCAGAGCTTTGGAA+TGG | 0.580798 | 1.3:+70601527 | MS.gene33843:CDS |
| AAGCTAGGCATATACACAGA+AGG | 0.581115 | 1.3:-70600761 | None:intergenic |
| CATGCTTGAAATGCTGGTCA+TGG | 0.581519 | 1.3:-70600711 | None:intergenic |
| GGTGGAATTAGCAAAAGGGC+AGG | 0.590541 | 1.3:+70601373 | MS.gene33843:CDS |
| TTGTTCGATCAAATATCTGG+TGG | 0.594040 | 1.3:+70601398 | MS.gene33843:CDS |
| AGCTAGGCATATACACAGAA+GGG | 0.602384 | 1.3:-70600760 | None:intergenic |
| GTGGAATTAGCAAAAGGGCA+GGG | 0.608172 | 1.3:+70601374 | MS.gene33843:CDS |
| GAAAGTTCGATCTATGGCTG+AGG | 0.614830 | 1.3:+70600802 | MS.gene33843:CDS |
| ACACTTGTATACAAGAACCA+TGG | 0.618963 | 1.3:-70601428 | None:intergenic |
| CTTTGGTGAACGAGTAGTAG+AGG | 0.621298 | 1.3:-70601106 | None:intergenic |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.3:+70601068 | MS.gene33843:CDS |
| CACCTGAAAGGATCAATGGA+AGG | 0.632072 | 1.3:+70601318 | MS.gene33843:CDS |
| TATAAAACTCTATTAAACAA+GGG | 0.645194 | 1.3:-70601609 | None:intergenic |
| TATCTGGTGGTGGAATCCCA+TGG | 0.688884 | 1.3:+70601411 | MS.gene33843:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATATCAAACAAATATTA+AGG | + | chr1.3:70600836-70600855 | MS.gene33843:intron | 10.0% |
| !! | ATCATGATATCATTATATAA+TGG | + | chr1.3:70601249-70601268 | MS.gene33843:intron | 15.0% |
| !!! | GCATATTTAATTTCTATACA+TGG | + | chr1.3:70600882-70600901 | MS.gene33843:intron | 20.0% |
| ! | AATCATAAACGTGTTTGATA+TGG | - | chr1.3:70600916-70600935 | None:intergenic | 25.0% |
| ! | GTCATGTAAAGAATTCATTT+CGG | + | chr1.3:70601192-70601211 | MS.gene33843:intron | 25.0% |
| ! | TTGTTAAATTGGTTAACTCT+AGG | - | chr1.3:70600737-70600756 | None:intergenic | 25.0% |
| ACACTAACACCTTCAAATAA+TGG | - | chr1.3:70601479-70601498 | None:intergenic | 30.0% | |
| AGTGTCTCTTTACATTATTC+AGG | + | chr1.3:70601044-70601063 | MS.gene33843:intron | 30.0% | |
| CTCTTTACATTATTCAGGAA+GGG | + | chr1.3:70601049-70601068 | MS.gene33843:intron | 30.0% | |
| GCTGTTACAGAATATGTTAA+GGG | + | chr1.3:70601581-70601600 | MS.gene33843:CDS | 30.0% | |
| TCTCTTTACATTATTCAGGA+AGG | + | chr1.3:70601048-70601067 | MS.gene33843:intron | 30.0% | |
| TTACATGACTATACTCTTCT+TGG | - | chr1.3:70601181-70601200 | None:intergenic | 30.0% | |
| TTTATTACTATGCTCACCTT+TGG | - | chr1.3:70601126-70601145 | None:intergenic | 30.0% | |
| ! | AGTGTTGAATCTAAGTCAAA+AGG | + | chr1.3:70601494-70601513 | MS.gene33843:CDS | 30.0% |
| ! | TCTTTGGTTCCATTATTTGA+AGG | + | chr1.3:70601467-70601486 | MS.gene33843:CDS | 30.0% |
| !!! | ATTGGTTTTGTATCTGCAAT+GGG | + | chr1.3:70601350-70601369 | MS.gene33843:CDS | 30.0% |
| !!! | GTCGTTGATTTTGTAAATTC+CGG | + | chr1.3:70600963-70600982 | MS.gene33843:intron | 30.0% |
| ACACTTGTATACAAGAACCA+TGG | - | chr1.3:70601431-70601450 | None:intergenic | 35.0% | |
| ATCTATGGCTGAGGTAATTA+AGG | + | chr1.3:70600811-70600830 | MS.gene33843:intron | 35.0% | |
| CACTTGTATACAAGAACCAT+GGG | - | chr1.3:70601430-70601449 | None:intergenic | 35.0% | |
| CAGAAGGGATGTTGTTAAAT+TGG | - | chr1.3:70600748-70600767 | None:intergenic | 35.0% | |
| CATAAACGTGTTTGATATGG+TGG | - | chr1.3:70600913-70600932 | None:intergenic | 35.0% | |
| GGCTGTTACAGAATATGTTA+AGG | + | chr1.3:70601580-70601599 | MS.gene33843:CDS | 35.0% | |
| TAGCTTGAAAGTTCGATCTA+TGG | + | chr1.3:70600796-70600815 | MS.gene33843:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.3:70601559-70601578 | MS.gene33843:CDS | 35.0% | |
| TGCAGATACAAAACCAATCA+TGG | - | chr1.3:70601348-70601367 | None:intergenic | 35.0% | |
| TTGTTCGATCAAATATCTGG+TGG | + | chr1.3:70601398-70601417 | MS.gene33843:CDS | 35.0% | |
| ! | CACAAAGTTTTCGGATTTGA+TGG | + | chr1.3:70601280-70601299 | MS.gene33843:CDS | 35.0% |
| ! | GCAGATAAGCACAAAGTTTT+CGG | + | chr1.3:70601271-70601290 | MS.gene33843:intron | 35.0% |
| ! | GGTTTGTTCGATCAAATATC+TGG | + | chr1.3:70601395-70601414 | MS.gene33843:CDS | 35.0% |
| !! | AATGGAAGACTTGCTATGTT+GGG | + | chr1.3:70601545-70601564 | MS.gene33843:CDS | 35.0% |
| !! | TGTATATGCCTAGCTTTAGA+AGG | + | chr1.3:70600768-70600787 | MS.gene33843:CDS | 35.0% |
| !! | TTTTCGGATTTGATGGCTTT+TGG | + | chr1.3:70601287-70601306 | MS.gene33843:CDS | 35.0% |
| !!! | GATTGGTTTTGTATCTGCAA+TGG | + | chr1.3:70601349-70601368 | MS.gene33843:CDS | 35.0% |
| !!! | TTGGTTTTGTATCTGCAATG+GGG | + | chr1.3:70601351-70601370 | MS.gene33843:CDS | 35.0% |
| AACCTTCCATTGATCCTTTC+AGG | - | chr1.3:70601323-70601342 | None:intergenic | 40.0% | |
| AAGCTAGGCATATACACAGA+AGG | - | chr1.3:70600764-70600783 | None:intergenic | 40.0% | |
| AGCTAGGCATATACACAGAA+GGG | - | chr1.3:70600763-70600782 | None:intergenic | 40.0% | |
| ATTCCAAAGCTCTGCATTTG+AGG | - | chr1.3:70601528-70601547 | None:intergenic | 40.0% | |
| GTTGTTGACAGTTGCTTCTT+TGG | + | chr1.3:70601451-70601470 | MS.gene33843:CDS | 40.0% | |
| TGAAAGGATCAATGGAAGGT+TGG | + | chr1.3:70601322-70601341 | MS.gene33843:CDS | 40.0% | |
| TTCGATCAAATATCTGGTGG+TGG | + | chr1.3:70601401-70601420 | MS.gene33843:CDS | 40.0% | |
| !! | CTAGCATTCCTTCTAAAGCT+AGG | - | chr1.3:70600779-70600798 | None:intergenic | 40.0% |
| !! | GAATGGAAGACTTGCTATGT+TGG | + | chr1.3:70601544-70601563 | MS.gene33843:CDS | 40.0% |
| !! | TCAAATGCAGAGCTTTGGAA+TGG | + | chr1.3:70601527-70601546 | MS.gene33843:CDS | 40.0% |
| !!! | TGGTTTTGTATCTGCAATGG+GGG | + | chr1.3:70601352-70601371 | MS.gene33843:CDS | 40.0% |
| ATGGGGGTGGAATTAGCAAA+AGG | + | chr1.3:70601368-70601387 | MS.gene33843:CDS | 45.0% | |
| CACCTGAAAGGATCAATGGA+AGG | + | chr1.3:70601318-70601337 | MS.gene33843:CDS | 45.0% | |
| CATGCTTGAAATGCTGGTCA+TGG | - | chr1.3:70600714-70600733 | None:intergenic | 45.0% | |
| CTCTACTACTCGTTCACCAA+AGG | + | chr1.3:70601107-70601126 | MS.gene33843:CDS | 45.0% | |
| CTTTGGTGAACGAGTAGTAG+AGG | - | chr1.3:70601109-70601128 | None:intergenic | 45.0% | |
| GAAAGTTCGATCTATGGCTG+AGG | + | chr1.3:70600802-70600821 | MS.gene33843:CDS | 45.0% | |
| GTGGAATTAGCAAAAGGGCA+GGG | + | chr1.3:70601374-70601393 | MS.gene33843:CDS | 45.0% | |
| TCTACAACTGCGAGACGTTA+TGG | - | chr1.3:70600994-70601013 | None:intergenic | 45.0% | |
| TCTAGGCATGCTTGAAATGC+TGG | - | chr1.3:70600720-70600739 | None:intergenic | 45.0% | |
| TGGGGGTGGAATTAGCAAAA+GGG | + | chr1.3:70601369-70601388 | MS.gene33843:CDS | 45.0% | |
| TGTCCTCAAATGCAGAGCTT+TGG | + | chr1.3:70601522-70601541 | MS.gene33843:CDS | 45.0% | |
| ! | AATGGAAGGTTGGCCATGAT+TGG | + | chr1.3:70601332-70601351 | MS.gene33843:CDS | 45.0% |
| ! | TTTTGTATCTGCAATGGGGG+TGG | + | chr1.3:70601355-70601374 | MS.gene33843:CDS | 45.0% |
| !! | CGGATTTGATGGCTTTTGGT+GGG | + | chr1.3:70601291-70601310 | MS.gene33843:CDS | 45.0% |
| !! | GGATTTGATGGCTTTTGGTG+GGG | + | chr1.3:70601292-70601311 | MS.gene33843:CDS | 45.0% |
| !! | TCGGATTTGATGGCTTTTGG+TGG | + | chr1.3:70601290-70601309 | MS.gene33843:CDS | 45.0% |
| AGGGCAGAAAGAGCAACCAA+AGG | + | chr1.3:70601068-70601087 | MS.gene33843:CDS | 50.0% | |
| GCAGCACCTGAAAGGATCAA+TGG | + | chr1.3:70601314-70601333 | MS.gene33843:CDS | 50.0% | |
| GCGAGACGTTATGGATTCTC+CGG | - | chr1.3:70600985-70601004 | None:intergenic | 50.0% | |
| GGTGGAATTAGCAAAAGGGC+AGG | + | chr1.3:70601373-70601392 | MS.gene33843:CDS | 50.0% | |
| !! | TATCTGGTGGTGGAATCCCA+TGG | + | chr1.3:70601411-70601430 | MS.gene33843:CDS | 50.0% |
| GGTGAACGAGTAGTAGAGGC+AGG | - | chr1.3:70601105-70601124 | None:intergenic | 55.0% | |
| CGAGTAGTAGAGGCAGGCTG+TGG | - | chr1.3:70601099-70601118 | None:intergenic | 60.0% | |
| TTGGTGGGGCAGCACCTGAA+AGG | + | chr1.3:70601306-70601325 | MS.gene33843:CDS | 60.0% | |
| !! | GCAGGCTGTGGTGTCACCTT+TGG | - | chr1.3:70601087-70601106 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 70600674 | 70601619 | 70600674 | ID=MS.gene33843 |
| chr1.3 | mRNA | 70600674 | 70601619 | 70600674 | ID=MS.gene33843.t1;Parent=MS.gene33843 |
| chr1.3 | exon | 70600674 | 70600823 | 70600674 | ID=MS.gene33843.t1.exon1;Parent=MS.gene33843.t1 |
| chr1.3 | CDS | 70600674 | 70600823 | 70600674 | ID=cds.MS.gene33843.t1;Parent=MS.gene33843.t1 |
| chr1.3 | exon | 70601066 | 70601128 | 70601066 | ID=MS.gene33843.t1.exon2;Parent=MS.gene33843.t1 |
| chr1.3 | CDS | 70601066 | 70601128 | 70601066 | ID=cds.MS.gene33843.t1;Parent=MS.gene33843.t1 |
| chr1.3 | exon | 70601275 | 70601619 | 70601275 | ID=MS.gene33843.t1.exon3;Parent=MS.gene33843.t1 |
| chr1.3 | CDS | 70601275 | 70601619 | 70601275 | ID=cds.MS.gene33843.t1;Parent=MS.gene33843.t1 |
| Gene Sequence |
| Protein sequence |
